Search Results (190)

Background: Worry proneness has been proposed to influence uncertainty processing; however, its neural mechanisms during different forms of uncertain decision-making remain poorly understood. This study used functional magnetic resonance imaging (fMRI) to investigate behavioral and neural responses associated with risky and ambiguous decision-making and to examine how individual differences in worry proneness relate to uncertainty-related neural processing. Methods: Participants completed a modified ambiguity–risk decision-making task during fMRI scanning. Whole-brain, region-of-interest (ROI), and continuous PSWQ-based correlation analyses were conducted to characterize neural responses associated with uncertainty type and individual differences in worry proneness. Results: Behavioral analyses revealed no significant effects of worry proneness or uncertainty type on gambling choices or reaction times. Whole-brain analyses showed that risky decisions elicited greater activation than ambiguous decisions in the right insula, right inferior parietal lobule, and left supramarginal gyrus, whereas ambiguous decisions preferentially recruited the right superior temporal gyrus. An exploratory peak-level interaction between worry proneness and uncertainty type was identified in the right middle frontal gyrus (BA6), with higher worry proneness associated with lower BA6 activation during risky decision-making. Continuous analyses further showed that BA6 activation during risky decisions was negatively correlated with PSWQ scores. ROI analyses demonstrated greater activation during risky than ambiguous decisions in the bilateral caudate nucleus, left insula, and right inferior frontal gyrus. Conclusions: These findings demonstrate that risky and ambiguous decision-making are associated with partially dissociable patterns of neural activation. Worry proneness was not associated with widespread alterations in behavioral performance or uncertainty-related neural responses; however, exploratory analyses identified localized associations between worry proneness and prefrontal neural responses during risky decision-making. These findings suggest that individual differences in worry proneness may be related to selective variation in specific neural processes rather than broad alterations in the neural systems supporting uncertainty processing. Full article

Background/Objectives: Medulloblastoma is the most common malignant pediatric brain tumor. Although radiotherapy improves survival, it is often associated with neurocognitive impairments affecting language and executive functions in developing brains. We investigated treatment-related changes in task-evoked activation using silent verb-generation functional magnetic resonance imaging (fMRI). Methods: Thirty-one children and adolescents with medulloblastoma enrolled on SJMB12 (18 males; mean age 14.1 ± 4.7 years) completed visual and auditory noun-cued covert verb generation during fMRI before radiotherapy and again within 6 weeks after completing radiotherapy. A two-stage, nested feature-selection framework coupled with a linear Support Vector Machine differentiated pre- from post-irradiation scans using fully nested leave-one-subject-out cross-validation, in which the sign-consistency threshold was re-selected within each training fold, and was evaluated against the 50% chance level of the paired forced-choice design. Results: In fully nested leave-one-subject-out evaluation, the pipeline correctly ordered the paired pre- and post-radiotherapy scans for 22 of 31 participants (71.0%; 95% CI, 52.0–85.8%; one-sided permutation p = 0.015). Descriptive cohort-derived mapping at the modal inner-loop sign-consistency threshold implicated reduced activity in bilateral cerebellar hemispheres (Crus I/II, lobules VI–VIII), left inferior frontal gyrus, left insula, left putamen, supragenual anterior cingulate, and left middle temporal gyrus. Reduced task-negative responses were observed in a large cluster overlapping right precuneus, right superior parietal, right paracentral, and right postcentral cortices, alongside increased engagement of the left supramarginal gyrus and left inferior parietal lobule. Conclusions: In this single-center pilot cohort, silent verb-generation fMRI detected early post-radiotherapy activation changes within cerebellar–cerebral language and control networks. These findings require independent validation, test–retest controls, and longitudinal neurocognitive outcomes before being interpreted as clinical biomarkers. Full article

Background: The frontal aslant tract (FAT) is a white matter pathway associated with language and executive functions. The aim of the study was to identify and analyze this tract using DTI tractography. Methods: The study included 26 healthy adults. Probabilistic multi-fiber tractography and deterministic single-fiber tractography were applied. The seed region (ROI) was defined in the inferior frontal gyrus, and the target region in the superior frontal cortex. FA, MD, and tract volume were analyzed. Results: The FAT was reconstructed in all participants using probabilistic analysis. The left FAT showed higher FA (p = 0.012), greater volume (p = 0.008), and lower MD (p = 0.021) than the right. Probabilistic tractography demonstrated greater tract volume compared to deterministic tractography. Conclusions: The FAT can be identified using DTI tractography. Probabilistic tractography produced more extensive FAT reconstructions than deterministic tractography; however, anatomical accuracy was not directly assessed. Full article

Foreign language reading comprehension is a critical indicator of university students’ academic cognitive ability and global competence, yet effectively assessing their latent learning potential remains a major challenge. Therefore, from a neurocognitive perspective, this study combined functional near-infrared spectroscopy (fNIRS) with dynamic assessment (DA) to investigate the activation characteristics of the left frontal and temporal regions in non-English majors during reading tasks across two difficulty levels (CET-4/CET-6) and two assessment conditions (static/dynamic testing). The results showed that reading tasks activated the dorsolateral prefrontal cortex and middle temporal gyrus. Compared with CET-4, the more difficult CET-6 task showed a broader descriptive cortical activation pattern. Notably, mediated prompts were associated with different behavioral and activation-frequency patterns across task difficulty levels: in the CET-4 task, dynamic assessment showed higher normalized activation-frequency values than static assessment in areas corresponding to the middle and inferior frontal gyrus, accompanied by rising prompt scores; however, the CET-6 task exhibited the opposite pattern. These findings suggest that mediated prompts may be more effective in stimulating learning potential when task difficulty aligns with the learner’s zone of proximal development. Overall, by considering behavioral dynamic-assessment indicators in foreign-language reading together with fNIRS-based cortical activation patterns, this exploratory study offers preliminary insights into learning potential, personalized instructional interventions, and academic cognitive assessment. Full article

The link between the olfactory system and psychiatric disorders is well documented; however, the neurological effects of insect-derived scents on human brain function remain unexplored. This study examined the neurological response to scented insects using functional magnetic resonance imaging (fMRI) to evaluate their potential as a novel animal-assisted intervention (AAI) for mental health. In this controlled, crossover trial, the olfactory effects of scented insects (Poecilocoris splendidulus Esaki) were compared with a control scent (alcohol) in 29 psychologically healthy adults. Brain activation differences were assessed using paired t-tests with false discovery rate correction, followed by multiple regression analyses incorporating psychometric assessments. Compared with the control scent, the insect scent elicited greater activation across sensory, emotional, and memory-related brain networks, including the thalamus, insula, hippocampus, and prefrontal cortex (p < 0.05). Furthermore, individual psychometric variations influenced these responses: higher depressive symptoms were associated with reduced activation in the anterior cingulate cortex, while elevated stress and anxiety correlated with heightened activity in the hippocampus and inferior frontal gyrus. These findings suggest that insect-derived scents induce distinct neurological responses linked to emotional processing, supporting their potential application in therapeutic interventions for mental health. Full article

Background/Objectives: Stroke-related aphasia significantly impairs communication and quality of life (QoL). Constraint-induced aphasia therapy (CIAT) and repetitive transcranial magnetic stimulation (rTMS) are evidence-based approaches targeting language recovery. No combined protocol exists for Greek, a morphologically complex language. The present study was designed as a feasibility pilot, with the primary objective of determining if a modified CIAT (mCIAT) protocol, with or without continuous theta burst stimulation (cTBS), could be delivered safely and acceptably in a Cypriot clinical context, while also reporting the first mCIAT protocol adapted for Greek. Methods: A single-subject experimental design (SSED) was employed. Two participants (PAs) with chronic anomic aphasia underwent a 10-day treatment protocol. PA1 received sham cTBS plus mCIAT-GR; PA2 received real cTBS (to the right inferior frontal gyrus) plus mCIAT. Multiple language, cognitive, and QoL measures were administered across three baselines and two follow-up time points. Results: Both PAs completed the full protocol with 100% session adherence and no adverse effects, establishing the feasibility of delivery. Neither participant showed improvements in standardized expressive language measures following mCIAT with or without cTBS; Standard Error of Measurement (SEM) analysis confirmed that observed score fluctuations on most measures fell within expected measurement noise. PA2 showed improvement in QoL (SAQOL-39g) domains at the two-year follow-up, though this finding requires cautious interpretation given the follow-up interval and regression-to-mean considerations. Conclusions: The Greek mCIAT protocol is feasible and safe to deliver in a Cypriot clinical setting. The absence of clear language improvements may reflect several factors, including ceiling effects, limited assessment sensitivity, insufficient treatment dosage, or limited intervention effects. These findings should be interpreted cautiously and require confirmation in larger, adequately powered studies. Full article

Previous research on the relative roles of maternal and grandmaternal caregiving in child development has been limited by the “maternal advantage hypothesis” and the “grandmaternal advantage hypothesis”. This study posited the “mother–grandmother relative advantage hypothesis” and aimed to evaluate the relative influence of maternal and grandmaternal caregiving in children’s cognitive and emotional outcomes at both behavioral and neural levels. This study comprised two components. In Study 1, a total of 33 children from mother-led or grandmother-led caregiving families were asked to perform a working memory task, aiming to investigate the behavioral and neural differences in cognitive outcomes between the two caregiving groups. In Study 2, a total of 31 children from similar family backgrounds were asked to complete an emotional recognition task, which was designed to examine the behavioral and neural differences in emotional outcomes between the groups. At a behavioral level, grandmaternal rather than maternal caregiving was associated with children’s working memory performance, whereas maternal rather than grandmaternal caregiving was associated with children’s emotional recognition performance. At a neural level, compared to children in the grandmaternal caregiving group, those in the maternal caregiving group showed increased functional connectivity with the right middle frontal gyrus and the left superior frontal gyrus as seeds during working memory tasks and increased functional connectivity with the right inferior occipital gyrus and left precentral gyrus as seeds during emotional recognition tasks. Our findings reveal a complementary pattern of female caregiving effects, supporting “mother–grandmother relative advantage hypothesis”. Full article

Background/Objectives: Epilepsy is a chronic brain disease characterized by recurrent epileptic seizures. It affects roughly 50 million people worldwide and around one third of the patients have drug-resistant epilepsy (DRE). The current study aimed to find differences in the whole-brain functional connectivity (FC) in patients with DRE compared to patients with well-controlled epilepsy (WCE) and healthy controls (HCs). Methods: This explorative, cross-sectional study included 92 participants (n_DRE = 30; n_WCE = 30; n_HC = 32) who underwent resting-state functional magnetic resonance imaging (fMRI). The CONN Toolbox was used to process and analyze the FC changes among the three groups. Results: There was a statistically significant increase of the FC between the left lateral prefrontal cortex, left inferior temporal gyrus (temporo-occipital), left lobules IV and V of the cerebellum and multiple cortical and subcortical structures in patients with DRE as opposed to WCE and HC. On the other hand, decreased FC was observed between three seeds (the posterior cingulate cortex, precuneus cortex, the right planum polare) and different frontal, temporal and occipital regions. Interestingly, the right nucleus accumbens (r_NAc) showed increased FC with the inferior frontal gyrus in DRE compared to WCE, whereas the r_NAc-left precentral gyrus FC was reduced in DRE as opposed to HC. Conclusions: The acquired information offers valuable insights into the neuronal networks associated with DRE. These data could be used for advancing diagnostic accuracy and future therapeutic strategies. Full article

Background/Objectives: The right inferior frontal gyrus (rIFG) is a key node of the neural network underlying inhibitory control and a promising target for non-invasive brain stimulation. Here, we investigated whether an accelerated anodal transcranial direct current stimulation (tDCS) protocol over the rIFG could produce cumulative improvements in response inhibition. This issue is particularly relevant because cumulative effects of accelerated stimulation cannot be taken for granted, and repeated administrations may lead to progressive improvement, saturation, or no measurable offline persistence. In addition, sham-controlled accelerated studies remain limited. Methods: Twenty-two healthy participants underwent three stimulation sessions within the same day. Inhibitory control was assessed using the Stop-Signal task, while Stop-Signal Reaction Time (SSRT) and mean error rate were analyzed by means of linear mixed-effects models. Results: Results showed a significant effect of time and a significant stimulation-by-time interaction across the three sessions, indicating a progressive reduction in SSRT during the accelerated protocol. Thus, inhibitory control performance improved as stimulation sessions increased. Conclusions: These findings suggest that accelerated anodal tDCS over the rIFG can induce cumulative short-term improvements in inhibitory control. The results support the relevance of the rIFG as a neuromodulation target, while highlighting the importance of interindividual variability and the need for more translationally oriented protocols. Full article

Background/Objectives: A current debate addresses where syntactic Merge primarily resides: the left-hemisphere posterior inferior frontal gyrus (IFG) or the temporo-parietal cortex. For proponents of the former, the temporo-parietal cortex supports more effortful processing; for the latter, the IFG supports integration and conflict resolution. We examine aperiodic activity in processing wh-filler-gap dependencies in French for evidence from network dynamics addressing engagement in syntax across L1 and L2. Methods: We extracted aperiodic activity 1/f components (considering offsets as a reflection of neuronal spiking and exponents as a reflection of excitatory–inhibitory balance) out of power spectrum density at 0.5–40 Hz across occipital and bilateral frontal and temporo-parietal regions of interest (ROIs) in reading. Results: Greater exponents arose in temporo-parietal than frontal ROIs in L1 and L2, with strong spiking and regulation suggested by greater offsets and exponents in the occipital ROI in L2—unlike L1—and with potential modulation by L1–L2 representation overlaps. These patterns suggest distributed cell assemblies for L1 and L2 processing. Increased regulation in temporo-parietal ROIs across L1 and L2 cell assemblies might suggest a structural function across temporo-parietal cortices in syntactic processing. Conclusions: Aperiodic activity reflecting connectivity in L1 and L2 processing supports distinct L1 and L2 cell assemblies, with L2 patterns suggesting potential overlap between L1 and L2 circuit modules. Greater exponents in bilateral temporo-parietal ROIs across L1 and L2 indicate increased regulation, supporting the engagement of lateralized temporo-parietal cortices in computations. These effects are discussed by considering advances in syntactic theory and the biology of language readiness. Full article

Background: Action observation (AO) and motor imagery (MI) are widely used cognitive training strategies. Recent evidence suggests that their combination may enhance motor simulation through synergistic neural mechanisms. However, the effects of this approach on complex whole-body movements in elite athletes remain unclear. Methods: Twenty-seven elite ski jumpers performed AO, MI, and concurrent AO + MI tasks, while cortical hemodynamic responses were recorded using functional near-infrared spectroscopy (fNIRS). Channel-level changes in oxygenated hemoglobin (ΔHbO) were analyzed using one-sample and paired-sample t-tests, with false discovery rate (FDR) correction applied for multiple comparisons. Additionally, mixed-design ANOVAs were conducted to examine the potential modulation of athlete level (master-level vs. first-class). Results: Significant activation was observed only in the AO + MIcondition after FDR correction, primarily in channels corresponding to the precentral gyrus, postcentral gyrus, and middle frontal gyrus. No channels in the AO and MI conditions survived FDR correction. Between-condition comparisons revealed significant differences in several channels located in frontoparietal regions, including the inferior parietal lobule, supramarginal gyrus, and middle frontal gyrus, with AO + MIgenerally showing stronger responses. No significant effects related to athlete level were found. Conclusions: These findings indicate that concurrent AO + MI is more effective than AO or MI alone in eliciting cortical activation in elite ski jumpers. This may reflect enhanced engagement of frontoparietal networks involved in action representation and visuomotor integration. These results may be compatible with a neural efficiency interpretation in highly trained athletes, although further studies with behavioral outcomes and broader skill-level comparisons are needed. AO + MI may represent a promising strategy for off-snow cognitive training in high-risk sports. Full article

Background/Objectives: Intentional inhibition reflects voluntary control abilities and is assumed to be an indicator of overweight. The medial frontal cortex is an important brain region associated with intentional inhibition. Nevertheless, it is uncertain whether being overweight is connected to impaired food-related intentional inhibition (FII), and if so, what its underlying neural correlates are. The present study therefore aims to provide increased support for overweight due to impairment of FII. Methods: Firstly, 55 overweight and 45 normal-weight college students (Sample 1) were instructed to perform a go/no-go/choose task, which included a resting-state fMRI. Neural correlates of FII were examined using regional homogeneity (ReHo) analyses. Subsequently, an additional 180 undergraduates (87 overweight and 93 normal-weight; Sample 2) were examined to ascertain the differences in resting-state functional connectivity (rsFC) between overweight and normal-weight participants. The study also investigated whether restrained eating mediated the effect of rsFCs on one-year body index changes. Results: FII demonstrated a positive correlation with the cerebellum, inferior temporal gyrus, orbitofrontal cortex, inferior frontal gyrus, and cingulate gyrus. Additionally, in comparison with participants with normal weight, overweight participants demonstrated diminished rsFC between the FII-related areas and the postcentral gyrus, while heightened rsFC strengths were found between these areas and the middle temporal gyrus and precuneus. Furthermore, mediation analyses demonstrated that cingulate–precuneus connectivity is linked to fat mass index change a year later through restrained eating. Conclusions: FII was associated with connectivity between brain regions involved in inhibitory control and maladaptive eating. Furthermore, we investigated how these connectivity patterns could potentially affect future body fat loss through restrained eating. Full article

Background/Objectives: Children with Autism Spectrum Disorder (ASD, here on termed autistic children) exhibit motor difficulties in social and non-social contexts. Although previous studies have reported behavioral and neural characteristics, their relationship remains largely unexplored. The current study aimed to investigate the behavioral and neural mechanisms underlying interpersonal synchrony in autistic children using simultaneous kinematic and Functional Near-Infrared Spectroscopy (fNIRS) recordings. Methods: Fifty-eight autistic or non-autistic children participated (mean age = 10.1, standard error = 0.3). fNIRS and an inertial measurement unit were used simultaneously to record the neural activity over frontotemporal and parietal regions and arm movement kinematics during a reach-to-clean-up task across three conditions: Watch—the child observed the tester clean up the blocks; Do—the child cleaned up the blocks independently; and Together—the child and tester cleaned up the blocks synchronously. Results: Behaviorally, autistic children demonstrated longer movement displacement, higher average velocity and acceleration, and a greater number of movement units. In terms of cortical activation, autistic children showed hypoactivation in the bilateral precentral gyrus and right inferior parietal lobe, along with hyperactivation in the right middle frontal gyrus, left inferior frontal gyrus, and left inferior parietal lobule. Correlations between kinematic and neural measures suggest that autistic children rely more on online/feedback control to compensate for reduced feedforward control. Conclusions: This study reveals unique compensatory strategies in autistic children, highlighting the connections between neural and behavioral characteristics. These findings have strong potential to inform the development of ASD screening tools and to guide targeted intervention strategies. Full article

Background and Objectives: Cortical plasticity assessment using navigated transcranial magnetic stimulation (nTMS) represents a promising non-invasive strategy for predicting reorganisation of cortical circuits in neuro-oncological patients. This study examined how glioma grade influences cognitive network reorganisation by multiparametric analysis. Materials and Methods: We conducted a prospective monocentric study at the Neurosurgical Department in Florence and a comparative analysis of motor (M), language (Ln), calculation (C), and visuo-spatial functions (VS) between patients with low-grade gliomas (LGGs) and high-grade gliomas (HGGs) undergoing pre- and postoperative nTMS mapping (at 5 ± 2, 30 ± 10, and 90 ± 10 days of follow-up). Results: Between January2024 and September 2025, we enrolled 69 patients, and the total number of nTMS mapping procedures was 70: one relapse, (M:F = 345:365), level of scholarship 8–15 years, 21 LGGs, 30 HGGs, 19 non-glial lesions (excluded), left lesions n = 37, right lesions n = 31, bilateral n = 2, bi-hemispheric nTMS = 80%. Considering LGGs and HGGs, the major motor function displacement was obtained in the right hemisphere (Rh; predominantly for HGGs 64 mm vs. LGGs 39 mm), with more restrained displacement in the left hemisphere (Lh; LGGs 20 mm vs. HGGs 21 mm). For Ln, displacement was higher for HGGs (57 mm vs. LGGs 31 mm). However, surprisingly for HGGs in the Lh, the displacement was more significant (60 mm), whereas for LGGs it was major in the Rh (~80 mm). For C, displacement for HGGs was 72 mm Lh vs. 48.11 mm Rh, and for LGGs 50 mm Lh vs. 41 mm Rh. Insufficient data were obtained for the network. Qualitative analyses further characterised this reorganisation: motor f. demonstrated reshaping around the primary motor cortex; linguistic f. displaced from temporo-parietal areas to the inferior frontal gyrus; calculation and VS functions reorganised within frontoparietal circuits. The correlation between cognitive results and BPI revealed that higher BPI values were associated with prolonged recovery periods. Nevertheless, functional recovery was achieved in up to 90% of patients across all assessed functions. Conclusions: We propose non-invasively measuring cortical plasticity across different cognitive domains with a quantitative–qualitative framework for assessing functional reorganisation with a multimodal assessment in glioma patients. Full article

Background: Frequent nocturnal arousals are a core feature of comorbid insomnia and obstructive sleep apnea (COMISA), yet the underlying central mechanisms remain unclear. Identifying brain functional correlates of nocturnal awakenings may help clarify arousal-related mechanisms and inform potential interventional targets. Methods: A total of 99 participants (COMISA, insomnia alone, OSA alone, and healthy controls) underwent clinical assessments, polysomnography, and brain magnetic resonance imaging (MRI). MRI metrics were compared across groups, followed by correlation and regression analyses with the arousal index, adjusting for respiratory events and insomnia-related factors. Results: Patients with COMISA exhibited more severe insomnia symptoms, greater daytime dysfunction, and more frequent nocturnal awakenings than those with insomnia alone, although their arousal index did not differ from that of the OSA group. Patients with COMISA exhibited altered activity in the right cerebellar lobule VIII (Cerebelum_8_R), left middle temporal gyrus, and right inferior frontal gyrus, opercular part. Lower fractional amplitude of low-frequency fluctuations (fALFF) in the Cerebelum_8_R was associated with a higher arousal index. This association remained significant after controlling for insomnia severity and sleep efficiency but was attenuated after adjustment for AHI. Conclusions: Reduced functional activity in the Cerebelum_8_R was independently associated with sleep fragmentation in COMISA, independent of insomnia severity but potentially mediated by respiratory events. These findings suggest this region may be involved in arousal-related neural regulation and could represent a therapeutic target for the complex symptoms of COMISA. Trial Registration: Chinese Clinical Trial Registry, ChiCTR2500095809. Full article