Search Results (31)

Occupational health monitoring in demolition environments requires precise detection of blast-dust-induced pulmonary pathologies. However, it is often hindered by challenges such as contaminated imaging biomarkers, limited access to medical resources in mining areas, and opaque AI-based diagnostic models. This study presents a novel computational framework that combines industrial-grade robustness with clinical interpretability for the diagnosis of pulmonary nodules. We propose a hybrid framework that integrates morphological purification techniques (multi-step filling and convex hull operations) with multi-dimensional features fusion (radiomics + lightweight deep features). To enhance computational efficiency and interpretability, we design a soft voting ensemble classifier, eliminating the need for complex deep learning architectures. On the LIDC-IDRI dataset, our model achieved an AUC of 0.99 and an accuracy of 0.97 using standard clinical-grade hardware, outperforming state-of-the-art (SOTA) methods while requiring fewer computational resources. Ablation studies, feature weight maps, and normalized mutual information heatmaps confirm the robustness and interpretability of the model, while uncertainty quantification metrics such as the Brier score and Expected Calibration Error (ECE) better validate the model’s clinical applicability and prediction stability. This approach effectively achieves resource-accuracy co-optimization, maintaining low computational costs, and is highly suitable for resource-constrained clinical environments. The modular design of our framework also facilitates extensions to other medical imaging domains without the need for high-end infrastructure. Full article

This study investigates how urban decline and intensifying flood hazards interact to threaten Japan’s urban environments, focusing on three main dimensions. First, a fine-scale analysis of spatial shrinkage was conducted using transition potential maps generated with a maximum entropy classifier. This approach enabled the identification of neighborhoods at high risk of future abandonment, revealing that peripheral districts, such as Hirakue-cho and Shimoirino-cho, are especially susceptible due to their distance from central amenities. Second, this study analyzed the 2019 Naka River flood induced by Typhoon Hagibis, evaluating water detection performance through both a U-Net-based deep learning model applied to Sentinel-1 SAR imagery in ArcGIS Pro and the DioVISTA Flood Simulator. While the SAR-based approach excelled in achieving high accuracy with a score of 0.81, the simulation-based method demonstrated higher sensitivity, emphasizing its effectiveness in flagging potential flood zones. Third, forward-looking scenarios under Representative Concentration Pathways (RCP) 2.6 and RCP 8.5 climate trajectories were modeled to capture the potential scope of future flood impacts. The primary signal is that flooding impacts 3.2 km² of buildings and leaves 11 of 82 evacuation sites vulnerable in the worst-case scenario. Japan’s proven disaster expertise can still jolt adaptation toward greater flexibility. Adaptive frameworks utilizing real-time and predictive insights powered by remote sensing, GIS, and machine intelligence form the core of proactive decision-making. By prioritizing the repositioning of decaying suburbs as disaster prevention hubs, steadily advancing hard and soft measures to deployment, supported by the reliability of DioVISTA as a flood simulator, and fueling participatory, citizen-led ties within a community, resilience shifts from a reactive shield to a living ecosystem, aiming for zero victims. Full article

Permanent engravings on contact lenses provide information about the manufacturing process and lens positioning when they are placed on the eye. The inspection of their morphological characteristics is important, since they can affect the user’s comfort and deposit adhesion. Therefore, an inverted wavefront holoscope (a lensless microscope based on Gabor’s principle of in-line digital holography) is explored for the characterization of the permanent marks of soft contact lenses. The device, based on an in-line transmission configuration, uses a partially coherent laser source to illuminate the soft contact lens placed in a cuvette filled with a saline solution for lens preservation. Holograms were recorded on a digital sensor and reconstructed by back propagation to the image plane based on the angular spectrum method. In addition, a phase-retrieval algorithm was used to enhance the quality of the recovered images. The instrument was experimentally validated through a calibration process in terms of spatial resolution and thickness estimation, showing values that perfectly agree with those that were theoretically expected. Finally, phase maps of different engravings for three commercial soft contact lenses were successfully reconstructed, validating the inverted wavefront holoscope as a potential instrument for the characterization of the permanent marks of soft contact lenses. To improve the final image quality of reconstructions, the geometry of lenses should be considered to avoid induced aberration effects. Full article

Previously, we introduced photomagnetic imaging (PMI) that synergistically utilizes laser light to slightly elevate the tissue temperature and magnetic resonance thermometry (MRT) to measure the induced temperature. The MRT temperature maps are then converted into absorption maps using a dedicated PMI image reconstruction algorithm. In the MRT maps, the presence of abnormalities such as tumors would create a notable high contrast due to their higher hemoglobin levels. In this study, we present a new artificial intelligence-based image reconstruction algorithm that improves the accuracy and spatial resolution of the recovered absorption maps while reducing the recovery time. Technically, a supervised machine learning approach was used to detect and delineate the boundary of tumors directly from the MRT maps based on their temperature contrast to the background. This information was further utilized as a soft functional a priori in the standard PMI algorithm to enhance the absorption recovery. Our new method was evaluated on a tissue-like phantom with two inclusions representing tumors. The reconstructed absorption map showed that the well-trained neural network not only increased the PMI spatial resolution but also improved the accuracy of the recovered absorption to as low as a 2% percentage error, reduced the artifacts by 15%, and accelerated the image reconstruction process approximately 9-fold. Full article

We investigated the immediate molecular consequences of traumatic brain injuries (TBIs) using a novel proteomics approach. We simulated TBIs using an innovative laboratory apparatus that employed a 5.1 kg dummy head that held neuronal cells and generated a ≤4000 g-force acceleration upon impact. A Proteome Integral Solubility Alteration (PISA) assay was then employed to monitor protein solubility changes in a system-wide manner. Dynamic impacts led to both a reduction in neuron viability and massive solubility changes in the proteome. The affected proteins mapped not only to the expected pathways, such as those of cell adhesion, collagen, and laminin structures, as well as the response to stress, but also to other dense protein networks, such as immune response, complement, and coagulation cascades. The cellular effects were found to be mainly due to the shockwave rather than the g-force acceleration. Soft materials could reduce the impact’s severity only until they were fully compressed. This study shows a way of developing a proteome-based meter for measuring irreversible shockwave-induced cell damage and provides a resource for identifying protein biomarkers of TBIs and potential drug targets for the development of products aimed at primary prevention and intervention. Full article

The concept of continuity in topological spaces has a very important place. For this reason, a great deal of work has been done on continuity, and many generalizations of continuity have been obtained. In this work, we seek to find a new approach to the study of soft continuity in soft topological spaces in connection with an induced mapping based on soft sets. By defining the *-image of a soft set, we define an induced soft mapping and present its related properties. To elaborate on the obtained results and relationships, we furnish a number of illustrative examples. Full article

Biological invasions are a human-induced environmental disturbance that can cause major changes in ecosystem structure and functioning. Located in the northeastern Mediterranean basin, the Aegean Sea is a hotspot of biological invasions. Although the presence of alien species in the Aegean has been studied and monitored, no assessment has been conducted on their cumulative impacts on native biodiversity. To address this gap, we applied the CIMPAL index, a framework developed for mapping the cumulative impacts of invasive species, to identify the most affected areas and habitat types and determine the most invasive species in the region. Coastal areas showed stronger impacts than the open sea. The highest CIMPAL scores were four times more frequent in the South than in the North Aegean. Shallow (0–60 m) hard substrates were the most heavily impacted habitat type, followed by shallow soft substrates and seagrass meadows. We identified Caulerpa cylindracea, Lophocladia lallemandii, Siganus luridus, Siganus rivulatus, and Womersleyella setacea as the most impactful species across their range of occurrence in the Aegean but rankings varied depending on the habitat type and impact indicator applied. Our assessment can support marine managers in prioritizing decisions and actions to control biological invasions and mitigate their impacts. Full article

The emission of nanoscale particles from the surfaces of dental implants leads to the cumulative effect of particle complexes in the bone bed and surrounding soft tissues. Aspects of particle migration with the possibility of their involvement in the development of pathological processes of systemic nature remain unexplored. The aim of this work was to study protein production during the interaction of immunocompetent cells with nanoscale metal particles obtained from the surfaces of dental implants in the supernatants. The ability to migrate nanoscale metal particles with possible involvement in the formation of pathological structures, in particular in the formation of gallstones, was also investigated. The following methods were used: microbiological studies, X-ray microtomography, X-ray fluorescence analysis, flow cytometry, electron microscopy, dynamic light scattering, and multiplex immunofluorescence analysis. For the first time, titanium nanoparticles in gallstones were identified by X-ray fluorescence analysis and electron microscopy with elemental mapping. The multiplex analysis method revealed that the physiological response of the immune system cells, in particular neutrophils, to nanosized metal particles significantly reduced TNF-a production both through direct interaction and through double lipopolysaccharide-induced signaling. For the first time, a significant decrease in TNF-a production was demonstrated when supernatants containing nanoscale metal particles were co-cultured with proinflammatory peritoneal exudate obtained from the peritoneum of the C57Bl/6J inbred mice line for one day. Full article

Aim: Soft denture lining materials are susceptible to be colonized by different microorganisms, especially by Candida albicans (C. albicans), causing denture-induced stomatitis. This study was designed to evaluate the effectiveness of incorporating titanium dioxide nanoparticles (TiO₂ NPs) into a soft denture liner towards reducing microbial activity. Method: A total of 40 PEMA-TiO₂ nanocomposites samples were fabricated by adding 0.0 wt.% (control), 1.0 wt.%, 1.5 wt.%, and 2 wt.% TiO₂ NPs to a heat cured soft denture lining material (polyethyl methacrylate, PEMA). The prepared samples were divided into four groups (n = 10) according to the content of TiO₂ NPs. The uniformity of TiO₂ NPS distribution within the denture liner matrix was assessed using a Scanning Electron Microscope (SEM). The viable count of C. albicans was evaluated to test the antifungal resistance of the developed composite. Results: The SEM images showed fairly homogeneous dispersion, with patches of TiO₂ NPs agglomeration within the PEMA matrix and an increasing concentration of NPs with higher NP content. The particle map and EDX analysis confirmed the evidence of the TiO₂ NPs. The mean viable count results for the control (0.0 wt.%) and 1.0 wt.%, 1.5 wt.%, and 2 wt.% TiO₂ groups were 139.80, 12.00, 6.20, and 1.00, respectively, with a significant difference from the control group (p < 0.05). The antifungal activity also increased with the increase in the concentration of TiO₂ NPs. Conclusions: The addition of TiO₂ NPs into a heat-cured soft denture liner provided antifungal activity as evidenced by the reduced colonization of C. albicans. The antimicrobial activity of the liner material increased with the increased concentration of TiO₂ NPS. Full article

Single-event transient (SET)-induced soft errors are becoming a more significant threat to the reliability of electronic systems in space, especially for advanced technologies. The SET pulse width, which is vulnerable to SET propagation, is a critical parameter for developing SET mitigation techniques. This paper investigates the pulse-broadening effect in the process of SET propagation in logic circuits and the SET-sensitive region distribution in the layout using the pulsed-laser mapping technique in logic circuits implemented with 28 nm Ultra-Thin Body and BOX (UTBB) FDSOI technology. The experiments were carried out at the Naval Research Laboratory (NRL) to measure the SET-induced errors and map the SET-sensitive region distribution at various clock frequencies and laser energy levels. The results illustrate that the number of errors increases with the clock frequency and energy for combinational logic circuits and that the flip-flop SEU rate is less sensitive to clock frequency. The SET pulse-broadening effect was also observed using SET mapping for an OR gate chain at different laser energy levels. In addition, the simulation results revealed the mechanism of the SET pulse-broadening effect in an OR gate chain. Full article

This research presents the microstructural and mechanical evolution throughout the welded seam of an austenitic stainless steel (ASS) tube. It was found that the main hardness decrement occurred in the fusion zone (FZ), followed by the heat-affected zone (HAZ) and the base material (BM). Optical microscopy indicated a dendritic structure in FZ and heterogeneous austenitic grain size from the HAZ towards the BM, ranging from 100 µm to 10 µm. The welding process generated an intense texture around the FZ and the HAZ, while the BM still showed an extrusion-like texture. In terms of mechanical behavior, the largest austenite grain size in the FZ led to the lowest strength and ductility of all zones due to the earliest strain localization manifested by heterogeneous strain distribution. However, the strain localization in all zones appeared after 0.4 true strain, indicating an overall good ductility of the seam. These high values were related to two microstructure characteristics: (1) the 10% δ-ferrite after solidification in the FZ favored by the $C{r}_{eq}/N{i}_{eq}=1.67$ relationship that delayed the crack propagation along the austenite grains and (2) the heterogeneous microstructure made up of soft austenite and hard martensite in the HAZ and BM producing multiple strain concentrations. Kernel Average Misorientation (KAM) maps obtained by Electron Back-Scattering Diffraction (EBSD) allowed observing higher internal misorientations in the FZ than in the HAZ due to interconnected walls between the δ-ferrite grains. However, the largest KAM values were observed in the BM between γ-austenite and the deformation-induced α’-martensite phases. X-ray diffraction revealed that the residual stresses in the cross-section of the welded seam were compression-type and then switched to tension-type in the outer surface. Full article

Upon infection, viruses hijack the cell machinery and remodel host cell structures to utilize them for viral proliferation. Since viruses are about a thousand times smaller than their host cells, imaging virus-host interactions at high spatial resolution is like looking for a needle in a haystack. Scouting gross cellular changes with fluorescent microscopy is only possible for well-established viruses, where fluorescent tagging is developed. Soft X-ray tomography (SXT) offers 3D imaging of entire cells without the need for chemical fixation or labeling. Here, we use full-rotation SXT to visualize entire human B cells infected by the herpes simplex virus 1 (HSV-1). We have mapped the temporospatial remodeling of cells during the infection and observed changes in cellular structures, such as the presence of cytoplasmic stress granules and multivesicular structures, formation of nuclear virus-induced dense bodies, and aggregates of capsids. Our results demonstrate the power of SXT imaging for scouting virus-induced changes in infected cells and understanding the orchestration of virus-host remodeling quantitatively. Full article

The Gram-positive and spore-forming bacterium Brevibacillus laterosporus (Bl) belongs to the Brevibacillus brevis phylogenetic cluster. Isolates of the species have demonstrated pesticidal potency against a wide range of invertebrate pests and plant diseases. Two New Zealand isolates, Bl 1821L and Bl 1951, are under development as biopesticides for control of diamondback moth and other pests. However, due to the often-restricted growth of these endemic isolates, production can be an issue. Based on the previous work, it was hypothesised that the putative phages might be involved. During investigations of the cause of the disrupted growth, electron micrographs of crude lysate of Bl 1821L showed the presence of phages’ tail-like structures. A soft agar overlay method with PEG 8000 precipitation was used to differentiate between the antagonistic activity of the putative phage and phage tail-like structures (bacteriocins). Assay tests authenticated the absence of putative phage activity. Using the same method, broad-spectrum antibacterial activity of Bl 1821L lysate against several Gram-positive bacteria was found. SDS-PAGE of sucrose density gradient purified and 10 kD MWCO concentrated lysate showed a prominent protein band of ~48 kD, and transmission electron microscopy revealed the presence of polysheath-like structures. N-terminal sequencing of the ~48 kD protein mapped to a gene with weak predicted amino acid homology to a Bacillus PBSX phage-like element xkdK, the translated product of which shared >90% amino acid similarity to the phage tail-sheath protein of another Bl published genome, LMG15441. Bioinformatic analysis also identified an xkdK homolog in the Bl 1951 genome. However, genome comparison of the region around the xkdK gene between Bl 1821L and Bl 1951 found differences including two glycine rich protein encoding genes which contain imperfect repeats (1700 bp) in Bl 1951, while a putative phage region resides in the analogous Bl 1821L region. Although comparative analysis of the genomic organisation of Bl 1821L and Bl 1951 PBSX-like region with the defective phages PBSX, PBSZ, and PBP 180 of Bacillus subtilis isolates 168 and W23, and Bacillus phage PBP180 revealed low amino acids similarity, the genes encode similar functional proteins in similar arrangements, including phage tail-sheath (XkdK), tail (XkdO), holin (XhlB), and N-acetylmuramoyl-l-alanine (XlyA). AMPA analysis identified a bactericidal stretch of 13 amino acids in the ~48 kD sequenced protein of Bl 1821L. Antagonistic activity of the purified ~48 kD phage tail-like protein in the assays differed remarkably from the crude lysate by causing a decrease of 34.2% in the number of viable cells of Bl 1951, 18 h after treatment as compared to the control. Overall, the identified inducible phage tail-like particle is likely to have implications for the in vitro growth of the insect pathogenic isolate Bl 1821L. Full article

The formation of pathological bone deposits within soft tissues, termed heterotopic ossification (HO), is common after trauma. However, the severity of HO formation varies substantially between individuals, from relatively isolated small bone islands through to extensive soft tissue replacement by bone giving rise to debilitating symptoms. The aim of this study was to identify novel candidate therapeutic molecular targets for severe HO. We conducted a genome-wide scan in men and women with HO of varying severity following hip replacement for osteoarthritis. HO severity was dichotomized as mild or severe, and association analysis was performed with adjustment for age and sex. We next confirmed expression of the gene encoded by the lead signal in human bone and in primary human mesenchymal stem cells. We then examined the effect of gene knockout in a murine model of osseous trans-differentiation, and finally we explored transcription factor phosphorylation in key pathways perturbed by the gene. Ten independent signals were suggestively associated with HO severity, with KIF26B as the lead. We subsequently confirmed KIF26B expression in human bone and upregulation upon BMP2-induced osteogenic differentiation in primary human mesenchymal stem cells, and also in a rat tendo-Achilles model of post-traumatic HO. CRISPR-Cas9 mediated knockout of Kif26b inhibited BMP2-induced Runx2, Sp7/Osterix, Col1A1, Alp, and Bglap/Osteocalcin expression and mineralized nodule formation in a murine myocyte model of osteogenic trans-differentiation. Finally, KIF26B deficiency inhibited ERK MAP kinase activation during osteogenesis, whilst augmenting p38 and SMAD 1/5/8 phosphorylation. Taken together, these data suggest a role for KIF26B in modulating the severity of post-traumatic HO and provide a potential novel avenue for therapeutic translation. Full article

Krabi and Nakhon Si Thammarat are two coastal provinces in Thailand facing substantial threats from climate change induced hydrometeorological hazards, including enhanced coastal erosion and flooding. Human populations and livelihoods in these coastal provinces are at greater risk than those in inland provinces. However, little is known about the communities’ resilience and coping capacities regarding hydrometeorological hazards of varying magnitudes. The study conducted a quantitative socio-economic assessment of how people in Krabi and Nakhon Si Thammarat provinces manage and respond to hydrometeorological hazards, examining their resilience and coping capacities. This was a cross-sectional study based on secondary data collection on the social and economic dimensions of resilience, and a review of literature on coping mechanisms to hydrometeorological hazards within the study area. Measuring and mapping socio-economic resilience was based on the available data gathered from the social and economic dimensions, with existing or standard indicators on exposure and vulnerability applied uniformly across subdistricts. A combination of social and economic dimensions produced novel socio-economic resilience index scores by subdistrict, which were mapped accordingly for the two coastal provinces. The study also derived a coping capacity index scores by combining availability of skills or soft capacity and availability of structural resources or hard coping capacity. Socio-economic resilience index scores varied greatly amongst subdistricts. Combining the soft and hard coping capacities, the average score across districts in both provinces was 3 out of a possible 4, meaning that most of the districts were largely resilient. However, variations also existed by subdistrict. Few subdistricts in both Krabi and Nakhon Si Thammarat provinces had low coping capacity index scores between 1 and 2 out of 4. District averages of socio-economic resilience scores mask the variations at subdistrict level. More studies with rigorous methodologies at village or neighborhood level is needed to obtain a nuanced understanding of community resilience to hydrometeorological hazards. Full article