Search Results (72)

The Slime Mould Algorithm (SMA) is a widely used swarm intelligence algorithm. Encouraged by the theory of no free lunch and the inherent shortcomings of the SMA, this work proposes a new variant of the SMA, called the BWSMA, in which three improvement mechanisms are integrated. The adaptive greedy mechanism is used to accelerate the convergence of the algorithm and avoid ineffective updates. The best–worst management strategy improves the quality of the population and increases its search capability. The stagnant replacement mechanism prevents the algorithm from falling into a local optimum by replacing stalled individuals. In order to verify the effectiveness of the proposed method, this paper conducts a full range of experiments on the CEC2018 test suite and the CEC2022 test suite and compares BWSMA with three derived algorithms, eight SMA variants, and eight other improved algorithms. The experimental results are analyzed using the Wilcoxon rank-sum test, the Friedman test, and the Nemenyi test. The results indicate that the BWSMA significantly outperforms these compared algorithms. In the comparison with the SMA variants, the BWSMA obtained average rankings of 1.414, 1.138, 1.069, and 1.414. In comparison with other improved algorithms, the BWSMA obtained average rankings of 2.583 and 1.833. Finally, the applicability of the BWSMA is further validated through two structural optimization problems. In conclusion, the proposed BWSMA is a promising algorithm with excellent search accuracy and robustness. Full article

Background: Long-lasting insecticidal nets (LLINs) have significantly reduced the malaria burden in recent decades, and this malaria prevalence reduction has been achieved through the upgrading of pyrethroid long-lasting insecticidal nets. However, this reduction has stalled due to many factors, including rapidly developing pyrethroid resistance. Method: The protocol was registered in PROSPERO, and we used Cochrane methodology to assess bias and evidence quality. Three reviewers extracted data from individual studies, and a meta-analysis was performed using Excel and STATA version 17, expressing the data as a risk ratio. Result: A study involving 21,916 households from 11 randomized controlled trials showed that the chlorfenapyr treatment group had a 10% reduction in malaria infection risk, with a pooled overall prevalence of 25.96 per 100 children in the chlorfenapyr group and 32.38 per 100 children in the piperonyl butoxide group, compared to 41.60 per 100 children in the control (pyrethroid-only) group. This meta-analysis determined that the entomological outcomes of effectiveness and efficacy showed that these treatments effectively reduced vector density per household per night and mean inoculation rates, with a 23% reduction in chlorfenapyr, a 7% reduction in pyrethroid-only treatments, and a 12% reduction in piperonyl-butoxide-treated groups. This study shows that chlorfenapyr (CFP) and pyriproxyfen (PPF) LLINs are highly effective and more efficacious in reducing malaria infection, case incidence, and anemia among children, as well as in reducing mean indoor vector density, mean entomological inoculation rate, and sporozoite rate, compared to pyriproxyfen (PPF) LLINs in Africa. Conclusions: This study found that chlorfenapyr (CFP) LLINs are highly effective and more efficacious in reducing malaria infection, case incidence, and anemia among children in Africa. Therefore, policymakers and health planners should place strong emphasis on addressing the effectiveness, efficacy, and resistance management of LLINs as part of their current public health agenda to eliminate malaria. Full article

Community-Engaged Research (CER) arose as a means of increasing the democratic participation of communities that study outcomes directly impact. CER has been identified as a recommended approach for conducting biomedical and behavioral health research with Black communities, a population that has been excluded from and exploited by academic health research for centuries. However, solely increasing community participation without identifying and redressing racialized power imbalances within community–academic partnerships involving Black populations can stall progress towards racial health equity. The purpose of this study was to explore how power can be redistributed equitably in community–academic health research partnerships involving Black populations. Utilizing the qualitative methodological approach of critical narrative inquiry, counter-stories from 12 Black individuals who have served as community partners on U.S.-based academic health research teams were collected via in-depth semi-structured narrative interviews. A reflexive thematic analysis approach was utilized to identify and analyze strategies expressed by study participants for increasing community agency, efficacy, and solidarity in health research. By centering the voices of Black community members who have directly engaged with academic health research institutions, this study sought to amplify the desires and aspirations of Black communities regarding shifting power in health research processes and outcomes. Full article

Background: Chemotherapy-induced peripheral neuropathy (CIPN) affects 20–85% of individuals exposed to neurotoxic chemotherapeutic agents. Perineural electrical dry needling (PEDN) and neural mobilization (NM) interventions may be beneficial in the management of chronic neurogenic pain; however, there is a paucity of research on the efficacy of both interventions for CIPN. Methods: Three patients were referred to an outpatient physical therapy clinic with chronic neuropathic pain associated with CIPN. Each underwent PEDN and NM twice weekly until goals were met or progress stalled. The primary outcome measure was the Numeric Pain Rating Scale (NPRS). Secondary outcomes included the Global Rating of Change (GROC) and the Lower Extremity Functional Scale (LEFS). All outcome measures were assessed at evaluation and discharge. Results: At discharge, patients A and B exceeded the minimum clinically important difference (MCID) for the primary and secondary outcome measures, indicating decreased neuropathic pain and improved lower extremity function. Patient C improved in all outcome measures but only experienced clinically meaningful changes in the NPRS and LEFS, not the GROC. Conclusions: Following 4–8 sessions of PEDN and NM, three patients with CIPN demonstrated clinically meaningful improvements in chronic lower extremity neuropathic pain and function. PEDN and NM may be beneficial in the management of patients presenting with chronic neuropathic pain secondary to CIPN. Full article

Polygain™ (PG), a polyphenolic extract from sugarcane, has recently been identified as a potential additive to reduce methane (CH₄) emissions in livestock. This experiment examined the effects of PG on the enteric CH₄ emission from Holstein Friesian weaned calves. Calves were allocated to annual pasture grazing and received supplementary pellets (200 g/calf/day; Barastoc calf-rearer cubes—Ridley Corporation). The experimental design followed was a completely randomized design (CRD), comprising 24 female calves (4–5 months old) allocated to two equal groups; control (standard pellets) vs. treatment (pellets formulated by adding PG to control pellets to deliver 10 g PG/calf/day). Experimental diets were fed for three months between August and November 2023, including a two-week adaptation period. Calves were weighed at the start and at the end of the study. A GreenFeed (C-Lock Pvt Ltd.) emission monitoring unit (GEM) was used to measure GHG emissions from the experimental calves in their groups in a 2-day rotational cycle. During a visit to the GEM, the calves were encouraged to enter an enclosed area or individual feeding stall where enteric CH₄, CO₂, O₂, H₂, and H₂S measurements were taken. The results indicated a significant effect of PG supplementation on enteric methane emission in calves, with a lower production of CH₄ in calves supplemented with PG (26.66 ± 2.06 g/day) as compared to the control group (35.28 ± 1.39 g/day, p < 0.001). The CO₂/O₂ ratio in the treatment (235 ± 14) and control groups (183 ± 9.6) differed significantly (p < 0.001). Overall, PG supplementation (10 g/calf/day) reduced their average methane emission per day and did not adversely affect the growth and development of experimental calves, confirming its useful anti-methanogenic potential. Full article

Foodborne diseases are a growing public health problem worldwide, and chemical foodborne disease outbreaks (FBDOs) often have serious consequences. This study aimed to explore the epidemiological characteristics of chemical FBDOs in Zhejiang Province, China, and propose targeted prevention and control measures. Descriptive statistical methods were used to analyze chemical FBDO data collected from the Foodborne Disease Outbreaks Surveillance System in Zhejiang Province from 2011 to 2023. From 2011 to 2023, 74 chemical FBDOs were reported in Zhejiang Province, resulting in 461 cases, 209 hospitalizations, and one death. In contrast to other types of FBDOs, the percentage of hospitalized cases in chemical FBDOs was the highest (45.34%) (chi-square = 1047.9, p < 0.001). Outbreaks caused by nitrite accounted for the largest percentage (56.76%), followed by lead (17.57%). Outbreaks caused by nitrite occurred mainly in households (27), followed by restaurants (6), street stalls (5), and work canteens (3). Among all nitrite-related outbreaks, 59.52% (25/42) were caused by cooking food where it was used as a common seasoning, 26.19% (11/42) by eating pickled vegetables, 7.14% (3/42) by eating cooked meat products, and 4.76% (2/42) by eating grain products. Outbreaks caused by the misuse of nitrite in cooking mainly occurred in households (68%, 17/25), street stalls (16%, 4/25), work canteens (8%, 2/25), and restaurants (8%, 2/25). Outbreaks caused by eating pickled vegetables occurred mainly in households (90.91%, 10/11), and one outbreak occurred in a work canteen. Outbreaks caused by lead (n = 13) occurred in households, and liquor was involved in 12 outbreaks where they were caused by residents consuming yellow rice wine stored in tin pots. In view of the frequent outbreaks of chemical foodborne diseases in our province from 2011 to 2023, a variety of prevention and control measures were proposed based on the research results of the temporal and regional distribution, food and food establishments involved, and the etiological agents of the chemical FBDOs. However, the effectiveness of these recommendations needs to be further verified and studied. In general, public health institutions should further strengthen the surveillance and health education of the population. Individuals should store toxic chemicals, such as nitrates, pesticides, and rodenticides correctly to avoid poisoning by ingestion. In view of the chemical FBDOs caused by food in the catering and distribution links, relevant departments should strengthen targeted supervision. Full article

This experiment determined the effects of two different starch sources when offered twice a day to cows during the early postpartum period (1 to 23 d postpartum, treatment period) on dry matter intake (DMI), feeding behavior, and milk production. The subsequent effects on milk production in the carryover period (24 to 72 d) where cows received a common diet (grazed perennial ryegrass pasture plus concentrate supplements) were also measured. Thirty-two multiparous dairy cows were offered concentrate feed (8 kg DM/d) containing 5 kg DM of crushed wheat grain or ground corn grain (7 h in vitro starch digestibility of 65.8% and 58.8%, respectively). At each milking (morning and afternoon), cows were offered half of the concentrate feed, and upon return to their individual stalls, they were offered perennial ryegrass pasture silage (56.2% neutral detergent fiber (NDF), 39.8% acid detergent fiber (ADF), 16.6% crude protein (CP)) at 130% of their expected daily intake. Dry matter intake, milk yield, and body weight were recorded daily. Blood and milk sampling, body condition score, and feeding behavior measurements were performed weekly during the treatment period. During the carryover period, milk sampling and body condition score measurements were conducted on a weekly and fortnightly basis, respectively. In the treatment and carryover periods, there was no significant effect of starch source treatment on DMI, milk yield, milk composition, change in body weight, or body condition. Similarly, the starch source did not affect the concentrations of blood markers of energy status or inflammatory response. Despite differences in the in vitro starch fermentability between treatments, the starch source did not significantly affect production responses. It is possible that the elevated NDF and ADF concentrations of the forage offered during the treatment period, the limited difference in starch fermentability between treatments, and the temporal supply of fuels to the liver when starch sources were offered twice a day may have offset the effects of the type of starch source on DMI and production via physical signals associated with rumen fill and distention. Full article

In populations of healthy show horses, the subclinical transmission and circulation of respiratory pathogens can lead to disease outbreaks. Due to recent outbreaks of equine herpesvirus-1 myeloencephalopathy (EHM) in the USA and Europe, many show organizers have instituted various biosecurity protocols such as individual horse testing, monitoring for early clinical disease and increasing hygiene and cleanliness protocols. The aim of this study was to determine the accuracy of detecting EHV-1 in the various environmental samples collected from the stalls of subclinical shedders. Four healthy adult horses were vaccinated intranasally with a modified-live EHV-1 vaccine in order to mimic subclinical shedding. Three additional horses served as non-vaccinated controls. All the horses were stabled in the same barn in individual stalls. Each vaccinated horse had nose-to-nose contact with at least one other horse. Prior to the vaccine administration, and daily thereafter for 10 days, various samples were collected, including a 6” rayon-tipped nasal swab, an environmental sponge, a cloth strip placed above the automatic waterer and an air sample. The various samples were processed for nucleic acid purification and analyzed for the presence of EHV-1 via quantitative PCR (qPCR). EHV-1 in nasal secretions was only detected in the vaccinated horses for 1–2 days post-vaccine administration. The environmental sponges tested EHV-1 qPCR-positive for 2–5 days (median 3.5 days) in the vaccinated horses and 1 day for a single control horse. EHV-1 was detected by qPCR in stall strips from three out of four vaccinated horses and from two out of three controls for only one day. EHV-1 qPCR-positive air samples were only detected in three out of four vaccinated horses for one single day. For the vaccinated horses, a total of 25% of the nasal swabs, 35% of the environmental stall sponges, 7.5% of the strips and 7.5% of the air samples tested qPCR positive for EHV-1 during the 10 study days. When monitoring the subclinical EHV-1 shedders, the collection and testing of the environmental sponges were able to detect EHV-1 in the environment with greater frequency as compared to nasal swabs, stationary strips and air samples. Full article

Inadequate management conditions can impair the welfare of captive-bred horses. Understanding individuals’ viewpoints and the factors influencing their decisions about adopting or avoiding certain practices may provide insights into their motivations and decision-making processes. This is particularly relevant in the equestrian community, where equine practitioners and enthusiasts often engage in harmful practices. We explored the beliefs, knowledge, and attitudes of equine practitioners and enthusiasts about horse welfare and the barriers that prevent them from employing better management practices that are essential to promoting horses’ welfare. The study consisted of in-depth semi-structured interviews conducted in person with 31 individuals directly involved in the equestrian environment in Brazil. Responses were analyzed through thematic analysis with a data-driven deductive approach. Participants’ beliefs, knowledge and attitudes to horse welfare were divided into three themes. The first theme, “Let the horse be a horse”, captured participants’ perceptions about how physical and mental aspects related to the nature and welfare of horses. The second theme, “Everyone does it like that”, includes the social norms that influence decisions about the practices that impact on the welfare of the horses. The third theme, “Beyond utopia: how and why horses are managed the way they are”, covered barriers that participants perceived as impediments to the use of best practices for the welfare of horses. While participants demonstrated awareness of welfare issues and acknowledged factors that negatively impact horses, there was a notable discrepancy between this knowledge and the implementation of improved management practices. This could be explained by several perceived barriers to implementing management practices that could enhance horse welfare, including lack of financial resources, limited physical space, shortage of qualified labor, time constraints, inadequate tools, and insufficient knowledge. Additionally, we identified deeply rooted social norms within the equestrian community and culturally established practices that limit approaches to horse welfare. Participants underscored the influence of these norms and different interpretations of “letting the horse be a horse” based on the horse’s value and purpose. Concerning low-value horses, the primary justifications for stall housing and concentrated feeding were linked to elevated costs involved in spatial demands and labor; in contrast, for high-value horses used in performance and aesthetics, the arguments shifted to potential benefits to the horses’ well-being. From an ethical perspective, ideally, individuals should refrain from owning horses if they cannot ensure the animals’ welfare. Additionally, if the equestrian community neglects public attitudes towards animal welfare, it risks eroding its social license. Full article

Cattle are frequent carriers of Campylobacter spp.; therefore, these bacteria may be transmitted to humans through meat or milk. Campylobacter spp. in raw milk derives most commonly from secondary fecal contamination during the milking process; however, the udder excretion of Campylobacter may be a cause of milk-borne infection. Studies were carried out on a Campylobacter-positive farm with two different housing systems (with free-stall and tie-stall systems). The sampling process comprised several stages, including samples being taken from animals, such as from raw milk and feces, and from the environment, such as the from floor in the milking parlor and from teat cups. None of the individual raw milk samples or swabs from the floor in the parlor before the milking process were positive for Campylobacter spp. Simultaneously, Campylobacter spp. was isolated from all swabs from the floor after the milking process and in the bulk tank milk samples from the two farms. The incidence of Campylobacter isolated from fecal and teat swab samples ranged from 15.4% to 26.7% and from 8.9% to 25%, respectively. Altogether, 59 recovered Campylobacter isolates were classified, based on sequencing of the flaA short variable region, showing 15 different allele types, and the majority of them were distributed among one farm. Analysis of the virulence and antimicrobial properties showed that genes related to adherence, invasion and cytotoxicity were widely distributed among the Campylobacter recovered strains. In relation to AMR, multidrug resistance was noted in 16.1% of strains. Full article

The aim of this study is to show the potential of a vision-based system using a single 360° camera to describe the dairy cows’ behaviour in a free-stall barn with an automatic milking system. A total of 2299 snapshots were manually evaluated, counting the number of animals that were lying, standing and eating. The average capture rate of animals in the picture is 93.1% (counted animals/actual numbers of animals). In addition to determining the daily lying, standing and eating times, it is also possible to allocate animals to the individual functional areas so that anomalies such as prolonged standing in the cubicle or lying in the walkway can be detected at an early stage. When establishing a camera monitoring system in the future, attention should be paid to sufficient resolution of the camera during the night as well as the reduction of the concealment problem by animals and barn equipment. The automatic monitoring of animal behaviour with the help of 360° cameras can be a promising innovation in the dairy barn. Full article

The first aim of this study was to calculate the prevalence of painful lesions of the digits (“alarm” lesions; ALs) in Swiss dairy herds and cow–calf operations over a three-year study period. The following ALs were included in the calculation: the M2 stage of digital dermatitis (DD M2), ulcers (U), white line fissures (WLF) of moderate and high severity, white line abscesses (WLA), interdigital phlegmon (IP) and swelling of the coronet and/or bulb (SW). Between February 2020 and February 2023, digit disorders were electronically recorded during routine trimmings by 40 specially trained hoof trimmers on Swiss cattle farms participating in the national claw health programme. The data set used consisted of over 35,000 observations from almost 25,000 cows from 702 herds. While at the herd-level, the predominant AL documented in 2022 was U with 50.3% followed by WLF with 38.1%, at the cow-level, in 2022, it was DD M2 with 5.4% followed by U with 3.7%. During the study period, within-herd prevalences of ALs ranged from 0.0% to a maximum of 66.1% in 2020. The second aim of this study was to determine herd- and cow-level risk factors associated with digital dermatitis (DD), U and white line disease (WL) in dairy cows using data from 2022. While for DD, analysed herd-level factors appeared to have a greater effect on the probability of its occurrence, the presence of U and WL was mainly associated with the analysed cow-level factors. The risk for DD increased with a higher herd trimming frequency. Herds kept in tie stalls had a lower risk for DD and WL and a higher risk for U compared to herds kept in loose housing systems. Herds with predominantly Holstein Friesian cows as well as Holstein Friesian cows had a higher risk for the occurrence of DD compared to herds and cows of other breeds. With increasing parity, cows had a higher risk of developing U and WL, whereas for DD, parity was negatively associated with prevalence. Cows trimmed during the grazing period had a higher risk of U and WL than cows trimmed during the housing period. These findings may contribute to improve management measures affecting the health of the digits in farms with structures similar to those evaluated in the current study, such as small herds with frequent access to pasture. Further research is warranted to demonstrate how measures addressing the current results combined with those of individual herd risk assessments might contribute to an improvement in the health of the digits in the respective dairy herds. Full article

PrimPol is a DNA primase/polymerase from the Archaeo-Eukaryotic Primase (AEP) superfamily that enables the progression of stalled replication forks by synthesizing DNA primers ahead of blocking lesions or abnormal structures in the ssDNA template. PrimPol’s active site is formed by three AEP-conserved motifs: A, B and C. Motifs A and C of human PrimPol (HsPrimPol) harbor the catalytic residues (Asp¹¹⁴, Glu¹¹⁶, Asp²⁸⁰) acting as metal ligands, whereas motif B includes highly conserved residues (Lys¹⁶⁵, Ser¹⁶⁷ and His¹⁶⁹), which are postulated to stabilize 3′ incoming deoxynucleotides (dNTPs). Additionally, other putative nucleotide ligands are situated close to motif C: Lys²⁹⁷, almost invariant in the whole AEP superfamily, and Lys³⁰⁰, specifically conserved in eukaryotic PrimPols. Here, we demonstrate that His¹⁶⁹ is absolutely essential for 3′dNTP binding and, hence, for both primase and polymerase activities of HsPrimPol, whereas Ser¹⁶⁷ and Lys²⁹⁷ are crucial for the dimer synthesis initiation step during priming, but dispensable for subsequent dNTP incorporation on growing primers. Conversely, the elimination of Lys¹⁶⁵ does not affect the overall primase function; however, it is required for damage avoidance via primer–template realignments. Finally, Lys³⁰⁰ is identified as an extra anchor residue to stabilize the 3′ incoming dNTP. Collectively, these results demonstrate that individual ligands modulate the stabilization of 3′ incoming dNTPs to optimize DNA primer synthesis efficiency during initiation and primer maturation. Full article

The aim of this study was to use environmental sampling to determine the frequency of detection of selected equine respiratory viruses and bacteria in horses attending a multi-week equestrian show during the winter months. At four time points during showing, environmental sponge samples were collected from all stalls on the property and tested for the presence of equine herpesvirus-1 (EHV-1), EHV-2, EHV-4, equine influenza virus (EIV), equine rhinitis B virus (ERBV), Streptococcus equi ss. equi (S. equi), and S. equi ss. zooepidemicus (S. zooepidemicus) using real-time PCR (PCR). Environmental sponges were collected from all 53 barns by using one sponge for up to 10 stalls. Further, 2/53 barns were randomly selected for individual stall sampling in order to compare the results between individual and pooled stall samples. A total of 333/948 (35.13%, 95% CI 32.09–38.26%) pooled environmental stall sponges tested PCR-positive for at least one of the selected respiratory pathogens. Streptococcus zooepidemicus was the most commonly detected pathogen in pooled samples (28.69%, 95% CI 25.83–31.69%), followed by EHV-2 (14.45%, 95% CI 12.27–16.85%), EHV-4 (1.37%, 95% CI 0.73–2.33%), and a very small percentage of pooled stall sponges tested PCR-positive for EHV-1, ERBV, EIV, and S. equi. In individual samples, 171/464 (36.85%, 95% CI 32.45–41.42%) environmental stall sponges tested PCR-positive for at least one of the selected pathogens, following a similar frequency of pathogen detection as pooled samples. The detection frequency of true respiratory pathogens from environmental samples was higher during the winter months compared to previous studies performed during spring and summer, and this testing highlights that such pathogens circulate with greater frequency during the colder months of the year. The strategy of monitoring environmental stall samples for respiratory pathogens circumvents the often labor-intensive collection of respiratory secretions from healthy horses and allows for a more efficient assessment of pathogen buildup over time. However, environmental stall testing for respiratory pathogens should not replace proper biosecurity protocols, but it should instead be considered as an additional tool to monitor the silent circulation of respiratory pathogens in at-risk horses. Full article

Equipping a farm with a milking parlor requires moving groups of cows from their pens to the part of the barn where milking takes place. The task of moving cows, carried out two or three times each day, shows links to the lying area of the barn. When the cows are taken from the pen to the milking parlor, some of them may be lying down, and this lying down must be interrupted. The forced standing up of cows can be considered in terms of their welfare. The aim of the study was to examine the number of cows lying in the stalls at the time of opening the exit gates in the pens in order to take the cows to the milking parlor. The study covered four pens, each with 12 cows. The behavior of the cows in the pens before morning and afternoon milking was recorded over 26 days. In the analysis, the dependent variable was the number of lying cows, and the independent variables were the time of milking and the level of sand in the lying stalls. The results of the study showed the significance of differences in the number of lying cows for stalls with a low and high level of sand, both in the case of morning and afternoon milking. Differences in the number of lying cows were also found when comparing the time before morning and afternoon milking. To compare the tendency of individual cows to lie down before going to milking, an index of forced standing up was proposed. The research conducted showed differences in the behavior of cows before leaving the pen to the milking parlor. The stage to reduce the forced standing up of cows is to equip the farm with an automatic milking system (AMS) instead of using a milking parlor. In barns with AMS, cows have full freedom to get up and approach the milking stall. The results of the observations are thus an additional argument confirming the benefits of using an automatic milking system, considered in terms of the welfare of dairy cows, regarding their lying down and getting up. Full article