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37 pages, 6314 KB  
Article
Cardiac Monitoring with Textile Capacitive Electrodes in Driving Applications: Characterization of Signal Quality and RR Duration Accuracy
by James Elber Duverger, Geordi-Gabriel Renaud Dumoulin, Victor Bellemin, Patricia Forcier, Justine Decaens, Ghyslain Gagnon and Alireza Saidi
Sensors 2025, 25(19), 6097; https://doi.org/10.3390/s25196097 - 3 Oct 2025
Abstract
Capacitive ECG sensors in automobiles enable unobtrusive heart rate monitoring as an indicator of a driver’s alertness and health. This paper introduces a capacitive sensor with textile electrodes and provides insights into signal quality and RR duration accuracy. Electrodes of various shapes, sizes, [...] Read more.
Capacitive ECG sensors in automobiles enable unobtrusive heart rate monitoring as an indicator of a driver’s alertness and health. This paper introduces a capacitive sensor with textile electrodes and provides insights into signal quality and RR duration accuracy. Electrodes of various shapes, sizes, and fabrics were integrated at various positions into the seat back of a driving simulator car seat. Seven subjects completed identical driving circuits with their cardiac signals being recorded simultaneously with textile electrodes and reference Ag-AgCl electrodes. Capacitive ECG signals with observable R peaks (after filtering) could be captured with almost all pairs of textile electrodes, independently of design or placement. Signal quality from textile electrodes was consistently lower compared with reference Ag-AgCl electrodes. Proximity to the heart or even contact with the body seems to be key but not enough to improve signal quality. However, accurate measurement of RR durations was mostly independent of signal quality since 90% of all RR durations measured on capacitive ECG signals had a percentage error below 5% compared to reference ECG signals. Accuracy was actually algorithm-dependent, where a classic Pan–Tompkins-based algorithm was interestingly outperformed by an in-house frequency-domain algorithm. Full article
(This article belongs to the Special Issue Smart Textile Sensors, Actuators, and Related Applications)
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11 pages, 285 KB  
Article
Diquark Study in Quark Model
by Xinmei Zhu, Hongxia Huang and Jialun Ping
Particles 2025, 8(4), 83; https://doi.org/10.3390/particles8040083 - 2 Oct 2025
Abstract
To investigate diquark correlation in baryons, the baryon spectra with different light–heavy quark combinations are calculated using Gaussian expansion method within both the naive quark model and the chiral quark model. By computing the diquark energies and separations between any two quarks in [...] Read more.
To investigate diquark correlation in baryons, the baryon spectra with different light–heavy quark combinations are calculated using Gaussian expansion method within both the naive quark model and the chiral quark model. By computing the diquark energies and separations between any two quarks in baryons, we analyze the diquark effect in the ud-q/Q, us-Q, ss-q/Q, and QQ-q/Q systems (where q=u,d, or s; Q=c,b). The results show that diquark correlations exist in baryons. In particular, for qq-Q and QQ-q systems, the same type of diquark exhibits nearly identical energy and size across different baryons. In the orbital ground states of baryons, scalar–isoscalar diquarks have lower energy and a smaller size compared to vector–isovector diquark, which qualifies them as “good diquarks”. In QQ-q systems, a larger mass of Q leads to a smaller diquark separation and a more pronounced diquark effect. In qq-Q systems, the separation between the two light quarks remains larger than that between a light and a heavy quark, indicating that the internal structure of such diquarks must be taken into account. A comparison between the naive quark model and the chiral quark model reveals that the introduction of meson exchange slightly increases the diquark size in most systems. Full article
(This article belongs to the Special Issue Strong QCD and Hadron Structure)
25 pages, 3762 KB  
Article
Cultural Ecosystem Services in Rural Areas: Assessing Demand and Supply for Ecologically Functional Areas (EFA)
by Malwina Michalik-Śnieżek, Halina Lipińska, Ilona Woźniak-Kostecka, Agnieszka Komor, Agnieszka Kępkowicz, Kamila Adamczyk-Mucha, Ewelina Krukow and Agnieszka Duniewicz
Sustainability 2025, 17(19), 8822; https://doi.org/10.3390/su17198822 - 1 Oct 2025
Abstract
Cultural ecosystem services (CES) play a key role in the sustainable development of rural areas—yet they remain poorly quantified in planning practice. This study examines the relationship between the supply and demand of CES provided by various types of Ecological Focus Areas (EFAs) [...] Read more.
Cultural ecosystem services (CES) play a key role in the sustainable development of rural areas—yet they remain poorly quantified in planning practice. This study examines the relationship between the supply and demand of CES provided by various types of Ecological Focus Areas (EFAs) in a rural landscape, using the municipality of Sosnowica (eastern Poland) as a case study. Landscapes such as forests, agricultural land, wetlands, and inland waters were evaluated using a set of biophysical and socio-economic indicators that reflect both their potential (supply) and actual use (demand) in terms of services such as recreation, landscape aesthetics, and cultural heritage. The findings reveal significant spatial disparities between CES supply and demand: forests and inland waters exhibit the highest supply potential, while agricultural land shows untapped opportunities in tourism and recreation. Wetlands, in particular, face notable service deficits—highlighting the need for targeted infrastructure and management interventions. Statistical analyses (Pearson correlation, Kruskal–Wallis test, Tukey HSD test) confirmed that the key factors shaping CES are accessibility and environmental attractiveness. The results indicate that CES mapping is a valuable tool for supporting sustainable rural planning, reinforcing local identity, counteracting depopulation, and stimulating socio-economic development. Full article
(This article belongs to the Section Health, Well-Being and Sustainability)
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25 pages, 5314 KB  
Article
Experimental Study on Bidirectional Bending Performance of Steel-Ribbed Composite Slabs for Electrical Substations
by Lin Li, Zhenzhong Wei, Yong Liu, Yunan Jiang, Haomiao Chen, Yu Zhang, Kaifa Zhang, Kunjie Rong and Li Tian
Buildings 2025, 15(19), 3540; https://doi.org/10.3390/buildings15193540 - 1 Oct 2025
Abstract
This study investigates the bidirectional bending performance of double- and triple-spliced steel-ribbed composite slabs for substation applications. Full-scale experiments and numerical parametric analyses were conducted to evaluate ultimate load, ductility, stiffness, failure modes, and load-transfer mechanisms. Results indicate that double-spliced slabs exhibit better [...] Read more.
This study investigates the bidirectional bending performance of double- and triple-spliced steel-ribbed composite slabs for substation applications. Full-scale experiments and numerical parametric analyses were conducted to evaluate ultimate load, ductility, stiffness, failure modes, and load-transfer mechanisms. Results indicate that double-spliced slabs exhibit better performance than triple-spliced slabs, showing a 24.5% higher ultimate load and 65.3% greater ductility, with well-developed orthogonal cracks and yielding of both longitudinal prestressing steel and transverse reinforcement. Triple-spliced slabs display partial bidirectional behavior due to reduced transverse integrity, with stresses in edge slabs concentrated at the corners. Compared with monolithic slabs, spliced slabs show nearly identical stiffness at cracking onset but progressively reduced stiffness, load capacity, and ductility in the mid-to-late loading stages. Joint-crossing reinforcement is critical for transverse load transfer, and increasing its diameter is more effective than increasing its strength in preventing premature joint-controlled failure. These findings provide significant theoretical guidance and technical support for the prefabricated construction of high-voltage substation floor systems. Full article
(This article belongs to the Section Building Structures)
17 pages, 360 KB  
Article
Beyond Satisfaction: Authenticity, Attachment, and Engagement in Shaping Revisit Intention of Palace Museum Visitors
by Qinzheng Fang and Wonkee Ko
Sustainability 2025, 17(19), 8803; https://doi.org/10.3390/su17198803 - 30 Sep 2025
Abstract
Cultural heritage sites play a crucial role in safeguarding identity, fostering cultural exchange, and generating sustainable tourism. Within this context, the Palace Museum in Beijing, which attracts 19 million annual visitors, offers a compelling case for examining the dynamics that shape revisit intention. [...] Read more.
Cultural heritage sites play a crucial role in safeguarding identity, fostering cultural exchange, and generating sustainable tourism. Within this context, the Palace Museum in Beijing, which attracts 19 million annual visitors, offers a compelling case for examining the dynamics that shape revisit intention. This study explores the relationships among perceived authenticity, place attachment, destination satisfaction, visitor engagement, and revisit intention within the context of heritage tourism. Using Partial Least Squares–Structural Equation Modeling (PLS-SEM), data were collected from local visitors to the Palace Museum to analyze both the direct and mediating effects of these constructs. Findings indicate that perceived authenticity significantly enhances both destination satisfaction and visitor engagement, while place attachment makes a strong contribution to visitor engagement. Moreover, visitor engagement emerged as a more influential mediator than destination satisfaction in linking perceived authenticity to revisit intention, showing the importance of immersive and meaningful participation in shaping tourists’ behavioral intentions. These results suggest that while satisfaction remains a relevant concept, strategies that emphasize authenticity-driven experiences and fostering of deeper emotional and participatory bonds are more effective in sustaining revisits. This study advances the understanding of heritage tourism and provides practical insights for managing iconic heritage sites such as the Palace Museum. Full article
26 pages, 7238 KB  
Article
Mutual Effects of Face-Swap Deepfakes and Digital Watermarking—A Region-Aware Study
by Tomasz Walczyna and Zbigniew Piotrowski
Sensors 2025, 25(19), 6015; https://doi.org/10.3390/s25196015 - 30 Sep 2025
Abstract
Face swapping is commonly assumed to act locally on the face region, which motivates placing watermarks away from the face to preserve the integrity of the face. We demonstrate that this assumption is violated in practice. Using a region-aware protocol with tunable-strength visible [...] Read more.
Face swapping is commonly assumed to act locally on the face region, which motivates placing watermarks away from the face to preserve the integrity of the face. We demonstrate that this assumption is violated in practice. Using a region-aware protocol with tunable-strength visible and invisible watermarks and six face-swap families, we quantify both identity transfer and watermark retention on the VGGFace2 dataset. First, edits are non-local—generators alter background statistics and degrade watermarks even far from the face, as measured by background-only PSNR and Pearson correlation relative to a locality-preserving baseline. Second, dependencies between watermark strength, identity transfer, and retention are non-monotonic and architecture-dependent. Methods that better confine edits to the face—typically those employing segmentation-weighted objectives—preserve background signal more reliably than globally trained GAN pipelines. At comparable perceptual distortion, invisible marks tuned to the background retain higher correlation with the background than visible overlays. These findings indicate that classical robustness tests are insufficient alone—watermark evaluation should report region-wise metrics and be strength- and architecture-aware. Full article
(This article belongs to the Special Issue Digital Image Processing and Sensing Technologies—Second Edition)
17 pages, 2347 KB  
Article
A Convolutional Neural Network-Based Vehicle Security Enhancement Model: A South African Case Study
by Thapelo Samuel Matlala, Michael Moeti, Khuliso Sigama and Relebogile Langa
Appl. Sci. 2025, 15(19), 10584; https://doi.org/10.3390/app151910584 - 30 Sep 2025
Abstract
This paper applies a Convolutional Neural Network (CNN)-based vehicle security enhancement model, with a specific focus on the South African context. While conventional security systems, including immobilizers, alarms, steering locks, and GPS trackers, provide a baseline level of protection, they are increasingly being [...] Read more.
This paper applies a Convolutional Neural Network (CNN)-based vehicle security enhancement model, with a specific focus on the South African context. While conventional security systems, including immobilizers, alarms, steering locks, and GPS trackers, provide a baseline level of protection, they are increasingly being circumvented by technologically adept adversaries. These limitations have spurred the development of advanced security solutions leveraging artificial intelligence (AI), with a particular emphasis on computer vision and deep learning techniques. This paper presents a CNN-based Vehicle Security Enhancement Model (CNN-based VSEM) that integrates facial recognition with GSM and GPS technologies to provide a robust, real-time security solution in South Africa. This study contributes a novel integration of CNN-based authentication with GSM and GPS tracking in the South African context, validated on a functional prototype.The prototype, developed on a Raspberry Pi 4 platform, was validated through practical demonstrations and user evaluations. The system achieved an average recognition accuracy of 85.9%, with some identities reaching 100% classification accuracy. While misclassifications led to an estimated False Acceptance Rate (FAR) of ~5% and False Rejection Rate (FRR) of ~12%, the model consistently enabled secure authentication. Preliminary latency tests indicated a decision time of approximately 1.8 s from image capture to ignition authorization. These results, together with positive user feedback, confirm the model’s feasibility and reliability. This integrated approach presents a promising advancement in intelligent vehicle security for regions with high rates of vehicle theft. Future enhancements will explore the incorporation of 3D sensing, infrared imaging, and facial recognition capable of handling variations in facial appearance. Additionally, the model is designed to detect authorized users, identify suspicious behaviour in the vicinity of the vehicle, and provide an added layer of protection against unauthorized access. Full article
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25 pages, 2161 KB  
Article
Long-Term Physical Activity Modulates Lipid Metabolism and Gene Expression in Muscle and Fat Tissues of Alentejano Pigs
by José Manuel Martins, André Albuquerque, David Silva, José A. Neves, Rui Charneca and Amadeu Freitas
Agriculture 2025, 15(19), 2047; https://doi.org/10.3390/agriculture15192047 - 29 Sep 2025
Abstract
This study examined the effect of long-term physical activity during the finishing period on meat and fat quality, and metabolic gene expression in obese Alentejano (AL) pigs. From 87.3 to 161.6 kg BW and for 130 days, eighteen pigs were assigned to either [...] Read more.
This study examined the effect of long-term physical activity during the finishing period on meat and fat quality, and metabolic gene expression in obese Alentejano (AL) pigs. From 87.3 to 161.6 kg BW and for 130 days, eighteen pigs were assigned to either individual pens without an exercise area (NE, n = 9) or an outdoor park with an exercise area (WE, n = 9). Both groups received identical commercial diets at 85% ad libitum intake. Loin (Longissimus lumborum—LL), tenderloin (Psoas major—PM), and dorsal subcutaneous fat samples were obtained at slaughter, and analyzed for fatty acid composition and gene expression. Physical activity modulated the fatty acid profile and key metabolic genes in muscle and fat tissues. WE pigs showed higher palmitoleic (p = 0.031) and linolenic (p = 0.022) acids in LL, while Fatty acid synthase and Leptin in LL were downregulated (p = 0.071 and p = 0.018, respectively); Fatty acid binding protein 4 was downregulated (p = 0.003) and Stearoyl-CoA desaturase upregulated (p = 0.020) in the PM of WE pigs, indicating changes in lipid metabolism. Also, Myosin heavy chain 7 was upregulated (p = 0.016) in LL, suggesting oxidative muscle remodeling. These findings suggest that moderate, long-term physical activity during finishing induces modest but favorable metabolic adaptations in muscle and fat tissues without compromising meat quality in AL pigs, supporting its use in traditional rearing systems aimed at balancing animal welfare and product quality in local breeds. Full article
(This article belongs to the Section Farm Animal Production)
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24 pages, 1734 KB  
Article
Genome Size Variation Is Associated with Hybrid Vigor in Near-Isogenic Backgrounds in Brassica napus
by Rui Wang, Meicui Yang, Haoran Shi, Yun Li, Jin Yang, Wanzhuo Gong, Qiong Zou, Lanrong Tao, Qiaobo Wu, Qin Yu, Hailan Liu and Shaohong Fu
Plants 2025, 14(19), 3013; https://doi.org/10.3390/plants14193013 - 29 Sep 2025
Abstract
Although heterosis plays a crucial role in enhancing crop yield and stress resistance, its underlying genetic mechanism remains not yet fully understood. Previous studies have shown that heterosis tends to increase with greater genetic distance in the absence of reproductive isolation barriers. However, [...] Read more.
Although heterosis plays a crucial role in enhancing crop yield and stress resistance, its underlying genetic mechanism remains not yet fully understood. Previous studies have shown that heterosis tends to increase with greater genetic distance in the absence of reproductive isolation barriers. However, whether variation in parental genome size alone can generate heterosis under near-isogenic backgrounds has not been thoroughly explored. Here, we used a rapeseed double haploid (DH) inducer line to generate progeny from the Pol CMS three-line hybrid Rongyou 18 (RY18). Although the progeny maintained the same ploidy level as the parents, their genome sizes showed notable variation (818.99–1024.88 Mb). To eliminate genetic distance effects, multiple DH progeny carrying restorer genes were crossed as paternal parents with the female parent 0068A of RY18, creating novel F1 hybrids. Using RY18 as the control, we observed a marked reduction in the genetic distance between the newly induced restorer line and the female parent (0068A). Correlation analysis further revealed a significant negative correlation (r = −0.310 *) between the paternal genome size and heterosis for thousand-seed weight (TSW). Furthermore, the genomic expansion in hybrid offspring relative to the male parent showed that significant correlations were observed between paternal genome size and heterosis over the standard for both TSW (r = 0.300, p < 0.05) and plot yield (r = 0.326, p < 0.05). Resequencing of high-and low-yielding F1 hybrids identified SNP sites, indicating that under an identical genetic background, heterosis for yield was more pronounced on chromosome A and chromosome C04. The doubled haploid (DH) induction line facilitates the generation of parental lines with distinct genome sizes, potentially providing a potential novel approach for studying heterosis research in Brassica napus. Full article
(This article belongs to the Special Issue Genetic Diversity and Population Structure of Plants)
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20 pages, 7927 KB  
Article
Achieving High-Quality Formed Hastelloy X Cladding Layers on Heterological 50CrVA Surface by Optimizing Process Parameters in Directed Energy Deposition
by Liming Xia, Hongqin Lei, Enjie Dong, Tingyu Chang, Linjie Zhao, Mingjun Chen, Junwen Lu and Jian Cheng
Micromachines 2025, 16(10), 1110; https://doi.org/10.3390/mi16101110 - 29 Sep 2025
Abstract
Hastelloy X exhibits outstanding thermal fatigue resistance, making it a promising material for repairing 50CrVA landing gear via directed energy deposition (DED). However, the substantial differences in composition and thermophysical properties between 50CrVA and Hastelloy X pose challenges by affecting interfacial microstructure and [...] Read more.
Hastelloy X exhibits outstanding thermal fatigue resistance, making it a promising material for repairing 50CrVA landing gear via directed energy deposition (DED). However, the substantial differences in composition and thermophysical properties between 50CrVA and Hastelloy X pose challenges by affecting interfacial microstructure and surface quality. This study investigates the effect of DED process parameters (laser power p, powder feed rate f, scanning speed v, and overlap rate) on the dilution ratio (η), microscopic morphology, surface flatness (ζ), and porosity of Hastelloy X claddings on a 50CrVA substrate. An optimization methodology integrating thermal–flow coupled simulation models and orthogonal experiments is developed to fabricate high-quality claddings. Furthermore, the corrosion–wear performance of the claddings is evaluated. The results indicate that the η of a single track increases with higher p or lower f, while it first increases and then decreases with the increase in v. Ablation marks tend to occur at excessive p or insufficient f, while low v causes surface ripples. The ζ of a single layer initially improves and subsequently deteriorates with increasing overlap rate. Porosity is significantly influenced by p and f. The optimal p, f, v, and overlap rate are 1600 W, 2.4 g/min, 240 mm/min, and 55%, respectively. The wear resistance of the cladding is nearly identical to that of the substrate, while corrosion resistance is significantly improved. This work provides a theoretical foundation for high-performance repair of 50CrVA landing gear in aircraft. Full article
(This article belongs to the Special Issue Advances in Digital Manufacturing and Nano Fabrication)
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22 pages, 1797 KB  
Article
A Novel Hybrid Deep Learning–Probabilistic Framework for Real-Time Crash Detection from Monocular Traffic Video
by Reşat Buğra Erkartal and Atınç Yılmaz
Appl. Sci. 2025, 15(19), 10523; https://doi.org/10.3390/app151910523 - 29 Sep 2025
Abstract
The rapid evolution of autonomous vehicle technologies has amplified the need for crash detection that operates robustly under complex traffic conditions with minimal latency. We propose a hybrid temporal hierarchy that augments a Region-based Convolutional Neural Network (R-CNN) with an adaptive time-variant Kalman [...] Read more.
The rapid evolution of autonomous vehicle technologies has amplified the need for crash detection that operates robustly under complex traffic conditions with minimal latency. We propose a hybrid temporal hierarchy that augments a Region-based Convolutional Neural Network (R-CNN) with an adaptive time-variant Kalman filter (with total-variation prior), a Hidden Markov Model (HMM) for state stabilization, and a lightweight Artificial Neural Network (ANN) for learned temporal refinement, enabling real-time crash detection from monocular video. Evaluated on simulated traffic in CARLA and real-world driving in Istanbul, the full temporal stack achieves the best precision–recall balance, yielding 83.47% F1 offline and 82.57% in real time (corresponding to 94.5% and 91.2% detection accuracy, respectively). Ablations are consistent and interpretable: removing the HMM reduces F1 by 1.85–2.16 percentage points (pp), whereas removing the ANN has a larger impact of 2.94–4.58 pp, indicating that the ANN provides the largest marginal gains—especially under real-time constraints. The transition from offline to real time incurs a modest overall loss (−0.90 pp F1), driven more by recall than precision. Compared to strong single-frame baselines, YOLOv10 attains 82.16% F1 and a real-time Transformer detector reaches 82.41% F1, while our full temporal stack remains slightly ahead in real time and offers a more favorable precision–recall trade-off. Notably, integrating the ANN into the HMM-based pipeline improves accuracy by 2.2%, while the time-variant Kalman configuration reduces detection lag by approximately 0.5 s—an improvement that directly addresses the human reaction time gap. Under identical conditions, the best RCNN-based configuration yields AP@0.50 ≈ 0.79 with an end-to-end latency of 119 ± 21 ms per frame (~8–9 FPS). Overall, coupling deep learning with probabilistic reasoning yields additive temporal benefits and advances deployable, camera-only crash detection that is cost-efficient and scalable for intelligent transportation systems. Full article
(This article belongs to the Section Computing and Artificial Intelligence)
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22 pages, 5662 KB  
Article
Physical Vapor Deposited TiN and TiAlN on Biomedical β-Type Ti-29Nb-13Ta-4.6Zr: Microstructural Characteristics, Surface Hardness Enhancement, and Antibacterial Activity
by Hakan Yilmazer
Coatings 2025, 15(10), 1126; https://doi.org/10.3390/coatings15101126 - 29 Sep 2025
Abstract
Beta (β)-type Ti-29Nb-13Ta-4.6Zr (TNTZ) alloys combine low modulus with biocompatibility but require improved surface properties for long-term implantation. This study aimed to enhance the surface mechanical strength and antibacterial performance of TNTZ by applying TiN and TiAlN coatings via PVD. Notably, TiAlN was [...] Read more.
Beta (β)-type Ti-29Nb-13Ta-4.6Zr (TNTZ) alloys combine low modulus with biocompatibility but require improved surface properties for long-term implantation. This study aimed to enhance the surface mechanical strength and antibacterial performance of TNTZ by applying TiN and TiAlN coatings via PVD. Notably, TiAlN was deposited on TNTZ for the first time, enabling a direct side-by-side comparison with TiN under identical deposition conditions. Dense TiN (~1.06 μm) and TiAlN (~1.73 μm) coatings were deposited onto solution-treated TNTZ and characterized by X-ray diffraction, scanning probe microscopy, Vickers microhardness, Rockwell indentation test (VDI 3198), static water contact angle measurements, and a Kirby–Bauer disk-diffusion antibacterial assay against Escherichia coli (E. coli). Both coatings formed face-centered cubic (FCC) structures with smooth interfaces (Ra ≤ 5.3 nm) while preserving the single-phase β matrix of the substrate. The hardness increased from 192 HV (uncoated) to 1059 HV (TiN) and 1468 HV (TiAlN), and the adhesion quality was rated as HF2 and HF1, respectively. The surface wettability changed from hydrophilic (48°) to moderately hydrophobic (82°) with TiN and highly hydrophobic (103°) with TiAlN. Similarly, the diameter of the no-growth zones increased to 18.02 mm (TiN) and 19.09 mm (TiAlN) compared to 17.65 mm for uncoated TNTZ. The findings indicate that TiAlN, in particular, provided improved hardness, adhesion, and hydrophobicity. Preliminary bacteriostatic screening under diffusion conditions suggested a modest relative antibacterial response, though the effect was not statistically significant between coated and uncoated TNTZ. Statistical analysis confirmed no significant difference between the groups (p > 0.05), indicating that only a preliminary bacteriostatic trend— rather than a definitive antibacterial effect—was observed. Both nitride coatings strengthened TNTZ without compromising its structural integrity, making TiAlN-coated TNTZ a promising candidate for next-generation orthopedic implants. Full article
(This article belongs to the Special Issue Films and Coatings with Biomedical Applications)
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19 pages, 5313 KB  
Article
Gibberellin Disrupts Hormonal Homeostasis and Anther Integrity to Trigger Sex Reversal in Spinach
by Tengqi Wang, Ehsan Khalid, Haoming Mao, Yihan Tong, Xinyu Xue, Yuru Tang, Lingmin Cai and Ray Ming
Int. J. Mol. Sci. 2025, 26(19), 9505; https://doi.org/10.3390/ijms26199505 - 28 Sep 2025
Abstract
Spinach is a dioecious vegetable and an excellent model for investigating plant sex differentiation. Exogenous gibberellin treatment induced sepal hypoplasia and sex reversal, converting 42% of stamens into pistils in male plants. Transcriptome analysis identified 112 male-biased genes enriched in stamen and pollen [...] Read more.
Spinach is a dioecious vegetable and an excellent model for investigating plant sex differentiation. Exogenous gibberellin treatment induced sepal hypoplasia and sex reversal, converting 42% of stamens into pistils in male plants. Transcriptome analysis identified 112 male-biased genes enriched in stamen and pollen development, while hormone profiling revealed coordinated changes in GA, cytokinins, auxin, jasmonic acid, and abscisic acid. Functional assays demonstrated that silencing SpAMS or SpPGIP caused extensive carpelization, and in situ hybridization localized their expression to developing anthers. Dual-luciferase assays confirmed that SpAMS directly activates the B-class gene SpPI, and genomic mapping placed SpAMS in the pseudo-autosomal region of the Y chromosome. These results indicate that GA disrupts hormonal homeostasis and anther wall integrity, while the SpAMS–SpPI pathway regulates tapetal development to maintain male identity. Our findings identify SpAMS as a key male-promoting factor in spinach and provide a framework for elucidating sex determination mechanisms in dioecious plants. Full article
(This article belongs to the Section Molecular Plant Sciences)
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19 pages, 289 KB  
Article
Self-Image and Mutual Perception of the Catholic and Evangelical Church of the Augsburg Confession in Upper Hungary in the Context of the Second Confessionalization
by Peter Šoltés
Religions 2025, 16(10), 1244; https://doi.org/10.3390/rel16101244 - 28 Sep 2025
Abstract
This study analyzes confessionally conditioned self-image and mutual perception of the Catholic and Evangelical Church of the Augsburg Confession in Upper Hungary in the context of the second confessionalization process. Based on comparative research of the contemporary press, including either the printed or [...] Read more.
This study analyzes confessionally conditioned self-image and mutual perception of the Catholic and Evangelical Church of the Augsburg Confession in Upper Hungary in the context of the second confessionalization process. Based on comparative research of the contemporary press, including either the printed or handwritten homiletic and catechetical literature, predominantly from the area of Upper Hungary, the study examines which phenomena and processes taking place since the 1830s until the end of the 1850s signaled a renewal in confessional identities in both the Catholic and Evangelical Churches. A particular emphasis has been placed on indicators of the second confessionalization, such as the debate on mixed marriages, a rising number of conversions, or legislative interventions in the freedom of religion. Through discourse analysis, the study explores how the image and self-image of the Catholic and Evangelical Church of the Augsburg Confession evolved as a result of the expansion of the catechetical literature and apologetic works and identifies the narrative strategies employed in their respective confessional discourses. The Catholic discourse stressed maintaining dogmatic integrity and Church authority in particular, whereas the Evangelicals more frequently accentuated a thorough biblicality and rationality as a counterposition to Catholic piety. Both traditions claimed exclusive access to “true religion” and used apologetic genres to defend and enhance their identity. Polemical texts also served as tools to form confessionally conditioned collective consciousness, as well as a part of contemporary cultural and political debates. Full article
15 pages, 2459 KB  
Article
The Effect of Liposomal DMU-212 on the Differentiation of Human Ovarian Granulosa Cells in a Primary 3D Culture Model
by Małgorzata Jόzkowiak, Dariusz Wawrzyniak, Alicja Kawczyńska, Paulina Skupin-Mrugalska, Mikołaj Czajkowski, Paul Mozdziak, Marta Podralska, Marek Żywicki, Bartosz Kempisty, Robert Z. Spaczyński and Hanna Piotrowska-Kempisty
Pharmaceuticals 2025, 18(10), 1460; https://doi.org/10.3390/ph18101460 - 28 Sep 2025
Abstract
Background/Objectives: Human ovarian granulosa cells (hGCs) are crucial to ovarian follicle development and function, exhibiting multipotency and the ability to differentiate into neuronal cells, chondrocytes, and osteoblasts in vitro. 3,4,5,4′-tetramethoxystilbene (DMU-212) is a methylated derivative of resveratrol, a natural polyphenol found in grapes [...] Read more.
Background/Objectives: Human ovarian granulosa cells (hGCs) are crucial to ovarian follicle development and function, exhibiting multipotency and the ability to differentiate into neuronal cells, chondrocytes, and osteoblasts in vitro. 3,4,5,4′-tetramethoxystilbene (DMU-212) is a methylated derivative of resveratrol, a natural polyphenol found in grapes and berries, with a wide spectrum of biological activities, including notable anticancer properties. Interestingly, DMU-212 exhibits cytotoxic effects predominantly on cancer cells while sparing non-cancerous ones, and evidence suggests that similar to resveratrol, it may also promote hGC differentiation. This study aimed to investigate the effects of the liposomal formulation of this methylated resveratrol analog—lipDMU-212—on the osteogenic differentiation ability of hGCs in a primary three-dimensional cell culture model. Methods: lipDMU-212 was formulated using the thin-film hydration method. GC spheroids’ viability was evaluated after exposure to lipDMU-212, an osteoinductive medium, or both. Osteogenic differentiation was confirmed using Alizarin Red staining and quantified by measuring Alkaline Phosphatase (ALP) activity on days 1, 7, and 15. RNA sequencing (RNA-seq) was performed to explore molecular mechanisms underlying lipDMU-212-induced differentiation. Results: lipDMU-212 promoted osteogenic differentiation of hGCs in the 3D cell culture model, as evidenced by increased mineralization and a ~4-fold increase in ALP activity compared with the control. RNA-seq revealed up-regulation of genes related to cell differentiation and cellular identity. Furthermore, JUN (+2.82, p = 0.003), LRP1 (+2.06, p = 0.05), AXIN1 (+3.02, p = 0.03), and FYN (+3.30, p = 0.01) were up-regulated, indicating modulation of the Wnt/β-catenin signaling pathway, a key regulator of osteoblast differentiation. Conclusions: The ability of GCs to differentiate into diverse tissue-specific cell types underscores their potential in regenerative medicine. This study contributes to the understanding of lipDMU-212’s role in osteogenic differentiation and highlights its potential in developing future therapies for degenerative bone diseases. Full article
(This article belongs to the Section Pharmacology)
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