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Keywords = hybrid position/force control

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19 pages, 2092 KB  
Article
A Hybrid Control Scheme for Backdriving a Surgical Robot About a Pivot Point
by Mehmet İsmet Can Dede, Emir Mobedi and Mehmet Fırat Deniz
Robotics 2025, 14(10), 144; https://doi.org/10.3390/robotics14100144 - 16 Oct 2025
Abstract
An incision point acts as the pivot point when a minimally invasive surgery procedure is applied. The assistive robot arms employed for such operation must have the capability to perform a remote center of motion (RCM) at this pivot point. Other than designing [...] Read more.
An incision point acts as the pivot point when a minimally invasive surgery procedure is applied. The assistive robot arms employed for such operation must have the capability to perform a remote center of motion (RCM) at this pivot point. Other than designing RCM mechanisms, a common practice is to use a readily available spatial serial robot arm and control it to impose this RCM constraint. When this assistive robot is required to be backdriven by the surgeon, the relation between the interaction forces/moments and the motion with RCM constraint becomes challenging. This paper carefully formulates a hybrid position/force control scheme for this relationship when any readily available robot arm that is coupled with a force/torque sensor is used for an RCM task. The verification of the formulation is carried out on a readily available robot arm by implementing the additional constraints that are derived from a surgical robot application. Full article
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29 pages, 6907 KB  
Article
Force-Closure-Based Weighted Hybrid Force/Position Fuzzy Coordination Control for Dual-Arm Robots
by Jun Dai, Yi Zhang and Weiqiang Dou
Actuators 2025, 14(10), 471; https://doi.org/10.3390/act14100471 - 26 Sep 2025
Viewed by 228
Abstract
There is a strong coupling between two arms in cooperative operations of dual-arm robots. To enhance the coordination and cooperation ability of dual-arm robots, a force-closure-based weighted hybrid force/position fuzzy coordination control method is proposed. Firstly, to improve the grasping stability of dual-arm [...] Read more.
There is a strong coupling between two arms in cooperative operations of dual-arm robots. To enhance the coordination and cooperation ability of dual-arm robots, a force-closure-based weighted hybrid force/position fuzzy coordination control method is proposed. Firstly, to improve the grasping stability of dual-arm robots, the force-closure dynamic constraints are established by combining the friction cone constraints with the force and torque balance constraints. Then the optimal distribution of contact force is performed according to the minimum energy consumption principle. Secondly, to enhance the coordination of dual-arm robots, the weighted hybrid force/position control method is modified by adding the synchronization error between two arms. Then the Lyapunov method is adopted to prove the stability of the proposed coordination control method. Thirdly, the fuzzy self-tuning technique is adopted to adjust the control gains automatically. Lastly, a simulation and experiment are performed for collaborative transport. The results show that, compared with the position coordination control and the traditional hybrid force/position control, the weighted hybrid force/position fuzzy coordination control can improve control accuracy and has good cooperation ability and strong robustness. Therefore, the proposed method can effectively realize the coordination control of dual-arm robots. Full article
(This article belongs to the Section Actuators for Robotics)
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30 pages, 10255 KB  
Article
Hybrid Design Optimization Methodology for Electromechanical Linear Actuators in Automotive LED Headlights
by Mario Đurić, Luka Selak and Drago Bračun
Actuators 2025, 14(10), 465; https://doi.org/10.3390/act14100465 - 24 Sep 2025
Viewed by 409
Abstract
The development of electromechanical linear actuators (EMLAs) aims at compactness, energy efficiency, and high reliability. Conventional design methods often rely on costly prototypes and individual considerations of mechanics, electromagnetics, and control dynamics. This leads to long development cycles, inadequate treatment of nonlinear effects, [...] Read more.
The development of electromechanical linear actuators (EMLAs) aims at compactness, energy efficiency, and high reliability. Conventional design methods often rely on costly prototypes and individual considerations of mechanics, electromagnetics, and control dynamics. This leads to long development cycles, inadequate treatment of nonlinear effects, and suboptimal performance. To address these challenges, our paper introduces a novel hybrid design methodology, integrating Analytical Modeling, Finite Element Analysis (FEA), Genetic Algorithms (GAs), and targeted experiments. Analytical Modeling provides rapid sizing, FEA combined with a GA refines geometry, and targeted experiments quantify nonlinear effects (friction, wear, thermal variability, and dynamic resonances). Unlike conventional methods, the integration is performed within iterative loops, using empirical data to refine simulation assumptions. As a result, development time is reduced by 30% and nonlinear effects are precisely addressed. The method is demonstrated on an automotive-grade EMLA. Its design is based on a claw-pole Permanent Magnet Stepper Motor, a trapezoidal lead screw, and an open-loop control with Hall effect end-position detection. After applying the method, the EMLA delivers more than 40 N of push force and achieves 600,000 actuations under the required conditions, making it suitable for various applications. Full article
(This article belongs to the Section High Torque/Power Density Actuators)
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23 pages, 3201 KB  
Review
Control Algorithms in Robot-Assisted Rehabilitation: A Systematic Review
by Ovidiu Liviu Rad and Cornel Brisan
Appl. Sci. 2025, 15(16), 9184; https://doi.org/10.3390/app15169184 - 21 Aug 2025
Viewed by 1515
Abstract
Robotic-assisted rehabilitation has become an essential field in supporting the functional recovery of patients with neurological, musculoskeletal or post-traumatic conditions. This paper provides a systematic and applicative analysis of the control algorithms used in robotic rehabilitation systems, with a focus on the functional [...] Read more.
Robotic-assisted rehabilitation has become an essential field in supporting the functional recovery of patients with neurological, musculoskeletal or post-traumatic conditions. This paper provides a systematic and applicative analysis of the control algorithms used in robotic rehabilitation systems, with a focus on the functional classification: position control, force, impedance, adaptive, artificial intelligence-based and hybrid schemes. The characteristics of each type of control, clinical applications, advantages and technical limitations are discussed in detail, illustrated by block diagrams and comparative graphs. The paper also includes a synthesis of existing commercial systems, a multi-criteria evaluation of the performance of the algorithms and an analysis of emerging trends in the recent literature (2020–2024). Current challenges regarding sensor integration, system standardization, real-time clinical feasibility and the applicability of brain–machine interfaces or adaptive myoelectric prostheses are discussed. The results obtained can support the development of efficient, safe and personalized solutions in the field of robotic rehabilitation. Full article
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19 pages, 6153 KB  
Article
Copper–PLLA-Based Biopolymer Wrinkle Structures for Enhanced Antibacterial Activity
by Petr Slepička, Iva Labíková, Bára Frýdlová, Aneta Pagáčová, Nikola Slepičková Kasálková, Petr Sajdl and Václav Švorčík
Polymers 2025, 17(16), 2173; https://doi.org/10.3390/polym17162173 - 8 Aug 2025
Viewed by 623
Abstract
The increasing prevalence of antibiotic-resistant bacteria has intensified the need for innovative antibacterial surfaces, particularly in biomedical applications. Traditional approaches often rely on chemical agents alone, which may lead to diminishing efficacy over time. To address this, we investigated the development of a [...] Read more.
The increasing prevalence of antibiotic-resistant bacteria has intensified the need for innovative antibacterial surfaces, particularly in biomedical applications. Traditional approaches often rely on chemical agents alone, which may lead to diminishing efficacy over time. To address this, we investigated the development of a novel antibacterial surface by combining the inherent antimicrobial properties of copper with an engineered surface topography on a biopolymer matrix. A copper–poly-L-lactic acid (Cu-PLLA) composite system was fabricated using sputtering deposition followed by controlled thermal treatment to induce wrinkle-like micro- and nanostructures on the surface. The surface morphology was characterized using scanning electron microscopy (SEM) and atomic force microscopy (AFM), confirming the formation of hierarchical wrinkle patterns. The chemical composition and distribution of copper were analyzed via energy-dispersive X-ray spectroscopy (EDS). Antibacterial performance was assessed against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus using standard colony count reduction assays. The Cu-PLLA wrinkled surfaces demonstrated significantly enhanced bactericidal activity compared with flat PLLA and copper-free controls, a finding attributed to a synergistic effect of mechanical membrane disruption and copper-mediated chemical toxicity. These findings suggest that biopolymer–metal hybrid surfaces with engineered topography offer a promising strategy for developing next-generation antibacterial materials suitable for biomedical and clinical use. Full article
(This article belongs to the Special Issue Feature Papers in Polymer Science and Technology)
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22 pages, 397 KB  
Review
Compliant Force Control for Robots: A Survey
by Minglei Zhu, Dawei Gong, Yuyang Zhao, Jiaoyuan Chen, Jun Qi and Shijie Song
Mathematics 2025, 13(13), 2204; https://doi.org/10.3390/math13132204 - 6 Jul 2025
Cited by 2 | Viewed by 3302
Abstract
Compliant force control is a fundamental capability for enabling robots to interact safely and effectively with dynamic and uncertain environments. This paper presents a comprehensive survey of compliant force control strategies, intending to enhance safety, adaptability, and precision in applications such as physical [...] Read more.
Compliant force control is a fundamental capability for enabling robots to interact safely and effectively with dynamic and uncertain environments. This paper presents a comprehensive survey of compliant force control strategies, intending to enhance safety, adaptability, and precision in applications such as physical human–robot interaction, robotic manipulation, and collaborative tasks. The review begins with a classification of compliant control methods into passive and active approaches, followed by a detailed examination of direct force control techniques—including hybrid and parallel force/position control—and indirect methods such as impedance and admittance control. Special emphasis is placed on advanced compliant control strategies applied to structurally complex robotic systems, including aerial, mobile, cable-driven, and bionic robots. In addition, intelligent compliant control approaches are systematically analyzed, encompassing neural networks, fuzzy logic, sliding mode control, and reinforcement learning. Sensorless compliance techniques are also discussed, along with emerging trends in hardware design and intelligent control methodologies. This survey provides a holistic view of the current landscape, identifies key technical challenges, and outlines future research directions for achieving more robust, intelligent, and adaptive compliant force control in robotic systems. Full article
(This article belongs to the Special Issue Intelligent Control and Applications of Nonlinear Dynamic System)
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15 pages, 4545 KB  
Article
Research on Environmental Adaptability of Force–Position Hybrid Control for Quadruped Robots Based on Model Predictive Control
by Yuquan Xue, Liming Wang, Bi He, Yonghui Zhao, Yang Wang and Longmei Li
Electronics 2025, 14(8), 1604; https://doi.org/10.3390/electronics14081604 - 16 Apr 2025
Cited by 1 | Viewed by 959
Abstract
This study proposes a force–position hybrid control method for quadruped robots based on the Model Predictive Control (MPC) algorithm, aiming to address the challenges of stability and adaptability in complex terrain environments. Traditional control methods for quadruped robots are often based on simplified [...] Read more.
This study proposes a force–position hybrid control method for quadruped robots based on the Model Predictive Control (MPC) algorithm, aiming to address the challenges of stability and adaptability in complex terrain environments. Traditional control methods for quadruped robots are often based on simplified models, neglecting the impact of complex terrains and unstructured environments on control performance. To enhance the real-world performance of quadruped robots, this paper employs the MPC algorithm to integrate force and position control to achieve precise force–position hybrid regulation. By transforming foot-end forces into joint torques and optimizing them using kinematic inverse solutions, the robot’s stability and motion accuracy in challenging terrains is further enhanced. In this study, a Kalman filter-based state estimation method is adopted to estimate the robot’s state in real time, enabling closed-loop control through the MPC framework, combined with kinematic inverse solutions for hybrid control. The experimental results demonstrate that the proposed MPC algorithm significantly improves the robot’s adaptability and control accuracy across various terrains. In particular, it exhibits superior performance and robustness in multi-contact and uneven terrain scenarios. This research provides a novel approach for deploying quadruped robots in dynamic and complex environments and offers strong support for further optimization of motion control strategies. Full article
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29 pages, 20381 KB  
Article
A Study on the Force/Position Hybrid Control Strategy for Eight-Axis Robotic Friction Stir Welding
by Wenjun Yan and Yue Yu
Metals 2025, 15(4), 442; https://doi.org/10.3390/met15040442 - 16 Apr 2025
Viewed by 1315
Abstract
In aerospace and new-energy vehicle manufacturing, there is an increasing demand for the high-quality joining of large, curved aluminum alloy structures. This study presents a robotic friction stir welding (RFSW) system employing a force/position hybrid control. An eight-axis linkage platform integrates an electric [...] Read more.
In aerospace and new-energy vehicle manufacturing, there is an increasing demand for the high-quality joining of large, curved aluminum alloy structures. This study presents a robotic friction stir welding (RFSW) system employing a force/position hybrid control. An eight-axis linkage platform integrates an electric spindle, multidimensional force sensors, and a laser displacement sensor, ensuring trajectory coordination between the robot and the positioner. By combining long-range constant displacement with small-range constant pressure—supplemented by an adaptive transition algorithm—the system regulates the axial stirring depth and downward force. The experimental results confirm that this approach effectively compensates for robotic flexibility, keeping weld depth and pressure deviations within 5%, significantly improving seam quality. Further welding verification was performed on typical curved panels for aerospace applications, and the results demonstrated strong adaptability under high-load, multi-DOF conditions, without crack formation. This research could advance the field toward more robust, automated, and adaptive RFSW solutions for aerospace, automotive, and other high-end manufacturing applications. Full article
(This article belongs to the Section Welding and Joining)
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18 pages, 5498 KB  
Article
Development and Evaluation of a Novel Upper-Limb Rehabilitation Device Integrating Piano Playing for Enhanced Motor Recovery
by Xin Zhao, Ying Zhang, Yi Zhang, Peng Zhang, Jinxu Yu and Shuai Yuan
Biomimetics 2025, 10(4), 200; https://doi.org/10.3390/biomimetics10040200 - 25 Mar 2025
Cited by 1 | Viewed by 984
Abstract
This study developed and evaluated a novel upper-limb rehabilitation device that integrates piano playing into task-oriented occupational therapy, addressing the limitations of traditional continuous passive motion (CPM) training in patient engagement and functional recovery. The system features a bi-axial sliding platform for precise [...] Read more.
This study developed and evaluated a novel upper-limb rehabilitation device that integrates piano playing into task-oriented occupational therapy, addressing the limitations of traditional continuous passive motion (CPM) training in patient engagement and functional recovery. The system features a bi-axial sliding platform for precise 61-key positioning and a ten-link, four-loop robotic hand for key striking. A hierarchical control framework incorporates MIDI-based task mapping, finger optimization using an improved Hungarian algorithm, and impedance–admittance hybrid control for adaptive force–position modulation. An 8-week randomized controlled trial demonstrated that the experimental group significantly outperformed the control group, with a 74.7% increase in Fugl–Meyer scores (50.5 ± 2.5), a 14.6-point improvement in the box and block test (BBT), a 20.2-s reduction in nine-hole peg test (NHPT) time, and a 72.6% increase in rehabilitation motivation scale (RMS) scores (55.4 ± 3.8). The results indicate that combining piano playing with robotic rehabilitation enhances neuroplasticity and engagement, significantly improving motor function, daily activity performance, and rehabilitation adherence. This mechanical-control synergy introduces a new paradigm for music-interactive rehabilitation, with potential applications in home-based remote therapy and multimodal treatment integration. Full article
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16 pages, 7495 KB  
Article
Optimization of Structural Parameters and Mechanical Performance Analysis of a Novel Redundant Actuation Rehabilitation Training Robot
by Junyu Wu, He Wang, Yubin Liu, Zhuoqi Man, Xiaofan Yang, Xuanming Cao, Hegao Cai and Jie Zhao
Biomimetics 2025, 10(4), 199; https://doi.org/10.3390/biomimetics10040199 - 25 Mar 2025
Cited by 1 | Viewed by 588
Abstract
The integration of redundant structures into robotic systems enhances the degrees of freedom (DOFs), flexibility, and capability to perform complex tasks. This study evaluates the mechanical performance of a 9-DOF series-parallel hybrid redundant device designed for rehabilitation training of patients with balance disorders. [...] Read more.
The integration of redundant structures into robotic systems enhances the degrees of freedom (DOFs), flexibility, and capability to perform complex tasks. This study evaluates the mechanical performance of a 9-DOF series-parallel hybrid redundant device designed for rehabilitation training of patients with balance disorders. The redundant structural design improves the robot’s movement flexibility, optimizes load distribution, and mitigates stress concentration in local joints or components. To optimize the robot’s overall structural parameters and reduce joint driving forces, a genetic algorithm (GA) was employed. A custom dataset was created by collecting motion-related data, including foot posture and position. The robot’s mechanical characteristics were comprehensively analyzed, followed by simulation experiments. The results demonstrate that incorporating the redundant structure, along with the optimization of structural parameters, significantly enhances the robot’s mechanical performance. This study provides a solid foundation for the functional development and control system design of rehabilitation robots, extending the capabilities of existing systems and offering a novel, reliable, and efficient therapeutic tool for patients with balance disorders. Full article
(This article belongs to the Special Issue Recent Advances in Robotics and Biomimetics)
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16 pages, 2270 KB  
Article
Lodging Resistance of Japonica Hybrid Rice Plants Studied in Relation to Mechanical and Physicochemical Characteristics
by Liying Zhang, Zuobin Ma, Na He, Zhiqiang Tang, Changhua Wang, Wenjing Zheng, Hui Wang, Guomin Sui, Hong Gao and Lili Wang
Agronomy 2025, 15(3), 699; https://doi.org/10.3390/agronomy15030699 - 13 Mar 2025
Cited by 4 | Viewed by 1120
Abstract
The research on rice lodging resistance holds immeasurable value for achieving high yield, stable production, and superior quality of rice. To investigate the effects of mechanical properties and physicochemical characteristics of Japonica hybrid rice on its lodging resistance ability under natural field cultivation [...] Read more.
The research on rice lodging resistance holds immeasurable value for achieving high yield, stable production, and superior quality of rice. To investigate the effects of mechanical properties and physicochemical characteristics of Japonica hybrid rice on its lodging resistance ability under natural field cultivation conditions, LY1052, LY9906, and GY1, which were mainly popularized in northern China, were selected as the experimental subjects, and NL313, Japonica hybrid rice prone to lodging, was taken as the control (NL313).The max bending force, breaking moment, bending section coefficient, single stem weight mass moment, bending strength, Young’s elastic modulus, inertia moment, and other mechanical indexes were measured by the bending test and tensile test, and the correlations between mechanical indexes, physicochemical indexes, and lodging index were studied. There was an extremely significant difference in the lodging index of experimental subjects and control (NL313) (p < 0.05). Therefore, it was concluded that the lower plant height and lighter panicle were not the stronger lodging resistance under appropriate cultivation conditions. Optimization of rice plant-type structure can achieve the unity of high culm and high yield. The lodging resistance of rice could be improved by shortening the internode length, increasing the tissue thickness and vascular bundle area, and increasing the content of cellulose and potassium in the stem. It was also found that the lodging resistance of rice plants was positively correlated with the maximum stem bending force, breaking moment, bending section coefficient, bending strength, and Young’s elastic modulus (p < 0.01) and negatively correlated with single stem weight mass moment and inertia moment (p < 0.01). It is feasible to select them as reference indexes of the lodging resistance of rice. The experimental results not only help to enrich the theoretical system of rice lodging resistance research but also provide an essential reference and basis for formulating scientific cultivation and management measures and breeding lodging-resistant rice varieties in practical production, which is of great significance for ensuring global food security and promoting sustainable agricultural development. Full article
(This article belongs to the Special Issue Crop Production in the Era of Climate Change)
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24 pages, 16293 KB  
Article
Multi-Control Strategies on a Cubic–Quintic Nonlinear Hybrid Oscillator with External Excitation Under Resonance Conditions
by Asma Alanazy, Yasser A. Amer, Ashraf Taha EL-Sayed, Fatma Sh. Mohammed and Taher A. Bahnasy
Mathematics 2025, 13(6), 957; https://doi.org/10.3390/math13060957 - 13 Mar 2025
Viewed by 2416
Abstract
The goal of this article is to reduce the vibration of a hybrid oscillator with a cubic–quintic nonlinear term under internal and external forces in the worst resonance case. To eliminate the harmful vibration in the system, the following strategies are suggested: nonlinear [...] Read more.
The goal of this article is to reduce the vibration of a hybrid oscillator with a cubic–quintic nonlinear term under internal and external forces in the worst resonance case. To eliminate the harmful vibration in the system, the following strategies are suggested: nonlinear derivative feedback control (NDF), linear negative velocity feedback control (LNVC), nonlinear integral positive position feedback (NIPPF), integral resonant control (IRC), negative velocity with time delay (TD), and positive position feedback (PPF). It is discovered that the PPF control suppresses vibration more effectively than typical controllers, which reduces the vibration to 0.0001 with an effectiveness of 99.92%. Moreover, the main advantages of the PPF controller are its low cost and the fast response. The multiple time scale perturbation technique (MSPT) is used to apply the theoretical methodology and obtain a perturbed response. In order to close the concurrent primary and internal resonance case, frequency response (FR) equations are also used to study and analyze the system’s stability. The MATLAB software is used to complete and clarify all numerical topics. The FR curves are examined to determine the amplitude’s subsequent impact from variations in the parameters’ values. Lastly, a comparison of the numerical and analytical solutions is performed utilizing time history. Along with comparing the impact of our PPF damper on the hybrid oscillator, earlier research is also provided. Full article
(This article belongs to the Section C2: Dynamical Systems)
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20 pages, 9029 KB  
Article
Enhancing Continuum Robotics Accuracy Using a Particle Swarm Optimization Algorithm and Closed-Loop Wire Transmission Model for Minimally Invasive Thyroid Surgery
by Na Guo, Haoyun Zhang, Xingshuai Li, Xinnan Cui, Yang Liu, Jiachen Pan, Yajuan Song and Qinjian Zhang
Appl. Sci. 2025, 15(4), 2170; https://doi.org/10.3390/app15042170 - 18 Feb 2025
Cited by 2 | Viewed by 934
Abstract
To address the challenges of confined workspaces and high-precision requirements in thyroid surgery, this paper proposes a modular cable-driven robotic system with a hybrid rigid–continuum structure. By integrating rigid mechanisms and continuum joints within a closed-loop cable-driven framework, the system achieves a balance [...] Read more.
To address the challenges of confined workspaces and high-precision requirements in thyroid surgery, this paper proposes a modular cable-driven robotic system with a hybrid rigid–continuum structure. By integrating rigid mechanisms and continuum joints within a closed-loop cable-driven framework, the system achieves a balance between flexibility in narrow spaces and operational stiffness. To tackle kinematic model inaccuracies caused by manufacturing errors, an innovative joint decoupling strategy combined with the Particle Swarm Optimization (PSO) algorithm is developed to dynamically identify and correct 19 critical parameters. Experimental results demonstrate a 37.74% average improvement in repetitive positioning accuracy and a 52% reduction in maximum absolute error. However, residual positioning errors (up to 4.53 mm) at motion boundaries highlight the need for integrating nonlinear friction compensation. The feasibility of a safety-zone-based force feedback master–slave control strategy is validated through Gazebo simulations, and a ring-grasping experiment on a surgical training platform confirms its clinical applicability. Full article
(This article belongs to the Special Issue Control and Application for Biorobotics)
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23 pages, 11170 KB  
Article
Automatic Robotic Ultrasound for 3D Musculoskeletal Reconstruction: A Comprehensive Framework
by Dezhi Sun, Alessandro Cappellari, Bangyu Lan, Momen Abayazid, Stefano Stramigioli and Kenan Niu
Technologies 2025, 13(2), 70; https://doi.org/10.3390/technologies13020070 - 8 Feb 2025
Cited by 4 | Viewed by 3571
Abstract
Musculoskeletal ultrasound (US) imaging faces challenges such as operator experience, limited spatial flexibility, and high personnel costs. This study introduces an Automated Robotic Ultrasound Scanning (ARUS) system that integrates key technological advancements to automate the ultrasound scanning procedure with the robot, including anatomical [...] Read more.
Musculoskeletal ultrasound (US) imaging faces challenges such as operator experience, limited spatial flexibility, and high personnel costs. This study introduces an Automated Robotic Ultrasound Scanning (ARUS) system that integrates key technological advancements to automate the ultrasound scanning procedure with the robot, including anatomical target localization, automatic trajectory generation, deep-learning-based segmentation, and 3D reconstruction of musculoskeletal structures. The ARUS system consists of a robotic arm, ultrasound imaging, and stereo vision for precise anatomical area detection. A Graphical User Interface (GUI) facilitates a flexible selection of scanning trajectories, improving user interaction and enabling customized US scans. To handle complex and dynamic curvatures on the skin, together with anatomical area detection, the system employs a hybrid position–force control strategy based on the generated trajectory, ensuring stability and accuracy. Additionally, the utilized RA-UNet model offers multi-label segmentation on the bone and muscle tissues simultaneously, which incorporates residual blocks and attention mechanisms to enhance segmentation accuracy and robustness. A custom musculoskeletal phantom was used for validation. Compared to the reference 3D reconstruction result derived from the MRI scan, ARUS achieved a 3D reconstruction root mean square error (RMSE) of 1.22 mm, with a mean error of 0.94 mm and a standard deviation of 0.77 mm. The ARUS system extends 3D musculoskeletal imaging capacity by enabling both bones and muscles to be segmented and reconstructed into 3D shapes in real time and simultaneously. These features suggest significant potential as a cost-effective and reliable option for musculoskeletal examination and diagnosis in real-time applications. Full article
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16 pages, 2554 KB  
Article
Development, Characterization, and Evaluation of Chi-Tn mAb-Functionalized DOTAP-PLGA Hybrid Nanoparticles Loaded with Docetaxel for Lung Cancer Therapy
by Analía Castro, Álvaro Pittini, Nora Berois, Ricardo Faccio, Pablo Miranda, Álvaro W. Mombrú, Eduardo Osinaga and Helena Pardo
Pharmaceutics 2025, 17(2), 164; https://doi.org/10.3390/pharmaceutics17020164 - 25 Jan 2025
Cited by 1 | Viewed by 1709
Abstract
Background/Objectives: The focus of this study was to prepare and characterize docetaxel (DCX)-loaded lipid/polymer hybrid nanoparticles (LPHNps) functionalized with the monoclonal antibody (mAb) Chi-Tn for a potential active targeting approach in lung cancer treatment. Methods: We synthesized DOTAP-PLGA hybrid nanoparticles loaded [...] Read more.
Background/Objectives: The focus of this study was to prepare and characterize docetaxel (DCX)-loaded lipid/polymer hybrid nanoparticles (LPHNps) functionalized with the monoclonal antibody (mAb) Chi-Tn for a potential active targeting approach in lung cancer treatment. Methods: We synthesized DOTAP-PLGA hybrid nanoparticles loaded with DCX and functionalized them with Chi-Tn mAb through a biotin–avidin approach. The physicochemical characterization involved dynamic light scattering, transmission electron microscopy, Raman spectroscopy, and atomic force microscopy. The in vitro and in vivo evaluations encompassed uptake studies, cell viability tests, and the assessment of tumor growth control in a lung cancer model. Results: The nanoparticles featured a hydrophobic PLGA core with 99.9% DCX encapsulation efficiency, surrounded by a DOTAP lipid shell ensuring colloidal stability with a high positive surface charge. The incorporation of PEGylated lipids on their surface helps evade the immune system and facilitate Chi-Tn mAb attachment. The resulting nanoparticles exhibit a spherical shape with monodisperse particle sizes averaging 250 nm, and demonstrate sustained drug release. In vitro uptake studies and viability assays conducted in A549 cancer cells show that the Chi-Tn mAb enhances nanoparticle internalization and significantly reduces cell viability. In vivo studies demonstrate a notable reduction in tumor volume and an increased survival rate in the A549 tumor xenograft mice model when DCX was encapsulated in nanoparticles and targeted with Chi-Tn mAb in comparison to the free drug. Conclusions: Therefore, Chi-Tn-functionalized LPHNps hold promise as carriers for actively targeting DCX to Tn-expressing carcinomas. Full article
(This article belongs to the Section Nanomedicine and Nanotechnology)
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