Search Results (1,855)

As the development of the ocean extends to the deep and open seas, the application of multi-hull floating systems is becoming increasingly widespread, covering offshore oil and gas transfer and material replenishment operations. In multi-body floating systems, the hydrodynamic interactions between adjacent floating bodies significantly affect the overall motion response and load distribution. However, there is currently a lack of systematic experimental research on systems involving three or more units under the combined action of wind, waves, and currents. This study presents a 1:50 scale model experiment on a five-body offshore replenishment station, comprising a central transfer platform and four surrounding vessels. Absolute six-degree-of-freedom motions and relative displacements between the transfer platform and neighboring vessels were measured. The results indicate distinct differences among the units. The peripheral vessels have greater horizontal and yaw motions, while the central units are more restricted. The relative motions are substantially increased for beam and oblique wave conditions, implying increased interaction effects in the gaps between neighboring bodies. Moreover, the combined oblique environmental loading and asymmetric mooring stiffness result in increased global drift and yaw motions. These findings provide benchmark data for numerical validation and practical guidance for the design and operation of multi-body floating systems. Full article

Background/Objectives: Antimicrobial agents have revolutionized disease management in humans and animals; however, their misuse and overuse have accelerated the emergence and spread of antimicrobial resistance (AMR) and antimicrobial resistance genes (ARGs). Dairy farms are recognized as potential hotspots for ARG dissemination, particularly through Escherichia coli, which acts as a reservoir and vector of ARGs, enabling their horizontal transfer via plasmids and other mobile genetic elements. This study aimed to characterize the genomic diversity, ARG profiles, plasmid content, and phylogenetic relationships of E. coli isolated from dairy farm environments and milk using whole-genome sequencing. Methods: A total of 31 E. coli isolates recovered from soil, effluent, cow dung, and milk samples underwent deoxyribonucleic acid extraction, library preparation, and sequencing on the Illumina MiSeq platform, followed by comprehensive bioinformatic analysis. Results: The E. coli isolates exhibited 20 distinct sequence types, including one novel sequence type. Plasmids were detected in 71% of the isolates, with the IncF plasmid family being the most predominant. Furthermore, 12 ARG groups were identified, with β-lactam resistance genes detected in 67.7% of isolates. Notably, blaCTX-M genes were identified in all phenotypically confirmed extended-spectrum β-lactamase-producing isolates. Additional ARGs, including those conferring resistance to tetracyclines (tet(A), tetX4), quinolones (qnrS1), aminoglycosides (aph, aad, ant), and folate pathway inhibitors (dfr and sul), were widely distributed throughout the samples. Phylogenetic analysis revealed clustering of isolates from different sample types, particularly among ST58 isolates, suggesting cross-environmental transmission. Conclusions: This study demonstrates that E. coli from dairy farm environments harbor diverse ARGs and plasmids, confirming their role as reservoirs of AMR. These findings underscore the importance of prudent antimicrobial use, routine genomic surveillance, and enhanced biosecurity measures to limit cross-environmental transmission. Full article

This study examines the influence of photobioreactor (PBR) configuration on the cultivation performance of Chlorococcum sp. using aquaculture wastewater as the growth medium. Four systems were compared: horizontal without aeration (H-Plain), horizontal with aeration (H-Aerated), vertical with aeration (V-Aerated), and vertical with aeration and red LED illumination (V-LED). Over 14 days, the V-LED system achieved the highest biomass concentration (0.50 g L⁻¹) and volumetric productivity (0.063 g L⁻¹ day⁻¹), accompanied by nitrate and phosphate removals of 94% and 55.6%, respectively. Statistical analysis (ANOVA, p < 0.05) confirmed significant differences among configurations, demonstrating that light quality and aeration act synergistically to enhance growth and nutrient assimilation. While aeration improved CO₂ transfer and mixing, it was insufficient without adequate photon delivery. Conversely, red LED illumination mitigated photolimitation in vertical systems, promoting efficient photosynthesis and nutrient uptake. Energy assessment revealed that V-LED offered the highest productivity in expense of power input (1.08 kWh day⁻¹). These findings highlight the critical role of integrated PBR design, emphasizing that optimal combinations of geometry, aeration, and spectral lighting as keys to achieving high biomass yields and efficient nutrient removal in sustainable microalgae-based wastewater treatment systems. Full article

The present study examines the regulatory effects of laser heating parameters (power, position, and spot radius) on hydrothermal wave instability, heat and mass transfer, and interfacial deformation in bilayer thermocapillary systems under normal gravity. It provides theoretical support for the efficient utilization of energy and the optimization of industrial thermal systems, meeting the demands of sustainable development. The results show that increasing laser power induces asymmetric flow bifurcation nears the laser heating point, enhancing hydrothermal waves in the left region while suppressing them in the right region, with oscillation periods decreasing monotonically and amplitudes showing non-monotonic variation. Laser heating position alters convection intensity distribution, in which the convection in the hot zone is weakened as the laser point nears the cold end, while the convection in the cold zone is strengthened as the laser point nears the hot end. Reducing spot radius significantly decreases temperature gradients near the interfacial heat source, while attenuating horizontal velocity amplitude and increasing oscillation period, effectively suppressing oscillatory thermocapillary convection. This study demonstrates that precise control of laser heating parameters can effectively suppress thermocapillary instability and optimize heat transfer without introducing additional mechanical disturbances. It provides a theoretical basis for efficient, low-energy, non-contact thermal flow control technologies. Full article

Motor vehicle encroachment into non-motorized lanes is a common but hard-to-verify violation in urban intersections, especially when monitored from unmanned aerial vehicles (UAVs) or high-mounted overhead views. Existing rule-based solutions built on horizontal bounding boxes and center-point/line-crossing criteria are sensitive to perspective distortion, occlusion, and frame-to-frame jitter, resulting in unstable decisions and low evidential value. This paper presents a cascaded UAV-view system that closes the loop from perception to evidence output through detection–segmentation–recognition–decision. First, we adopt a two-stage detection cascade: a lightweight vehicle detector localizes vehicles using axis-aligned bounding boxes, and a dedicated YOLOv5n-based oriented bounding box (OBB) license plate detector, constructed via architecture grafting and weight transfer, is then applied within each vehicle region of interest (ROI) to localize rotated license plates under large pose variation and small-target conditions. Second, a U-Net lane region segmentation module provides pixel-level spatial constraints to define an enforceable lane occupancy region. Third, a perspective rectification step is integrated with the PP-OCRv4 optical character recognition (OCR) framework to improve license plate recognition reliability for tilted plates. Finally, an area ratio criterion and an N-frame temporal counter are used to suppress transient misdetections and stabilize alarms. On a representative 100-sample controlled encroachment benchmark, the proposed system improves detection accuracy from 67.0% to 92.0% and reduces the false positive rate from 32.35% to 5.88% compared with a baseline horizontal bounding box (HBB)-based rule. The system outputs both violation alarms and license plate evidence, supporting practical deployment for multi-view traffic governance. Full article

To address the issue of significantly reduced coupling coefficient and limited transmission efficiency in traditional flat solenoid magnetic couplers within wireless power transfer (WPT) systems under horizontal lateral offset conditions, this paper proposes a design method for a concave flat solenoid coil magnetic coupler for engineering applications, aiming to achieve high misalignment tolerance. An equivalent model of the LCC/S compensation circuit is established, its output characteristics are analyzed, and the parameter configuration method for its resonant elements is derived. Secondly, from the perspective of winding arrangement, the mechanism by which the coil winding method, turn spacing, and port concavity angle affect the uniformity of magnetic field distribution and the retention rate of the coupling coefficient is analyzed in detail, and corresponding parameter trade-off and optimization methods are proposed. Subsequently, a simulation model of multiple configuration magnetic couplers is established based on Ansys/Maxwell, comparing the magnetic field distribution and coupling coefficient variation of different structures under horizontal offset conditions. The results show that the concave structure with a non-uniform arrangement and a port concavity angle of 30° can still maintain a high coupling coefficient and stable transmission performance under a maximum horizontal offset equal to 60% of the corresponding transmitter-side characteristic dimension. To achieve lightweight and integrated design, the receiver is designed with a flexible printed circuit board (FPC) coil structure, meeting the miniaturization and high power density requirements of low-to-medium power portable devices. Finally, a 100 W experimental prototype was built. Experimental results show that within an offset range of ±15 mm on the X-axis and ±30 mm on the Y-axis at the receiver, the system output voltage fluctuation is controlled within 4%, and the maximum transmission efficiency reaches 87.3%. These results verify the feasibility and practical applicability of the proposed magnetic coupler with high misalignment tolerance. Full article

Adaptive façade systems are increasingly used to mitigate glare in daylit spaces and improve visual comfort while supporting sustainable daylight utilisation and reduced reliance on electric lighting in buildings. However, their performance is often evaluated using illuminance-based metrics or uncalibrated simulations, limiting the reliability of glare assessment. This study proposes a calibrated experimental–simulation framework for evaluating glare reduction achieved by a kinetic horizontal shading system (KSS) under real daylight conditions. The approach integrates reduced-scale physical measurements with Radiance-based simulations using a digitally reconstructed twin of the experimental setup. Two geometrically identical test chambers positioned side-by-side—a static reference chamber and a kinetic chamber equipped with six adaptive fins (0.63 m real-scale depth)—were investigated using a 1:20 scale mock-up. Internal illuminance measurements were normalised between chambers, and a sky-scaling procedure was applied to calibrate simulated sky luminance distributions against measured data on an hourly basis, enabling photometrically validated HDR renderings for glare evaluation. Glare performance was analysed for three representative clear-sky days during periods of maximum solar exposure (11:00–17:00) under late-summer conditions at approximately 51° N latitude in Wrocław, Poland. Visual comfort was assessed using Daylight Glare Probability (DGP), Daylight Glare Index (DGI), and veiling luminance (L_veil). The kinetic shading system reduced mean DGP from 0.57 to 0.35 (−38%) and peak glare values by nearly half compared with the static configuration, while veiling luminance decreased by 73%, indicating substantial improvement in physiological visual comfort. These results demonstrate that adaptive fin movement effectively suppresses both perceptual and physiological glare during critical daylight hours. The proposed calibrated experimental–simulation workflow provides a robust and transferable methodology for evaluating the glare performance of adaptive façade systems and supports sustainable daylight management by enabling high daylight availability while maintaining acceptable glare levels in buildings. Full article

Several studies have investigated counterflow and concurrent flow in channels separated by a membrane to simulate mass transfer through membranes; however, few of them have used computational fluid dynamics (CFD). The current study aimed to numerically simulate and physically describe the distribution of pressure and velocity in counter-current flow by solving Navier-Stokes (N-S) equations in the channel and membrane pores (vertical channels). This is in contrast to most previous studies, in which the channel flow was simulated using N-S equations while ultra-filtration membrane flow was simulated using Darcy’s law. Consequently, the current study was executed using a CFD simulation to achieve several significant features: avoiding the execution of experimental tests, reducing the effort of model design and the expense and time consumption of fabrication, and facilitating the easy observation of variations in the pressure and the horizontal and vertical velocity for each point in the model. Two-dimensional CFD methods directly simulated the flow in channels and membrane pores to solve the N-S equations for each point in the whole domain, for which the velocity (horizontal and vertical) and pressure were calculated. In the current study, it was found that the pressure decreased from the inlet to the outlet of the channel, the horizontal velocity decreased from the inlet to the middle of the channel length and then increased to the outlet of the channel, and the vertical velocity decreased from the inlet to the middle of the channel length (L/2) with an upward direction (positive) and from L/2 to the outlet of the channel with a downward direction (negative). The analytical solution (1D model) was used to validate a numerical simulation (CFD) for the current study, but there were slight differences in the results between them. The results were perfectly explored and displayed the flow distribution patterns inside the channels and the membrane pores (vertical channels). The current study model represents the hemodialysis process. Full article

Brucella anthropi is an emerging opportunistic pathogen characterized by intrinsic resistance to most $\beta$-lactams. However, the acquisition of carbapenem resistance in this species has rarely been documented in environmental, animal, or clinical settings. In this study, a multidrug-resistant strain, SBA01, was isolated from wastewater-irrigated soil. SBA01 exhibited phenotypic resistance to carbapenems and colistin, the latter being independent of mcr genes. Genomic analysis localized bla_IMP-1 on a stable 21 kb plasmid maintained by a Type II toxin–antitoxin system. While non-self-transmissible, this plasmid was mobilized to Escherichia coli and Klebsiella pneumoniae via an unclassified 50 kb helper plasmid. Additionally, a 217 kb prophage-bearing megaplasmid was identified, enhancing genomic plasticity. Genomic screening identified 32 putative virulence determinants, including markers associated with host interaction. Risk profiling indicated an elevated hazard index for SBA01, driven by the convergence of multidrug resistance, cryptic mobilization capacity, and opportunistic survival traits. These findings position B. anthropi as a resilient environmental reservoir for clinically relevant carbapenemases. Expanding surveillance frameworks to include such adaptive hosts is necessary to better evaluate potential occupational exposures at the wastewater–soil interface. Full article

Background: Wastewater treatment plants (WWTPs) are hotspots of antimicrobial resistance (AMR) due to inputs from diverse anthropogenic sources. Aeromonas spp., ubiquitous in aquatic environments, often carry clinically relevant antibiotic resistance genes (ARGs) and can persist beyond fecal contamination indicators, making them promising sentinel organisms for AMR dissemination. The aim of this study was to assess the suitability of Aeromonas spp. in this role by characterizing resistance profiles, associated virulence factor genes (VFGs), genetic mobility, and persistence across wastewater and drinking water treatment processes in the Barcelona metropolitan area, Spain. Methods: Isolates were phenotypically characterized and screened for ARGs, VFGs, integrons, and heavy metal tolerance genes, followed by whole-genome sequencing (WGS). Biofilm formation was assessed in vitro. Conjugation assays with Escherichia coli evaluated horizontal gene transfer (HGT) potential. Results: A total of 428 antibiotic-resistant Aeromonas spp., the most abundant antibiotic-resistant bacteria isolated during the 2023 sampling campaigns from two WWTPs and one drinking water treatment plant (DWTP), were characterized. Trimethoprim/sulfamethoxazole (SXT) non-susceptibility was most frequent (72%), followed by cefoxitin resistance (65.4%). The sul1 (57.5%) and bla_MOX (78.6%) genes predominated among SXT- and β-lactam-resistant isolates. The merA gene was detected in 23.6%; 97.9% harbored at least one VFG (aerA, act, fla, alt, or hlyA), and 70.3% carried intI1. Half formed biofilm. Conjugation confirmed bi-directional HGT, and WGS revealed persistent ST3458 clones across treatment stages. Conclusions: WWTPs and DWTPs act as reservoirs of antibiotic-resistant Aeromonas spp., demonstrating persistence and HGT potential. Findings support their use as sentinel organisms for AMR surveillance in aquatic environments and for assessing treatment efficacy, highlighting variability across treatment types and locations, and reinforcing their relevance for urban water reclamation monitoring. Full article

Dual-phase layered microstructures containing alternating regions of soft and hard phases can produce alloys with a unique combination of strength and ductility. In this study, the molecular dynamics (MD) method was utilized to simulate nanoindentation of a Ni/NiTi/Ni nanostructured film (NSF). This film features a unique alternating FCC/B2/FCC microstructure, in which the B2-phase NiTi acts as a superelastic shape memory alloy (SMA). The results indicate that Ni/NiTi/Ni NSF significantly reduces its hardness due to the superelasticity of the B2 phase. The presence of the NiTi interlayer effectively blocks the propagation path of dislocations and stacking faults by transforming the local dislocations transferred from the upper layer into a large-scale coordinated phase transition, significantly reducing local deformation misalignment. As the thickness of the surface film λ increases, the dislocation slip plane propagating horizontally appears in the upper pure Ni layer. The thicker the surface film, the more horizontal slip planes are formed. This study provides new insights into the contact mechanical behavior of nanostructured films based on NiTi shape memory alloys from the perspective of atomic scale. Full article

Machine learning was employed to make fluid agnostic laminar heat transfer prediction in supercritical conditions, encompassing three fluids (sCO₂, sH₂O, sC₁₀H₂₂) representing a wide range of operating conditions. High-fidelity training data, consisting of both non-dimensional and dimensional (operating parameter) as inputs and Nu and T_wall as outputs, were generated from grid-converged, steady-state, computational fluid dynamic (CFD) simulations. The Random Forest (RF) algorithm outperformed the artificial neural networks (ANNs) across all scenarios on the small multi-fluid dataset (~1600 data points) employed during the training process. When using non-dimensional parameters as inputs, Nu prediction fidelities were better than T_wall predictions for both ML algorithms across both horizontal and vertical configurations. The RF model trained on data from a specific flow configuration (horizontal/vertical) could predict T_wall within an accuracy of +/−1% with dimensional, operational parameters as inputs while being agnostic to the working fluid. Furthermore, by including the gravity vector as an additional variable during the training process, the RF model could predict T_wall accurately in a mixed, multi-fluid dataset containing data from both horizontal and vertical configurations. Full article

Horizontal transfer of mitochondrial DNA into the nuclear genome generates nuclear mitochondrial sequences (NUMTs), which serve as molecular fossils reflecting long-term mitochondrial–nuclear interactions and genome evolution. However, the biological mechanisms governing NUMT integration, retention, and evolutionary fate remain incompletely understood in domesticated animals. Here, using the latest pig reference genome assembly (Sscrofa11.1), we present a comprehensive genome-wide characterization of NUMTs in pigs and provide new insights into their genomic distribution and evolutionary constraints. We identified 513 high-confidence NUMTs, of which 460 were chromosomally mapped, accounting for 0.0106% of the nuclear genome. Beyond increased detection, our analyses reveal that pig NUMTs exhibit non-random origins, preferentially integrate into genomic regions under weak selective constraint, and are frequently associated with repetitive elements, consistent with a DNA repair-mediated insertion mechanism. NUMTs predominantly occur as short, fragmented sequences and show signatures of long-term neutral evolution, while insertions disrupting coding sequences are strongly selected against. Synteny-based analyses further identified clustered NUMT regions and duplicated NUMTs, suggesting secondary genomic duplication events following initial integration. Comparative analysis with the earlier Sscrofa10.2 assembly demonstrates that improved genome quality substantially enhances NUMT detection, particularly in repetitive and GC-rich regions, clarifying previously ambiguous sequence-context associations. Together, this high-quality pig NUMT map provides a robust foundation for future functional, evolutionary, and population-level investigations and contributes to the conservation and utilization of pig genetic resources. Full article

Background: Tumor-derived small extracellular vesicles (sEV), which we call TEX, carry a cargo of molecules that resembles the producer tumor cells. Circulating freely in body fluids, TEX potentially serve as a liquid tumor biopsy. TEX horizontally transfer their cargo to various recipient cells, imparting to them pro-tumor activity. Mechanisms of TEX-driven reprogramming might involve nucleic acids, especially double-stranded (ds)DNA. Methods: TEX isolated from supernatants of human tumor cells were identified as sEV, based on their size, endocytic origin and morphology. TEX treated with DNase/RNase cocktail were examined by transmission and cryo-electron microscopy and tested for biologic activity. DNA was extracted from enzyme-treated TEX, quantified by Qubit and analyzed for fragment sizes. The presence of genomic DNA in TEX was confirmed by PCR, and sequencing of the TP53 gene fragment for a mutational signature was performed. Results: Enzymatic and microscopic studies of TEX showed that nucleic acids are present in the biocorona on the outer surface. Their removal interfered with the biocorona integrity. A short TEX exposure to DNase/RNase altered their morphology without impairing vesicle functions; longer treatments induced TEX re-organization into smaller membrane-bound vesicles. The TEX lumen contained long fragments of protected genomic DNA with a mutational signature reflecting that of the tumor. Conclusions: Nucleic acids present on the TEX surface support the vesicular integrity. The TEX lumen contains membrane-protected large (ds)DNA fragments with the mutational signature of the parent tumor. The presence of surface and luminal nucleic acids in TEX, and especially their mutational signature, suggests that TEX may serve as highly promising cancer-specific biomarkers. Full article

Synanthropic cockroaches, especially Blattella germanica and Periplaneta americana, are persistent pests of human dwellings, healthcare facilities, food establishments, farms, and transport infrastructure. Accumulating field and laboratory studies indicate that synanthropic cockroaches carry clinically important bacteria, fungi, and parasites, including multidrug-resistant strains harbouring extended-spectrum β-lactamase, carbapenemase, and other antimicrobial-resistant determinants. Cockroaches acquire these organisms from sewage, waste, food residues, animal excreta, and contaminated clinical environments, and retain them on the cuticle and within a complex gut microbiota. Dissemination is predominantly mechanical, via contact transfer and deposition of regurgitate and faeces on food, equipment, and surfaces, but may be amplified by gut colonisation, microbial interactions, and horizontal gene transfer within the cockroach microbiome. In hospitals, cockroaches can connect high-burden reservoirs (drains, waste areas, kitchens) with vulnerable units, including intensive care units (ICUs), neonatal intensive care units (NICUs), burn units, and haemato-oncology wards. In food and livestock systems, they may contaminate housing, ingredients, and finished products, enabling spillover along supply chains and at ports. This review synthesises current evidence and highlights the following priorities: integrate cockroaches into infection prevention, food safety, and biosecurity; incorporate cockroach sampling into antimicrobial resistance (AMR) and genomic surveillance; and advance mechanistic research on cockroach–microbiota–pathogen interactions to improve pest management and safely explore cockroach-derived antimicrobial compounds. In this review, we distinguish external mechanical carriage (cuticular contamination) from internal gut carriage; we use “gut colonisation” only when persistence/replication or prolonged shedding is demonstrated. Full article