Search Results (48)

This study aimed to investigate the underlying mechanisms by which dandelion extract inhibits the proliferation of breast cancer MDA-MB-231 cells. Dandelion root and leaf extracts were prepared using a heat reflux method and subjected to solvent gradient extraction to obtain fractions with different polarities. MTT assays revealed that the ethyl acetate fraction exhibited the strongest inhibitory effect on cell proliferation. LC-MS analysis identified 12 potential active compounds, including sesquiterpenes such as Isoalantolactone and Artemisinin, which showed significantly lower toxicity toward normal mammary epithelial MCF-10A cells compared to tumor cells (p < 0.01). Mechanistic studies demonstrated that the extract induced apoptosis in a dose-dependent manner, with an apoptosis rate as high as 85.04%, and significantly arrested the cell cycle at the S and G2/M phases. Label-free quantitative proteomics identified 137 differentially expressed proteins (|FC| > 2, p < 0.05). GO enrichment analysis indicated that these proteins were mainly involved in cell cycle regulation and apoptosis. KEGG pathway analysis revealed that the antitumor effects were primarily mediated through the regulation of PI3K-Akt (hsa04151), JAK-STAT (hsa04630), and PPAR (hsa03320) signaling pathways. Moreover, differential proteins such as PI3K, AKT1S1, SIRT6, JAK1, SCD, STAT3, CASP8, STAT2, STAT6, and PAK1 showed strong correlation with the core components of the EA-2 fraction of dandelion. Molecular docking results demonstrated that these active compounds exhibited strong binding affinities with key target proteins such as PI3K and JAK1 (binding energy < −5.0 kcal/mol). This study elucidates the multi-target, multi-pathway synergistic mechanisms by which dandelion extract inhibits breast cancer, providing a theoretical basis for the development of novel antitumor agents. Full article

Calenduloside E (CE) and chikusetsusaponin IVa (ChIVa) are triterpene saponins with multidirectional bioactivity. In this study, the contents of CE and ChIVa were determined in the roots, stems, leaves, and fruits of ten wild-growing species of Amaranthaceae. To achieve optimal extraction conditions for both saponins, maceration, shaking-assisted maceration, and ultrasound-assisted and heat reflux extraction were compared. A sensitive, specific, and rapid UPLC-MS/MS method was developed and validated for the simultaneous quantification of CE and ChIVa. The results showed that CE and ChIVa coexisted in most of the species analyzed, except for Ch. hybridum. For the first time, the presence of CE and ChIVa was noted in L. polysperma, A. patula, B. bonus-henricus, O. rubra, and O. glauca. Of the species analyzed, the highest ChIVa content was found in the fruit of A. sagittata (13.15 mg/g dw), L. polysperma (12.20 mg/g dw), and Ch. album (10.0 mg/g dw), and in the fruit and roots of Ch. strictum (5.52 and 7.77 mg/g dw, respectively). The highest amount of CE was determined in the fruit of A. sagittata (7.84 mg/g dw) and Ch. strictum (6.54 mg/g dw). These saponin-abundant plant parts of Amaranthaceae spp. may be considered convenient sources of these bioactive saponins. Full article

Prunus mume (Armeniaca mume Sieb. var. mume f. viridicalyx (Makino) T. Y. Chen) is a common landscape plant. It has rich flavonoid content, which shows strong biological activities. In this study, the application potential of flavonoids in Prunus mume in anti-photoaging cosmetics was developed. Firstly, the flavonoids in Prunus mume were extracted by the heating reflux method, and the total flavonoid content reached 192 mg/g under the optimal process conditions determined by orthogonal testing. Then, the crude extract (PMCE) was purified by NKA-II resin column chromatography, and the purity of the flavonoids reached 83.99%. Subsequently, the Prunus mume purified product (PMPP) was analyzed by UHPLC-DAD-QTOF-MS/MS and 45 compounds were identified including flavonoid glycosides, phenolic acids and p-coumaroyl acetylated sucrose. In addition, in vitro biological experiments showed that PMPP had significant repair effects on UVB-induced photoaging of human keratinocytes (HaCaT), lowering reactive oxygen species (ROS) and malondialdehyde (MDA) levels, restoring superoxide dismutase (SOD) activity, and promoting hyaluronic acid (HA) secretion and Aquaporins-3 (AQP-3) levels. The results laid the foundation for the development of the flavonoids in Prunus mume as materials for anti-photoaging cosmetics. Full article

Geum japonicum Thunb. var. Chinese F. Bolle, a traditional Miao medicine with significant clinical potential, is rich in polysaccharides. Despite its importance, there is a scarcity of research on the structure and activities of these polysaccharides. In this study, polysaccharides from Geum japonicum (GJPs) were extracted using various methods, including heated reflux extraction (HRE), acidic extraction (ACE), alkaline extraction (AAE), microwave-assisted extraction (MAE), enzymatic extraction (EAE), pressurized liquid extraction (PLE), and deep eutectic solvents extraction (DESE). The extraction yield, physicochemical properties, structural characteristics, and antioxidant activities of these polysaccharides were comprehensively investigated and compared. Physicochemical analysis, including FT-IR spectral features and monosaccharide compositions, revealed that the GJPs are acidic heteropolysaccharides with both α- and β-configurations. DESE and ACE were the most effective methods for obtaining the highest neutral and acidic sugars with yields of 29.1%/64.2%, and 39.8%/55.6%, respectively. Meanwhile, AAE was preferable for extracting the polysaccharide–protein complex, achieving a yield of 14.21% and exhibiting superior thermal stability. In particular, DESE and PLE showed the best homogeneity with distinct molecular weights of 39.5 kDa and 17.6 kDa, respectively. In addition, biological evaluation indicated that DESE and MAE exhibited relatively stronger antioxidant activities as evidenced by DPPH and ABTS assays. Conversely, ACE demonstrated highest Fe²⁺ chelating ability but the lowest activity in DPPH and ABTS assays. Furthermore, the results of correlation analysis showed that the monosaccharides composition, protein and polyphenol content were significantly associated with the antioxidant activity. The choice of extraction method greatly affects the property and activity of G. japonicum polysaccharides. Polysaccharides extracted by deep eutectic solvents from G. japonicum show promise as natural antioxidants in the food and medicine industries. Full article

In the context of a circular economy, this study explores the valorization of blueberry pomace (BP) as a source of bioactive compounds using sustainable extraction methods. Microwave-assisted extraction (MAE) and microwave-assisted subcritical water extraction (MASWE) were employed to obtain two distinct fractions: MAE 1° and MASWE 2°. The first extract, MAE 1°, obtained at 80 °C, had a high total anthocyanin content (21.96 mg_Cya-3-glu/g_extract), making it suitable as a natural pigment. Additionally, MAE 1° exhibited significant enzyme inhibition, particularly against α-amylase and β-glucosidase, suggesting potential anti-diabetic and anti-viral applications. The second extract, MASWE 2°, obtained at 150 °C, contained a higher total phenolic content (211.73 mg_GAE/g_extract) and demonstrated stronger antioxidant activity. MASWE 2° showed greater inhibition of acetylcholinesterase and tyrosinase, indicating its potential for use in Alzheimer’s treatment, skincare, or as a food preservative. MASWE 2° exhibited cytotoxicity against HeLa cells and effectively mitigated H₂O₂-induced oxidative stress in HaCat cells, with MAE 1° showing similar but less pronounced effects. A tested formulation combining MAE 1° and MASWE 2° extracts in a 3:2 ratio effectively enhanced anthocyanin stability, demonstrating its potential as a heat-stable pigment. The extract characteristics were compared with a conventional method (MeOH-HCl in reflux condition), and the protocol’s sustainability was assessed using several green metric tools, which provided insights into its environmental impact and efficiency. Full article

Propolis has gained popularity in recent years as a potential preventive and therapeutic agent due to its numerous health benefits, which include immune system boosting, blood pressure lowering, allergy treatment, and skin disease treatment. The pharmacological activity of propolis is primarily attributed to phenolics and their interactions with other compounds. Given that phenols account for most of propolis’s biological activity, various extraction methods are being developed. The resin–wax composition of the propolis matrix necessitates the development of an extraction procedure capable of breaking matrix–phenol bonds while maintaining phenol stability. Therefore, the aim of this study was to assess the stability of two major groups of phenolic compounds, flavonoids and phenolic acids, in propolis methanol/water 50/50 (v/v) extracts obtained after ultrasound-assisted extraction (USE) under different extraction parameters (extraction time and pH) and heat reflux extraction (HRE). The methodology involved varying the USE parameters, including extraction time (5, 10, and 15 min) and pH (2 and 7), followed by analysis using liquid chromatography–tandem mass spectrometry (LC-MS/MS) to quantify phenolic recoveries. Results revealed that benzoic acid and chlorogenic acid derivatives demonstrated excellent stability across all ultrasound extraction procedures. The recoveries of flavonoids were highly diverse, with luteolin, quercitrin, and hesperetin being the most stable. Overall, neutral pH improved flavonoid recovery, whereas phenolic acids remained more stable at pH = 2. The most important optimization parameter was USE time, and it was discovered that 15 min of ultrasound resulted in the best recoveries for most of the phenols tested, implying that phenols bind strongly to the propolis matrix and require ultrasound to break the bond. However, the high variability in phenol extraction and recovery after spiking the propolis sample shows that no single extraction method can produce the highest yield of all phenols tested. As a result, when working with a complex matrix like propolis, the extraction techniques and procedures for each phenol need to be optimized. Full article

This study aimed to investigate the antioxidant activity and antibacterial effect of total flavonoids from Persicaria hydropiper (L.) Spach (TFs-Ph) and to provide a theoretical basis for the development of drugs for the treatment of pathogenic Escherichia coli and Salmonella spp. of broiler origin. Firstly, the response surface optimization heating reflux method was used to extract TFs-Ph, and the effects of ethanol concentration, solid–liquid ratio, heating reflux time, heating reflux temperature, and number of extraction times on the extraction yield of TFs-Ph were analyzed to determine the optimal extraction conditions. The antioxidant activity of TFs-Ph was determined by measuring the scavenging ability against hydroxyl radicals (•OH), 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion (•O²⁻), and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The antibacterial effect of TFs-Ph was determined by the disk diffusion method. The results showed that the optimal extraction parameters of TFs-Ph were as follows: ethanol concentration of 51%, solid-liquid ratio of 1:24 g/mL, heating reflux time of 74 min, heating reflux temperature of 70 °C, and three extraction times; in this case, the extraction yield of TFs-Ph was 6.37%. TFs-Ph had a strong scavenging ability against the free radicals of •OH, DPPH, •O²⁻, and ABTS, and the antioxidant activity was better than that of vitamin C (Vc). In addition, it showed a better antibacterial effect against pathogenic Escherichia coli and Salmonella of broiler origin compared with ampicillin (AMP). Therefore, TFs-Ph have a certain potential to replace antibiotics. Full article

The ultrasonic-assisted extraction (UAE) method was employed to separate Cinnamomum camphora proanthocyanidin-rich extracts (PCEs). This extraction process was optimized by the Box–Behnken design, and the optimal conditions, on a laboratory scale, were as follows: an ethanol concentration of 75%, a liquid-to-solid ratio of 24 mL/g, an ultrasonic time of 39 min, and an ultrasonic power of 540 W. Under the obtained conditions, the PCE yield extracted by UAE was higher than that from heat reflux extraction and soaking extraction. An ultra-performance liquid chromatography–tandem mass spectrometry analysis was employed to characterize the phloroglucinolysis products of the C. camphora PCEs, by which epigallocatechin, catechin, epicatechin, and (−)-epigallocatechin-3-O-gallate were identified as the terminal units; epigallocatechin, epicatechin, and (−)-epigallocatechin-3-O-gallate were recognized as extension units. The C. camphora PCEs possessed higher anti-ultraviolet activity in vitro compared with the commercially available sunscreen additive of benzophenone with respect to their ethanol solutions (sun protection factor of 27.01 ± 0.68 versus 1.96 ± 0.07 at a concentration of 0.09 mg/mL) and sunscreens (sun protection factor of 17.36 ± 0.62 versus 14.55 ± 0.47 at a concentration of 20%). These results demonstrate that C. camphora PCEs possess an excellent ultraviolet-protection ability and are promising green sunscreen additives that can replace commercial additives. Full article

Rosmarinus officinalis leaves (ROLs) are widely used as a popular culinary spice for flavoring food, in which carnosic acid (CA) and rosmarinic acid (RA) are the main active components. The extraction of CA and RA is limited by lowextraction efficiency and extraction rate. In this work, a microwave-assisted extraction (MAE) method using biodegradable, low-toxic and nonflammable solvents polyethylene glycols (PEGs) as extraction solvents was developed for theextraction of CA and RA from ROLs. Experimental results suggest that PEG-400 was a better choice compared with PEG-200, and the optimal extraction conditions were as follows: 45% of PEG-400, 4.3% of phosphoric acid, 20 s of microwave irradiation time at 280 W of microwave irradiation power, and a 10 mg mL⁻¹ solid–liquid ratio, respectively. The tissue structures of ROLs could be effectively disrupted by PEG-based MAE, leading to high CA and RA extraction efficiencies. The PEG-400 extract exhibited stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability compared with butylated hydroxytoluene (BHT). Finally, compared with heating reflux extraction, ultrasound-assisted extraction, maceration, and MAE using ionic liquid and ethanol as extraction solvents, the developed PEG-400 based MAE exhibited the highest extraction ability and fastest extraction rate for CA and RA. These findings suggest that MAE using PEGs as extraction solvents is a promising method for the separation of bioactive compounds from natural plants. Full article

This study presents the analysis of the exergy load distribution in a separation process by extractive distillation for ethanol dehydration. The methodology carried out is divided into three parts: the calculation of the flow exergy considering the physical and chemical exergies of the distillation process; the calculation of the primary and transformed exergy contributions considering the consumed exergy; and finally, the overall process efficiency, which shows the real percentage of energy being used in the process. The simulation of an extractive distillation separation system is carried out using Aspen Plus^®, from Aspen Tech Version 9. In general, heat transfer processes (heating or cooling) are the ones that generate the greatest exegetic destruction, which is why they must be the operations that must be optimized. As a result of our case study, the local exergy efficiency of the extractive distillation column is 13.80%, which is the operation with the greatest energy loss, and the overall exergy efficiency of the separation system is 30.67%. Then, in order to increase exergy efficiency, a sensitivity analysis is performed with the variation of the azeotrope feed, number of stages, reflux ratio, and solvent feed variation on ethanol purity to reach an overall efficiency of 33.53%. The purity of ethanol is classified as higher than that of the specified, 99.65%. Full article

Corrosion is the process of damaging materials, and corrosion of metallic materials frequently results in serious consequences. The addition of corrosion inhibitors is the most effective means of preventing metal corrosion. Until now, researchers have made unremitting efforts in the research of high-efficiency green corrosion inhibitors, and research on biomass corrosion inhibitors in a class of environmentally friendly corrosion inhibitors is currently quite promising. This work presents the classification of green biomass corrosion inhibitors in detail, including plant-based corrosion inhibitors, amino acid corrosion inhibitors, and biosurfactant corrosion inhibitors, based on the advantages of easy preparation, environmental friendliness, high corrosion inhibition efficiency, and a wide application range of biomass corrosion inhibitors. This work also introduces the preparation methods of biomass corrosion inhibitors, including hydrolysis, enzymatic digestion, the heating reflux method, and microwave extraction. In addition, the corrosion inhibition mechanisms of green biomass corrosion inhibitors, including physical adsorption, chemisorption, and film-forming adsorption, and evaluation methods of biomass corrosion inhibitors are also explicitly described. This study provides valuable insights into the development of green corrosion inhibitors. Full article

The fruit of Phaleria macrocarpa have been traditionally used as an antidiabetic remedy in Malaysia and neighbouring countries. Despite its potential for diabetes treatment, no scientific study has ever been conducted to predict the inhibitor interaction of the protein α-glucosidase identified in an extract prepared with a non-conventional extraction technique. Hence, the major aim of this research was to evaluate the in vitro antioxidant, the α-glucosidase inhibitors, and the molecular dynamic simulations of the α-glucosidase inhibitors identified by Quadrupole Time-of-Flight Liquid Chromatography Mass Spectrometry (Q-ToF-LCMS) analysis. Initially, dry fruit were processed using non-conventional and conventional extraction methods to obtain subcritical carbon dioxide extracts (SCE-1 and SCE-2) and heating under reflux extract (HRE), respectively. Subsequently, all extracts were evaluated for their in vitro antioxidative and α-glucosidase inhibitory potentials. Subsequently, the most bioactive extract (SCE-2) was subjected to Q-ToF-LCMS analysis to confirm the presence of α-glucosidase inhibitors, which were then analysed through molecular dynamic simulations and network pharmacology approaches to confirm their possible mechanism of action. The highest inhibitory effects of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and α-glucosidase on SCE-2 was found as 75.36 ± 0.82% and 81.79 ± 0.82%, respectively, compared to the SCE-1 and HRE samples. The Q-ToF-LCMS analysis tentatively identified 14 potent α-glucosidase inhibitors. Finally, five identified compounds, viz., lupenone, swertianolin, m-coumaric acid, pantothenic acid, and 8-C-glucopyranosyleriodictylol displayed significant stability, compactness, stronger protein-ligand interaction up to 100 ns further confirming their potential as α-glucosidase inhibitors. Consequently, it was concluded that the SCE-2 possesses a strong α-glucosidase inhibitory effect due to the presence of these compounds. The findings of this study might prove useful to develop these compounds as alternative safe α-glucosidase inhibitors to manage diabetes more effectively. Full article

Plant extracts have been widely used for skin care for many centuries, and nowadays, they are commonly applied for the development and enrichment of new cosmetic preparations. The present study aimed the assessment of the biological activity of aqueous Schisandra chinensis extracts as a potential ingredient of skin care products. The aspects studied involved the ability to neutralize free radicals, impact on viability and metabolism of keratinocytes, as well as tyrosinase inhibitory potential. Our study showed that aqueous S. chinensis extracts have a positive effect on keratinocyte growth and have high antioxidant potential and strong tyrosinase inhibitory activity. UPLC-MS analysis revealed that three groups of phenolic compounds were predominant in the analyzed extract, including lignans, phenolic acids and flavonoids and protocatechiuc and p-coumaryl quinic acids were predominant. Moreover, microwave-assisted extraction, followed by heat reflux extraction, was the most effective for extracting polyphenols. Furthermore, a prototypical natural body washes gel formulation containing the previously prepared extracts was developed. The irritation potential and viscosity were assessed for each of the formulations. The study demonstrated that the addition of these extracts to body wash gel formulations has a positive effect on their quality and may contribute to a decrease in skin irritation. In summary, S. chinensis aqueous extracts can be seen as an innovative ingredient useful in the cosmetic and pharmaceutical industry. Full article

Rapanone is a plant-derived simple alkyl-dihydroxybenzoquinone structurally close to embelin, a well-known cytotoxic agent. The pharmacological characterization of rapanone is still incomplete, and to fill the data gap, a good source for its acquisition is required to conduct further research. This study aimed to optimize the conditions for the extraction of rapanone from the leaves of white-berried Ardisia crenata Sims. For this purpose, three methods were employed: heat reflux (HRE), shaking (SE), and ultrasound-assisted extraction (UAE), and such parameters as the extraction time, solvent, and the number of extractions from the same sample were set as experimental variables. Furthermore, cytotoxic activity toward prostate cancer, thyroid cancer, and colorectal carcinoma cell lines was investigated and compared with doxorubicin and embelin. The most effective and economical method for the extraction of rapanone was shown to be 20 min UAE with ethyl acetate or chloroform. Rapanone exhibited high cytotoxic activity against PC3 (IC₅₀ = 6.50 μg/mL), Du145 (IC₅₀ = 7.68 μg/mL), FTC133 (IC₅₀ = 6.01 μg/mL), 8505C (IC₅₀ = 7.84 μg/mL), and Caco-2 (IC₅₀ = 8.79 μg/mL) cell lines after 24 h and against the HT29 cell line after 48 h (IC₅₀ = 11.67 μg/mL). Furthermore, it revealed a more favorable safety profile than either its homologue, embelin, or doxorubicin. The set of optimal extraction parameters obtained may be utilized for scientific and industrial purposes to achieve the best rapanone yield. Moreover, this benzoquinone revealed a high cytotoxic activity with good selectivity. Full article

The aim of this study was to use plant extracts from spontaneous flora of Moldova (Rediu-Iasi area, Romania) as polyphenols and flavonoids source in order to obtain new dermato-cosmetic formulas to prevent the actions of oxidative stress on skin. Plant extracts (from raw and dried Galium verum sp.) were obtained by: cold classical maceration (M), heat reflux extraction (HTE) and ultrasound assisted extraction (UAE). The extracts were characterized by spectrophotometric method (for polyphenols and flavonoids content and for DPPH antioxidant activity evaluation). In order to evaluate the combating and/or attenuating effects of oxidative stress on skin, the study was continued with the elaboration of emulsions that incorporate one of these extracts. The emulsions were preliminarily characterized by determining the stability over time. The obtained results encourage research in the direction of deeper characterization of these emulsions to determine the microbiological stability and dermatological tests performed on the skin treated with these new products. Full article