Search Results (4,472)

Background: Myocarditis is an inflammatory disease of the myocardium with multiple causes and evolutions. The aim of our study was to design a prognostic multiparametric score in patients with myocarditis, to identify those at higher risk of cardiovascular outcomes. Methods: A prospective study was performed enrolling 98 patients with myocarditis: 72 M, 26 F; median age 27 [IQR 20–40]. Patients were divided into two groups: complicated (CM) and uncomplicated myocarditis (UM). Six months after hospital admission, cardiac magnetic resonance (CMR) and cardiological consultation were repeated. Cardiovascular outcomes (death, hospitalization for heart failure, heart transplant, ICD implantation, and heart failure development) were evaluated at 6 months and after 3 years. Results: We found 67 UM and 31 CM. Cardiovascular outcomes were significantly higher in patients with CM. We found a significant correlation between cardiovascular outcomes and reduced LVEF at hospital admission, reduced global longitudinal strain in absolute values, septal late gadolinium enhancement (LGE) at CMR, longer persistence time of increased troponin, LGE extension progression or persistence at 6 months of CMR. A myocarditis prognostic score was developed. A score ≥ 5 showed higher sensitivity (100%) and specificity (87%)—AUC 1, to identify cardiovascular outcomes in patients with myocarditis. A score between 3 and 4 showed high sensitivity but low specificity. A score ≤ 2 was associated with low probability of cardiovascular outcomes. Conclusion: Our study confirms the high probability of cardiovascular outcomes in patients with CM and it suggests a myocarditis prognostic score to identify patients at higher risk of cardiovascular outcomes. Full article

Rural land art festivals serve as an important practical vehicle for integrating urban and rural culture and tourism. They constitute a crucial component of rural tourism in China and play a key role in the sustainable development of rural areas. However, in practice, these festivals are generally confronted with the dilemma of superficial tourist participation and insufficient sustainability. This study aims to uncover the intrinsic psychological evolution mechanism underlying tourists’ responses to external stimuli and their value co-creation. The S-O-R model and the two-factor theory are integrated to construct an analytical framework: “external stimulus–psychological sequence–behavioral response.” Using “Modern Fields” as the case study and 437 valid data points, an empirical analysis is conducted with PLS-SEM and artificial neural networks (ANNs). The results indicate that tourist participation is directly driven by destination quality. Content stickiness exerts an indirect influence through perceived value. Perceived value facilitates value co-creation only when it is fully mediated by tourist participation. The path from participation to co-creation is significantly strengthened by restorative environmental perception. A multi-group analysis further reveals that inexperienced tourists exhibit a “stimulus-driven” characteristic, whereas experienced tourists follow a “value internalization” path. The ANN analysis further shows that the strongest nonlinear predictive power for co-creation behavior is held by restorative environmental perception. A significant direct nonlinear effect is also exerted by destination quality. The evolutionary nodes and boundary conditions of tourists’ psychological sequence during this process are revealed. The boundary effect of restorative environmental perception as a catalyst for rural art tourism is demonstrated. A theoretical basis and practical insights are thereby provided for the segmented operation and sustainable development of these activities. Full article

Magnesium phosphate cement (MPC) shows great potential for complex underground environments due to its rapid-hardening and early-strength properties. However, its large-scale application is hindered by several drawbacks, including high hydration heat, rapid setting, and insufficient long-term durability. To address these limitations, this study developed a novel MPC grouting material modified with steel slag (SS) and redispersible latex powder (LP). We systematically investigated the workability, mechanical properties, durability, and microstructural evolution of this modified system. Results indicate that incorporating SS and LP decreases both the fluidity and setting time of the grout. An optimal SS dosage accelerates reaction kinetics and raises the peak hydration temperature. Conversely, the LP-induced polymer film suppresses the overall temperature rise, delaying the first exothermic peak and advancing the second. The incorporation of 5% steel slag increased the 28-day compressive strength of the MPC to 54.86 MPa. Building on this, the combined addition of 0.15% latex powder further elevated the strength to 58.82 MPa. Microstructural and pore analyses confirmed that the steel slag enhanced interfacial bonding through physical filling and the formation of calcium phosphate crystals. Meanwhile, the latex powder formed a continuous polymer film, which tightly wrapped and bridged the hydration products and unreacted particles. This synergistic mechanism effectively sealed the capillary pores and reduced the proportion of harmful pores by 15.99% compared to the control group. Consequently, the densified MPC matrix laid a solid microstructural foundation for the material’s excellent durability. It offers reliable, high-performance material for seepage control and strata reinforcement in complex environments. Full article

Composed of a steroid nucleus, widely distributed in the animal and plant kingdoms, containing various hydroxyl and methyl groups, and a carboxyl side chain, bile acids (BAs) appear to be the result of an irreversible evolution in nature. BAs are involved in numerous vital processes, such as enterohepatic circulation, recognition and transport by various proteins, and their role as “clients” of the farnesoid X receptor, suggesting that they could be used as carriers, transporters, or Trojan horses to deliver a drug to its target. Pioneers of this approach include Ehrlich, Ho, and Kramer, who conceived of “magic bullet” concepts and designed what are now known as conjugated BAs or drug–BA complexes. This review focuses on articles that apply these concepts to the broad and complex field of cancer research. Most of the reviewed studies follow a common trajectory encompassing the design and synthesis of BA conjugates, the in vitro evaluation of their anticancer activity in various cell lines, and their subsequent in vivo assessment. More than 250 compounds have been taken into consideration. Full article

Optical fiber sensors deployed in harsh industrial fields, e.g., high-temperature wet-oxygen, face severe challenges in signal attenuation and mechanical degradation. While amorphous silicon oxycarbide (a-SiOC) coatings offer a promising solution due to their adjustable thermo-mechanical properties, balancing their structural density with environmental stability remains a critical technical bottleneck. In this study, a-SiOC coatings were deposited on optical fibers using hexamethyldisilane (HMDS) and trace oxygen via radio-frequency capacitively coupled plasma-enhanced chemical vapor deposition (PECVD). A systematic investigation was conducted to determine the impact of deposition temperature (70–420 °C) on the precursor dissociation kinetics, microstructural evolution, and corrosion resistance of the coatings. An elevation in temperature promotes the elimination of organic terminal groups (–CH₃, –H) and enhances surface diffusion, driving the coating from a loose, carbon-rich “polymer-like” structure (dominated by Si–C bonds) to a dense, inorganic “silica-like” skeleton (dominated by Si–O–Si bonds). High-temperature corrosion tests in a wet-oxygen environment (500–900 °C) demonstrate that the failure mechanism is highly dependent on deposition temperature. Coatings deposited at low temperatures suffer catastrophic cracking due to pronounced oxidative shrinkage and the release of volatile species, whereas coatings deposited at 420 °C exhibit microcracking caused by severe carbon phase separation and stress concentration within the rigid inorganic network. In the present system, 350 °C is identified as the optimal deposition temperature, as it achieves the best balance of network densification and structural flexibility, while exhibiting the best mechanical performance. Full article

Background: Relapse is the leading cause of treatment failure in patients with myeloid hematologic malignancies undergoing allogeneic hematopoietic cell transplantation. Clonal genomic evolution may contribute to post-transplant relapse, yet its determinants and prognostic impact remain incompletely characterized. Methods: In this retrospective study, we analyzed 63 patients with myeloid neoplasms who underwent cytogenetic evaluation both at diagnosis and at relapse after allogeneic hematopoietic stem cell transplantation. Cytogenetic changes (CGE), including evolution, devolution, or combined patterns, were assessed and correlated with clinical characteristics, prior treatment exposure, and survival outcomes. Results: Cytogenetic changes were observed in 46.1% of patients. The presence of cytogenetic changes (CGE) was strongly associated with the presence and complexity of cytogenetic abnormalities at initial diagnosis, whereas prior chemotherapy exposure, conditioning intensity, and donor type showed no significant association. Patients with cytogenetic changes had a lower complete remission rate at day 30 after transplantation; however, relapse-free survival and post-relapse survival did not differ significantly between groups. Conclusions: These findings suggest a potential association between post-transplant cytogenetic changes and intrinsic genomic instability, although treatment-related effects cannot be excluded. Larger, disease-stratified studies integrating cytogenetic and molecular analyses are warranted to further clarify the biological and prognostic relevance of clonal evolution following transplantation. Full article

Seeds are the result of sexual reproduction, containing the embryo that stores genetic information from past generations, surrounded by protective and nutritional tissues. In Vitis, seed morphology provides valuable insights into varietal diversity and domestication processes. In the context of transitioning toward sustainable viticulture, understanding varietal diversity provides key insights into crop evolution and adaptation. This study explores relationships in seed morphology among 91 varieties conserved in the Portuguese Ampelographic Collection (PRT 051 in FAO). Based on images of well-oriented seeds, outline geometry was described using Fourier coefficients and curvature values measured at key points along the outline. Seeds were classified according to their similarity to four reference models: Sylvestris, Hebén, Traminer, and Koenigin der Weingaerten. A high proportion of cultivars showed strong similarity to the Koenigin der Weingaerten model, suggesting an advanced stage of domestication. In contrast, very few cultivars matched the Sylvestris model. Significant differences in curvature values were observed among groups. The results confirm known pedigree relationships and the key role played by ancient varieties and provide new insights into the evolution of seed morphology during grapevine domestication. Among cultivars resembling the Koenigin der Weingaerten model, some result from crosses involving Iberian and European varieties, suggesting that the different Vitis haplotypes may be associated with progressive stages during the process of domestication that define the current resilience of Mediterranean grapevines. Full article

Life demonstrates remarkable homochirality of its major building blocks: nucleic acids, amino acids, sugars, and phospholipids. Phospholipid bilayer vesicles (liposomes) are formed at the water/air interface from Langmuir layers and contain ribose, a constituent of primordial water. Although the primordial ribose was initially racemic, life, as we know it, is homochiral, with d-ribose and its derivatives as the predominant forms. The phospholipid membrane’s permeability to d-ribose, together with ribose’s interaction with the bilayer’s charged phosphate groups, leads to ribose phosphorylation, yielding d-ribose-5-phosphate. Once inside, the d-ribose-5-phosphate molecules cannot cross the membrane. A similar path also exists for l-ribose, but with a lower rate. Therefore, overall, this process is enantioselective, favoring the buildup of d-ribose over l-ribose. Through liposome fusion, fission, and self-replication, this eventually leads to the Darwinian evolution of these structures and to the conversion of d-ribose-5-phosphate into complex functional molecules, such as ribozymes and RNA, and eventually into DNA, all of which inherit d-ribose’s chirality. Full article

Background: Nutritional literacy is a core competency for promoting healthy dietary behaviors and preventing nutrition-related chronic diseases. Standardized scales are essential for rigorous measurement and evaluation, yet the field exhibits substantial heterogeneity in concepts and measurement approaches. Methods: We systematically searched five major databases, namely Web of Science, PubMed, Scopus, Embase, and CINAHL, from their inception to October 2025. Evidence was compiled on the conceptual evolution, domain structure, scoring logic, population-specific applicability, and application scenarios of nutritional literacy scales. Results: A total of 14 nutritional literacy scales developed between 2005 and 2025 were included in the review. The structure and measurement content of these scales have progressively expanded, evolving from an early focus on basic reading and numeracy skills to become multidimensional assessment tools encompassing knowledge, skills, and behavioral practices. The target population has broadened from the general adult population to include multiple special groups, while application regions have extended from high-income Western countries to developing regions, including China and Turkey, and assessment methodologies have progressively shifted from single tests to blended objective–subjective approaches, with most scales demonstrating sound reliability and validity. These instruments are now employed for screening, intervention evaluation, dietary behavior mechanism research, and analysis of chronic disease risk. The reviewed studies indicate that nutritional literacy is generally positively correlated with healthy dietary behaviors, nutrition labeling utilization, and related health outcomes. Conclusions: Although nutritional literacy scale research has advanced with regard to conceptualization, measurement design, and applications, major gaps remain, including fragmented dimensional structures, insufficient standardization, inadequate cultural adaptation, and limited longitudinal evidence. Future work should prioritize a unified assessment framework, stronger tools for special and vulnerable populations, digital innovations for scalable measurement, and interdisciplinary and cross-national collaboration to enhance quality, practicality, and comparability and to support global nutrition promotion and public health policy. Full article

Glass fiber/epoxy (GF/EP) composites are widely used in high-voltage electrical equipment due to their excellent specific strength, durability and dielectric properties. However, long-term exposure to hygrothermal environments will lead to performance degradation of the material, which seriously threatens its service reliability. To solve this problem, accelerated aging tests were systematically carried out in this study by immersing GF/EP specimens in deionized water at room temperature and 80 °C. The performance evolution laws and failure mechanisms of the material were investigated through moisture absorption kinetic analysis, tensile property testing, scanning electron microscope (SEM) fracture observation and breakdown voltage testing. The results show that the initial moisture absorption behavior of the material follows the Fickian diffusion mechanism, and the water diffusion rate at 80 °C is 31.8 times that at room temperature. After 35 days of aging, the retention rate of the maximum tensile force is 86.6% for the room temperature group, while it decreases to 38.2% for the 80 °C group. SEM observations show that the failure mode of the material changes from ductile fracture to brittle fracture after aging at 80 °C, accompanied by serious interfacial debonding. Temperature is the dominant factor for insulation performance degradation: the breakdown voltage retention rate is above 91% at room temperature, while it decreases to about 37% at 80 °C, and the influence of 60% maximum tensile force ($F_{\mathrm{m}\mathrm{a}\mathrm{x}}$) preloading is relatively small. This study provides experimental data and theoretical support for the performance evaluation and life prediction of GF/EP composites in harsh hygrothermal service environments of high-voltage electrical equipment. Full article

In this study, La-doped TiO₂-SiO₂ composite films were deposited on glass substrates by radio-frequency magnetron sputtering. The evolution of microstructure and macroscopic properties was systematically investigated across an annealing temperature range of 350–650 °C. The results show that the La-doped TiO₂-SiO₂ composite structure effectively suppresses abnormal grain growth and delays the anatase-to-rutile phase transition, thereby improving the films’ high-temperature structural stability. Notably, the composite film annealed at 550 °C (LS-550) exhibits the highest anatase crystallinity and forms a dense, smooth (RMS = 1.37 nm), crack-free nanocrystalline network. In terms of wettability, the improved hydrophilicity is attributed to the combined effects of La incorporation and hydrophilic silanol (Si-OH) groups in the amorphous SiO₂ phase. As a result, the water contact angle of the LS-550 film decreases dramatically to 28.0°, indicating excellent hydrophilicity. Moreover, the LS-550 film demonstrates an optimal photocatalytic degradation efficiency of approximately 76% for methylene blue, significantly outperforming the pure TiO₂ film. Furthermore, the enhanced mechanical performance is associated with the combined effects of the SiO₂-containing amorphous phase and the finer microstructure induced by La incorporation. Consequently, the critical load (Lc) of the LS-550 film reaches 75.64 mN, significantly exceeding that of the pure TiO₂ film annealed at the same temperature (61.25 mN). In summary, the composite film annealed at 550 °C concurrently achieves high crystallographic thermal stability, robust interfacial mechanical durability, excellent surface hydrophilicity, and enhanced photocatalytic activity, thereby offering practical guidance for developing TiO₂-based coatings with self-cleaning potential for high-rise building curtain walls. Full article

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by immune dysregulation and multi-organ damage. Abnormal B cell activation and autoantibody production constitute the core pathological mechanism of SLE. However, the proportion, BCR pairing types, clonal evolution patterns, and transcriptomic features of dual BCR B cells in SLE remain incompletely elucidated. In this study, we employed single-cell RNA sequencing (scRNA-seq) combined with single-cell B cell receptor repertoire sequencing (scBCR-seq) to preliminarily analyze the proportion and characteristics of dual BCR B cells in SLE model mice (MRL/Lpr and SLE.Yaa) as well as in peripheral blood from SLE patients. The results showed: (1) Compared with control groups, the proportion of dual BCR B cells in SLE model mice and patients exhibited a decreasing trend, whereas the diversity of the CDR3 repertoire decreased and clonality increased. Increased clonal sharing was observed between single BCR B cells and dual BCR B cells. The main pairing types of dual BCR B cells were H + κ1 + κ2, H1 + H2 + κ, and H1 + H2 + κ + λ, with preferential utilization of autoimmunity-associated V gene families such as IGHV4-34, and high expression of IGHG subtypes. (2) Tracking analysis of B cell receptor clonality and effector molecule expression revealed that in SLE, dual BCR B cells tend to enrich in IFN-α/γ responses, TNF-NFκB inflammation, and complement pathways, and highly express interferon-related genes such as Ly6a, Isg15, MX1, and IFI6. (3) In both single BCR B and dual BCR B cells from SLE patients, the proportion of the naïve B cell subset decreased, whereas the proportions of plasma and Breg subsets increased and exhibited clonal expansion. SLE dual BCR Breg cells highly expressed IL10, HSPA1A, and others. This study is the first to reveal, at the high-throughput single-B-cell level, that the proportion, subset origin distribution, CDR3 repertoire composition, and effector molecule expression of dual BCR B cells display unique characteristics in SLE model mice and patients, providing baseline comparative data and novel research perspectives for further investigation into B cell effector functions and mechanisms in SLE patients. Full article

Single-phase Cu₄Bi₄Se₉ was successfully synthesized through a simple and rapid process combining mechanical alloying (MA) and hot pressing (HP). The phase formation behavior, microstructural evolution, charge transport characteristics, and thermoelectric properties were systematically investigated. X-ray diffraction analysis as a function of MA time confirmed that all powders crystallized into a single orthorhombic phase with space group Pnma. No decompositions or secondary phases were observed after HP sintering, indicating high phase stability. Thermogravimetric and differential scanning calorimetric analyses revealed distinct endothermic peaks at 714–717 K for all samples, corresponding to the onset of the decomposition of Cu₄Bi₄Se₉. Microstructural observations showed that the relative density decreased with increasing HP temperature (>573 K), accompanied by grain growth and pore formation, reflecting the competition between Cu–Se interdiffusion and pore coarsening during high-temperature sintering. Hall effect measurements indicated p-type conduction for all samples, with carrier concentrations on the order of 10¹⁷ cm⁻³ and carrier mobilities of approximately 10² cm² V⁻¹ s⁻¹. With increasing temperature, the electrical conductivity increased monotonically, while the Seebeck coefficient gradually decreased, resulting in a maximum power factor of 0.12 mW m⁻¹ K⁻² at 573 K. The total thermal conductivity remained extremely low, ranging from 0.33 to 0.48 W m⁻¹ K⁻¹, with the electronic contribution accounting for less than 10%, indicating that lattice thermal transport is dominant. The suppressed lattice thermal conductivity is attributed to the combined effects of Cu atomic rattling, asymmetric bonding induced by Bi 6s² lone-pair electrons, and strong anharmonic phonon scattering arising from the complex crystal structure. Consequently, Cu₄Bi₄Se₉ achieved a peak dimensionless figure of merit ZT of 0.19 in the temperature range of 573–623 K, demonstrating that the MA–HP process enables stable phase formation and competitive thermoelectric performance without post-annealing. Full article

The effects of RF plasma treatments using different gases (Ar, O₂, and N₂) and processing parameters on the surface wettability of polyethylene terephthalate (PET) films were systematically investigated. Atomic force microscopy (AFM) and X–ray photoelectron spectroscopy (XPS) were employed to characterize the evolution of surface topography and chemical composition. While all treatments enhanced hydrophilicity, the magnitude of improvement and the governing mechanisms were gas-dependent. Among them, O₂ plasma treatment exhibited the most pronounced effect: under optimal conditions (20 W, 80 s), the water contact angle (WCA) was reduced to 3.7°, indicating a superhydrophilic surface. This enhancement was primarily attributed to a substantial increase in surface oxygen content (O/C ratio) and the incorporation of strongly polar oxygen-containing functional groups, such as C=O and COOH. N₂ plasma offered moderate improvement via nitrogen-containing groups, while non-reactive Ar plasma relied primarily on physical etching, yielding the smallest enhancement. Analysis revealed that wettability evolution was dominated by increased polar surface energy from chemical functionalization, with surface roughness playing a synergistic role. These results demonstrate that optimizing plasma gas and parameters effectively controls PET wettability through the coupled regulation of surface chemistry and topography. Full article

Background: Diffuse large B-cell lymphoma (DLBCL) is one of the most frequent lymphomas. To date, it is not possible to identify which DLBCL patients will have an aggressive clinical evolution only by using hematoxylin and eosin (H&E) histological images. Methods: This study predicted the prognosis of DLBCL using H&E images, computer vision and deep learning. The series included 114 DLBCL cases, split into 2 prognostic groups according to overall survival, and 44 cases of reactive lymphoid tissue. Results: The curve fitting and slope analysis showed a point of inflection at 2 years (24 months), which differentiated patients with aggressive clinical evolution (“Dead < 2 years”, b1 = −0.024) from the rest with moderate clinical evolution (“Others”, b1 = −0.003). Twenty different convolutional neural networks (CNNs) were used, and explainable artificial intelligence (XAI) was also applied. The final model based on DarkNet-19 predicted prognosis groups with high performance (test set accuracy = 96.3%). The other performance parameters were precision (94.5%), recall (95.0%), false positive rate (3.1%), specificity (96.9%), and F1 score (94.7%). XAI, including grad-CAM, occlusion sensitivity, and image-LIME, confirmed that the CNN focused on the correct areas. Hybrid partitioning to prevent information leakage with patient-based analysis, image classification between DLBCL and 44 cases of reactive lymphoid tissue, and hyperparameter tuning were also successfully performed. Correlation with the clinicopathological characteristics found that the Dead < 2 years group was correlated with stage III–IV, International Prognostic Index (IPI) High + High/intermediate, progressive disease, non-GCB cell-of-origin, CD10−, BCL2+, and Epstein–Barr virus (EBER)+. Analysis of the microenvironment, immune checkpoint, cell cycle, and germinal center markers showed that Dead < 2 years had higher IL10, PD-L1, and CD163 levels and lower E2F1 protein expression. No differences were found for Ki67, CSF1R, CASP8, TNFAIP8, LMO2, MYC, MDM2, CDK6, and TP53 markers at a quantitative level. Conclusions: The DLBCL overall survival can be predicted using H&E histological images and deep learning using the 2-year (24 months) point (similar to POD24). This trained CNN can be used as a pretrained model for transfer learning in the future. Full article