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Search Results (3,219)

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40 pages, 6016 KB  
Review
Advanced Technologies to Treat Manure Generated on Dairy Farms: Overview and Perspectives for Intensifying Australian Systems
by Sharon R. Aarons, José A. D. López-Coronado, Scott McDonald and Rachael Campbell
Agriculture 2026, 16(7), 747; https://doi.org/10.3390/agriculture16070747 (registering DOI) - 27 Mar 2026
Abstract
Livestock production systems are considered some of the most environmentally degrading due to greenhouse gas (GHG) emissions and their contribution to poor air, soil and water quality, amongst other impacts. Advanced manure treatment technologies are required in response to intensification of dairy production [...] Read more.
Livestock production systems are considered some of the most environmentally degrading due to greenhouse gas (GHG) emissions and their contribution to poor air, soil and water quality, amongst other impacts. Advanced manure treatment technologies are required in response to intensification of dairy production worldwide, and the considerably greater volumes of manure generated that require collection and management. Similarly, in Australian dairy systems cows spend more time off pasture, with increased collection of larger manure volumes from a range of contained housing facilities. Adoption of advanced treatment is required to capture nutrients at risk of loss, and ideally to valorise manure to support uptake of these technologies. This review describes the generation of manure and the manure sources found in commercial Australian systems, including grazing-based and intensive dairy farms, supporting zero grazing. The review draws on manure data from pasture-based industries elsewhere and summarises their properties for comparison with Australian systems. Manure treatments that recover and retain nutrients, water and energy are reviewed. These include additives, mechanical/chemical/membrane separation, thermochemical and biological treatments which produce organic and inorganic soil amendments, clarified or potable water, gases (N2, H2), biofuels and energy. The review describes the technical and operational details of the technologies, and where there are opportunities for the Australian dairy industry. Treatment technologies need to be validated for Australian systems based on the collated data of local manure properties, as differences with international manure data have been observed. The relative costs, technological maturity, and the benefits and challenges associated with adoption are discussed. Many advanced technologies are ready for adoption, but others are experimental or at pilot stage and relative costs range from low to very high. However, to accurately assess feasibility of manure treatments, environmental, and production benefits should be balanced against capital and operating expenses and account for costs associated with current management. For large intensive farms, implementing advanced manure technologies may be required to ensure approval to operate/expand and to meet regulatory compliance. Future research for the Australian industry should investigate nutrient retention and further develop separation treatments incorporating chemical and mechanical technologies. Bioconversion of manure through insect composting as well as investigating co-digestion opportunities to enhance biogas production would support famers currently using these systems. Full article
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27 pages, 4264 KB  
Article
A Fast Integral Terminal Sliding Mode Buck Converter with a Fixed-Time Observer for Solar-Powered Livestock Smart Collars
by Shiming Zhang, Haochen Ouyang, Shengqiang Shi, Guichang Fang, Zhen Wang, Xinnan Du and Boyan Huang
Agriculture 2026, 16(7), 746; https://doi.org/10.3390/agriculture16070746 - 27 Mar 2026
Abstract
Fully maintenance-free smart collars for range cattle, sheep and deer must survive years of uncontrolled grazing under highly variable shade and motion conditions. This paper presents an ultra-low-power buck converter governed by a fast integral terminal sliding mode controller (FITSMC) with a fixed-time [...] Read more.
Fully maintenance-free smart collars for range cattle, sheep and deer must survive years of uncontrolled grazing under highly variable shade and motion conditions. This paper presents an ultra-low-power buck converter governed by a fast integral terminal sliding mode controller (FITSMC) with a fixed-time observer. A new reaching law retains the initial sliding manifold and a negative-power term maintains the constant switching gain to preserve robustness near the surface while attenuating chattering without widening the bandwidth. The fixed-time observer estimates the irradiance and load changes and provides a feed-forward correction, tightening the output regulation regardless of initial conditions. Load step tests with moderate resistance swings showed the proposed method recovers noticeably faster and exhibits slightly lower overshoot than a recent method based on a two-phase power reaching law, while visible inductor current spikes are also suppressed. Simulations under daily grazing profiles confirmed tight output regulation adequate for microwatt data logging and periodic long-range (LoRa) bursts. The sleep mode quiescent current remained in the 9 microamps range, eliminating the need for manual recharge across multi-season field deployments. By integrating robust power electronics with collar-grade solar harvesting, the circuit offers a truly maintenance-free energy path for untethered livestock wearables and supports sustainable precision agriculture. Full article
(This article belongs to the Section Artificial Intelligence and Digital Agriculture)
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30 pages, 2146 KB  
Article
Research on a Precision Counting Method and Web Deployment for Natural-Form Bothriochloa ischaemum Spikes and Seeds Based on Object Detection
by Huamin Zhao, Yongzhuo Zhang, Yabo Zheng, Erkang Zeng, Linjun Jiang, Weiqi Yan, Fangshan Xia and Defang Xu
Agronomy 2026, 16(7), 706; https://doi.org/10.3390/agronomy16070706 - 27 Mar 2026
Abstract
Bothriochloa ischaemum is a key forage species with strong grazing tolerance and high nutritional value, making precise quantification of spike and seed traits essential for germplasm evaluation and yield prediction. However, the compact architecture and minute seed size in natural field conditions render [...] Read more.
Bothriochloa ischaemum is a key forage species with strong grazing tolerance and high nutritional value, making precise quantification of spike and seed traits essential for germplasm evaluation and yield prediction. However, the compact architecture and minute seed size in natural field conditions render manual counting inefficient and labor-intensive. To address this limitation, this study presents a non-destructive and automated quantification framework integrating advanced object detection and regression analysis for accurate in situ estimation of spikes and seed numbers. To further address the challenges of dense spike detection caused by occlusion and small object sizes, this study developed a modified model named YOLOv12-DAN by integrating DySample dynamic upsampling, ASFF feature fusion, and NWD loss, which achieved a mean average precision (mAP) of 91.6%. Meanwhile, for the detection of dense kernels on compact spikes, an improved YOLOv12 architecture incorporating an Explicit Visual Center (EVC) module was proposed to enhance multi-scale feature representation. The optimized model attained a bounding box precision of 96.5%, a recall rate of 86.4%, an mAP50 of 94.3%, and an mAP50-95 of 73.9%. Furthermore, a univariate linear regression model based on 132 spike samples verified the reliable consistency between the predicted and actual seed counts, with a mean absolute error (MAE) of 6.30, a mean absolute percentage error (MAPE) of 9.35, and an R-squared (R2) value of 0.808. Finally, the model was deployed through a lightweight end-to-end web application, enabling real-time field operation and promoting its applicability in breeding programs and agronomic decision-making. This study provides a robust technical pathway for automated phenotyping and precision forage improvement. Full article
(This article belongs to the Special Issue Digital Twins in Precision Agriculture)
14 pages, 2087 KB  
Article
On-Farm Nitrification Inhibitor Application to Urine Patches in Reducing Nitrous Oxide Emissions
by Surinder Saggar, Thilak Palmada, Peter Berben and Liyin Liang
Agronomy 2026, 16(7), 701; https://doi.org/10.3390/agronomy16070701 - 26 Mar 2026
Abstract
In livestock-grazed pastures, urine patches are a major contributor of nitrous oxide (N2O) emissions, and the use of nitrification inhibitors (NIs) has the potential to reduce N losses from urine patches using New Zealand (NZ)-devised Spikey®—a ground-based machine that [...] Read more.
In livestock-grazed pastures, urine patches are a major contributor of nitrous oxide (N2O) emissions, and the use of nitrification inhibitors (NIs) has the potential to reduce N losses from urine patches using New Zealand (NZ)-devised Spikey®—a ground-based machine that measures the change in soil conductivity from the deposited urine patches. Our ongoing research suggests that the efficacy of on-farm targeted NIs treatment requires suitable inhibitor concentrations within urine patches to be achieved to reduce N2O emissions. This study evaluates the effect of varying NI rates and volumes on reducing N2O emissions. The application rates for NIs were 1.6 g and 3.2 g dicyanamide (DCD) patch-1 and 0.96 g and 1.92 g of 3, 4-dimethylpyrazole phosphate (DMPP) patch−1, using 100, 150, and 200 mL inhibitor solutions. These rates were higher than those used in previous studies to ensure an adequate supply of inhibitors above the threshold concentration within the urine patch and to enhance the inhibitor efficacy in reducing N2O emissions. This study points to two important aspects: Determine the optimum inhibitor concentration required to eliminate, minimise/reduce N2O emissions and ensure that at the optimised amounts of inhibitor application rates, inhibitor residues are below their maximum residue level (MRL) in the food chain and environment, and eliminate their potential harm to human health. Full article
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10 pages, 229 KB  
Article
Irrigated Winter Malting Barley Cultivar Performance in Cold Desert and Cold Semiarid Environments
by Leonard M. Lauriault, Kevin Lombard, Gasper K. Martinez and Murali K. Darapuneni
Agronomy 2026, 16(7), 695; https://doi.org/10.3390/agronomy16070695 - 26 Mar 2026
Abstract
Growers in the grain-producing continental cold desert and cold semiarid regions are interested in the local adaptation of winter malting barley (Hordeum vulgare) as a potential alternative crop to winter wheat (Triticum aestivum). Variety selection for specific environments is [...] Read more.
Growers in the grain-producing continental cold desert and cold semiarid regions are interested in the local adaptation of winter malting barley (Hordeum vulgare) as a potential alternative crop to winter wheat (Triticum aestivum). Variety selection for specific environments is a critical first step in producing high yields of winter malting barley at the same production costs. Twenty-two winter malting barley entries were planted under irrigation in randomized complete blocks at New Mexico State University’s Agricultural Science Center at Farmington (cold desert; 3 replicates) and Rex E. Kirksey Agricultural Science Center at Tucumcari (cold semiarid; 4 replicates) in September 2023 and harvested for grain in July 2024. All entries at Tucumcari were heavily grazed by wildlife over winter, which may have influenced grain production of some varieties, although there was no site × cultivar interaction for grain yield, which ranged from 2558 to 4157 kg ha−1. Irrigation and N fertilization differences between sites likely influenced (p < 0.0001) grain yield and grain protein (4421 and 2172 kg grain yield ha−1 at Farmington and Tucumcari, respectively; 109 and 93 g grain protein kg−1 at Farmington and Tucumcari, respectively). Future research in cold desert and semiarid regions should evaluate cultivar differences regarding irrigation and nutrient management. Full article
(This article belongs to the Section Water Use and Irrigation)
27 pages, 8176 KB  
Article
Climate and Vegetation Dominate Lake Eutrophication in the Inner Mongolia–Xinjiang Plateau (2000–2024)
by Yuzheng Zhang, Feifei Cao, Yuping Rong, Linglong Wen, Wei Su, Jianjun Wu, Yaling Yin, Zhilin Zi, Shasha Liu and Leizhen Liu
Remote Sens. 2026, 18(7), 988; https://doi.org/10.3390/rs18070988 - 25 Mar 2026
Abstract
Lakes on the Inner Mongolia–Xinjiang Plateau (IMXP) are increasingly vulnerable to eutrophication under climate change and human pressure, yet long-term monitoring remains limited by sparse field sampling. Here, we reconstruct multi-decadal trophic dynamics across the IMXP using Landsat time series and temporally transferable [...] Read more.
Lakes on the Inner Mongolia–Xinjiang Plateau (IMXP) are increasingly vulnerable to eutrophication under climate change and human pressure, yet long-term monitoring remains limited by sparse field sampling. Here, we reconstruct multi-decadal trophic dynamics across the IMXP using Landsat time series and temporally transferable machine-learning models and further quantify the underlying natural and anthropogenic drivers. We compiled monthly in situ water-quality observations (chlorophyll-a, Chl-a; total phosphorus, TP; total nitrogen, TN; Secchi depth, SD; and permanganate index, CODMn;) and calculated the trophic level index (TLI). After rigorous quality control and monthly aggregation, we compiled a dataset of 1345 matched lake–month samples spanning 2000–2024, and divided it into a training set (n = 1076; ≤2019) and an independent test set (n = 269; 2020–2024) to evaluate temporal transferability. We utilized Google Earth Engine to generate monthly surface reflectance composites from Landsat 7 ETM+, Landsat 8 OLI, and Landsat 9 OLI-2. Four supervised regression algorithms—ridge regression (RR), support vector regression (SVR), random forest (RF), and eXtreme Gradient Boosting (XGBoost)—were trained to estimate TLI. On the independent test period, XGBoost performed best (R2 = 0.780, RMSE = 3.290, MAE = 1.779), followed by RF (R2 = 0.770, RMSE = 3.364), SVR (R2 = 0.700, RMSE = 3.842), and RR (R2 = 0.630, RMSE = 4.267); we then used XGBoost to reconstruct monthly and yearly TLI for 610 perennial grassland lakes from 2000 to 2024. From 2000 to 2024, the annual mean TLI (48–49) across the IMXP exhibited a statistically significant upward trend (slope = 0.0158 TLI yr−1; 95% confidence interval (CI) = 0.0050–0.0267; p = 0.006). Meanwhile, spatial heterogeneity was distinct (TLI: 41.51–59.70). High values concentrated in endorheic and desert–oasis basins (e.g., Eastern Inner Mongolia Plateau, >51), whereas lower values characterized high-altitude regions (e.g., Yarkant River, <45). Overall, trends ranged from −0.49 to 0.51 yr−1, increasing in 54% of lakes (15.6% significantly) and decreasing in 46% (15.4% significantly). Attribution analyses identified NDVI (33.92%) and temperature (21.67%) as dominant drivers (55.59% combined), followed by precipitation (13.99%) and human proxies (30.42% combined: population 10.66%, grazing 10.31%, built-up 9.45%). Across 53 sub-basins, NDVI was the primary driver in 28, followed by temperature (11), population (7), precipitation (3), grazing (3), and built-up land (1); notably, the top two drivers explained 56.6–87.1% of variations. TWFE estimates revealed bidirectional NDVI effects (significant in 31/53): positive associations in 22 basins were linked to nutrient retention, contrasting with negative effects in nine basins associated with agricultural return flows. Temperature effects were significant in 15 basins and predominantly negative (14/15), except for the Qiangtang Plateau. Overall, eutrophication risk across the IMXP lake region reflects the combined influences of climatic conditions, vegetation conditions, and human activities, with their relative contributions varying among basins. Full article
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26 pages, 12260 KB  
Article
Quantitative Analysis of Wind Erosion Drivers Using Explainable Artificial Intelligence: A Case Study from Inner Mongolia, China
by Yong Mei, Batunacun, Chang An, Yaxin Wang, Yunfeng Hu, Yin Shan and Chunxing Hai
Land 2026, 15(4), 531; https://doi.org/10.3390/land15040531 - 25 Mar 2026
Abstract
Wind erosion is a multidimensional, dynamic process driven by natural and anthropogenic factors, but existing statistical methods struggle to capture its complex nonlinear relationships, resulting in incomplete quantification of drivers and their spatial variability. To address this, we integrate the Revised Wind Erosion [...] Read more.
Wind erosion is a multidimensional, dynamic process driven by natural and anthropogenic factors, but existing statistical methods struggle to capture its complex nonlinear relationships, resulting in incomplete quantification of drivers and their spatial variability. To address this, we integrate the Revised Wind Erosion Equation (RWEQ)model with explainable artificial intelligence to disentangle the spatiotemporal positive and negative effects of dominant drivers and their synergistic interactions in Inner Mongolia. Results show that, from 2000–2022, wind erosion has been decreasing on average by 1.1 t·ha−1·yr−1, mainly in the western deserts and locally in Hulunbuir sandy land. Severe erosion is mostly due to nature (78.7%) rather than anthropogenic (21.3%). Normalized difference vegetation index (NDVI), clay content (CL), windy days (WD), precipitation (PRE), temperature (TEM), and sand content (SA) were found to be the most important drivers of wind erosion. Critical threshold conditions for severe wind erosion are NDVI < 0.14, CL < 12%, GD > 26, PRE < 73.15 mm, and SA > 66%. When there is a certain combination of variables, wind erosion risk is greatly increased, which mainly happens in the western part of Alxa, Bayannur, and the area near the desert edge. Wind erosion control should shift toward region-specific precision management, including engineering protection, optimized grazing management, and vegetation restoration. Full article
(This article belongs to the Section Land Innovations – Data and Machine Learning)
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1 pages, 148 KB  
Correction
Correction: Vaca-Cárdenas et al. Floristic Composition of Andean Moorlands and Its Influence on Natural Pasture Productivity: Implications for the Sustainable Management of Alpaca Grazing in Guamote, Ecuador. Conservation 2026, 6, 15
by Maritza Lucia Vaca-Cárdenas, Julio Mauricio Oleas-Lopez, Santiago Fahureguy Jiménez-Yánez, Freddy Renan Costales Zavala, Pedro Vicente Vaca-Cárdenas, Diego Francisco Cushquicullma-Colcha and Marcelo Eduardo Moscoso-Gómez
Conservation 2026, 6(2), 39; https://doi.org/10.3390/conservation6020039 - 25 Mar 2026
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Abstract
The authors would like to make the following correction to the published paper [...] Full article
13 pages, 670 KB  
Article
Productive and Physiological Response of Grazing Steers Supplemented with Energy–Protein Supplements During Summer in a Subtropical Humid Region
by Martina Verdaguer and Pablo Rovira
Sustainability 2026, 18(7), 3189; https://doi.org/10.3390/su18073189 - 24 Mar 2026
Viewed by 73
Abstract
In the context of climate change and increased frequency of droughts, summer supplementation of grazing cattle may improve productivity and resilience of pastoral systems. However, the provision of supplements may increase the risk of heat stress in cattle. This study aimed to evaluate [...] Read more.
In the context of climate change and increased frequency of droughts, summer supplementation of grazing cattle may improve productivity and resilience of pastoral systems. However, the provision of supplements may increase the risk of heat stress in cattle. This study aimed to evaluate the productive and physiological response of grazing steers supplemented during summer. Three independent studies were conducted over three summers (2020–2023). In each experiment, steers grazing native grasslands with access to shade were allotted to one of two treatments: control (CONT) and supplementation (SUPPL), in a completely randomized block design. SUPPL steers were group-fed in the morning three days per week with an energy–protein ration at a level of 1.98% body weight (BW) on days of feeding. Pasture attributes, animal performance, respiration rate (RR), and body temperature (BT) were analyzed using a mixed model. According to the temperature–humidity index, cattle were exposed to heat stress 32% of the time. Summer supplementation increased average daily gain and final body weight of steers. Although supplementation temporarily increased BT (morning) and RR (afternoon), daily average RR and BT were similar for both treatments. These findings show that summer supplementation improves animal performance of grazing steers without increased risk of severe heat stress. These results are aligned with the concept of sustainable livestock intensification, which aims to enhance animal source foods to feed a growing population without causing collateral animal welfare issues. Full article
(This article belongs to the Special Issue Sustainable Animal Production and Livestock Practices)
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17 pages, 7796 KB  
Essay
Study on Differences in Lipid Composition of Camel Milk with Different Forage-to-Concentrate Diets
by Haiyun Yang, Sanshan Sun, Yusong Shen, Zhiwei Li, Changjiang Zang, Yong Chen and Fengming Li
Animals 2026, 16(7), 1002; https://doi.org/10.3390/ani16071002 - 24 Mar 2026
Viewed by 100
Abstract
This study was conducted to investigate the effect of different concentrate-to-roughage ratios on the lipid composition of camel milk. Thirty-six Qiangar Bactrian camels were randomly allocated to three groups: the control group (C, grazing plus roughage only), the low-concentrate group (L, grazing plus [...] Read more.
This study was conducted to investigate the effect of different concentrate-to-roughage ratios on the lipid composition of camel milk. Thirty-six Qiangar Bactrian camels were randomly allocated to three groups: the control group (C, grazing plus roughage only), the low-concentrate group (L, grazing plus roughage with 2 kg/d concentrate supplementation), and the high-concentrate group (H, grazing plus roughage with 4 kg/d concentrate supplementation). The trial comprised an 18-day adaptation period followed by a 42-day feeding period (60 days total). A systematic lipidomics approach revealed significant differences in milk lipid profiles among the three dietary treatments. High-concentrate diets markedly remodeled the milk lipidome (especially in positive-ion mode mass spectrometry), with the most pronounced differences detected between the C and H groups. Multivariate analyses (PCA and PLS-DA) confirmed that varying concentrate levels significantly affected milk lipid composition, and the separation between H and C was greater than that between L and C. KEGG annotation and enrichment analysis indicated that lipid-related pathways, notably glycerophospholipid metabolism, were significantly affected by dietary concentrate levels. These findings provide theoretical support for optimizing camel feeding strategies to improve milk nutritional quality and offer scientific guidance for camel husbandry and dairy product development. Full article
(This article belongs to the Section Animal Products)
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26 pages, 3374 KB  
Article
Sloping Terrain May Increase Grazing Pressure on Rangelands: Evidence from Herbivore Jaw Activity and Locomotion
by Eugene David Ungar, Maya Zahavi, Hillary Voet, Shilo Navon, Aharon Bellalu and Tal Svoray
Environments 2026, 13(3), 177; https://doi.org/10.3390/environments13030177 - 23 Mar 2026
Viewed by 183
Abstract
A deeper understanding of the relationships between the local and landscape scales in herbivore foraging should place the management of rangeland production systems on a firmer footing. The objective was to test whether local-scale landscape features modulate the coupling between locomotion and eating, [...] Read more.
A deeper understanding of the relationships between the local and landscape scales in herbivore foraging should place the management of rangeland production systems on a firmer footing. The objective was to test whether local-scale landscape features modulate the coupling between locomotion and eating, thereby altering the pattern of landscape-scale grazing pressure. We studied shepherded small-ruminant herds on hilly semiarid rangeland by integrating acoustic monitoring to detect jaw movements, GPS to track location and movement, and GIS to link location to landscape attributes. Based on 69 one-day foraging routes, minutely rate of jaw movement (RJM) as a function of time-into-foraging-route showed a unimodal concave shape but did not respond to path angle. Minutely movement velocity responded convexly to time-into-foraging-route, and the quadratic term for path angle was negative and highly significant. The response to path angle was concave and symmetrical for uphill and downhill travel. Based on the empirical evidence that increasing path angle reduces velocity but not RJM and a set of reasonable associated assumptions, it is inferred that more jaw movements are performed per unit area scanned by the animal. It is further inferred abductively that more bites are removed per unit area and that more mass is removed per unit area, and hence, grazing pressure is more intense on sloping terrain than on level areas. For a given duration of foraging route, an increase in density of bite placement at the local behavioral scale implies a contraction in the surface area of the daily herd footprint at the landscape scale. This has implications for how carrying capacity of such areas should be defined. Full article
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13 pages, 752 KB  
Article
Anthrax in Albania: A Comprehensive Analysis of Epidemiology, Laboratory Diagnosis, and National Control Strategies in Animals
by Xhelil Koleci, Erson Dhimospira, Sulejman Kullolli, Mandy Elschner, Heinrich Neubauer and Gamal Wareth
Vet. Sci. 2026, 13(3), 300; https://doi.org/10.3390/vetsci13030300 - 22 Mar 2026
Viewed by 119
Abstract
Anthrax is one of the most significant zoonotic diseases in Albania due to its endemic presence in livestock, the potential for occupational exposure, and human cases. Although the implementation of risk-based livestock immunization, animal movement restrictions, and appropriate carcass disposal, the efficacy of [...] Read more.
Anthrax is one of the most significant zoonotic diseases in Albania due to its endemic presence in livestock, the potential for occupational exposure, and human cases. Although the implementation of risk-based livestock immunization, animal movement restrictions, and appropriate carcass disposal, the efficacy of targeted management remains limited in certain outbreaks due to insufficient enforcement of these measures. Their efficacy is specifically diminished by insufficient disinfection, the absence of grazing bans in contaminated pastures, and the absence of designated burial sites for the safe disposal of dead animals. District-level data on animal anthrax control programs were collected and analyzed for the period 2021–2025. In addition, a retrospective analysis of national datasets covering the same period was conducted using data from the national surveillance system, alongside a review of the relevant scientific and grey literature and aggregated program and routine surveillance data. Analysis showed that anthrax affected 149 animals in 97 farms, and the average number of animals per infected farm declined from 1.70 to 1.08, indicating a slight reduction within-farm outbreak. Hotspots for human anthrax were aligned with the animal cases and persisted particularly in the southern districts. The peak of outbreaks was in 2023, primarily driven by cattle (n = 32) and sheep (n = 24). Equine cases appeared only in 2024, with small clusters of 3 cases in both 2024 and 2025. Caprine cases remained consistently low throughout the period. Nevertheless, the number of outbreaks and within-herd cases are decreasing due to more rapid identification and response. Targeted surveillance on animal outbreaks provides critical insights into disease spread and links among affected farms in Albania. Therefore, One Health genomic surveillance and antibiotic susceptibility testing of Bacillus anthracis isolates are essential for understanding its epidemiology, transmission routes, and for tracing the sources of infection across humans, animals, and the environment. Full article
(This article belongs to the Section Veterinary Microbiology, Parasitology and Immunology)
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16 pages, 1340 KB  
Article
Effect of Grazing Intensity and Frequency on Forage Accumulation and Agronomic Characteristics of Tropical Mixed Pastures
by Bruna Zanini Uzan, Luciana Gerdes, Waldssimiler Teixeira de Mattos, Taise Robinson Kunrath, Stela Soares Zamboin, Cristina Maria Pacheco Barbosa, Gabriela Aferri and Flavia Maria de Andrade Gimenes
Grasses 2026, 5(1), 15; https://doi.org/10.3390/grasses5010015 - 20 Mar 2026
Viewed by 105
Abstract
This study evaluated combinations of defoliation frequencies and intensities to identify grazing strategies that optimize forage accumulation and morphological composition in mixed pastures of Marandu palisadegrass (Urochloa brizantha cv. Marandu) with the legume Macrotyloma axillare. Treatments consisted of pre-grazing heights of [...] Read more.
This study evaluated combinations of defoliation frequencies and intensities to identify grazing strategies that optimize forage accumulation and morphological composition in mixed pastures of Marandu palisadegrass (Urochloa brizantha cv. Marandu) with the legume Macrotyloma axillare. Treatments consisted of pre-grazing heights of 30 and 40 cm (defining defoliation frequency) combined with post-grazing heights of 15 and 20 cm (defoliation intensity), in a 2 × 2 factorial randomized block design with four repetitions. Forage accumulation rate, morphological component mass, and leaf area index (LAI) were evaluated under rotational stocking. The highest forage accumulation rates of grass and its stems occurred at a pre-grazing height of 30 cm. A taller pre-grazing height (40 cm) resulted in greater pre-grazing forage mass, leaf and stem mass of Marandu palisadegrass and LAI, but it also increased the amount of dead material and post-grazing stem mass. The greatest Macrotyloma forage accumulation occurred under grazing strategies of 30–20 cm and 40–15 cm. Lenient defoliation (20 cm post-grazing height) favored post-grazing leaf mass, whereas severe defoliation (15 cm) favored stem mass. Marandu palisadegrass showed higher LAI at 40 cm pre-grazing height (4.7) than at 30 cm (3.6), with slightly greater values under 20 cm (4.3) than 15 cm (4.1) post-grazing height, while Macrotyloma axillare exhibited low LAI. Across all grazing strategies, the legume mass decreased over time. Therefore, future studies should explore alternative grazing strategies and periodic reseeding of Macrotyloma axillare to maintain its presence in mixed tropical pastures. Full article
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27 pages, 1297 KB  
Review
The Trophic Cascade Effects of Marine Mesozooplankton: Theory, Dynamics, and Responses to Global Change
by Mianrun Chen
Microorganisms 2026, 14(3), 697; https://doi.org/10.3390/microorganisms14030697 - 19 Mar 2026
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Abstract
Marine mesozooplankton (0.2–20 mm), as a critical trophic link between primary producers and higher trophic levels, are pivotal drivers of trophic cascades regulating pelagic ecosystem structure and function. This review synthesizes recent advances in understanding mesozooplankton-mediated trophic cascades (MMTC), with a focus on [...] Read more.
Marine mesozooplankton (0.2–20 mm), as a critical trophic link between primary producers and higher trophic levels, are pivotal drivers of trophic cascades regulating pelagic ecosystem structure and function. This review synthesizes recent advances in understanding mesozooplankton-mediated trophic cascades (MMTC), with a focus on selective feeding mechanisms, and presents an original, integrated quantitative framework that fills gaps in quantification and prediction of MMTC. This framework includes the following: a dual-pathway conceptual model distinguishing density-mediated and trait-mediated cascades; a three-level grazing rate correction model addressing long-standing underestimations of mesozooplankton direct grazing rate on phytoplankton; a comprehensive Cascade Strength Index for quantifying cascade intensity; an extended numerical model—NPMZ model (Nutrient–Phytoplankton–Microzooplankton–Mesozooplankton) for simulating MMTC dynamics and their biogeochemical impacts. The review further elucidates the spatiotemporal heterogeneity of MMTC and its implications for plankton community size structure and biological carbon pump efficiency. It also systematically assess the combined impacts of global change drivers (ocean warming, acidification, eutrophication) on MMTC and their ecological consequences. This review advances the theoretical framework of marine trophic cascade research by establishing a unified quantitative paradigm for MMTC and provides mechanistic insights and predictive tools for understanding how climate change modulates pelagic food web dynamics and marine ecosystem services. Moreover, the proposed integrated research paradigm combining molecular tools, multi-factor experiments, and high-resolution numerical modeling offers a critical roadmap for future MMTC research in the Anthropocene. This provides a scientific basis for the conservation and adaptive management of marine ecosystems under global change. Full article
(This article belongs to the Special Issue Microbial Food Webs)
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20 pages, 1665 KB  
Review
Production, Transport, and Metabolism of Volatile Fatty Acids in the Yak Rumen: Unraveling the Unique Mechanisms Underpinning High-Altitude Adaptation
by Zhenyu Zhu, Jianbo Zhang, Ali Mujtaba Shah, Qunying Zhang, Binqiang Bai and Lizhuang Hao
Microorganisms 2026, 14(3), 696; https://doi.org/10.3390/microorganisms14030696 - 19 Mar 2026
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Abstract
Volatile fatty acids (VFAs), the primary end-products of microbial fermentation in the ruminant forestomach, supply approximately 70% of the host’s energy requirements and play a pivotal role in maintaining energy homeostasis. While the mechanisms governing ruminal VFA production, absorption, and metabolism are well-characterized [...] Read more.
Volatile fatty acids (VFAs), the primary end-products of microbial fermentation in the ruminant forestomach, supply approximately 70% of the host’s energy requirements and play a pivotal role in maintaining energy homeostasis. While the mechanisms governing ruminal VFA production, absorption, and metabolism are well-characterized in common ruminants like dairy and beef cattle, a systematic integration of these processes in yaks, an iconic species long-adapted to the extreme Qinghai–Tibet Plateau, remains incomplete. This review synthesizes current knowledge on the entire VFA pathway in the yak rumen, from production to tissue metabolism. We detail the critical roles of functional microbes, including fibrolytic bacteria and Prevotella, in VFA synthesis and how their activity is dynamically regulated by dietary composition and seasonal shifts. Building on the unique structural features of the yak rumen epithelium, the review analyzes VFA absorption mechanisms involving both passive diffusion and carrier-mediated transport. Furthermore, we systematically outline the metabolic fates and energy partitioning strategies of VFAs across the rumen epithelium, liver, and peripheral tissues. This synthesis aims to elucidate the highly efficient and adaptive physiological basis of VFA metabolism that underpins the yak’s exceptional ability to utilize energy under the low-energy conditions of the high-altitude environment. Ultimately, this work seeks to provide a theoretical foundation for understanding plateau-adapted energy efficiency and to inform precision nutritional strategies for ruminants in alpine regions. Full article
(This article belongs to the Section Veterinary Microbiology)
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