Search Results (34,681)

Background: Tumor neoantigens are key targets for personalized vaccines and T-cell therapies, yet most pipelines focus on neoantigens derived from SNV/small indel and often yield a limited number of high-quality candidates. SVs are prevalent in tumors and can generate novel chimeric sequences and neopeptides, making them a promising additional source of neoantigens. However, SV-derived neoantigen prediction remains challenging due to breakpoint uncertainty, isoform-dependent coding inference, and limited integration of multi-dimensional evidence and reproducibility. Methods: We developed SVNeoPP (Structural Variant Neoantigen Prediction and Prioritization), an end-to-end workflow for SV-derived neoantigen analysis. SVNeoPP takes WGS and RNA-seq as inputs, performs SV calling and annotation, and reconstructs altered transcripts and coding sequences in a traceable, isoform-aware manner to generate candidate peptides. Candidates are prescreened by integrating antigen-processing features with HLA binding prediction, and then hierarchically filtered and prioritized based on transcript expression, LC–MS/MS proteomics evidence, immunogenicity predictions, and sequence similarity to experimentally validated neoantigen databases. SVNeoPP is implemented in Snakemake to enable modular extension, checkpoint-based restarts, and end-to-end reproducibility. Results: Using a hepatocellular carcinoma (HCC) multi-omics dataset as a proof of concept, we demonstrated the performance of SVNeoPP and obtained a high-priority shortlist of candidate peptides. Compared with other methods, SVNeoPP substantially expanded the candidate search space for SV-derived neoantigens and showed more favorable distributions of antigen-processing and HLA binding features. Conclusions: SVNeoPP provides a reusable, traceable, and interpretable multi-dimensional evidence-driven framework for SV-derived neoantigens. As a complementary module to SNV/small-indel pipelines, it broadens the neoantigen candidate repertoire and generates ranked candidates with interpretable evidence to facilitate downstream prioritization and decision-making. Full article

Coffee, as a perennial commodity crop, plays a crucial role in global agricultural markets, regional livelihoods, and poverty alleviation. Yunnan Province of China (21°8′–29°15′N) represents the northernmost coffee-growing region worldwide, and its production has gained increasing attention in international markets. However, the absence of a spatially explicit and high-resolution coffee distribution dataset has constrained environmental assessment, land-use analysis, and policy-making in this subtropical and marginal growing region. In this study, we developed the first 10 m resolution Arabica coffee distribution dataset for Yunnan Province for the year 2023 using Sentinel-2 optical imagery and Shuttle Radar Topographic Mission (SRTM) terrain data within the Google Earth Engine (GEE) platform. An object-based workflow was implemented to generate spatially coherent mapping units, followed by supervised classification to identify coffee plantations. The resulting map achieved an overall accuracy (OA) of 0.87, with user accuracy (UA), producer accuracy (PA), and F1 score of 0.90, 0.96, and 0.93 for the coffee class, demonstrating its reliability for regional-scale applications. Feature contribution analysis indicates that shortwave infrared (SWIR) and red-edge information, particularly during the dry season, plays an important role in coffee discrimination. These results enhance confidence in the ecological relevance and stability of the mapping framework. The proposed workflow provides a practical and transferable approach for perennial crop mapping in complex mountainous environments. More importantly, the generated high-resolution coffee distribution dataset establishes a spatial baseline for monitoring land-use dynamics, assessing ecological impacts, and supporting sustainable coffee development in southwestern China. Full article

Against the backdrop of ongoing climate change, the Qinghai–Tibet Plateau, a region highly sensitive to climatic variation, exhibits intricate spatiotemporal patterns in reference crop evapotranspiration (ET_O), with significant implications for regional water-resource planning. This study selected four agro-climatic zones across the plateau region (TSA, TSH, TAZ, and WCH). Long-term daily observations from 28 meteorological stations were used to estimate ET_O via the FAO 56 Penman–Monteith equation. This extensive dataset enabled robust trend analysis using the Mann–Kendall test, alongside a cloud-model framework, and analyses of sensitivity and contributions to evaluate ET_O’s spatiotemporal evolution, its distributional uncertainty, and the underlying drivers. Results reveal pronounced regional heterogeneity in the interannual variability of ET_O. Annual ET_O declined in TSH and TSA (trend rates of −1.12 and −6.58 mm·10a⁻¹, respectively) and increased in TAZ and WCH (15.76 and 10.74 mm·10a⁻¹, respectively). At monthly and seasonal timescales, ET_O exhibited an unimodal pattern, with the greatest stability in winter and spring and lower stability in summer and autumn. The cloud-model parameter He indicates that ET_O stability is greatest in TSH and weakest in WCH, with He values of 7.15 and 12.29 mm, respectively. Contribution-rate analyses identify T_max and T_mean as the principal determinants of rising ET_O across all study zones, reflecting the largest individual contributions. Temperature-related factors together account for the majority of ET_O variability across the regions, with their absolute contributions ranging from 5.61% to 8.63%, well above those of aerodynamic factors (0.62–1.78%). Stability assessments indicate that ET_O is generally more unstable than its meteorological drivers, with substantial regional disparities, implying that ET_O evolution cannot be explained by a single controlling factor. Overall, the study characterizes the uncertainty in ET_O variations under complex terrain, highlights the value of the cloud model for refined hydrological assessments, and provides a scientific basis for adaptive agricultural water-resource management in the region. Full article

Large-scale forest ecological restoration is commonly expected to improve habitat quality and promote population growth of forest-dependent herbivores. Yet, whether vegetation recovery facilitates or constrains herbivore growth and habitat use at local scales within nature reserves remains unclear, as vegetation recovery and canopy closure might alter forage availability and lead to ecological mismatch between vegetation features and population dynamic. Here, we used the endangered species South China sika deer as the study species, and its dominant distribution region—Qingliangfeng Biosphere Reserve—as the study area. Using decadal camera-trapping data (2015–2024) and extracted vegetation and other environmental variables, we quantified decadal trends in sika deer activity intensity and interannual variation in vegetation (leaf area index, LAI, and normalized difference vegetation index, NDVI). We incorporated topographic and anthropogenic disturbance variables and applied generalized linear mixed models and generalized linear models to analyze its habitat use. We found that: (1) Numbers of independent photographs and the relative abundance index of sika deer increased significantly and consistently from 2015 to 2024. (2) LAI exhibited substantial interannual variability without a stable trend. In contrast, segmented regression identified a clear temporal breakpoint in NDVI, with a significant increasing trend before 2021 followed by a pronounced decline thereafter. (3) In all years, distance to settlement had a significant and negative effect on activity intensity, whereas distance to road, elevation, and year had significant positive effects. LAI and NDVI showed negative and weak effects on sika deer activity intensity. In specific years, LAI had a significantly negative effect in early periods whereas NDVI became significantly negative in mid and late periods. Other environmental variables exhibited interannual heterogeneity. Our findings demonstrate that vegetation recovery within the reserve does not automatically improve habitats for forest-dependent herbivores and could lead to a potential ecological mismatch. Full article

With increasing demand for wellness tourism, identifying the key factors influencing emotional perceptions is essential for optimizing destination planning and management. Although Anhui Province has experienced rapid growth in wellness tourism destinations in recent years, scientific understanding of tourists’ emotional perceptions and their driving mechanisms has lagged behind this rapid expansion, a gap that can be addressed by integrating big data with spatial analysis to provide a scientific perspective for optimizing destination planning and informing regional wellness tourism policy. To address this gap, this study conducts a sentiment analysis of wellness bases in Anhui Province using user-generated content (UGC) data. Sentiment scores were quantified via SnowNLP, while kernel density, time-series, and multivariate statistical analyses were applied to examine spatial distributions, temporal dynamics of sentiments and review volumes, and emotional driving factors. The results indicate a spatial pattern of higher density in the south, lower density in the north, and dual-core agglomeration, closely linked to natural resource endowments. Temporally, sentiment scores rise in spring and summer and decline in winter, while review volumes peak in spring and autumn. Overall regression analyses reveal a significant positive effect of green coverage and a negative effect of accommodation prices. In the typological analysis, sentiment scores of Forest Wellness Bases (FWBs) relate to green coverage and negative ions, while Hydrological Wellness Bases (HWBs), Traditional Chinese Medicine Wellness Bases (TCMWBs), and Wellness Towns (WTs) are driven by the combined effects of facility services, locational price, and ecological environment. These findings provide a scientific basis for the sustainable development and differentiated management of wellness tourism destinations. Full article

Diffusion models have emerged as a powerful class of generative models, demonstrating impressive results across visual domains such as image and video synthesis. This survey provides a comprehensive taxonomy of generative models, with a particular focus on diffusion models and their applications in enhancing visual fidelity for text-to-image and text-to-video generation. We discuss the theoretical foundations of diffusion models, including their formulation through stochastic differential equations, and analyze the forward noising and reverse denoising processes that enable stable training and high-quality generation. The survey further categorizes diffusion architectures, including pixel-space and latent-space models, and examines their design choices, training strategies, and trade-offs across different resolution regimes. In addition, we review noise characteristics in real-world imaging domains and discuss their implications for diffusion-based models. Denoising strategies are analyzed by distinguishing between in-model denoising mechanisms and external denoising techniques used in preprocessing and post-processing pipelines. The survey also summarizes commonly used datasets and evaluation metrics for generative modeling, providing a practical perspective on benchmarking and model comparison. Finally, we discuss current challenges, including computational efficiency, scalability, and robustness to diverse noise distributions, and outline potential directions for future research. This survey aims to provide a structured reference for understanding diffusion models and their applications in visual generation tasks. Full article

The concentration of heavy metals in the environment has been steadily increasing, raising concerns about their adverse effects on ecosystems and human health. Fine-grained particulate matter is of particular concern due to its enhanced mobility, bioavailability, and potential for inhalation exposure. Facilities involved in the mechanical processing of electronic waste (e-waste) represent a significant potential source of metal-containing fine particles. In this study, crushed e-waste components containing precious metals were separated into particle-size fractions ranging from 3.0 to 0.15 mm using a vibratory sieving system. The elemental composition of the individual fractions was determined by energy-dispersive X-ray fluorescence spectrometry (ED-XRF), while the spatial distribution of selected metals in fine fractions was further investigated using scanning electron microscopy combined with energy-dispersive X-ray spectroscopy (SEM–EDS). The results demonstrate that e-waste contains a wide range of heavy non-ferrous metals whose distribution is strongly dependent on particle size. A pronounced enrichment of metals was observed in the finest fractions, particularly below 0.25 mm. Compared to the coarse fraction (>3 mm), the zinc concentration increased by approximately one order of magnitude, while chromium, nickel, and cadmium exhibited increases of up to approximately 20-fold. Lead showed particularly high enrichment, reaching approximately 2 wt.% in the finest fraction (<0.15 mm), corresponding to nearly fiftyfold enrichment relative to the coarse fraction. Tin concentrations also increased markedly, in some cases by up to two orders of magnitude. Trace amounts of arsenic and selenium were detected in the finest fractions, whereas mercury was not detected. The combined ED-XRF and SEM–EDS results confirm that fine-grained e-waste fractions are the dominant carriers of hazardous metals and respirable particles generated during mechanical processing. These findings highlight the dual character of fine fractions as both a critical environmental and occupational risk and a potentially valuable secondary resource. The study emphasizes the importance of controlled handling, effective dust management, and targeted processing strategies to minimize human exposure while enabling efficient recovery of valuable metals from e-waste. Full article

Multi-Key Fully Homomorphic Encryption (MK-FHE) is essential for secure multi-party computation but currently faces significant scalability bottlenecks due to linear computational growth and low bootstrapping throughput. To address these limitations, we propose DMBB-RCB, a novel fully homomorphic, bit-wise Dynamic Multi-Key Block-Binary Ring-Compact Bootstrapping scheme. Our contribution is threefold. First, we integrate the Block Binary Distribution into the dynamic setting, reducing the complexity of the core blind rotation operation from O(P⋅n) to O(p⋅k) (where k ≪ n) by leveraging key sparsity. Second, we implement an amortized ring packing strategy that aggregates multiple Learning with Errors (LWE) ciphertexts into the coefficients of a single Ring Learning with Errors (RLWE) polynomial, enabling the parallel refreshing of messages. Third, we introduce a Ring-Compact extraction architecture that natively translates RLWE states into Multi-Key Regev–Gentry–Sahai–Waters (RGSW) ciphertexts via scheme switching. Unlike traditional pipelines that suffer from severe network latency due to interactive multi-party key-switching after each bootstrapping, our architecture keeps the data entirely within the ring domain. This completely eliminates intermediate interaction rounds, enabling depth-unbounded homomorphic evaluations with zero interaction between participants during the computation phase (interaction is strictly reserved for the final joint decryption step). The proposed scheme supports the dynamic addition of participants without parameter re-generation. Theoretical analysis confirms that DMBB-RCB significantly reduces latency and enhances throughput compared to existing dynamic MKHE solutions. Full article

The design day flight schedule (DDFS) plays a pivotal role in airport simulation and infrastructure planning. Despite its importance, previous studies and global guidelines offer only broad recommendations for DDFS preparation, lacking detailed methodologies and empirical validation. This study proposes a systematic, data-driven approach for generating a future DDFS that accounts for projected demand, airline behavior, and regional traffic characteristics. Leveraging historical flight operation data and probabilistic distributions, the proposed method captures existing patterns and anticipated market changes comprehensively. To realistically define each flight’s operational characteristics, a structured 10-step procedure is employed to generate and assign attributes—such as aircraft type, origin/destination airport, and turnaround time—based on empirical patterns and logical constraints. The proposed approach is applied to Incheon International Airport as a case study, demonstrating its practical utility and scalability. The generated DDFSs are shown to be consistent with target-year forecasts in terms of peak-hour operations and fleet composition, with deviations remaining within a small error range. Additional validation confirms that key operational characteristics, including airline shares, connection patterns, and turnaround times, are reproduced with acceptable accuracy. By bridging the gap between high-level guidance and implementable practice, this study contributes a replicable framework for future DDFS generation and provides actionable insights for airport planners aiming to better anticipate operational demands. Full article

Accurate and stable acquisition of the spatial distribution of soybean planting areas is essential for supporting precision agricultural monitoring and ensuring food security. However, crop remote-sensing mapping for specific regions still faces critical data bottlenecks: high-precision, large-scale pixel-level annotation is costly, resulting in scarce available labeled samples that make it difficult to construct large-scale training datasets. Although parameter-intensive models such as FCN and SegNet can achieve sufficient end-to-end training on large-scale public remote sensing datasets like LoveDA, when directly applied to the data-limited dataset in this study area, the models are prone to overfitting, leading to a significant decline in generalization ability. To address these issues, this study proposes a lightweight U-shaped semantic segmentation model, SimSDRU-Net. The model utilizes a pre-trained VGG-16 backbone to extract shallow texture and deep semantic features. The pre-trained weights mitigate the impact of overfitting in data-limited settings. In the decoding stage, a parameter-free lightweight SimAM attention module enhances effective soybean features and suppresses soil background redundancy, while an embedded S-DRU unit fuses multi-scale features for deep complementary reconstruction to improve edge detail capture. A label dataset was constructed using Sentinel-2 images as the data source and Menard County (USA) as the study area. The USDA CDL was used as a foundation for the dataset, with Google high-resolution images serving as visual interpretation aids. In the context of the experiment, Deeplabv3+ and U-Net++ were compared with U-Net under identical conditions. The results demonstrated that SimSDRU-Net exhibited optimal performance, with MIoU of 89.03%, MPA of 93.81%, and OA of 95.96%. Specifically, SimSDRU-Net uses the SimAM attention module to generate spatial attention weights by analyzing feature statistical differences through an energy function, so as to adaptively enhance soybean texture features. Meanwhile, the S-DRU unit groups, dynamically weights, and cross-branch reconstructs multi-scale convolutional features to preserve fine boundary details and achieve accurate segmentation of soybean plots. The present study demonstrates that SimSDRU-Net integrates lightweight design and high precision in data-limited scenarios, thereby providing effective technical support for the rapid extraction of soybean planting areas in North America. Full article

Addressing the thermal management challenges of prismatic aluminum shell lithium battery modules in electric vehicles under high-rate charge–discharge conditions, this study proposes a multi-objective optimization design method for integrated biomimetic liquid cooling plates. By integrating various highly efficient heat transfer structures from nature, including fractal-tree-like networks, leaf vein branching systems, and spider web radial distribution, a novel biomimetic liquid cooling plate topology was constructed. A multi-physics coupled numerical model considering electrochemical heat generation, thermal conduction, convective heat transfer, and thermal stress deformation was established. The NSGA-II algorithm was employed to globally optimize 12 design variables including channel geometric parameters, operating conditions, and structural dimensions, achieving collaborative optimization objectives of maximum temperature minimization, temperature uniformity maximization, pressure drop minimization, and structural lightweighting. The weight coefficients for the four optimization objectives were determined through the Analytic Hierarchy Process (AHP) with verified consistency (CR = 0.02 < 0.10), ensuring rational priority allocation aligned with automotive safety standards. The optimization results demonstrated that compared to the initial design, the optimal solution reduced the maximum temperature under 3C discharge conditions by 9.9% to 34.7 °C, decreased the temperature difference by 31.3% to 3.3 °C, lowered the pressure drop by 24.6% to 2150 Pa, reduced structural mass by 4.0%, and decreased maximum stress by 16.7%. Quantitative comparison with single biomimetic structures under identical boundary conditions showed that the integrated design achieved a 3.3% lower maximum temperature and 25.7% better flow uniformity than the best-performing single structure, demonstrating the synergistic advantages of multi-biomimetic integration. These synergistic performance improvements can be attributed to the hierarchical multi-scale architecture where fractal networks provide macro-scale flow distribution, leaf vein branches ensure meso-scale coverage, and spider web radials achieve micro-scale thermal matching. Long-term cycling tests conducted at 1C/1C rate with 25 ± 1 °C ambient temperature showed that the optimized design maintained a capacity retention rate of 92.3% after 1000 charge–discharge cycles, demonstrating excellent durability. The complex biomimetic channel structure can be fabricated using selective laser melting technology with minimum feature sizes below 0.3 mm, indicating promising manufacturing feasibility. The research findings provide theoretical guidance and technical support for the engineering design of high-performance battery thermal management systems. Full article

Background/Objectives: Federated learning has emerged as a promising paradigm for enabling collaborative artificial intelligence in healthcare while preserving data privacy. However, most existing frameworks focus on isolated tasks and lack integrated pipelines that combine classification, segmentation, explainability, and automated clinical reporting. Methods: This study proposes FedIHRAS, a privacy-preserving federated learning framework designed for multi-institutional radiological analysis. The system integrates multi-task deep learning modules, including pathology classification using a modified ResNet-50 backbone, anatomical segmentation, explainability through Grad-CAM, and automated report generation supported by semantic aggregation using SNOMED CT. The framework employs confidence-weighted aggregation, differential privacy mechanisms, and secure aggregation protocols to ensure privacy and robustness across heterogeneous institutional datasets. Results: Experimental evaluation was conducted across four large-scale chest X-ray datasets representing simulated institutional nodes, totaling approximately 874,000 images. FedIHRAS achieved high diagnostic performance with strong cross-institutional generalization and demonstrated improved robustness under non-IID data distributions. Additional experiments showed favorable communication efficiency, effective privacy–utility trade-offs, and strong agreement with expert radiologist assessments. Conclusion: The proposed FedIHRAS framework demonstrates that federated learning can support scalable, privacy-preserving, and clinically meaningful radiological AI systems. By integrating multi-task learning, explainability, and automated reporting within a unified federated architecture, the framework addresses key limitations of existing approaches and contributes to the development of collaborative AI in healthcare. Full article

Infrared UAV small target detection is critical in areas such as military reconnaissance, disaster monitoring, and border patrol. However, it faces challenges due to the small size of targets, weak texture, and complex backgrounds in infrared images. Existing deep learning-based object detection models often learn spurious correlations between targets and their backgrounds. This leads to poor generalization and higher rates of false positives and missed detections in complex scenes. To overcome feature bias and improve performance, this paper proposes an enhanced detection framework based on causal reasoning. The framework builds on the advanced CSFPR-RTDETR detector. Guided by the principles of structural causal models, it explicitly separates causal and non-causal features in the feature space. Feature debiasing is achieved through a three-path approach. First, a causal data augmentation module is introduced. It applies frequency perturbations drawn from a Gaussian distribution to non-causal features. This strengthens the model’s robustness against mixed disturbances. Second, a counterfactual reasoning module is integrated into the backbone network. This module generates counterfactual samples to intervene in the feature distribution, helping the model identify and utilize causal features more effectively. Third, a causal attention mechanism module is added to the encoder. By distinguishing and weighting causal and non-causal features, it guides the model to focus on features that are essential for detecting targets. Experiments on the HIT-UAV public dataset show that the proposed framework improves mAP@50 by 5.6% and mAP@50:95 by 1.8%. Visualization analysis further confirms that the framework enhances feature discrimination and overall detection performance. Full article

Generative adversarial networks (GANs) are increasingly applied to mitigate extreme class imbalance in intrusion detection systems, yet reported improvements often obscure role augmentation intensity and adversarial stability. This paper presents a controlled ablation study that isolates the impact of adversarial objective choice, augmentation ratio, and training duration on GAN-based minority data augmentation for highly imbalanced tabular cybersecurity data. Using the UWF-ZeekData22 dataset, nine MITRE ATT&CK tactic-versus-benign classification tasks are evaluated under augmentation ratios of 0.25 and 0.50 and training durations of 400 and 800 epochs. Four GAN variants—Vanilla GAN, Conditional GAN (cGAN), WGAN, and WGAN-GP—are assessed using stratified cross-validation and five classical classifiers representing diverse inductive biases. The results reveal consistent structural patterns. Moderate augmentation (r = 0.25) with controlled training (400 epochs) yields the most stable and reliable improvement in minority recall. Wasserstein-based objectives demonstrate superior stability under aggressive augmentation and prolonged training, while conditional GANs frequently exhibit recall collapse in ultra-sparse regimes. Increasing augmentation volume does not uniformly improve performance and may introduce distributional overlaps that degrade linear and margin-based classifiers. Tree-based classifiers remain largely invariant once sufficient minority density is achieved. These findings demonstrate that adversarial calibration is more important than architectural complexity for improving the detection of rare attacks. The study provides practical guidance for designing robust GAN-based augmentation pipelines under extreme cybersecurity class imbalance. Full article

This paper investigates the stability of telehandlers operating on inclined terrain through a sequential methodological approach. In a first stage, stability is assessed using quasi-static methods based on force and moment equilibrium, including the load transfer matrix and the stability pyramid. These approaches account for gravitational and inertial effects through equivalent external forces and moments applied at the global centre of gravity, enabling efficient evaluation of load redistribution and proximity to rollover thresholds under generalized quasi-static conditions. The application of these methods highlights intrinsic limitations when addressing structurally complex machines such as telehandlers equipped with a pivoting rear axle and evolving mass distribution due to boom motion. In particular, quasi-static approaches require a priori assumptions regarding the effective rollover axis and cannot fully capture the coupled geometric and contact interactions between rear axle articulation limits, centre of gravity migration, tyre–ground interface behaviour, and support polygon evolution. To overcome these limitations, a nonlinear dynamic multibody model based on the three-dimensional Bond Graph (3D Bond Graph) methodology is introduced. The model is implemented within a virtual tilt–rotation test platform and validated against experimental results obtained from ISO 22915-14 stability tests. The comparison confirms compliance with normative requirements and demonstrates that the dynamic framework captures condition-dependent rollover mechanisms and transitions between distinct virtual rollover axes that cannot be fully explained by quasi-static formulations. Unlike most previous studies, which focus on fixed configurations or forward-driving scenarios, the proposed framework analyzes stability evolution under spatial inclination while accounting for structural articulation constraints. The explicit identification of rollover axis transitions induced by rear axle articulation provides a deeper mechanistic interpretation of telehandler stability and supports the use of high-fidelity dynamic simulation as a complementary tool for test interpretation, experimental planning, and the development of predictive stability and operator assistance systems. Full article