Search Results (1,197)

Sidr (Ziziphus spina-christi L.) is an underutilized fruit native to arid and semi-arid regions that possesses considerable nutritional and phytochemical value. However, its potential for controlled lactic fermentation and development into value-added fermented products has received limited scientific attention. This study investigated the effects of five brine formulations on the controlled fermentation of Sidr fruit pickles and monitored changes in physicochemical properties, bioactive compounds, microbial dynamics, texture, color, and sensory attributes during 90 days of storage at ambient temperature. The treatments consisted of 10% NaCl (control), NaCl supplemented with sodium sorbate, NaCl with sucrose and vinegar, NaCl with sucrose and Lactobacillus plantarum starter culture, and NaCl with sucrose, vinegar, and garlic. Brine formulation significantly influenced fermentation kinetics, microbial succession, and product quality throughout storage. The inoculated treatment containing L. plantarum exhibited the most rapid acidification, reaching a pH of 4.02 and titratable acidity of 0.24%, while maintaining the highest lactic acid bacteria population (>9 log CFU g⁻¹) and enhanced microbiological stability. This treatment also showed superior retention of ascorbic acid, total phenolic compounds, antioxidant activity, and texture compared with the non-inoculated treatments. Pearson correlation analysis and principal component analysis (PCA) further demonstrated strong associations between starter-culture fermentation, bioactive compound preservation, and overall product quality. Sensory evaluation indicated that all treatments remained acceptable throughout storage; however, the inoculated samples consistently received the highest scores for taste, texture, and overall acceptability. Overall, the results indicate that controlled lactic fermentation using L. plantarum represents an effective approach for enhancing the quality, stability, and bioactive retention of fermented Sidr fruit products, supporting the valorization of this underexploited fruit resource for sustainable food applications. Full article

Hawthorn (Crataegus pinnatifida) pulp is characterized by high levels of organic acids and polyphenols, yet its strong acidity limits sensory acceptance. In this study, Streptococcus thermophilus 540, Lactobacillus brevis 647, and their mixed culture (1:1) were applied to ferment hawthorn pulp. Changes in organic acids, individual phenolics, free amino acids, volatile compounds, antioxidant capacity, and global metabolic profiles were systematically investigated using targeted analysis combined with untargeted metabolomics. Fermentation markedly reshaped the metabolic composition of hawthorn pulp, with mixed fermentation exhibiting the most pronounced metabolic reprogramming. Co-fermentation significantly enhanced phenolic derivatives and aromatic amino acid-related metabolites, while promoting a more balanced distribution of organic acids and esters, contributing to improved antioxidant potential and flavor coordination. Multivariate analyses confirmed that mixed fermentation formed a distinct metabolic pattern rather than a simple additive effect of single strains, characterized by expanded metabolic diversity and pathway complementarity. These findings provide mechanistic insight into strain-dependent and synergistic metabolic modulation during fruit fermentation and support mixed lactic acid bacteria fermentation as a promising strategy for improving the functional and sensory quality of hawthorn-based products. Full article

Paprika is a spice obtained from the dehydration and grinding of red pepper fruits, primarily from the Capsicum annuum species. Its etymology comes from Slavic Balkanian languages and was adopted in Hungarian. The crop originated in America, where it was domesticated by pre-Columbian civilizations over 6000 years ago (specifically in present-day Mexico) for medicinal and culinary purposes. Following the Spanish arrival in the Americas in the 15th century, pepper was introduced first in Spain (Sevilla, Extremadura and Murcia) and later in the rest of the Old World. The agroclimatic conditions of different Mediterranean regions made it an essential crop, turning these regions into centers of production and giving this spice a sense of cultural identity. The purpose of this study lies in the technological and nutritional significance of paprika in the modern food industry, where it is demanded as a natural colorant, preservative and source of bioactive compounds, such as antioxidants and carotenoids. Despite its prevalence, the existing literature is often fragmented into specific disciplines. This article distinguishes itself by proposing a holistic approach expanding the study from its historical evolution to its socioeconomic impact, including its agronomic characteristics and industrial-scale production. It is recommended that the research community and producers focus on the sustainability of processing methods while preserving cultural authenticity, ensuring the preservation of the functional and culinary relevance of this spice. Full article

The Knyszyn Forest is one of the major lowland forest complexes of north-eastern Poland, yet its myxobiota remains insufficiently documented. We surveyed Eumycetozoa in three forest nature reserves—Krzemianka, Jałówka, and Las Cieliczański—to assess local species richness, substrate occurrence, and new distributional records. Field surveys were combined with moist-chamber cultures, and species were identified from mature sporocarps using macro- and microscopic morphology. The inventory yielded 761 occurrence records representing 80 identified taxa. Moist-chamber cultures produced more records and species than field collection, while field surveys detected taxa fruiting naturally during the study period; together, both methods produced a more complete inventory than either method alone. Three species, Paradiacheopsis solitaria, Macbrideola rutilipedata, and Licea operculata, were recorded for the first time in Poland; these national novelties were included among the 14 taxa recorded for the first time in north-eastern Poland. Most records came from dead wood and bark, although other dead organic substrates also contributed to the recorded substrate spectrum. The resulting dataset provides a regional baseline for future biodiversity surveys in the Knyszyn Forest. Full article

Fruit rot disease is a major obstacle for starapple production in Vietnam. Therefore, the aims of the current study were (i) to identify the causing agents for fruit rot in starapple, (ii) to select isolates of Trichoderma spp. as antagonists, and (iii) to determine efficient approaches to control the disease. The pathogens were isolated from symptomatic starapple fruits collected in Can Tho City, Vietnam. The antagonists were isolated from the soils of healthy starapple farms. The three pathogenic isolates with the fastest growth (20.5–45.0 mm) and the largest infection spot (14.5–16.5 mm) were Calonectria candelabra C-SA01, C. candelabra C-SA06, and C. sulawesiensis C-SA07. Four Trichoderma isolates, T-SA01, T-SA06, T-SA10, and T-SA14, were selected because they consistently showed high antagonistic efficiency against C-SA01, C-SA06, and C-SA07 in dual-culture assays at 56.7–69.5%, 50.4–70.8%, and 62.6–69.2%, respectively. ITS-based results tentatively assigned T-SA01 to T. harzianum and T-SA06, T-SA10, and T-SA14 to T. asperellum. Under in vivo conditions, each Trichoderma sp. isolate was combined with different spraying methods. The result revealed that the four antagonists showed identical outcomes in reducing fruit rot disease. Spraying Trichoderma spp. both one day before and after inoculation resulted in the best biocontrol, with the highest disease-reducing rate at 44.2% at day 5. These newly found antagonists should be further tested in actual starapple farms to reveal their true potency. Full article

Rapid demographic change across countries, with people from diverse ethnic backgrounds and preferences, mandates new nectarine (Prunus persica var. nucipersica) cultivars with varying skin and flesh colors and times of availability. After a 4-year initial screening with 21 cultivars, long-term phenological characteristics, yield, and fruit quality attributes of several cultivars of yellow- and white-fleshed nectarine, harvested at two intervals (Harvest 1 and Harvest 2) and stored for two storage durations (Period 1 and Period 2), were studied over four years. ‘Royal Bright’ consistently exhibited the latest bloom (higher Julian day) and, together with ‘Giant Pearl’, required greater cumulative growing degree days (GDD) than other cultivars to reach both Harvest 1 and Harvest 2 each year. Fruit GDD differences at Harvest 2 as compared to Harvest 1 in ‘Candy Pearl’ were the longest, and those of ‘Giant Pearl’, ‘BN7’, and ‘Royal Bright’ were shorter among all cultivars. ‘Burnectseven’ (‘BN7’), ‘Flame’, and ‘Royal Bright’ always had higher yield, while all “Pearl” series (‘Giant Pearl’, ‘Majestic Pearl’, ‘Candy Pearl’) were in the low-yielding cultivars. ‘Candy Pearl’, ‘Majestic Pearl’, and ‘BN7’ nectarines often had larger fruit than other cultivars. Fruit picked at the second harvest had lower firmness than that picked at the first harvest in all cultivars every year. Fruit of ‘Candy Pearl’ often had higher firmness, but those of ‘BN7’ and ‘Royal Bright’ had lower firmness, at the times of both Harvest 1 and Harvest 2. Fruits of ‘BN7’ and ‘Candy Pearl’ often had higher soluble solids concentrations at Harvest 1 and Harvest 2, and after keeping the fruit in storage for Period 1 and Period 2. According to this study, ‘Candy Pearl’ is recommended as a good choice for the early market, as the fruit in this cultivar was mild with high flavor and attractive red skin and white flesh. Also, ‘Majestic Pearl’, ‘BN7’, and ‘Flame’ can be grown for the mid-to-late August market. ‘Majestic Pearl’ and ‘Flame’ had large fruit with a moderate level of russet and a split pit in some years, and thus, any cultural practices that may contribute to fruit russet and split pit should be avoided. Details on recommendations for suitable cultivars, harvest stages, and storage durations are provided in this study. Full article

This research presents bacterial cellulose (BC) filaments knitted from agro-industrial by-products. The fermentation media came from pressed fruit (beetroot, ginger, grape), vegetable beverages, bagasse with different concentrations (1/2.5, 1/5, 1/7.5, and 1/10), and a control medium with unrefined sugar from sugarcane or panela. The BC filaments were obtained from a mixed culture of bacteria and yeast (SCOBY); functionalized using NaOH purification treatment and glycerol plasticizer; untwisted and twisted with 1-, 2-, and 4-ply; and characterized physically and mechanically by weight, diameter, tensile strength, Young’s modulus, and elongation. The untwisted and 2-ply twisted BC filaments from the fruit medium 1/2.5 showed tensile strength of 272 MPa and 155 MPa, respectively. Finally, control panela filaments with 1-, 2-, and 4-ply and fruit filaments with 2- and 4-ply were knitted in wet states. This research demonstrates the use of by-products to produce BC filaments with knitting properties for textile applications. Full article

To evaluate the effects of saline water on the yield and quality of tomatoes, a late fruit-setting stage irrigation experiment was conducted in a greenhouse using two cultivars: medium-fruited, tasty Strawberry tomato (fresh-eating) and large-fruited Maofen tomato (fresh/processing). For this, plants were grown in pots containing substrate, and five irrigation water electrical conductivity (EC) levels (1.0 as control, 2.6, 4.2, 5.8, and 7.4 dS m⁻¹) were applied for each cultivar, resulting in a 2 × 5 factorial design with 10 treatments in total. Then, tomato growth, fruit ion composition, and quality attributes were evaluated. The results showed that 1.0–7.4 dS m⁻¹ saline water had no significant impact on the plant height, stem diameter, single-fruit weight, or total yield of either cultivar. However, Strawberry tomato’s marketable yield decreased by 23.5% at 7.4 dS m⁻¹. The yield per plant of Maofen tomato was 2.7 times that of Strawberry tomato. Fruit Na⁺ content increased with EC for both cultivars; Maofen tomato had higher Na⁺ and a lower K⁺/Na⁺ ratio, with greater ion content responses to salinity. Regression analysis revealed distinctly nonlinear responses in key yield, ion, and quality parameters across the salinity gradient. The fruit comprehensive quality score (CQS) rose with EC, and Strawberry tomato’s average CQS increase (109%) was significantly higher than Maofen tomato’s. In conclusion, saline irrigation initiated when the fourth-cluster fruits attained 60% of the final harvested diameter, at EC ≤ 5.8 dS m⁻¹ for Strawberry tomato and ≤7.4 dS m⁻¹ for Maofen tomato, improved fruit quality without compromising yield. Strawberry tomato is recommended for quality-oriented production, whereas Maofen tomato is better suited for yield-oriented production, providing scientific support for saline water utilization in greenhouse soil-less cultivation. Full article

Healthcare-associated infections (HAIs) remain a major public health concern, contributing to increased morbidity, mortality, and antimicrobial resistance. Healthcare workers (HCWs) are recognized as key vehicles in the transmission of nosocomial pathogens, primarily via contaminated hands and medical devices. This study assessed the effectiveness of hand hygiene protocols among HCWs, their correlation with bloodstream infections, and the potential of natural antimicrobial agents as complementary preventive measures. Between January and June 2025, 128 hand samples were collected from HCWs in surgical, intensive care, and internal medicine units of hospitals managed by ASL Caserta (Marcianise, n = 65; Piedimonte Matese, n = 30; Sessa Aurunca, n = 18; Maddaloni, n = 15). Sampling was performed upon entry to clinical areas and after antiseptic handwashing, using Rodac TSA plates to quantify microbial load (CFU/cm²). Isolates were identified via MALDI-TOF, and multidrug resistance was confirmed using the Phoenix BD system. Microbial growth was detected in 54.7% of samples. Coagulase-negative staphylococci, mainly Staphylococcus epidermidis and S. hominis, accounted for 67.1% of positive cultures, followed by Enterobacteriaceae (28.6%). Comparison with concurrently collected blood cultures revealed potential overlapping pathogens, with Staphylococcus spp. prevalence ranging from 35 to 56% and Gram-negatives from 18 to 39. Selected isolates were further tested for susceptibility to natural antimicrobial agents, derived from hop, red vine leaf, green tea, and pomegranate fruit, as well as thyme essential oil. Thyme essential oil (Thymus vulgaris) demonstrated notable antimicrobial activity, in several cases surpassing that of standard hygiene agents. These findings highlight not only that maintaining high standards of hand hygiene, proper care of invasive devices, and continuous microbiological surveillance is critical for preventing HAIs, but also that incorporating natural antimicrobial compounds into hygiene protocols may provide an effective and sustainable adjunct to reduce microbial contamination and combat infections caused by multidrug-resistant organisms. Full article

Despite a large number of African-born individuals residing in the United States, there is a significant disparity in how this community accesses and utilizes mental health treatment. Low screening rates for common mental health concerns is one crucial part of ongoing inequities in mental healthcare access. Willingness to engage in screening is negatively impacted by a lack of culturally responsive ways to make screening more acceptable and stigma with mental health. This study therefore aimed to examine the perceived acceptability and utility of community-developed patient vignettes created to increase willingness to be screened for common mental health concerns. Employing a qualitative approach, a community advisory board (CAB) (n = 5) was enlisted to co-develop vignettes outlining an African community member’s symptoms of anxiety and subsequent help-seeking behavior. Two focus groups of community members (n = 18) provided qualitative feedback on the vignettes and shared their general attitudes towards mental health and recommendations for mental health screening and treatment in the African community. Using a hybrid inductive and deductive qualitative descriptive approach and classifying responses based on the socioecological model, four major themes emerged from the data: (1) between support and strain: the role of family; (2) reducing stigma: community voices as education; (3) culture as a barrier and a bridge; and (4) the importance of stories that reflect lived experience. Overall, participants were receptive to the culturally-responsive mental health vignettes and provided fruitful suggestions for how these stories can be used to reduce stigma and increase willingness to seek screening and treatment in African-born residents of the United States. Full article

In citrus production, there is an absence of established standards of critical Nickel (Ni) content for deficiency, sufficiency, and excess, which could be used to determine the nutritional status of plant Ni. In this study, to explore the critical Ni concentrations for deficiency and excess, we conducted a hydroponic pot culture experiment and investigated the effects of Ni levels on flower and fruit development, dry weight, and nutrient accumulation of Newhall navel orange. We found that 0.8 and 6.4 mg L⁻¹ of solution Ni were the turning point concentrations of Ni deficiency and excess for plants, respectively. Solution Ni deficiency (0 to 0.8 mg L⁻¹ of Ni) tended to promote vegetative growth and increase the dry weight of new leaves, but suppress flower bud number and fruit development. It also significantly promoted the accumulation of N, P, K, Ca, and Mg in old leaves and N and K in roots, but significantly reduced that of Fe, Mn, and Zn in roots. Excess solution Ni (6.4 to 12.8 mg L⁻¹ of Ni) reduced the water content of fruit peel and was accompanied by fruit cracking during the fruit expansion period, inhibited new leaf growth and whole plant biomass or dry weight, and significantly decreased nutrient accumulation in roots. Equations of dry weight and solution Ni levels for each plant organ were established, showing that 3.93 to 4.72 mg L⁻¹ of Ni was the sufficient concentration of solution Ni for the growth and development of Newhall navel orange, with the corresponding range of Ni contents in new and old leaves being 17.87 to 20.42 and 10.24 to 11.64 mg kg⁻¹, respectively. These findings provide reference for the recommended range of Ni sufficient for citrus growth. Full article

Pear fruits host diverse microbial communities that influence postharvest quality, spontaneous fermentation, and susceptibility to microbial contamination. This study characterizes the fungal communities associated with naturally fallen overripe pears (Pyrus communis) using ITS2 amplicon sequencing combined with culture-dependent approaches. The fungal community exhibited low diversity and was dominated by Ascomycota (99%), primarily Saccharomycetes (91.8%), with Hanseniaspora, Aureobasidium, and Microcyclospora representing more than 90% of the total microbial community. Culture-dependent isolation confirmed Hanseniaspora uvarum as the dominant yeast species (~89%), followed by Metschnikowia spp. and Pichia spp. Pairwise co-culture assays, quantified using the Relative Interaction Index, demonstrated predominantly competitive interactions, with fast-growing H. uvarum exerting suppressive effects on slower-growing species. Among the isolated yeasts, Metschnikowia fructicola exhibited antibacterial activity against all tested bacteria Staphylococcus aureus, Listeria innocua and Salmonella typhimurium. The strongest antibacterial activity was exerted against the foodborne pathogen S. aureus. In a pear juice model system, co-cultivation with M. fructicola resulted in the elimination of S. aureus within four days, while yeast viability was maintained. These findings observe the fermentative yeasts distributed in overripe pears and demonstrate the potential of M. fructicola to inhibit bacterial growth under controlled conditions. The results provide a preliminary basis for further studies on fungal succession, yeast interactions, and the biocontrol potential of pear-associated yeasts. For broader ecological conclusions, larger-scale studies across locations, seasons, cultivars, and decay stages are required. Full article

Ancient olives are considered a major resource of gene pool, adapted across ages to ever changing environments. The ancient ‘Mehras’ olive is the only cultivar inscribed on the UNESCO Representative List of the Intangible Cultural Heritage Of Humanity as recognized for its longevity and historical significance. However, detailed genetic and phenotypic analyses are still missing. Nineteen ‘Mehras’ accessions were collected from northern Jordan and subjected to inter-simple sequence repeat (ISSR) marker analysis and to a set of phenotypic parameters (leaf, fruit, and stone). ISSR analysis revealed similarity values ranging between 0.53 and 1.00, indicating moderate to high genetic diversity. Bayesian clustering and dendrogram analyses identified two major genetic clusters with limited admixture, indicating extended clonal propagation in addition to the transport of planting material. Phenotypic parameters revealed tangible variation among accessions, with major influence of fruit and stone traits followed by leaf traits. Strong correlations were observed between key traits, including fruit width and flesh thickness, while an inverse relationship was observed between flesh and stone percentages. Multivariate analysis further revealed clear separation among investigated accessions. The Mantel test showed a moderate correlation between genetic and phenotypic distances. Spatial analysis suggested weak geographic structuring of diversity, indicating exchange of plant material. ‘Mehras’ olive harbors structured genetic and phenotypic diversity influenced mainly by adaptation and traditional cultivation practices. These findings provide a foundation for conservation, breeding, and sustainable utilization of this ancient and culturally significant cultivar. Full article

Fermentation is one of the oldest food preservation and transformation strategies developed by human societies. Its cultural appropriation predates even the scientific understanding of the role of microorganisms and their metabolic functions. However, significant knowledge gaps remain regarding the mechanisms underlying these effects, as well as safety considerations and regulatory frameworks. This review addresses these gaps through an integrative analysis of the literature, including peer-reviewed studies and systematic reviews focused on food microbiology, microbial dynamics, and health-promoting mechanisms. In particular, yogurt and cheese are addressed as case studies to review the past and present of these ancestral foods in terms of the evolution of fermentation processes, from spontaneous fermentation to controlled and standardized systems, along with the modern biotechnological tools used to characterize and monitor complex microbial communities. Substrates such as stevia, fruits, vegetables, and plant-based milk substitutes are also explored. The scope encompasses enzymatic transformation of raw substrates, biosynthesis of bioactive metabolites, effects on the gut microbiota, and the use of fermented foods as vehicles for “biotic” compounds. Particular attention is paid to safety and regulatory aspects. Fermented foods are culturally important and promising functional platforms, although standardized regulatory frameworks and stronger mechanistic and clinical evidence regarding their health benefits are still needed. Full article

Croatia’s diverse agroecological zones, from Mediterranean coastal areas to continental lowlands, enable the cultivation of a broad portfolio of traditional fruit species that contribute simultaneously to biodiversity conservation, rural livelihoods, and the development of value-added food and beverage products. This review compiles and harmonizes evidence on six economically and culturally relevant crops and product chains—grapevine and wine, apple, pear, quince, sour cherry, mulberry, and plum with the traditional spirit šljivovica—focusing on genetic resources and cultivar diversity, agronomic and environmental performance, bioactive composition and potential health relevance, processing routes and by-product valorization, and the socio-economic roles of geographical indications, gastronomy, and tourism. Across species, the literature highlights recurring sustainability levers: safeguarding indigenous and old cultivars as reservoirs of adaptive traits under climate change; reducing chemical inputs through cultivar choice, organic and low-input systems, cover crops, and resistant genotypes; strengthening circularity by converting pomace and other residues into spirits, vinegars, functional ingredients, feed, compost, or energy carriers; and increasing rural value capture through branding, protected origin schemes, and experiential tourism. At the same time, production systems face shared constraints, including fragmentation of holdings, labour shortages, phytosanitary pressures, and the need to optimize processing technologies to preserve sensory and bioactive quality while meeting safety and regulatory requirements. By integrating crop-specific evidence with cross-cutting sustainability themes, this review outlines a coherent framework for positioning traditional Croatian fruit resources and their derived products within contemporary sustainable food system transitions. Full article