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16 pages, 4784 KiB  
Article
In Vitro and In Vivo Testing of Decellularized Lung and Pancreas Matrices as Potential Islet Platforms
by Alexandra Bogomolova, Polina Ermakova, Arseniy Potapov, Artem Mozherov, Julia Tselousova, Ekaterina Vasilchikova, Alexandra Kashina and Elena Zagaynova
Int. J. Mol. Sci. 2025, 26(14), 6692; https://doi.org/10.3390/ijms26146692 - 12 Jul 2025
Viewed by 264
Abstract
The treatment of type 1 diabetes through pancreatic islet transplantation faces significant limitations, including donor organ shortages and poor islet survival due to post-transplantation loss of extracellular matrix support and inadequate vascularization. Developing biocompatible scaffolds that mimic the native islet microenvironment could substantially [...] Read more.
The treatment of type 1 diabetes through pancreatic islet transplantation faces significant limitations, including donor organ shortages and poor islet survival due to post-transplantation loss of extracellular matrix support and inadequate vascularization. Developing biocompatible scaffolds that mimic the native islet microenvironment could substantially improve transplantation outcomes. This study aimed to create and evaluate decellularized (DCL) matrices from porcine organs as potential platforms for islet transplantation. Porcine lung and pancreatic tissues were decellularized using four different protocols combining detergents (Triton X-100, SDS and SDC) with optimized incubation times. The resulting matrices were characterized through DNA quantification and histological staining (H&E and Van Gieson). Islet viability was assessed in vitro using Live/Dead staining after 3 and 7 days of culture on the matrices. In vivo biocompatibility was evaluated by implanting matrices into rat omentum or peritoneum, with histological analysis at 1-, 4-, and 8 weeks post-transplantation. Protocols 3 (for lung tissue) and 4 (for pancreas tissue) demonstrated optimal decellularization efficiency with residual DNA levels below 8%, while preserving the collagen and elastin networks. In vitro, islets cultured on decellularized lung matrix had maintained 95% viability by day 7, significantly higher than the controls (60%) and pancreatic matrix (83%). The omentum showed superior performance as an implantation site, exhibiting minimal inflammation and fibrosis compared to the peritoneum sites throughout the 8-week study period. These findings establish DCL as a promising scaffold for islet transplantation due to its superior preservation of ECM components and excellent support of islet viability. This work provides a significant step toward developing effective tissue-engineered therapies for diabetes treatment. Full article
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15 pages, 553 KiB  
Article
Diabetologists’ Knowledge and Prescription of Physical Activity in Southeast Europe
by Krešimir Martinac, Spomenka Ljubić, Dario Rahelić, Tomas Matić, Tomislav Perković and Slavica Sović
Medicina 2025, 61(7), 1244; https://doi.org/10.3390/medicina61071244 - 10 Jul 2025
Viewed by 249
Abstract
Background and Objectives: Physical activity represents a cornerstone in the management of diabetes mellitus; however, the majority of persons with diabetes remain insufficiently active. Current guidelines emphasise the need to educate individuals with diabetes on physical activity at each clinical visit. The [...] Read more.
Background and Objectives: Physical activity represents a cornerstone in the management of diabetes mellitus; however, the majority of persons with diabetes remain insufficiently active. Current guidelines emphasise the need to educate individuals with diabetes on physical activity at each clinical visit. The aim of the study was to develop an adequate instrument and test diabetologists’ knowledge on physical activity and related guidelines in diabetes, as well as investigate their prescribing habits. Materials and Methods: The instrument was developed with four diabetologists using Delphi and RAND appropriateness methods, then distributed to diabetologist societies in 12 Southeast European countries for anonymous completion by their members. Data were analysed using Mann–Whitney U test and Pearson  x2 test. Results: Out of 302 analysed respondents, 123 (41%) worked at university hospitals, at average four days a week in outpatient departments, mean number of patients was 16 a day; 95 (32%) had a PhD, and 105 (35%) university teaching positions. Median overall knowledge was 15 (Q1–Q3, 13–17) out of 22 maximum points. 26% diabetologists knew recommended level of physical activity for people with diabetes. Physical activity goals were discussed during check-ups in 53.0% of cases; re-education and goal adjustments were provided in 33.4% when health status changed; and clear instructions were documented in 25.5% of medical records. Conclusions: Diabetologists’ overall knowledge of physical activity was found to be at least satisfactory in 90% of participants. Knowledge of the guidelines is insufficient in 75% of participants. The prescribing habits are inadequate in half of the participants. Full article
(This article belongs to the Special Issue Diabetes and Obesity: What Is New in 2025)
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10 pages, 413 KiB  
Protocol
V-CARE (Virtual Care After REsuscitation): Protocol for a Randomized Feasibility Study of a Virtual Psychoeducational Intervention After Cardiac Arrest—A STEPCARE Sub-Study
by Marco Mion, Gisela Lilja, Mattias Bohm, Erik Blennow Nordström, Dorit Töniste, Katarina Heimburg, Paul Swindell, Josef Dankiewicz, Markus B. Skrifvars, Niklas Nielsen, Janus C. Jakobsen, Judith White, Matt P. Wise, Nikos Gorgoraptis, Meadbh Keenan, Philip Hopkins, Nilesh Pareek, Maria Maccaroni and Thomas R. Keeble
J. Clin. Med. 2025, 14(13), 4429; https://doi.org/10.3390/jcm14134429 - 22 Jun 2025
Viewed by 485
Abstract
Background: Out-of-hospital cardiac arrest (OHCA) survivors and their relatives may face challenges following hospital discharge, relating to mood, cognition, and returning to normal day-to-day activities. Identified research gaps include a lack of knowledge around what type of intervention is needed to best navigate [...] Read more.
Background: Out-of-hospital cardiac arrest (OHCA) survivors and their relatives may face challenges following hospital discharge, relating to mood, cognition, and returning to normal day-to-day activities. Identified research gaps include a lack of knowledge around what type of intervention is needed to best navigate recovery. In this study, we investigate the feasibility and patient acceptability of a new virtual psychoeducational group intervention for OHCA survivors and their relatives and compare it to a control group receiving a digital information booklet. Methods: V-CARE is a comparative, single-blind randomized pilot trial including participants at selected sites of the STEPCARE trial, in the United Kingdom and Sweden. Inclusion criteria are a modified Rankin Scale (mRS) ≤ 3 at 30-day follow-up; no diagnosis of dementia; and not experiencing an acute psychiatric episode. One caregiver per patient is invited to participate optionally. The intervention group in V-CARE receives four semi-structured, one-hour-long, psychoeducational sessions delivered remotely via video call by a trained clinician once a week, 2–3 months after hospital discharge. The sessions cover understanding cardiac arrest; coping with fatigue and memory problems; managing low mood and anxiety; and returning to daily life. The control group receives an information booklet focused on fatigue, memory/cognitive problems, mental health, and practical coping strategies. Results: Primary: feasibility (number of patients consented) and acceptability (retention rate); secondary: satisfaction with care (Client Satisfaction Questionnaire 8 item), self-management skills (Self-Management Assessment Scale) and, where available, health-related outcomes assessed in the STEPCARE Extended Follow-up sub-study including cognition, fatigue, mood, quality of life, and return to work. Conclusions: If preliminary insights from the V-CARE trial suggest the intervention to be feasible and acceptable, the results will be used to design a larger trial aimed at informing future interventions to support OHCA recovery. Full article
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12 pages, 880 KiB  
Article
The Influence of Workplace-Integrated Exercise Snacks on Cognitive Performance in Sedentary Middle-Aged Adults—A Randomized Pilot Study
by Jonas P. Mues, Stefan Flohr and Nicolas Kurpiers
Sports 2025, 13(6), 186; https://doi.org/10.3390/sports13060186 - 13 Jun 2025
Viewed by 1158
Abstract
Sedentary behavior is increasingly recognized as a risk factor for various health issues, including cognitive decline. Objectives: This pilot study examined the acute and chronic effects of workplace-integrated exercise snacks—short, vigorous bouts of exercise—on cognitive performance in middle-aged adults. Methods: Twenty-five sedentary but [...] Read more.
Sedentary behavior is increasingly recognized as a risk factor for various health issues, including cognitive decline. Objectives: This pilot study examined the acute and chronic effects of workplace-integrated exercise snacks—short, vigorous bouts of exercise—on cognitive performance in middle-aged adults. Methods: Twenty-five sedentary but healthy office workers (51.4 ± 5.3 years; 6 m/19 f) participated. The intervention group (n = 12) performed three 1 min bouts of vigorous exercise (running on the spot) daily, four days a week, for four weeks, while the control group (n = 13) maintained their usual routine. Cognitive performance was assessed pre-intervention, shortly following the first exercise bout (acute effects), and post-intervention (chronic effects) using the Trail Making Test and the Stroop Color–Word Test. Results: Significant acute improvements (p ≤ 0.05) were observed across all cognitive outcomes following a single bout of exercise. Significant interaction effects (p ≤ 0.05) were found across all cognitive outcomes after four weeks, indicating sustained cognitive benefits. Conclusion: These findings suggest that workplace-integrated brief, vigorous exercise may lead to both immediate and sustained enhancement in executive functions such as working memory, processing speed, and inhibitory control. They highlight the potential cognitive health benefits of incorporating exercise snacks into sedentary workplace environments. Full article
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17 pages, 621 KiB  
Article
Joint Developmental Trajectories of Perinatal Depression and Anxiety and Their Predictors: A Longitudinal Study
by Minhui Jiang, Han Zheng, Zhaohua Bao, Zhenhong Wu, Xiaomin Zheng and Yaling Feng
Healthcare 2025, 13(11), 1251; https://doi.org/10.3390/healthcare13111251 - 26 May 2025
Viewed by 532
Abstract
Background: Perinatal depression and anxiety can be experienced simultaneously and change over time. This study aimed to explore the independent and joint developmental trajectories and predictors of perinatal depression and anxiety. Methods: From January 2022 to December 2023, a total of 1062 pregnant [...] Read more.
Background: Perinatal depression and anxiety can be experienced simultaneously and change over time. This study aimed to explore the independent and joint developmental trajectories and predictors of perinatal depression and anxiety. Methods: From January 2022 to December 2023, a total of 1062 pregnant women from Affiliated Women’s Hospital of Jiangnan University were surveyed for depression and anxiety symptoms using the Patient Health Questionnaire (PHQ-9) and Generalized Anxiety Disorder Scale (GAD-7) in early pregnancy (T1, 0–13+6 weeks), mid-term pregnancy (T2, 14–27+6 weeks), late pregnancy (T3, 28–41 weeks), and 42 days postpartum (T4). Parallel-process latent class growth model (PPLCGM) was performed to identify the joint developmental trajectories of perinatal depression and anxiety, and logistic regression was used to analyze factors of joint trajectories. Results: Perinatal depression and anxiety each showed four heterogeneous developmental trajectories, and three joint developmental trajectories were identified: “high–slightly decreasing depression and high decreasing anxiety group” (3%), “low–stable depression and low–stable anxiety group” (71%), and “moderate–slightly increasing depression and moderate–decreasing anxiety group” (26%). Adverse maternal history, history of anxiety and depression, and work stress were risk factors for the joint developmental trajectory of perinatal depression and anxiety, while regular exercise, paid work and social support were protective factors. Conclusions: Three joint developmental trajectories for perinatal depression and anxiety were identified, demonstrating group heterogeneity. Perinatal healthcare providers should pay attention to the mental health history of pregnant women, conduct multiple assessments of perinatal anxiety and depression, prioritize individuals with risk factors, and advocate for regular exercise, work participation, and provide greater social support. Full article
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13 pages, 1629 KiB  
Article
Differential Impact of Temperature, Release Rate, Prey Density, and Pesticides on Hyperaspis trifurcata (Coleoptera: Coccinellidae) to Optimize Integrated Management of Dactylopius opuntiae (Hemiptera: Dactylopiidae)
by Rachid Bouharroud, Salahddine Chafiki, Redouan Qessaoui, Yassine Imlil, Jamila Bargach, Aissa Derhem and Rachid Elaini
Plants 2025, 14(7), 1129; https://doi.org/10.3390/plants14071129 - 5 Apr 2025
Viewed by 2134
Abstract
The current work aims to establish an integrated pest management strategy using Hyperaspis trifurcata Schaeffer (Coleoptera: Coccinellidae) to control Dactylopius opuntiae Cockerell (Hemiptera: Dactylopiidae) and to assess the side effects of pesticides commonly used on this predator. The first part of this study [...] Read more.
The current work aims to establish an integrated pest management strategy using Hyperaspis trifurcata Schaeffer (Coleoptera: Coccinellidae) to control Dactylopius opuntiae Cockerell (Hemiptera: Dactylopiidae) and to assess the side effects of pesticides commonly used on this predator. The first part of this study was performed under controlled conditions at two temperatures with three prey densities and two release rates for 83 days. Under field conditions, a survival time test was conducted in a screen house (1.2 ha), where a total of 5700 predators were released on 1425 cactus plants and then monitored for a period of 23 weeks. Furthermore, eight pesticides were tested on H. trifurcata in laboratory conditions at five rates in order to define the lethal doses. Under controlled conditions, the effect of temperature on predation was not significant until 27 days after release. However, the prey density significantly impacted the predation rates from the 10th to 27th day after release (p < 0.001). The predator release rate significantly affected predation starting from the 15th day after release. The lowest median survival time based on Kaplan–Meier tests was obtained at 30 °C (the high temperature) for eight predators/cladode (27 days), but the highest was at 26 °C (the low temperature) for four predators/cladode (63 days). Depending on cochineal infestation, the effect of temperature significantly increased the predation rate from the 10th to 49th day after release, but only at a high density (50 colonies/cladode). Under field conditions, the effect of the infestation level on the survival function was significant (Log-Rank p < 0.05), and the median times were 111 and 130 days after release for low and high densities, respectively. Acetamiprid, Vaseline oil, black soap, copper oxychloride, and paraffin oil were highly toxic to H. trifurcata (>84% of mortality), and the LD50 values ranged from 2.3 to 69.6% of the recommended rate. For Mancozeb at the recommended dose, the mortality rate was low (<2%). The large-scale release of H. trifurcata would be successful in the Near East and North Africa (NENA) region, provided that the use of the mentioned pesticides is avoided or at least reduced. Full article
(This article belongs to the Special Issue Plant Protection and Integrated Pest Management)
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10 pages, 2280 KiB  
Communication
Changes in the Responses of Leaf Gas Exchange to Temperature and Photosynthesis Model Parameters in Four C3 Species in the Field
by James Bunce
Plants 2025, 14(4), 550; https://doi.org/10.3390/plants14040550 - 11 Feb 2025
Cited by 1 | Viewed by 821
Abstract
Substantial variation in the temperature dependence of parameters of the Farquhar, von Caemmerer, and Berry C3 photosynthesis model, as well as those of in vitro Rubisco kinetic characteristics, have been observed in controlled conditions but have seldom been systematically examined in the [...] Read more.
Substantial variation in the temperature dependence of parameters of the Farquhar, von Caemmerer, and Berry C3 photosynthesis model, as well as those of in vitro Rubisco kinetic characteristics, have been observed in controlled conditions but have seldom been systematically examined in the field. In this work, A vs. Ci curves were measured over a 15 or 20 °C range of temperature in four C3 species growing outdoors on two occasions about three weeks apart early in the growing season and also once near mid-season when air temperatures were more stable. The two early season occasions were chosen for having contrasting temperatures for 3 to 4 days preceding the measurements. Low temperatures (mean maximum/minimum temperatures of 19/11 °C) resulted in higher values of the VCmax of Rubisco and Jmax at a given measurement temperature in most species compared with higher temperatures (max/min 31/25 °C). The apparent activation energy of VCmax of Rubisco ranged from 56 to 82 kJ mol−1, and that of electron transport (Jmax) ranged from 28 to 56 kJ mol−1 across species and temperatures. In three of the four species, the activation energy of VCmax decreased and that of Jmax increased after the cooler temperatures. Stomatal conductance measured at 20 and 25 °C increased strongly with the prior warm temperatures in all species. Measurements made near mid-season, after a period of relatively stable temperatures (mean maximum/minimum temperatures of 27/18 °C), also indicated a wide range of values of the activation energies of VCmax and Jmax among these species. Full article
(This article belongs to the Special Issue Plant Photosynthesis in Complex Climates)
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20 pages, 3188 KiB  
Article
Volatile Organic Compounds in Early Childhood Education Facilities: Simultaneous Indoor and Outdoor Measurements in the Haifa Bay Area
by Ran Darzi, Merav Bareket, Yuval and Yael Dubowski
Atmosphere 2025, 16(2), 181; https://doi.org/10.3390/atmos16020181 - 5 Feb 2025
Viewed by 596
Abstract
Indoor air quality (IAQ) is of great importance, as people spend up to 90% of their time indoors, leading to significant exposure to air pollutants. The IAQ in early childhood education (ECE) facilities is of particular interest since young children are more vulnerable [...] Read more.
Indoor air quality (IAQ) is of great importance, as people spend up to 90% of their time indoors, leading to significant exposure to air pollutants. The IAQ in early childhood education (ECE) facilities is of particular interest since young children are more vulnerable and poor air quality may have possible long-lasting impacts on them. In the present study, simultaneous indoor and outdoor VOC measurements were carried out in three ECE facilities in the Haifa Bay area, Israel. Three sampling campaigns were utilized, each lasted for a minimum of one week, encompassing four consecutive working days and at least one weekend. During working days, sampling was performed during daytime activity hours and at nighttime (off hours). Twenty-three VOCs were identified, quantified, and classified into six chemical groups—aromatic hydrocarbons, aliphatic alkanes, terpenes, alcohols, carbonyls, and “others”. The total outdoor VOC concentration was 23 μg m−3 during the daytime and 22 μg m−3 at night, with carbonyls and aromatic hydrocarbons accounting for ~80% of it. Despite the heterogeneity of the study area, outdoor concentrations depicted a smaller spatial and temporal variability than was observed indoors. In the ECE facilities, the total VOC reached 134 and 204 μg m−3 during the daytime and nighttime, respectively, and were strongly impacted by the air exchange rate. Carbonyls, alcohols, and terpenes were more prevalent indoors, accounting for 77.5–81.1% of the total. Their high indoor/outdoor ratios, especially for formaldehyde and limonene, suggest a significant contribution from indoor emission sources. Exposure calculations were compared to reference values for carcinogenic and non-carcinogenic effects. While the lifetime average daily dose (LADD) did not exceed the available reference values, the upper-limit estimates of continuous lifetime exposure to measured indoor levels indicate that formaldehyde and acetaldehyde surpassed their respective limits by factors of 10 and 3, respectively. Full article
(This article belongs to the Section Air Quality)
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13 pages, 1547 KiB  
Article
Protective and Therapeutic Effects of Orlistat in Combination with Elettaria cardamomum “Cardamom” Extract on Learning, Memory, Anxiety, and Neuroinflammation in Obese Mice
by Anfal AL-Dalaeen, Nour Batarseh, Nadine N. Abdelhadi, Sally Atawneh, Reem AbuKashef and Ali Mosa Ra-shid Al-Yasari
Medicina 2025, 61(2), 263; https://doi.org/10.3390/medicina61020263 - 4 Feb 2025
Cited by 1 | Viewed by 1438
Abstract
Introduction and Objective: Obesity has increased worldwide, and existing anti-obesity medications have treatment limitations that diminish their overall benefits. This study aimed to investigate the effects of orlistat in combination with Elettaria cardamomum “Cardamom” (CAR) extract on working memory, recognition memory, anxiety, [...] Read more.
Introduction and Objective: Obesity has increased worldwide, and existing anti-obesity medications have treatment limitations that diminish their overall benefits. This study aimed to investigate the effects of orlistat in combination with Elettaria cardamomum “Cardamom” (CAR) extract on working memory, recognition memory, anxiety, and inflammation within hippocampal tissue. Methods: Mice were categorized into two groups: a control group (CD) and a cafeteria diet (CAF) group induced with obesity (CAF) for 10 weeks. The groups were then subdivided into a CAF group treated with orlistat (CAF-ORL), a CAF group treated with orlistat and Elettaria cardamomum (CAF-ORL-CARD), and a group that continued on the CAF. The CAF-ORL group received orlistat at a dosage of 10 mg/kg/day for four weeks, while the CAF-ORL-CARD group received 10 mg/kg/day of orlistat and 500 mg/kg of CAR extract via oral gavage. In the 14th week, various assessments were conducted, including the novel object recognition (NOR) test, Y maze test, marble-burying test (MBT), open-field test, and TNF-α levels in the hippocampus. Result: TNF-α levels in the hippocampal tissue of the CAF group were elevated compared to the CD group (p < 0.01), whereas the CAF-ORL group exhibited reduced TNF-α levels compared to the CAF group (p < 0.01). Moreover, TNF-α levels in the CAF-ORL-CARD group were significantly lower than in the CAF-ORL group (p < 0.01). The recognition index was notably higher in the CAF-ORL group compared to the CAF group (p < 0.01) and higher in the CAF-ORL-CARD group compared to the CAF-ORL group (p < 0.01). However, there were no changes in the open-field test and Y maze test (p > 0.05). Conclusions: Orlistat combined with CAR has positive effects on neuroinflammation and memory, suggesting that this combination may offer potential therapeutic benefits for cognitive impairments and hippocampal dysfunction associated with obesity. Full article
(This article belongs to the Section Pharmacology)
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14 pages, 506 KiB  
Article
Multidomain Cognitive Tele-Neurorehabilitation Training in Long-Term Post-Stroke Patients: An RCT Study
by Marianna Contrada, Gennarina Arabia, Martina Vatrano, Caterina Pucci, Isabel Mantia, Federica Scarfone, Giusi Torchia, Maria Quintieri, Antonio Cerasa and Loris Pignolo
Brain Sci. 2025, 15(2), 145; https://doi.org/10.3390/brainsci15020145 - 31 Jan 2025
Cited by 4 | Viewed by 1421
Abstract
Purpose: Over the past decade, tele-neurorehabilitation (TNR) has emerged as a vital and effective tool for delivering continuous care to stroke patients, playing a key role in enhancing functional recovery and ensuring consistent access to rehabilitation services. In the field of TNR, various [...] Read more.
Purpose: Over the past decade, tele-neurorehabilitation (TNR) has emerged as a vital and effective tool for delivering continuous care to stroke patients, playing a key role in enhancing functional recovery and ensuring consistent access to rehabilitation services. In the field of TNR, various protocols are utilized to ensure effective cognitive stimulation at home. Recent preliminary studies highlight the employment of multidomain cognitive interventions, which would seem to induce more stable and relevant cognitive recovery in stroke patients. A randomized controlled trial (RCT) study was conducted to compare the effectiveness of a TNR multidomain cognitive approach to conventional face-to-face cognitive treatment. Methods: A total of 30 patients with stroke were equally enrolled and randomly assigned to the experimental and control groups. In the experimental group, patients received sessions of home-based cognitive virtual reality rehabilitation system (VRRS) training. The control group underwent traditional face-to-face cognitive multidomain treatment at the hospital. The therapy was given for one hour every day for four weeks in both groups. Specific cognitive domains, including memory, praxis skills, executive functions, and speech therapy, were stimulated in the procedure. Neuropsychological evaluations were performed at three timepoints: at baseline (T0), at the end of TNR (T1), and six months later (T2). Results: The TNR group demonstrated significant improvements in working memory and language abilities, as well as in depressive symptoms and caregiver burden, with an average decrease of 2.07. Most of this improvement persisted 6 months after treatment. The group that received face-to-face cognitive treatment showed improvements (not persisting at T2) after treatment in a task measuring constructive apraxia and alternating attention with the cognitive skill of set-shifting. Conclusions: According to our findings, multidomain cognitive TNR may be useful in enhancing cognitive outcomes in stroke populations (even six months after treatment concludes). TNR may also be a viable way to deliver these interventions since it boosts people’s motivation to train and, consequently, their adherence to treatment while also having a positive effect on caregivers’ distress management. Full article
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17 pages, 3549 KiB  
Article
Wastewater-Based Epidemiological Surveillance in France: The SUM’EAU Network
by Frédéric Jourdain, Laila Toro, Zoé Senta-Loÿs, Marilyne Deryene, Walid Mokni, Tess Azevedo Da Graça, Yann Le Strat, Sofiane Rahali, Ami Yamada, Anna Maisa, Maël Pretet, Jeanne Sudour, Christophe Cordevant, Thierry Chesnot, Veronica Roman, Amandine Wilhelm, Benoît Gassilloud and Damien Mouly
Microorganisms 2025, 13(2), 281; https://doi.org/10.3390/microorganisms13020281 - 26 Jan 2025
Cited by 2 | Viewed by 2035
Abstract
Wastewater surveillance is a powerful public health tool which gained global prominence during the COVID-19 pandemic. This article describes the development and implementation of the national wastewater surveillance network in France: SUM’EAU. Preliminary work included defining a sampling strategy, evaluating/optimising analytical methods, launching [...] Read more.
Wastewater surveillance is a powerful public health tool which gained global prominence during the COVID-19 pandemic. This article describes the development and implementation of the national wastewater surveillance network in France: SUM’EAU. Preliminary work included defining a sampling strategy, evaluating/optimising analytical methods, launching a call for tenders to select network laboratories and producing wastewater monitoring indicators. SUM’EAU was then deployed in three stages: (i) a pilot study, (ii) the transfer of analytical activities from the National Reference Laboratory to four selected network laboratories, and (iii) the extension of the system to additional sampling sites. Currently, SUM’EAU monitors SARS-CoV-2 across 54 wastewater treatment plants in mainland France. Once a week on business days, 24 h flow-proportional composite samples are collected at plant inlets and transported at 5 °C (±3 °C) to partner laboratories for analysis. The analytical process involves sample concentration, RNA extraction, and digital RT-PCR/q-RT-PCR to detect and quantify the presence of the SARS-CoV-2 genome in wastewater. Subsequently, data are transferred to Santé publique France, the French National Public Health Agency, for analysis and interpretation. While SUM’EAU has been instrumental in monitoring the COVID-19 pandemic and holds significant potential for broader application, securing sustainable funding for its operation remains a major challenge. Full article
(This article belongs to the Special Issue Surveillance of SARS-CoV-2 Employing Wastewater)
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10 pages, 235 KiB  
Case Report
Acute Febrile Illness Accompanied by 7th and 12th Cranial Nerve Palsy Due to Lyme Disease Following Travel to Rural Ecuador: A Case Report and Mini-Review
by Teslin S. Sandstrom, Kumudhavalli Kavanoor Sridhar, Judith Joshi, Ali Aunas, Sheliza Halani and Andrea K. Boggild
Trop. Med. Infect. Dis. 2025, 10(1), 21; https://doi.org/10.3390/tropicalmed10010021 - 14 Jan 2025
Viewed by 1459
Abstract
The causative agent of Lyme disease, Borrelia burgdorferi, is endemic to Canada, the northeastern United States, northern California, and temperate European regions. It is rarely associated with a travel-related exposure. In this report, we describe a resident of southern Ontario, Canada who [...] Read more.
The causative agent of Lyme disease, Borrelia burgdorferi, is endemic to Canada, the northeastern United States, northern California, and temperate European regions. It is rarely associated with a travel-related exposure. In this report, we describe a resident of southern Ontario, Canada who developed rash, fever, and cranial nerve VII and XII palsies following a 12 day trip to Ecuador and the Galapagos islands approximately four weeks prior to referral to our center. Comprehensive microbiological work-up was notable for reactive Borrelia burgdorferi serology by modified two-tier testing (MTTT), confirming a diagnosis of Lyme disease. This case highlights important teaching points, including the classic clinical presentation of acute Lyme disease with compatible exposure pre-travel in a Lyme-endemic region of Ontario, initial manifestations during travel following acquisition of arthropod bites in Ecuador, and more severe manifestations post-travel. Given the travel history to a South American country in which Lyme disease is exceedingly uncommon, consideration of infections acquired in Ecuador necessitated a broad differential diagnosis and more comprehensive microbiological testing than would have been required in the absence of tropical travel. Additionally, cranial nerve XII involvement is an uncommon feature of Lyme neuroborreliosis, and therefore warranted consideration of an alternative, non-infectious etiology such as stroke or a mass lesion, both of which were excluded in this patient through neuroimaging. Full article
(This article belongs to the Special Issue Emerging Trends of Infectious Diseases in Canada)
14 pages, 2414 KiB  
Article
Effects of Photopatterning Conditions on Azimuthal Surface Anchoring Strength
by Nilanthi P. Haputhanthrige, Mojtaba Rajabi and Oleg D. Lavrentovich
Crystals 2024, 14(12), 1058; https://doi.org/10.3390/cryst14121058 - 6 Dec 2024
Cited by 2 | Viewed by 1187
Abstract
Spatially varying alignment of liquid crystals is essential for research and applications. One widely used method is based on the photopatterning of thin layers of azo-dye molecules, such as Brilliant Yellow (BY), that serve as an aligning substrate for a liquid crystal. In [...] Read more.
Spatially varying alignment of liquid crystals is essential for research and applications. One widely used method is based on the photopatterning of thin layers of azo-dye molecules, such as Brilliant Yellow (BY), that serve as an aligning substrate for a liquid crystal. In this study, we examine how photopatterning conditions, such as BY layer thickness (b), light intensity (I), irradiation dose, and age affect the alignment quality and the strength of the azimuthal surface anchoring. The azimuthal surface anchoring coefficient, W, is determined by analyzing the splitting of integer disclinations into half-integer disclinations at prepatterned substrates. The strongest anchoring is achieved for b in the range of 5–8 nm. W increases with the dose, and within the same dose, W increases with I. Aging of a non-irradiated BY coating above 15 days reduces W. Our study also demonstrates that sealed photopatterned cells filled with a conventional nematic preserve their alignment quality for up to four weeks, after which time W decreases. This work suggests the optimization pathways for photoalignment of nematic liquid crystals. Full article
(This article belongs to the Special Issue Liquid Crystal Research and Novel Applications in the 21st Century)
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12 pages, 488 KiB  
Article
Mobile App Intervention Increases Adherence to Home Exercise Program After Whiplash Injury—A Randomized Controlled Trial (RCT)
by Blaž Barun, Zdravko Divić, Dušanka Martinović Kaliterna, Ana Poljičanin, Benjamin Benzon and Jure Aljinović
Diagnostics 2024, 14(23), 2729; https://doi.org/10.3390/diagnostics14232729 - 4 Dec 2024
Cited by 1 | Viewed by 1739
Abstract
Objective: Can mobile app intervention via push notifications increase adherence to exercise and reduce disability and pain after a whiplash injury? Methods: A randomized controlled trial was conducted with concealed allocation, blinding of some assessors, and an intention-to-treat analysis. Participants who sustained whiplash [...] Read more.
Objective: Can mobile app intervention via push notifications increase adherence to exercise and reduce disability and pain after a whiplash injury? Methods: A randomized controlled trial was conducted with concealed allocation, blinding of some assessors, and an intention-to-treat analysis. Participants who sustained whiplash injury at most 3 months prior were divided into active and control groups. Both groups completed a two-part physiotherapist-supervised physical therapy program (3-week break in between, ten sessions each, 5x/week). The program included TENS, therapeutic ultrasound, and exercises (breathing, ROM, deep neck flexor activation, and stretching). Both groups were encouraged to exercise at home. The active group additionally received push notifications through the mobile app once a day as a reminder to exercise. Outcomes were adherence to exercise (four-point Likert scale), physical functioning (NDI), pain intensity (VAS), perceived recovery (three-point Likert scale), work information, psychological functioning (PCS), and HRQoL (SF-12) at baseline and 6-month follow-up. Results: At month 6, when comparing the groups, the intervention group showed higher adherence to home exercise (3 [2–4] vs. 2 [2–4]; p = 0.005, median [IQR]) and improved HRQoL (∆SF-12) (20 [6–36] vs. 15 [9–23]; p = 0.038). Unlike the control group, the intervention group showed a significant decrease in pain catastrophizing (31%; p = 0.01). A multivariant analysis showed that mobile app intervention influenced adherence most (≈1 Likert point). The groups did not differ in NDI, pain VAS, perceived recovery, or work limitation. Conclusions: Mobile app intervention increased adherence to home exercise, reduced pain catastrophizing, and increased HRQoL six months after a whiplash injury. Trial registration: ClinicalTrials.gov NCT05704023. Full article
(This article belongs to the Special Issue Diagnosis and Management of Postural Disorders)
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Article
Chronic and Acute Pain and Adverse Economic Outcomes in a 9/11-Exposed Cohort
by Jennifer Brite, Junaid Maqsood, Shengchao Yu, Howard E. Alper and James E. Cone
Int. J. Environ. Res. Public Health 2024, 21(11), 1490; https://doi.org/10.3390/ijerph21111490 - 9 Nov 2024
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Abstract
The objective of this study was to determine the association between acute and chronic pain and various economic and quality-of-life outcomes. The study sample was drawn from the World Trade Center Health Registry. Both acute and chronic pain were measured in 2020–2021. Logistic [...] Read more.
The objective of this study was to determine the association between acute and chronic pain and various economic and quality-of-life outcomes. The study sample was drawn from the World Trade Center Health Registry. Both acute and chronic pain were measured in 2020–2021. Logistic regression models were constructed to determine the odds of several economic and quality-of-life variables: early retirement, low household income, decline in household income, quality of life, and not working due to health. Those who had extreme pain in the last four weeks that interfered with normal work had 3.12 (95% confidence interval (CI): 2.36, 4.39) times the odds of early retirement, 5.34 (95% CI: 3.47, 8.21) times the odds of having a household income below USD 50,000, 2.56 (95% CI: 1.51, 4.33) times the odds of having an income decrease, and 14.4 (95% CI: 11.3, 18.3) times the rate of poor health days compared to those with no pain. Similar results were found for chronic pain. The detrimental effects of pain may influence multiple facets of a patient’s life, and both financial and overall well-being may diminish even several years after a pain diagnosis. Full article
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