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Background: The lack of a short-course of safe and effective macrofilaricidal therapy for lymphatic filariasis (LF) hinders elimination efforts, especially in the endgame scenario. Preclinical studies in mice demonstrated that high-dose rifampicin (RIF) plus albendazole (ALB) produced macrofilaricidal effects within seven days, prompting this randomised, open-label, parallel-group, interventional phase II pilot trial to determine the efficacy of high-dose RIF plus ALB against LF in humans. Methods: In three LF-endemic districts of Ghana’s Upper East Region, circulating filarial antigen (CFA)-positive individuals aged 18 to 55 years identified using the Alere Filariasis Test Strip were enrolled into the study. The participants were randomised through a centralized computer-generated randomisation into four treatment arms. They were treated according to the arm they were assigned to and followed up at 4-, 6-, 12-, and 18-months post-treatment to monitor changes in CFA status and levels, as well as adverse events. Outcome assessors were blinded to minimize assessment bias. Results: A total of 69 eligible participants were randomised into four treatment arms: RIF (35 mg/kg/day) + ALB (400 mg/day) for 7 days (n = 17), RIF (35 mg/kg/day) + ALB (400 mg/day) for 14 days (n = 18), ALB alone for 14 days (n = 17), and an untreated controlled group participating in standard mass drug administration (n = 17). All regimens were well tolerated, with no serious adverse events. Even though CFA positivity declined across all groups, with maximal reductions at 18 months, the RIF + ALB 7-day regimen consistently showed the highest decline, while ALB alone was the least effective. RIF + ALB groups exhibited early antigen decline by 4 months, unlike comparator groups, where reductions occurred from 12 months. Conclusions: These findings suggest macrofilaricidal activity of high-dose RIF plus ALB, supporting further trials in larger, microfilaraemic populations. The trial was registered in the Pan African Clinical Trials Registry on 9 September 2020 under the code PACTR202009704006025.Funding was by the European and Developing Countries Clinical Trials Partnership 2 (EDCTP2), with grant code TMA2018SF-2451-ASTAWOL, and by the German Federal Ministry of Education and Research (Bundesministerium fur Bildung und Forschung-BMBF) under agreement with Gesellschaft für Internationale Zusammenarbeit (GIZ) through agreement number: 81204851. Full article

Human embryonic kidney (HEK293) cells are a widespread choice for recombinant protein expression. To optimise yields, the hydrolysate Tryptone N1 (TN1) is commonly added post-transfection. TN1 is obtained by controlled enzymatic digestion of casein. As an animal by-product, TN1 faces stricter regulations during cross-country shipments than plant-based products. This raises the question of whether plant-derived peptides are a suitable alternative to TN1. Using polyethyleneimine (PEI) as a cationic polymer, we transfected HEK293-6E cells grown in suspension in serum-free medium and divided the transfectants into four groups (each in triplicate). Two plant-based hydrolysates each derived from pea and broad bean were compared with TN1 and a no-hydrolysate control group. We monitored the cultures for total cell numbers and viability at days 1, 4, and 5 post-transfection. Both plant-based hydrolysates and TN1 showed similar live cell percentages, in contrast to the no-hydrolysate control, which showed lower viability. Five days post-transfection, the expressed His-tagged protein, a tegumental antigen from the eukaryotic parasite Echinococcus granulosus, was retrieved from the serum-free culture supernatant, and the expressed recombinant protein was quantified. The linear ranges for the protein load on the stain-free blot and for the use of the fluorescent anti-His-Tag Alexa₄₈₈ antibody were determined. Using these parameters, stain-free Western blotting and total protein normalization were performed. The plant-derived pea and broad bean hydrolysates reproducibly resulted in similar expression levels as animal-derived TN1; all three hydrolysates were better than no hydrolysate. We conclude that plant-derived hydrolysates are a suitable, more sustainable replacement for TN1. Full article

The way in which remote working is interpreted by organizations (by top management, by human resources management), even in its being an element of possible sustainability, may vary in relation to national work cultures and laws, with differences between countries. This study, in particular, aims to trace the differences and similarities in terms of how organizations regulate and manage remote working by considering 294 organizations of four different countries: France (n = 92), Switzerland (n = 82), Italy (n = 60), and Quebec (n = 60). In each country, each response relates to an entire organization. The findings show that perceived pressure from the environment to implement remote working is higher in Quebec (Canada) than in Switzerland and Italy. In terms of regulations, 90% of Quebec organizations reported to have approved an internal regulation for remote working compared with European countries, ranging between 72% (for Italy) and 76% (for France). Finally, the findings confirm the role played by top-management positive beliefs for remote working and pressure from the environment for the implementation of different adaptations: All these findings show differences in particular between Quebec and European countries that are interesting to better understand and debate. Full article

Timely identification of infectious disease threats is essential for public health readiness. Conventional indicator-based surveillance systems, while dependable for tracking established pathogens, frequently lack the agility and sensitivity to detect new infections promptly. Syndromic surveillance, which examines pre-diagnostic and non-specific health indicators from many data sources in near real time, serves as a significant complementary method that improves early warning and situational awareness. This narrative study analysed selected experiences with syndromic surveillance, utilising peer-reviewed literature and institutional records. Four primary data streams were examined: emergency department and hospital records, pharmacy and over the counter (OTC) sales, participative citizen-generated data, and hybrid multi-source systems. Syndromic indicators were reported to identify outbreaks two to fourteen days before standard laboratory reporting across many trials. Data from the emergency department exhibited the highest sensitivity and specificity (47.34% and 91.95%, respectively), whereas pharmacy and participative data offered early indicators at the community level. Integrated systems like ESSENCE (Johns Hopkins University Applied Physics Laboratory, Laurel, MD, USA) and SurSaUD^® (Saint-Maurice cedex, Paris, France) attained enhanced accuracy yet necessitated significant data integration and governance capabilities. Syndromic surveillance enhances epidemic preparation by detecting atypical health-seeking behaviours and variations from baseline patterns prior to formal diagnosis. Nonetheless, its efficacy is contingent upon data quality, interoperability, and contextual adaptation. Countries like Bulgaria could improve national early-warning capabilities and overall health security through the gradual adoption of pilot projects and integration with existing surveillance networks. Full article

This study aimed to identify young school students’ language learning strategies (LLSs) within their diverse socio-linguistic reality. The study was conducted in one elementary school in a peripheral city characterized by a heterogeneous population (Arabs and Jews) and immigrants from various countries who speak multiple languages. The principal of this school opted to introduce young children (Grades 1 and 2) to four languages: Hebrew, as a socially dominant language; Russian and Arabic, as the children’s home languages; and English, as a global language. We used photo elicitation and dialogical conversation to obtain reflections of 11 Arab and Jewish students (Grade 2). Each student was asked to describe the strategies they used to learn a novel language in the classroom and at home. Findings support the appropriateness of Oxford’s taxonomy to young language learners: all LLSs’ categories were reported. This study contributes to our understanding of children’s ability to use LLSs in early primary school. It highlights the leading role of language teachers who seem to mediate by modelling LLSs. Furthermore, it enriches the understanding of how 7–8-year-old learners can use diverse metacognitive LLSs and transfer them across languages. We also found one “child-specific” characteristics of the strategy related to parental involvement. Full article

(1) Background: Regulations governing food supplements vary considerably across countries, allowing products that are prohibited in one jurisdiction to be legally sold in another. Furthermore, online sales enable and facilitate this practice. Regarding pharmaceutical malpractice, the absence of a standardized European framework complicates the evaluation of pharmacist liability. As a result, the specific elements of the liability framework are defined by the national legislation of each Member State. The aim of our review is to map the global regulatory landscape of food supplements and to examine the pharmacist’s professional responsibilities, including instances of malpractice related to this area. (2) Methods: A literature review covering publications from January 2020 to December 2024 was performed using four databases: Scopus, PubMed, Embase, and Web of Science. The search retrieved 8243 records, of which 77 studies fulfilled the eligibility criteria. The extracted data were organized into five main themes: pharmacist responsibility and malpractice, food supplement regulation, consumer safety, health claims, and pharmacist knowledge. (3) Results: The literature reviewed indicated a relatively low number of malpractice cases within the pharmacy profession compared to other professions. A higher incidence of cases is observed among male pharmacists and those practicing in the private sector. Notably, no cases have been identified addressing pharmacists’ responsibilities in the dispensing of food supplements. In the context of food supplement regulation, the reviewed literature highlights a lack of standardized terminology and harmonized legislation across different jurisdictions. Therefore, products may be classified differently across jurisdictions. Another observed barrier is the considerable variation in market access requirements across countries. Regarding consumer safety, several irregularities have been observed. Substantial non-compliance in both product composition and labeling has been observed, reflecting insufficient quality control measures. Concerning health claims, significant regulatory non-compliance with European Union regulations has been documented. In addition, widespread misleading advertising practices have been observed. With respect to pharmacists’ knowledge, the reviewed literature identifies several professional challenges within pharmacy practice, particularly those concerning the dispensing of food supplements. (4) Conclusions: This research offers a comprehensive analysis of the literature published over the past five years concerning pharmaceutical malpractice cases, as well as an examination of food supplement regulation and the professional responsibilities of pharmacists. A recurring barrier identified is the absence of unified regulatory frameworks worldwide. This results in uncertainty concerning the pharmacist’s professional role and responsibilities. Full article

Measuring national progress toward green transformation remains challenging due to fragmented assessment frameworks. This study develops and validates a Green Transformation Index that captures the capacity for sustainability transitions by integrating resource efficiency, innovation systems, and policy instruments. Using OECD Green Growth Indicators covering 58 economies from 2017 to 2025, we construct a composite index from 47 standardized indicators organized into three theoretically grounded dimensions. The GTI measures transformation capacity through innovation investment and policy frameworks rather than environmental outcomes. Results reveal substantial heterogeneity in transformation capacity with a Gini coefficient of 0.283, indicating persistent global inequality. Temporal analysis identifies a three-phase trajectory: consolidation from 2017 to 2019, acceleration during 2021 to 2023 driven by green recovery investments, and marked reversal in 2024 to 2025, highlighting vulnerability to economic shocks. Cluster analysis identifies four distinct pathways: innovation-driven, balanced integration, resource-first, and policy-led approaches. Critical findings show only 19 percent of countries demonstrate strong coordination between innovation investments and policy instruments, revealing significant governance fragmentation. Validation tests confirm the index effectively measures innovation capacity but shows weak correlation with emissions outcomes, underscoring the distinction between transformation inputs and environmental performance. Full article

This study develops a Smart Mobility Index (SMI) tailored to the 17 Local Government Units (LGUs) of Metro Manila to evaluate their readiness to adopt integrated, efficient, and technology-enabled mobility systems. While global smart mobility indices are often ill-suited to the realities of developing countries, this research proposes a context-specific framework built around four thematically grounded dimensions: public transportation service, active mobility, unified cashless fare systems, and smart traffic management. The SMI was constructed through a mixed-method approach combining expert interviews with metropolitan transport specialists and co-occurrence network analysis. The results reveal substantial disparities across LGUs, with central jurisdictions such as Makati, Manila, and Pasay demonstrating significantly higher smart mobility readiness than peripheral LGUs. Clustering identifies three distinct mobility profiles, underscoring persistent structural inequalities in infrastructure, institutional capacity, and digital integration. Forecasts incorporating the completion of six major railway projects by 2035 indicate moderate improvements in overall SMI scores and limited changes in relative rankings, suggesting that infrastructural expansion alone will not reduce regional disparities. Expert insights further highlight both the potential and the constraints of leapfrogging, with interviewees expressing optimism regarding advanced ICT-enabled mobility solutions while acknowledging challenges related to governance fragmentation, limited funding, and uneven technical capabilities. Full article

Background: Rice is one of the world’s main staple crops, and improving its productivity and resilience is important to achieving food security under varying climatic conditions. Objectives: This systematic review synthesizes the existing evidence on the application, technical limitations, and potential of the development of genome editing technologies (CRISPR-Cas) in rice (Oryza sativa L.), as well as presents a novel approach called the CRISPR Trait Prioritization and Readiness Framework (CTPRF). Methods: Peer-reviewed articles that reported applications of genome editing based on the CRISPR-Cas system in the genome of rice for trait improvement or functional genomics were identified through searches fromPubMed, Scopus, Web of Science, and Google Scholar with studies published between 2012 and 2025. Studies were screened on predefined inclusion criteria related to experimental validation, reporting of editing efficiency, and clear phenotypic results. Data on CRISPR systems, target genes, methods of delivery, traits modified, and phenotypic results were extracted and synthesized by comparative analysis. Results: A wide variety of different CRISPR systems have been used in rice, and our results indicate that NHEJ-mediated knockouts are effective in average genotypes with editing efficiencies in the range of 70–90%, but HDR and prime editing are still under 10%. The CTPRF is being introduced as a strategic decision support tool to evaluate traits from four dimensions: technical feasibility, phenotypic predictability, impact potential, and regulatory pathway. We use this framework for case studies in pioneering countries (USA, Japan, China) and show how it can be useful for guiding research investment and policy. Conclusions: CRISPR-Cas technologies have transformed rice breeding, but their introduction requires overcoming genotype-dependent barriers to transformation and negotiating patchwork regulatory environments. The CTPRF offers a roadmap for the acceleration of the development of climate-resilient and nutritious rice varieties for the action plan. Full article

The global construction industry faces persistent challenges of low productivity and labor shortages, positioning Off-Site Construction (OSC) as a critical solution. However, standardized industrialization indices for objectively evaluating OSC adoption remain underdeveloped, particularly in emerging markets. This study aims to identify a benchmark policy model and derive design principles for future indices. Specifically, this study focuses on ‘policy-driven markets’ where strong government intervention is essential for initial ecosystem formation, excluding mature market-driven economies where the ecosystem is already established (e.g., USA, Sweden, Japan). To identify an optimal benchmark, a comparative assessment was conducted on five institutional frameworks across four countries (UK, Malaysia, Singapore, and China). Notably, within China, Hong Kong SAR was analyzed as a distinct regulatory jurisdiction separate from Mainland China due to its unique construction governance system. This assessment was based on five key policy dimensions: Legal Mandate, Scope, Indicator Composition, Enforcement Mechanism, and Sustainability. The analysis identified Singapore’s ‘Buildability Score’ as the most comprehensive model in terms of systemic completeness and practical efficacy. A virtual project simulation demonstrated that the scoring system functions as a powerful regulatory mechanism, effectively driving the adoption of standardized, dry-process, and modularized high-productivity methods from the earliest design stages. While Singapore’s system serves as an effective policy tool for OSC proliferation, it exhibits clear limitations regarding reduced architectural design flexibility and insufficient sustainability integration. Consequently, future industrialization indices must evolve to balance productivity with architectural design diversity and integrate sustainability criteria while reflecting specific regional construction ecosystems. Full article

Estimating nitrogen (N) and the corresponding crude protein (CP) content in forage crops is essential for optimizing fertilization and livestock nutrition. However, standard methods such as the Dumas and Kjeldahl techniques are destructive, costly, and impractical for field use in certain regions of developing countries. This study evaluated four non-destructive approaches—morphometric measurements, Pantone^® color scales, smartphone-based RGB analysis (ColorDetector app), and SPAD chlorophyll readings—for predicting N and CP in Megathyrsus maximus (Mombasa grass). A total of 120 samples were collected under three nitrogen fertilization levels and assessed using linear mixed-effects models with cross-validation. Morphometric variables showed poor performance (R² < 0.01), indicating low correlation with nutrient content. Pantone-based RGB models provided slightly better predictions (R² ≈ 0.30) but were limited by subjectivity and discrete data. SPAD-based models demonstrated moderate predictive accuracy (R² ≈ 0.53; RMSE ≈ 0.46%). The highest accuracy was achieved with smartphone-derived RGB data, where full RGB models reached R² = 0.60 and RMSE = 0.45%. Based on these results, a practical green color scale was developed from RGB values to support real-time, in-field nitrogen and crude protein assessment. This study highlights smartphone imaging as a scalable, low-cost, and accurate tool for non-destructive estimation of nitrogen and crude protein in tropical forages, offering an accessible alternative to laboratory methods for producers and field technicians. Full article

Background: Neonatal mortality remains a significant public health challenge in Ethiopia. Despite efforts to implement key evidence-based interventions, their coverage and utilization remain low. The Saving Little Lives (SLL) program aims to scale-up a Minimum Care Package (MCP) of synergistic, life-saving interventions for all liveborn neonates, with a focus on preterm and low birth weight (LBW) infants, across 290 hospitals in Ethiopia (206 primary, 69 general, and 15 referral hospitals), representing 82% of all hospitals in the country at the time of the study, and evaluate the impact on neonatal mortality. Methods: A non-randomized stepped-wedge trial will be conducted to evaluate the impact of implementing the SLL MCP interventions. Quantitative evaluation data will be collected from 36 primary hospitals, selected from 206 primary hospitals across four regions, receiving the interventions. An independent evaluation research assistant will be deployed in each of the hospitals to collect data using Open Data Kit (ODK) through interviewing mothers before discharge, on the 29th day of life if discharged, and reviewing medical records. A mixed-method, cross-sectional formative assessment will be conducted prior to implementation, employing quantitative facility assessment and qualitative interviews with mothers, healthcare providers, and facility managers. This will be followed by continuous program learning assessment once implementation begins. Descriptive data will be presented using numbers, percentages, tables, and graphs. Regression modeling and generalized estimating equations (GEEs) will be used to estimate the impact of the SLL MCP interventions. Qualitative data will be gathered through in-depth interviews, digitally recorded, transcribed, and thematically analyzed using ATLAS.ti Version 7.5 software to assess facility readiness, barriers, and enablers of implementing the SLL MCP interventions. Expected Outcome: We hypothesize that achieving 80% coverage of the SLL MCP interventions among eligible neonates will result in a 35% reduction in neonatal mortality at implementation facilities. Full article

Slovakia’s biomethane production potential represents up to 10% of Slovakia’s natural gas consumption, which is largely unexploited. The aim of this paper is to develop a model of each available technology (continuous, dry batch, and wet batch) as well as that of a biogas treatment unit and evaluate the energetic, economic, and environmental potential of building a new anaerobic digestion plant in Slovakia, considering four plant locations with feedstock abundance within a 30 km perimeter. Feedstock composition and availability, energy integration, and product usability are evaluated. The applied multi-criteria decision analysis (MCDA) considers four evaluation criteria: return on investment (ROI), CO₂ emissions production, potential industrial biowaste revenue, and municipal density within the operational region. Biogas plant deployment analysis yielded the Levice facility as top-ranked, primarily due to its minimal environmental impact and superior logistical performance, closely followed by the Žilina, Michalovce, and Prešov facilities. When comparing biomethane production facilities, the Levice plant was excluded due to economic infeasibility, and the Žilina facility emerged as the optimal choice, particularly due to its superior ROI performance and the largest biomethane production potential of over 1 million m³ biomethane per year. Thus, biomethane station deployment in Slovakia has proved feasible and can enhance the energy self-sustainability of the country and contribute to meeting the decarbonization goals. Full article

Background/Objectives: Shigella is the leading bacterial cause of diarrheal disease worldwide. Although multiple vaccine candidates are under development and in clinical trials, no Shigella vaccine is currently available on the market. Shigella comprises four species: S. dysenteriae, S. flexneri, S. boydii, and S. sonnei. S. flexneri has been recognized as the most prevalent species, particularly in low- and middle-income countries (LMICs), and the top serotypes are S. flexneri 2a, 3a and 6. Conversely, S. sonnei has a single serotype and predominates in high-income countries (HICs). Invasion plasmid antigen B (IpaB) is a critical virulence factor of Shigella type III secretion system (T3SS) that is highly conserved across Shigella serotypes. Here, we report the development of a Shigella quadrivalent O-polysaccharide-IpaB conjugate vaccine candidate (IVT Shigella-04). Methods: IVT Shigella-04 contains O-polysaccharides (O-PS) from S. flexneri 2a, 3a, 6, and S. sonnei, each individually conjugated to recombinantly expressed IpaB as the carrier protein using 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP) chemistry. The immunogenicity of IVT Shigella-04 was evaluated in a rabbit immunization model. Results: Baseline (day 0) IgG concentrations were low for all four Shigella serotypes (<0.5 µg/mL). Following two doses on day 0 and day 28 (2.5 µg of each conjugate per dose; total 10 µg), IgG geometric mean concentrations increased significantly (p < 0.001) by day 42, reaching 67.96 µg/mL (2a), 91.56 µg/mL (3a), 371.31 µg/mL (6), and 11.00 µg/mL (sonnei). Consistently, serum bactericidal activity (SBA) at day 42 increased 13-fold (2a), 34-fold (3a), 63-fold (6), and 224-fold (sonnei) relative to baseline (day 0). Conclusions: IVT Shigella-04 elicited robust serotype-specific humoral and functional immune responses in preclinical models, supporting its further development toward clinical evaluation. Full article

Given a finite set of interpolation data $\{(x_i,y_i)\in I\times \mathbb{R},i=0,1,\dots ,N\}$, $I=[x_0,x_N]$, we construct a class of nonlinear fractal interpolation functions whose graphs are realized as attractors of appropriately defined iterated function systems. In contrast to the classical framework based on uniform contraction mappings, the present approach is built upon an integral-type contraction condition, which extends the standard Banach setting to a more general and flexible context. By applying Branciari’s fixed point theorem, we prove the existence and uniqueness of continuous fractal interpolants associated with these systems. This generalized formulation contains the classical Barnsley fractal interpolation functions as a particular case, while allowing greater adaptability in the modeling of complex and irregular phenomena. As an application, the proposed methodology is implemented on real time-series data describing vaccination dynamics in four different countries, illustrating the effectiveness of the constructed fractal interpolation functions in approximating highly irregular real-world signals. Full article