Search Results (1,461)

This study examines the applicability of Plasma Transferred Arc (PTA) surface treatment as an advanced technique for the refurbishment of railway wheel treads. Conventional wheel reprofiling, typically performed on semi-automatic lathes, requires the removal of a minimum of 6 mm of material from the running surface, which accelerates rim thinning and ultimately necessitates wheel replacement. Moreover, the reprofiled surfaces are not subjected to any subsequent treatment aimed at enhancing their durability. To overcome these limitations, PTA cladding was selected due to its ability to generate surface layers with superior mechanical and tribological properties. In contrast to widely used diode laser technologies, PTA enables the deposition of alloying materials in powder form, ensuring a stable, controllable, and efficient cladding process. The resulting microstructure consists of a heat-affected zone, a transition zone, and a re-melted zone, each exhibiting significantly increased hardness relative to the untreated base material. The process facilitates the incorporation of metallic particles into the surface layer, promoting the formation of a dense, wear-resistant coating. These materials possess huge potential utility regarding the wear resistance reaching even ca 10% of the base material wear in the case of 505 PTA and over 20% in the case of the 15 E material. The findings indicate that PTA surface treatment has substantial potential to extend the operational lifespan of railway wheels by providing a highly durable and mechanically robust surface, thereby reducing maintenance frequency and the associated costs. Full article

Global warming is associated with an increased frequency and intensity of heat waves, which severely threaten crop production and sustainable agriculture. As sessile organisms, plants evolved complex heat stress memory mechanisms to cope with recurring heat waves. Heat shock transcription factors (HSFs) are at the core of plant heat stress responses and memory. They regulate basal thermotolerance, acquired thermotolerance, and the maintenance of acquired thermotolerance. These processes involve multiple mechanisms, including temperature perception, activation of heat shock protein expression, and integration of hormonal and epigenetic signals. Here, we review the pivotal role HSFs play in the formation of heat stress memory, their structural characteristics, functional differentiation, and signal perception and transcriptional regulatory mechanisms. We further discuss the functional conservation and the diversity of HSFs across multiple species—for instance, HSFA2 acts as a conserved regulator of heat stress memory in Arabidopsis, tomato, wheat, and barley—and outline future research directions, including the functional characterization of heat shock transcription factor (HSF) subfamilies, investigation of their roles under stress combination, and strategies to balance stress tolerance with growth and development. We hope that our review will provide a theoretical foundation for the genetic improvement of crop thermotolerance as well as contribute to efforts directed at ensuring food security in the face of climate change. Full article

Black ice poses a significant threat to drivers during winter due to its low visibility. Winter road maintenance personnel face continuous challenges in preventing its formation because of its high unpredictability. To address this issue, this paper proposes a practical strategy for effective winter road maintenance aimed at preventing black ice caused by freezing rain and frost. The strategy comprises three phases: black ice prediction, stakeholder notification, and anti-icing chemical application. The core of the strategy involves predicting black ice on rural highways that lack localized road weather sensors. Specifically, the prediction model relies exclusively on atmospheric data. The Extreme Gradient Boosting (XGBoost) algorithm was employed for prediction, achieving precision, recall, and F1 scores of 0.99 and outperforming Random Forest and Deep Neural Network models, which achieved F1 scores of 0.96 and 0.97, respectively. The XGBoost model’s hyperparameters were optimized using the DEPSO algorithm, improving its F1 score by approximately 0.03. Furthermore, a feature importance analysis was conducted to determine the relative contribution of various meteorological variables to black ice formation. To effectively disseminate predictive alerts to maintenance personnel and drivers, a smartphone-based system was developed. Finally, optimal spread rates for anti-icing chemicals, calibrated to pavement temperatures, are presented. The methodology proposed in this study can significantly enhance the efficiency of winter road maintenance on rural highways where localized road weather data are unavailable. Full article

The article proposes an integrated approach to prepare passenger cars for operation considering resource constraints and seasonal fluctuations in passenger traffic. The study is based on an analysis of the operational data on the volume of passenger car washing and water consumption during the period 2017–2024. The work uses methods of statistical analysis, regression modeling and numerical optimization. A close correlation between the number of passenger cars in the train and water consumption has been established, which allowed the formation of the corresponding regression dependencies and their integration into the structure of the optimization model. A mathematical model has been developed considering the time, resource and labor parameters of the process of preparing passenger vehicles and ensures a rational allocation of resources. The objective function combines time costs and the cost of processes using weighting factors, and the system of constraints considers technological, resource and organizational aspects. The model is adapted to variable operating conditions and provides for the possibility of scenario analysis. The results of the study showed that the use of automated passenger car wash complexes allows the reduction of water consumption by 10–12% and reduces the duration of washing by up to 20%. The use of the optimization model ensures a reduction of the total time for train preparation by 18–22% and a reduction in operating costs by 12–16%, which indicates an increase in the efficiency of resource use. Additionally, it was found that the introduction of automated technologies contributes to the reduction of the negative impact on the environment by reducing water consumption and reducing the level of wastewater pollution. The results obtained confirm the effectiveness of the proposed approach and the feasibility of its application in passenger cars’ maintenance systems. Full article

Background/Objectives: Alveolar ridge atrophy following tooth extraction complicates subsequent prosthetic rehabilitation and implant placement. This systematic review seeks to provide a comprehensive evaluation of existing evidence regarding the effectiveness of xenogeneic bone graft materials in socket preservation, particularly emphasizing their influence on the adjacent soft tissues. Methods: A systematic literature search was carried out across the CENTRAL, Embase, MEDLINE, PubMed, and Scopus databases. The search targeted randomized controlled trials (RCTs) published in English between January 2015 and September 2025 that examined xenogenic bone grafts used for ridge preservation in comparison either to spontaneous socket healing or to other types of grafting materials. The primary outcomes of interest were bone regeneration and alterations in soft tissues. Multiple independent reviewers performed study screening, data extraction, and risk of bias evaluation using the RoB 2 tool. Results: From 2242 initial records, 4 RCTs (138 patients) met the inclusion criteria. Studies compared xenografts (deproteinized bovine bone mineral with/without collagen), often combined with membranes, to unassisted healing. Augmentation techniques consistently showed a trend toward reduced horizontal and vertical ridge contraction compared to controls, though differences often lacked statistical significance (p > 0.05). Histological analysis revealed significantly less vital bone formation and residual graft particles in xenograft sites versus controls, suggesting volume maintenance is largely graft-dependent. Soft tissue contour changes were evaluated using 3D model scanning. Risk of bias varied, with concerns regarding randomization and reported outcomes in some studies. Conclusions: Xenogenic materials demonstrate comparable clinical effectiveness in preserving alveolar ridge dimensions, though radiographic volume stability may be partially attributed to the slow resorption of the graft material itself rather than new bone formation. Full article

Autonomous multi-drone navigation in unknown environments requires robust solutions for obstacle avoidance, formation cohesion, and inter-drone communication maintenance. We address these challenges using Riemannian Motion Policies (RMPs), a second-order dynamic framework for motion generation, within the RMPflow architecture. We design handcrafted and learned obstacle avoidance policies, including circle-based and LiDAR-based approaches, and introduce residual reinforcement learning as a novel mechanism for refining suboptimal handcrafted policies. To maintain reliable drone-to-drone communication, we propose a radio link quality policy that dynamically adapts the maximum permitted inter-drone distance based on signal strength, contrasting with a fixed worst-case baseline. These policies are composed within RMPflow into an efficient reactive motion planner and evaluated through 2D simulations across multiple configurations. Results show that residual learning substantially improves a suboptimal handcrafted policy, reducing collisions by 35.4% in drone formations and up to 74.7% for single drones. Notably, a policy trained without any initial obstacle avoidance capability failed to obtain such improvement, underscoring the importance of informed policy initialisation. The adaptive radio link policy achieved 20% to 25% shorter navigation time through randomly generated environments compared to a fixed maximum distance constraint, demonstrating the practical value of radio signal-aware formation control. Full article

Background/Objectives: Tooth impaction and transposition are developmental dental anomalies that pose significant challenges in orthodontics, oral surgery, and restorative dentistry. These anomalies disrupt normal occlusal development and complicate diagnosis and treatment planning because of altered anatomy, a higher risk of adjacent tooth resorption, associated pathology such as cyst formation, and biomechanical challenges during orthodontic intervention. Methods: Three paediatric patients with complex eruption disturbances, including impacted incisors, impacted primary molars, and transposed maxillary canines, were managed using individualised conservative orthodontic approaches. The cases involved obstruction by supernumerary teeth, ectopic eruption with space loss, and tooth transposition complicated by root dilaceration and malocclusion. Comprehensive clinical and radiographic assessment, including CBCT when indicated, guided treatment planning. Management strategies included staged surgical interventions, space maintenance using 2 × 4 appliances, bite opening, and controlled orthodontic traction with modified biomechanics to minimise the risk of root resorption and interference with adjacent teeth. Results: Favourable functional and aaesthetic outcomes were achieved in all cases, although certain limitations such as incomplete root development, residual spacing, localised restorative complications, and difficulty in correcting root angulation of dilacerated adjacent teeth were observed. Conclusions: Careful diagnosis using advanced imaging, combined with conservative orthodontic and surgical approaches, can achieve satisfactory functional and esthetic outcomes while minimising complications. Further longitudinal studies are recommended to evaluate long-term stability and optimise treatment protocols. Full article

ANCA-associated vasculitis (AAV) with kidney involvement represents small-vessel vasculitis, characterized by rapidly progressive glomerulonephritis and a high risk of end-stage kidney disease (ESKD) and increased mortality. AAV typically presents with multisystem involvement, with renal manifestations occurring more frequently in microscopic polyangiitis (MPA) (90–100%) and granulomatosis with polyangiitis (GPA) (50–80%). The classic clinical presentation includes acute kidney injury with hematuria and proteinuria, accompanied by ANCA positivity (MPO-ANCA or PR3-ANCA). Histologically, the predominant pattern is segmental necrotizing glomerulonephritis with crescent formation. Treatment consists of two phases: (a) induction of remission with a lower cumulative dose of glucocorticoids (according to the reduced-dose PEXIVAS regimen) in combination with rituximab or cyclophosphamide and (b) maintenance of remission with rituximab for 2–4 years. The C5a receptor inhibitor avacopan can be used as a steroid-sparing agent in patients with severe kidney involvement or at high risk of corticosteroid-related complications. Beyond the traditional markers of disease activity (hematuria, proteinuria, eGFR), novel biomarkers such as urinary soluble CD163, MCP-1, complement activation products (C5a, sC5b-9), and urinary Treg/Th17 profiles have demonstrated prognostic value. Early diagnosis and prompt initiation of immunosuppressive therapy significantly improve both kidney and overall survival, while prevention of relapses and long-term complications plays a key role in improving the long-term prognosis of patients with AAV. Full article

The safe and uninterrupted operation of the ship’s main engine is critical for maritime transportation. The shutdown mechanism, part of the main engine protection systems, prevents serious damage by automatically stopping the engine in critical situations such as low lubrication oil pressure, overspeed, high bearing temperature, and cooling system failures. However, identifying the faults that trigger the shutdown system and evaluating their risk levels is crucial for improving system reliability. In this study, shutdown events that may occur in a two-stroke low-speed marine diesel main engine were investigated using Fuzzy Fault Tree Analysis (FFTA). The shutdown event was defined as the peak event, and a total of 34 baseline events were modelled under five main branches: low lubrication oil pressure, overspeed, high thrust bearing temperature, abnormal jacket coolant inlet condition, and crankcase/cylinder oil mist formation. Fuzzy assessments based on expert opinions were defuzzified and converted into probability values and used in fault tree calculations. The results showed that the shutdown risk is largely affected by failures originating from the jacket coolant system and the lubrication oil system. Specifically, lubrication oil filter clogging and contamination/blockage in the coolant line were identified as the most critical risk factors. The findings significantly contribute to prioritizing maintenance and condition-monitoring activities aimed at improving the ship’s main engine reliability through a risk-based approach. Full article

Buds are continuously renewed sensory organs in which development, adult maintenance, and repair share overlapping molecular circuitry. During embryogenesis, WNT/β-catenin signaling promotes taste placode formation and placodal Shh expression, while SHH refines papilla spacing and restricts neighboring papilla formation. SOX2 functions as a taste-competence and progenitor maintenance factor. In adults, LGR5/LGR6–RSPO–WNT signaling sustains progenitor activity, and gustatory neurons are an important source of RSPO2; available genetic evidence is consistent with a neuron-derived contribution to the LGR5/LGR6 niche, and AAV-Cre-mediated neuron-specific ablation of Rspo2 in the petrosal ganglion led to near-complete loss of circumvallate taste buds. HH signaling from epithelial and neuronal sources further supports SOX2-dependent progenitor homeostasis. Lineage allocation is governed by transcriptional programs that include POU2F3/SKN-1a for sweet, umami, and bitter type II taste receptor cells, and ASCL1 with posterior-field NKX2-2 for type III presynaptic/sour cells. After denervation or irradiation, regeneration depends primarily on LGR5⁺/KRT14⁺ progenitors and may be supplemented, in specific injury contexts, by plasticity of a subset of K8-lineage taste receptor cells that acquire KRT14/SOX2/PCNA progenitor-like features. Key unresolved questions include the direct chromatin targets of taste lineage regulators (which remain to be defined by ChIP-seq in native taste progenitors), the identity of the type I cell selector, the contribution of dedifferentiation across injury models, and the degree to which mouse-derived networks are conserved in human taste biology. Full article

The bearded dragon (Pogona vitticeps) is the most commonly kept pet lizard and a promising model organism for studies of sex determination and gonadal development. Despite its potential, the morphological basis of gonadogenesis in this species remains poorly characterized. Here, we provide a comprehensive histological characterization of gonad development in P. vitticeps using serial paraffin and semi-thin sections, supplemented by morphometric analyses. Gonadal ridges first appeared at stage S28 as bilateral thickenings of the coelomic epithelium, coinciding with primordial germ cell colonization; by S28/29, a recognizable cortex and medulla were already present. The first morphological differences between male and female gonads appeared at S29/30. In differentiating testes, well-defined testis cords with a central lumen formed rapidly, while the cortex became thin and retained only scattered germ cells. Testicular development was characterized by rapid lumen formation within the testis cords, resulting in their early transformation into seminiferous tubules, followed by elongation and coiling of the tubules, maintenance of a stable tubule diameter, and a transient mitotic arrest of germ cells, with proliferation resuming at stage S36. In differentiating ovaries, the cortex remained thick and multilayered, with a progressive increase in germ cell number reflecting active oogonial proliferation. The ovarian medulla expanded substantially, and from S36 onward, lacunae developed within the medullary cords. No meiotic cells were observed at any examined stage. These results provide an essential morphological framework for future molecular and experimental studies of sex determination and gonadal differentiation in this species and in squamates more broadly. Full article

The humid coastal Lingnan region of South China, including the Chaoshan area of eastern Guangdong, is frequently exposed to disaster-prone weather conditions such as high humidity, typhoon-related winds, heavy rainfall, and salt-laden coastal air. These long-term environmental exposures may contribute to surface deterioration risks of architectural heritage. Located in Shantou, Yingchuan Shijia has shown five visible surface deterioration types—cracks, staining, saltpetering, plants, and spalling—under the combined influence of environmental exposure, material aging, previous disturbance, and insufficient maintenance. To address the limitations of manual inspection, this study explores a conservation-oriented intelligent workflow integrating YOLO-based detection, digital documentation, and screening-level conservation interpretation. Digital documentation used UAV imagery, mobile LiDAR scanning, measured drawings, and SketchUp-based three-dimensional modeling. The dataset was built in three stages: a 99-image preliminary dataset, where YOLOv8 showed only basic learning capability with low performance metrics, including Precision of 33.0 ± 3.0%, Recall of 28.0 ± 1.0%, mAP50 of 25.0 ± 1.0%, and mAP50-95 of 11.0 ± 1.0%; a 362-image non-augmented case-study dataset, where YOLOv8 still showed limited performance, with mAP50 of 20.0 ± 1.0% and mAP50-95 of 8.0 ± 1.0%; and a final YOLO-format case-study dataset of 2000 images after training-set-only augmentation using 11 geometric and photometric transformation methods. After augmentation, YOLOv8 mAP50 increased to 62.0 ± 2.0%. Under the same augmented-data condition, YOLOv13 showed Precision of 89.0 ± 1.0%, Recall of 77.0 ± 1.0%, mAP50 of 84.0 ± 1.0%, and mAP50-95 of 65.0 ± 1.0%, indicating relatively higher validation performance than YOLOv8. In the normalized confusion matrix, the background missed-detection values for cracks and saltpetering were 0.29 and 0.22, respectively, indicating that weak-feature and low-contrast deterioration types remained challenging. Based on YOLOv13, a mini program was developed to organize detection outputs and provide field-oriented preliminary conservation hints. Overall, this study provides a preliminary workflow linking digital collection, image-based deterioration detection, Grad-CAM visualization, and assisted field recording for the preventive conservation of Chaoshan overseas-Chinese residences in humid coastal heritage environments. Full article

Earthworms possess a highly developed innate immune system based on the coordinated activity of coelomocytes and humoral factors present in the coelomic fluid. These immune components play a central role in host defence against pathogens, maintenance of physiological homeostasis, and adaptation to environmental stressors. Coelomocytes exhibit remarkable functional and morphological diversity, including participation in phagocytosis, encapsulation, extracellular trap formation, cytotoxic responses, wound healing, and regulation of oxidative and osmotic stress. In addition, coelomic fluid contains numerous biologically active molecules, such as lysenin, coelomic cytolytic factor 1, perforin, serine proteases, lysozyme, antimicrobial peptides, and pattern recognition receptors, which contribute to cellular and humoral immune responses. Recent studies have demonstrated that earthworm coelomocytes are highly sensitive to environmental pollutants, including heavy metals, pesticides, nanomaterials, and microplastics, highlighting their importance in ecotoxicological research and soil biomonitoring. Furthermore, antifungal, antimicrobial, anti-inflammatory, antipyretic, and cytotoxic activities associated with coelomocytes and coelomic fluid suggest promising applications in agriculture, biotechnology, and pharmaceutical research. This review summarises current knowledge regarding the classification, characteristics, immune functions, toxicological responses, and applied significance of earthworm coelomocytes and coelomic fluid, with particular emphasis on their role in environmental monitoring and potential biomedical applications. Full article

Transcriptional bursting, the stochastic production of mRNA in episodic pulses, is a fundamental source of cell-to-cell heterogeneity. In pluripotent stem cells (PSCs), these bursting dynamics at core pluripotency loci are not just noise but critical determinants of identity maintenance and lineage commitment. This review synthesizes current quantitative frameworks for dissecting bursting kinetics and elaborates on the multilayered regulatory hierarchy that governs them, ranging from promoter-intrinsic features and 3D genome architecture to the formation of transcriptional condensates via liquid–liquid phase separation (LLPS). By integrating findings from genomic profiling and live-cell imaging, we highlight how the integrated action between super-enhancers and epigenetic states shapes the unique bursting dynamics in PSCs. Furthermore, we explore the functional consequences of these kinetics in pluripotency surveillance and cell fate decisions. Collectively, this review establishes a unified regulatory framework, providing novel insights for understanding stem cell heterogeneity and offering key insights for regenerative medicine. Full article

Deep wounds often lead to severe complications such as persistent infection, biofilm formation, and high patient morbidity. While skin injuries can usually be managed with functional dressings, wounds in deep layers without sufficient treatment may serve as primary entry points for bacterial infection, thereby posing a significant life-threatening risk to patients. With the rising prevalence of chronic diseases and an aging population, effective strategies for enhanced wound healing are still in high demand. In this study, an injectable and thermoresponsive hexamethylene diisocyanate–Pluronic F127 copolymer–hyaluronic acid composite hydrogel loaded with polyhexamethylene biguanide (PHMB) and epidermal growth factor (EGF), named PEHHPG, was developed for joint therapy of deep wounds. PEHHPG self-gels at 37 °C and stabilizes both agents in the gel matrix. Based on the results of microbial colony assay and analysis of fibroblast growth kinetics, PEHHPG with ≥200 ppm of PHMB and ≥0.15 μg/mL of EGF can eradicate bacteria and enhance cell proliferation in vitro, illustrating the functionalities of PEHHPG. Given the aforementioned effects, together with the recognized advantages of injectable hydrogels such as wound shape/depth adaptation, low adhesiveness, exudate absorptiveness, and moisture maintenance, the developed PEHHPG is anticipated to be a feasible dressing material for deep wound treatment after further in vivo examinations. Full article