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14 pages, 7789 KiB  
Article
Integrated Sampling Approaches Enhance Assessment of Saproxylic Beetle Biodiversity in a Mediterranean Forest Ecosystem (Sila National Park, Italy)
by Federica Mendicino, Francesco Carlomagno, Domenico Bonelli, Erica Di Biase, Federica Fumo and Teresa Bonacci
Insects 2025, 16(8), 812; https://doi.org/10.3390/insects16080812 (registering DOI) - 6 Aug 2025
Abstract
Saproxylic beetles are key bioindicators of forest ecosystem quality and play essential roles in deadwood decomposition and nutrient cycling. However, their populations are increasingly threatened by habitat fragmentation, deadwood removal, and climate-driven environmental changes. For this reason, an integrated sampling method can increase [...] Read more.
Saproxylic beetles are key bioindicators of forest ecosystem quality and play essential roles in deadwood decomposition and nutrient cycling. However, their populations are increasingly threatened by habitat fragmentation, deadwood removal, and climate-driven environmental changes. For this reason, an integrated sampling method can increase the detection of species with varying ecological traits. We evaluated the effectiveness of integrative sampling methodologies to assess saproxylic beetle diversity within Sila National Park, a Mediterranean forest ecosystem of high conservation value, specifically in two beech forests and four pine forests. The sampling methods tested included Pan Traps (PaTs), Malaise Traps (MTs), Pitfall Traps (PTs), Bait Bottle Traps (BBTs), and Visual Census (VC). All specimens were identified to the species level whenever possible, using specialized dichotomous keys and preserved in the Entomological Collection TB, Unical. Various trap types captured a different number of species: the PaT collected 32 species, followed by the PT with 24, the MT with 16, the VC with 7, and the BBT with 5 species. Interestingly, biodiversity analyses conducted using PAST software version 4.17 revealed that PaTs and MTs recorded the highest biodiversity indices. The GLMM analysis, performed using SPSS software 29.0.1.0, demonstrated that various traps attracted different species with different abundances. By combining multiple trapping techniques, we documented a more comprehensive community composition compared to single-method approaches. Moreover, PaTs, MTs, and PTs recorded 20%, 40%, and 33% of the Near Threatened species, respectively. We report new records for Sila National Park, including the LC species Pteryngium crenulatum (Curculionidae) and the NT species Grynocharis oblonga (Trogossitidae). For the first time in Calabria, the LC species Triplax rufipes (Erotylidae) and the NT species Oxypleurus nodieri (Cerambycidae) and Glischrochilus quadrisignatus (Nitidulidae) were collected. Our results emphasize the importance of method diversity in capturing species with distinct ecological requirements and highlight the relevance of saproxylic beetles as indicators of forest health. These findings support the adoption of multi-method sampling protocols in forest biodiversity monitoring and management programs, especially in biodiversity-rich and structurally heterogeneous landscapes. Full article
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27 pages, 14923 KiB  
Article
Multi-Sensor Flood Mapping in Urban and Agricultural Landscapes of the Netherlands Using SAR and Optical Data with Random Forest Classifier
by Omer Gokberk Narin, Aliihsan Sekertekin, Caglar Bayik, Filiz Bektas Balcik, Mahmut Arıkan, Fusun Balik Sanli and Saygin Abdikan
Remote Sens. 2025, 17(15), 2712; https://doi.org/10.3390/rs17152712 - 5 Aug 2025
Abstract
Floods stand as one of the most harmful natural disasters, which have become more dangerous because of climate change effects on urban structures and agricultural fields. This research presents a comprehensive flood mapping approach that combines multi-sensor satellite data with a machine learning [...] Read more.
Floods stand as one of the most harmful natural disasters, which have become more dangerous because of climate change effects on urban structures and agricultural fields. This research presents a comprehensive flood mapping approach that combines multi-sensor satellite data with a machine learning method to evaluate the July 2021 flood in the Netherlands. The research developed 25 different feature scenarios through the combination of Sentinel-1, Landsat-8, and Radarsat-2 imagery data by using backscattering coefficients together with optical Normalized Difference Water Index (NDWI) and Hue, Saturation, and Value (HSV) images and Synthetic Aperture Radar (SAR)-derived Grey Level Co-occurrence Matrix (GLCM) texture features. The Random Forest (RF) classifier was optimized before its application based on two different flood-prone regions, which included Zutphen’s urban area and Heijen’s agricultural land. Results demonstrated that the multi-sensor fusion scenarios (S18, S20, and S25) achieved the highest classification performance, with overall accuracy reaching 96.4% (Kappa = 0.906–0.949) in Zutphen and 87.5% (Kappa = 0.754–0.833) in Heijen. For the flood class F1 scores of all scenarios, they varied from 0.742 to 0.969 in Zutphen and from 0.626 to 0.969 in Heijen. Eventually, the addition of SAR texture metrics enhanced flood boundary identification throughout both urban and agricultural settings. Radarsat-2 provided limited benefits to the overall results, since Sentinel-1 and Landsat-8 data proved more effective despite being freely available. This study demonstrates that using SAR and optical features together with texture information creates a powerful and expandable flood mapping system, and RF classification performs well in diverse landscape settings. Full article
(This article belongs to the Special Issue Remote Sensing Applications in Flood Forecasting and Monitoring)
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14 pages, 3099 KiB  
Article
Identification of Keystone Plant Species for Avian Foraging and Nesting in Beijing’s Forest Ecosystems: Implications for Urban Forest Bird Conservation
by Lele Lin, Yongjian Zhao, Chao Yuan, Yushu Zhang, Siyu Qiu and Jixin Cao
Animals 2025, 15(15), 2271; https://doi.org/10.3390/ani15152271 - 4 Aug 2025
Viewed by 52
Abstract
Urban wildlife conservation is emerging as a critical component of sustainable city ecosystems. Rather than simply increasing tree abundance or species richness, conservation management should focus on key species. In this research, Xishan Forest Park in Beijing was chosen as a case study. [...] Read more.
Urban wildlife conservation is emerging as a critical component of sustainable city ecosystems. Rather than simply increasing tree abundance or species richness, conservation management should focus on key species. In this research, Xishan Forest Park in Beijing was chosen as a case study. Our aim was to identify keystone taxa critical for avian foraging and nesting during the breeding season. We performed a network analysis linking bird species, their diets, and nest plants. Dietary components were detected using DNA metabarcoding conducted with avian fecal samples. Nest plants were identified via transect surveys. Two indices of the network, degree and weighted mean degree, were calculated to evaluate the importance of the dietary and nest plant species. We identified 13 bird host species from 107 fecal samples and 14 bird species from 107 nest observations. Based on the degree indices, fruit trees Morus and Prunus were detected as key food sources, exhibiting both the highest degree (degree = 9, 9) and weighted mean degree (lnwMD = 5.21, 4.63). Robinia pseudoacacia provided predominant nesting sites, with a predominant degree of 7. A few taxa, such as Styphnolobium japonicum and Rhamnus parvifolia, served dual ecological significance as both essential food sources and nesting substrates. Scrublands, as a unique habitat type, provided nesting sites and food for small-bodied birds. Therefore, targeted management interventions are recommended to sustain or enhance these keystone resource species and to maintain the multi-layered vertical vegetation structure to preserve the diverse habitats of birds. Full article
(This article belongs to the Section Wildlife)
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15 pages, 428 KiB  
Article
Biodiversity Patterns and Community Construction in Subtropical Forests Driven by Species Phylogenetic Environments
by Pengcheng Liu, Jiejie Jiao, Chuping Wu, Weizhong Shao, Xuesong Liu and Liangjin Yao
Plants 2025, 14(15), 2397; https://doi.org/10.3390/plants14152397 - 2 Aug 2025
Viewed by 439
Abstract
To explore the characteristics of species diversity and phylogenetic diversity, as well as the dominant processes of community construction, in different forest types (deciduous broad-leaved forest, mixed coniferous and broad-leaved forest, and Chinese fir plantation) in subtropical regions, analyze the specific driving patterns [...] Read more.
To explore the characteristics of species diversity and phylogenetic diversity, as well as the dominant processes of community construction, in different forest types (deciduous broad-leaved forest, mixed coniferous and broad-leaved forest, and Chinese fir plantation) in subtropical regions, analyze the specific driving patterns of soil nutrients and other environmental factors on the formation of forest diversity in different forest types, and clarify the differences in response to environmental heterogeneity between natural forests and plantation forests. Based on 48 fixed monitoring plots of 50 m × 50 m in Shouchang Forest Farm, Jiande City, Zhejiang Province, woody plants with a diameter at breast height ≥5 cm were investigated. Species diversity indices (Margalef index, Shannon–Wiener index, Simpson index, and Pielou index), phylogenetic structure index (PD), and environmental factors were used to analyze the relationship between diversity characteristics and environmental factors through variance analysis, correlation analysis, and generalized linear models. Phylogenetic structural indices (NRI and NTI) were used, combined with a random zero model, to explore the mechanisms of community construction in different forest types. Research has found that (1) the deciduous broad-leaved forest had the highest species diversity (Margalef index of 4.121 ± 1.425) and phylogenetic diversity (PD index of 21.265 ± 7.796), significantly higher than the mixed coniferous and broad-leaved forest and the Chinese fir plantation (p < 0.05); (2) there is a significant positive correlation between species richness and phylogenetic diversity, with the best fit being AIC = 70.5636 and R2 = 0.9419 in broad-leaved forests; however, the contribution of evenness is limited; (3) the specific effects of soil factors on different forest types: available phosphorus (AP) is negatively correlated with the diversity of deciduous broad-leaved forests (p < 0.05), total phosphorus (TP) promotes the diversity of coniferous and broad-leaved mixed forests, while the diversity of Chinese fir plantations is significantly negatively correlated with total nitrogen (TN); (4) the phylogenetic structure of three different forest types shows a divergent pattern in deciduous broad-leaved forests, indicating that competition and exclusion dominate the construction of deciduous broad-leaved forests; the aggregation mode of Chinese fir plantation indicates that environmental filtering dominates the construction of Chinese fir plantation; the mixed coniferous and broad-leaved forest is a transitional model, indicating that the mixed coniferous and broad-leaved forest is influenced by both stochastic processes and ecological niche processes. In different forest types in subtropical regions, the species and phylogenetic diversity of broad-leaved forests is significantly higher than in other forest types. The impact of soil nutrients on the diversity of different forest types varies, and the characteristics of community construction in different forest types are also different. This indicates the importance of protecting the original vegetation and provides a scientific basis for improving the ecological function of artificial forest ecosystems through structural adjustment. The research results have important practical guidance value for sustainable forest management and biodiversity conservation in the region. Full article
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27 pages, 3387 KiB  
Article
Landscape Services from the Perspective of Experts and Their Use by the Local Community: A Comparative Study of Selected Landscape Types in a Region in Central Europe
by Piotr Krajewski, Marek Furmankiewicz, Marta Sylla, Iga Kołodyńska and Monika Lebiedzińska
Sustainability 2025, 17(15), 6998; https://doi.org/10.3390/su17156998 - 1 Aug 2025
Viewed by 169
Abstract
This study investigates the concept of landscape services (LS), which integrate environmental and sociocultural dimensions of sustainable development. Recognizing landscapes as essential to daily life and well-being, the research aims to support sustainable spatial planning by analyzing both their potential and their actual [...] Read more.
This study investigates the concept of landscape services (LS), which integrate environmental and sociocultural dimensions of sustainable development. Recognizing landscapes as essential to daily life and well-being, the research aims to support sustainable spatial planning by analyzing both their potential and their actual use. The study has three main objectives: (1) to assess the potential of 16 selected landscape types to provide six key LS through expert evaluation; (2) to determine actual LS usage patterns among the local community (residents); and (3) to identify agreements and discrepancies between expert assessments and resident use. The services analyzed include providing space for daily activities; regulating spatial structure through diversity and compositional richness; enhancing physical and mental health; enabling passive and active recreation; supporting personal fulfillment; and fostering social interaction. Expert-based surveys and participatory mapping with residents were used to assess the provision and use of LS. The results indicate consistent evaluations for forest and historical urban landscapes (high potential and use) and mining and transportation landscapes (low potential and use). However, significant differences emerged for mountain LS, rated highly by experts but used minimally by residents. These insights highlight the importance of aligning expert planning with community needs to promote sustainable land use policies and reduce spatial conflicts. Full article
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18 pages, 4332 KiB  
Article
Soils of the Settlements of the Yamal Region (Russia): Morphology, Diversity, and Their Environmental Role
by Evgeny Abakumov, Alexandr Pechkin, Sergey Kouzov and Anna Kravchuk
Appl. Sci. 2025, 15(15), 8569; https://doi.org/10.3390/app15158569 (registering DOI) - 1 Aug 2025
Viewed by 113
Abstract
The landscapes of the Arctic seem endless. But they are also subject to anthropogenic impact, especially in urbanized and industrial ecosystems. The population of the Arctic zone of Russia is extremely urbanized, and up to 84% of the population lives in cities and [...] Read more.
The landscapes of the Arctic seem endless. But they are also subject to anthropogenic impact, especially in urbanized and industrial ecosystems. The population of the Arctic zone of Russia is extremely urbanized, and up to 84% of the population lives in cities and industrial settlements. In this regard, we studied the background soils of forests and tundras and the soils of settlements. The main signs of the urbanogenic morphogenesis of soils associated with the transportation of material for urban construction are revealed. The peculiarities of soils of recreational, residential, and industrial zones of urbanized ecosystems are described. The questions of diversity and the classification of soils are discussed. The specificity of bulk soils used in the construction of industrial structures in the context of the initial stage of soil formation is considered. For the first time, soils and soil cover of settlements in the central and southern parts of the Yamal region are described in the context of traditional pedology. It is shown that the construction of new soils and grounds can lead to both decreases and increases in biodiversity, including the appearance of protected species. Surprisingly, the forms of urban soil formation in the Arctic are very diversified in terms of morphology, as well as in the ecological functions performed by soils. The urbanization of past decades has drastically changed the local soil cover. Full article
(This article belongs to the Section Environmental Sciences)
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21 pages, 1538 KiB  
Article
Soil Fungal Activity and Microbial Response to Wildfire in a Dry Tropical Forest of Northern Colombia
by Eliana Martínez Mera, Ana Carolina Torregroza-Espinosa, Ana Cristina De la Parra-Guerra, Marielena Durán-Castiblanco, William Zapata-Herazo, Juan Sebastián Rodríguez-Rebolledo, Fernán Zabala-Sierra and David Alejandro Blanco Alvarez
Diversity 2025, 17(8), 546; https://doi.org/10.3390/d17080546 - 1 Aug 2025
Viewed by 173
Abstract
Wildfires can significantly alter soil physicochemical conditions and microbial communities in forest ecosystems. This study aimed to characterize the culturable soil fungal community and evaluate biological activity in Banco Totumo Bijibana, a protected dry tropical forest in Atlántico, Colombia, affected by a wildfire [...] Read more.
Wildfires can significantly alter soil physicochemical conditions and microbial communities in forest ecosystems. This study aimed to characterize the culturable soil fungal community and evaluate biological activity in Banco Totumo Bijibana, a protected dry tropical forest in Atlántico, Colombia, affected by a wildfire in 2014. Twenty soil samples were collected for microbiological (10 cm depth) and physicochemical (30 cm) analysis. Basal respiration was measured using Stotzky’s method, nitrogen mineralization via Rawls’ method, and fungal diversity through culture-based identification and colony-forming unit (CFU) counts. Diversity was assessed using Simpson, Shannon–Weaver, and ACE indices. The soils presented low organic matter (0.70%) and nitrogen content (0.035%), with reduced biological activity as indicated by basal respiration (0.12 kg C ha−1 d−1) and mineralized nitrogen (5.61 kg ha−1). Four fungal morphotypes, likely from the genus Aspergillus, were identified. Simpson index indicated moderate dominance, while Shannon–Weaver values reflected low diversity. Correlation analysis showed Aspergillus-3 was positively associated with moisture, whereas Aspergillus-4 correlated negatively with pH and sand content. The species accumulation curve reached an asymptote, suggesting an adequate sampling effort. Although no control site was included, the findings provide a baseline characterization of post-fire soil microbial structure and function in a dry tropical ecosystem. Full article
(This article belongs to the Section Microbial Diversity and Culture Collections)
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31 pages, 2831 KiB  
Article
Structural Diversity and Biodiversity of Forest and Hedgerow in Areas Managed for Pheasant Shooting Across the UK
by Peter R. Long, Leo Petrokofsky, William J. Harvey, Paul Orsi, Matthew W. Jordon and Gillian Petrokofsky
Forests 2025, 16(8), 1249; https://doi.org/10.3390/f16081249 - 1 Aug 2025
Viewed by 201
Abstract
Management for pheasant shooting is a widespread land use in the UK, with potential implications for forest and hedgerow habitats. This study evaluates whether sites managed for pheasant shooting differ ecologically from similar sites not used for shooting. A systematic evidence evaluation of [...] Read more.
Management for pheasant shooting is a widespread land use in the UK, with potential implications for forest and hedgerow habitats. This study evaluates whether sites managed for pheasant shooting differ ecologically from similar sites not used for shooting. A systematic evidence evaluation of comparative studies was combined with a spatial analysis using remote sensing data (2010–2024). The literature review identified only 32 studies meeting strict criteria for comparability, revealing inconsistent and often weak evidence, with few studies reporting detailed forest management or statistically robust outcomes. While some studies noted increased or decreased biodiversity associated with pheasant shooting, the evidence base was generally of low quality. Remote sensing assessed forest structural and spectral diversity, intactness, and hedgerow density across 1131 pheasant-managed and 1131 matched control sites. Biodiversity data for birds, plants, and butterflies were sourced from GBIF records. Structural diversity and hedgerow density were significantly higher on pheasant-managed sites, while no significant differences were found in forest spectral diversity, intactness, or biodiversity indicators. Pheasant management may shape certain habitat features but has limited demonstrable effects on overall biodiversity. Further field-based, controlled studies are required to understand causal mechanisms and inform ecologically sustainable shooting practices. Full article
(This article belongs to the Special Issue Biodiversity and Ecosystem Functions in Forests)
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33 pages, 4670 KiB  
Article
Universal Prediction of CO2 Adsorption on Zeolites Using Machine Learning: A Comparative Analysis with Langmuir Isotherm Models
by Emrah Kirtil
ChemEngineering 2025, 9(4), 80; https://doi.org/10.3390/chemengineering9040080 - 28 Jul 2025
Viewed by 217
Abstract
The global atmospheric concentration of carbon dioxide (CO2) has exceeded 420 ppm. Adsorption-based carbon capture technologies, offer energy-efficient, sustainable solutions. Relying on classical adsorption models like Langmuir to predict CO2 uptake presents limitations due to the need for case-specific parameter [...] Read more.
The global atmospheric concentration of carbon dioxide (CO2) has exceeded 420 ppm. Adsorption-based carbon capture technologies, offer energy-efficient, sustainable solutions. Relying on classical adsorption models like Langmuir to predict CO2 uptake presents limitations due to the need for case-specific parameter fitting. To address this, the present study introduces a universal machine learning (ML) framework using multiple algorithms—Generalized Linear Model (GLM), Feed-forward Multilayer Perceptron (DL), Decision Tree (DT), Random Forest (RF), Support Vector Machine (SVM), and Gradient Boosted Trees (GBT)—to reliably predict CO2 adsorption capacities across diverse zeolite structures and conditions. By compiling over 5700 experimentally measured adsorption data points from 71 independent studies, this approach systematically incorporates critical factors including pore size, Si/Al ratio, cation type, temperature, and pressure. Rigorous Cross-Validation confirmed superior performance of the GBT model (R2 = 0.936, RMSE = 0.806 mmol/g), outperforming other ML models and providing comparable performance with classical Langmuir model predictions without separate parameter calibration. Feature importance analysis identified pressure, Si/Al ratio, and cation type as dominant influences on adsorption performance. Overall, this ML-driven methodology demonstrates substantial promise for accelerating material discovery, optimization, and practical deployment of zeolite-based CO2 capture technologies. Full article
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17 pages, 1268 KiB  
Article
Community Composition and Diversity of β-Glucosidase Genes in Soils by Amplicon Sequence Variant Analysis
by Luis Jimenez
Genes 2025, 16(8), 900; https://doi.org/10.3390/genes16080900 - 28 Jul 2025
Viewed by 181
Abstract
Cellulose, the most abundant organic polymer in soil, is degraded by the action of microbial communities. Cellulolytic taxa are widespread in soils, enhancing the biodegradation of cellulose by the synergistic action of different cellulase enzymes. β-glucosidases are the last enzymes responsible for the [...] Read more.
Cellulose, the most abundant organic polymer in soil, is degraded by the action of microbial communities. Cellulolytic taxa are widespread in soils, enhancing the biodegradation of cellulose by the synergistic action of different cellulase enzymes. β-glucosidases are the last enzymes responsible for the degradation of cellulose by producing glucose from the conversion of the disaccharide cellobiose. Different soils from the states of Delaware, Maryland, New Jersey, and New York were analyzed by direct DNA extraction, PCR analysis, and next generation sequencing of amplicon sequences coding for β-glucosidase genes. To determine the community structure and diversity of microorganisms carrying β-glucosidase genes, amplicon sequence variant analysis was performed. Results showed that the majority of β-glucosidase genes did not match any known phylum or genera with an average of 84% of sequences identified as unclassified. The forest soil sample from New York showed the highest value with 95.62%. When identification was possible, the bacterial phyla Pseudomonadota, Actinomycetota, and Chloroflexota were found to be dominant microorganisms with β-glucosidase genes in soils. The Delaware soil showed the highest diversity with phyla and genera showing the presence of β-glucosidase gene sequences in bacteria, fungi, and plants. However, the Chloroflexota genus Kallotanue was detected in 3 out of the 4 soil locations. When phylogenetic analysis of unclassified β-glucosidase genes was completed, most sequences aligned with the Chloroflexota genus Kallotenue and the Pseudomonadota species Sphingomonas paucimobilis. Since most sequences did not match known phyla, there is tremendous potential to discover new enzymes for possible biotechnological and pharmaceutical applications. Full article
(This article belongs to the Section Microbial Genetics and Genomics)
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13 pages, 1075 KiB  
Article
Response of Typical Artificial Forest Soil Microbial Community to Revegetation in the Loess Plateau, China
by Xiaohua Liu, Tianxing Wei, Dehui Fan, Huaxing Bi and Qingke Zhu
Agronomy 2025, 15(8), 1821; https://doi.org/10.3390/agronomy15081821 - 28 Jul 2025
Viewed by 212
Abstract
This study aims to analyze the differences in soil bacterial community structure under different vegetation restoration types, and to explore the role of microorganisms in the process of vegetation restoration on the soil ecosystem of the Grain for Green area in the Loess [...] Read more.
This study aims to analyze the differences in soil bacterial community structure under different vegetation restoration types, and to explore the role of microorganisms in the process of vegetation restoration on the soil ecosystem of the Grain for Green area in the Loess Plateau. High-throughput sequencing technology was used to analyze the alpha diversity of soil bacteria, community structure characteristics, and the correlation between soil environmental factors and bacterial communities in different artificial Hippophae rhamnoides forests. Soil microbial C and N show a decreasing trend with an increase in the 0–100 cm soil layers. The results indicated that the bacterial communities comprised 24 phyla, 55 classes, 110 orders, 206 families, 348 genera, 680 species, and 1989 OTUs. Additionally, the richness indices and diversity indices of the bacterial community in arbor shrub mixed forest are higher than those in shrub pure forest, and the indices of shrub forest on sunny slope are higher than those on shady slope. Across all samples, the dominant groups were Actinobacteria (37.27% on average), followed by Proteobacteria (23.91%), Acidobacteria (12.75%), and Chloroflexi (12.27%). Soil nutrient supply, such as TOC, TN, AN, AP, and AK, had crucial roles in shaping the composition and diversity of the bacterial communities. The findings reveal that vegetation restoration significantly affected soil bacterial community richness and diversity. Furthermore, based on the results, our data provide a starting point for establishing soil bacterial databases in the Loess Plateau, as well as for the plants associated with the vegetation restoration. Full article
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24 pages, 5785 KiB  
Article
Phylogenetic Reassessment of Murinae Inferred from the Mitogenome of the Monotypic Genus Dacnomys Endemic to Southeast Asia: New Insights into Genetic Diversity Erosion
by Zhongsong Wang, Di Zhao, Wenyu Song and Wenge Dong
Biology 2025, 14(8), 948; https://doi.org/10.3390/biology14080948 - 28 Jul 2025
Viewed by 325
Abstract
The Millard’s rat (Dacnomys millardi), a threatened murid endemic to Southeast Asian montane rainforests and the sole member of its monotypic genus, faces escalating endangered risks as a Near Threatened species in China’s Biodiversity Red List. This ecologically specialized rodent exhibits [...] Read more.
The Millard’s rat (Dacnomys millardi), a threatened murid endemic to Southeast Asian montane rainforests and the sole member of its monotypic genus, faces escalating endangered risks as a Near Threatened species in China’s Biodiversity Red List. This ecologically specialized rodent exhibits diagnostic morphological adaptations—hypertrophied upper molars and cryptic pelage—that underpin niche differentiation in undisturbed tropical/subtropical forests. Despite its evolutionary distinctiveness, the conservation prioritization given to Dacnomys is hindered due to a deficiency of data and unresolved phylogenetic relationships. Here, we integrated morphological analyses with the first complete mitogenome (16,289 bp in size; no structural rearrangements) of D. millardi to validate its phylogenetic placement within the subfamily Murinae and provide novel insights into genetic diversity erosion. Bayesian and maximum likelihood phylogenies robustly supported Dacnomys as sister to Leopoldamys (PP = 1.0; BS = 100%), with an early Pliocene divergence (~4.8 Mya, 95% HPD: 3.65–5.47 Mya). Additionally, based on its basal phylogenetic position within Murinae, we propose reclassifying Micromys from Rattini to the tribe Micromyini. Codon usage bias analyses revealed pervasive purifying selection (Ka/Ks < 1), constraining mitogenome evolution. Genetic diversity analyses showed low genetic variation (CYTB: π = 0.0135 ± 0.0023; COX1: π = 0.0101 ± 0.0025) in fragmented populations. We propose three new insights into this genetic diversity erosion. (1) Evolutionary constraints: genome-wide evolutionary conservation and shallow evolutionary history (~4.8 Mya) limited mutation accumulation. (2) Anthropogenic pressures: deforestation-driven fragmentation of habitats (>20,000 km2/year loss since 2000) has reduced effective population size, exacerbating genetic drift. (3) Ecological specialization: long-term adaptation to stable niches favored genomic optimization over adaptive flexibility. These findings necessitate suitable conservation action by enforcing protection of core habitats to prevent deforestation-driven population collapses and advocating IUCN reclassification of D. millardi from Data Deficient to Near Threatened. Full article
(This article belongs to the Section Genetics and Genomics)
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12 pages, 9023 KiB  
Article
The Impact of Vegetation Structure on Shaping Urban Avian Communities in Chaoyang District Beijing, China
by Anees Ur Rahman, Kamran Ullah, Shumaila Batool, Rashid Rasool Rabbani Ismaili and Liping Yan
Animals 2025, 15(15), 2214; https://doi.org/10.3390/ani15152214 - 28 Jul 2025
Viewed by 275
Abstract
This study examines the impact of vegetation structure on bird species richness and diversity across four urban parks in Chaoyang District, Beijing. Throughout the year, using the Point Count Method (PCM), a total of 68 bird species and 4279 individual observations were recorded, [...] Read more.
This study examines the impact of vegetation structure on bird species richness and diversity across four urban parks in Chaoyang District, Beijing. Throughout the year, using the Point Count Method (PCM), a total of 68 bird species and 4279 individual observations were recorded, with surveys conducted across all four seasons to capture seasonal variations. The parks with more complex vegetation, such as those with a higher tree canopy cover of species like poplars, ginkgo, and Chinese pines, exhibited higher bird species richness. For example, Olympic Forest Park, with its dense vegetation structure, hosted 42 species, whereas parks with less diverse vegetation supported fewer species. An analysis using PERMANOVA revealed that bird communities in the four parks were significantly different from each other (F = 2.76, p = 0.04075), and every comparison between parks showed significant differences as well (p < 0.001). Variations in the arrangement and level of disturbance within different plant communities likely cause such differences. Principal component analysis (PCA) identified tree canopy cover and shrub density as key drivers of bird diversity. These findings underscore the importance of preserving urban green spaces, particularly those with a diverse range of native tree species, to conserve biodiversity and mitigate the adverse effects of urbanisation. Effective vegetation management strategies can enhance avian habitats and provide ecological and cultural benefits in urban environments. Full article
(This article belongs to the Section Birds)
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22 pages, 1781 KiB  
Article
Analyzing Heart Rate Variability for COVID-19 ICU Mortality Prediction Using Continuous Signal Processing Techniques
by Guilherme David, André Lourenço, Cristiana P. Von Rekowski, Iola Pinto, Cecília R. C. Calado and Luís Bento
J. Clin. Med. 2025, 14(15), 5312; https://doi.org/10.3390/jcm14155312 - 28 Jul 2025
Viewed by 261
Abstract
Background/Objectives: Heart rate variability (HRV) has been widely investigated as a predictor of disease and mortality across diverse patient populations; however, there remains no consensus on the optimal set or combination of time and frequency domain nor on nonlinear features for reliable prediction [...] Read more.
Background/Objectives: Heart rate variability (HRV) has been widely investigated as a predictor of disease and mortality across diverse patient populations; however, there remains no consensus on the optimal set or combination of time and frequency domain nor on nonlinear features for reliable prediction across clinical contexts. Given the relevance of the COVID-19 pandemic and the unique clinical profiles of these patients, this retrospective observational study explored the potential of HRV analysis for early prediction of in-hospital mortality using ECG signals recorded during the initial moments of ICU admission in COVID-19 patients. Methods: HRV indices were extracted from four ECG leads (I, II, III, and aVF) using sliding windows of 2, 5, and 7 min across observation intervals of 15, 30, and 60 min. The raw data posed significant challenges in terms of structure, synchronization, and signal quality; thus, from an original set of 381 records from 321 patients, after data pre-processing steps, a final dataset of 82 patients was selected for analysis. To manage data complexity and evaluate predictive performance, two feature selection methods, four feature reduction techniques, and five classification models were applied to identify the optimal approach. Results: Among the feature aggregation methods, compiling feature means across patient windows (Method D) yielded the best results, particularly for longer observation intervals (e.g., using LDA, the best AUC of 0.82±0.13 was obtained with Method D versus 0.63±0.09 with Method C using 5 min windows). Linear Discriminant Analysis (LDA) was the most consistent classification algorithm, demonstrating robust performance across various time windows and further improvement with dimensionality reduction. Although Gradient Boosting and Random Forest also achieved high AUCs and F1-scores, their performance outcomes varied across time intervals. Conclusions: These findings support the feasibility and clinical relevance of using short-term HRV as a noninvasive, data-driven tool for early risk stratification in critical care, potentially guiding timely therapeutic decisions in high-risk ICU patients and thereby reducing in-hospital mortality. Full article
(This article belongs to the Section Cardiology)
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25 pages, 5461 KiB  
Article
Spaceborne LiDAR Reveals Anthropogenic and Biophysical Drivers Shaping the Spatial Distribution of Forest Aboveground Biomass in Eastern Himalayas
by Abhilash Dutta Roy, Abraham Ranglong, Sandeep Timilsina, Sumit Kumar Das, Michael S. Watt, Sergio de-Miguel, Sourabh Deb, Uttam Kumar Sahoo and Midhun Mohan
Land 2025, 14(8), 1540; https://doi.org/10.3390/land14081540 - 27 Jul 2025
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Abstract
The distribution of forest aboveground biomass density (AGBD) is a key indicator of carbon stock and ecosystem health in the Eastern Himalayas, which represents a global biodiversity hotspot that sustains diverse forest types across an elevation gradient from lowland rainforests to alpine meadows [...] Read more.
The distribution of forest aboveground biomass density (AGBD) is a key indicator of carbon stock and ecosystem health in the Eastern Himalayas, which represents a global biodiversity hotspot that sustains diverse forest types across an elevation gradient from lowland rainforests to alpine meadows and contributes to the livelihoods of more than 200 distinct indigenous communities. This study aimed to identify the key factors influencing forest AGBD across this region by analyzing the underlying biophysical and anthropogenic drivers through machine learning (random forest). We processed AGBD data from the Global Ecosystem Dynamics Investigation (GEDI) spaceborne LiDAR and applied filtering to retain 30,257 high-quality footprints across ten ecoregions. We then analyzed the relationship between AGBD and 17 climatic, topographic, soil, and anthropogenic variables using random forest regression models. The results revealed significant spatial variability in AGBD (149.6 ± 79.5 Mg ha−1) across the region. State-wise, Sikkim recorded the highest mean AGBD (218 Mg ha−1) and Manipur the lowest (102.8 Mg ha−1). Within individual ecoregions, the Himalayan subtropical pine forests exhibited the highest mean AGBD (245.5 Mg ha−1). Topographic factors, particularly elevation and latitude, were strong determinants of biomass distribution, with AGBD increasing up to elevations of 2000 m before declining. Protected areas (PAs) consistently showed higher AGBD than unprotected forests for all ecoregions, while proximity to urban and agricultural areas resulted in lower AGBD, pointing towards negative anthropogenic impacts. Our full model explained 41% of AGBD variance across the Eastern Himalayas, with better performance in individual ecoregions like the Northeast India-Myanmar pine forests (R2 = 0.59). While limited by the absence of regionally explicit stand-level forest structure data (age, stand density, species composition), our results provide valuable evidence for conservation policy development, including expansion of PAs, compensating avoided deforestation and modifications in shifting cultivation. Future research should integrate field measurements with remote sensing and use high-resolution LiDAR with locally derived allometric models to enhance biomass estimation and GEDI data validation. Full article
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