Search Results (143)

Forage crops play a vital role in ensuring livestock productivity and food security in Northern Kazakhstan, a region characterized by highly variable weather conditions. However, traditional methods for assessing crop maturity remain time-consuming and labor-intensive, underscoring the need for automated monitoring solutions. Recent advances in remote sensing and artificial intelligence (AI) offer new opportunities to address this challenge. In this study, unmanned aerial vehicle (UAV)-based multispectral imaging was used to monitor the development of forage crops—pea, sudangrass, common vetch, oat—and their mixtures under field conditions in Northern Kazakhstan. A multispectral dataset consisting of five spectral bands was collected and processed to generate vegetation indices. Using a ResNet-based neural network model, the study achieved a high predictive accuracy ($R^2$ = 0.985) for estimating the continuous maturity index. The trained model was further integrated into a web-based platform to enable real-time visualization and analysis, providing a practical tool for automated crop maturity assessment and long-term agricultural monitoring. Full article

The Ddw1 dwarfing gene is pivotal for reducing plant height and improving lodging resistance in triticale, but its pleiotropic effects on yield components and heading date pose significant challenges for breeders. This study identifies the Growth-Regulating Factor 9-6A (GRF9-6A) gene that compensates for these drawbacks. Field trials across locations (Moscow, Krasnodar) and years (2018, 2019) confirmed the effect of Ddw1, reducing plant height by 26.1–30.1 cm (31.8–32.5%) and thousand-kernel weight (TKW) by 6.0–6.3 g (11.8–15.7%) while increasing the harvest index by up to 4.8 percentage points in three out of four environments, with its effect nullified under the heat stress of Krasnodar 2019. In a Ddw1 background, the GRF9-6Ab allele distinguished by a 12 bp deletion in its promoter compensated for yield losses by increasing grain weight per main spike by up to 0.34 g (19.2%) via boosts in grain number per spike (up to 11.6%) and TKW (up to 11.5%). This combination also elevated the harvest index by up to 4.0 pp and, critically, provided a key agronomic advantage by accelerating heading by 6.3–6.5 days (10.0–10.2%), thereby mitigating the Ddw1-associated developmental delay. Our results demonstrate that pyramiding Ddw1 with the GRF9-6Ab allele the development of semi-dwarf, high-yielding, and early-maturing triticale cultivars for food and forage. This breeding strategy, facilitated by a newly developed diagnostic marker for GRF9-6Ab, is particularly advantageous for cultivation in environments similar to the Central Non-Chernozem region. Full article

The global feed gap, driven by seasonal shortages and climate change, highlights the need for novel forage resources. Vicia faba (Faba bean) produces substantial above-ground biomass as residue after fresh pod harvest, which remains underutilized. This study comprehensively evaluated the forage potential of faba bean leaves and stems across three growth stages: flowering (S1), pod development (S2), and ripening (S3). Dry matter content peaked at S2 in both tissues, while crude protein and fat content were highest at S1; carbohydrate levels increased progressively with maturation. Significant mineral concentrations, particularly K, Ca, and Mg, were detected, with leaves at S2 showing higher ash (i.e., mineral) content. Bioactive compounds (L-dopa, flavonols, total phenolics, and flavonoids) and antioxidant activities were most abundant at S1, with strong positive correlations between phenolics and antioxidant activities. Overall, faba bean residues offer proximate nutritional profiles comparable to traditional forages such as alfalfa and clover, while providing superior antioxidant potential. Their incorporation into animal feed systems before S3 could help mitigate seasonal forage shortages and enhance the nutritional quality of livestock diets. Full article

The present experiment aimed to investigate the effects of harvest stages on the fermentation quality and nutritional value of sorghum stalk silage. Sorghum stalks were harvested at the three stages (milk, dough, and ripe), chopped, and ensiled for 60 d. Each treatment had five replicates, and the silages were evaluated for fermentation quality, nutritional composition, in vitro rumen fermentation characteristics, and bacterial community profiles. The results showed that the crude protein, neutral detergent fiber, and acid detergent fiber contents decreased significantly with harvest maturity (p < 0.05). Consequently, silage from the ripe stage possessed the highest dry matter, relative feed value, and total digestible nutrients (p < 0.05). In vitro rumen fermentation indicated that the ripe stage silage exhibited the greatest gas production, and the lowest concentrations of ruminal ammonia–nitrogen (p < 0.05). Microbial analysis revealed a shift from dominant epiphytic Proteobacteria to fermentative Firmicutes post-ensiling, with the ripe stage community co-dominated by Lactobacillus and Leuconostoc, in contrast to the milk stage’s enrichment with Klebsiella. In conclusion, harvesting sorghum at the ripe stage is the optimal strategy as it establishes a beneficial microbial community, resulting in silage with superior nutritional value and rumen fermentation efficiency. Full article

Three purple prairie clover (PPC; Dalea purpurea Vent.) varieties, namely Common seed (CS), AC Lamour (ACL) and Bismarck (BIS), were established in plots of irrigated land (rain-fed plus irrigation, Lethbridge, AB) and dryland (rain-fed only, Swift Current, SK) to assess its agronomic characteristics and nutritive value under different ecoclimate and growing conditions in Western Canada. Each seed source was replicated in four test plots arranged as a randomized complete block design at each experimental site. Forage mass on dry matter (DM) basis, canopy height, proportions of leaf and stem and nutritive value were determined at vegetative (VEG), full flower (FF) and late flower (LF) phenological stages. The forage masses of the three PPC varieties were similar (p < 0.05) at each phenological stage with the mean values for VFG, FF and LF being 4739, 4988 and 6753 kg DM/ha under the Lethbridge irrigated conditions, and 1423, 2014 and 2297 kg DM/ha under the Swift Current dryland conditions. The forage mass was higher (p < 0.001) under Lethbridge irrigation than under Swift Current dryland conditions and increased (p < 0.05) with maturity. The three varieties had similar concentrations of organic matter (OM), neutral detergent fibre (NDF), acid detergent fibre (ADF) and crude protein (CP) and in vitro DM digestibility (DMD) at each phenological stage, but CP concentration and in vitro DMD decreased (p < 0.001) whilst NDF and ADF concentration increased (p < 0.001) with maturity. Purple prairie clover grown at Lethbridge irrigated land had higher (p < 0.001) DMD, OM and CP, but lower (p < 0.001) NDF, ADF and condensed tannin concentrations than that grown at Swift Current dryland conditions. These results indicate that PPC has great potential as an alternative legume forage for the cattle industry. Full article

The consumption of forages high in slow-degradable carbohydrates by dairy cattle leads to greater ruminal acetate production, which benefits milk fat content. Although tropical grasses are typically rich in fibrous materials, the milk fat content of dairy cows in Southeast Asia is low. Here, we investigate the effects of the species and harvest time of three common tropical grasses (Guinea, King, and Mulato II) harvested at three instances (early, normal, late) on in sacco degradation kinetics and in vitro gas production (IVGP) characteristics. Grass samples were subjected to (1) chemical analysis, (2) a fully automated in vitro gas recording system using rumen fluid to measure fermentation characteristics over 72 h, and (3) in sacco degradation using the nylon bag technique, employing seven incubation times up to 336 h. Forage quality decreased with maturity, as reflected in changes to digestibility and fiber content. Overall, early harvested grasses yielded the highest total gas production (311 ± 12.5 mL/g OM) followed by normal (300 ± 45.7 mL/g OM) and late (273 ± 19.5 mL/g OM) harvested grasses. The in vitro fermentable fraction (A1 + A2) was the highest for early harvested grasses, with the A2 parameter, relevant for milk fat content, being the highest for Guinea (81.6% A2/(A1 + A2)) and the lowest for King grass (71.0% A2/(A1 + A2)). Consequently, early harvested Guinea had the longest incubation times (10.5 h) and lowest fermentation rates (Rmax2 = 12.8 mL/g OM/h). Regression analysis showed relationships between NDF content and degradability. Harvesting tropical grass earlier than customarily practiced enhanced forage quality and ruminal degradability. Of the three grasses studied, each at three levels of maturity, early harvested Guinea grass was the most promising candidate for improving milk fat content in Southeast Asian dairy cows. This grass showed a high fermentable OM content, with a large proportion of slow-degradable carbohydrates. Full article

Malaria and other haemosporidian parasites are common in reptiles. During baseline health surveys of sea turtles in Western Australia (WA), haemosporidian parasites were detected in flatback (Natator depressus) and green (Chelonia mydas) turtle erythrocytes during routine blood film examination. 130 blood samples were screened via polymerase chain reaction (PCR), including 105 N. depressus, 20 C. mydas, and 5 olive ridley turtles (Lepidochelys olivacea). A novel Haemocystidium sp. was identified, detected exclusively in foraging turtles and not in nesting turtles. The combined prevalence by microscopic and molecular methods was 16.9% (22/130), primarily affecting immature C. mydas (77.3%; 17/22). Mature N. depressus were also affected (22.7%; 5/22). DNA sequencing of a partial fragment of the mitochondrial cytochrome b (cytb) gene together with phylogenetic analysis identified two different Haemocystidium sp. genotypes, A and B, with genotype A being most prevalent. The phylogenetic analysis showed close genetic relationships to Haemocystidium sp. in freshwater and terrestrial turtles, suggesting a shared evolutionary lineage despite ecological differences. Preliminary analysis indicates that this parasite is incidental, as no association between health and parasite presence or grade was detected. This study provides the first formal detection of haemosporidian parasites in sea turtles, contributing essential baseline data while highlighting their evolutionary significance and host–parasite ecological relationships. Full article

Rhamdiopsis krugi is a highly specialized troglobitic (exclusively subterranean) catfish endemic to the phreatic water bodies of twelve caves located within two separated metasedimentary basins in the region of Chapada Diamantina, Bahia state, Brazil. This species is included in the List of Endangered Fauna of Brazil, under the Vulnerable category—VU. In general, troglobites have different strategies for searching for food and reproductive partners, as well as unique behaviors. Knowledge of the reproductive periods, as well as its feeding habits, provides fundamental data for effective protection and species conservation. Biological aspects related to feeding habits and reproduction of R. krugi were addressed across six annual cycles, considering both dry and rainy seasons. For this, stomach content analysis, using the frequency of occurrence and volumetric index methods, as well as observation of the sex ratio and stage of maturation of the gonads were carried out for 148 individuals of R. krugi sampled in eight caves in Chapada Diamantina. Stomach volumes correlated with reproduction aspects across the dry and rainy seasons. These populations showed opportunistic carnivorous feeding habits, consuming both autochthonous and allochthonous items, with a preference for foraging in submerged guano deposits, which demonstrates the catfish’s strong dependence on bats. Regarding sex ratios, there was no marked seasonality; however, in rainy seasons, there was a higher proportion of maturing females, showing a reproductive tendency. During these periods, there was also a significantly higher number of stomachs with contents, showing seasonality in the diet. Specialized diet and dependence on rainy periods, especially in diet, corroborate the fragility of R. krugi, especially considering the changes in rainfall regimes in Brazil, with dry seasons exceeding eight months per year in the last ten years. Full article

This study describes the first successful rearing of capelin from hatch to adulthood in a laboratory setting using intensive culture methods. Over the span of about two years, the capelin were reared in aquaculture tanks under a constant temperature of 7 °C. The capelin demonstrated a robust linear growth during their first year of life, with a mean length increment of 0.36 mm per day. Due to their accelerated growth, some of the capelin became sexually mature as early as one year post-hatch. The first year was characterized by a rapid increase in condition factor (CF) while the second year showed a plateau. The von Bertalanffy growth equation effectively described the two-year growth of the cultivated capelin, predicting an asymptotic length (L∞) of 18.4 cm, similar to the 18.6 cm median L∞ of wild Icelandic capelin (1981–2018 cohorts). The cultivated capelin were projected to reach this length in 2.6 years, compared to about 6 years for wild capelin. This study provides new insights into the growth dynamics of capelin and although the species is sensitive to handling, it demonstrates that intensive culture methods can be used to investigate biological aspects of this important forage species. Full article

Brachiaria forages are known to be drought-tolerant as mature plants, but no information about drought tolerance at the seed germination stage is currently available. This study aimed to determine the impacts of different temperature and moisture conditions on the seed germination characteristics of five Brachiaria genotypes. Brachiaria seeds were germinated under constant temperatures of 5 °C–45 °C at increments of 5 °C. Within each temperature treatment, five osmotic treatments (0 MPa, −0.1 MPa, −0.3 MPa, −0.5 MPa, and −0.7 MPa) were applied, and germination was recorded daily for 20 days. The results showed that seed germination in all Brachiaria species was significantly negatively impacted (p < 0.05) by osmotic stress as well as by high and low temperatures. For all species, germination only occurred between 15 and 40 °C. Under optimum moisture conditions (0 MPa), the optimum germination temperatures for B. humidicola were 15 to 35 °C, for B. brizantha and B. nigropedata, they were 15 to 20 °C, for B. decumbens, they were 15 to 25 °C, and for the hybrid Brachiaria species, the optimum germination temperature was only 20 °C. In all species, seed germination decreased as moisture conditions became more limiting. Only B. humidicola germinated optimally at a high temperature (35 °C). At these temperatures, the species had more than 82% germination when moisture was not a limiting factor (0 MPa), but at low osmotic stress conditions (−0.1 MPa) at 30 °C, the germination of this species decreased to 67%. In conclusion, the results from this study indicate that the seed germination and early seedling establishment stages of Brachiaria grasses are only moderately tolerant to drought stress. Further work on early seedling responses to temperature and moisture stresses is needed to quantify early seedling responses to these stresses and to develop more detailed planting time guidelines for farmers. Full article

This study examines the impact of line spacing (X: 24 m, Y: 9 m, Z: 6.5 m) and orientation to tree lines on the growth, yield, and quality of red clover (Trifolium pratense L.) in a temperate, irrigated agroforestry system (2 ha) in Szarvas, Hungary. Three sampling locations were distinguished between the east and west oriented tree lines: the north (N) side, middle (M) strip, and south (S) side of the tree lines. The highest red clovers were observed in the 6.5 m spacing (mean height 69.3 ± 7.2 cm), although yields were similar across 24 m, 9 m, and 6.5 m spacings (2.9 t ha⁻¹, 2.3 t ha⁻¹, and 2.7 t ha⁻¹ dry matter, respectively). Orientation significantly influenced all forage quality parameters, with the north side showing earlier developmental stages and higher proportions of immature flowers (41–59%). Managing the spatial arrangement of red clover in agroforestry systems can help optimize forage quality by mitigating variations in plant maturity. Full article

Stockpiling forage (i.e., deferring grazing) is one way to provide forage for livestock during intervals of low pasture growth, but there are trade-offs as nutrient content declines with increasing forage maturity. Phosphorous (P) concentration, crude protein (CP) content and organic matter digestibility (OMD) were evaluated in two C3 and four C4 grasses native to the South American Campos grasslands. These were: Bromus auleticus (BROAUL) and Nasella neessiana (NASNEE) as C3 grasses and Andropogon lateralis (ANDLAT), Mnesithea selloana (MNESEL), Paspalum dilatatum (PASDIL), and Paspalum notatum (PASNOT) as C4 grasses. The grasses were grown in pots during five stockpiling periods (450, 900, 1350, 1800 and 2250 degree days, approximately 20, 40, 60, 80 and 100 days). As the forage deferment increased, the nutritional value decreased more in C4 than in C3 grasses. Short rest periods (approximately 40 days) are recommended for PASDIL and MNESEL, and medium rest periods (approximately 80 days) for ANDLAT and PASNOT. However, the C3 grasses BROAU and NASNEE maintained high P and CP concentrations and may be the most appropriate option for long rest periods (≥100 days). This information is important to manage different Campos grassland communities for the optimal rest period according to the dominant species. Full article

In a continued search for legumes to grow with forage sorghum (FS) [Sorghum bicolor (L.) Moench] in semi-arid environments, studies in 2019 and 2022 at New Mexico State University Rex E. Kirksey Agricultural Science Center at Tucumcari, NM USA, evaluated FS mixed with cowpea [Vigna unguiculata (L.) Walp.], lablab (Lablab purpureus L.), both previously evaluated, and a native legume, big-pod sesbania (Sesbania macrocarpa), for yield and nutritive value in four randomized complete blocks each year. At harvest legume proportions of approximately 15% of the dry matter yield, there were no differences in the yield (mean = 15.97 Mg ha⁻¹, p > 0.40) or land equivalency ratio between sole FS and any mixture or among mixtures; however, the crude protein of FS+Lablab was greater than sole FS, with FS+Cowpea and FS+Sesbania intermediate (67, 77, 87, and 79 g kg⁻¹ for sole FS, FS+Cowpea, FS+Lablab, and FS+Sesbania, respectively; 5% LSD = 14). The neutral detergent fiber digestibility of FS+Sesbania was less than all other treatments (p = 0.0266). Although the sesbania did not improve forage yield or nutritive value when grown with FS and harvested near sesbania maturity, perhaps growing with a shorter season grass and harvesting earlier may show benefit, as sesbania’s nutritive value is known to be greater at earlier stages. Full article

This study aimed to determine the optimal growth stage and additives for producing high quality millet silage through two experiments. Experiment 1: Whole-plant millet from the same field and under uniform management was harvested at the heading, sizing, milking, dough, and full-maturity stages. Then, it was chopped into 2–3 cm segments, vacuum-sealed in plastic bags without any further treatment, stored at 20 °C, and opened after 60 days. The results indicated that the dough stage had the highest water-soluble carbohydrate (WSC) and crude protein (CP) contents. The lactic acid (LA) and acetic acid (AA) contents during the dough and maturity stages were significantly higher than other stages, with the lowest pH observed during the dough stage. Experiment 2: The whole-plant millet was harvested at the dough stage. It was then chopped into 2–3 cm segments using a forage chopper, mixed thoroughly, and subjected to different treatments—inoculation with 10⁶ CFU/g FM of Lactiplantibacillus plantarum (LP), adding of 1% FM sucrose (S), and a combination of Lactiplantibacillus plantarum and sucrose (MIX)—with a control group (CK) receiving an equivalent amount of water. The MIX treatment significantly enhanced the WSC content compared to other treatments (p < 0.05), and both the LP and MIX treatments showed superior LA and AA contents and lactic acid bacteria counts. These additives significantly altered the bacterial community, shifting dominance from Proteobacteria in the CK and raw materials to Firmicutes. Klebsiella dominated the CK group but was significantly reduced in the additive treatments, where Lentilactobacillus became the dominant genus. Therefore, we recommend harvesting millet at the dough stage and adding a mixture of Lactiplantibacillus plantarum and sugar to improve fermentation quality and aerobic stability. Full article

Alfalfa (Medicago L.) is a high-quality perennial leguminous forage with the advantages of salt tolerance, mowing tolerance, high protein content, and other economically valuable characteristics. As the sixth class of plant hormones, brassinosteroids (BRs) play indispensable roles in modulating a variety of plant growth, maturation, and environmental adaptation processes, thereby influencing vegetal expansion and development. Brassinosteroid signal kinases (BSKs) are key cytoplasmic receptor kinases downstream of the BR signaling transduction pathway, participating in plant growth, development, and stress regulation. However, the phylogenetic and expression pattern analyses of the BSK gene family among the five alfalfa species have rarely been reported; in this study, 52 BSK family members were found in the genomes of the five subspecies, and phylogenetic trees were constructed according to protein sequences, allowing us to categorize all BSKs into seven distinct groups. Domain, conserved motif, and exon–intron structural analyses showed that most BSK members were relatively conserved, except for MtBSK3-2, MtBSK7-1, and MtBSK7-2, which may be truncated members. Intra-species collinearity and Ka/Ks analyses showed that purifying selection influenced BSK genes during evolution; most of the cis-acting elements in the promoter region were associated with responses, such as light, defense, and stress, anaerobic induction, MeJA, and abscisic acid. Expression pattern analysis indicated that the majority of alfalfa genes exhibited downregulation after reaching a peak at 0.5 h after treatment with 250 mM NaCl, especially for MsBSK14, MsBSK15, MsBSK17, MsBSK19, and MsBSK21; meanwhile, MsBSK4, MsBSK7, and MsBSK9 increased and were highly expressed at 12 h, demonstrating significantly altered expression patterns under salt stress; furthermore, MsBSK4, MsBSK7, and MsBSK9 exhibited expression specifically in the leaves. qRT-PCR analysis confirmed the expression trends for MsBSK4, MsBSK7, MsBSK9, MsBSK14, MsBSK15, and MsBSK16 matched the transcriptome data. However, the trends for MsBSK17, MsBSK19, and MsBSK21 diverged from the transcriptome data. Our study may provide a foundation for further functional analyses of BSK genes in growth, development, and salt stress tolerance in alfalfa. Full article