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14 pages, 646 KiB  
Review
The Role of Sensor Technologies in Estrus Detection in Beef Cattle: A Review of Current Applications
by Inga Merkelytė, Artūras Šiukščius and Rasa Nainienė
Animals 2025, 15(15), 2313; https://doi.org/10.3390/ani15152313 (registering DOI) - 7 Aug 2025
Abstract
Modern beef cattle reproductive management faces increasing challenges due to the growing global demand for beef. Reproductive efficiency is a critical factor determining the productivity and profitability of beef cattle operations. Optimal reproductive performance in a beef cattle herd is achieved when each [...] Read more.
Modern beef cattle reproductive management faces increasing challenges due to the growing global demand for beef. Reproductive efficiency is a critical factor determining the productivity and profitability of beef cattle operations. Optimal reproductive performance in a beef cattle herd is achieved when each cow produces one calf per year, maintaining a calving interval of 365 days. However, this goal is difficult to achieve, as the gestation period in beef cows lasts approximately 280 days, leaving only 80–85 days for successful conception. Traditional methods, such as visual estrus detection, are becoming increasingly unreliable due to expanding herd sizes and the subjectivity of visual observation. Additionally, silent estrus—where ovulation occurs without noticeable behavioral changes—further complicates the accurate estrous-based identification of the optimal insemination period. To enhance reproductive efficiency, advanced technologies are increasingly being integrated into cattle management. Sensor-based monitoring systems, including accelerometers, pedometers, and ruminoreticular boluses, enable the precise tracking of activity changes associated with the estrous cycle. Furthermore, infrared thermography offers a non-invasive method for detecting body temperature fluctuations, allowing for more accurate estrus identification and optimized timing of insemination. The use of these innovative technologies has the potential to significantly improve reproductive efficiency in beef cattle herds and contribute to overall farm productivity and sustainability. The objective of this review is to examine advancements in smart technologies applied to beef cattle reproductive management, presenting commercially available technologies and recent scientific studies on innovative systems. The focus is on sensor-based monitoring systems and infrared thermography for optimizing reproduction. Additionally, the challenges associated with these technologies and their potential to enhance reproductive efficiency and sustainability in the beef cattle industry are discussed. Despite the benefits of advanced technologies, their implementation in cattle farms is hindered by financial and technical challenges. High initial investment costs and the complexity of data analysis may limit their adoption, particularly in small and medium-sized farms. However, the continuous development of these technologies and their adaptation to farmers’ needs may significantly contribute to more efficient and sustainable reproductive management in beef cattle production. Full article
(This article belongs to the Special Issue Reproductive Management Strategies for Dairy and Beef Cows)
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15 pages, 771 KiB  
Review
Trichoderma: Dual Roles in Biocontrol and Plant Growth Promotion
by Xiaoyan Chen, Yuntong Lu, Xing Liu, Yunying Gu and Fei Li
Microorganisms 2025, 13(8), 1840; https://doi.org/10.3390/microorganisms13081840 (registering DOI) - 7 Aug 2025
Abstract
The genus Trichoderma plays a pivotal role in sustainable agriculture through its multifaceted contributions to plant health and productivity. This review explores Trichoderma’s biological functions, including its roles as a biocontrol agent, plant growth promoter, and stress resilience enhancer. By producing various [...] Read more.
The genus Trichoderma plays a pivotal role in sustainable agriculture through its multifaceted contributions to plant health and productivity. This review explores Trichoderma’s biological functions, including its roles as a biocontrol agent, plant growth promoter, and stress resilience enhancer. By producing various enzymes, secondary metabolites, and volatile organic compounds, Trichoderma effectively suppresses plant pathogens, promotes root development, and primes plant immune responses. This review details the evolutionary adaptations of Trichoderma, which has transitioned from saprotrophism to mycoparasitism and established beneficial symbiotic relationships with plants. It also highlights the ecological versatility of Trichoderma in colonizing plant roots and improving soil health, while emphasizing its role in mitigating both biotic and abiotic stressors. With increasing recognition as a biostimulant and biocontrol agent, Trichoderma has become a key player in reducing chemical inputs and advancing eco-friendly farming practices. This review addresses challenges such as strain selection, formulation stability, and regulatory hurdles and concludes by advocating for continued research to optimize Trichoderma’s applications in addressing climate change, enhancing food security, and promoting a sustainable agricultural future. Full article
(This article belongs to the Special Issue Advances in Plant–Soil–Microbe Interactions)
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21 pages, 1788 KiB  
Article
Investigation, Prospects, and Economic Scenarios for the Use of Biochar in Small-Scale Agriculture in Tropical
by Vinicius John, Ana Rita de Oliveira Braga, Criscian Kellen Amaro de Oliveira Danielli, Heiriane Martins Sousa, Filipe Eduardo Danielli, Newton Paulo de Souza Falcão, João Guerra, Dimas José Lasmar and Cláudia S. C. Marques-dos-Santos
Agriculture 2025, 15(15), 1700; https://doi.org/10.3390/agriculture15151700 - 6 Aug 2025
Abstract
This study investigates the production and economic feasibility of biochar for smallholder and family farms in Central Amazonia, with potential implications for other tropical regions. The costs of construction of a prototype mobile kiln and biochar production were evaluated, using small-sized biomass from [...] Read more.
This study investigates the production and economic feasibility of biochar for smallholder and family farms in Central Amazonia, with potential implications for other tropical regions. The costs of construction of a prototype mobile kiln and biochar production were evaluated, using small-sized biomass from acai (Euterpe oleracea Mart.) agro-industrial residues as feedstock. The biochar produced was characterised in terms of its liming capacity (calcium carbonate equivalence, CaCO3eq), nutrient content via organic fertilisation methods, and ash analysis by ICP-OES. Field trials with cowpea assessed economic outcomes, as well scenarios of fractional biochar application and cost comparison between biochar production in the prototype kiln and a traditional earth-brick kiln. The prototype kiln showed production costs of USD 0.87–2.06 kg−1, whereas traditional kiln significantly reduced costs (USD 0.03–0.08 kg−1). Biochar application alone increased cowpea revenue by 34%, while combining biochar and lime raised cowpea revenues by up to 84.6%. Owing to high input costs and the low value of the crop, the control treatment generated greater net revenue compared to treatments using lime alone. Moreover, biochar produced in traditional kilns provided a 94% increase in net revenue compared to liming. The estimated externalities indicated that carbon credits represented the most significant potential source of income (USD 2217 ha−1). Finally, fractional biochar application in ten years can retain over 97% of soil carbon content, demonstrating potential for sustainable agriculture and carbon sequestration and a potential further motivation for farmers if integrated into carbon markets. Public policies and technological adaptations are essential for facilitating biochar adoption by small-scale tropical farmers. Full article
(This article belongs to the Special Issue Converting and Recycling of Agroforestry Residues)
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23 pages, 2767 KiB  
Article
Sustainable Cotton Production in Sicily: Yield Optimization Through Varietal Selection, Mycorrhizae, and Efficient Water Management
by Giuseppe Salvatore Vitale, Nicolò Iacuzzi, Noemi Tortorici, Giuseppe Indovino, Loris Franco, Carmelo Mosca, Antonio Giovino, Aurelio Scavo, Sara Lombardo, Teresa Tuttolomondo and Paolo Guarnaccia
Agronomy 2025, 15(8), 1892; https://doi.org/10.3390/agronomy15081892 - 6 Aug 2025
Abstract
This study explores the revival of cotton (Gossypium spp. L.) farming in Italy through sustainable practices, addressing economic and water-related challenges by integrating cultivar selection, arbuscular mycorrhizal fungi (AMF) inoculation, and deficit irrigation under organic farming. Field trials evaluated two widely grown [...] Read more.
This study explores the revival of cotton (Gossypium spp. L.) farming in Italy through sustainable practices, addressing economic and water-related challenges by integrating cultivar selection, arbuscular mycorrhizal fungi (AMF) inoculation, and deficit irrigation under organic farming. Field trials evaluated two widely grown Mediterranean cultivars (Armonia and ST-318) under three irrigation levels (I-100: 100% crop water requirement; I-70: 70%; I-30: 30%) across two Sicilian soil types (sandy loam vs. clay-rich). Under I-100, lint yields reached 0.99 t ha−1, while severe deficit (I-30) yielded only 0.40 t ha−1. However, moderate deficit (I-70) maintained 75–79% of full yields, proving a viable strategy. AMF inoculation significantly enhanced plant height (68.52 cm vs. 65.85 cm), boll number (+22.1%), and seed yield (+12.5%) (p < 0.001). Cultivar responses differed: Armonia performed better under water stress, while ST-318 thrived with full irrigation. Site 1, with higher organic matter, required 31–38% less water and achieved superior irrigation water productivity (1.43 kg m−3). Water stress also shortened phenological stages, allowing earlier harvests—important for avoiding autumn rains. These results highlight the potential of combining adaptive irrigation, resilient cultivars, and AMF to restore sustainable cotton production in the Mediterranean, emphasizing the importance of soil-specific management. Full article
(This article belongs to the Section Farming Sustainability)
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23 pages, 1627 KiB  
Article
Sugar Beet Profitability in Lubelskie Province, Poland
by Waldemar Samociuk, Zbigniew Krzysiak, Krzysztof Przystupa and Janusz Zarajczyk
Appl. Sci. 2025, 15(15), 8685; https://doi.org/10.3390/app15158685 (registering DOI) - 6 Aug 2025
Abstract
The work presents a comprehensive analysis and costing of sugar beet cultivation in 2020–2022, for individual farms of the Lublin region. About 120 farms were analyzed. Based on this analysis, the criteria for a model farm were determined and adopted for the calculation [...] Read more.
The work presents a comprehensive analysis and costing of sugar beet cultivation in 2020–2022, for individual farms of the Lublin region. About 120 farms were analyzed. Based on this analysis, the criteria for a model farm were determined and adopted for the calculation of sugar beet production costs. ARIMA process modeling was performed, based on which forecasts were determined for several selected parameters. Customs tariffs introduced by the USA have a drastic impact on the economy. The effects of the COVID19 pandemic may also have a significant impact on the current market situation. Forecasting in the current geopolitical situation is very difficult because of the lack of stationarity of parameters. The financial result obtained by growers is mainly influenced by indirect costs absorbing 61.31% of total costs in 2020. In 2021 and 2022, indirect costs were 61.16% and 59.61% of production income, respectively. Among this group of costs, the largest share is accounted for by the costs of sowing services, sugar beet harvesting, and soil liming amounting from 14.27% to 15.92%. During the analyzed period, sugar beet cultivation remained profitable, with a production profitability index of 1.31 in 2020 and 2021, and 1.10 in 2022. The unit cost of production increased every year. In 2020, it was 14.27% and in 2021, it increased to 15.19%. The unit cost of production in 2022 was the highest, at 23.41%. Sugar beet cultivation is one of the profitable activities in agricultural production, but it is characterized by high production costs, which increased during the years analyzed (2020 to 2022), topping out at 90.87% of total revenue. The information and data presented in this study will be used in the development of a farmer-oriented application and will support the creation of an expert system for sugar beet growers. Cost forecasting will enable farmers to plan their production more effectively. Full article
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43 pages, 1183 KiB  
Review
Harnessing Legume Productivity in Tropical Farming Systems by Addressing Challenges Posed by Legume Diseases
by Catherine Hazel Aguilar, David Pires, Cris Cortaga, Reynaldo Peja, Maria Angela Cruz, Joanne Langres, Mark Christian Felipe Redillas, Leny Galvez and Mark Angelo Balendres
Nitrogen 2025, 6(3), 65; https://doi.org/10.3390/nitrogen6030065 - 5 Aug 2025
Abstract
Legumes are among the most important crops globally, serving as a major food source for protein and oil. In tropical regions, the cultivation of legumes has expanded significantly due to the increasing demand for food, plant-based products, and sustainable agriculture practices. However, tropical [...] Read more.
Legumes are among the most important crops globally, serving as a major food source for protein and oil. In tropical regions, the cultivation of legumes has expanded significantly due to the increasing demand for food, plant-based products, and sustainable agriculture practices. However, tropical environments pose unique challenges, including high temperatures, erratic rainfall, soil infertility, and a high incidence of pests and diseases. Indeed, legumes are vulnerable to infections caused by bacteria, fungi, oomycetes, viruses, and nematodes. This review highlights the importance of legumes in tropical farming and discusses major diseases affecting productivity and their impact on the economy, environment, and lives of smallholder legume farmers. We emphasize the use of legume genetic resources and breeding, and biotechnology innovations to foster resistance and address the challenges posed by pathogens in legumes. However, an integrated approach that includes other cultivation techniques (e.g., crop rotation, rational fertilization, deep plowing) remains important for the prevention and control of diseases in legume crops. Finally, we highlight the contributions of plant genetic resources to smallholder resilience and food security. Full article
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13 pages, 223 KiB  
Article
Preliminary Research on the Efficacy of Selected Herbicides Approved for Use in Sustainable Agriculture Using Spring Cereals as an Example
by Piotr Szulc, Justyna Bauza-Kaszewska, Marek Selwet and Katarzyna Ambroży-Deręgowska
Sustainability 2025, 17(15), 7090; https://doi.org/10.3390/su17157090 - 5 Aug 2025
Abstract
The objective of this study was to evaluate the efficacy of selected herbicides permitted for use in sustainable agriculture, specifically targeting spring rye and spring barley in a no-till farming system. The application of chemical herbicide protection in the cultivation of spring rye [...] Read more.
The objective of this study was to evaluate the efficacy of selected herbicides permitted for use in sustainable agriculture, specifically targeting spring rye and spring barley in a no-till farming system. The application of chemical herbicide protection in the cultivation of spring rye and barley significantly increased the yield and improved the quality parameters of the harvested grain, with the most pronounced effect observed in spring barley. The effectiveness of the herbicide treatment in reducing the number of weeds was 99.4% for spring rye and 82.39% for spring barley. The study demonstrated that the application of chemical herbicide protection had a positive impact on the quality parameters of spring barley grain. Both the thousand-grain weight and protein content were significantly higher in the grain collected from protected plots compared to the control plots. By utilizing herbicides permitted for use in integrated production (IP) in a sustainable manner, we protect the environment while minimizing the impact on crop yields and maintaining the quality of the harvested produce. Full article
17 pages, 7024 KiB  
Article
Proteomic Analysis of Differentially Expressed Plasma Exosome Proteins in Heat-Stressed Holstein cows
by Shuwen Xia, Yingying Jiang, Wenjie Li, Zhenjiang An, Yangyang Shen, Qiang Ding and Kunlin Chen
Animals 2025, 15(15), 2286; https://doi.org/10.3390/ani15152286 - 5 Aug 2025
Viewed by 31
Abstract
Heat stress in dairy cows, caused by high temperature and humidity during summer, has led to significant declines in milk production and severe economic losses for farms. Exosomes—extracellular vesicles carrying bioactive molecules—are critical for intercellular communication and immunity but remain understudied in heat-stressed [...] Read more.
Heat stress in dairy cows, caused by high temperature and humidity during summer, has led to significant declines in milk production and severe economic losses for farms. Exosomes—extracellular vesicles carrying bioactive molecules—are critical for intercellular communication and immunity but remain understudied in heat-stressed Holstein cows. In this study, we extracted exosomes from three heat-stressed (HS) cows and three non-heat-stressed (Ctr) cows and employed proteomics to analyze plasma exosomes. We identified a total of 28 upregulated and 18 downregulated proteins in the HS group compared to the control group. Notably, we observed a significant upregulation of key protein groups, including cytoskeletal regulators, signaling mediators, and coagulation factors, alongside the downregulation of HP-25_1. These differentially expressed proteins demonstrate strong potential as heat stress biomarkers. GO and KEGG analyses linked the differentially expressed proteins to actin cytoskeleton regulation and endoplasmic reticulum pathways. Additionally, protein–protein interaction (PPI) analysis revealed the PI3K-Akt signaling pathway as a central node in the cellular response to heat stress. These findings establish plasma exosomes as valuable biospecimens, provide valuable insights into the molecular mechanisms of heat stress response, and may contribute to the development of precision breeding strategies for enhanced thermal resilience in dairy herds. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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10 pages, 386 KiB  
Article
Certified Seed Use Enhances Yield Stability in Cereal Production Under Temperate Climate Conditions
by Patrycja Ojdowska, Tadeusz Oleksiak, Marcin Studnicki and Marzena Iwańska
Agronomy 2025, 15(8), 1886; https://doi.org/10.3390/agronomy15081886 - 5 Aug 2025
Viewed by 94
Abstract
In the face of growing demand for food and climate change, ensuring the stability and height of crop yields is becoming a key challenge for modern agriculture. One of the solutions supporting the sustainable development of crop production is the use of certified [...] Read more.
In the face of growing demand for food and climate change, ensuring the stability and height of crop yields is becoming a key challenge for modern agriculture. One of the solutions supporting the sustainable development of crop production is the use of certified seed. The aim of this study was to assess the impact of using certified seed on the level and stability of yields of three cereal species: winter wheat, winter triticale and spring barley, in temperate climate conditions. Data came from surveys conducted on over 8000 farms in six agroecoregions of Poland in 2021–2023. The analysis showed significantly higher yields on farms using certified seed for all species studied. Additionally, greater yield stability (lower values of Shukla variance and Wricke ecovalence) was noted in the case of using certified seeds, especially in region IV. This indicates the positive impact of certified seeds (e.g., genetic purity, health, and vigor) on the efficiency and resilience of agricultural systems. This phenomenon is of particular importance in the context of climate change and may be an important element of risk management strategies in agriculture. Full article
(This article belongs to the Special Issue Genotype × Environment Interactions in Crop Production—2nd Edition)
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17 pages, 1459 KiB  
Article
Assessing Controlled Traffic Farming as a Precision Agriculture Strategy for Minimising N2O Losses
by Bawatharani Raveendrakumaran, Miles Grafton, Paramsothy Jeyakumar, Peter Bishop and Clive Davies
Nitrogen 2025, 6(3), 63; https://doi.org/10.3390/nitrogen6030063 - 4 Aug 2025
Viewed by 158
Abstract
Intensive vegetable farming emits high nitrous oxide (N2O) due to traffic-induced compaction, highlighting the need for preventing nitrogen (N) losses through better traffic management. This study examined the effects of Controlled Traffic Farming (CTF) and Random Traffic Farming (RTF) on N [...] Read more.
Intensive vegetable farming emits high nitrous oxide (N2O) due to traffic-induced compaction, highlighting the need for preventing nitrogen (N) losses through better traffic management. This study examined the effects of Controlled Traffic Farming (CTF) and Random Traffic Farming (RTF) on N2O emissions using intact soil cores (diameter: 18.7 cm; depth: 25 cm) collected from a vegetable production system in Pukekohe, New Zealand. Soil cores from CTF beds, CTF tramlines, and RTF plots were analysed under fertilised (140 kg N/ha) and unfertilised conditions. N2O fluxes were monitored over 58 days using gas chambers. The fertilised RTF system significantly (p < 0.05) increased N2O emissions (5.4 kg N2O–N/ha) compared to the unfertilised RTF system (1.53 kg N2O–N/ha). The emission from fertilised RTF was 46% higher than the maximum N2O emissions (3.7 kg N2O–N/ha) reported under New Zealand pasture conditions. The fertilised CTF system showed a 31.6% reduction in N2O emissions compared to fertilised RTF and did not differ significantly from unfertilised CTF. In general, CTF has demonstrated some resilience against fertiliser-induced N2O emissions, indicating the need for further investigation into its role as a greenhouse gas mitigation strategy. Full article
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21 pages, 1369 KiB  
Article
Optimizing Cold Food Supply Chains for Enhanced Food Availability Under Climate Variability
by David Hernandez-Cuellar, Krystel K. Castillo-Villar and Fernando Rey Castillo-Villar
Foods 2025, 14(15), 2725; https://doi.org/10.3390/foods14152725 - 4 Aug 2025
Viewed by 217
Abstract
Produce supply chains play a critical role in ensuring fruits and vegetables reach consumers efficiently, affordably, and at optimal freshness. In recent decades, hub-and-spoke network models have emerged as valuable tools for optimizing sustainable cold food supply chains. Traditional optimization efforts typically focus [...] Read more.
Produce supply chains play a critical role in ensuring fruits and vegetables reach consumers efficiently, affordably, and at optimal freshness. In recent decades, hub-and-spoke network models have emerged as valuable tools for optimizing sustainable cold food supply chains. Traditional optimization efforts typically focus on removing inefficiencies, minimizing lead times, refining inventory management, strengthening supplier relationships, and leveraging technological advancements for better visibility and control. However, the majority of models rely on deterministic approaches that overlook the inherent uncertainties of crop yields, which are further intensified by climate variability. Rising atmospheric CO2 concentrations, along with shifting temperature patterns and extreme weather events, have a substantial effect on crop productivity and availability. Such uncertainties can prompt distributors to seek alternative sources, increasing costs due to supply chain reconfiguration. This research introduces a stochastic hub-and-spoke network optimization model specifically designed to minimize transportation expenses by determining optimal distribution routes that explicitly account for climate variability effects on crop yields. A use case involving a cold food supply chain (CFSC) was carried out using several weather scenarios based on climate models and real soil data for California. Strawberries were selected as a representative crop, given California’s leading role in strawberry production. Simulation results show that scenarios characterized by increased rainfall during growing seasons result in increased yields, allowing distributors to reduce transportation costs by sourcing from nearby farms. Conversely, scenarios with reduced rainfall and lower yields require sourcing from more distant locations, thereby increasing transportation costs. Nonetheless, supply chain configurations may vary depending on the choice of climate models or weather prediction sources, highlighting the importance of regularly updating scenario inputs to ensure robust planning. This tool aids decision-making by planning climate-resilient supply chains, enhancing preparedness and responsiveness to future climate-related disruptions. Full article
(This article belongs to the Special Issue Climate Change and Emerging Food Safety Challenges)
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23 pages, 857 KiB  
Article
Study of the Impact of Agricultural Insurance on the Livelihood Resilience of Farmers: A Case Study of Comprehensive Natural Rubber Insurance
by Jialin Wang, Yanglin Wu, Jiyao Liu and Desheng Zhang
Agriculture 2025, 15(15), 1683; https://doi.org/10.3390/agriculture15151683 - 4 Aug 2025
Viewed by 219
Abstract
Against the backdrop of increasingly frequent extreme weather events and heightened market price volatility, investigating the relationship between agricultural insurance and farmers’ livelihood resilience is crucial for ensuring rural socioeconomic stability. This study utilizes field survey data from 1196 households across twelve county-level [...] Read more.
Against the backdrop of increasingly frequent extreme weather events and heightened market price volatility, investigating the relationship between agricultural insurance and farmers’ livelihood resilience is crucial for ensuring rural socioeconomic stability. This study utilizes field survey data from 1196 households across twelve county-level divisions (three cities and nine counties) from China’s Hainan and Yunnan provinces, specifically in natural rubber-producing regions. Using propensity score matching (PSM), we empirically examine agricultural insurance’s impact on household livelihood resilience. The results demonstrate that agricultural insurance increased the effect on farmers’ livelihood resilience by 1%. This effect is particularly pronounced among recently poverty-alleviated households and large-scale farming operations. Furthermore, the analysis highlights the mediating roles of credit availability, adoption of agricultural production technologies, and production initiative in strengthening insurance’s positive impact. Therefore, policies should be refined and expanded, combining agricultural insurance with credit support and agricultural technology extension to leverage their value and ensure the sustainable development of farm households. Full article
(This article belongs to the Section Agricultural Economics, Policies and Rural Management)
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13 pages, 2281 KiB  
Article
Amphipathic Alpha-Helical Peptides AH1 and AH3 Facilitate Immunogenicity of Enhanced Green Fluorescence Protein in Rainbow Trout (Oncorhynchus mykiss)
by Kuan Chieh Peng and Ten-Tsao Wong
J. Mar. Sci. Eng. 2025, 13(8), 1497; https://doi.org/10.3390/jmse13081497 - 4 Aug 2025
Viewed by 143
Abstract
Vaccination is the most effective method to counteract infectious diseases in farmed fish. It secures aquaculture production and safeguards the wild stock and aquatic ecosystem from catastrophic contagious diseases. In vaccine development, recombinant subunit vaccines are favorable candidates since they can be economically [...] Read more.
Vaccination is the most effective method to counteract infectious diseases in farmed fish. It secures aquaculture production and safeguards the wild stock and aquatic ecosystem from catastrophic contagious diseases. In vaccine development, recombinant subunit vaccines are favorable candidates since they can be economically produced in large quantities without growing many pathogens, as in inactivated or attenuated vaccine production. However, recombinant subunit vaccines are often weak or deficient in immunogenicity, resulting in inadequate defenses against infections. Technologies that can increase the immunogenicity of recombinant subunit vaccines are in desperate need. Enhanced green fluorescence protein (EGFP) has a low antigenicity and is susceptible to folding changes and losing fluorescence after fusing with other proteins. Using these valuable features of EGFP, we comprehend two amphipathic alpha-helical peptides, AH1 and AH3, derived from Hepatitis C virus and Influenza A virus, respectively, that can induce high immune responses of their fused EGFP in fish without affecting their folding. AH3-EGFP has the most elevated cell binding, significantly 62% and 36% higher than EGFP and AH1-EGFP, respectively. Immunizations with AH1-EGFP or AH3-EGFP significantly induced higher anti-EGFP antibody levels 300–500-fold higher than EGFP immunization after the boost injection in rainbow trout. Our results suggest that AH1 and AH3 effectively increase the immunogenicity of EGFP without influencing its structure. Further validation of their value in other recombinant proteins is necessary to demonstrate their broader utility in enhancing the immunogenicity of subunit vaccines. We also suggest that EGFP and its variants are promising candidates for initially screening proper immunogenicity-enhancing peptides or proteins to advance recombinant subunit vaccine development. Full article
(This article belongs to the Section Marine Aquaculture)
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19 pages, 5733 KiB  
Article
The Production Optimization of a Thermostable Phytase from Bacillus subtilis SP11 Utilizing Mustard Meal as a Substrate
by Md. Al Muid Khan, Sabina Akhter, Tanjil Arif, Md. Mahmuduzzaman Mian, Md. Arafat Al Mamun, Muhammad Manjurul Karim and Shakila Nargis Khan
Fermentation 2025, 11(8), 452; https://doi.org/10.3390/fermentation11080452 - 3 Aug 2025
Viewed by 228
Abstract
Phytate, an antinutritional molecule in poultry feed, can be degraded by applying phytase, but its use in low- and middle-income countries is often limited due to importation instead of local production. Here, inexpensive raw materials were used to optimize the production of a [...] Read more.
Phytate, an antinutritional molecule in poultry feed, can be degraded by applying phytase, but its use in low- and middle-income countries is often limited due to importation instead of local production. Here, inexpensive raw materials were used to optimize the production of a thermostable phytase from an indigenous strain of Bacillus subtilis SP11 that was isolated from a broiler farm in Dhaka. SP11 was identified using 16s rDNA and the fermentation of phytase was optimized using a Plackett–Burman design and response surface methodology, revealing that three substrates, including the raw material mustard meal (2.21% w/v), caused a maximum phytase production of 436 U/L at 37 °C and 120 rpm for 72 h, resulting in a 3.7-fold increase compared to unoptimized media. The crude enzyme showed thermostability up to 80 °C (may withstand the feed pelleting process) with an optimum pH of 6 (near pH of poultry small-intestine), while retaining 96% activity at 41 °C (the body temperature of the chicken). In vitro dephytinization demonstrated its applicability, releasing 978 µg of inorganic phosphate per g of wheat bran per hour. This phytase has the potential to reduce the burden of phytase importation in Bangladesh by making local production and application possible, contributing to sustainable poultry nutrition. Full article
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15 pages, 3854 KiB  
Article
PVC Inhibits Radish (Raphanus sativus L.) Seedling Growth by Interfering with Plant Hormone Signal Transduction and Phenylpropanoid Biosynthesis
by Lisi Jiang, Zirui Liu, Wenyuan Li, Yangwendi Yang, Zirui Yu, Jiajun Fan, Lixin Guo, Chang Guo and Wei Fu
Horticulturae 2025, 11(8), 896; https://doi.org/10.3390/horticulturae11080896 (registering DOI) - 3 Aug 2025
Viewed by 233
Abstract
Polyvinyl chloride (PVC) is commonly employed as mulch in agriculture to boost crop yields. However, its toxicity is often overlooked. Due to its chemical stability, resistance to degradation, and the inadequacy of the recycling system, PVC tends to persist in farm environments, where [...] Read more.
Polyvinyl chloride (PVC) is commonly employed as mulch in agriculture to boost crop yields. However, its toxicity is often overlooked. Due to its chemical stability, resistance to degradation, and the inadequacy of the recycling system, PVC tends to persist in farm environments, where it can decompose into microplastics (MPs) or nanoplastics (NPs). The radish (Raphanus sativus L.) was chosen as the model plant for this study to evaluate the underlying toxic mechanisms of PVC NPs on seedling growth through the integration of multi-omics approaches with oxidative stress evaluations. The results indicated that, compared with the control group, the shoot lengths in the 5 mg/L and 150 mg/L treatment groups decreased by 33.7% and 18.0%, respectively, and the root lengths decreased by 28.3% and 11.3%, respectively. However, there was no observable effect on seed germination rates. Except for the peroxidase (POD) activity in the 150 mg/L group, all antioxidant enzyme activities and malondialdehyde (MDA) levels were higher in the treated root tips than in the control group. Both transcriptome and metabolomic analysis profiles showed 2075 and 4635 differentially expressed genes (DEGs) in the high- and low-concentration groups, respectively, and 1961 metabolites under each treatment. PVC NPs predominantly influenced seedling growth by interfering with plant hormone signaling pathways and phenylpropanoid production. Notably, the reported toxicity was more evident at lower concentrations. This can be accounted for by the plant’s “growth-defense trade-off” strategy and the manner in which nanoparticles aggregate. By clarifying how PVC NPs coordinately regulate plant stress responses via hormone signaling and phenylpropanoid biosynthesis pathways, this research offers a scientific basis for assessing environmental concerns related to nanoplastics in agricultural systems. Full article
(This article belongs to the Special Issue Stress Physiology and Molecular Biology of Vegetable Crops)
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