Search Results (87)

Soil quality assessment plays a critical role in promoting sustainable land management, particularly in fragile steppe ecosystems. This study provides a comprehensive geoecological evaluation of heavy metal contamination (Pb, Cd, Zn, Cu, Co, Ni, Fe, and Mn) in soils across five districts of the Akmola region, Kazakhstan. The assessment incorporates multiple integrated pollution indices, including the geochemical pollution index (Igeo), pollution coefficient (CF), ecological risk index (Er), pollution load index (PLI), and integrated pollution index (Zc). Spatial analysis combined with multivariate statistical techniques (PCA and clustering analysis) was used to identify pollutant distribution patterns and differentiate areas by risk levels. The findings reveal generally low to moderate contamination, with cadmium (Cd) posing the highest environmental risk due to its elevated toxic response coefficient, despite its low concentration. The study also explores the connection between current soil conditions and historical land-use changes, particularly those associated with the Virgin Lands Campaign of the mid-20th century. The highest PLI values were recorded in the Yesil and Atbasar districts (7.88 and 7.54, respectively), likely driven by intensive agricultural activity and lithological factors. PCA and cluster analysis revealed distinct spatial groupings, reflecting heterogeneity in both the sources and distribution of soil pollutants. Full article

China’s rural rejuvenation rationale reveals that domestic waste management (DWM), as a gateway to habitat enhancement, is a critical policy pathway for achieving sustainable rural development. This paper analyzes the influence of capital endowment (CE) and environmental literacy (EL) on farmers’ willingness to pay (WTP) for DWM through a binary logistic model, focusing on the Yangtze River Delta region, with 571 farmers contributing validated responses. It also conducts a more in-depth exploration of the regulatory role of EL and the influence of CE on WTP for DWM. The findings are as follows: (1) CE and sub-dimensions of economic capital and psychological capital yield a substantial positive effect on WTP for DWM. (2) CE and sub-dimensions of economic capital, human capital, and psychological capital yield a substantial positive effect on LOP for DWM. (3) EL and sub-dimensions of ER, EP, and EKS exert a notably positive influence on WTP and LOP for DWM. (4) EL functions as a moderator in the effect of CE on WTP for DWM. Based on this, this paper puts forward some policy suggestions to improve farmers’ WTP for DWM from two aspects: optimize the structure of farmers’ capital endowment and foster a positive climate for the entire society to safeguard the environment and strengthen the environmental literacy education system. Full article

Volatile organic compounds (VOCs) in urine headspace are potential biomarkers for different medical conditions, as canines can detect human diseases simply by smelling VOCs. Because dogs can detect disease-specific VOCs, gas chromatography–mass spectrometry (GC–MS) systems may be able to differentiate medical conditions with enhanced accuracy and precision, given they have unprecedented efficiency in separating, quantifying, and identifying VOCs in urine. Advancements in instrumentation have permitted the development of portable GC–MS systems that analyze VOCs at the point of care, but these are designed for environmental monitoring, emergency response, and manufacturing/processing. The purpose of this study is to repurpose the HAPSITE^® ER portable GC–MS for identifying urinary VOC biomarkers. Method development focused on optimizing sample preparation, off-column conditions, and instrumental parameters that may affect performance. Once standardized, the method was used to analyze a urine standard (n = 10) to characterize intra-day reproducibility. To characterize inter-day performance, n = 3 samples each from three volunteers (and the standard) were analyzed each day for a total of four days (n = 48 samples). Results showed the method could detect VOC signals with adequate reproducibility and distinguish VOC profiles from different volunteers with 100% accuracy. Full article

Low back pain is a widespread condition that significantly impacts quality of life, with intervertebral disc degeneration (IDD) being a major contributing factor. However, the underlying mechanisms of IDD remain poorly understood, necessitating further investigation. Environmental risk factors, such as mechanical stress and cigarette smoke, elevate reactive oxygen species levels from both endogenous and exogenous sources, leading to redox imbalance and oxidative stress. The endoplasmic reticulum (ER) and mitochondria, two key organelles responsible for protein folding and energy production, respectively, are particularly vulnerable to oxidative stress. Under oxidative stress conditions, ER stress and mitochondrial dysfunction occur, resulting in unfolded protein response activation, impaired biosynthetic processes, and disruptions in the tricarboxylic acid cycle and electron transport chain, ultimately compromising energy metabolism. Prolonged and excessive ER stress can further trigger apoptosis through ER–mitochondrial crosstalk. Given the unique microenvironment of the intervertebral disc (IVD)—characterized by hypoxia, glucose starvation, and region-specific cellular heterogeneity—the differential effects of environmental stressors on distinct IVD cell populations require further investigation. This review explores the potential mechanisms through which environmental risk factors alter IVD cell activities, contributing to IDD progression, and discusses future therapeutic strategies aimed at mitigating disc degeneration. Full article

Rice brown spot disease, caused by Bipolaris oryzae, is a significant fungal disease that poses a major threat to global rice production. Despite its widespread impact, genomic studies of B. oryzae remain limited, particularly those involving high-quality genomic data. In this study, we performed whole-genome sequencing of the B. oryzae strain RBD1, which was isolated from the demonstration field for upland rice cultivation in Haozhiba Village, Lancang County, Pu’er City, Yunnan Province, China, using a combination of second-generation Illumina sequencing and third-generation Single-Molecule Real-Time (SMRT) sequencing. The assembled genome was 37.5 Mb in size with a G + C content of 49.39%, containing 42 contigs with a contig N50 of 2.0 Mb. Genomic analysis identified genes related to carbon, nitrogen, and lipid metabolism, highlighting the strain’s metabolic flexibility under diverse environmental conditions and host interactions. Additionally, we identified pathogenicity-related genes involved in MAPK signaling, G protein signaling, and oxidative stress responses. Under 1.2 M sorbitol-induced osmotic stress, we observed significant differences in growth responses between RBD1 and the rice blast fungus Magnaporthe oryzae H7. Transcriptomic analysis using Illumina sequencing revealed that RBD1 responds to osmotic stress by enhancing carbohydrate metabolism, fatty acid degradation, and amino acid synthesis, while H7 primarily relies on protein synthesis to enhance growth tolerance. This study provides a valuable foundation for understanding the pathogenic mechanisms of rice brown spot and future disease control strategies. Full article

Excessive alcohol consumption significantly impacts human health, particularly the brain, due to its susceptibility to oxidative stress, which contributes to neurodegenerative conditions. Alcohol metabolism in the brain occurs primarily via catalase, followed by CYP2E1 pathways. Excess alcohol metabolized by CYP2E1 generates reactive oxygen/nitrogen species (ROS/RNS), leading to cell injury via altering many different pathways. Elevated oxidative stress impairs autophagic processes, increasing post-translational modifications and further exacerbating mitochondrial dysfunction and ER stress, leading to cell death. The literature highlights that alcohol-induced oxidative stress disrupts autophagy and mitophagy, contributing to neuronal damage. Key mechanisms include mitochondrial dysfunction, ER stress, epigenetics, and the accumulation of oxidatively modified proteins, which lead to neuroinflammation and impaired cellular quality control. These processes are exacerbated by chronic alcohol exposure, resulting in the suppression of protective pathways like NRF2-mediated antioxidant responses and increased susceptibility to neurodegenerative changes in the brain. Alcohol-mediated neurotoxicity involves complex interactions between alcohol metabolism, oxidative stress, and autophagy regulation, which are influenced by various factors such as drinking patterns, nutritional status, and genetic/environmental factors, highlighting the need for further molecular studies to unravel these mechanisms and develop targeted interventions. Full article

Agricultural systems are particularly impacted by global climate change (CC), responsible for the introduction of multiple environmental stressors negatively affecting plant growth. Soil microbial communities are crucial in agricultural practices, influencing crop performance and soil health. Human activities and CC threaten soil microbial biodiversity, leading to soil quality degradation and decreasing plant health and productivity. Among plant-beneficial microorganisms, mycorrhizal fungi are widespread in terrestrial ecosystems, including agroecosystems, and they play a key role by enhancing plants’ fitness and resilience to both abiotic and biotic stresses. Therefore, exploring the role of mycorrhizal symbiosis in sustainable agriculture has become increasingly critical. Moreover, the application of mycorrhizal bioinoculants could reduce dependence on inorganic fertilizers, enhance crop yield, and support plants in overcoming environmental stresses. This review, after briefly introducing taxonomy, morphology and mechanisms supporting the symbiosis establishment, reports the roles of mycorrhizal fungi and their associated bacteria in improving plant nutrition and mitigating CC-induced abiotic stresses such as drought and salinity, also giving specific examples. The focus is on arbuscular mycorrhizal fungi (AMF), but ericoid mycorrhizal (ErM) fungi are also considered as promising microorganisms for a sustainable agricultural model. New emerging concepts are illustrated, such as the role of AMF hyphosphere in acting as a preferential niche to host plant growth-promoting bacteria and the potential of ErM fungi to improve plant performance on Ericaceae plants but also on non-host plants, behaving as endophytes. Finally, the potential and limitations of mycorrhizal-based bioinoculants are discussed as possible alternatives to chemical-based products. To this aim, possible ways to overcome problems and limitations to their use are discussed such as proper formulations, the systematic check of AMF propagule viability and the application of suitable agronomical practices in the field. Full article

Water scarcity is a growing global challenge, requiring effective management strategies to ensure sustainable water use, particularly in regions like West Dorset, U.K., where rising prices and environmental pressures make sustainable consumption practices essential. This study develops and empirically tests an integrated theoretical framework combining Prospect Theory (PT) and Social Cognitive Theory (SCT) to examine the mechanisms driving household water conservation. Using structural equation modelling on data from 429 residents in West Dorset (response rate 78.3%), we investigated how water pricing (WP) and perceived water risk (PWR) influence sustainable water consumption through individual innovativeness (II) and community engagement (CE), with environmental regulations (ERs) as a moderator. The results revealed significant positive effects of WP and PWR on individual innovativeness, which, in turn, strongly predicted community engagement. The relationship between these factors and sustainable water consumption was significantly moderated by ERs. This study makes three key contributions: Firstly, it empirically validates an integrated PT-SCT framework in water conservation behavior. Secondly, it identifies the crucial mediating role of II in translating price and risk perceptions into community action; and lastly, it demonstrates how regulatory frameworks can enhance or inhibit conservation outcomes. These findings inform policy recommendations for tiered pricing structures, community-based conservation initiatives, and adaptive regulatory frameworks. This study provides a comprehensive model for understanding and promoting sustainable water consumption in water-stressed regions worldwide. Full article

Flexible pulse-by-pulse regulation of sensorimotor synchronization is crucial for voluntarily showing rhythmic behaviors synchronously with external cueing; however, the underpinning neurophysiological mechanisms remain unclear. We hypothesized that the dorsal anterior cingulate cortex (dACC) plays a key role by coordinating both proactive and reactive motor outcomes based on contextual mental imagery. To test our hypothesis, a missing-oddball task in finger-tapping paradigms was conducted in 33 healthy young volunteers. The dynamic properties of the dACC were evaluated by event-related deep-brain activity (ER-DBA), supported by event-related potential (ERP) analysis and behavioral evaluation based on signal detection theory. We found that ER-DBA activation/deactivation reflected a strategic choice of motor control modality in accordance with mental imagery. Reverse ERP traces, as omission responses, confirmed that the imagery was contextual. We found that mental imagery was updated only by environmental changes via perceptual evidence and response-based abductive reasoning. Moreover, stable on-pulse tapping was achievable by maintaining proactive control while creating an imagery of syncopated rhythms from simple beat trains, whereas accuracy was degraded with frequent erroneous tapping for missing pulses. We conclude that the dACC voluntarily regulates rhythmic sensorimotor synchronization by utilizing contextual mental imagery based on experience and by creating novel rhythms. Full article

Over the past three decades, significant efforts have been focused on unraveling congenital intestinal disorders that disrupt the absorption of dietary lipids and fat-soluble vitamins. The primary goal has been to gain deeper insights into intra-enterocyte sites, molecular steps, and crucial proteins/regulatory pathways involved, while simultaneously identifying novel therapeutic targets and diagnostic tools. This research not only delves into specific and rare malabsorptive conditions, such as chylomicron retention disease (CRD), but also contributes to our understanding of normal physiology through the utilization of cutting-edge cellular and animal models alongside advanced research methodologies. This review elucidates how modern techniques have facilitated the decoding of CRD gene defects, the identification of dysfunctional cellular processes, disease regulatory mechanisms, and the essential role of coat protein complex II-coated vesicles and cargo receptors in chylomicron trafficking and endoplasmic reticulum (ER) exit sites. Moreover, experimental approaches have shed light on the multifaceted functions of SAR1B GTPase, wherein loss-of-function mutations not only predispose individuals to CRD but also exacerbate oxidative stress, inflammation, and ER stress, potentially contributing to clinical complications associated with CRD. In addition to dissecting the primary disease pathology, genetically modified animal models have emerged as invaluable assets in exploring various ancillary aspects, including responses to environmental challenges such as dietary alterations, gender-specific disparities in disease onset and progression, and embryonic lethality or developmental abnormalities. In summary, this comprehensive review provides an in-depth and contemporary analysis of CRD, offering a meticulous examination of the CRD current landscape by synthesizing the latest research findings and advancements in the field. Full article

Faced with the dual challenges of environmental pollution and climate change, it is of great significance to study the impact of relevant environmental regulations on the synergistic effect of pollution reduction and carbon emission reduction and their influence mechanisms. Based on a theoretical analysis using the panel data of 30 provinces in China, a spatial econometric model and an intermediary effect model are used to investigate the impact of environmental regulations on the synergistic effect of pollution reduction and carbon reduction and the transmission mechanisms potentially responsible for these effects. The empirical results show the following: (1) The three kinds of environmental regulation effectively facilitate the synergistic effect of pollution reduction and carbon reduction, taking the following order when ranked according to the intensity of their effects: command-type environmental regulation (ER1) > market-based environmental regulation (ER2) > voluntary environmental regulation (ER3). (2) Environmental regulation effectively promotes the synergistic effect of pollution and carbon reduction through the three transmission mechanisms of stimulating technological innovation (TI), industrial structure upgrading (ISU), and restricting foreign direct investment (FDI), which take the following effectiveness order: TI > FDI > ISU. Based on the results of the study, policy suggestions to facilitate pollution reduction and carbon synergies are proposed to help China’s green and low-carbon development. Full article

Translation, adaptation, and validation of instruments for cross-cultural investigation requires a rigorous methodological procedure that should be carefully planned to deliver instruments with adequate reliability and validity. This process was applied to a questionnaire measuring Organizational Citizenship Behavior toward the Environment (OCBE), Organizational Identification (OI), Environmental Responsibility (ER), and Green Human Resources Management (GHRM) in Portuguese and Slovak organizations. Several methodological procedures for cross-cultural adaptation and validation of questionnaires were analyzed, most of which used independent translators, experts’ analysis, and backward translation to the original language. In the present study, a procedure adapted from Beaton et al. (2000) was applied successfully, and the blind backward translations; expert committee; and the two pretests to assess content validity, functional equivalence, and clarity proved to be worthy. The psychometric properties were measured using a sample from Portugal (N = 122) and Slovakia (N = 269). Although employees were perceiving neither a strong environmental responsibility of their organizations nor strong green human resources management, they identified themselves with the organizations and engaged in OCBEs. Comparing both countries, the factorial structure was remarkably similar, highlighting the eco-helping actions and eco-civic engagement of OCBEs. Comparing both countries, the factorial structure was remarkably similar, highlighting the eco-helping actions and eco-civic engagement of OCBEs. The results indicated that the translated instrument was functionally equivalent to the original one, valid (scale CVI/Ave > 0.83), and reliable (Scale Alpha > 0.733) for evaluating the effect of employee practices and organization management in promoting and supporting environmental sustainability. Full article

This study focuses on investigating the dimensions of sustainability and their influence on financial-economic sustainability (FES) in traditional agroforestry systems (TAFS) using the case of the Amazonian Chakra. The main objectives were to analyze the dimensions of sustainability and to establish the causal relationships between these dimensions and the FES. To carry out this research, 330 households in Napo Province that use the Amazonian Chakra system to grow cocoa were selected in order to analyze the relationship between the different dimensions of sustainability and FES in this unique context. The results of the study show that practices related to food security (FS) and business factors (BF) have a positive and significant impact on the FES of cocoa-producing households in the Amazonian Chakra system. These findings support the importance of ensuring the availability and quality of food and promoting responsible business practices in these environments. In contrast, the dimensions of environmental resilience (ER) and biodiversity conservation (BC) showed a negative impact on FES, highlighting an economic-financial imbalance in relation to conservation and environmental resilience actions in the Amazonian Chakra. This study contributes to the knowledge needed to promote agricultural practices that include an equal focus on FES, biodiversity conservation, and environmental resilience practices in a globally significant area, providing valuable information for the design of sustainable agricultural policies and practices in the Amazonian Chakra. Full article

We present a study on the green synthesis of undoped and Er-doped ZnO compounds using Mangifera indica gum (MI). A set of tests were conducted to assess the structure of the material. The tests included X-ray diffraction, Raman, and Fourier-transform infrared spectroscopy. Optical properties were studied using diffuse reflectance and photoluminescence. Morphological and textural investigations were done using SEM images and N₂ adsorption/desorption. Furthermore, photocatalytic tests were performed with methylene blue (MB), yellow eosin (EY), and the pharmaceutical drug ibuprofen (IBU) under UV irradiation. The study demonstrated that replacing the stabilizing agent with Mangifera indica gum is an effective method for obtaining ZnO nanoparticles. Additionally, the energy gap of the nanoparticles exhibits a slight reduction in value. Photoluminescence studies showed the presence of zinc vacancies and other defects in both samples. In the photocatalytic test, the sample containing Er³⁺ exhibited a degradation of 99.7% for methylene blue, 81.2% for yellow eosin, and 52.3% for ibuprofen over 120 min. In the presence of methyl alcohol, the degradation of MB and EY dyes is 16.7% and 55.7%, respectively. This suggests that hydroxyl radicals are responsible for the direct degradation of both dyes. In addition, after the second reuse, the degradation rate for MB was 94.08%, and for EY, it was 82.35%. For the third reuse, the degradation rate for MB was 97.15%, and for EY, it was 17%. These results indicate the significant potential of the new semiconductor in environmental remediation applications from an ecological synthesis. Full article

Maintenance of proteome integrity is essential for cell function and survival in changing cellular and environmental conditions. The endoplasmic reticulum (ER) is the major site for the synthesis of secretory and membrane proteins. However, the accumulation of unfolded or misfolded proteins can perturb ER protein homeostasis, leading to ER stress and compromising cellular function. Eukaryotic organisms have evolved sophisticated and conserved protein quality control systems to ensure protein folding fidelity via the unfolded protein response (UPR) and to eliminate potentially harmful proteins via ER-associated degradation (ERAD) and ER-phagy. In this review, we summarize recent advances in our understanding of the mechanisms of ER protein homeostasis in plants and discuss the crosstalk between different quality control systems. Finally, we will address unanswered questions in this field. Full article