Search Results (7,709)

AlCrFeNi-based high-entropy alloys (HEAs) have attracted considerable interest owing to their adjustable phase constitution and attractive mechanical performance. In this study, AlCr_1.6Fe_xNi_(3.2−x)Si_0.2 HEAs (x = 1.0–2.0) were fabricated by vacuum arc melting to systematically evaluate the influence of the Fe/Ni ratio on phase evolution, microstructural characteristics, and mechanical behavior. The results indicate that, with increasing Fe content, the phase constitution gradually changes from BCC+B2+σ to BCC+B2. Correspondingly, the microstructure evolves from floral and cellular eutectic morphologies to branch-like BCC-rich regions with inter-branch/intercellular eutectic constituents. At the same time, the Vickers hardness decreases from 584.1 HV to 365.7 HV as the Fe content increases. Compression results show a gradual reduction in alloy strength, whereas the deformation ability is noticeably improved. Fracture surface analysis further reveals that the alloys with x ≤ 1.4 exhibit typical brittle fracture features, while those with x ≥ 1.6 display incomplete fracture and enhanced plastic deformation. These results clarify the relationship among Fe/Ni ratio, phase constitution, microstructural evolution, and mechanical properties in AlCrFeNiSi-based HEAs. Full article

Reactive astrocytes are a hallmark of several neurological diseases in multiple sclerosis and experimental demyelination models. Their morphological alterations are commonly assessed by qualitative histopathology, yet quantitative tools are required to better capture astrocytic heterogeneity and to allow correlations with imaging-derived biomarkers. Here, we present a workflow for the quantitative analysis of Glial Fibrillary Acidic Protein (GFAP) network remodeling in astrocytes in the cuprizone model of demyelination. C57BL/6 mice were intoxicated with cuprizone for 3 or 5 weeks to induce progressive demyelination, microglial activation, and reactive astrogliosis. Brain sections were processed for anti-GFAP immunohistochemistry, and individual astrocytes from the stratum oriens of the hippocampus were digitally reconstructed. Diverse parameters of GFAP topology, including soma size, process length, branching order, convex hull area, and ramification index, were extracted using either the commercial Neurolucida^® 360 software or the open-source Simple Neurite Tracer (SNT) plugin in ImageJ. Principal component analysis revealed clear differences between control astrocytes and astrocytes in cuprizone-intoxicated animals, with reactive astrocytes displaying increased numbers of primary processes, enhanced bifurcation, and process complexity. Comparative evaluation of Neurolucida^® 360 and SNT demonstrated that both tools are suitable for astrocyte reconstruction, although Neurolucida^® 360 enabled faster and more detailed tracing. This protocol provides a reproducible pipeline for the quantitative assessment of astrocyte morphology under control and pathological conditions, thereby supporting future efforts to link cellular remodeling to functional outcomes in neuroinflammatory disease models. Full article

Ecosystem service assessment provides a critical basis for optimizing regional ecological management and promoting sustainable development. From the water–land–carbon coupling perspective, this study established a technical framework for quantifying individual services, coupling a composite index, and analyzing multidimensional driving mechanisms. The InVEST model was applied to quantify three core ecosystem services: water yield, habitat quality, and carbon storage. A Composite Ecosystem Service Index (CESI) was constructed through normalization and weighted summation. Multidimensional driving factors were identified using the Optimal Parameter-Based Geographical Detector. Taking Ningxia during 2004–2024 as the study area, the results showed that the CESI exhibited a fluctuating upward trend with significant spatial heterogeneity, characterized by a south–high and north–low pattern. Land use transitions were dominated by bidirectional conversions between cropland and grassland, while impervious area expanded rapidly and barren land decreased overall. The spatial differentiation of CESI was jointly controlled by natural and anthropogenic factors, with land use type, precipitation, and digital elevation model showing the strongest explanatory power, and all two-factor interactions displaying pronounced enhancement effects. This study provides a reproducible framework for ecosystem service assessment in arid and semi-arid regions, supporting ecological restoration, land use optimization, and the coordinated development of ecology and economy under water–land–carbon synergy. Full article

Crustal magnetic fields exert a fundamental control on the structure and dynamics of the Martian ionosphere. In this study, we use in situ ion composition measurements from the MAVEN Neutral Gas and Ion Mass Spectrometer (NGIMS) to investigate how crustal magnetic fields modulated the Martian upper atmosphere during the June 2018 global dust storm. By restricting the analysis to a narrow range of solar zenith angles and altitudes, we isolate magnetic effects from variations driven by solar illumination and vertical structure. We find that the densities of O₂⁺, O⁺, and CO₂⁺ differ systematically between regions of strong and weak crustal magnetic fields, with strong-field regions exhibiting reduced variability consistent with magnetic confinement. Importantly, a substantial fraction of observations located outside traditional geographic masks display ion composition signatures that closely resemble those observed in strong-field regions. Spatial analysis shows that these “strong-like” undetermined observations preferentially occur near known crustal magnetic anomalies, indicating that magnetic influence extends beyond fixed geographic boundaries. These results demonstrate that ion composition provides a sensitive diagnostic of magnetic topology at Mars and reveal the importance of magnetic connectivity in regulating ionospheric structure under extreme atmospheric conditions. Our findings suggest that static geographic classifications may underestimate the true spatial reach of crustal magnetic control during periods of enhanced atmospheric disturbance. Full article

Vitexin is a potent C-glycosyl flavone from mung bean coats with significant antioxidant properties, constrained by poor solubility and bioavailability. In this study, Nanovitexin (NV) was encapsulated within a biocompatible Alginate/Carboxymethyl Cellulose (Alg/CMC) matrix via a modified solvent evaporation technique assisted by chemical cross-linking. The optimized NV exhibited a mean dry particle size of 50–70 nm, high concentration (0.05–0.25 mg/mL), and stability (Zeta potential >30 mV). FT-IR analysis confirmed the successful entrapment via intermolecular interactions. Notably, NV exhibited enhanced activities compared to free vitexin (FV), showing superior DPPH scavenging (IC₅₀ of 115.38 μg/mL) versus FV (IC₅₀ of 226.06 μg/mL). Furthermore, NV demonstrated significantly enhanced in vitro antidiabetic potential, displayed no cytotoxicity towards HepG2 cells, and effectively protected against H₂O₂-induced oxidative stress. The Alg/CMC nanomatrix effectively improves vitexin bioactivity, suggesting promising potential for pharmaceutical and nutraceutical applications. Full article

Evapotranspiration (ET) is a fundamental process within the water cycle and the agricultural water balance, optimizing resource allocation, maintaining soil health, and enhancing ecosystem resilience to climate change. Because ET represents a primary consumptive use of irrigation on agricultural lands, enhancing water-use efficiency and sustainable water management requires accurate estimation of evapotranspiration to support long-term sustainability and productivity. This study offers an effective means to visualize spatial and temporal patterns of reference evapotranspiration (ETo) across various vegetation management practices. This study examined the impacts of agroforestry buffers (ABs), grass buffers (GBs), biofuel crops in an agroforestry watershed (BCa), and biofuel crops in a grass buffer watershed (BCg) on ETo, compared to a corn (Zea mays L.)–soybean (Glycine max L.) rotation (RC) for claypan soil in Northern Missouri, USA. The experimental watersheds were located at the Greenley Memorial Research Center, Missouri, USA. Campbell Scientific sensors and Photosynthetically Active Radiation (PAR) smart sensors were installed to measure net radiation, anemometers, humidity, and air temperature. All instruments were mounted on masts at a height of 2 m above ground level in crop, tree, grass, and biofuel areas. Measured meteorological data were recorded hourly from April to October during 2017 and 2018. Daily ETo predictions were calculated using the Penman–Monteith model. These ETo predictions were displayed across the landscape using Python-based GIS for selected dates (each Saturday) for the watersheds. The methodology was implemented using the software programs of Python 2.7.10 and ArcGIS 10.3.1. The results indicated that ETo increased by 11%, 17%, 18%, and 25% in 2017, and by 7%, 9%, 14%, and 20% in 2018 for AB, BCa, BCg, and GB, respectively, compared to RC management. This process may improve soil water recharge in perennial management systems. Accurate estimation of ET in agricultural regions is critical for understanding water balance, hydrological and ecosystem processes, and climate variability. Given that agriculture constitutes the majority of global water consumption, precise ET estimation is particularly significant for sustainable water management, especially in regions experiencing water scarcity. These outcomes may support effective planning and management of agricultural water resources by enabling optimized irrigation and agricultural production. Full article

Vertical electric sources serve as an effective method for identifying deep hydrocarbon reservoirs. This involves the ability to generate transverse magnetic fields, concentrate currents at reservoir interfaces, and effectively emphasize resistivity anomalies in late-time domains. Marine geological conditions are often complex, marked by rugged topography and intricate structures. This complexity results in highly complicated electromagnetic response features, presenting significant challenges for data interpretation. This research employs the Time-Domain Finite Element Method (TDFEM) using unstructured meshes to accurately discretize complex geological models. Through the formulation of TDFEM equations, we successfully performed three-dimensional forward modeling of VED transient electromagnetic (VSTEM) responses in intricate geological environments. An analysis was conducted on the diffusion mechanisms and spatial distribution characteristics of VSTEM fields located beneath the seabed. A comparative analysis was conducted on the resolution capabilities of different fields stimulated by horizontal and VED sources. The findings show that the Ex provides enhanced boundary identification for the lateral extent of targets, whereas the Ez displays the greatest anomaly contrast, highlighting its exceptional results in anomaly detection. We investigated how complex seabed topography and geological structures affect the resolution of hydrocarbon targets. The research indicates that complex topography significantly influences electromagnetic fields; however, the proposed method can still effectively identify resistive hydrocarbon reservoirs, even in intricate model scenarios, thus confirming its reliability in challenging marine environments. Full article

Cyanobacterial species in the Arabian Peninsula region display a diverse range of potential biotechnological application. This review summarizes the cyanobacteria diversity found in the Peninsula region, the bioactive compounds found in these species, and the several health benefits and applications. The Arabian Peninsula region comprises a wide range of cyanobacteria with representatives from the orders Oscillatoriales, Chroococcales, Stigonematales, and Nostocales. These microorganisms produce specialized metabolites such as photosynthetic pigments, pigment–protein complexes, lipopeptides, phenolic compounds, and unique secondary metabolites. Many of the metabolites offer beneficial biological functions including antioxidants, antibacterial, anti-cancer, anti-inflammatory antiviral, and neuroprotective ones. In addition to the medical-related practices, cyanobacteria in the Peninsula region might have several other applications. Other probable uses include their potential bioremediation capability to remove pollutants or heavy metals, as a potential biohydrogen source for renewable energy, and as biofertilizers and soil enhancement to support sustainable agriculture; other useful applications include bioplastics production (polyhydroxyalkanoates), soil microbiota improvement, and methane reduction. The review highlights the potential diverse biotechnological applications of Arabian Peninsula cyanobacteria toward bioremediation, bioplastics, ecosystem regeneration, biofertilizers, bioenergy, and agro-sustainability, as well as human health. This review highlights the importance of the further exploration and exploitation of these resourceful microorganisms for sustainable development in the Arabian Peninsula region. Full article

RNA-binding proteins (RBPs), specifically those containing K Homology (KH) domains, are critical for post-transcriptional regulation and abiotic stress responsiveness in plants. However, systematic characterization of the KHD gene family in potato (Solanum tuberosum L.) remains unreported. Here, we identified 83 StKHD genes unevenly distributed across 12 potato chromosomes, which clustered into five subgroups with conserved gene structures and motif compositions. Most StKHD proteins were predicted to localize to the nucleus, confirmed experimentally for StKHD-41 via transient expression in Nicotiana benthamiana. Collinearity analysis revealed 23, 22, 19, and 4 orthologous pairs with Arabidopsis, tomato, pepper, and tobacco, respectively. Promoter analysis showed distribution of hormone- and stress-responsive cis-elements, while interaction network analysis predicted 39 StKHDs interacting with 137 proteins. Tissue-specific profiling revealed broad expression of several StKHDs, and specific members displayed consistent expression changes under abiotic stresses, correlating with TC-rich repeat enrichment. RT-qPCR validated that StKHD-41 responded rapidly to JA, moderately to SA/GA, and slowly to ABA, with significant upregulation under drought and salt stress by day 2. This study provides a foundation for understanding StKHD functions and identifies targets for enhancing potato stress resistance. Full article

Hyaluronic acid (HA) hydrogels have attracted increasing interest for biomedical applications due to their tunable properties and biocompatibility. Methods: In this study, hyaluronic acid HA-based hydrogels were developed using two distinct crosslinking strategies: physical crosslinking through poly(vinyl alcohol) (PVA) incorporation and covalent crosslinking via DCC/NHS-mediated reactions. Piroxicam (Px) was included as a model drug to evaluate the drug delivery potential of the resulting systems. The hydrogels were characterized in terms of morphology, swelling behaviour, adhesion, enzymatic degradation, drug release, and in vitro cytocompatibility. Results: The results indicate that formulation parameters significantly influence the overall performance of the systems. PVA-containing hydrogels exhibited higher swelling capacity and improved adhesive properties, while covalently crosslinked networks showed reduced swelling and enhanced structural stability and resistance to enzymatic degradation. Drug release profiles were dependent on network structure, with more compact systems displaying slower release behaviour. In vitro assays suggested that the developed hydrogels are cytocompatible and that drug incorporation influences both release kinetics and cellular response. However, it should be noted that the biological evaluation was performed under simplified in vitro conditions, which primarily reflect specific aspects such as cell viability and migration. Conclusions: This study provides a comparative analysis of physical and covalent crosslinking strategies within a HA platform and highlights how formulation variables influence key physicochemical and biological properties. These findings contribute to the rational design of HA-based hydrogels, although further studies are required to establish their performance in more complex biological environments. Full article

Barley hay (BH), characterized by high crude protein, neutral detergent fiber, and various bioactive compounds, is hypothesized to optimize ruminal microbiota and enhance systemic immune responses in sheep. This study assessed how the graded substitution of traditional forage with BH impacts ruminal fermentation, microbiota composition, and blood immune and antioxidant status in sheep. Forty-five male Dorper × Small-tailed Han crossbred lambs (aged 110 ± 10 days, BW 33.93 ± 1.11 kg) were stratified into five cohorts (n = 9 each), including a control (CON) and four experimental groups (BH25, BH50, BH75, and BH100), where BH replaced 25%, 50%, 75%, and 100% of their forage mixture, respectively. Results showed that BH supplementation consistently increased ruminal acetate concentrations. Specifically, BH75 and BH100 significantly enhanced the acetate-to-propionate ratio (p < 0.05) and concurrently reduced NH₃-N concentrations in BH50 and BH100 (p < 0.05). Regarding ruminal microbiota, BH treatment did not alter alpha diversity but significantly enriched fiber-degrading bacteria, including Clostridia, Bacteroidales, and Prevotella, whereas the CON group favored Eubacterium. Blood analysis indicated a time-dependent modulation of immune and antioxidant markers. At day 30, BH treatment resulted in elevated immunoglobulin A (IgA), immunoglobulin G (IgG), interleukin-6 (IL-6), and malondialdehyde (MDA) levels (p < 0.05), whereas catalase (CAT) concentrations were markedly reduced (p < 0.05). By day 60, BH continued to enhance IgA, IgG, and IL-6 levels; meanwhile, tumor necrosis factor α (TNF-α) levels were up-regulated by BH25-BH75 (p < 0.05), while supplementation with BH75 and BH100 led to a decline in interleukin-4 (IL-4), CAT, and interleukin-10 (IL-10) (p < 0.05). Correlation analysis showed that a positive correlation was observed between total volatile fatty acids and Prevotella UCG-003/001, which conversely displayed a negative relationship with IgM. The norank f Eubacterium coprostanoligenes group showed an inverse association with valerate and IgG, but a positive correlation with interleukin-1β (IL-1β). In parallel, the norank f Bacteroidales RF16 group exhibited an inverse association with NH₃-N and CAT, while unclassified c Clostridia was positively correlated with IL-1β. In conclusion, BH substitution modulates ruminal fermentation and immune responses by enriching fiber-degrading bacteria, though it may trigger oxidative stress. Given that BH100 maximized acetate and immunoglobulin production while minimizing MDA levels, complete replacement appears superior to partial substitution. Full article

High lactate concentration is a hallmark of the tumor microenvironment (TME). Regulatory T cells (Tregs) exhibit unique metabolic adaptability to this lactate-rich environment, yet the underlying mechanisms remain incompletely understood. Here, we demonstrate that the monocarboxylate transporter MCT4 is upregulated in tumor-infiltrating Tregs and mediates direct lactate uptake. Using Treg-specific conditional knockout (cKO) mice, we show that MCT4 deficiency does not affect basal Treg development but abrogates lactate-induced Foxp3 stabilization and impairs Treg suppressive function. Mechanistically, MCT4-mediated lactate uptake promotes the lactylation of Foxp3 at lysine 277 (K277), which competitively inhibits its ubiquitination, thereby enhancing Foxp3 protein stability and nuclear localization. Nuclear Foxp3 subsequently interacts with IRF3 to promote IL-10 transcription and secretion. In the B16 melanoma model, MCT4-deficient Tregs display compromised stability and reduced tumor infiltration, leading to enhanced CD8⁺ T cell effector function and attenuated tumor growth. Collectively, our findings reveal that MCT4-mediated lactate uptake sustains Treg stability and function through Foxp3 lactylation, identifying MCT4 as a potential therapeutic target for modulating Treg activity in cancer. Full article

Auranofin (AF), an FDA-approved drug for rheumatoid arthritis, exhibits strong antibacterial activity against Gram-positive bacteria, while Gram-negative species remain largely tolerant. This study assessed the antimicrobial activity of AF and three analogues against clinically relevant Gram-negative pathogens and explored tolerance mechanisms in Pseudomonas aeruginosa. Broth microdilution assays were performed on reference strains and clinical isolates of Escherichia coli, Klebsiella pneumoniae, and P. aeruginosa. Synergy studies with the most active analogue, PEt₃AuCl (AF-Cl), were conducted against P. aeruginosa using polymyxin B (PMB), two efflux-pump inhibitors, and two glutathione (GSH) depletors. Gold compounds showed MICs between 4 and >64 µg/mL, with AF-Cl displaying the highest activity. AF-Cl activity was markedly enhanced by PMB and efflux-pump inhibitors, indicating that outer membrane permeability and efflux contribute to tolerance. Additionally, GSH depletion significantly potentiated AF-Cl, implicating redox homeostasis in resistance. Overall, AF-Cl shows potential against Gram-negative bacteria when combined with agents targeting membrane integrity, efflux systems, or redox balance, supporting combinatorial strategies to overcome resistance in P. aeruginosa and related pathogens. Full article

Verticillium wilt, caused by Verticillium dahliae, is a devastating disease that severely threatens cotton production worldwide. The long-term survival of the pathogen in soil and the limited availability of resistant cultivars make effective control strategies challenging. Although the fungal cross-kingdom RNA VdsR-1 has been reported to delay floral transition and prolong vegetative growth, the underlying plant regulatory mechanisms remain largely unclear. Here, we show that the transcription factor AtTOE1, a target of ath-miR172b-3p, displays altered expression in response to changes in ath-miR172b-3p levels during V. dahliae inoculation, coinciding with coordinated changes in plant immune-related and developmental responses. Increased AtTOE1 expression is correlated with enhanced disease resistance, reduced pathogen colonization, and delayed floral transition. Furthermore, our results indicate that the VdsR-1/AtSPL13A module is associated with modulation of AtTOE1 expression via ath-miR172b-3p, suggesting the involvement of a cross-kingdom RNA-related regulatory framework linking plant immunity and development. Notably, this regulatory relationship is also observed in cotton, indicating evolutionary conservation across plant species. Together, our findings highlight TOE1 as a potential integrator of defense and growth-related processes during pathogen challenge and provide insights that may inform strategies to improve resistance to V. dahliae in cotton and other crops. Full article

A multilayer gradient composite membrane was fabricated on a PES ultrafiltration substrate through layer-by-layer assembly of chitosan (CS) and montmorillonite (MMT), followed by Ca²⁺ crosslinking. The designed architecture forms a multi-layer gradient composite membrane through successive self-assembly, aiming to balance adsorption, interfacial transport and structural stability. SEM observations showed a clear stratified configuration with relatively uniform thickness distribution, including a relatively dense MMT-rich surface layer and a porous PES support that preserved mass-transfer channels. FTIR confirmed the introduction of hydroxyl/amino-containing CS and aluminosilicate-related MMT species onto the membrane surface, indicating successful incorporation of both organic and inorganic components. TG–DTG results further suggested enhanced thermal stability arising from the cooperative effect of the inorganic lamellae and the polymer framework. In dynamic tests, the membrane displayed concentration-responsive adsorption behavior toward gatifloxacin, ciprofloxacin and ofloxacin, and different pollutants reached equilibrium or quasi-steady states at different rates. Comparative kinetic results at the same initial concentration showed that diclofenac, gatifloxacin and ciprofloxacin approached stable plateaus much faster, whereas ofloxacin increased slowly and did not reach an obvious plateau within the tested period. These results indicate that pollutant removal was jointly governed by interfacial interactions, gradient-layer diffusion resistance and overall transport behavior rather than by concentration alone. Overall, the layer-by-layer strategy provided a controllable route for constructing gradient functional layers on PES membranes, demonstrating potential for advanced treatment of antibiotic-containing wastewater and related pharmaceutical effluents. Full article