Search Results (185)

The circadian cycle regulates metabolism in response to external stimuli, such as light exposure, sleep schedules, and eating patterns. However, misalignment between internal biological rhythms and social demands can compromise food choices, potentially leading to overweight and obesity. This research aimed to assess how a person’s chronotype links to social jet lag (SJL), which in turn would relate to their nutritional status and food consumption patterns as a university student. 617 students from a State University located in the State of Paraná, Brazil, completed a cross-sectional research study that collected sociodemographic information/anthropometrics by means of an online survey. It included self-reported height/weight data and dietary habits. The Munich Chronotype Questionnaire (MCTQ) was utilized to determine each participant’s chronotype classification and SJL calculation. Researchers found that nearly half of the students (49.3%) displayed an Intermediate Chronotype, which is associated with a diet that contained elements of the “Mixed” Diet, meaning there are equal portions of healthy food (Fresh Fruits, Beans, etc.) and unhealthy foods (Sweetened Beverages). The multivariate logistic regression analyses identified age as a significant predictor of obesity risk (OR: 1.15, p < 0.001), while dietary habits such as fruit consumption played a protective role. Additionally, having a breakfast protected them from being classified as obese compared to those who did not eat breakfast (OR = 0.59). Contrary to expectations, late-night supper was not a statistically significant predictor in the adjusted model. Predictors of an Intermediate chronotype included being male and eating morning snacks regularly. The results of this study suggest that students with an intermediate chronotype will predictably have skewed eating patterns, such as skipping breakfast and eating late—both of which affect obesity risks. Nutritional strategies for university students should focus on promoting circadian regularity and optimizing meal timing. Full article

Background: Our growing understanding of the brain basis of mind has seen an interest in evolutionarily ancient structures, most notably the brainstem. This paper offers an interesting example of this underexplored territory, by considering the mesencephalic component of the trigeminal nucleus. This largely uncelebrated brainstem structure is central to control of the jaw, and for the foundational acts of eating, oral exploration, and biting. Objectives: This paper explores the interesting anatomy of the mesencephalic trigeminal: unique in the nervous system as a centrally located sensory ganglion, which combines sensory and motor function for the jaw. An unexplored aspect of its anatomy is that the mesencephalic component of the nucleus lies directly adjacent to the brain’s core system for the experience of emotion, the peri-acqueductal gray (PAG). Results: The data suggest a role for the jaw, and more broadly the oral cavity, in relation to a range of feeling states, from pleasure to aggression. This is supported by behavioural and classic neuropsychological findings, such as the Klüver-Bucy syndrome. However, the proposal is not well-supported by findings of direct connections between the trigeminal nucleus and the PAG. Conclusions: While these contrasting findings present a conundrum, there may be a role for non-synaptic signalling, of the sort increasingly understood to be important for interoception and homeostasis. Full article

The monitoring of wildlife habitats is crucial for effective conservation efforts, particularly where biodiversity faces significant threats. This study aimed to monitor the biodiversity of wild mammals in a protected area (PA) of Northeastern Bangladesh, with a particular focus on detecting previously unrecorded species using camera traps. We deployed nine camera traps across 19 locations inside the PAs of Satchari National Park (SNP) from May 2024 to April 2025. Further, the camera-trap data were analyzed to evaluate the existing wild mammals, along with their activity patterns and seasonal variations, in SNP. Our study identified the Asiatic black bear in SNP for the first time, representing a significant contribution to biodiversity records of Bangladesh. Among the other frequently documented wild mammals were the wild boar, northern pig-tailed macaque, and barking deer, whereas less commonly detected species included the crab-eating mongoose and jungle cat. Activity pattern analysis of Asiatic black bear revealed a predominantly nocturnal-to-crepuscular behavior, with distinct bimodal peaks during early morning and evening. The present study showed that the Asiatic black bear was active in pre-monsoon and winter; however, it was absent during the rainy season, suggesting seasonal habitat use or detectability challenges. This is the first study to confirm the presence of Asiatic black bears in PAs of SNP using camera traps. These findings also highlight the importance of long-term biodiversity monitoring for continued conservation efforts to protect the diverse wildlife of SNP. The detection of previously undocumented wild mammals highlights the ecological importance of SNP, urging authorities to tighten the ongoing conservation initiatives. Understanding the diel and seasonal activity patterns would instruct the timing of conservation and habitat management strategies. This study also makes the integration of camera-trap monitoring into long-term biodiversity management to guide evidence-based conservation policies in Bangladesh’s PAs. Full article

Considering sustainable consumption not just as an exercise of individual choice but a shared and collective activity, this study explores the role of conscious and responsible consumption initiatives (CRCIs) driving citizens’ adoption of sustainable lifestyles. This research followed a qualitative approach, combining documentary research and twenty-six in-depth interviews with practitioners in eight grassroots consumer initiatives located in Galicia (Spain). The results show that CRCIs favor members’ consumption of organic, seasonal, fair, and locally produced food. The findings also reveal that engagement in these initiatives nurtures three interconnected types of learning—cognitive, attitudinal, and behavioral—which contribute to wider adoption of sustainable practices related to shifts in dietary habits, energy use, mobility, and frugality. CRCIs facilitate gradual transitions toward reduced meat consumption, favoring the intake of plant-based foods, and greater self-efficacy in preparing sustainable meals. These behavioral changes are incremental, motivated by inner reflection, practical experience, and consciousness around alternative economic models. However, the consistent adoption of sustainable eating habits is hindered by cultural and psychological barriers like cultural traditions, entrenched habits, and time constraints. In conclusion, these grassroots initiatives are interesting entry points for engaging citizens in sustainable lifestyles, becoming also gateways to the broader social and solidarity economy movement. Full article

Background: Anemia remains an important public health problem worldwide. Investigating the potential influencing factors of anemia can provide a reference for improving anemia status. This study aimed to identify factors influencing anemia in school-age children and assess associations/causal relationships between micronutrients (vitamin A, vitamin D, iron, and zinc) and anemia risk. Methods: This study included 1725 school-age children. Factors associated with anemia were identified using multivariable-adjusted logistic regression. Associations of serum micronutrients with anemia were analyzed, and non-linear relationships were examined. Causality was assessed using two-sample Mendelian randomization (MR) analysis. Results: Daytime outdoor activity, milk consumption, school location, picky eating, and serum ferritin deficiency were associated with anemia (p < 0.05). Higher serum vitamin A (Q4 vs. Q1: OR = 0.548; P_trend = 0.027) and higher serum ferritin (Q4 vs. Q1: OR = 0.470; Q3 vs. Q1: OR = 0.609; P_trend = 0.011) were inversely associated with anemia. RCS indicated a J-shaped non-linear relationship between serum ferritin and anemia risk. MR analysis showed that serum 25-hydroxyvitamin D (OR = 0.864, 95%CI: 0.757–0.986, p = 0.030), serum ferritin (OR = 0.656, 95%CI: 0.588–0.731, p < 0.001), and serum iron (OR = 0.793, 95%CI: 0.681–0.925, p = 0.003) significantly reduced anemia risk with the IVW method. Sensitivity analyses showed no heterogeneity, pleiotropy, or reverse causality. Conclusions: This study found that daytime outdoor activity time, weekly milk consumption frequency, school location, picky eating, and serum ferritin deficiency are closely associated with anemia in school-aged children. Additionally, serum vitamin A, vitamin D, serum iron, and serum ferritin levels are also linked to anemia. These findings collectively highlight the importance of lifestyle factors and specific micronutrients in influencing anemia among school-aged children, providing valuable insights for targeted prevention and intervention strategies. Future intervention trials focusing on these key factors could further validate their practical application value. Full article

Background/Objectives: Dietary microstructure affects energy intake. Traditional Universal Eating Monitors (UEMs) offer accuracy but are limited for monitoring diverse diets. We developed the ‘Feeding Table’, a novel UEM that simultaneously tracks intake of up to 12 foods, enabling high-resolution monitoring of eating microstructure for multiple foods simultaneously. Methods: Forty-nine healthy volunteers participated: 15 (10 male, 8 female) in a location preference experiment and 31 (15 male, 16 female) in a standard meal test. The location preference study involved four weekly sessions. Participants received a standardized breakfast based on individual energy needs; lunch intake was measured 3 h later with food items in pseudo-randomized positions. The standard meal test occurred over two consecutive days to assess the Feeding Table’s performance in monitoring eating behavior under standardized conditions. Results: In two consecutive days of standard meal tests, the Feeding Table showed reasonable day-to-day repeatability for energy and macronutrient intake (energy: r = 0.82; fat: r = 0.86; carbohydrate: r = 0.86; protein: r = 0.58). Among the four repeated intake measurements, the results demonstrated high intra-class correlation coefficients (ICCs: energy 0.94, protein 0.90, fat 0.90, and carbohydrate 0.93). No significant positional bias was observed (energy: p = 0.07; macronutrients: p = 0.70). Conclusions: The Feeding Table maintains UEM accuracy while enabling multi-food, real-time monitoring of dietary microstructure and food choice, offering enhanced precision for studying eating behaviors. Full article

Background/Objectives: The prevalence of dental caries in Romania is significantly high, especially among children and adolescents. We aimed to assess the prevalence of dental caries and their associated factors among schoolchildren aged 6–11 years learning at urban and rural schools from Mureş County, Romania. Methods: This cross-sectional study included a sample of 1124 children, aged 6–8 years (n = 524), as well as aged 9–11 years (n = 600). Nine schools in Mureş County, Romania, were selected for screening, based on their location (4 schools from urban areas and 5 schools from rural areas). Data were collected based on children’s visual dental screenings and a self-administered questionnaire addressed to their parents to collect information about oral health behaviors, sugar consumption, and dental care history. Dental clinical examination was performed by specialists, and DMFT/dmft values were recorded. Binary logistic and negative binomial regression analyses were used to assess the factors associated with dental caries. Results: Among 6–8-year-olds, the prevalence of untreated decay was 76.5% and the prevalence of caries experience was 77.7% (mean dmft = 3.9). Among 9–11-year-olds, the prevalence of untreated decay was 43.5% and the prevalence of caries experience was 48.2% (mean DMFT = 1.9). Among the most significant factors associated with caries prevalence were school location (p = 0.04 for children aged 6–8 years, and p < 0.001 for 9–11 years); the employment status of mothers (p = 0.04 for 9–11 years); eating sweets ≥4 times/day (p = 0.04 for 6–8 years); brushing time ≥3 min (p = 0.03 for 9–11 years); as well as past dental restorative treatments or emergency (p < 0.001 for all the children examined). Conclusions: Preventive measures and innovative educational interventions are needed to mitigate the impact of dental caries prevalence on the health and education of schoolchildren. Full article

Listeria monocytogenes is a foodborne pathogen capable of contaminating ready-to-eat meat products, e.g., frankfurters. Post-packaging mild heat treatment via water immersion is commonly employed; however, this may be sublethal to cells located in protected niches or beneath the product surface. The objectives of this study were to evaluate thermal injury of L. monocytogenes on frankfurters at single-cell versus population level and to comparatively estimate pathogens’ physiological status. Pork frankfurter-type sausages were inoculated (ca. 7.0–7.5 log CFU/cm²) with L. monocytogenes strain EGDE-e. Heat treatment was performed at 61 °C (max. 60 min) and 64 °C (max. 12 min). To determine the injured subpopulation from the total, tryptic soy agar with 0.6% yeast extract (TSAYE), supplemented or not with 5% NaCl, was used. Plating-based quantification of injured cells was compared to CFDA/PIstained cells analysed by fluorescence microscopy and quantified with Fiji software. Injury was recorded mainly after 2 and 4 min at 64 °C, whereas no injury was detected at 61 °C, at population level. Following exposure to 61 °C for 60 min, culturable cells dropped below the enumeration limit (0.3 log CFU/cm²), while a considerable number of CFDA⁺/PI⁻ and CFDA⁺/PI⁺ cells indicated viable-but-non-culturable induction and sublethal injury, respectively. These findings suggest that non-culturability may limit the accuracy of solely culture-based enumeration methods. Full article

Obesity has emerged as one of the most significant public health challenges of the 21st century, with its prevalence increasing at an alarming rate globally. While individual factors such as diet and physical inactivity are well-known contributors, the built environment, particularly housing, plays a critical yet understudied role in shaping obesity-related behaviors. This study examines the multilayered relationship between housing and obesity, focusing on built and neighborhood environment, affordability, and the social environment. Poor housing quality, such as overcrowding and inadequate ventilation, can potentially lead to chronic stress and sedentary behaviors, while housing design influences physical activity through characteristics such as design features and outdoor spaces. Housing location affects access to amenities such as parks and healthy food options, with disparities in access contributing to obesity in low-income areas. Similarly, neighborhood walkability, influenced by infrastructure and land use, encourages active transportation and recreation. Housing affordability also impacts dietary choices and access to recreational facilities, particularly for low-income families. Moreover, the social environment within housing communities can foster or hinder healthy behaviors through social networks and community engagement. This study emphasizes the need for health-conscious urban planning and policies that address these housing-related factors to combat obesity and promote healthier lifestyles. By integrating these Obesity–Housing Nexus, policymakers can create environments that support physical activity, healthy eating, as well as overall health and well-being. Full article

Background: Emotional eating, defined as the tendency to eat in response to emotions, has been associated with various biopsychosocial factors. However, in the Peruvian context, there is limited evidence regarding the specific predictors of this eating behavior in adolescents. Objective: To examine the associations between saturated fat intake, sleep duration, body mass index (BMI), depressive symptoms, age and sex, and emotional eating in Peruvian adolescents. Methods: This was a predictive cross-sectional study based on non-probabilistic sampling. A total of 722 adolescents from four schools located in East Lima were included. A multiple linear regression model was employed to examine the relationships between age, sex, saturated fat intake (SFI), sleep duration, BMI, and depressive symptoms and emotional eating. Results: The model was statistically significant (adjusted R² = 0.301; F = 45.276; p < 0.001), explaining 30.1% of the variance in emotional eating based on the explanatory variables. Being female (β = 0.208; p = 0.011), aged 15 to 18 versus 12 to 14 years (β = 0.083; p < 0.001), having a high SFI (β = 0.186; p < 0.001), sleeping ≥ 7 h (β = −0.126; p < 0.001), and a higher BMI (β = −0.082; p = 0.011) were significantly associated with emotional eating. Depressive symptoms (β = 0.365; p < 0.001) emerged as the strongest predictor in the model. Conclusions: Emotional eating among Peruvian adolescents is associated with psychological, behavioral, and sociodemographic factors. Depressive symptoms showed the strongest association, while longer sleep duration was linked to lower emotional eating scores. These findings highlight the need for integrated interventions targeting mental health, sleep hygiene, and healthy dietary behaviors in adolescents. Full article

The most frequently used laboratory animals for studies on adipose tissue properties and obesity are rodents. However, there are significant differences in the types of visceral fat depots between rodents and humans, including fat depots in the heart area. The large human fat depot of greatest interest in cardiac research is the epicardial adipose tissue (EAT). Its properties are widely investigated, because the EAT lies directly on the heart’s surface and can easily affect myocardial physiology. The major fat depot in rodents‘ chest—pericardial fat—is located on the ventral surface of the parietal lamina of the pericardium and is often incorrectly referred to as the EAT. Further confusion arises from reports claiming that rodents are entirely devoid of the EAT. We decided to verify adipose tissues in the heart area of 16 male Sprague Dawley rats under physiological conditions and in obesity. The animals in the NFD group (n = 8) were fed with a standard diet while these in the HFD group (n = 8) were fed with a high-fat diet (31% fat) starting from 4 weeks after birth. When the animals reached 12 weeks, the presence of fat deposits was verified. Additionally, their blood was collected to characterize carbohydrate and lipid metabolism changes, adipokine profile alterations, and their systemic inflammation status. The obesogenic diet caused significant disturbances in their carbohydrate and lipid metabolism, as well as hyperleptinemia. A high-fat diet primarily promoted the accumulation of pericardial fat, which was absent in the NFD rats and observed in 6 out of the 8 HFD animals. In both groups, adipocytes were also found directly on the hearts’ surfaces (EAT), albeit in very small numbers and limited to the atrioventricular groove on the dorsal side of the hearts. These adipocytes were dispersed among the vessels, making quantitative assessment and separation difficult, however, macroscopic evaluation revealed no noticeable differences in its extent. In conclusion, although rats are not entirely devoid of the EAT, their suitability for studying the properties of the EAT appears to be considerably limited. Full article

Background: A national study from China in 2023 predicted that the prevalence of overweight and obesity among children aged 7–18 will increase from 23.4% in 2019 to 32.7% by 2030. Objectives: This study aimed to investigate the longitudinal association between parental feeding practices and children’s body composition and weight status, and to assess the mediation effect of parental feeding practices in the relationship between socioeconomic status and childhood body composition. Methods: This longitudinal observational study was conducted between September and November 2021 in eight primary schools located in Luohu District, Shenzhen. Baseline and two follow-up surveys were administered annually during the same period from 2021 to 2023 (with one-year intervals). A total of 620 third-grade students (aged 8–10 years at baseline) and their parents were ultimately included in the study. Associations between parental feeding practices and children’s weight status and body composition were analyzed using mixed-effects models. The mediation effect of parental feeding practices on the relationship between socioeconomic status and childhood body composition was assessed through bootstrapping analysis. Results: At follow-up, a significant upward trend in the prevalence of central obesity among children was observed. Among all parental feeding dimensions, perceived child weight (PCW) demonstrated a strong association with central obesity after Bonferroni correction (OR = 1.33, 95% CI = 1.16, 1.51); similarly, monitoring (MN) and concern about child weight (CN) were both significantly associated with central obesity as risk factors for central obesity (OR = 1.14, 95% CI = 1.06, 1.23; OR = 1.16, 95% CI = 1.07, 1.27), both p < 0.001. These associations were modified by baseline child sex, parental BMI, and maternal and paternal education levels. However, restriction (RST) was not significantly associated with either body composition or weight status. The relationship between family socioeconomic status (SES) and childhood overweight and obesity was mediated by pressure to eat (PE) (p < 0.05). Conclusions: MN, PCW, and CN are associated with an increased risk of obesity in children. However, no significant association was found between parental feeding practices and changes in children’s body composition. Full article

The main goal of this study was to investigate and better understand the evolution of the main non-destructive and destructive quality parameters of peach ‘Redhaven’ during ripening process. This study was conducted from 8 to 21 July 2023, during which peaches ‘Redhaven’ were harvested each second day from a commercial orchard located in Novaki Bistranjski. Maturity categories were defined according to different firmness thresholds: maturity for long-distance chain stores (H1), maturity for medium-distance chain stores (H2), maturity below the defined maximum firmness in order to preserve optimal quality traits (H3), ready to buy (H4), ready to eat (H5), and overripe (H6). The chlorophyll absorbance index was the non-destructive parameter that was mostly distinguished between maturity categories (r = 0.78 with firmness), followed by a* and h° ground colour parameters. During the first three maturity categories (H1–H3), firmness had a notably smaller correlation with titratable acidity and the ratio of total soluble solids and titratable acidity, which is not the case for a* and h° ground colour parameters, chlorophyll absorbance index, and the share of additional colour. During the last three maturity categories (H4–H6), non-destructive parameters are not reliable for maturity prediction. When ground colour parameters are measured near petiole insertion, mostly smaller segregation between maturity categories is obtained compared to when measured at the rest of the fruit. Total polyphenol and flavonoid content in peach juice notably corelated only in the last two maturity categories with L* ground colour parameter. Full article

Understanding the economic value of natural resources is essential for the sustainable management of national parks. However, most existing studies focus on single conservation objectives and overlook the valuation of multiple resources within the same ecosystem. This study estimates tourists’ willingness to pay (WTP) for the conservation of four natural resources—crab-eating macaques, coral reefs, dry evergreen forests, and clean air—within Khao Laem Ya-Mu Ko Samet National Park in Thailand. Using the contingent valuation method and seemingly unrelated regression (SUR) analysis, data were collected from 205 tourists using structured surveys. The results show that dry evergreen forests received the highest average WTP (THB 129/year), followed by coral reefs (THB 125), clean air (THB 110), and crab-eating macaques (THB 107). At the population level, aggregate annual WTP values ranged from THB 85.7 million to THB 103.2 million across the resources. Age and awareness levels were consistent positive predictors of WTP, while other influencing factors—such as education, income, nationality, and residential location—varied by resource type. The findings provide empirical insights for designing resource-specific conservation strategies and highlight the potential of economic valuation to support sustainable financing mechanisms and policy development for protected area management. Full article

The behavioral changes of dairy cows directly reflect their health status, and observing the behavioral changes of dairy cows can provide a scientific basis for dairy farms so managers can take timely measures to intervene and effectively prevent diseases. Because of the complex background, multi-scale behavior changes of dairy cows, similar behavior, and difficulty in detecting small targets in the actual dairy farm environment, this study proposes a dairy cow behavior recognition algorithm, DMSF-YOLO, based on dynamic mechanism and multi-scale feature fusion, which can quickly and accurately identify the lying, standing, walking, eating, drinking and mounting behaviors of dairy cows. For the problem in multi-scale behavior changes of dairy cows, a multi-scale convolution module (MSFConv) is designed, and some C3k2 modules of the backbone network and neck network are replaced with MSFConv, which can extract cow behavior information of different scales and perform multi-scale feature fusion. Secondly, the C2BRA multi-scale feature extraction module is designed to replace the C2PSA module, which can dynamically select the important areas according to the two-layer routing attention mechanism to extract feature information at different scales and enhance the multi-scale feature extraction capability of the model, and the same time inhibit the interference of the background information to improve the small target detection capability of the model. Finally, the Dynamic Head detection head is introduced to enhance the model’s scale, spatial location, and perception of different tasks, enhance the capacity to extract similar behavioral features of cows, and improve the model’s performance in detecting cow multi-scale behaviors in complex environments. The proposed DMSF-YOLO algorithm is experimentally validated on a self-constructed cow behavior dataset, and the experimental results show that the DMSF-YOLO model improves the precision (P), recall (R), mAP50, and F1 values by 2.4%, 3%, 1.6%, and 2.7%, respectively, and the FPS value is also high. The model can suppress the interference of background information, dynamically extract multi-scale features, perform feature fusion, distinguish similar behaviors of cows, enhance the capacity to detect small targets, and significantly improve the recognition accuracy and overall performance of the model. This model can satisfy the need to quickly and accurately identify cow behavior in actual dairy farm environments. Full article