Search Results (9,958)

Acinetobacter baumannii is widely recognized as a problematic pathogen in healthcare settings due to its ability to acquire resistance to multiple antimicrobial agents. However, less attention has been given to its presence outside hospitals. In this cross-sectional, laboratory-based surveillance study, we investigated the occurrence of A. baumannii in ready-to-eat (RTE) foods sold at retail outlets in four cities of the Al-Qassim region, Saudi Arabia, during a single season. A total of 240 RTE food samples were analyzed using culture-based and molecular approaches for species confirmation, and antimicrobial susceptibility profiles were determined. A. baumannii was identified in 19 samples (7.9%), spanning several food categories. Most isolates showed resistance to multiple antimicrobial classes, and 16 met the criteria for multidrug resistance (MDR). Among the confirmed isolates, bla_OXA-23-like was detected in 16 (84.2%), bla_{OXA-24/40-like} in 2 (10.5%), and bla_OXA-58-like in 1 (5.3%). Resistance to fluoroquinolones, tetracyclines, and aminoglycosides was common, and OXA-type carbapenemase genes were detected in 16 isolates. These findings indicate that RTE foods can represent non-clinical environments in which MDR A. baumannii may be detected. Including food sources in antimicrobial resistance surveillance may therefore strengthen our understanding of the ecology of this pathogen within a One Health framework. Full article

Edible insects, despite their high nutritional value, are widely rejected due to neophobia associated with eating them whole. This study evaluated crispy corn tortillas (tostadas) enriched with cricket flour (CF) and defatted cricket flour (DCF) to increase protein content and acceptability. The tostadas were prepared using CF and DCF as substitutes for corn flour (5–25%). Physicochemical evaluation was conducted using proximal analysis, color measurements, and pH measurements. Sensory evaluation included Check all that apply (CATA); general liking, Just about right (JAR); preference mapping; and penalty analysis. The results showed increases in protein, fat, and ash content, and decreases in moisture and carbohydrates. The samples with cricket flour exhibited b* values similar to the control, an increase in a*, and a decrease in L*. The most notable descriptors for the tostadas with cricket flour were herb flavor, brown color, and granular texture. The treatments containing 5% and 15% CF showed the highest liking scores, whereas the control sample presented the lowest level of acceptance. In this regard, the control sample reduced the overall mean liking score. Therefore, most consumers exhibited preference patterns toward the samples formulated with CF and DCF, which were associated with higher overall acceptability. The penalty analysis indicated that the samples should be saltier and darker. In conclusion, tortillas enriched with cricket flour increase protein content and acceptability, although the formulation could be further improved to enhance overall liking. Full article

Background/Objectives: Social vulnerability is linked to unhealthy eating habits, low physical activity, and, overall, increased health risks and low well-being. This study examined self-perceived barriers and facilitators to engaging in obesity prevention policies for children at risk of poverty and social exclusion in Greece from the perspective of parents, caregivers, and community representatives. Methods: A qualitative study was conducted from November to December 2023 in three geographically diverse Greek regions, namely Attica, Thessaly, and Crete. A qualitative study was conducted between November and December 2023 in three geographically diverse regions of Greece. In total, seventy-two individuals participated in the study through individual interviews and focus groups. Forty-five parents of children with disabilities took part in individual interviews, equally represented in all three regions (fifteen participants per region). Among focus group participants: Twenty-one caregivers from child protection units participated in six focus groups (two per region), with focus group sizes ranging from three to five participants. In addition, six Roma community representatives participated in three focus groups (one per region), with focus group sizes ranging from one to four participants. Inductive and deductive thematic analysis were performed using NVivo 14 software to identify key themes. Results: Most factors that increased engagement were perceived by participants as both barriers and facilitators. These factors were classified at the individual, sociocultural, or structural level, and similar themes emerged across groups. Common barriers to poor engagement included low health literacy, financial difficulties and underfunding, social exclusion, a lack of targeted nutrition interventions, concerns related to training opportunities and support, and the adequacy and safety of built environments. Common facilitators of enhanced engagement included increased awareness and motivation to support vulnerable children, the availability of community- and school-based initiatives, and free school meal provision. Conclusions: Engagement in obesity prevention policies targeting vulnerable children is influenced by multiple interrelated factors. Understanding these barriers and facilitators from the participants’ perspectives can guide policymakers and practitioners in designing more effective obesity-related interventions for socially vulnerable groups of children. Full article

Objectives: With the rapid development of digital technology, the risk of internet addiction among adolescents has increased. However, the influence mechanism of emotional eating behavior on internet addiction remains unclear. This study aimed to explore the association pathway of emotional eating on internet addiction in junior high school students and test the chain-mediating effects of sleep quality (sleep quality was measured using the PSQI, with higher scores indicating poorer sleep quality) and depression. Methods: Based on data from 3245 junior high school students in Shenzhen, China, internet addiction was measured using Young’s questionnaire, and emotional eating was assessed via the Dutch Eating Behavior Questionnaire’s subscale. The PROCESS macro (Model 6) was used to test the chain-mediating effects. Results: Emotional eating was positively but modestly associated with internet addiction (β = 0.024, p < 0.01). Three significant mediating pathways were identified: (1) emotional eating → sleep quality → internet addiction (β = 0.0062, 14.52% of total effect); (2) emotional eating → depression → internet addiction (β = 0.0084, 19.67%); and (3) emotional eating → sleep quality → depression → internet addiction (β = 0.0041, 9.60%). Conclusions: Based on cross-sectional data, this study found that emotional eating is associated with internet addiction through the independent and chain-mediating effects of sleep quality and depression, revealing a statistical mediation pathway of “maladaptive emotion regulation → circadian disruption → psychopathology → addictive behavior.” These findings provide a basis for interventions targeting sleep management and emotional regulation. Full article

Background/Objectives: Sweetness is a key determinant of the eating quality of sweet corn, primarily governed by the soluble sugar content in kernels. The soluble sugar content decreases rapidly during the postharvest shelf life, which directly affects the flavor and quality. Relatively few studies have been conducted on the shelf life of sweet corn. Methods: An F₆ recombinant inbred line (RIL) population was constructed from two super sweet inbred lines with contrasting soluble sugar degradation rates: D174 (low degradation rate) and D179 (high degradation rate). Extreme phenotype pools were established using soluble sugar content as the target trait. Based on bulked segregant analysis sequencing, we identified chromosomal segments associated with postharvest soluble sugar reduction in sweet corn, annotated the gene information within these segments, and analyzed the functions of the annotated genes using the Gene Ontology and Genomes databases. Results: Results revealed three associated regions located at 44,205,775–45,290,843 bp on chromosome 4, 6,250,656–6,744,665 bp on chromosome 2, and 135,428,709–136,732,132 bp on chromosome 10. This interval contained 195 genes. Integrated analysis of gene expression, gene annotations, and quantitative real-time PCR indicated that Zm00001eb069070, which is highly expressed in kernels with a prolonged shelf life, might be a key candidate gene regulating soluble sugar degradation in sweet corn. Conclusions: This study provides valuable genetic resources for the improvement of favorable agronomic traits and the advancement of molecular breeding strategies for sweet corn. Full article

The present study examines the behavioural trajectories and spatial utilisation of elderly caregivers within intergenerational families, set against the backdrop of China’s accelerating ageing population and the widespread prevalence of dual-income households. Existing studies predominantly rely on static data, which makes it difficult to capture the dynamic relationship between behaviour and space. The present study employs lagged sequence analysis in combination with non-participatory observation and video recording techniques to conduct a 14-day behavioural tracking and sequence analysis of two typical dual-income families in Beijing (totaling 2137 behavioural events), thereby establishing a research framework of “behavioural observation, sequence analysis, and design translation.” The identification of three typical behavioural sequence patterns was achieved through the implementation of behavioural coding, spatio-temporal trajectory modelling, and sequence correlation testing. The identified sequence patterns are as follows: a simple “cooking–eating” sequence, a complex “child-centred” sequence, and a cyclical “housework–rest–communication” sequence. These patterns exposed fundamental contradictions with prevailing spatial functions. The study proposes synergistic spatial and furniture design strategies to support elderly caregivers’ behavioural flow, alleviate caregiving burdens, and foster intergenerational integration. This research not only validates the methodological value of lag sequence analysis in behaviour-driven design but also provides theoretical and empirical foundations for sustainable residential environments that promote intergenerational cohesion and reduce caregiving stress. Full article

Slow-oscillation neurofeedback (NF), encompassing slow cortical potential (SCP), infra-low-frequency (ILF), and infra-slow-fluctuation (ISF) protocols, has gained increasing interest as a non-pharmacological intervention in pediatric mental health and neurodevelopmental care. This narrative review synthesizes peer-reviewed literature on the clinical efficacy of slow-oscillation NF in children and adolescents across various conditions, including attention-deficit/hyperactivity disorder (ADHD), autism spectrum disorder (ASD), epilepsy, tic disorders, and eating-related concerns. SCP NF is the most extensively studied protocol and shows preliminary efficacy in reducing ADHD symptoms, particularly among individuals capable of learning self-regulation. For ASD and other conditions, early evidence from primarily small-scale or uncontrolled studies suggests possible benefits in emotional regulation, impulsivity, and behavioral symptoms, though findings remain mixed and often non-specific. Methodological heterogeneity, including variation in control conditions, training protocols, and outcome measures, limits the comparability of results. ILF and ISF protocols, while promising, are still emerging and require further validation. Overall, slow-oscillation NF appears to offer potential as a personalized therapeutic option for pediatric populations, but robust, well-controlled trials are needed to clarify its clinical utility and optimize its integration into multimodal care. Full article

Background/Objectives: Research on children’s eating has primarily focused on parental feeding practices and dietary outcomes, with less attention to how young children themselves understand parental food-related messages and relate them to their own food choices. Recognizing children as active participants in food socialization, this study aimed to examine preschool children’s representations of parental feeding strategies alongside their expressed food-choice considerations. Methods: A qualitative, exploratory, multi-method design was employed within a constructivist framework. Forty kindergarten children aged 4 years 10 months to 5 years 8 months participated in individual, play-based sessions conducted in familiar educational settings. Data were generated using two complementary tools: a doll role-play task eliciting children’s representations of parental feeding strategies and a simulated grocery shopping task eliciting food-choice considerations. All sessions were audio-recorded, transcribed verbatim, and analyzed using reflexive thematic analysis. Results: During role-play, children frequently portrayed parents as emphasizing health-related arguments, control, and negotiation when guiding food intake. Less frequently, they represented strategies such as encouragement to try, deception, or references to body weight. In contrast, during the food-choice task, children’s selections were primarily guided by personal preference, with health considerations mentioned less often. For most participants, the feeding strategies attributed to parents did not closely align with the considerations guiding their own food choices. Conclusions: The findings highlight young children’s active and selective engagement with parental feeding discourse and underscore the contextual nature of food-related meaning-making in early childhood. Rather than reflecting a straightforward transmission of parental messages, children’s food choices appear shaped by situational affordances and perceived autonomy, supporting child-centered approaches to nutrition education and health promotion. Full article

Can kelp farming provide a low-impact food source within Arctic marine food systems? This study presents the first life cycle assessment of kelp production in Greenland, assessing environmental impacts from offshore cultivation and on-site freezing through export to Denmark for downstream processing into ready-to-eat fermented kelp-based food products, using empirically grounded operational data. Particular attention is given to discrepancies between expected and realised biomass yields. Results show that life cycle impacts per kilogram of wet harvested kelp are highly sensitive to realised yields, with climate change impacts increasing from 1.00 to 3.83 kg CO₂-eq kg⁻¹ under observed yield conditions. Offshore cultivation infrastructure and interregional transport dominate environmental burdens, while downstream processing contributes less. At the food-product level, four fermented kelp-based products are evaluated and compared with cabbage-based analogues. Kelp-based products exhibit lower land-use impacts but higher climate change and freshwater eutrophication impacts across multiple functional units. Additionally, kelp harvesting results in quantified removal of nitrogen and phosphorus from the marine environment. Overall, the findings indicate that kelp farming can represent an environmentally viable component of Arctic food systems, with yield stability and logistics identified as key determinants of sustainability. Full article

Background: Adults with inflammatory bowel disease (IBD) have a high prevalence of modifiable cardiometabolic risk factors. This study investigates the impact of a personalised diet and physical activity intervention versus usual care on the risk factors. Methods: A 6-month randomised controlled trial was conducted at three hospitals in New Zealand (NZ) from 2023 to 2024. Adults with IBD in remission, a body mass index >25 kg/m², and a low fibre intake <25 g/day were recruited. Participants were randomised to receive either generic healthy eating and physical activity education or personalised heart-healthy eating education based on the NZ Heart Foundation and a self-led physical activity program. The primary outcome was change in body fat, and secondary outcomes included disease activity, biomarkers, quality of life, physical activity, and dietary intake. Between-group differences were analysed using multivariable regression. Results: Sixty-four participants were randomised, and 51 (80%) completed the intervention. The median age was 47 years (LQ,UQ: 37, 55), 59% participants were female, 61% had Crohn’s disease, and 85% had faecal calprotectin <150 µg/g. Common cardiometabolic risks were high waist circumference (88%) and abnormal lipid profile (56%). There were no significant differences in primary or secondary outcomes except for dietary intakes: increased fruit (0.5 serves/day; 95% CI: 0.1, 1.0) and dietary fibre (3.1 g/1000 kcal/day; 95% CI: 1.1, 5.1); reduced discretionary food and drink (−1.7 serves/day; 95% CI: −3.0,−0.3), and sodium (−911 mg/day; 95% CI: −1783,−40). Conclusions: Personalised dietitian advice led to meaningful dietary changes without exacerbating disease activity. More intensive activity modalities can be recommended to support body composition improvements. Full article

Objective: Adolescence is a high-risk period for problematic eating behaviours, including overeating. However, few studies have explored adolescent perceptions of these eating behaviours and whether there is a shared understanding between adolescents and parents. This study aimed to investigate perceptions of eating behaviours, focusing on overeating, in Australian adolescents and parents. Method: Adolescents aged 13–19 years, and parents of adolescents, participated in two interviews for exploration and thematic deepening of participant perceptions, underpinned by Integrated Knowledge Translation Framework principles. Interviews explored perceptions of overeating and other eating behaviours, including help-seeking and stigma. Data were analysed thematically. Results: Twelve adolescents (59% female) and seven parents (100% female) participated in the interviews, with three major themes emerging. In theme 1, “perceptions of overeating”, interpretations of overeating varied; however, both adolescents and parents associated problematic overeating with increased frequency and impacts on functioning. Discrepancies between adolescent and parent perceptions of overeating terms such as binge eating were present. In theme 2, “beliefs about overeating”, adolescents felt that broaching the topic of overeating and help-seeking for overeating to be more challenging than restrictive eating disorders due to stigma. In theme 3, “perceptions of other eating behaviours”, there were differences between how adolescents perceived healthy eating and dieting compared to parents. Discussion: Differences in adolescent and parent understanding of eating behaviour terminology highlights a need for a shared language to support appropriate detection of problematic eating behaviours. There is a need for prevention and early intervention approaches that promote awareness and accessible support pathways for overeating to prevent progression to an eating disorder. Full article

Background/Objectives: This study examined relationships between diet quality, as determined using three a priori-defined dietary patterns (Healthy Eating Index of 2010 dietary guidelines [HEI-2010], Mediterranean Dietary Pattern [MDP], and Dietary Approaches to Stop Hypertension [DASH]), intestinal permeability, and features of the gut microbiota in a diverse, obese sample. Methods: This was a post hoc, cross-sectional study including 103 healthy, obese individuals (43.8 ± 11.3 years, BMI: 37.5 ± 6.1 kg/m², 64.1% African American). Dietary intake was assessed using the Vioscreen food frequency questionnaire. Intestinal permeability was assessed via urinary sugar excretion and microbiota features were characterized using 16S rRNA gene amplicon sequencing. Relationships between dietary pattern adherence, intestinal permeability, and gut microbiota were assessed using correlation coefficients and a general linear model. Results: Higher dietary pattern scores correlated with lower levels of intestinal permeability measures such as 24 h urinary sucralose (HEI-2010: r = −0.33, p = 0.002; MDP: r = −0.31, p = 0.004; DASH: r = −0.38, p < 0.0001) and 24 h sucralose-to-lactulose ratio (HEI-2010: r = −0.23, p = 0.03; MDP: r = −0.32, p = 0.003; DASH: r = −0.24, p = 0.03). Fruit intake consistently correlated with lower intestinal permeability measures (p < 0.05) across all three dietary patterns. Higher DASH scores correlated with lower Proteobacteria (r = −0.28, p = 0.004) and higher Verrucomicrobia (r = 0.30, p = 0.002) phylum abundance. Conclusions: The current results suggest a potential role for diet quality in promoting intestinal health. Full article

Background/Objectives: Chrononutrition is an emergent field concerning the effect of eating patterns on human health and their relationship with biological rhythms. Current evidence points towards the benefits of early eating in the prevention of non-communicable diseases and circadian health. Despite the importance of eating/fasting rhythm, current methods are neither specific nor validated against physiological variables. This work aimed to explore an objective metabolic outcome, postprandial glucose, as an accuracy indicator of self-declared meal schedules registered in a mobile app. Methods: A 1-week protocol of ambulatory monitoring of meal schedules, glucose, and circadian variables was performed in 20 young adults. Meal annotations were registered using KronoEat 1.0, a smartphone app, allowing for both prospective and recall entries. A circadian monitoring device provided data on movement intensity, distal skin temperature, and prospective food annotation. Results: Participants annotated an average of 3.5 food events/day/participant with KronoEat. Breakfast (92.7%) and lunch (86.4%) showed the highest proportion of food events related to a glycemic excursion, whereas this proportion was lower for dinner (79.7%) and snacks (67.7%). Postprandial glucose after main meals differed significantly from average glucose levels. Interesting couplings were found in circadian variables and glucose—for example, between post-breakfast glycemic excursions and the morning increase in activity. Conclusions: Meal schedules registered under free-living conditions in KronoEat show high levels of correlation with postprandial glucose and glycemic excursions derived from continuous glucose monitoring. Full article

Objective: Family-Based Treatment (FBT) is an emerging intervention for youth with low-weight Avoidant/Restrictive Food Intake Disorder (ARFID), yet little is known about how parents conceptualize progress, learning, and maintenance of behavioral changes after treatment completion. Clarifying parent perspectives may inform future treatment modifications and shed light on potential mechanisms of change in FBT. Methods: This qualitative study explored parent experiences (n = 19 families) during the final session of FBT-ARFID who were treated in the context of a randomized clinical trial. Qualitative data from final-session transcripts were analyzed using reflexive thematic analysis. Results: Qualitative analyses identified four themes capturing parental reflections on learning during FBT: Progress Review, Parent Learning, Maintenance Planning, and Gratitude. Parents emphasized improvements beyond weight restoration, including increased dietary variety, reduced fear around eating, and greater flexibility at meals. Parents universally reported learning core FBT principles and increased confidence about their ability to manage ARFID in their child after treatment was completed. Conclusions: According to systematic qualitative analysis of parent reflections at the end of treatment, FBT for ARFID promotes parental self-efficacy, multidimensional progress, and meaningful parental learning related to managing ARFID symptoms in their children. Full article

With global economic development and rising living standards, expectations regarding the quality of staple rice have become increasingly multifaceted. This shift has imposed more stringent demands on high-quality rice breeding and field management and has stimulated research into the mechanisms underlying changes in rice quality. This article explores how assessments of rice quality have evolved from a primary emphasis on appearance, eating and processing quality to include stronger requirements for nutritional value and safety. In rice production systems, quality outcomes are influenced by interactions among genetic traits, ecological factors and field management practices. Through genetic improvement, biological breeding techniques and precise field management, improvements in appearance, eating and nutritional qualities can be achieved. Although climate change is an uncontrollable external factor affecting rice quality, constructing multi-factor dynamic simulation models that target key genes has been proposed as a strategy to enhance stress resistance and guide rice breeding. Rice safety and quality depend on the rational use of pesticides in terms of timing and dosage, which can help mitigate disease and insect resistance while reducing the risks associated with pesticide residues and toxins. Furthermore, the application of artificial intelligence technologies in biological breeding and field management can shorten breeding cycles, improve disease and pest outbreak prediction and support the timely formulation of treatment prescriptions. Full article