Search Results (2,839)

Feed intake and diet quality are key factors influencing enteric methane (CH₄) emissions in ruminants. Low-quality C₄ grasses typically limit intake and are associated with high CH₄ yield. Nitrogen supplementation may improve rumen function and reduce CH₄ emissions per unit of feed intake, although responses under low-quality forage conditions remain insufficiently characterized. The goal of the study was to evaluate the effects of nitrogen supplementation (urea- or nitrate-containing supplements) on the utilization of low-quality weeping lovegrass hay (Eragrostis curvula) and CH₄ yield in beef steers. Twenty-four Aberdeen Angus steers (326 ± 27 kg body weight) were assigned to three treatments: (1) weeping lovegrass hay alone; (2) weeping lovegrass hay + sunflower expeller + urea; and (3) weeping lovegrass hay + sunflower expeller + potassium nitrate (KNO₃). The proportion of non-protein nitrogen (NPN; urea and KNO₃) included in the supplements was set according to the maximum tolerated threshold. Methane emissions were measured using the SF₆ tracer technique. Compared with the hay-only treatment, supplemented animals increased dry matter intake (DMI) by 35% and 38% in the urea and nitrate treatments, respectively (p < 0.01). Total CH₄ emissions (g/d) were not affected by treatment (p = 0.16). However, CH₄ yield (g CH₄/kg DMI) decreased by 27% and 38% in the urea and nitrate treatments, respectively (p < 0.01). The methane conversion factor (Ym) was also reduced in supplemented animals. Under the conditions of this study, supplementation of low-quality weeping lovegrass hay with nitrogen-containing supplements increased feed intake and reduced CH₄ yield without affecting total CH₄ emissions. These findings highlight the importance of considering CH₄ emission intensity, in addition to absolute emissions, when evaluating mitigation opportunities in forage-based beef production systems. Full article

Abiotic stresses, such as drought, salinity, and aluminum toxicity (Al³⁺), affect the growth and initial establishment of wheat plants, limiting crop yield in restrictive growing environments. Therefore, the early selection of tolerant genotypes adapted to multiple production environments is essential to optimize wheat production. A laboratory experiment was conducted to identify and recommend wheat cultivars that simultaneously combine adaptability and stability for initial morphological responses when subjected to stressful environmental conditions. Plants from 12 wheat cultivars were grown under non-stressful (control) and stressful conditions (drought, salinity and Al³⁺ stress), using a 4 × 12 factorial arrangement with four replicates. On the 28th day, the emergence rate, length, dry matter and vigor of the plants were measured. Abiotic stresses limit the initial growth and vigor of wheat plants, with drought causing the greatest limitation for plant growth and biomass accumulation, while salinity had the greatest impact on plant vigor indices. Aluminum toxicity limits root development and biomass allocation. Principal component analysis explained 67.76% of the total variability and distinguished the plant growing environments. The multi-trait index proved effective in cultivar selection, highlighting the cv. ORS Feroz due to its proximity to the ideotype and adaptability to multiple abiotic stresses. Full article

Sweet potato (Ipomoea batatas (L.) Lam.) aerial biomass has potential as an alternative forage resource for ruminants in semi-arid regions; however, the fermentative behavior of different genotypes remains poorly understood. This study evaluated the fermentation profile, nutrient losses, and chemical composition of silages produced from the aerial parts of ten sweet potato accessions cultivated under agroecological conditions. Wilted biomass from each accession was pooled, homogenized, and ensiled in four mini-silos used as subsamples for fermentation characterization. Hierarchical clustering identified two distinct groups, indicating clear genotype-dependent variation in silage performance. Accessions BGH-UNIVASF 8 and 16 showed superior fermentation efficiency, characterized by greater dry matter recovery, lower effluent and gas losses, and more stable fermentation profiles. In contrast, several high-yielding accessions exhibited greater fermentation losses, indicating a trade-off between biomass productivity and preservation efficiency. Total digestible nutrients varied among accessions but were not consistently associated with fermentation quality. Overall, the results demonstrate that silage quality in sweet potato is strongly genotype-dependent and highlight the importance of integrating agronomic, nutritional, and fermentative traits when selecting accessions for silage production under semi-arid conditions. Full article

As the Italian hop industry undergoes consolidation, assessing the environmental pressure of diverse cultivation and processing models is essential for sustainable growth. This study characterizes the Product Environmental Footprint (PEF) of Italian hop production through a multi-case analysis of eight representative farms. A primary data collection tool was utilized to quantify resource inputs, including water management, nutritional strategies, and phytosanitary defense. Following a rigorous thermodynamic consistency screening of the field data to eliminate unrepresentative parameters, the life cycle inventory focused on two validated regional anchor cases. The findings reveal a high degree of management heterogeneity, with dry cone yields ranging from 400 to 1673 kg of dry matter per hectare. Two functional units were defined: 1 kg of fresh hop cones (FU1) to assess cultivation impacts, and 1 kg of processed products (FU2) at the brewery gate to evaluate the full supply chain. Integrating deterministic life cycle impact outputs with a probabilistic Monte Carlo uncertainty analysis, the results indicate that the environmental impact varies significantly across commercial formats: Cryogenic Powder (2.33 ± 0.34 mPt/kg) represents the most resource-intensive format, while Raw Bales and T90 Pellets from high-yield models exhibit scores as low as 1.36 and 1.55 mPt/kg, respectively. The study identifies the agricultural phase as the primary environmental hotspot, driven predominantly by water deprivation. To address these burdens, a Sustainable Italian Hop (SIH) integrated scenario was developed. By combining precision irrigation, thermal decarbonization via biomass valorization, and a direct-to-pellet processing flow, this model achieved a 70% total reduction in the environmental footprint score (0.465 ± 0.076 mPt/kg) and an 86% reduction in water use impacts. Finally, the socio-technical and financial barriers to implementing the SIH framework are qualitatively evaluated. These results provide actionable benchmarks for aligning the emerging Italian hop supply chain with European Union climate neutrality objectives. Full article

Horticultural intensification in Mediterranean areas has increased the risk of nitrate pollution due to inefficient irrigation and nitrogen fertilisation management. The availability of simulation models aimed at rational nitrogen management in outdoor crops is limited. The objective of this study is to adapt the VegSyst model, initially developed for greenhouse vegetables, for use in open-field conditions in relevant crops, such as processing peppers and broccoli in Extremadura. VegSyst simulates dry matter production and nitrogen uptake by incorporating the influence of evaporative demand (TUE approach) in addition to the effect of radiation (RUE approach). Experimental field data obtained in five campaigns (peppers: 2020–2022; broccoli: 2020 and 2022) under different nitrogen doses were used. The model was calibrated, and critical N dilution curves were developed for each crop. Subsequently, the simulation of fi-PAR, dry matter production (DMP) and N uptake was validated using statistical indices (RMSE, RE, d, EF) and regression analysis. The model showed a high predictive capacity for N uptake in both crops, with values of d ≥ 0.98 and EF ≥ 0.90 in the validation campaigns. The fi-PAR simulation was acceptable in peppers and excellent in broccoli. In contrast, the DMP prediction showed notable deviations in peppers, especially in 2022, attributable to interannual variations in weather conditions and physiological limitations not considered by the model. In both crops, the TUE-based strategy was a better fit for the measurements than the RUE-based strategy, indicating that under semi-arid Mediterranean conditions, transpiration is the limiting factor for biomass production. The adaptation of the VegSyst-Outdoors model proved to be robust for simulating N uptake and sufficiently accurate to be integrated into decision support tools aimed at efficient fertilisation and irrigation management. Full article

The seedling production stage of lettuce (Lactuca sativa L.) is crucial for crop success, as it determines the initial quality of the plants. The use of seeds with rapid and uniform germination is essential to ensure proper seedling establishment. Among sustainable alternatives for water management, irrigation with ozonated water stands out due to its disinfectant potential and its ability to stimulate plant physiology. This study evaluated the effects of irrigation with ozonated water on the production and development of lettuce seedlings. The experiment was conducted in a completely randomized design (CRD) arranged in a 2 × 2 factorial scheme, with four replications. Two lettuce cultivars were tested: one with smooth leaves and another with crisp leaves. The variables analyzed included germination parameters (final percentage, germination index and mean germination rate, uniformity, and time to reach 10, 50, and 90% germination), as well as initial growth parameters (total height, shoot and root height, and dry matter content). Analyses were performed on 20 seedlings per cultivar. Irrigation with ozonated water promoted significant growth (p < 0.05) of the shoot and root growth, with increases of 16.90 and 4.99% for the smooth-leaf cultivar, and 24.27 and 9.26% for the crisp-leaf cultivar, compared to the control. Ozone application did not alter the microbiological, physical, or chemical parameters of the water. These growth-promoting effects are likely associated with increased oxygenation of the root zone, enhanced oxidation of organic matter in the substrate, and improved nutrient availability promoted by ozone-derived radicals, which may also optimise root respiration and reduce pathogenic pressure. The applied concentration of 5 mg L⁻¹ O₃ over a 25-day seedling production cycle proved effective and did not cause phytotoxic effects. Irrigation with ozonated water is an efficient and environmentally safe alternative for producing vigorous lettuce seedlings. Full article

This study evaluated the effects of dietary inclusion of free and protected acid protease on productive performance, milk composition, metabolic profile, nutrient digestibility, and ruminal environment in lactating Jersey cows. Fifteen multiparous cows (67 ± 7.5 days in milk; 27.5 ± 3.5 kg/day) were assigned to a 3 × 3 Latin square (5 squares) design with 21-day periods. Treatments consisted of: control (no enzyme), free protease (4.4 g/day), and protected protease (4.4 g/day). The protected form was developed using alginate-based encapsulation to enhance enzyme stability under ruminal conditions. Protease inclusion did not affect dry matter intake, milk yield, or feed efficiency (p > 0.05). However, free protease increased lactation persistency (p = 0.05) and improved fat-corrected and energy-corrected milk yields (p ≤ 0.02), with intermediate responses observed for protected protease. Milk fat and protein contents were higher in enzyme-fed cows (p ≤ 0.05), while other compositional parameters remained unchanged. Apparent crude protein digestibility was greater in cows receiving free protease (p = 0.037), with no effects on dry matter or fiber digestibility. Protease intake increased total volatile fatty acid concentrations and major fermentation products (acetate, propionate, and butyrate; p ≤ 0.01), indicating enhanced ruminal fermentation. Blood metabolites showed increased total protein and globulin levels in cows fed free protease (p ≤ 0.05), suggesting improved protein metabolism. Microbiota analysis revealed no differences in alpha or beta diversity; however, specific microbial taxa and predicted metabolic pathways were modulated by treatments, particularly in post-ruminal compartments. In conclusion, exogenous protease, especially in free form, improved protein utilization and corrected milk production without disrupting microbial stability. These findings highlight the potential of protease as a nutritional strategy to enhance efficiency in dairy systems through targeted modulation of ruminal function and nutrient metabolism. Full article

Red clover (Trifolium pratense L.) is an important forage legume that is also a valuable source of bioactive compounds with potential health-promoting properties. This study evaluated the variability among diploid (2n) and tetraploid (4n) red clover cultivars in forage productivity, quality-related parameters, polyphenol and flavonoid content, and antioxidant activity, in order to identify promising ideotypes for dual-purpose breeding. A total of 90 cultivars were assessed under field conditions; green matter yield, dry matter yield, crude protein content, and protein yield were analyzed together with total polyphenols, total flavonoids, and antioxidant activity. Spearman correlation and principal component analysis (PCA) were used to relate the traits and identify cultivars with contrasting characteristics. Cultivar differentiation was pronounced within each ploidy group, whereas diploid and tetraploid cultivars overlapped substantially in the multivariate space, indicating that ploidy alone is not a reliable predictor of forage or medicinal value. At the group level, tetraploids tended toward higher biomass, protein-related traits, and total polyphenol concentration, while total flavonoids and antioxidant activity were broadly comparable between groups. Forage- and medicinal-related traits were only weakly correlated and thus behaved as largely independent selection targets—which is precisely why integrated multi-trait evaluation is required to identify cultivars combining both. Several cultivars did combine favorable agronomic and phytochemical characteristics, supporting within-group selection of red clover germplasm with dual forage and medicinal potential for sustainable agricultural systems. Full article

Grass–legume mixtures can improve forage productivity and quality, but their performance may vary between years. This study evaluated grass–legume mixture types differing in composition and intended use (forage, universal, and grazing) across two growing seasons, focusing on the first cut. Mixtures were assessed for dry matter yield, botanical composition, chemical composition, and plant height, and principal component analysis (PCA) was used to describe multivariate trait patterns. The year strongly affected mixture performance. Mean dry matter yield decreased from 6949 ± 212 kg ha⁻¹ in 2023 to 1588 ± 94 kg ha⁻¹ in 2024, corresponding to an approximately 76% reduction. In 2023, forage mixtures produced the highest yield, followed by universal and grazing mixtures, whereas in 2024, differences among mixture types were not significant. Botanical composition shifted toward a higher legume proportion in 2024, while mixture type differences in chemical composition largely converged, except for DMD. PCA showed clearer separation among mixture types in 2023, with PC1 and PC2 explaining 64.9% and 17.6% of variance, respectively, whereas separation weakened in 2024. These results indicate that mixture composition and intended use influenced productivity and quality mainly under more favorable growing conditions, while year-to-year variation strongly constrained first-cut mixture performance. Full article

This study evaluated the effects of replacing 25% of wheat straw with dried carob (Ceratonia siliqua) leaves and twigs, either untreated or treated with 5% sodium hydroxide (NaOH), on growth performance, nutrient digestibility, carcass traits, meat quality, blood metabolites, and rumen microbial populations in Assaf lambs. Twenty-four male lambs (2.5 months old; 29 ± 0.5 kg) were randomly assigned to three dietary treatments (n = 8): a control diet containing wheat straw as the sole roughage source, supplemented with a concentrate feed, a diet with 25% untreated carob leaves and twigs (UCL), and a diet with 25% NaOH-treated carob leaves and twigs (TCL). Following a 14-day adaptation period, lambs were fed the corresponding experimental diet for 14 weeks. Carob inclusion improved growth performance, with UCL lambs showing the highest average daily gain (214 g/d) compared with TCL (201 g/d) and control (160 g/d), resulting in improved feed conversion ratio (9.02 vs. 5.68 and 5.63, respectively) (p < 0.001). Blood urea nitrogen was reduced (p < 0.001) in UCL lambs (26.8 vs. 38.5 mg/dL in control), suggesting improved nitrogen retention. Digestibility responses differed between treatments (p < 0.001), as TCL increased dry matter digestibility to 72.6% compared with 65.4% (UCL) and 63.6% (control), indicating enhanced nutrient utilization following NaOH treatment. Both UCL and TCL increased (p < 0.001) carcass weights (up to 24.7 vs. 21.0 kg in control), while TCL achieved the highest dressing percentage (46.6% vs. 43.4%). Meat quality traits were generally unaffected in terms of color (lightness, redness, and yellowness) and water-holding capacity; however, shear force decreased from 33.6 N (control) to 30.0 N (TCL), indicating improved tenderness. Carob inclusion modified meat composition by increasing (p < 0.001) lipid content (12.0–12.2 vs. 9.6%) and improving fatty acid profile, with reduced saturated fatty acids (53.4–56.5 vs. 61.4%) and increased α-linolenic acid (2.04 vs. 1.58%), leading to a lower n-6/n-3 ratio (5.54–5.61 vs. 6.45). Rumen fermentation was also affected (p < 0.001), as carob diets increased total bacterial populations and reduced protozoal counts, suggesting shifts toward more efficient microbial activity. In conclusion, replacing 25% of wheat straw with carob leaves improved growth performance and feed efficiency, with untreated carob primarily enhancing nitrogen utilization and treated carob improving fiber digestibility and carcass yield. These findings support the use of carob by-products as a viable alternative feed resource, although responses depend on processing method and targeted production outcomes. Full article

Optimizing harvest time and oil production requires accurate olive fruit quality characterization. Traditional chemical methods are costly and tedious, leading to poor monitoring resolution and reliance on subjective visual assessments. While spectroscopy offers a non-destructive alternative, standard equipment remains complex and prohibitively expensive for smallholder farmers. To address this, we propose a methodology using a custom-made, low-cost multispectral device. Built upon the AS7265x board, the system acquires 18 spectral bands in the visible and near-infrared range (410–940 nm). We used these spectral data to feed artificial neural network (ANN) models for estimating the quality of intact olives. During a two-season field experiment, we monitored ripening to acquire spectral signatures and ground-truth values for oil content per fresh weight (OCFW), oil content per dry matter (OCDM), moisture (M), and titratable acidity (TA). External validation showed high accuracy for OCFW (R²p = 0.86), OCDM (R²p = 0.86), and M (R²p = 0.89), proving the system’s reliability. However, TA estimation showed lower performance (R²p = 0.21), indicating limited spectral correlation. These findings pave the way for affordable, real-time smart farming tools for olive quality monitoring. Full article

Reducing nitrogen (N) fertilization is essential for sustainable agriculture, but it frequently suppresses photosynthetic capacity and diminishes grain yield in sorghum. To determine whether exogenous brassinolide (BL) can offset these negative effects, a two-year field experiment was conducted using foliar BL application (0.1 mg L⁻¹) under three N levels (0, 75, and 150 kg N ha⁻¹), with assessments of grain yield, photosynthetic parameters, dry matter accumulation, and nitrogen use efficiency (NUE). Results showed that BL significantly increased grain yield under zero N (by 15.47%) and moderately under 50% N reduction (by 4.32%), primarily by increasing grains per panicle. Under N-reduced conditions, BL enhanced net photosynthetic rate (Pn), chlorophyll content, Rubisco/PEPC activities, and dry matter partitioning to panicles, with these traits positively correlated with yield. Under 50% N reduction, BL improved N recovery efficiency (RE) and agronomic efficiency (AE) while leaf N content correlated positively with SPAD, Pn, and yield. No significant BL effects occurred under normal N. Thus, exogenous BL application partially compensates for N reduction-induced yield loss by enhancing photosynthesis, source–sink partitioning, and NUE, providing a promising, environmentally sustainable strategy for sorghum production under reduced N input. Full article

To clarify the effects of biochar application on leaf photosynthesis, dry matter accumulation, and productivity in a maize–soybean intercropping system, a two-year field experiment was conducted in the Yellow River irrigation area of Inner Mongolia from 2024 to 2025. A split-plot design was adopted with two biochar application rates (0 and 5 t ha⁻¹) and three cropping patterns, including maize monoculture, soybean monoculture, and maize–soybean 2:4 intercropping. Leaf SPAD values, photosynthetic characteristics (Pn, Tr, Gs, and Ci), yield components, and land equivalent ratio (LER) were determined. Compared with maize monoculture, intercropping significantly increased maize SPAD values at the V12 and VT stages by 12.80% and 13.39% in 2024 and by 15.41% and 20.58% in 2025, respectively, and enhanced maize Pn, Tr, and Gs at the V12 and R1 stages. Soybean showed greater sensitivity to intercropping, with reduced SPAD values, Pn, Tr, and Gs during the branching, flowering, and pod-setting stages, whereas biochar application partially alleviated these inhibitory effects. Intercropping increased maize kernel number per ear and thousand-kernel weight but reduced soybean effective plant density, grain number per plant, and grain yield. Biochar application improved the grain yield of both intercropped maize and soybean. Under biochar application, the LER values reached 1.04 in 2024 and 1.21 in 2025, indicating a clear advantage in land-use efficiency. Overall, biochar application and maize–soybean intercropping were associated with improved photosynthetic performance, higher land-use efficiency, and increased system productivity. Full article

Enhancing both grain yield and water productivity (WP) under water scarcity is a critical challenge for sustainable maize production. Conventional farmer practices—characterized by low planting density (D₁: 9.0 × 10⁴ plants·ha⁻¹) and excessive irrigation (I₅)—often limit the potential for both. To advance the high-density production system enabled by precision stage-specific regulation (HD-PSR), we conducted a multi-region field experiment across arid to sub-humid climates in Xinjiang, China. Treatments included region-specific full irrigation (I₅) and four deficit levels (I₁–I₄, each reduced by 90 mm relative to I₅). Compared with the conventional model (D₁ + I₅), the HD-PSR-based optimized model (D₂, 12.0 × 10⁴ plants·ha⁻¹ plus regulated deficit irrigation, I_opt) synergistically increased grain yield by 18.8–25.0% and WP by 8.7–20.0% across sites, while saving 11.1–50.0% of irrigation water. This synergy was driven by improved canopy coverage and sustained dry matter accumulation under precision-regulated irrigation that maintained soil water storage at ~70% of field capacity, thereby reducing non-productive water losses (soil evaporation in arid/semi-arid areas and excessive transpiration in the semi-humid area). Our study provides a quantitative irrigation module for the HD-PSR and identifies a translatable soil water threshold (~70% FC) for sustainable intensification of maize in water-limited regions. Full article

This research assessed the impact of probiotic culture incorporation and the sous vide manufacturing method on the physicochemical, textural, colorimetric, and microbiological characteristics of yoghurts during the storage duration. The trials used conventional and sous vide manufacturing techniques using probiotic and classical starting cultures, and the products were analyzed throughout the storage period. The findings indicate that the use of probiotic cultures significantly enhances organic acid synthesis. This rise resulted in a lower pH (≈4.54) and increased titratable acidity (≈1.60%). Furthermore, it has been shown that the total organic acid concentration, mostly lactic acid, rose (≈24,045 mg/kg), while concurrently, the yeast-mold load decreased (≈2.69 log CFU/g). Throughout the storage duration, a reduction in pH and an elevation in acidity and microbial activity were seen in all samples (p < 0.05). The sous vide manufacturing method, due to regulated heat processing and a sealed system design, has decreased syneresis (≈5.65%) and an enhanced dry matter content (≈13.09%). This circumstance has resulted in the development of a more uniform gel structure, thereby enhancing the textural attributes. Color investigations indicated that the integration of probiotics and sous vide decreased the ΔE values, and in samples with ΔE < 3, the color change was imperceptible to the human eye. In conclusion, the simultaneous application of probiotic culture addition and the sous vide production technique has exhibited a synergistic effect on acidity development, microbial stability, textural properties, and color stability, indicating its efficacy in producing higher-quality functional yoghurt. Full article