Search Results (215)

The gut microbiota plays a fundamental role in human physiology by influencing metabolism, immunity, and neuroendocrine communication. Growing evidence suggests that physical exercise modulates gut microbial composition; however, study findings remain inconsistent due to variations in design, training type, and population characteristics. This review summarizes current research on how different forms, intensities, and frequencies of exercise shape the gut microbiota and discusses their implications for athlete health and performance. Moderate and sustained physical activity generally promotes higher microbial diversity, increases short-chain fatty acid (SCFA)-producing bacteria, and enhances gut barrier integrity. Endurance training, particularly long-term, is most consistently associated with beneficial microbial shifts, including increases in Prevotella, Akkermansia, and Faecalibacterium. In contrast, excessive or high-intensity endurance exercise was shown to cause dysbiosis, inflammation, and greater intestinal permeability. Resistance training appears to induce milder changes but was shown to improve mucin synthesis and butyrate production, especially in older adults. Exercise frequency also plays a role, with regular daily training enriching metabolic pathways linked to gut and systemic health. Overall, the impact of exercise on the gut microbiota depends on the type, intensity, and duration of activity. Balanced, moderate exercise combined with a healthy diet emerges as the most effective strategy to enhance microbial diversity, reduce inflammation, and support overall performance and well-being in athletes. Full article

Irritable bowel syndrome (IBS) is a disorder of gut–brain interaction characterized by recurrent abdominal pain associated with a change in the frequency and/or form of stools. Approximately one in three patients with quiescent inflammatory bowel disease (IBD), defined as the absence of endoscopic evidence of active inflammation, experience IBS-type symptoms. These symptoms are associated with reduced quality of life and increased psychological burden, and can complicate clinical assessment by mimicking conditions such as small intestinal bacterial overgrowth, bile acid malabsorption, or post-inflammatory complications. This up-to-date narrative review examines the mechanisms, diagnostic challenges, and management of IBS-type symptoms in quiescent IBD. Evidence suggests that these symptoms arise from a complex “matrimony” of functional and organic processes, including low-grade residual inflammation, altered intestinal permeability, microbiota dysbiosis, visceral hypersensitivity, and psychosocial impairment. Diagnosing IBS-type symptoms in IBD requires a “positive”, symptom-focused approach while carefully excluding active inflammation. Management should adopt a biopsychosocial approach, integrating dietary strategies (e.g., low-FODMAP diet), brain–gut behavioral therapy, biofeedback therapy, and/or pharmacological treatments such as antispasmodics, antidiarrheals, laxatives, and neuromodulators to address both physiological and psychological factors. Future research should integrate sensitive biomarkers and longitudinal follow-up to enhance diagnostic precision and guide personalized therapy. Understanding and addressing the overlap between IBS and IBD is essential to reduce the multidimensional burden on physical health, psychological well-being, and daily functioning. Full article

Background/Objectives: Diet and physical activity are key lifestyle behaviours associated with sleep quality, yet their combined and sex-specific associations remain insufficiently understood. This study examined the associations between dietary behaviours and sleep quality among university students and assessed whether physical activity formed part of an indirect statistical association between these variables, with sex considered as a moderator. Methods: A cross-sectional study was conducted among 418 students (199 males, 219 females) from the Wroclaw University of Health and Sport Sciences. Body height and body mass were measured using standard anthropometric procedures. Sleep quality (SQ) was registered with the Pittsburgh Sleep Quality Index (PSQI), dietary habits were assessed with the Questionnaire of Eating Behaviours (QEB) and physical activity (PA) was assessed with the International Physical Activity Questionnaire (IPAQ). Data-driven feature-selection methods were applied to identify dietary behaviours associated with sleep quality, which were combined into a Synthetic Dietary Behaviour Index (SDBI). A moderated mediation model, adjusted for body mass index (BMI), was then used to examine the statistical associations between dietary behaviours, physical activity, sleep quality, and sex. Sleep quality was modelled as a continuous PSQI score in mediation analyses, while the dichotomised PSQI category was used only for feature selection. Results: Machine-learning feature selection identified nine dietary behaviours statistically associated with sleep quality. Unfavourable behaviours—fast food, fried meals, sweetened beverages, energy drinks and alcohol—were linked to poorer sleep, whereas vegetables, curd cheese and wholegrain bread were associated with better sleep. Poor sleep was more prevalent among females (45.2% vs. 14.6%, χ² (1) = 65.4, p < 0.001). The mediation model indicated that physical activity formed part of a statistically significant but modest indirect association between dietary behaviour and sleep quality, with sex moderating the IPAQ → PSQI path (β = −0.45, p = 0.006). Indirect associations were significant for both sexes but stronger among females (males: β = 0.032, p = 0.021; females: β = 0.102, p = 0.004). Conclusions: Unfavourable dietary patterns and lower physical activity were statistically associated with poorer sleep quality, with a stronger indirect statistical effect observed among females. These findings support the relevance of integrated, sex-sensitive lifestyle approaches addressing both dietary behaviours and physical activity, while acknowledging the cross-sectional nature of the data. Full article

This study examines the critical role of whole milk or milk replacer as a liquid diet (LD) with 15% solids in combination with different physical forms of starter as a solid diet (SD), on performance, health, and behavior of pre-weaned calves. Sixty male Holstein calves were used in a 2 × 2 factorial design, and randomly distributed into the following treatments: Whole milk powder diluted to 12.5% of solids and enriched with 25 g/L of milk replacer to achieve 15% solids, associated with either micropelleted stater (WM+micro) or texturized stater (WM+text); milk replacer diluted to 15% solids associated with either micropelleted stater (MRmicro) or texturized stater (MRtext). Starter intake and, consequently, total DMI were higher in the MRtext treatment compared to WM+micro. Calves fed texturized starter showed higher DMI, starter intake time, and rumination time. Calves in the WM+Text group showed greater ADG compared with MR treatments, regardless of starter type. Calves fed WM+ presented a lower number of days with fecal score ≥2, and the first day of diarrhea occurred at older ages. Calves fed MR showed more health challenges but similar feed efficiency with WM+, while texturized starter increased intake, eating duration, and rumination compared with micropelleted starter. Full article

Physical inactivity, which currently dominates the lifestyles of most people, is linked to chronic metabolic diseases such as obesity, type 2 diabetes (T2D), hypertension, metabolic syndrome, and cardiovascular disease, all of which share insulin resistance as a common pathogenic mechanism. Both epidemiological and experimental intervention studies have consistently shown that physical activity and exercise can reduce the incidence of these diseases and significantly improve their clinical outcomes, resulting in enhanced quality of life and well-being. This approach includes various forms of aerobic and anaerobic/resistance training, either individually or in combination, leading to reduced insulin resistance and visceral fat, regardless of the weight loss achieved through diet. It also lowers inflammatory responses and oxidative stress, a harmful mechanism that leads to cellular damage, and positively impacts immunological regulation. Regarding timing, physical activity/exercise appears to produce better outcomes for metabolic control, particularly in individuals with T2D, when performed after dinner compared to other times of the day. In addition to organized physical activity/exercise sessions, practices such as interrupting prolonged sitting with frequent breaks every 30 min that involve muscular contractions and increased energy expenditure may also benefit metabolic health. Minimizing physical inactivity, prolonged sitting at work or during leisure time, can decrease the frequency of metabolic illness, enhance health and quality of life, and avert premature death. However, intense exercise may not always be the most beneficial option for health, and the relationship between adverse events and the intensity of physical activity or exercise resembles a U-shaped or J-shaped curve. Physical activity/exercise should be performed at a suitable intensity that aligns with personal capability. In this primarily clinically focused review, we discuss the effects of insulin on target tissues, the significance of insulin sensitivity in metabolic regulation, how physical inactivity contributes to insulin resistance, the different types of exercise and their impact on insulin effectiveness, and the importance of physical activity and exercise in managing metabolic diseases. Full article

Cultivated carrot (Daucus carota subsp. sativus) is a valuable component of equine diets due to its high β-carotene content (a precursor of vitamin A) and desirable sensory properties. However, its use may be limited by short shelf life, susceptibility to spoilage, and firm texture, which can be challenging for horses with dental problems. This study aimed to evaluate horses’ preferences for different physical forms of carrots. Twenty-one leisure horses aged 3–22 years were individually evaluated in a free-choice test. Each horse was simultaneously offered equal portions (150 g) of five carrot forms: raw, grated, boiled, dried, and juice. Feeding time and selection order were recorded, and data were analyzed using non-parametric tests. The form of carrots had a significant effect on feeding time (p < 0.001). Mean feeding time differed among forms; boiled carrots were consumed in 78.4 ± 15.3 s, whereas dried carrots required 156.2 ± 28.7 s. Among solid forms, raw carrots were most frequently selected first, followed by grated and dried carrots, while boiled carrots were the least preferred (p < 0.01). Carrot juice was initially rejected by most horses, but its acceptability increased in later trials. No significant correlations were found between horse age and either feeding time or selection order. The findings emphasize the practical importance of carrot processing in improving feed management and meeting horses’ individual nutritional needs. Full article

Background/Objectives: Type 2 diabetes mellitus (T2DM) is a prevalent chronic disease frequently associated with impaired nutritional status and reduced health-related quality of life (HRQoL), especially in older adults. Alongside pharmacological treatment and diet, physical exercise has emerged as a complementary strategy. Pilates, a mind–body discipline focused on controlled movement and postural alignment, may help improve outcomes beyond conventional care. This study aimed to evaluate the effectiveness of a 12-week Pilates intervention on nutritional status and HRQoL in older adults with T2DM. Methods: A randomized controlled trial was conducted with 104 older adults diagnosed with T2DM. The participants were randomly allocated to a Pilates group (n = 52) or a control group (n = 52). The intervention consisted of 24 supervised sessions delivered twice weekly for 60 min over 12 weeks. Nutritional status was assessed using the Mini Nutritional Assessment (MNA), and HRQoL was measured with the 12-Item Short Form Health Survey (SF-12). Both assessments were carried out at baseline and after the intervention. Results: Post-intervention scores indicated better nutritional condition and higher ratings in both physical and mental dimensions of HRQoL, while no significant changes were observed in controls. Conclusions: A structured Pilates program improved nutritional status and HRQoL in older adults with T2DM. These results suggest that Pilates is a feasible, safe, and effective complementary therapy in the comprehensive management of this population. Full article

Chronic pain conditions are debilitating and have an enormous impact on quality of life, yet underlying biological mechanisms remain poorly understood, hindering the development of diagnostic tools and effective treatments. Emerging evidence suggests a role for immune dysfunction in chronic pain. Among the various forms of immune dysfunction, T cell exhaustion and senescence, well-characterized in cancer and chronic infections, remain largely unexplored in chronic pain research. At the same time, lifestyle factors such as sleep, stress, physical activity, and diet are increasingly recognized as modulators of both pain and immune function. This review explores the potential interplay between these behavioural factors, immune exhaustion/senescence, and chronic pain. Critical gaps in current knowledge are identified, and future directions are outlined to clarify immune dysfunction and the influence of lifestyle factors in chronic pain conditions. Full article

Background: Obesity and insomnia are prevalent public health issues with shared behavioral and physiological pathways. However, their interplay remains understudied in occupational cohorts. Obesity and insomnia are prevalent public health issues with shared behavioral and physiological pathways. However, their interplay remains understudied in occupational cohorts. This study aimed to evaluate the associations of sociodemographic factors, lifestyle habits, and insomnia severity with multiple obesity indices in a large population of Spanish workers. Methods: We conducted a cross-sectional analysis of 84,898 workers (2021–2024). Data were obtained from annual occupational health assessments conducted across multiple Spanish regions between 2020 and 2024. Insomnia severity was assessed using the Insomnia Severity Index (ISI), dietary quality using the 14-item Mediterranean Diet Adherence Screener (MEDAS-14), and physical activity using the International Physical Activity Questionnaire—Short Form (IPAQ-SF). Adiposity was measured using Body Mass Index (BMI), waist-to-height ratio (WtHR), the Clínica Universidad de Navarra–Body Adiposity Estimator (CUN-BAE), and the Metabolic Score for Visceral Fat (METS-VF). Multivariable logistic regression models were used to examine associations adjusted for age, sex, education, and occupational social class. Results: Higher ISI scores were significantly and independently associated with elevated adiposity across all indices, with the strongest association observed for METS-VF (odds ratio = 1.19, 95% CI 1.14–1.25, p < 0.001). Women showed higher mean CUN-BAE and METS-VF values than men (CUN-BAE: 37.4 ± 6.2 vs. 25.6 ± 6.4; p < 0.001; METS-VF: 5.7 ± 0.7 vs. 6.4 ± 0.6; p < 0.001), despite lower BMI (25.3 ± 4.8 vs. 26.8 ± 4.3; p < 0.001). Lower physical activity (OR = 5.70; 95% CI 4.91–6.50), poor adherence to the Mediterranean diet (OR = 3.29; 95% CI 2.88–3.70), smoking (OR = 1.29; 95% CI 1.22–1.36), and lower occupational class (Class III: OR = 1.77; 95% CI 1.56–1.97) were also significantly associated with higher obesity markers. Associations were more pronounced among women and participants with severe insomnia symptoms. Conclusions: Insomnia severity, sociodemographic disadvantage, and unhealthy behaviors (low physical activity, poor diet, smoking) were all independent correlates of general and visceral adiposity. The findings underscore the need for comprehensive workplace health programs that integrate sleep quality assessment, dietary improvement, and physical activity promotion to prevent obesity and its metabolic consequences. Full article

Overweight, including its severe form obesity, among children and adolescents has risen rapidly in Latin America. Schools play a critical role in addressing this growing public health challenge, as they offer a structured setting to implement preventive interventions targeting nutrition literacy, physical activity, and the food environment. The aim of this article is to describe the effectiveness of school-based interventions for preventing overweight in Latin America and whether existing policies, programs and other initiatives in the region align with the best available evidence. Among the 27 interventions included, most were conducted in Chile (41%), used a pre–post design (41%), adopted a preventive approach (85%), and reported positive effects (52%). Effective interventions included activities on nutrition literacy, physical activity, nutritious foods and diets, provision of free and safe drinking water (e.g., water that is free from microbial contamination and suitable for drinking), and healthy food environment. Experimental studies showed that the duration of effective interventions ranged from two months to two years and were primarily directed at primary school students including parents and teachers. Argentina, Chile, Colombia, Mexico, Peru, and Uruguay had multi-component policies and programs under a regulatory framework (e.g., laws or regulations passed by a government) based on the best available evidence to prevent overweight in school-aged children and adolescents. Only a limited number of countries have implemented these interventions. Ensuring program sustainability is critical to inform evidence-based childhood overweight prevention policies in the region. Policymakers should use the best scientific evidence to guide childhood overweight prevention strategies. Full article

Background: Albania is undergoing rapid nutrition transition, yet cardiometabolic (CM) risk in young adults is poorly characterized. We report baseline, cross-sectional findings from a WHO-aligned sentinel study examining diet, physical activity and early CM phenotypes, with fat quality examined as a modifiable exposure. Methods: Young adults recruited on campus (n = 262; median age, 21 years; 172 women, 90 men) underwent standardized anthropometry, seated blood pressure (BP) and fasting glucose (FG). Diet was assessed by two interviewer-administered 24 h recalls and activity outlined by the IPAQ-short form. We derived potential renal acid load (PRAL) and a MASLD-oriented nutrient score, computed a composite CM risk score (cCMRS: sex-standardized mean of WHtR, mean arterial pressure, FG) and fitted prespecified energy-partition models for isocaloric +5% of energy substitutions (SFA → PUFA; SFA → MUFA) with Benjamini–Hochberg false discovery rate (FDR) control. Results: Despite normal average BMI (23.4), risk clustering was common: elevated BP in 63% of men and 30% of women, impaired FG (100–125 mg/dL) in almost one third and central adiposity (WHtR ≥ 0.5) in 51% of men and 24% of women. Diets were SFA-rich (~17–19%E), sodium-dense and low in fiber and several micronutrients (e.g., vitamin D, folate, potassium). In isocaloric models, SFA → PUFA was associated with more favorable nutrient signatures: MASLD-oriented score −28% (p < 0.001; FDR-significant) and PRAL −33% (p = 0.007; FDR-borderline/suggestive). Conclusions: A waist-centric CM subphenotype—central adiposity co-occurring with upward BP shifts and intermittent dysglycemia—was detectable in young adults despite normal average BMI, against a background of poor diet quality and low activity. These baseline surveillance signals are not causal effects. Integration into routine with WHO-aligned NCD surveillance is feasible. Prospective follow-up (biomarker calibration, device-based activity, repeated waves) will refine inferences and inform scalable proactive prevention. Full article

Background: Atherosclerosis is a leading cause of cardiovascular morbidity and mortality worldwide. Although lipid-derived atherogenic indices are widely used for cardiovascular risk assessment, their relationship with sociodemographic factors, lifestyle behaviors, and health-related quality of life (HRQoL) in occupational populations remains insufficiently explored. This study aimed to evaluate the association between atherogenic risk, measured by total cholesterol/high-density lipoprotein cholesterol (TC/HDL-c), low-density lipoprotein cholesterol/high-density lipoprotein cholesterol (LDL-c/HDL-c), triglyceride/high-density lipoprotein cholesterol (TG/HDL-c), and atherogenic dyslipidemia (AD) and sociodemographic, lifestyle, and HRQoL variables in a large cohort of Spanish workers. Methods: We conducted a cross-sectional analysis of 100,014 Spanish workers aged 18–69 years, of whom 39.9% were women, with a mean age of 38.2 years (SD 10.2 or IQR) and 38.9 years (SD 10.3 or IQR) for men, during the health examinations carried out between 2021 and 2024. Sociodemographic variables included sex, age group, and occupational social class. Lifestyle factors comprised smoking status, adherence to the Mediterranean diet (MEDAS score), and physical activity (IPAQ categories). HRQoL was assessed using the 12-item Short Form Survey (SF-12), stratified into good vs. poor categories. Logistic regression models were applied to estimate odds ratios (OR) and 95% confidence intervals (CI) for moderate-to-high atherogenic risk across indices, adjusting for potential confounders. Results: Men exhibited a lower likelihood of moderate-to-high TC/HDL-c and LDL-c/HDL-c but a markedly higher probability of elevated TG/HDL-c and AD compared to women (OR range: 0.42–3.67, p < 0.001). A clear age-related gradient was observed across all indices, with participants aged 60–69 showing the highest risk (OR range: 2.28–7.84, p < 0.001). Lower social class, smoking, physical inactivity, poor diet, and poor SF-12 scores were significantly associated with increased atherogenic risk, with physical inactivity (OR up to 8.61) and poor diet (OR up to 4.98) emerging as the strongest predictors. Conclusions: Atherogenic risk in this large working cohort is strongly influenced by both traditional cardiovascular risk factors and HRQoL. Integrating lifestyle modification and quality-of-life improvement strategies into workplace health programs could substantially reduce the atherogenic burden. Longitudinal research is needed to confirm these associations and guide targeted interventions. Full article

Background: Lifestyle behaviours, such as dietary patterns, sleep quality, perceived stress, and physical activity, are closely interconnected and play a critical role in maintaining health and well-being. Among school teachers, a profession marked by high psychosocial demands, the interplay between these factors warrants further exploration. Objective: This pilot study aimed to explore the associations between adherence to the Mediterranean Diet (MD), sleep quality, perceived stress, and physical activity levels among Portuguese primary and secondary school teachers. Methods: A cross-sectional study was conducted between April and December 2023. Participants completed validated self-reported instruments, including the MD Adherence Screener (MEDAS), the Pittsburgh Sleep Quality Index (PSQI), the Perceived Stress Scale (PSS-10), and the short form of the International Physical Activity Questionnaire (IPAQ-SF). Correlational analyses and multivariable linear regression models were applied to explore the relationships among the variables. Results: Among the 50 teachers, 32% demonstrated high adherence to the MD, 60% reported good sleep quality, 62% experienced moderate stress, and 44% engaged in high physical activity. Women were more likely to adhere strongly to the MD (p = 0.012). Higher MD adherence was positively associated with physical activity (ρ = 0.343; p = 0.015). A positive correlation was observed between perceived stress and poorer sleep quality (ρ = 0.346, p = 0.014), and a negative correlation between perceived stress and physical activity levels (ρ = −0.297, p = 0.036). Despite reporting good sleep quality, these participants had higher perceived stress scores (p = 0.015). In adjusted models, sleep quality was the only significant predictor of perceived stress (B = 0.708; p = 0.003), and vice versa (B = 0.267; p = 0.003), suggesting a bidirectional relationship. Conclusions: The findings highlight the interrelation between dietary pattern, sleep stress, and physical activity in a professional group vulnerable to lifestyle-related health challenges. Although the sample size limits generalisability, this study provides preliminary evidence supporting the need for integrated health promotion strategies targeting stress reduction and lifestyle optimisation among educators. Full article

Background: Celiac disease (CD) is a chronic, immune-mediated condition requiring lifelong adherence to a gluten-free diet. In children, CD can negatively impact not only physical health but also psychological well-being and quality of life. The burden of dietary restrictions, social limitations, and emotional stress may lead to increased anxiety and depressive symptoms. This study aims to compare the quality of life, anxiety, and depression levels in children with celiac disease to those of healthy peers without chronic illness. Methods: The research involved a total of 129 individuals aged 8–18 years (64 with celiac disease and 65 healthy volunteers) and their parents. To assess children with celiac disease and healthy children, we used a sociodemographic form that we created, along with the State-Trait Anxiety Inventory (STAI), Trait Anxiety Inventory (TAI), Children’s Depression Inventory (CDI), Pediatric Quality of Life Inventory (PedsQL), and Parent Quality of Life Inventory tests. Results: Celiac patients’ diet adherence, parental education level, and family income were found to be significantly associated with quality of life, as well as levels of depression and anxiety. (p < 0.037, p < 0.04, p < 0.004, respectively). Celiac patients had significantly lower BMI SDS (mean −0.55 ± 1.13, p < 0.001) and height SDS scores (mean −0.49 ± 1.28, p < 0.017). Key factors negatively affecting the quality of life in individuals with celiac disease were difficulty adhering to the diet and low family income levels. Conclusions: Elevated anxiety with reduced quality of life highlights the importance of integrating psychosocial support into the routine care of children with celiac disease. A holistic treatment approach that considers the psychosocial well-being of children can significantly improve their quality of life. Full article

The diet of dairy calves can be contaminated with mycotoxins, posing a potential risk to animal health. This case study report aimed to make the first assessment of the presence of multiple mycotoxins in concentrates fed to dairy calves in Brazil. A total of 19 concentrate samples intended for dairy calves were analyzed using liquid chromatography coupled with mass spectrometry. Aflatoxins, deoxynivalenol, and T-2 toxin were not detected in any samples, whereas fumonisins B₁ (FB₁) and B₂ (FB₂) were present in 100% of the samples, with mean concentrations of 2750.1 μg/kg and 834.9 μg/kg, respectively. Zearalenone (ZEN) was detected in 36.8% of samples, with a mean concentration of 929.9 μg/kg. Significant correlations were observed between FB₁ and FB₂ (ρ = 0.978; p < 0.001) and between FB₂ and ZEN (ρ = 0.735; p = 0.05). While the physical form of the concentrate did not influence (p > 0.05) mycotoxin concentrations, a trend was observed for FB₁ (ρ = −0.417; p = 0.07) and FB2 (ρ = −0.395; p = 0.09). These findings highlight the frequent occurrence of Fusarium mycotoxins, likely due to pre-harvest contamination, emphasizing the potential risk of additive or synergistic effects in dairy calves. Full article