Search Results (833)

The black soldier fly (BSF), Hermetia illucens, has gained increasing attention as a sustainable protein source for animal feed. This study investigated the effects of dietary supplementation with hemp seed oil (HSO) at 0.5–6% concentrations on the growth performance and nutritional composition of black soldier fly larvae (BSFL). Larval development, survival rate, body weight, and adult longevity were evaluated under controlled conditions. In addition, chemical characterization of HSO was performed, and the proximate composition, mineral content, and amino acid profile of dried larvae were analyzed. The results indicated that HSO supplementation had no statistically significant effect on developmental time, survival rate, biomass accumulation, or adult lifespan compared to the control. Gas Chromatography–Mass Spectrometry (GC-MS) profiling of HSO revealed a wide range of bioactive compounds, including unsaturated fatty acids (UFAs), phytosterols, cannabinoids, and tocopherols. The BSFL showed high levels of fat and energy, with essential amino acids and minerals present in favorable concentrations for feed applications. The HSO improves the protein levels at the 0.5–1.0% concentration, with negative correlations at higher concentrations. The findings suggest that HSO can be integrated into BSFL diets without adverse effects on growth performance, while potentially enhancing the functional value of the larvae. This supports the feasibility of incorporating hemp by-products into insect-rearing systems to promote circular and value-added feed production. Full article

Metallic glass, as an emerging catalytic material, possesses an atomic structure characterized by long-range disorder and short-range order, which creates abundant and accessible active sites that enhance the adsorption and reactivity toward pollutant molecules, particularly dye compounds. In treating highly colored and recalcitrant Reactive Black 5 (RB5) dye wastewater, Mo₅₁Fe₃₄B₁₅ metallic glass wire demonstrate outstanding catalytic degradation performance within a conventional Fenton-like system. Under acidic conditions (pH = 2), the material exhibits a degradation rate constant of 0.698 min⁻¹ for a 20 ppm RB5 dye solution, achieving a degradation efficiency of 98.8% within 10 min. After 10 consecutive cycles, the efficiency remains at 95%, and throughout 15 cycles, it consistently maintains a performance level above 90%. As the reaction proceeds, the degradation rate gradually decreases, primarily due to the accumulation of corrosion products on the catalyst surface, which are predominantly composed of MoO₃ and Fe₂O₃. During the degradation process, metallic Mo⁰ and Fe⁰ serve as electron donors that facilitate the decomposition of H₂O₂, generating highly reactive hydroxyl radicals (•OH). These radicals attack the chromophoric structure of the dye, leading to its structural disruption and enabling rapid decolorization. Full article

With the rapid expansion of industrial activities, the accumulation of heavy metals in the environment has become a serious threat to ecological security and public health. Rhizosphere microorganisms play a crucial role in supporting the growth and quality of medicinal plants by facilitating nutrient uptake and regulating hormonal balance. However, medicinal plants can absorb heavy metals from contaminated soils during growth, resulting in toxic metal accumulation in plant tissues and reduced efficacy of active compounds. At the same time, excessive heavy metal levels suppress rhizosphere microbial growth and activity, disrupt community structure and function, and weaken their beneficial interactions with plants. These processes collectively lead to soil fertility decline, hindered plant development, and compromised safety and quality of medicinal materials. This review systematically summarizes the mechanisms by which heavy metals affect medicinal plants and their rhizosphere microbiota, and highlights that future research should focus on elucidating these interactions, developing advanced remediation technologies, and establishing comprehensive monitoring systems for the quality and safety of medicinal plants, thereby providing a scientific basis for their safe utilization and quality improvement. Full article

Antibiotic use in aquaculture has become widespread to sustain production and control bacterial diseases, but it poses significant ecological and human health risks due to residue accumulation and resistance development. This study investigated the occurrence, dominance, and combined use of sulfonamide and fluoroquinolone antibiotics in freshwater fish aquaculture ponds around Wuxi, China. Here, the term aquaculture refers specifically to the controlled farming of freshwater fish species such as carp and crucian carp in managed pond systems. A total of 80 water samples (collected exclusively from pond waters) were obtained from 40 ponds during the high intensity rearing and harvest stage of fish. Residues of enrofloxacin and sulfonamide antibiotics were analyzed using a validated LC–MS/MS method with detection limits in the low nanogram-per-liter range. Results revealed that antibiotics were ubiquitous in pond waters, with enrofloxacin emerging as the dominant compound in August, reaching concentrations of up to 2.36 µg/L. By October, sulfonamides, particularly sulfamethoxazole and sulfadiazine, became more prevalent, with a maximum sulfadiazine concentration exceeding 4 µg/L. Multivariate analyses demonstrated a clear seasonal shift in antibiotic profiles, while correlation analyses indicated limited combined use in summer but notable co-occurrence of sulfonamides in autumn. These findings underscore that antibiotic application patterns in aquaculture are strongly linked to production stages, with potential consequences for environmental safety, resistance development, and food security. Effective monitoring, stricter regulation, and alternative disease management strategies are urgently required to mitigate risks and promote sustainable aquaculture practices. Full article

Age-related macular degeneration (AMD) is a leading cause of irreversible visual impairment in the elderly, and oxidative stress, primarily mediated by reactive oxygen species (ROS), is widely recognized as a central driver of its onset and progression. The retina is highly susceptible to oxidative damage due to its elevated oxygen consumption, abundant polyunsaturated fatty acids, and continuous exposure to light. Recent studies have elucidated molecular mechanisms in which mitochondrial dysfunction, disruption of redox homeostasis, inflammation, and complement activation interact to promote degeneration of retinal pigment epithelium (RPE) and photoreceptor cells. In addition to age-related oxidative stress, environmental factors such as motor vehicle exhaust and volatile organic compounds (VOCs) can accelerate the accumulation of lipofuscin and drusen, thereby fostering a chronic pro-inflammatory milieu. From a therapeutic perspective, beyond conventional antioxidant supplementation, emerging strategies targeting oxidative stress-related pathways have gained attention, including mitochondrial protectants, activation of the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway, anti-inflammatory agents, and gene therapy. Importantly, several innovative approaches are under investigation, such as saffron supplementation with neuroprotective properties, drug repositioning of levodopa, and nanotechnology-based delivery systems to enhance retinal bioavailability of antioxidants and gene therapies. This review summarizes the pathophysiological role of oxidative stress in AMD from a molecular mechanistic perspective and discusses recent advances in research and novel therapeutic targets. Full article

TAAR1 agonists have emerged as promising therapeutic agents capable of modulating glucose homeostasis, enhancing insulin secretion and suppressing appetite, making them attractive candidates for the treatment of obesity and related metabolic disorders. Despite their potential, the number of TAAR1-targeting compounds with well-defined pharmacological profiles remains limited. In this study, we identified and characterized JP-14, a novel aminoguanidine-based TAAR1 agonist, in a comprehensive panel of pharmacological assays. JP-14 promoted glucose uptake in HepG2 cells and reduced lipid deposition during 3T3-L1 adipocyte differentiation, with both actions dependent on TAAR1 signaling. In differentiated 3T3-L1 adipocytes, JP-14 reduced intracellular levels of both neutral lipids and phospholipids, indicating dual anti-steatotic and anti-phospholipidotic activity. In zebrafish larvae, toxicity profiling confirmed 10 µg/mL as a safe concentration for further in vivo studies. These assays showed that JP-14 promoted lipid mobilization and partially prevented fructose-induced lipid accumulation, demonstrating systemic metabolic benefits in vivo. Moreover, JP-14 markedly delayed gastric emptying in mice, an effect similar to loperamide and reversed by TAAR1 antagonism, supporting its role in regulating satiety and energy balance. Collectively, our findings establish JP-14 as a safe and metabolically active TAAR1 agonist with multifaceted effects on glucose and lipid metabolism. JP-14 represents a valuable pharmacological tool for probing TAAR1-mediated mechanisms in metabolic regulation. Full article

Hydrogen serves as a key clean-energy carrier, with the main hurdles lying in safe, efficient transport and storage (gas or liquid) and in end-use energy conversion. Liquid hydrogen (LH), as a high-density method of storage and transportation, presents cryogenic insulation as its key technical issues. In LH storage tanks, the performance of high vacuum multilayer insulation (HVMLI) will decline due to hydrogen release and leakage from the microscopic pores of steel, which significantly destroy the vacuum layer. The accumulation of residual gases will accelerate thermal failure, shorten the service life of storage tanks and increase safety risks. Adsorption is the most effective strategy for removing residual gases. This review aims to elucidate materials, methods, and design approaches related to hydrogen storage. First, it summarizes adsorbents used in liquid hydrogen storage tanks, including cryogenic adsorbents, metal oxides, zeolite molecular sieves, and non-volatile compounds. Second, it explores experimental testing methods and applications of hydrogen adsorbents in storage tanks, analyzing key challenges faced in practical applications and corresponding countermeasures. Finally, it proposes research prospects for exploring novel adsorbents and developing integrated systems. Full article

The aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor, plays a crucial role in regulating immune homeostasis, inflammatory responses, and intestinal barrier function. Indole compounds and their derivatives are ligands of AHR, which can activate the AHR signal transduction pathway and show significant regulatory potential in various inflammatory and immune diseases. Cystic fibrosis (CF) is a life-threatening autosomal recessive genetic disorder. Cystic fibrosis transmembrane conductance regulator (CFTR) dysfunction affects multiple systems throughout the body. The core of its pathological process is chronic infection, abnormal inflammation, and tissue damage caused by mucus accumulation. Exploring alternative or adjunctive therapeutic strategies targeting pathological pathways downstream of CFTR is of significant importance. The aim of the present study is to explore the multiple beneficial effects that indole compounds may exert in regulating pulmonary infection and inflammation, repairing intestinal barrier function, and regulating immune homeostasis in CF patients by activating the AHR signaling pathway. Additionally, this study discusses the risks and challenges associated with developing indole compounds as CF drugs, offering a novel research approach distinct from traditional CFTR modulators for creating new CF therapeutics. Full article

Grammatophyllum speciosum Blume is an endangered wild orchid with medicinal properties. In this research, we propagated G. speciosum from vegetative organs grown under aseptic conditions. Subsequently, salinity stress was applied at the plantlet stage to investigate its effect on the accumulation of bioactive compounds. Half-strength Murashige and Skoog (½ MS) medium supplemented with a combination of 1 mg of L⁻¹ 1-naphthaleneacetic acid (NAA) and 0.5 mg of L⁻¹ 6-benzylaminopurine (BAP) proved to be a more suitable medium for shoot formation (32.33 ± 2.52 shoots per explant). The protocorm-like bodies, derived from embryogenic callus, were transferred into a temporary immersion bioreactor (TIB) system; 10-min of immersion every 3 h enhanced the maximum number of shoots, shoot height, and the fresh growth index (127.00 ± 2.16, 5.00 ± 0.51 cm and 4.26 ± 0.52, respectively). The proliferated plantlets from the TIB system successfully rooted in Vacin and Went medium. Furthermore, the plantlets were maintained in ½ MS medium supplemented with sodium chloride (NaCl) (0, 50, 100 or 200 µM) under a white light-emitting diode for 72 h to determine the total phenolic content (TPC) in the in vitro cultures. The TPC was highest in the medium with 100 µM of NaCl (111.06 ± 2.24 mg gallic acid equivalent g⁻¹ dry weight), the diphenyl picrylhydrazyl antioxidant activity was 24.50 ± 0.76% and ferric-reducing antioxidant power values were in the range 2441.79 ± 1.21 to 2491.96 ± 3.23 µM ascorbic acid equivalent g⁻¹ dry weight. The G. speciosum extracts showed antibacterial activity against acne pathogens, with minimum inhibitory concentration and minimum bactericidal concentration values in the ranges 6.4–12.8 mg mL⁻¹ and 12.8–25.6 mg mL⁻¹, respectively. Full article

Background: Acute lung injury (ALI) and its severe form, acute respiratory distress syndrome (ARDS), remain major causes of morbidity and mortality, yet no targeted pharmacological therapy is available. Excessive neutrophil and macrophage infiltration drives reactive oxygen species (ROS) production and cytokine release, leading to alveolar–capillary barrier disruption and fatal respiratory failure. Methods: We applied an integrative multi-omics strategy combining single-cell transcriptomics, peripheral blood proteomics, and lung tissue proteomics in a lipopolysaccharide (LPS, 10 mg/kg)-induced mouse ALI model to identify key signaling pathways. Harpagide, an iridoid glycoside identified from our natural compound screen, was evaluated in vivo (40 and 80 mg/kg) and in vitro (0.1–1 mg/mL). Histopathology, oxidative stress markers (SOD, GSH, and MDA), cytokine levels (IL-6 and IL-1β), and signaling proteins (HIF-1α, p-PI3K, p-AKT, Nrf2, and HO-1) were quantitatively assessed. Direct target engagement was probed using surface plasmon resonance (SPR), the cellular thermal shift assay (CETSA), and 100 ns molecular dynamics (MD) simulations. Results: Multi-omics profiling revealed robust activation of HIF-1, PI3K/AKT, and glutathione-metabolism pathways following the LPS challenge, with HIF-1α, VEGFA, and AKT as core regulators. Harpagide treatment significantly reduced lung injury scores by ~45% (p < 0.01), collagen deposition by ~50%, and ROS accumulation by >60% relative to LPS (n = 6). The pro-inflammatory cytokines IL-6 and IL-1β were reduced by 55–70% at the protein level (p < 0.01). Harpagide dose-dependently suppressed HIF-1α and p-AKT expression while enhancing Nrf2 and HO-1 levels (p < 0.05). SPR confirmed direct binding of Harpagide to HIF-1α (KD = 8.73 µM), and the CETSA demonstrated enhanced thermal stability of HIF-1α. MD simulations revealed a stable binding conformation within the inhibitory/C-TAD region after 50 ns. Conclusions: This study reveals convergent immune–microenvironmental regulatory mechanisms across cellular and tissue levels in ALI and demonstrates the protective effects of Harpagide through multi-pathway modulation. These findings offer new insights into the pathogenesis of ALI and support the development of “one-drug, multilayer co-regulation” strategies for systemic inflammatory diseases. Full article

This study established an efficient in vitro regeneration system using stem nodes from root collar suckers as explants. Subsequently, regenerated shoots were used to establish an in vitro medicinal production protocol that achieved ginkgolic acid production. The self-developed Ginkgo biloba medium (GBM), first reported in this study, was pivotal to system establishment. The plantlet propagation system showed that the bases of stem nodes dipped in GBM with 2 mg·L⁻¹ 6-benzyladenine (BA) and 0.2 mg·L⁻¹ 1-naphthaleneacetic acid (NAA) achieved near-complete axillary bud induction (99.56%). Adventitious shoot induction reached 82.22% (3.5 shoots/explant) using GBM with 0.2 mg·L⁻¹ BA, 0.02 mg·L⁻¹ kinetin (Kin) and 0.2 g·L⁻¹ proline (Pro). Maximum adventitious shoot elongation (92.22%, average 3.35 cm) was observed on GBM containing 0.1 mg·L⁻¹ zeatin (ZT) and 0.01 mg·L⁻¹ BA. After 3-week preculture with 15 mg·L⁻¹ phloroglucinol (PG), treatment with 0.6 mg·L⁻¹ indole-3-butyric acid (IBA) and 0.2% activated carbon (AC) yielded 96.67% rooting (6.19 roots/explant) and 85% acclimatization survival. For medicinal resource production, bud cluster induction at 94.44% (20.89 buds/explant) on GBM with 1 mg·L⁻¹ BA, 0.03 mg·L⁻¹ Kin, and 0.2 g·L⁻¹ Pro. Leaf organs in GBM with 0.3 mg·L⁻¹ BA, 0.01 mg·L⁻¹ Kin, 0.01 mg·L⁻¹ IBA, 0.3 g·L⁻¹ Pro, and 0.01 mg·L⁻¹ glutamine (Gln) accumulated 20.64 g fresh weight and 41.910 mg·g⁻¹ DW ginkgolic acids, representing a 4.93-fold increase over mother plants. This system enables large-scale Ginkgo biloba L. propagation and provides an in vitro strategy for producing medicinal compounds in endangered plants. Full article

Aronia melanocarpa is rich in anthocyanins, compounds with significant medicinal and industrial value, making it an attractive species for enhanced production. Compared with fruits or intact plants, callus tissue offers a uniform, controllable in vitro system that is particularly suitable for dissecting regulatory mechanisms under defined environmental conditions. Although light quality is known to influence anthocyanin biosynthesis, its specific regulatory mechanisms in A. melanocarpa remain unclear. In this study, callus tissues were cultured under six light regimes: full-spectrum LED, blue:red (5:1), red:blue (5:1), red:blue:white (1:1:1), red:white (5:1), and pure blue light. Anthocyanin content was quantified using the pH differential method, and the results showed that the blue:red (5:1) treatment produced the highest accumulation, reaching 14.06 mg/100 g. Transcriptome sequencing was then performed to compare the gene expression profiles between calli cultured under blue:red (5:1) light and those maintained in darkness. A total of 10,547 differentially expressed genes (DEGs) were identified, including 6134 upregulated and 4413 downregulated genes. Functional enrichment analysis indicated that these DEGs were mainly involved in anthocyanin biosynthesis and transport. Importantly, key structural genes such as PAL, C4H, 4CL, CHS, ANS, UFGT, and GST were significantly upregulated under blue:red (5:1) light, as further validated by qRT-PCR. Overall, our findings demonstrate that a blue:red (5:1) light ratio enhances anthocyanin accumulation by promoting the expression of biosynthetic and transport-related genes. This study not only provides new transcriptomic insights into the light-mediated regulation of secondary metabolism in A. melanocarpa callus, but also establishes a foundation for optimizing in vitro culture systems for sustainable anthocyanin production. Full article

In arid areas, the combined use of plastic sheeting under gravel-sand mulch on ridge-furrow planting systems is an emerging practice to minimize soil water evaporation and micro-plastic pollution. In this study, we conducted a two-year field experiment near Gobi-Tengger Desert in Ningxia, China, to evaluate the effects of a plastic film underneath a layer of pure sand (MS1), pure gravel (MS2) and mixed gravel-and-sand (MS3) mulch on the soil hydrothermal properties, water use efficiency, yield, and fruit quality of wolfberry, compared to bare soil (CK). The results showed that mulching significantly increased soil temperature and water content in the 0–20 cm surface layer, though the effects varied with soil depth and water availability between a supplemental irrigated year (2022) and a rain-fed year (2023). Mulching markedly altered soil water dynamics, enhancing the capture and retention of light-to-heavy rainfall events. Consequently, all mulches significantly increased seasonal water consumption (ET) and water use efficiency (WUE) compared to CK. The MS1 treatment consistently achieved the highest yield and WUE, and the highest accumulation of beneficial fruit compounds like polysaccharides and flavonoids. However, this treatment also resulted in elevated soil salinity. Our findings demonstrate that combined mulching, especially MS1, is a highly effective strategy for optimizing soil conditions, water productivity, and fruit quality in wolfberry cultivation, although long-term salinity management requires attention. Full article

The nanoencapsulation of bioactive compounds such as β-carotene and microalgal extracts has emerged as an effective strategy to enhance their stability, bioavailability, and biological efficacy, particularly against oxidative stress. Dunaliella tertiolecta, a microalga rich in carotenoids and chlorophylls, presents notable antioxidant and erythroprotective properties; however, its bioactive potential is limited by low bioaccessibility and degradation during processing and digestion. This study aimed to develop and evaluate nanoliposomes loaded with D. tertiolecta extract and β-carotene as sustained-release systems to improve antioxidant performance and erythroprotective effects. The methodology involved optimizing microalgal cultivation under nitrogen and salinity stress to enhance pigment accumulation, followed by extraction, nanoencapsulation via the particle dispersion method, and physicochemical characterization of the nanoliposomes. Antioxidant capacity and release kinetics were assessed through ABTS and FRAP assays, while erythroprotective activity was evaluated by monitoring oxidative hemolysis in human erythrocytes. The release kinetics revealed an anomalous transport mechanism for both systems, with β-carotene showing faster and more efficient release due to its greater lipophilic compatibility with the nanoliposomal matrix. The nanoliposomal systems demonstrated nanoscale size, high encapsulation efficiency, sustained antioxidant release, and effective erythrocyte protection, with the extract-loaded formulation exhibiting synergistic effects superior to isolated β-carotene. These findings support the potential application of this nanotechnology-based delivery system in functional foods, nutraceuticals, and biomedical formulations aimed at preventing oxidative stress-related cellular damage. Full article

Phosphorus (P) deficiency and soil-borne fungal diseases are major constraints to peanut (Arachis hypogaea L.) production. Phosphate-solubilizing bacteria (PSB) can improve P availability in the soil, thereby promoting plant growth. However, their potential to improve plant resistance against pathogens under P-limited conditions remains poorly understood. In this study, we first evaluated the ability of two PSB strains, Enterobacter sp. J49 and Serratia sp. S119, to induce systemic resistance (ISR) in peanut plants against the fungal pathogen Sclerotium rolfsii. Results showed that strain S119 reduced disease severity by 40%, whereas strain J49 reduced both incidence (30%) and severity (40%). The protective effect produced by strain J49 was mediated by ISR, as evidenced by the early increase in phenolic compounds accumulation (48 h) and total peroxidase activity (72 h) in inoculated plants. Under P-deficient conditions, the J49 strain was also able to protect peanut plants against S. rolfsii, as demonstrated by a significant reduction in disease severity (55%). These findings highlight the potential of multifunctional bacterium Enterobacter sp. J49 to enhance sustainable peanut production by simultaneously improving P acquisition and strengthening plant defense mechanisms. Full article