Search Results (68)

The cold temperate forest ecosystem is a crucial ecological zone in China, significantly impacted by human activities. To understand the impact of restoration on soil microbial communities following disturbance, this study employed high-throughput sequencing technology to systematically examine the assembly patterns and processes of soil microbial communities under two restoration modes (nature restoration (NR) and artificial restoration (AR)) in this forest ecosystem. The results indicated that the concentrations of total nitrogen (TN), alkaline hydrolysable nitrogen (AN), dissolved organic carbon (DOC) and soil organic carbon (SOC) were significantly higher in soils under natural restoration compared to artificial restoration. The α-diversity of soil bacteria remained unchanged, while soil fungal α-diversity changed significantly across different restoration modes. Furthermore, different restoration modes significantly alter the β-diversity of soil microbial (bacterial and fungal) communities. The relative abundance of soil microbial (bacterial and fungal) changed significantly across different forest restoration strategies, i.e., the relative abundance of Pajaroellobacter increased in natural restoration compared to that in natural forest; similarly, both Podila and Russula showed higher relative abundances in natural restoration than those in natural forest. Furthermore, analysis of variance for differences between groups shows that Incoybe plays a crucial role in artificial restoration. Community assembly analyses indicated that that soil microbial (bacterial and fungal) communities were primarily driven by deterministic processes in both restoration models. In short, our study improves our comprehension of how soil microbial communities respond to different restoration methods in temperate forest ecosystems, providing valuable insights for their sustainable management. Full article

Soil microorganisms are crucial in global biogeochemical cycles, impacting ecosystems’ energy flows and material cycling. This study, via high-throughput sequencing in four forests—the original Larix gmelinii (Rupr.) Kuzen. forest (LG), the conifer–broad-leaved mixed Pinus sylvestris var. mongolica Litv. forest (PS), the original pure Betula platyphylla Sukaczev forest (BP), and the original pure Populus L. forest (PL) in Shuanghe National Nature Reserve, Daxing’anling mountains—explored soil microbial community structures and diversities. The results indicated that the BP and PL forests had the lowest soil bacterial ACE and Chao1 indices, and the original pure birch forest’s Shannon index was higher than that of the poplar forest. The soil’s fungal Chao1 index of the birch forest was higher than that of the larch forests. Bradyrhizobium and Roseiarcus were the dominant soil bacterial genera; the dominant soil fungal genera were Podila, Russula, and Sebacina. RDA and mantel analyses indicated that soil microbial community structures varied across forest types mainly because of the effective phosphorous and pH levels, soil’s total nitrogen level, and available phosphorus level. This study offers a scientific foundation for cold-temperate-forest ecosystem management regarding soil microbial diversity and community structural changes in different forest types. Full article

The long-term post-fire recovery phase is a critical stage for forest ecosystems to progress toward regeneration and mature succession. During this process, soil bacteria exhibit greater environmental adaptability, rapidly driving nutrient cycling and facilitating vegetation restoration. This study investigated the community structure and diversity of soil bacteria during long-term recovery after forest fires in the cold temperate zone, focusing on soils from the 2000 fires in Daxing’anling. Soil samples were classified into Low (L), Moderate (M), and High (H) fire damage intensity, with bacterial community composition and diversity analyzed using Illumina sequencing technology. After long-term fire recovery, the contents of soil organic carbon, black carbon, total nitrogen, alkaline nitrogen, available phosphorus, and available potassium were significantly higher elevated (p < 0.05), and water content was significantly lower, compared with that in the control check (CK) group. Soil urease, fluorescein diacetate, soil acid phosphatase, and soil dehydrogenase activities were significantly higher, and soil sucrase activity was significantly lower in H. There was a significant difference in the Alpha diversity index among the groups. Compared with CK, the Shannon index was significantly increased (p < 0.05) in L, while both Chao1 and Shannon indices were significantly decreased (p < 0.05) in M and significantly higher in H than CK. The results of the PCoA showed that there was a significant difference in the Beta diversity of the bacterial community among the groups (R² = 0.60 p = 0.001). The dominant bacteria groups were Proteobacteria and Acidobacteriota, while Actinobacteria became the new dominant group during the long-term post-fire recovery. AP, WC, DOC, MBC, S-DHA, and S-SC were significantly and positively correlated with soil bacterial diversity (p < 0.05). The results of the co-occurrence network analysis showed that all groups were dominated by symbiotic relationships, with M having the highest network complexity and strongest competitive effects. This study found that the physicochemical properties of soils recovered over a long period of time after fire returned to or exceeded the unfired forest condition. The Actinobacteria phylum became a new dominant bacterial group, with stronger network complexity and competition, in the process of forest recovery after moderate fire. Full article

Phytolith carbon sequestration has been recognized as an important mechanism for long-term carbon sequestration in forest ecosystems. Conducting relevant research in cold temperate regions that are sensitive to climate change can reveal their unique mechanisms as a stable and long-term carbon pool, fill key blind spots in global carbon cycling models, and provide necessary scientific support for developing climate-resilient ecological strategies and carbon neutrality pathways. In this study, we focused on the Larix gmelinii forest ecosystem and investigated the latitudinal spatial characteristics of soil phytolith and phytolith-occluded carbon (phytOC) in Eastern China. We analyzed the factors that influenced their accumulation and assessed their storage potential across different climatic zones. Our findings revealed an exponential increase in soil phytolith content with increasing latitude in Eastern China. Additionally, the content of soil phytoliths in tropical and subtropical forests was significantly lower than in the cold temperate forests. It was also found that soil phytOC content increased linearly with latitude and was significantly higher in cold temperate zones than in the other climatic zones. The order of soil phytOC storage was tropical (0.23 t ha⁻¹) < middle temperate (0.24 t ha⁻¹) < subtropical (0.27 t ha⁻¹) < cold temperate (1.20 t ha⁻¹). Soil phytolith and phytOC content were significantly negatively correlated with temperature and precipitation. pH, organic matter, and nutrients of soil significantly influenced the formation and accumulation of soil phytoliths. It can provide a scientific basis for the quantitative evaluation of forest soil carbon pool. Full article

Larch shoot blight, caused by the fungus Neofusicoccum laricinum, threatens larch (Larix spp.) forests across northeastern China, jeopardizing both timber productivity and ecological stability. This study aimed to investigate the genomic diversity, population structure, and potential adaptive mechanisms of N. laricinum across contrasting climatic regions. To achieve this, we conducted whole-genome resequencing of 23 N. laricinum isolates collected from three major provinces—Heilongjiang, Inner Mongolia, and Jilin—that represent distinct climatic zones ranging from cold-temperate to relatively warmer regions. We identified ~219.1 K genetic variants, offering a detailed portrait of the pathogen’s genomic diversity. Population structure analyses, including principal component analysis and phylogenetic tree, revealed clear genetic differentiation aligning with geographic origin and climate. Functional annotation (GO and KEGG) highlighted enrichment in metabolic, stress-response, and membrane transport pathways, suggesting potential adaptation to varied temperature regimes and environmental pressures. Moreover, region-specific variants—particularly missense and stop-gain mutations—were linked to genes involved in ATP binding, oxidoreductase activity, and cell division, underscoring the fungus’s capacity for rapid adaptation. Collectively, these findings fill a critical gap in the population genetics of N. laricinum and lay a foundation for future disease management strategies to larch shoot blight under changing climatic conditions. Full article

The effects of climate change are particularly pronounced in cities, where urban green infrastructure—such as trees, parks, and green spaces—plays a vital role in both climate adaptation and mitigation. This study assesses the carbon sequestration potential of urban forests in Budapest, the capital city of Hungary, which lies at the intersection of the Great Hungarian Plain and the Buda Hills, and is traversed by the Danube River. The city is characterized by a temperate climate with hot summers and cold winters, and a diverse range of soil types, including shallow Leptosols and Cambisols in the limestone and dolomite hills of Buda, well-developed Luvisols and Regosols in the valleys, Fluvisols and Arenosols in the flood-affected areas of Pest, and Technosols found on both sides of the city. The assessment utilizes data from the National Forestry Database and the Copernicus Land Monitoring Service High Resolution Layer Tree Cover Density. The results show that Budapest’s urban forests and trees contribute an estimated annual carbon offset of −41,338 tCO₂, approximately 1% of the city’s total emissions. The urban forests on the Buda and Pest sides of the city exhibit notable differences in carbon sequestration and storage, age class structure, tree species composition, and naturalness. On the Buda side, older semi-natural forests dominated by native species primarily act as in situ carbon reservoirs, with limited additional sequestration capacity due to their older age, slower growth, and longer rotation periods. In contrast, the Pest-side forests, which are primarily extensively managed introduced forests and tree plantations, contain a higher proportion of non-native species such as black locust (Robinia pseudoacacia) and hybrid poplars (Populus × euramericana). Despite harsher climatic conditions, Pest-side forests perform better in carbon sink capacity compared to those on the Buda side, as they are younger, with lower carbon stocks but higher sequestration rates. Our findings provide valuable insights for the development of climate-resilient urban forestry and planning strategies, emphasizing the importance of enhancing the long-term carbon sequestration potential of urban forests. Full article

Pyrogenic carbon (PyC) is generated from the incomplete combustion of biomass and fossil fuels. Pyrogenic carbon is highly stable and is often referred to as a missing carbon sink. It plays a crucial role in global carbon cycling and climate change research. We analyzed the storage of PyC and uncharred biological organic carbon (BOC) within woody debris (WD) and the charcoal layer, as well as the properties of PyC, across four forest types in the cold temperate coniferous forest of the Greater Khingan Mountains. Pyrogenic carbon in WD appears as charred, blackened material, while PyC in the charcoal layer was extracted through chemical oxidation using HF/HCl treatment. Our methodology included particle size separation through dry sieving, followed by the analysis of four size fractions (>2 mm, 2–1 mm, 1–0.5 mm and <0.5 mm) for elemental composition, and the chemical composition was analyzed using DRIFT. With respect to WD, PyC storage ranged from 0.040 to 0.179 Mg·ha⁻¹, whereas BOC storage ranged from 3.1 to 16.8 Mg·ha⁻¹. In the charcoal layer, PyC storage ranged from 7.9 to 44.3 Mg·ha⁻¹, and BOC storage ranged from 3.8 to 11.6 Mg·ha⁻¹. Pyrogenic carbon storage in the charcoal layer dominated (>99%) on the above-ground in each forest type. The DRIFT analysis confirmed that the coarse fraction (>2 mm) contain more polymeric aromatic structures, and most likely indicated the presence of benzene carboxylic compounds (1710 cm⁻¹), which may originate from the charred plant material. Our research aims to enhance the understanding of the retention effects of recalcitrant carbon in WD and charcoal layer of cold temperate coniferous forest, thereby providing new insights into the impact of fire disturbances on carbon cycling within forest ecosystems. Full article

Biomass combustion produces between 50 and 270 Tg of pyrogenic carbon (PyC) annually. PyC is extremely highly stable, making it a significant component of the global carbon sink. We established four plots at different slope positions within a cold temperate coniferous forest that experienced a severe fire in 2010. We mechanically divided the soil into three depths. The PyC content and density of the collected soil samples and four particle sizes were analyzed. Thirteen years after the fire, the PyC content in the soil on the upper slope was low (13.5–14.2 g·kg⁻¹). In terms of PyC density, the valley and the upper slopes presented lower values. The PyC content in the 0~10 cm layer (14.0–16.7 g·kg⁻¹) is only slightly more than 20% higher than that in the two deeper layers, whereas its density is 1.5~2 times more than that in the other layers. Our findings indicate that PyC is predominantly concentrated in coarse sand and silt particles. The spatial pattern of PyC is significantly influenced by the differentiation in topography, soil layer depth, and particle size. These distribution patterns strongly show that PyC plays a key role in forest ecosystem cycles affected by fire. PyC distribution in particle sizes particularly shows connections with specific soil components. There is a synergistic effect between the topographic redistribution (slope position differences), vertical stratification (soil depth), and particle size sorting of PyC. This determines the retention effect of stable carbon in fire-disturbed forest ecosystem soils, thereby influencing the soil carbon cycle. Full article

Carbon (C), nitrogen (N), and phosphorus (P) are vital nutrients in the soil, exerting a profound influence on the primary productivity of ecosystems. However, our understanding of how the understory influences soil nutrients and their stoichiometry remains limited, especially in cold-temperate forests where the understory plays a crucial role in mediating soil nutrient cycling. To elucidate the effect of understory vegetation on soil nutrients, three typical larch forests, namely Sphagnum–Bryum–Rhododendron tomentosum–Larix gmelinii forest (SLL), Rhododendron dauricum–Larix gmelinii forest (RL), and Rhododendron tomentosum–Larix gmelinii forest (LL), were selected in the typical cold-temperate region of northeast China to determine the soil organic carbon (SOC), total nitrogen (TN), total phosphorus (TP) contents, and their stoichiometric characteristics in 0–100 cm soil depth. The results revealed the following: (1) Significant differences in soil nutrient and its stoichiometry existed among the three different forest types (p < 0.001), with the SLL displaying the highest mean SOC, TN, and TP contents, as well as soil C:N, C:P, and N:P ratios, whereas the RL exhibited the lowest values (p < 0.05). (2) Across the 0–100 cm soil profile, the soil nutrient content and stoichiometry showed decreasing trends with soil depth, with significant differences among the soil layers. (3) Variations in soil stoichiometry were significantly correlated with soil bulk density, pH, soil temperature, soil water content, total porosity, and capillary porosity (p < 0.05). This study underscores the necessity of further consideration of the impact of understory vegetation in future research on soil stoichiometry in forest ecosystems. Full article

Vegetation and subsequent ecosystem services can recover over time in forest headwaters devastated by massive disasters. However, in cold regions, their recovery rates are typically slow and often imperceptible, which makes it difficult to evaluate how much ecosystem services have recovered. This study targeted dissolved organic matter (DOM), which plays a central role in biogeochemical processes in forest ecosystems, and aimed to examine whether vegetation conditions affect the quality of stream DOM from cool-temperate forest headwaters in northern Japan. To achieve this, hydrological observations and stream water sampling were conducted monthly from May to December 2021 in three small forest catchments with different landslide coverage. Dissolved organic carbon (DOC) concentration in stream water was measured, and the molecular composition of DOM was analyzed using ultrahigh-resolution mass spectrometry and compared among the three catchments. The peak-intensity-weighted average aromaticity index (AI_wa) increased with DOC concentration. We found that AI_wa was the highest in the undisturbed catchment, followed by the catchments with landslide coverages of 16% and 52% at a given DOC level. These results indicate that the quality of DOM could dramatically change depending not only on DOC concentration but also on vegetation disturbance in cool-temperate forest headwaters. Full article

Vegetation phenology has attracted considerable attention as one of the most sensitive indicators of global climate change. Remote sensing has significantly expanded our understanding of the spatial divergences of vegetation phenology. However, the current understanding of the reasons behind spatial divergences of vegetation phenology is not yet complete, and there is an urgent need to unravel the landscape processes driving spatial divergences of vegetation phenology. In light of this, the present study focused on montane forests of the cold temperate zone as its study area, collecting datasets such as the MCD12Q2 land surface phenology product, climate, topography, and stand height and adopting regression analysis and geo-detector model to investigate the individual and interactive effects of variables such as temperature, precipitation, elevation, slope, aspect, and forest height on forest phenology. The results indicated that because of the complexity of topography, the impacts of temperature on forest phenology were nonlinear. With fluctuation of elevation, the development of forest occurred later at the base and ridges of mountain and earlier in the valley bottom lands and mid-upper slopes. Temperature and precipitation exhibited a bilaterally strong interactive effect with slope on forest greenup. Both forest greenup and dormancy occurred earlier on shady slopes and later on sunny slopes. There may also exist an interactive effect between forest height and topographic factors on the spatial divergences of forest phenology. Future research may need to focus on whether there is a trade-off or synergy between the macroclimatic regulatory function of topography and the microclimatic regulatory function of canopy structure. Full article

The leaf area index (LAI) in temperate forests is highly dynamic throughout the season, and lacking such dynamic information has limited our understanding of carbon and water flux patterns in these ecosystems. This study aims to explore the potential of using vegetation indices based on Sentinel-2 data, which includes three additional spectral bands in the red-edge region of its multispectral imager (MSI) sensor compared to previous satellite-borne imagery, to effectively track seasonal variations in LAI within typical cold–temperate deciduous forests originating in rugged terrain in Japan. We evaluated reported vegetation indices and developed an index specific to Sentinel-2 data to effectively monitor the spatiotemporal changes of LAI in mountainous deciduous forests, providing more accurate data for ecological monitoring. Results showed that the developed index (SR_B12,B7) was able to track LAI at both seasonal and spatial scales (R² = 0.576). Further analyses revealed that the index nevertheless performed relatively poorly during the leaf-maturing season when LAI peaks, suggesting that it still suffers from a “saturation” problem. For high-resolution tracking of LAI in temperate deciduous forests at both temporal and spatial scales, future research is needed to incorporate additional information. Full article

The presence of shade-tolerant tree invaders has been recently noted in tropical and temperate forest understories. Maximum growth rate is an important trait for exotic trees becoming invaders in a forest. This study aimed to determine the growth rate of Eriobotrya japonica in a secondary cloud forest in central Veracruz, Mexico. The objectives of this study were to determine wood density, tree ring boundaries and number, and their relationship to diameter at breast height (DBH) and climate data. Tree ring counts were obtained using Python-based software with subsequent visual validation. Growth rates were measured using diametric tape, dendrometric bands, and the pinning method. The number of rings increased with DBH, presenting values ranging from 14 to 27. Tree rings were delimited by fibers that were five times narrower in the ring limit zone than in the intra-ring zone. The growth ring delimitation zones were formed when vascular cambium activity stalled during the relatively dry-cold season (January–February). The growth rate of E. japonica was statistically similar (ca. 0.2 mm yr⁻¹) regardless of the method employed to measure it. Relative growth rate was low (0.02 cm cm⁻¹ yr⁻¹). Wood density (0.76 g cm⁻³) values lay within the upper values for mature forest trees. Eriobotrya japonica is a potential invader, with traits such as high wood density and a relatively low growth rate, which are characteristic of the shade-tolerant tree species. Full article

In boreal and temperate forests, symbiotroph and saprotroph soil fungi must survive months of low temperatures or freezing during winter. In the temperate biome, this is particularly the case for high-elevation mountain forests. Soil freezing is thus an important stress factor in these forests. The objective of this study was to assess how temperature and freezing conditions affect the growth and survival of symbiotic and saprotrophic fungi. To assess the cold and freezing tolerance of ectomycorrhizal (EM) and saprotrophic (SAP) fungi, we conducted a study from 2021 to 2023, using isolates from forests located at lower and high-elevation mountain sites, as well as from forests in Mongolia, at altitudes ranging from 525 m to 1800 m. The isolates were grown in vitro at temperatures of 22, 15, and 4 °C and exposed to freezing conditions at −4 or −18 °C. The response to temperature and freezing was determined based on radial growth. Triphenyltetrazolium chloride (TTC) reduction was used to measure relative metabolic activity and viability. Fungi that originated from higher-elevation mountain sites, and thus colder climate conditions, tended to have a lower response to temperature and a higher tolerance to freezing. We could find no evidence of a higher freezing tolerance among different exploration types of ectomycorrhizal fungi. Sensitivity to low temperatures appears to be taxa-specific rather than exploration-type-specific. Full article

We evaluated the long-term impacts of various forest management practices on the structure and biodiversity of Estonian hemiboreal forests, a unique ecological transition zone between temperate and boreal forests, found primarily in regions with cold winters and moderately warm summers, such as the northern parts of Europe, Asia, and North America. The study examined 150 plots across stands of different ages (65–177 years), including commercial forests and Natura 2000 habitat 9010* “Western Taiga”. These plots varied in stand origin—multi-aged (trees of varying ages) versus even-aged (uniform tree ages), management history—historical (practices before the 1990s) and recent (post-1990s practices), and conservation status—protected forests (e.g., Natura 2000 areas) and commercial forests focused on timber production. Data on forest structure, including canopy tree diameters, deadwood volumes, and species richness, were collected alongside detailed field surveys of vascular plants and bryophytes. Management histories were assessed using historical maps and records. Statistical analyses, including General Linear Mixed Models (GLMMs), Multi-Response Permutation Procedures (MRPP), and Indicator Species Analysis (ISA), were used to evaluate the effects of origin, management history, and conservation status on forest structure and species composition. Results indicated that multi-aged origin forests had significantly higher canopy tree diameters and deadwood volumes compared to even-aged origin stands, highlighting the benefits of varied-age management for structural diversity. Historically managed forests showed increased tree species richness, but lower deadwood volumes, suggesting a biodiversity–structure trade-off. Recent management, however, negatively impacted both deadwood volume and understory diversity, reflecting short-term forestry consequences. Protected areas exhibited higher deadwood volumes and bryophyte richness compared to commercial forests, indicating a small yet persistent effect of conservation strategies in sustaining forest complexity and biodiversity. Indicator species analysis identified specific vascular plants and bryophytes as markers of long-term management impacts. These findings highlight the ecological significance of integrating historical legacies and conservation priorities into modern management to support forest resilience and biodiversity. Full article