Search Results (1,590)

Osmotic stress limits rice productivity, yet the crosstalk between hydrogen sulfide signaling and redox regulation remains incompletely understood. We previously showed that redox-dependent oligomerization of the basic (region) leucine zippers transcription factor bZIP68 at Cys245 confers osmotic tolerance. However, the role of an adjacent cysteine, Cys171, was undefined. Here, we demonstrate that osmotic stress induces persulfidation of bZIP68 specifically at Cys171. This modification facilitates Cys245-mediated oxidation-dependent oligomerization, thereby enhancing bZIP68 transcriptional activity toward COLD-REGULATED413-THYLAKOID MEMBRANE1 (COR413-TM1). Transgenic complementation and physiological assays confirmed that Cys171 persulfidation is essential for full bZIP68 function in osmotic adaptation. Transcriptomic analysis further revealed that Cys171 is required for bZIP68-driven transcriptional reprogramming under stress. Our findings establish a hierarchical redox cascade wherein persulfidation primes bZIP68 for oxidative activation, highlighting a regulatory crosstalk between distinct post-translational modifications. These mechanistic insights expand our understanding of H₂S signaling and identify the bZIP68 cysteine network as a potential target for improving crop stress resilience. Full article

Hippophae tibetana Schltdl. is a cold-tolerant deciduous shrub endemic to the Tibetan Plateau, playing a vital ecological role in high-altitude environments. This study utilized the Biomod2 platform to model its current and future potential distribution under climate change, integrating 34 environmental variables across bioclimatic, topographic, edaphic, anthropogenic, and ultraviolet (UV) dimensions. Among ten candidate species distribution models (SDMs), the random forest (RF) algorithm exhibited the highest predictive accuracy and stability. An ensemble model (EM) combining RF, GBM, MARS, and FDA further improved predictive performance (ROC = 0.992, TSS = 0.923, and Kappa = 0.886). Key determinants of habitat suitability included altitude, temperature, UV radiation, and biodiversity, with RF response curves revealing distinct nonlinear thresholds. Optimal suitability occurred at around a 4000 m elevation, decreasing beyond this range, while temperature and UV exhibited similar unimodal responses. Under the SSP2-4.5 climate scenario, the suitable habitat is projected to expand from the 2050s to the 2090s, particularly in eastern Qinghai, southwestern Gansu, northwestern Sichuan, and central–southern Tibet. The species’ distribution centroid is anticipated to shift southwestward toward Qinghai Province, with more rapid migration projected after the 2050s. These findings underscore the complex interplay of environmental factors shaping H. tibetana distribution and offer valuable insights for conservation planning in the ecologically fragile Tibetan Plateau. Full article

In order to protect the high-sensitivity optical lens of the “magnetic field and velocity field imager” in extreme deep space environments, this paper proposes a new type of dual redundant planetary differential lens cover drive mechanism. In view of the critical vulnerability that traditional single-motor direct drive is prone to sudden mechanical jamming and catastrophic single-point failure (SPF) in severe tasks such as Jupiter exploration, this study constructs a “dual input single output (DISO)” rigid decoupling architecture from the perspective of physical topology. Through theoretical analysis and kinematic modeling, the adaptive decoupling mechanism of the two-degree-of-freedom (2-DOF) system under unilateral mechanical stalling is revealed. Dynamic analysis shows that in the nominal dual-motor synergy mode, the system shows a significant “kinematic load-sharing effect”, thus greatly reducing the sliding friction and gear wear rate. In addition, under the severe dynamic fault injection scenario (maximum gravity deviation and sudden jam superposition of a single motor), the cold standby motor is activated and the dynamic takeover is quickly performed. The high-fidelity transient simulation based on ADAMS verifies that although the fault will produce transient global torque spikes and pulsed internal gear contact forces at the moment, all extreme dynamic loads remain well within the structural safety margin. The output successfully achieved a smooth transition, which is characterized by a non-zero-crossing velocity recovery. This research provides an innovative theoretical basis and a practical engineering paradigm for the design of high-reliability fault-tolerant mechanisms in deep space exploration. Full article

Background: Post-stroke upper-limb spasticity can cause pain, hinder passive care, and lead to secondary musculoskeletal complications. Current minimally invasive treatments have important limitations. Cryoneurolysis, which creates a controlled cold lesion of peripheral nerves, may offer a partially reversible focal denervation alternative. Methods: We conducted a feasibility case series in the outpatient department of a rehabilitation centre. Three adults with chronic post-stroke hemiparesis and a non-functional spastic upper limb underwent ultrasound- and nerve stimulation-guided cryoneurolysis of the musculocutaneous, median, and/or ulnar nerves. All had demonstrated a positive response to diagnostic nerve blocks beforehand. Feasibility outcomes included completion of planned nerve targets, tolerability under local anesthesia, absence of serious adverse events, and completion of 6-month follow-up. Secondary outcomes were Modified Ashworth Scale (MAS), qualitatively assessed passive joint mobility (video-documented), pain measured by visual analogue scale, sensory testing, and electroneuromyography (ENMG). Results: All procedures were completed as planned. Treatment was well tolerated under local anesthesia, and no serious adverse events occurred. MAS decreased by at least 2 points in targeted patterns, with immediate improvement in passive mobility; these effects persisted at 6 months. Pain remained unchanged in two participants and improved in one. Sensory testing at 6 weeks was stable. ENMG findings were heterogeneous, including reduced ulnar sensory action potential amplitude and biceps denervation activity in one participant. Conclusions: In this small series, cryoneurolysis for post-stroke upper-limb spasticity was feasible and associated with sustained tone reduction and improved passive mobility. Larger controlled studies are required to better define safety, optimize targeting strategies, and assess patient-centred outcomes. Full article

Capsicum annuum is a Solanaceae crop that is sensitive to cold, which affects its growth and development upon prolonged exposure and ultimately reduces yield. In response, a complex regulatory network of cold-responsive genes is activated. Earlier studies have shown that SnRKs play a positive role in enhancing cold tolerance in different crops, including peppers; however, the underlying molecular mechanisms and downstream targets have yet to be fully elucidated. In this study, yeast hybrid screening using CaSnRK2.4 identified a potential interacting partner CaPDX1. The interaction between CaPDX1 and CaSnRK2.4 was further confirmed through Y2H, luciferase complementation, and bimolecular fluorescence complementation assays. Subcellular localization showed that CaPDX1 and CaSnRK2.4 are localized in the nucleus as well as in the cell membrane. Silencing of CaPDX1 through VIGS showed increased susceptibility of peppers to cold stress, negatively influenced antioxidant enzymatic activities, and increased relative electrolyte leakage and malondialdehyde levels. Conversely, transient overexpression of CaPDX1 in peppers enhanced cold tolerance by reducing the accumulation of REL and MDA. Ectopic expression of CaPDX1 in Arabidopsis thaliana significantly improved its cold tolerance, accompanied by enhanced activity of antioxidant enzymes and increased chlorophyll content. In summary, these results indicate that CaPDX1 is a positive regulator of cold tolerance in pepper, and its mechanism of action involves interaction with CaSnRK2.4 and the regulation of physiological and molecular responses in pepper under cold stress. Full article

Dwarf bananas are an important tropical fruit crop. They are particularly susceptible to cold stress, which often leads to quality deterioration. Although previous studies have examined the effects of cold stress on dwarf bananas, research on effective regulatory strategies and underlying mechanisms remains limited. This study investigates the mechanistic regulatory effects of chitosan (CTS) on cold stress in postharvest dwarf bananas, revealing that CTS mitigates cold-induced injury and improves fruit quality. Using an integrated approach of metabolomics, lipidomics, and enzyme activity assays, this study explored the potential mechanisms by which CTS alleviates chilling injury. Lipidomic results showed that CTS enhances cold tolerance by regulating the metabolism of glycerides, glycerophospholipids, linoleic acid, and linolenic acid. Metabolomics data further indicated that CTS increases the levels of amino acids, carbohydrates, and key substrates and intermediates of the tricarboxylic acid (TCA) cycle in cold-stressed dwarf bananas. Collectively, these effects enhance respiration, energy homeostasis, and antioxidant capacity, enabling dwarf bananas to better tolerate low-temperature stress. Full article

Pancreatic ductal adenocarcinoma (PDAC) remains one of the deadliest malignancies, characterized by early metastasis, dense desmoplastic stroma and a profoundly immunosuppressive, lymphocyte-depleted tumor microenvironment that severely limits the efficacy of current systemic and immunotherapeutic approaches. Oncolytic viruses (OVs), which selectively replicate in and lyse malignant cells while activating antitumor immunity, have emerged as attractive candidates to convert this “cold” tumor into a more inflamed and therapeutically responsive disease. In this review, we summarize clinical evidence on the main OV platforms evaluated in PDAC, including adenovirus, herpes simplex virus, vaccinia virus, parvovirus and reovirus, with a focus on clinical trials. Across these classes of viruses, intratumoral administration has consistently proven feasible and generally well tolerated, with frequent evidence of viral replication, microenvironmental remodeling and immune activation, but only modest and often transient antitumor responses in small, early-phase cohorts. We then discuss key biological and translational challenges that currently limit OV impact in PDAC, such as systemic delivery in the context of pre-existing antiviral immunity and rapid clearance, penetration through the fibrotic stroma, and rational selection of encoded transgenes to reshape myeloid cell-driven, pro-tumoral inflammation and enhance T-cell recruitment. Finally, we outline future directions for the field, including carrier-cell–based systemic delivery, stroma-targeting or cytokine-armed constructs, and combinatorial strategies with chemotherapy and immune checkpoint blockade, arguing that design refinement, innovative combinations and mechanism-driven trial designs will be essential to unlock the full therapeutic potential of oncolytic virotherapy in PDAC. Full article

The actinobacterial strain DEC002 was isolated recently from volcanic soils of Deception Island. Its taxonomic identity was resolved through a polyphasic strategy integrating morphology, physiological profiling, multilocus phylogeny, and genome-wide comparisons to resolve its identity. Concatenated core gene trees together with average nucleotide identity and digital DNA–DNA hybridization values place DEC002 within Actinacidiphila fildesensis with robust support. This is the first molecular confirmation of the species beyond King George Island and secures a second verified locality within the South Shetland Archipelago. Growth at low temperature with tolerance to moderate salinity indicates a psychrotolerant lifestyle. Cell-free supernatants inhibited representatives of foodborne Gram-negative and Gram-positive bacteria, including representatives of Enterobacteriaceae, Vibrio, Staphylococcus and Streptococcus. Genome analysis revealed enrichment in multiple biosynthetic gene clusters for nonribosomal peptides, polyketides, terpenes, and ribosomally synthesized and post-translationally modified peptides (RiPPs), supporting the biosynthetic potential of the strain. Functional annotations emphasize replication and repair modules, mobile element-associated proteins, helix–turn–helix regulators, and versatile transport systems, features coherent with cold stress and oligotrophic soils. Antibiotic susceptibility assays indicate a broad resistance phenotype under the experimental conditions tested, together with extracellular antimicrobial activity. These data refine the biogeography of A. fildesensis and indicate DEC002 as a credible Antarctic source of specialized metabolites with antimicrobial promise. Full article

Background: Tartary buckwheat (Fagopyrum tataricum) serves as an excellent model for studying plant water adaptation mechanisms due to its exceptional drought tolerance. While aquaporins (AQPs) mediate the transmembrane transport of water and solutes in plants, their fine-tuned regulatory networks underlying stress resilience in Tartary buckwheat remain largely elusive. Methods: Here, we combined bioinformatics and transcriptomics to systematically identify 30 highly conserved FtAQP genes at the genome-wide level. Results: Cross-validated by qRT-PCR, our analysis revealed their distinct expression patterns across different organs. Based on our transcriptomic data, we hypothesize that FtAQP family members potentially participate in a coordinated whole-plant water management network through differential spatiotemporal expression. Specifically, the robust transcription of FtAQP8, FtAQP12, and FtAQP28 in roots is associated with the initial water uptake process. As water undergoes long-distance transport, the synergistic upregulation of FtAQP13, FtAQP17, FtAQP20, and FtAQP29 in the stem suggests a potential role in facilitating critical lateral water flow. Furthermore, during reproductive development, FtAQP27 exhibits extreme tissue specificity in floral organs, implying its possible involvement in maintaining local osmotic homeostasis. Furthermore, the promoter regions of FtAQPs are highly enriched with cis-acting elements responsive to light, abscisic acid (ABA), and cold stress, suggesting they are intimately regulated by a coupling of endogenous phytohormones and environmental cues. Conclusions: Ultimately, this study provides valuable insights into the potential molecular basis of multidimensional water regulation in Tartary buckwheat, and identifies candidate genetic targets for improving water use efficiency in dryland agriculture through the precise manipulation of aquaporins. Collectively, while these observational findings provide valuable predictive models, future in vivo experimental validations are required to confirm their exact biological functions. Full article

The cultivation of Hericium erinaceus in China accounts for about 85% of the global supply. Its decentralized production systems have developed across diverse climate zones, leading to distinct, location-specific practices recorded in local technical standards. This scoping review synthesizes these empirical protocols from five agro-climatic regions. It illustrates how adaptive strategies such as cold-tolerant strains in the northeast and market-driven precision in the subtropics are associated with yield stability. These practices reflect two interconnected forms of diversity. One is the diversity of cultivation systems themselves, from forest-based methods to industrial-scale production systems. The other is the diversity of locally adapted strains developed for specific environments. We use the Intelligent Germplasm–Cultivation–Processing–Market (GCPM) Integration framework to connect local practices with broader questions of systemic resilience. The evidence draws on field-validated standards, not controlled experiments, reflecting the current state of research. This work presents China’s practical knowledge as a reference for designing context-sensitive, climate-resilient cultivation systems elsewhere, suggesting that resilience may depend more on intelligent adaptation to local conditions than on one-size-fits-all solutions. Full article

To explore the seasonal variation patterns of the skin microecology of Altay sheep under transhumant grazing conditions, skin swabs were collected from 60 free-grazing Altay sheep at seasonal transition nodes in the Altay region. Metagenomic sequencing combined with untargeted metabolomics was used to characterize their bacterial community structure, functional pathways, and metabolite profiles. The results showed that the skin microecology of Altay sheep presented obvious seasonal variation patterns. In spring, 35 of the 39 highly abundant bacteria were environmentally derived, five proliferation-related pathways were significantly enriched, and the levels of five metabolites associated with microbial community regulation and skin barrier defense were elevated. In summer, the abundance of three skin symbiotic bacteria increased, the activities of eight pathways mainly related to biofilm formation were significantly enhanced, and the contents of five metabolites primarily associated with membrane lipid homeostasis and selective bacteriostasis increased. In autumn, the abundances of nine radiation-resistant and cold-tolerant strains increased, together with the elevated abundance of two opportunistic pathogens; five repair-related pathways were active, and the levels of four anti-inflammatory and repair-associated metabolites were synchronously increased. In winter, the abundance of two cold-tolerant strains increased, the activities of pathways related to nitrogen metabolism and energy synthesis were enhanced, and one lignan compound was identified as the key metabolite. These findings elucidate the seasonal dynamic patterns of the skin microecology of Altay sheep and provide a theoretical basis for research on the adaptive mechanisms and seasonal health management of Altay sheep and other sheep in alpine regions. Full article

The ripe fruits of blackcurrant (Ribes nigrum L.) are rich in vitamin C, anthocyanins, and flavonoids. Besides being consumed fresh, the fruits can be processed into fruit juices, jams, wines, and other products, exhibiting considerable economic and nutritional value. Flavonoids are a class of important plant secondary metabolites with antioxidant, anti-inflammatory, and anti-cancer properties. Although previous studies have confirmed the involvement of multiple structural genes and transcription factors in flavonoid biosynthesis, the specific role of the dihydroflavonol 4-reductase (DFR) gene in regulating flavonoid accumulation during fruit development of blackcurrant remains to be clearly elucidated. In this study, we identified an RnDFR gene located in the nucleus and cytoplasm, which has the same expression trend as flavonoid content in fruit development stages. Overexpression of RnDFR improved the flavonoid accumulation and upregulated the expression levels of related structural genes (4CL, CHS, LDOX, ANR, and UFGT) in tomato. Transiently overexpressing RnDFR in blackcurrant fruit also increased the content of flavonoids and DFR enzyme activity, whereas silencing RnDFR resulted in the opposite effect. In addition, overexpression of RnDFR in tomato seedlings improved cold and drought tolerance by increasing flavonoid accumulation, reducing membrane lipid peroxidation damage and enhancing the activities of antioxidant enzymes. This study systematically reveals the key role of RnDFR in flavonoid biosynthesis and the enhancement of cold and drought tolerance, and offers an important theoretical basis for future efforts to optimize flavonoid content in blackcurrant and improve fruit nutritional quality. Full article

The sucrose synthase (SUS) gene family plays a pivotal role in plant carbon metabolism, growth, and development. In this study, we identified 65 SUS genes across six Brassica species (B. rapa, B. nigra, B. oleracea, B. juncea, B. napus, and B. carinata), and systematically analyzed their structural characteristics, evolutionary history, and expression profiles. Phylogenetic analysis classified these genes into three subfamilies (SUSI, SUSII, and SUSIII). SUS4 orthologs (from SUSI subfamily) are completely lost in Brassica, and total SUS gene numbers are just 6–7 in Brassica diploid species, though the SUSIII subfamily exhibits significant expansion in Brassica polyploid species. Selection pressure analysis (Ka/Ks) revealed that the Brassica SUS family has primarily undergone purifying selection, although certain members show evidence of adaptive evolution. Comprehensive expression profiling and qRT-PCR validation demonstrated the functional diversification of BnSUS genes in tissue specificity and responses to hormonal and abiotic stimuli. SUSI genes BnSUS1-1/2/3/4 are predominantly expressed in vegetative tissues and flowers; SUSII genes BnSUS2-1/2 and BnSUS3-1/2 are reproductive-organ-specific, while SUSIII genes BnSUS5-1/2 and BnSUS6-1/2/3/4 show young-plant-specific weak expression. BnSUS family genes are generally upregulated by ABA, TZ and GA but downregulated by IAA, ACC, BL and JA. Salt, drought, freezing and cold mainly upregulate the BnSUS family, heat downregulates it, and osmotic stress exerts both effects. Correspondingly, Brassica SUS promoters are enriched with light-responsive (G-box, Box-4), hormone-responsive (ABRE, CGTCA-motif) and anaerobic-induction (ARE) elements. Functional characterization demonstrated that the ABA-responsive gene BnSUS3-2 significantly improved tolerance to osmotic and ionic stresses by promoting root growth in transgenic A. thaliana seedlings. These findings underscore the essential roles of BnSUS genes in maintaining cellular homeostasis and provide a theoretical foundation for the genetic improvement of carbon metabolism and stress resilience in Brassica crops. Full article

Shoot tip cryopreservation is essential for the long-term conservation of plant genetic resources. It provides the only reliable method for establishing a long-term, readily available gene pool of clonally propagated crops and elite in vitro clones used in the pharmaceutical, food, and cosmetic industries. Still, its success is often limited by the inherent sensitivity of many species to the osmotic and chemical stresses imposed by concentrated cryoprotectant (vitrification) solutions and severe dehydration. The optimization of modern cryopreservation protocols primarily focuses on modifying shoot tip preculture, cryoprotectant treatments, or regrowth conditions, while frequently overlooking donor plant preconditioning or relegating it to a secondary role. However, the physiological state of in vitro plants from which apical or axillary shoot tips are extracted may hold the key to successful post-cryopreservation recovery, especially in cryo-sensitive taxa. This review revisits the critical role of donor plant vigor and induced stress tolerance in the cryopreservation of clonal crops by systematically evaluating preconditioning strategies, including cold acclimation, sucrose pretreatment, and the use of growth regulators and signaling molecules such as abscisic, jasmonic, and salicylic acids, involved in stress signaling and tolerance development. The beneficial physiological changes induced by donor plant pretreatment, such as reduced freezable water content and the accumulation of protective compounds, are discussed in the context of contemporary cryopreservation methods. The effects of culture conditions, including the roles of ammonium and nitrates, light quality, culture density and aeration, medium strength, culture age, and subculture duration, are also considered. We analyze how different treatments of in vitro donor plants improve shoot tip tolerance to osmotic and/or chemical toxicity imposed by specific cryopreservation methods to support a material-centered selection of a cryopreservation procedure. Future directions and potential approaches for integrating target donor plant preconditioning into modern cryopreservation protocols for shoot tips, particularly in stress-sensitive species, are discussed. Full article

This study investigated the adaptive mechanisms of silver pomfret in response to chronic low-temperature stress, focusing on the tissue-specific expression patterns of the key lipid metabolism gene scd1 and its central role in regulating hepatic apoptosis. A gradual cooling experiment (from 18 °C to 6 °C) was conducted to analyze the spatiotemporal expression profiles of ten lipid metabolism-related genes across six tissues. The results revealed that the most pronounced changes were observed in the heart, liver, and gills. The liver and heart rapidly activated genes involved in lipid breakdown and utilization from 16 to 12 °C for immediate energy supply, while gill tissue upregulated the pi3k/p450/srebp/scd1 pathway at 10 °C to remodel membrane lipids against sustained stress. Further in vitro hepatocyte experiments demonstrated that scd1 expression directly regulated cell survival and apoptosis under low temperatures. Knockdown of scd1 significantly promoted apoptosis, whereas its overexpression effectively suppressed it. Moreover, scd1 expression was intricately modulated by its upstream regulators srebp (positive regulation) and pparγ (showing potential negative feedback at specific temperatures). This study systematically elucidates the pivotal role of scd1-mediated lipid metabolic reprogramming in the cold adaptation of silver pomfret, providing a crucial theoretical foundation for deciphering the molecular mechanisms of cold tolerance and for breeding cold-resistant strains. Full article