Search Results (2,615)

The Transformer project at TU Wien exemplifies how universities can act as initiators for sustainability education and STEM science communication. Addressing the urgent need for climate change adaptation and the strengthening of key competencies for sustainability, this paper examines the university’s role in creating a temporary, participatory learning space. The Transformer bridges academic research and societal practice, demonstrating how higher education institutions can actively shape sustainable development. As both a knowledge producer and a facilitator of public dialogue, TU Wien designs the Transformer as a hands-on laboratory for children and adolescents, providing low-threshold access to STEM and sustainability content. By integrating university students and researchers from architecture, civil and environmental engineering, mechanical engineering, electrical engineering, informatics, and technical chemistry, the project transforms abstract scientific concepts into tangible, co-created solutions. This approach is designed to foster systemic thinking, practical skills, and participant agency—outcomes for which this paper presents documented but preliminary evidence—while enriching academic teaching and research through real-world applications. This paper analyses how TU Wien’s commitment to interdisciplinary cooperation and participatory science communication positions the Transformer as a model for other institutions, with a focus on circular economy as the anchor theme. Through reflective practice on four analytical dimensions—place-based learning, staged sustainability education, co-creative STEM communication, and the engaged university—this study offers insights into the challenges and opportunities of university-led co-creation spaces and considerations for institutions seeking to develop comparable initiatives. Full article

Intestinal barrier dysfunction and mucus layer abnormalities are central features of inflammatory bowel disease, yet the epithelial mechanisms regulating goblet cell function remain incompletely understood. The TWEAK/Fn14 pathway is involved in intestinal inflammation, but its role in small intestinal epithelial responses, particularly in goblet cells, has not been fully clarified. In this study, we investigated TWEAK/Fn14 signaling using mouse small intestinal epithelial organoids. Fn14 was expressed in MUC2-positive cells in intestinal tissue, with staining preferentially observed toward the mucin-facing region. Fn14 staining was also observed in small intestinal epithelial organoids. TWEAK stimulation increased Tnf mRNA expression by approximately 1.56-fold and reduced Muc2 mRNA expression to approximately 66% of control, without markedly affecting epithelial proliferation or stem cell markers. TWEAK did not significantly alter the PAS-positive mucin area or AB-PAS-defined mucin composition, whereas PGM34 staining showed a non-significant tendency to increase. TNF treatment reproduced some of the TWEAK-associated changes, including reduced Muc2 mRNA expression and alterations in mucin-associated parameters, suggesting that TNF may contribute to some of the epithelial responses associated with TWEAK treatment. Treatment with an anti-TNF neutralizing antibody modified several TWEAK-associated responses, including changes in epithelial-associated gene expression and mucin-associated staining parameters. These findings suggest that TWEAK treatment is associated with goblet cell-related mucin responses in mouse small intestinal epithelial organoids, with possible involvement of TNF signaling. Full article

Cadmium contamination in water poses a serious environmental and health concern due to its high toxicity and persistence. In this context, the development of efficient and low-cost adsorbent materials has attracted increasing attention. This work examines the removal of Cd(II) from aqueous solution using a cellulose acetate–clay composite membrane as an adsorbent material. To evaluate the impact of clay inclusion, membranes were fabricated with varying clay concentrations (0%, 12.5%, and 25%). A Box–Behnken design was used to optimize the process; thermogravimetric analysis, X-ray diffraction, and Fourier-transform infrared spectroscopy were used to analyze the produced composite membranes. The characterization results confirmed the successful incorporation of clay into the cellulose acetate matrix and revealed important changes in the membrane structure and surface morphology. The adsorption performance was strongly affected by operating conditions, particularly temperature, contact time, and clay content. Under the optimal conditions of 30 °C, 4 h, and 5% clay content, the CA-Clay composite membrane achieved a maximum Cd(II) removal efficiency of 93.93% and an adsorption capacity of 12.35 mg/g. These results demonstrate that the composite membrane has a high affinity toward Cd(II) ions, exhibiting its high potential as a low-cost and efficient adsorbent for the removal of Cd(II) from aqueous solution. Full article

Background: Unexplained cytopenia represents a common diagnostic challenge in clinical haematology, reflecting either benign conditions or early manifestations of myeloid neoplasms. Methods: In this retrospective cross-sectional study, we analysed 557 patients with cytopenia who underwent initial diagnostic evaluation. The diagnostic performance of the initial morphological assessment of the bone marrow and ancillary studies excluding genetic analyses was evaluated against a genetically supported reference diagnosis established by a multidisciplinary haematology tumour board. We examined the extent to which the initial morphological assessment could reliably detect or rule out myeloid neoplasia. The analysis was performed for the entire cohort and stratified by specific myeloid neoplasm entities. Results: In the overall cohort, the initial morphological assessment of the bone marrow showed high diagnostic performance (sensitivity, 0.864; specificity, 0.934). The misclassification analysis revealed more false negatives than false positives (31 vs. 21), with no significant asymmetry. In the entity-specific analysis, a sensitivity of 1.0 was observed for acute myeloid leukaemia, with a reduced specificity (0.400). Myelodysplastic neoplasms exhibited a higher rate of false-negative findings, whereas myelodysplastic/myeloproliferative overlap neoplasms were characterised by very high sensitivity (0.982) and limited specificity (0.632). A comparable diagnostic pattern was observed for myelofibrotic neoplasms. Conclusions: Initial morphological assessment demonstrates high overall diagnostic performance in cytopenia but shows important variation across individual entities. These findings highlight the need to consider entity-specific limitations and the role of genetic confirmation in the diagnostic work-up of specific entities. Full article

The growing demand for sustainable, non-animal-derived, and low-allergenic protein alternatives has driven research into innovative sources such as microalgae. This review focuses on three key microalgal species Arthrospira platensis (Spirulina), Chlorella vulgaris, and Tetraselmis chuii. It examines their cell wall structures, protein content, and amino acid profiles. Protein extraction methods, including physical, enzymatic, and chemical approaches, are critically discussed. Downstream purification techniques aimed at improving protein purity and quality are also reviewed. Protein characterization methods are discussed, highlighting their relevance to food applications. The potential applications of microalgal biomass and protein extracts in food and beverage products are evaluated, with consideration given to their functionality, safety, and regulatory aspects. Despite significant advances in this field, further research is essential to optimize extraction and processing technologies, facilitate their integration into mainstream food production, and improve overall process efficiency. Full article

Chemistry teaching in youth and adult education has faced challenges related to students’ disengagement, interrupted educational trajectories, and difficulty in connecting scientific concepts with learners’ everyday and professional experiences. In Brazil, Professional and Technological Secondary Education Integrated with Youth and Adult Education (PROEJA) aims to address its challenges by combining basic education with vocational training. This study investigated how a contextualized pedagogical intervention based on the production of aromatic candles contributed to students’ engagement, contextualized conceptual learning and vocational reflection in a technical administration course offered at the Brazilian Federal Institute (IFMS-CG), Campo Grande, Mato Grosso do Sul, Brazil. A qualitative participatory action research design was adopted, and qualitative data were generated through participatory observations, classroom interactions, students’ written reflections, photographic records, and reflective discussions. Data were interpreted by Reflexive Thematic Analysis. Throughout the intervention, students explored Organic Chemistry concepts related to hydrocarbons, n-paraffins, physicochemical properties, volatility, combustion, formulation and laboratory safety while simultaneously discussing entrepreneurship, branding, product development and income generation. The qualitative findings demonstrated students’ active participation, collaborative teacher–student interactions, contextualized conceptual learning and a broader perception of Chemistry as a socially and professionally relevant discipline connected to everyday life, vocational education and entrepreneurial practice. Students’ written reflections further revealed that scientific knowledge was increasingly associated with technological innovation, product quality, professional opportunities and income generation. These findings suggest that contextualized, interdisciplinary and humanized pedagogical practices may promote meaningful scientific learning while strengthening vocational reflection and reinforcing the relevance of Chemistry within youth and adult professional and technological education. Full article

Electrochemical impedance spectroscopy (EIS) resolves the internal physicochemical processes of lithium-ion batteries across timescales—from ohmic conduction through charge-transfer kinetics to solid-state diffusion. Despite this analytical power, EIS deployment remains largely confined to laboratory electrochemical workstations that are bulky, expensive, and incapable of online multi-cell operation under dynamic DC bias conditions. This study presents a multi-channel EIS measurement device that simultaneously addresses three requirements for practical battery diagnostics: workstation-grade measurement accuracy, multi-cell synchronous acquisition, and tolerance to the DC bias voltage present across battery terminals during operation. The device employs a master–slave distributed architecture: each slave unit is built around the DNB1101 battery-dedicated impedance measurement chip with a Kelvin four-wire sensing configuration, while the STM32F407-based master controller coordinates measurement scheduling and data communication under FreeRTOS. A four-channel slave board with a differential daisy-chain communication topology and hardware broadcast trigger mechanism supports multi-cell synchronous acquisition. The device operates over a frequency range of 0.01 Hz to 5620 Hz with logarithmic spacing, and a C#-based host application provides real-time Nyquist and Bode visualization along with MATLAB R2024a-based post-processing for outlier rejection and data smoothing. Validation was conducted using Panasonic NCR18650 ternary (NCA) and LiFePO₄ (LFP) 18650 cells, benchmarked against a CorrTest CS350 electrochemical workstation at SOC = 40% and 25 °C. The device achieves a maximum impedance magnitude error of 1.55% and a maximum phase error of 1.22%. Equivalent circuit model fitting via ZSimpWin yields parameter differences below 1% between the device and the reference workstation. Under online conditions with a 3.6 V DC bias, the impedance measurement deviation of a 20 mΩ precision resistor remains below 0.69% across the full frequency range. Multi-channel synchronous measurements across four cells demonstrate inter-channel amplitude variance below 2.13%. Cross-chemistry validation with LiFePO₄ cells yields magnitude and phase errors below 0.92%. These results demonstrate that the proposed device provides laboratory-grade EIS accuracy with multi-channel, online, and cross-chemistry capabilities, offering a practical platform for integrating EIS-based diagnostics into next-generation battery management systems. Full article

Human immunodeficiency virus (HIV) is an enveloped virus with a remarkable capacity for genetic diversification, enabling rapid escape from host immune responses and therapeutic interventions. Despite extensive global efforts, the development of an effective vaccine has remained elusive owing to the virus’s high genetic variability and antigenic diversity. Consequently, considerable effort has been directed toward the development of therapeutic agents targeting viral entry, reverse transcriptase, integrase, protease, and more recently, capsid. Although antiretroviral therapy (ART) remains the cornerstone of HIV treatment, it is associated with challenges including drug resistance, adverse side effects, and limitations in access and affordability. Targeting viral entry offers distinct advantages by blocking infection at the earliest stage of the viral life cycle and enabling the neutralization of free virions, as well as Fc-mediated elimination of HIV-infected cells in some cases. This review highlights promising protein-based HIV entry inhibitors that have demonstrated efficacy in preclinical studies, and discusses ongoing efforts to optimize their valency, avidity, specificity, serum half-life, effector functions, and production platforms to improve their therapeutic potential and economic feasibility. Full article

Background/Objectives: Pharmacokinetic and pharmacodynamic drug–drug interactions are major determinants of drug safety in polypharmacy, with potential consequences including reduced therapeutic efficacy, altered drug exposure, and increased adverse effects. This study presents DDI-HierPred, an artificial intelligence-based hierarchical framework for drug–drug interaction prediction using Morgan fingerprint-based drug-pair representations. Methods: At Level 1, Logistic Regression, Random Forest, Linear SVM, and XGBoost were compared for pharmacokinetic/pharmacodynamic (PK/PD) classification. At Level 2, an XGBoost multiclass classifier was used to predict 61 interaction subtype classes. End-to-end performance was evaluated using predicted Level 1 routing, and additional drug-identity-disjoint evaluations were conducted to assess generalization when one or both drugs were unseen during training. Y-randomization analyses were performed for both classification levels. Results: Linear SVM achieved the best Level 1 performance, yielding an accuracy of 0.8661, a ROC-AUC of 0.9380, and an MCC of 0.7303 on the independent test set. At Level 2, the XGBoost classifier achieved an accuracy of 0.8712, a balanced accuracy of 0.9087, a macro F1-score of 0.9115, and a Top-3 accuracy of 0.9868. Because the Level 2 test partition was also used for algorithm comparison and model selection, these results should be regarded as exploratory and potentially optimistic rather than as an independent final evaluation. When evaluated end-to-end using predicted Level 1 routing, performance decreased to an accuracy of 0.5767, balanced accuracy of 0.3573, and macro F1-score of 0.4379. Additional drug-identity-disjoint evaluations showed further performance reductions when one or both drugs were unseen during training, highlighting the greater difficulty of generalization to previously unseen drug identities. Y-randomization analyses supported the robustness of both classification levels. Conclusions: The framework was deployed as a publicly accessible web platform integrating documented interaction lookup, hierarchical PK/PD classification, Level 1-constrained subtype prediction, confidence scoring, single-pair and batch analysis, ranked Top-3 predictions, and downloadable reports. These findings indicate that upstream routing and unseen-drug generalization remain important limitations of the current framework. DDI-HierPred therefore provides a computational platform for research-oriented screening, interpretation, and prioritization of potential drug–drug interactions. Full article

Local physicochemical variability in small post-extraction gravel pit lakes is often difficult to interpret because water chemistry may reflect both seasonal dynamics and highly local shoreline conditions. This study assessed spatial and temporal patterns in selected physicochemical parameters of near-surface water at eight fixed littoral sampling points representing contrasting riparian and land-use settings in two gravel pit lakes within the Międzyrzeckie Jeziorka lake complex near Międzyrzec Podlaski, eastern Poland. Surface-water monitoring was conducted from 9 November 2024 to 23 September 2025. The analytical dataset covered total organic carbon, inorganic carbon, total carbon, total nitrogen and laboratory-measured pH. The strongest and most consistent changes occurred between sampling dates in both lakes. Differences among fixed points were limited; total nitrogen differed between two points in Gravel pit lake no. 1, while the other parameters showed no date-adjusted point-level pattern over the monitored period. Mean total organic carbon and total carbon concentrations were similar in the two lakes, whereas Gravel pit lake no. 1 showed higher maximum values for most parameters and greater variability in selected carbon and nitrogen fractions. Laboratory-measured pH was generally weakly acidic to near-neutral, and the lowest observations occurred during individual campaigns. The findings show pronounced temporal dynamics and a restricted spatial signal in the monitored littoral zones. Repeated fixed-point sampling provided a clear basis for comparing temporal changes and local differences in these post-extraction lakes. These inferences are restricted to the monitored near-surface physicochemical variables. Full article

Curcumin derivatives (curcuminoids) exhibit considerable antiparasitic and antiviral activities. In this study, EF24-like bis-2-fluorobenzylidene piperidone derivatives were synthesized and evaluated for antiprotozoal activity and inhibition of the human immunodeficiency virus (HIV)-1 reverse transcriptase (RT)-associated RNase H (HIV-1 RNase H). A series of bis-arylidene piperidones and tetrahydro(thio)pyranones bearing halogen or nitro substituents were tested against Leishmania major (promastigotes and amastigotes) and Toxoplasma gondii. L. major promastigotes were the most sensitive parasitic model, with several compounds exhibiting low-nanomolar IC₅₀ values, while ortho- or para-halogenated analogues demonstrated submicromolar activity against T. gondii. Among them, 3,4-dichlorophenyl (HPip-DC) and 4-bromophenyl (HPip-4Br) derivatives showed potent activity against L. major and HIV-1 RNase H. The entropy-uncorrected MM-GBSA effective binding energy estimates were −33.41, −9.29, −8.37, and −4.27 kcal/mol for the predicted L. major squalene monooxygenase–HPip-DC, bovine cytochrome bc₁–DiFiD (2,4-difluorophenyl derivative), RNase H–HPip-4NO (4-nitrophenyl derivative), and RNase H–HPip-DC complexes, respectively. During 300 ns simulations, the ligands remained associated with their binding pockets. The Arg557 residue was crucial for HPip-4NO-mediated RNase H inhibition while the inhibitory activity of HPip-DC was much less dependent on this amino acid. These findings identify EF24-like curcuminoids with potent in vitro antiprotozoal activity and biochemical inhibition of HIV-1 RNase H. Antiviral activity was observed but confounded by host cell toxicity. Full article

Kaempferol, a naturally occurring dietary flavonoid abundantly found in various fruits and vegetables, has garnered significant attention due to its diverse pharmacological properties, most notably its potent anticancer activity. In the orchestration of cancer pathogenesis, the PTEN/PI3K/AKT/mTOR signaling cascade plays a pivotal role, where aberrant activation or dysregulation of this pathway drastically accelerates tumor cell proliferation, survival, and metabolic reprogramming, thereby driving tumorigenesis. Moreover, non-coding microRNAs (miRNAs) have emerged as key modulators involved in regulating this pathway, functioning as either oncogenes or tumor suppressors to determine cancer cell fate. Previous studies have demonstrated that kaempferol and its derivatives, owing to their molecular structures, suppress the PI3K/AKT/mTOR signaling pathway either directly or indirectly, thereby playing a critical role in inhibiting cancer cell proliferation. Hence, this comprehensive review elucidates the therapeutic potential of kaempferol and its derivatives, focusing specifically on their molecular mechanisms in modulating the PTEN/PI3K/AKT/mTOR pathway. Furthermore, this review highlights the crosstalk between kaempferol and the specific miRNAs targeting these signaling components in various cancer types. Full article

The 1st International Online Conference on Environments addresses issues of environmental understanding, management, and restoration. Sessions ranged from general frameworks to a broad area of specific investigations: heavy metal removal using inactive yeast, gadolinium’s aquatic toxicity, adsorptive removal of pollutants, energy recovery from waste and wastewater treatment, recycled carbon fibers, climate-resilient urban development, renewable biofuel production, green hydrogen, anthropogenic and volcanic CO₂ emissions, quantifying aging dignity in urban ecosystems and stray dogs as pollution health sentinels. Keynotes covered digital plant phenotyping for restoration, microplastic dynamics, agricultural residue management, carbon credits, air quality, atmospheric pollution, transitional waters, green chemistry, ecotoxicity, micropollutants, and coastal darkening effects on plankton, among others. Applied solutions included phytoremediation of eutrophication in urban streams, circular approaches in aquaculture, biochar for wastewater treatment, AI-assisted mangrove monitoring, and true-cost accounting for food systems. The conference demonstrated that effective environmental policy requires the integration of laboratory findings, field restoration, and shared resource responsibility across terrestrial and marine ecosystems. Full article

Background/Objectives: Kidney graft dysfunction remains a major challenge limiting long-term transplant survival despite advances in immunosuppressive therapy. Cytokine signaling pathways have been implicated in the regulation of alloimmune responses and chronic inflammation, but their contribution to long-term graft function and immunosuppressant pharmacokinetics remains incompletely understood. This study investigated associations among selected cytokine gene polymorphisms (IL-2 −330T>G, IL-6 −174G>C, and IL-10 −1082A>G), biochemical and hematological parameters, and calcineurin inhibitor (tacrolimus and cyclosporine) dosing in kidney transplant recipients. Methods: A total of 394 kidney transplant recipients receiving tacrolimus or cyclosporine were enrolled. Genotyping of IL-2 rs2069762, IL-6 rs1800795 and IL-10 rs1800896 was performed using real-time PCR. Clinical, biochemical, and pharmacokinetic data were analyzed using univariate and multivariate statistical models adjusted for relevant demographic and clinical covariates. Results: IL-2 rs2069762 and IL-6 rs1800795 polymorphisms were not associated with clinically relevant differences in biochemical parameters, calcineurin inhibitor dosing, or transplant outcomes. For IL-10 rs1800896, no statistically significant associations were observed with cyclosporine A blood levels, dose, or C/D ratio. Among tacrolimus-treated patients, the regression model for the C/D ratio was borderline significant (F(2,207) = 3.048, p = 0.050, R² = 0.029). However, the genotype-specific association for the heterozygous GA genotype versus AA was not statistically significant in the unadjusted model (B = −0.462, 95% CI: −0.97 to 0.04, p = 0.072) or after adjustment for age, sex, and time since transplantation (B = −0.450, 95% CI: −0.95 to 0.05, p = 0.078). Although univariate analyses identified associations between IL-10 rs1800896 and selected biochemical parameters, these findings were not confirmed in multivariable models. No significant associations were observed between the analyzed cytokine polymorphisms and graft rejection, graft survival, patient survival, or clinically relevant markers of kidney graft function. Conclusions: The results suggest a possible, although not statistically significant, association between IL-10 rs1800896 and tacrolimus exposure, as assessed by the C/D ratio. This observation should be considered preliminary and interpreted with caution. The lack of CYP3A5 genotyping and functional assessment of IL-10 activity, inflammation, and CYP3A-dependent metabolism further limits the interpretation of this potential association. Larger prospective studies with comprehensive pharmacogenetic and functional characterization are needed to determine whether IL-10 rs1800896 contributes to interindividual variability in tacrolimus exposure and whether it may have potential applications in individualized immunosuppressive therapy. Full article

Aronia melanocarpa contains abundant phenolic compounds, including procyanidins, flavonols, flavan-3-ols, and phenolic acids, but its pronounced astringency limits its broader use in food applications. Catechol and other oxygen-donor motifs in plant phenolics are known to associate with Fe³⁺ and can support metal–phenolic assembly. In this study, ultrasound-assisted cellulase–pectinase extraction was used to recover an A. melanocarpa polyphenol extract, which was subsequently combined with Fe³⁺ to construct a plant-derived hair dye coating on keratin fibers. Under optimized extraction conditions, the total phenolic yield reached 82.315 mg gallic acid equivalents (GAE)/g dry fruit. A limited targeted LC–MS/MS panel was used to evaluate 12 selected non-anthocyanin phenolic analytes, including procyanidin dimers, a flavan-3-ol, flavonols, and phenolic acids; the contribution of the pH-sensitive anthocyanin fraction was not resolved. Among the tested metal ions, Fe³⁺ produced the strongest chromogenic response. The optimized L-cysteine pretreatment–Fe³⁺ mordanting–polyphenol dyeing sequence produced a ΔE* of 55.42. Adsorption experiments empirically described the uptake behavior of the polyphenol extract, whereas complementary surface, spectroscopic, elemental, diffraction, thermal, and wettability analyses were consistent with the presence of a relatively uniform Fe-containing polyphenol coating at the hair fiber interface. Neither the adsorption-model fits nor the individual characterization techniques uniquely resolved the underlying molecular mechanism. Preliminary HET-CAM, single-exposure dermal, and acute eye irritation assessments showed no obvious acute irritation under the tested conditions. An exploratory image-based analysis examined whether standardized hair tress photographs could approximate a predefined colorimetric score. Because only 30 independent original samples were available and no external validation was performed, this analysis was treated solely as a proof of concept. This laboratory proof-of-concept extends established Fe³⁺–polyphenol assembly chemistry to a compositionally complex A. melanocarpa extract for hair fiber coloration. Further work is required to clarify anthocyanin behavior, simplify the sequential protocol, and benchmark its performance against representative commercial hair dyes. Full article