Search Results (34,070)

Allergic conditions have surged to unprecedented levels globally, affecting approximately 30% of the global population. Fungi are among the most significant sources of allergens, accounting for approximately 6% of respiratory issues in the general population. However, identifying the precise cause of respiratory allergies remains challenging. We investigated the potential of two rust species, Tranzschelia pruni-spinosae and Phragmidium rubi-idaei, which infect common fruit plants, to induce inflammatory and asthmatic responses in mouse models of both acute and chronic asthma. Mice were sensitized and administered intranasal challenges with extracts from T. pruni-spinosae and P. rubi-idaei. Levels of pro-inflammatory cytokines (IL-4, IL-5, IL-13, TNF-α, and TGF-β) were measured via ELISA. Additionally, specific IgE production was assessed via ELISA and lung histology was examined using hematoxylin-eosin staining. Both fungal extracts induced significant increases in all tested cytokines, elevated specific IgE levels, and histological changes characteristic of acute and chronic asthma progression in the lungs. The microfungi T. pruni-spinosae and P. rubi-idaei possess strong proinflammatory and asthma-inducing capabilities, suggesting their potential as previously unrecognized fungal allergens. Full article

To address the challenges of strong electromechanical coupling, nonlinear friction, and poor disturbance rejection in semi-direct-drive scraper conveyor systems under complex coal mining conditions, this paper aims to propose a high-performance drive control strategy that balances dynamic response speed with steady-state operational smoothness. First, an integrated electromechanical coupling dynamic model incorporating Permanent Magnet Synchronous Motor (PMSM) vector control and the time-varying meshing stiffness of a two-stage planetary gear train is established. Subsequently, a Sliding Mode Control (SMC) strategy optimized with a saturation boundary layer is designed and compared with traditional Proportional-Integral (PI) control under multiple operating conditions. Time-frequency domain analysis indicates that SMC significantly enhances the dynamic stiffness of the drive system. Under sudden load change conditions, the speed recovery time is shortened by approximately 76%, and the steady-state error is reduced by 37% compared to PI control. Microscopic characteristic evaluation based on FFT and Total Variation (TV) metrics reveals that SMC achieves active disturbance rejection through spectral broadening of the electromagnetic torque. Crucially, the steady-state cumulative control effort of SMC is equivalent to that of PI, implying no additional mechanical stress burden, while the equivalent dynamic transmission force fluctuation in the mechanical chain is reduced by about 3%. The study confirms that the proposed strategy successfully achieves a synergistic optimization of “macroscopic rapid response” and “microscopic smooth operation,” providing a theoretical basis for the high-precision control of heavy-duty underground transmission equipment. Full article

To address the energy dissipation requirements of hydraulic engineering projects with medium-low water heads and medium-high unit discharges, counterflow-type underflow energy dissipation can significantly enhance the energy dissipation efficiency through the head-on collision of flows from spillways on both sides. In this study, the spillway of the Lieshen Reservoir was used as the prototype. Since gravity dominates the flow in spillways, we established a 1:15 physical model based on the Froude similarity criterion, and conducted numerical simulations using the volume of fluid method coupled with the realizable k-ε turbulence model. Furthermore, the hydraulic characteristics of counterflow energy dissipation under different flow rates and stilling basin length conditions were analyzed. The results show that the counterflow energy dissipation rate first increases before decreasing with increasing stilling basin length, and the maximum energy dissipation rate can exceed 85%; however, the change in the stilling basin depth has a small impact on the energy dissipation rate, especially under relatively high flow rates; furthermore, an empirical formula for the conjugate depth after a hydraulic jump suitable for counterflow energy dissipation with Froude number in the range of 2.0 < Fr₁ < 9.7 and stilling basin depth of 0.5–1.5 m is proposed, with the relative error between its predicted and simulated values being less than 6%. Based on the analysis of the water depth outer envelope curve at the outlet section of the stilling basin, it is suggested that the sidewall height be set to 0.6–0.8 times the conjugate depth after the hydraulic jump. Full article

Engineered wood is increasingly valued for its use of renewable raw materials, low environmental impact, and good performance characteristics. The wide variety of raw materials used to produce engineered wood requires detailed research to select rational technological production parameters. This paper examines engineered wood produced from furniture-recycling waste and different polyurethane compositions. The factors under analysis include mixture pressure level, binder type, and filler preparation. The effect of the mixture’s pressure level on density, structure, strength, and moisture indicators was evaluated. The pressure levels were 1.5, 2.0, 2.5, 3.0, and 3.5 MPa. Tests have shown that the mixture pressure level significantly affects compressive strength, absorption, swelling, and structure formation. A rational pressure level of 3.0 MPa was determined. When using this level, there are no voids in the samples. Depending on the binder composition, the resulting compressive strength ranges from 20.5 to 25.6 MPa, water absorption from 7.62 to 10.3 kg/m², and swelling from 8.65 to 12.6%. At a pressure of 3.5 MPa, the properties of engineered wood improve further, but the binder begins to separate from the mixture. A significant influence of the binder on compressive strength, absorption, and swelling was observed. A detailed kinetic analysis was performed to evaluate changes in sample strength following production. The tests showed that the compressive strength increased markedly 14 days after sample preparation. Full article

Objective: The primary objective of this study is to investigate the prevalence of unexpected findings requiring treatment after routine histological examinations following tonsillectomy, tonsillotomy, adenotomy, or conchotomy (TTAC) in a retrospective study and to discuss whether routine histological examination is useful in patients without clearly defined risk factors or whether it would be better to reduce unnecessary costs and resource utilisation. Materials and methods: The present retrospective study encompasses 5709 patients who underwent routine histological examinations following TTAC and were treated as inpatients at the University Medical Center Freiburg, Department of Otolaryngology, Head and Neck Surgery, between 2011 and 2021. The data was collected based on patient characteristics, including date of birth, gender, age of patients at the time of surgery, date of surgery, indication for surgery, tissue examined, and histological result. Results: Of a total of 6687 patients who underwent TTAC, 5709 with routine histological examinations were included in the analysis, of whom only four showed abnormal findings, corresponding to an overall prevalence of 0.07%. Three of these four patients were adults. These included two cases of granulomatous inflammation, one instance of Burkitt lymphoma, and one instance of chronic lymphocytic leukaemia/small cell B-lymphoma. Following the exclusion of tuberculosis and sarcoidosis, and the lymphoma board’s decision to adopt a watch-and-wait approach in the case of chronic lymphocytic leukaemia/small cell B-cell lymphoma, only n = 1/0.0175% of patients were found to require treatment. Conclusions: The study demonstrated that only four abnormal histological findings occurred in 5709 inpatient TTACs, of which only one, namely Burkitt lymphoma, ultimately required treatment. Consequently, it can be concluded that routine histological examinations following TTAC are not beneficial in patients without clearly defined risk factors, such as blood in the saliva, history of smoking or alcohol consumption, unexplained pain, previous cancer, mucosal changes, or tissue asymmetries. However, in instances where clinical or anamnestic suspicion of malignancy is present, a histological examination should be conducted. Full article

Background: Gum Arabic (GA) is a natural polysaccharide widely recognized for its antioxidant and anti-inflammatory properties; however, its functional behavior as a biopolymeric gel and the mechanisms underlying its selective effects on cancer-related redox microenvironments remain insufficiently characterized. It is imperative to note that the interaction between its physicochemical properties and its biological activity in colorectal cancer remains to be fully clarified. Methods: This study aimed to evaluate the antineoplastic potential of GA in human colorectal cancer (CRC) cell lines (HT-29 and HCT-116) compared to normal fibroblasts (MRC-5) using the MTS assay. Oxidative stress-related molecular responses were assessed by quantitative PCR analysis of GPX4, GSTA2, CAT, NFKB, and SOD1 expression. In parallel, extracellular concentrations of key metal ions (Fe²⁺, Zn²⁺, Mn²⁺, Mg²⁺, Cu²⁺, and Al³⁺) were quantified following GA exposure. To establish its functional gel characteristics, rheological measurements were performed to assess viscosity and shear-dependent behavior, and USP-compliant in vitro kinetic studies were conducted to evaluate time-dependent release properties. Results: GA induced dose-dependent cytotoxicity in HT-29 and HCT-116 colorectal cancer cells, while MRC-5 fibroblasts exhibited comparatively higher viability across the tested concentration range, indicating reduced sensitivity in normal cells. Rheological analysis revealed concentration- and ion-dependent viscoelastic behavior, identifying a 10% (w/w) GA formulation as optimal due to its balanced low-shear viscosity and controlled shear-thinning properties. Kinetic studies demonstrated a defined, diffusion-governed release profile under physiologically relevant conditions. At the molecular level, significant upregulation of GPX4 and GSTA2 was observed in both cancer cell lines, whereas NFKB expression increased selectively in HT-29 cells, with no notable changes in CAT or SOD1 expression. Additionally, GA treatment resulted in marked increases in Fe²⁺, Zn²⁺, and Mn²⁺ levels, indicating modulation of the redox–ionic microenvironment. Conclusions: These findings demonstrate that GA functions as a natural, ion-responsive biopolymeric system with defined rheological and kinetic properties, capable of selectively targeting colorectal cancer cells through coordinated genetic and ionic regulation of oxidative stress. Collectively, the results position GA as a promising functional gel-based platform for future redox-modulated therapeutic strategies in colorectal cancer. Full article

Background: Point-of-care ultrasound (POCUS) has emerged as a valuable tool for rapid diagnosis, procedural guidance, and real-time clinical decision-making in neonatal and pediatric critical care. Despite its growing use in acute medicine, the evidence describing its implementation, utility, and impact in interfacility and prehospital transport settings remains limited. This scoping review aims to systematically map the current body of evidence on POCUS use during neonatal and pediatric transport and to identify knowledge gaps to inform future research, training, and clinical integration. Methods: A scoping review was conducted following PRISMA-ScR 2020 guidelines, searching PubMed, Embase, Scopus, CINAHL, and Web of Science for studies describing POCUS use during neonatal and pediatric transport. Results: Of 3676 unique articles identified, 20 met inclusion criteria, including 10 cohort studies, 3 case series, 4 case reports, 2 narrative reviews, and 1 textbook chapter. Fifteen studies reported extractable patient-level data and were included in quantitative synthesis, encompassing 4278 patients. Among these, 1153 (27.0%) patients were under 18 years old, and 576 (13.5%) had POCUS performed during transport. POCUS was primarily used for diagnostic assessment—mainly lung and cardiac imaging—with variability in protocols, operator training, and transport characteristics. Eleven studies (73.3%) reported that POCUS altered clinical management, influencing management in 106 (18.4%) patients through diagnostic clarification, resuscitation decisions, medical or ventilator adjustments, and changes in transport destination. Conclusions: Evidence suggests that POCUS supports clinical decision-making and timely intervention during neonatal and pediatric transport, though use remains inconsistent. Future studies should focus on developing structured training frameworks, validating transport-specific protocols, and assessing the impact of POCUS on clinical outcomes and transport safety. Full article

Developmental dyslexia (DD) often involves difficulties in phonological processing of speech. Objectives: While underlying neural changes have been identified in terms of stimulus- and task-related responses within specific brain regions and their neural connectivity, there is still limited understanding of how these changes affect the overall organization of brain networks. Methods: This study used EEG and functional network analysis, focusing on small-world propensity across various frequency bands (from δ to γ), to explore the global brain organization during the auditory discrimination of words and pseudowords in children with DD. Results: The main finding revealed a systemic inefficiency in the functional network of individuals with DD, which did not achieve the optimal small-world propensity. This inefficiency arises from a fundamental trade-off between localized specialization and global communication. During word listening, the δ-/γ1-networks (related to impaired syllabic and phonemic processing of words) and the θ-/β-networks (related to pseudoword listening) in the DD group showed lower local clustering and connectivity compared to the control group, resulting in reduced functional segregation. In particular, the θ-/β-networks for words in the DD group exhibited a less optimal balance between specialized local processing and effective global communication. Centralized midline hubs, such as the postcentral gyrus (PstCG) and inferior frontal gyrus (IFG), which are crucial for global coordination, attention, and executive control, were either absent or inconsistent in individuals with DD. Consequently, the DD network adopted a constrained, motor-compensatory, and left-lateralized strategy. This led to the redirection of information flow and processing effort toward the left PstCG/IFG loop, interpreted as a compensatory effort to counteract automatic processing failures. Additionally, the γ1-network, which is involved in phonetic feature binding, lacked engagement from posterior sensory hubs, forcing this critical process into a slow and effortful motor loop. The γ2-network exhibited unusual activation of right-hemisphere posterior areas during word processing, while it employed a simpler, less mature routing strategy for pseudoword listening, which further diminished global communication. Conclusions: This functionality highlights the core phonological and temporal processing deficits characteristic of dyslexia. Full article

Background/Objectives: Non-atherosclerotic pathological findings on coronary arteries involve various disorders that might lead to myocardial ischemia, independent of plaque complications and consequent lumen narrowing and obstruction. These patients often present with non-specific symptoms such as shortness of breath, rapid fatigue, and exertional chest tightness. When the underlying causes are non-atherosclerotic, these findings are frequently overlooked in radiology reports as a possible differential diagnosis. Therefore, the objective of this paper is to present the role of multidetector computed tomography (MD CT) coronary angiography in the diagnostic work-up of patients with rare but clinically valuable non-atherosclerotic pathological conditions of coronary arteries. Methods: We performed a literature search on Medline (via PubMed) for works presenting data on rare, non-occlusive, pathological findings on coronary arteries. Results: The review of the collected literature was performed in a narrative manner, intended to summarize mainly findings of imaging characteristics of non-occlusive pathologies: myocardial bridge, coronary aneurysm, ectasia, fistula, stenosis, and dissection. MD CT images of selected cases that were examined at our department, showing non-occlusive pathological changes in the coronary arteries, are displayed in planar and/or volume-rendered formats. Conclusions: Non-atherosclerotic abnormalities of the coronary vessel wall should be considered in the differential diagnosis of coronary causes of chest pain, dyspnea, and arrhythmias, as they may lead to both acute and chronic myocardial ischemia. Based on the presented literature and specific cases from our clinical practice, MD CT is shown to be an important tool for the rapid, non-invasive evaluation of non-atherosclerotic pathologies. Full article

Background: Healthcare workers (HCWs) have experienced sustained occupational exposure to SARS-CoV-2 throughout the COVID-19 pandemic. While infection prevention and control (IPC) practices have been widely implemented, limited prospective data exist on how occupational exposure risk and adherence to protective practices evolve over time, particularly beyond the acute phases of the pandemic. This study aimed to prospectively assess occupational and community exposure risk to COVID-19 among HCWs and to evaluate temporal changes in adherence to IPC practices during routine care and aerosol-generating procedures. Methods: A prospective observational study was conducted among HCWs from four public hospitals in the region of Thessaly, Greece. Eligible participants were HCWs who reported contact with suspected or confirmed COVID-19 cases. The data were collected at baseline (January–March 2022) and at a six-month follow-up using the World Health Organization’s “Risk Assessment and Management of Exposure of Health Care Workers in the Context of COVID-19” questionnaire. The instrument captured demographic characteristics, professional roles, occupational and community exposure, and adherence to IPC practices. Results: A total of 203 HCWs participated in the study. The overall proportion of HCWs reporting occupational exposure was 72.9% in both assessments. Among HCWs with occupational exposure (n = 148), the proportion classified as high-risk showed a statistically significant increase from 76% (95% CI: 0.6951–0.8320) at baseline to 88% (95% CI: 0.8258–0.9310) at follow-up (p = 0.010). This shift reflects a substantial effect size, with the odds of high-risk classification being more than double at follow-up (OR = 2.24). Nurses consistently demonstrated higher risk compared with physicians. The overall use of personal protective equipment remained high. However, adherence to several IPC practices declined over time, including removal and replacement of PPE according to protocol and frequent decontamination of high-touch surfaces. Conclusions: Occupational exposure risk among HCWs remained substantial and increased over time despite stable occupational exposure estimates. The observed decline in adherence to key IPC measures highlights the need for continuous monitoring and reinforcement of protective practices in healthcare settings. Full article

Background: Transcranial Direct Current Stimulation (tDCS) has shown potential in improving negative symptoms (NS) and Cognitive Impairment Associated with Schizophrenia (CIAS). However, heterogeneity in stimulation protocols and sample characteristics limit definitive conclusions regarding tDCS effectiveness in schizophrenia. Given the detrimental effects of cigarette smoking, particularly on cognition, this study explored the role of cigarette smoking as a modifiable individual factor potentially contributing to methodological heterogeneity by evaluating tDCS effects on NS and CIAS in Smoker (SM) and Non-Smoker (NoSM) patients. Methods: Post hoc analyses of a double-blind RCT were performed on 50 patients, randomized to 2 mA active or sham-tDCS (15 weekday sessions) with bilateral bipolar-nonbalanced prefrontal placement. The sample was divided according to the smoking status, consisting of 28 SM and 22 NoSM. Separate one-way analyses of covariance (ANCOVA) were performed within each subgroup to assess changes over time between treatment conditions. Clinical outcomes included Positive and Negative Symptoms Scale (PANSS), Brief Assessment of Cognition in Schizophrenia (BACS), Clinical Global Impression (CGI) and Calgary Depression Scale for Schizophrenia (CDSS) total scores. Results: SM exhibited baseline lower cognitive scores in verbal memory, motor speed and working memory domains. NS improved in both SM and NoSM with large effect size. Significant improvement in CIAS, specifically in working memory and verbal fluency, were found exclusively in NoSM. Conclusions: Cigarette smoking appeared to limit tDCS effectiveness in improving CIAS but not NS in schizophrenia. We suggested that the neurotoxic milieu linked to chronic exposure to neurotoxins of cigarette smoking could be responsible for these effects, counterbalancing the neuroprotective effects of tDCS. Further studies are warranted to replicate these findings. Full article

This study investigates the link between changes in physical activity (PA) measured by wearable accelerometers and the worsening of knee osteoarthritis (KOA) symptoms over two years. Using data from 782 participants in the Osteoarthritis Initiative accelerometer sub-study, PA was tracked with hip-worn ActiGraphs. Participants were classified as “worsening” if their Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) total score increased by >10 points and as “stable” otherwise. PA was categorized into daily counts and minutes spent in various intensity levels, and analyzed in 3 h intervals across the day. Of the participants, 123 (15.7%) experienced worsening symptoms. At baseline, both groups had similar characteristics aside from slower sit-to-stand times in the worsening group. Over two years, the worsening group had a greater decline in total daily activity counts (−18% vs. −10%) and more significant reductions during late afternoon and evening (15:00–21:00; −21% vs. −6%). This group also showed a notable decrease in gait speed, longer sit-to-stand times, and a trend towards greater medial joint space narrowing. These findings suggest that larger declines in PA, especially in activities in the late afternoon and evening, are associated with worsening KOA symptoms, although causality cannot be established. Full article

Background/Objectives: Staphylococcus aureus bloodstream infections (SABSIs) are associated with significant morbidity and mortality and are often complicated by infective endocarditis (IE). During the COVID-19 pandemic, fewer transesophageal echocardiographs (TEEs) were performed for patients diagnosed with SABSI, and this study examined the impact on clinical outcomes associated with this change in practice. Methods: This retrospective observational study included adult patients treated for SABSI who were admitted pre-COVID-19 (1 March 2018 to 11 March 2020) and during COVID-19 (12 March 2020 to 1 March 2022). Primary outcomes were rates of confirmed IE and duration of antibiotic therapy (DOT). Results: Of 333 screened patients, 214 were included (107 per group). Patients in the COVID-19 group were older (51.49 vs. 56.31 years, p = 0.013); other baseline characteristics were similar. Catheter-related infections were the most common source in the pre-COVID-19 and COVID-19 groups (30.8% vs. 18.9%, p = 0.089). Rates of TEE procedures significantly declined during COVID-19 (72% vs. 50.9%, p = 0.002); rate of confirmed IE (9.4% vs. 12.1%; p = 0.660) and median DOT (28 vs. 28 days; p = 0.596) were similar. Ninety-day mortality was higher in the COVID-19 group (10.4% vs. 22.2%, p = 0.019); other outcomes were not statistically different. Conclusions: The COVID-19 pandemic led to a notable decline in TEEs performed for SABSI, but the majority of clinical outcomes were unchanged. Mortality was significantly higher in the COVID-19 group, but it is uncertain that this was solely due to the change in practices. In a healthcare system that universally recommends TEE, scoring systems may help identify which patients are highest priority for TEE versus those that could undergo a transthoracic echocardiogram. Full article

This study addresses the issue of missing basic data and insufficient accuracy in predicting runoff and non-point-source pollution in the Heilongjiang region of China using the Soil and Water Assessment Tool (SWAT) model. Based on the China Ground Climate Data Daily Dataset (V3.0) and SPAW soil characteristic calculation formula, and assisted by the Python V3.0 language for data processing and computation, new high-precision weather generators and soil attribute databases suitable for the Heilongjiang region of China were established. The weather generator is based on daily data and contains detailed meteorological parameters such as temperature, humidity, wind speed, rainfall, etc., used to characterize the periodic changes in meteorological elements. And the differences and fluctuations outside this change curve were also retained in the basic construction of the weather generator. The soil database covers various parameters, such as soil type, texture, structure, nutrient content, organic matter content, etc., enabling the SWAT model to better simulate hydrological and pollutant transport processes in the soil. Additionally, point-source input data, including various industrial and domestic wastewater discharge situations, were collected and organized to improve data quality. Furthermore, a series of agricultural management measures were developed based on the use of fertilizers and pesticides for simulation, providing an important basis for analyzing non-point-source pollution using the SWAT model. By comparing the different results of the simulation using optimized databases, it is shown that the above work improved the simulation accuracy of the SWAT model in predicting runoff and pollution load in Heilongjiang, China. The NSE of runoff simulation increased from 0.923 to 0.988, and the NSE of ammonia nitrogen and CBOD simulation increased from 0.852 and 0.758 to 0.930 and 0.902, respectively. It is expected that these efforts will provide strong data support for subsequent research and provide a theoretical basis for government decision-makers to build scientifically rigorous and effective pollution control strategies. Full article

Background: Tau protein is a nonspecific marker of neurodegeneration, and its phosphorylated form, ptau-181, is specifically associated with Alzheimer’s disease (AD). Calculating the ratio of the phosphorylated form to total tau protein can help distinguish AD from other tauopathies or neurodegeneration, as well as reduce the impact of individual differences in total tau protein levels. This also allows one to monitor and compare the dynamics of changes within the same patient. Methods: Two SPRi biosensors were constructed, sensitive to the proteins described (total tau and ptau-181) for plasma determinations. The use of these biosensors requires prior sensor validation, during which specific parameters of the analytical method are established. Tests of the optimal concentration of the receptor layer in which particular antibodies were immobilized showed that the optimal concentration for total tau protein determinations was 1000 ng/mL. For ptau-181, it was 90 ng/mL. Biosensor layer formation was confirmed by analysis over a wide angle range, which enabled the generation of SPR curves. The dynamic range of the sensors is 1–50 pg/mL for total tau and 1–100 pg/mL for ptau-181. The limits of detection are 0.18 pg/mL and 0.037 pg/mL, respectively. Low standard deviation (SD) and coefficient of variation (CV) values indicate the good precision and accuracy of the results obtained using the SPRi biosensors. Specificity testing confirmed that no interferents influenced the assay. The method is therefore suitable for analyzing biological materials, such as blood plasma. Results: Proteins were thus measured in the blood plasma of AD patients and controls. Statistical analysis revealed significant differences in the concentrations of tau and ptau-181 protein between the two groups. The calculated ptau/total tau ratio for both sample groups also demonstrated high statistical significance. Conclusions: This suggests that a high ratio may be characteristic of AD. However, more extensive analysis is needed to obtain cutoff values. The ROC curves indicate that both biosensors have good diagnostic utility, with lower specificity for total tau. Full article