Search Results (121)

Cold stress limits the growth and productivity of Hevea brasiliensis, the primary source of natural rubber. This study investigated early transcriptomic responses in Reyan ‘7-33-97’ seedlings exposed to 4 °C, 10 °C, and 15 °C for 1, 2, and 4 h with room temperature (25 °C) as the control. RNA sequencing identified 9894 differentially expressed genes (DEGs), with the most significant transcriptional changes observed at 10 °C, indicating that genes to resist cold stress could not be mobilized at 4 °C, resulting in poor cold resistance of the rubber tree. KEGG enrichment analysis of DEGs between 10 °C (2 h) and 4 °C (2 h) revealed that genes involved in tryptophan metabolism (HbKynL.x1, HbKynL, HbCLP1, HbCLP2) and carbon metabolism (TCH4, XTH23), which contribute to cell wall modification, exhibited higher expression at 10 °C. Gene Ontology enrichment analysis highlighted significant involvement of “thylakoid,” “photosystems,” and “photosynthetic membrane,” alongside molecular functions such as “xyloglucan transferase activity” and “transcriptional regulator activity.” The interacting network of key pathways, including carbon metabolism (ko01200) and carbon fixation (ko00710) pathways, was sorted out, highlighting their integration with plant hormone signal transduction. Complex signaling networks, including MAPK, and kynurenine pathways coordinate the expression of cold-responsive genes and protective proteins, and it was confirmed and speculated that there is crosstalk response in cold defense mechanisms. Furthermore, 61 DEGs were associated with antioxidant processes, including major catalase and peroxidase enzymes. Our study shows that rubber trees physiological activities that respond to low-temperature signals cannot be carried out normally at 4 °C. The newly discovered metabolic pathway and the reason for abnormal cold signal transduction at low temperatures are the focus of future research on cold resistance. Full article

This study presents the results of preliminary documentation and radon tracer investigations conducted at the “Aurelia” Mine in Złotoryja. Measurements of ²²²Rn activity concentrations were carried out between 17 March and 26 August 2023, while terrestrial laser scanning (TLS) for mapping purposes was performed on 16 November 2024. The radon data exhibited a consistently right-skewed distribution, with skewness coefficients ranging from 0.9 to 8.2 and substantial standard deviations, indicating significant data dispersion. Outliers and extreme outliers were identified as key factors influencing average radon activity concentrations from April through August, whereas data from March displayed homogeneity, with no detected anomalies. The average ²²²Rn activity concentrations recorded from March to July ranged from 51.4 Bq/m³ to 65.9 Bq/m³. In contrast, July and August showed elevated average values (75.8 Bq/m³ and 5784.8 Bq/m³, respectively) due to the presence of outliers and extreme values. Upon removal of these anomalies, the adjusted means were 73.8 Bq/m³ and 1003.6 Bq/m³, respectively, resulting in reduced skewness and improved representativeness. These findings suggest that the annual average radon concentrations at the “Aurelia” Mine remain compliant with the regulatory threshold of 300 Bq/m³ set by the Atomic Law Act, with exceedances likely related to atypical or rare geophysical phenomena requiring further statistical validation. August exhibited a significant occurrence of outliers and extreme outliers in ²²²Rn activity concentration data, particularly concentrated between the 13th and 17th days of the month. This anomaly is hypothesized to be associated with geological processes, notably mining-induced seismic events within the LGOM (Legnica–Głogów Copper District) region. It is proposed that periodic transitions between tensional and compressional phases within the rock mass, triggered by mining activity, may lead to abrupt increases in radon exhalation, potentially occurring before or after seismic events with a magnitude exceeding 2.5. Although the presented data provide preliminary evidence supporting the influence of tectonic kinematic changes on subsurface radon dynamics, further systematic observations are required to confirm this relationship. At the current stage, the hypothesis remains speculative but may contribute to the broader understanding of radon behavior in geologically active underground environments. Complementing the geochemical analysis, TLS enabled detailed geological mapping and 3D spatial modeling of the mine’s underground tourist infrastructure. The resulting simplified linked data model—integrating radon activity concentrations, geological structures, and spatial parameters—provides a foundational framework for developing a comprehensive GIS database. This integrative approach highlights the feasibility of combining tracer studies with spatial and cartographic data to improve radon risk assessment models and ensure regulatory compliance in underground occupational settings. Full article

The soil nitrification rate is significantly affected by plant species, and it is also modulated by different nitrogen levels in the soil. There are a wide range of plant species with the capacity to produce biological nitrification inhibitors (hereafter referred to as BNI species). The preliminary results of this study report the influence of three different plant species on the nitrification rates under soil supply with three (0 mM, 3.5 mM, and 7.0 mM) nitrogen levels. The aim was to evaluate the potential of hemp, ryegrass, and sorghum in mitigating nitrification, in order to define a sustainable strategy for improving the nitrogen use efficiency by crops and to limit the nitrogen loss from agroecosystems. Leaf gas exchange measurements were also carried out in this study. Photosynthesis was only affected by nitrogen supply in hemp, resulting in a reduction in CO₂ assimilation at nitrogen doses higher than the plant’s requirements. Ryegrass devotes more reductive power towards leaf nitrogen assimilation than sorghum and hemp do. The greatest variation in nitrification rate in response to N was observed in soil cultivated with hemp (which also showed the highest potential nitrification rate), followed by sorghum and ryegrass. We speculate that this occurred because the greater seed sowing density for ryegrass ensured a greater quantity in the soil of molecules acting on nitrification compared to sorghum and hemp, with these latter being sown at lower densities. Our results suggest that sorghum and ryegrass might directly affect nitrification by BNI molecules, whereas hemp might indirectly mitigate nitrification through the nitrogen uptake. However, further research is needed to evaluate the effects exerted by the studied plant species on nitrification rates. Full article

Background: Polymyalgia rheumatica (PMR) has a multifaceted onset and course, and making a distinction between true PMR and so-called “polymyalgic syndrome” (that is, similar manifestations caused by different conditions) is far from easy in clinical practice. The existence of subsets within true PMR may further complicate the diagnostic question. Distinguishing PMR subsets from PMR-mimicking conditions does not just carry nomenclature value and speculative significance. Indeed, the correct diagnosis influences treatment, prognosis, epidemiological assessments, and health policies. Objectives: We aimed to (1) ascertain the presence of a definite and peculiar subset/subgroup/cluster of PMR in the scientific literature; (2) describe any possible subset/cluster/subgroup of PMR identified in at least two different studies. Methods: We performed a non-systematic (PRISMA protocol not followed) literature search on Embase and Medline (OVID interface). The following search terms were used: polymyalgia rheumatica, subset, cluster, subgroup, subclinical giant cell arteritis, mimicking conditions, polymyalgia rheumatica-like conditions, immunotherapy, checkpoint inhibitor, acute-phase reactants or acute-phase proteins, vaccination, infection, and calcium pyrophosphate deposition disease or chondrocalcinosis. Each paper’s reference list was scanned for additional publications meeting this study’s aim. Abstracts submitted at conferences or from non-peer-reviewed sources were not included. Results: The initial search yielded 2492 papers, of which 2389 articles were excluded based on title and abstract screening. A total of 103 articles underwent a full-length review, and 84 of them were finally assessed for eligibility. A total of seven large subsets of PMR could be identified: (1) PMR with normal acute-phase reactants; (2) PMR with an infection trigger; (3) PMR with a vaccination trigger; (4) PMR with subclinical giant cell arteritis (GCA); (5) PMR and calcium pyrophosphate deposition disease (CPPD); (6) PMR following immune checkpoint inhibitor (ICI) therapy; (7) PMR with peculiar clinical clusters (based on clinical or statistic clustering methods). Conclusions: PMR with normal baseline acute-phase reactants and PMR with an infection or a vaccination trigger could be categorized as subsets of disease. PMR with subclinical GCA and most cases of PMR/CPPD should be categorized as mimickers. Finally, further studies are required to better categorize some peculiar clinical subsets emerging from cluster analyses, and ICI-induced PMR. Full article

Sufficient protein intake has been shown to be advantageous for developing muscle mass, muscle strength, muscle quality, as well as for improving recovery from strenuous exercise, all of which are essential for athletic performance. Aging athletes, so-called master athletes, would benefit from evidence-based recommendations; however, studies investigating the role of their protein intake on muscle quality or performance are diverse and rare. Consequently, existing recommendations for this subpopulation of aging athletes are non-existent or speculative in nature. The aim of this short scoping review is to summarize available reports, identify common outcomes, and suggest future research directions. Literature research was carried out in PubMedMedline, SPORTDiscuss, and ScienceDirect without any restrictions regarding year of publication, type of research or sports discipline. Only observational and interventional studies with data on protein intake linked with body composition or performance outcomes were included for further analyses. We identified 12 suitable reports on master athletes with very diverse age-ranges, a broad variety of sports, and very heterogeneous outcome parameters. Seven studies investigated endurance athletes, four studies investigated multi-sports athletes, and only one study reported on strength-trained athletes. Average protein intake ranged between 1.0 and 1.9 g/kg/d. Within the few available studies, evidence tends to point towards a benefit of higher protein intakes for muscle mass and function; however, the low number of studies, combined with heterogeneity in study design and methods, limits their generalizability. Future studies are needed to build the evidence base for clear dietary recommendations respecting the specific needs of aging athletes. Full article

Tourist activity has a powerful capacity to transform landscapes, often modifying natural or semi-natural spaces in favour of increasingly dense urban structures. This change is particularly evident in Mediterranean coastal areas, and specifically in Spain’s La Manga del Mar Menor in the Region of Murcia, whose environment and landscape have been altered from the 1960s onwards. During this time, intense urbanisation based on the sun and beach tourism model has been developed, which represents a bad example of urban land-use planning. Speculation and the lack of a sustainable tourist model in those years have created a 22-km linear city along a coastal strip, currently characterised by a densified tourist area. This situation is due to the lack of urban planning and a series of other problems, such as the lack of basic services and facilities, saturation in access to them and environmental pollution. In this spatial context, the geography of perception’s methodology will serve as an analytical tool in aspects related to the lived space and the interaction of residents and tourists. This is combined with a DAFO/SWOT-CAME analysis and the use of photographs and cartographies as key research instruments. The aim of this work is to analyse the intense urbanisation that has taken place in this coastal area and to show the need to carry out urban development actions within the framework of a possible new plan aimed at halting environmental deterioration, organising land use, creating more spaces for public services and protecting, to a greater extent, beaches and other natural and cultural resources. A commitment to sustainable tourism should ensure better urban conditions to meet both visitor and local demands. Full article

Dioryctria abietella Denis Schiffermuller (Lepidoptera: Pyralidae) is an oligophagous pest that mainly damages Pinaceae plants. Here, we investigated the effects of the Bacillus thuringiensis 2913 strain (Bt 2913), which carries the Cry1Ac, Cry2Ab, and Vip3Aa genes, on the D. abietella midgut transcriptome at 6, 12, and 24 h after infection. In total, 7497 differentially expressed genes (DEGs) were identified from the midgut transcriptome of D. abietella larvae infected with Bt 2913. Among these DEGs, we identified genes possibly involved in Bt 2913-induced perforation of the larval midgut. For example, the DEGs included 67 genes encoding midgut proteases involved in Cry/Vip toxin activation, 74 genes encoding potential receptor proteins that bind to insecticidal proteins, and 19 genes encoding receptor NADH dehydrogenases that may bind to Cry1Ac. Among the three transcriptomes, 88 genes related to metabolic detoxification and 98 genes related to immune defense against Bt 2913 infection were identified. Interestingly, 145 genes related to the 60S ribosomal protein were among the DEGs identified in the three transcriptomes. Furthermore, we performed bioinformatic analysis of zonadhesin, GST, CYP450, and CarE in the D. abietella midgut to determine their possible associations with Bt 2913. On the basis of the results of this analysis, we speculated that trypsin and other serine proteases in the D. abietella larval midgut began to activate Cry/Vip prototoxin at 6 h to 12 h after Bt 2913 ingestion. At 12 h after Bt 2913 ingestion, chymotrypsin was potentially involved in degrading the active core fragment of Vip3Aa toxin, and the detoxification enzymes in the larvae contributed to the metabolic detoxification of the Bt toxin. The ABC transporter and several other receptor-protein-related genes were also downregulated to increase resistance to Bt 2913. However, the upregulation of 60S ribosomal protein and heat shock protein expression weakened the resistance of larvae to Bt 2913, thereby enhancing the expression of NADH dehydrogenase and other receptor proteins that are highly expressed in the larval midgut and bind to activating toxins, including Cry1Ac. At 24 h after Bt 2913 ingestion, many activated toxins were bound to receptor proteins such as APN in the larval midgut, resulting in membrane perforation. Here, we clarified the mechanism of Bt 2913 infection in D. abietella larvae, as well as the larval immune defense response to Bt 2913, which provides a theoretical basis for the subsequent control of D. abietella using B. thuringiensis. Full article

The determinants and driving forces of communication abilities in the locked-in state are poorly understood so far. Results from an experimental–clinical study on a completely paralyzed person involved in communication sessions after the implantation of a microelectrode array were retrospectively analyzed. The aim was to focus on the prerequisites and determinants for learning to control a brain–computer interface for communication in paralysis. A comparative examination of the communication results with the current literature was carried out in light of an ideomotor theory of thinking. We speculate that novel skill learning took place and that several aspects of the wording of sentences during the communication sessions reflect preserved cognitive and conscious processing. We also present some speculations on the operant learning procedure used for communication, which argues for the reformulation of the previously postulated hypothesis of the extinction of response planning and goal-directed ideas in the completely locked-in state. We highlight the importance of feedback and reinforcement in the thought–action–consequence associative chain necessary to maintain purposeful communication. Finally, we underline the necessity to consider the psychosocial context of patients and the duration of complete immobilization as determinants of the ‘extinction of thinking’ theory and to identify the actual barriers preventing communication in these patients. Full article

Silicone rubber (SR) is a kind of polymer insulation material with excellent performance. With the service life of silicone rubber products reaching the limit, how to dispose of waste silicone rubber is an urgent problem to be solved. In this paper, silicone rubber-modified asphalt binder (SRMA) was prepared by SR and 90# base asphalt binder. The simulated short-term aging and long-term aging tests of SRMA were carried out using the thin film oven aging test (TFOT) and pressure aging vessel test (PAV). The rotary viscosity test and dynamic shear rheological test (DSR) were applied to the rheological properties of SRMA before and after aging. The degradation degree and chemical composition changes of SR were explored by the toluene insoluble matter test, Fourier transform infrared spectroscopy (FTIR), and a Fluorescence microscope (FM). The results demonstrate that SR can significantly affect the aging resistance, fatigue life, and high-temperature stability of SRMA. As the content of SR rose, the elastic component in SRMA increased, leading to a nice performance in stability at high temperatures and fatigue resistance. However, excessive content (14%wt and 16%wt) had a negative influence on the performance of SRMA. So, the optimal content was speculated to be between 12% and 14%. Furthermore, SR and asphalt binder would be aged and degraded together in the aging process, and this phenomenon was more obvious during long-term aging. Full article

Background: Sarcopenia is an age-related condition characterized by progressive loss of muscle mass, strength, and function. The occurrence of sarcopenia has a huge impact on physical, psychological, and social health. Therefore, the prevention and treatment of sarcopenia is becoming an important public health issue. Method: 35 six-week-old male C57BL/6 mice were randomly divided into five groups, one of which served as a control group, while the rest of the groups were constructed as a model of sarcopenia by intraperitoneal injection of D-galactose. The intervention with lactoferrin, creatine, and their mixtures, respectively, was carried out through gavage for 8 weeks. Muscle function was assessed based on their endurance, hanging time, and grip strength. The muscle tissues were weighed to assess the changes in mass, and the muscle RNA was extracted for myogenic factor expression and transcriptome sequencing to speculate on the potential mechanism of action by GO and KEGG enrichment analysis. Result: The muscle mass (lean mass, GAS index), and muscle function (endurance, hanging time, and grip strength) decreased, and the size and structure of myofiber was smaller in the model group compared to the control group. The intervention with lactoferrin and creatine, either alone or combination, improved muscle mass and function, restored muscle tissue, and increased the expression of myogenic regulators. The combined group demonstrated the most significant improvement in these indexes. The RNA-seq results revealed enrichment in the longevity-regulated pathway, MAPK pathway, focal adhesion, and ECM–receptor interaction pathway in the intervention group. The intervention group may influence muscle function by affecting the proliferation, differentiation, senescence of skeletal muscle cell, and contraction of muscle fiber. The combined group also enriched the mTOR-S6K/4E-BPs signaling pathway, PI3K-Akt signaling pathway, and energy metabolism-related pathways, including Apelin signaling, insulin resistance pathway, and adipocytokine signaling pathway, which affect energy metabolism in muscle. Conclusions: Lactoferrin and creatine, either alone or in combination, were found to inhibit the progression of sarcopenia by influencing the number and cross-sectional area of muscle fibers and muscle protein synthesis. The combined intervention appears to exert a more significant effect on energy metabolism. Full article

This study demonstrates a failure identification methodology applied to a preprocessing facility generating conversion-ready feedstocks from biomass meeting conversion process critical quality attribute (CQA) specifications. Failure Modes and Effects Analysis (FMEA) was used as an industrially relevant risk analysis approach to evaluate a logging residue preprocessing system to prepare feedstock for pyrolysis conversion. Risk evaluations considered both system-level and operation unit-level assessments considering process efficiency, product quality, cost, sustainability, and safety. Key outputs included estimations of semi-quantitative risk scores for each failure, identification of the failure impacts, identification of failure causes associated with material attributes and process parameters, ranking success rates of failure detection methods, and speculation of potential mitigation strategies for decreasing failure risk scores. Results showed that deviations from moisture specifications had cascading consequences for other CQAs along with process safety implications. Failures linked to fixed carbon specifications carried the highest risk scores for product quality and process efficiency impacts. As increased throughput can be inversely related to meeting product quality specifications; achieving throughput and other material-based CQAs simultaneously will likely require system optimization or prioritization based on system economics. Ultimately, this work successfully demonstrates FMEA as a risk analysis approach for other bioenergy process systems. Full article

The syndrome of heart failure (HF) with preserved ejection fraction (HFpEF) makes up about half of the HF population. The HF mechanisms in these patients are varied and not fully understood. In addition, the term “diastolic HF” was switched to HFpEF because of difficulties in measuring the left ventricular (LV) diastolic performance. In the late stages, HFpEF carries a prognosis that is as bad as or worse than that of HFrEF. Hence, it is important to recognize LV diastolic impairment at an earlier stage so that the causal mechanisms, if any, can be treated to retard its progression. Despite the availability of numerous disease-modifying agents for HFrEF, there are hardly any available treatments for HFpEF. With our aging population, there will be an epidemic of HFpEF and hence this entity needs attention and respect. In this paper, we review the fundamental mechanisms of HFpEF, the physiology of LV filling and how LV diastolic function can be comprehensively measured. We also speculate how this may help with the early recognition of diastolic HF and its treatment. Full article

Refractory acute myeloid leukaemia is very difficult to treat and represents an unmet clinical need. In recent years, new drugs and combinations of drugs have been tested in this category, with encouraging results. However, all treated patients relapsed and died from the disease. The only curative option is allogeneic transplantation through a graft from a healthy donor immune system. Using myeloablative conditioning regimens, the median overall survival regimens is 19%. Several so-called sequential induction chemotherapies followed by allogeneic transplantation conditioned by reduced intensity regimens have been developed, improving the overall survival to 25–57%. In the allogeneic transplantation field, continuous improvements in practices, particularly regarding graft versus host disease prevention, infection prevention, and treatment, have allowed us to observe improvements in survival rates. This is true mainly for patients in complete remission before transplantation and less so for refractory patients. However, full myeloablative regimens are toxic and carry a high risk of treatment-related mortality. In this review, we describe the results obtained with the different modalities used in more recent retrospective and prospective studies. Based on these findings, we speculate how allogeneic stem cell transplantation could be modified to maximise its therapeutic effect on refractory acute myeloid leukaemia. Full article

Introduction: Depression is a very common psychological problem observed all around the world. Though various treatment modalities are available for this mental illness, further research is warranted to find novel therapeutics. Recent speculations on the role of adiponectin in animal models of depression have shown interesting results. Leptin is an adipokine that affects mood and cognition. Hence, the role of adiponectin and leptin in patients with depression needs to be probed. Objective: To estimate serum adiponectin and leptin concentration in depressive patients and compare them with healthy controls. Methods: This study was conducted at the Department of Biochemistry and Psychiatry, VMMC, and Safdarjung Hospital, New Delhi, following ethical clearance from our institution’s Ethics Committee. Serum samples were taken from 30 severely depressive patients and 30 healthy controls subsequent to receiving written consent from the patients’ relatives. The samples were checked for serum adiponectin and leptin levels via QUAEE-BIO and a DBC ELISA kit. Data were analyzed using SPSS version 21.0. Results: Cases and controls were age- and sex-matched. Serum adiponectin levels in the depression group were significantly higher than in the controls [Z = −2.18, p = 0.03]. Serum leptin levels in the depression group were not significantly different from that of the controls [Z = −0.47, p = 0.64]. Also, there was no correlation between serum adipokine levels and the severity of depression. However, there was a significant difference in adipokine levels between sexes in the case group and in the whole study group (p = 0.000). Conclusions: The higher serum adiponectin levels in the case group are in contrast with previous studies, which found lower adiponectin levels in depression. This may be due to the effect of treatment in cases of depression; further large-scale studies should be carried out to elucidate the role of adiponectin in depression. The small study size and absence of data about pretreatment serum adiponectin levels are the major limitations of this study. Full article

The purple sulfur bacterium Thiocapsa roseopersicina BBS is interesting from both fundamental and practical points of view. It possesses a thermostable HydSL hydrogenase, which is involved in the reaction of reversible hydrogen activation and a unique reaction of sulfur reduction to hydrogen sulfide. It is a very promising enzyme for enzymatic hydrogenase electrodes. There are speculations that HydSL hydrogenase of purple bacteria is closely related to sulfur metabolism, but confirmation is required. For that, the full genome sequence is necessary. Here, we sequenced and assembled the complete genome of this bacterium. The analysis of the obtained whole genome, through an integrative approach that comprised estimating the Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (DDH) parameters, allowed for validation of the systematic position of T. roseopersicina as T. bogorovii BBS. For the first time, we have assembled the whole genome of this typical strain of a new bacterial species and carried out its functional description against another purple sulfur bacterium: Allochromatium vinosum DSM 180T. We refined the automatic annotation of the whole genome of the bacteria T. bogorovii BBS and localized the genomic positions of several studied genes, including those involved in sulfur metabolism and genes encoding the enzymes required for the TCA and glyoxylate cycles and other central metabolic pathways. Eleven additional genes coding proteins involved in pigment biosynthesis was found. Full article