Search Results (17,239)

Blockchain technology (BCT) is often discussed as digital support for timber traceability in the context of the EU Deforestation-Free Products Regulation (EUDR), which requires operators and traders to demonstrate legality, deforestation-free production, geolocation at the forest parcel level, and verifiable supply chain documentation from 30 December 2026, with an extended timeline for micro, small, and medium-sized enterprises (SMEs) until 30 June 2027. This study assesses where BCT can realistically add value in timber supply chains under operational forestry conditions and identifies the necessary technical, organizational, and legal prerequisites. A combination of a targeted literature review and empirical input from experts in the forestry and timber industry, including guided expert web-conferencing interviews (n = 41), an online survey (n = 69 completed responses), and a transdisciplinary workshop (n = 18) was utilized to obtain a comprehensive overview of industry perspectives. Qualitative data from interviews and workshop sessions were analyzed using structured qualitative content analysis, while survey data were evaluated using descriptive statistics. The expected benefits are associated with the introduction of tamper-proof timber harvesting practices and cross-organizational verification mechanisms. To address the discrepancy between biological uncertainty and digital rigidity, the study proposes a dynamic allocation model adapted from the energy sector that distinguishes between fixed and variable wood capacities to automate logistical planning via smart contracts. However, respondents emphasize that practical obstacles, such as limited digital maturity in forestry, fragmented data infrastructures across the supply chain, and unresolved issues of data sovereignty hinder the implementation of BCT. BCT alone is unable to resolve the problem of weak physical-digital identity continuity, a phenomenon widely known as the oracle problem; however, coupling the ledger with physical or biological anchors (e.g., photo-optical, automated inkjet marking identification) can re-establish this physical–digital continuity and thereby resolve the oracle problem. The results demonstrate that blockchain acts most plausibly as a supporting component within hybrid traceability architectures that prioritize event-based authentication, off-chain data processing where appropriate, and integration with existing certification systems. The study highlights the necessity of defining distinct organizational roles and responsibilities while integrating user-centric digital solutions tailored specifically to small and medium-sized enterprises (SMEs). Full article

This introduction accompanies nine articles published in this Special Issue of Genealogy titled “Adoption Is Stranger than Fiction,” over the course of 2025–2026. It draws out common themes and methodologies and reflects on the enduring appeal of identity quests and rootwork. It posits the more productive concept of identity-in-agency, the notion of the continuous development of one’s identity through active work in adoption-related areas, whether through activism, teaching, researching, or committing to literary and cultural activities. Lastly, this introduction reckons with voices denouncing the adoptee’s search for identity, often presented as an overly biological or biogenetic interest that discounts identity components that have been determined by culture or environment, not by birth or genes. Full article

Phytol, an acyclic diterpene alcohol and a key lipophilic side-chain moiety of chlorophyll, is widely distributed in nature. It exhibits potent antioxidant, anti-inflammatory, analgesic and broad-spectrum antibacterial activities. Notably, phytol can also effectively inhibit melanin production, repair the skin barrier, and exert profound anti-aging effects, making it a highly promising ingredient for daily skincare with substantial industrial application value. The skincare benefits of phytol are primarily achieved by constructing a multi-dimensional regulatory network involving defense, modulation and repair. Compared to conventional retinol-based skincare ingredients, phytol exhibits superior biocompatibility, mild irritation, and remarkable safety advantages. Nevertheless, its application is hindered by inherent limitations, including strong hydrophobicity, spontaneous aggregation tendency, and poor photothermal stability. These drawbacks severely restrict its dispersibility, storage stability and percutaneous bioavailability in aqueous cosmetic formulations. To address these deficiencies, nanodrug delivery systems (NDDS), such as liposomes, nanoemulsions, solid lipid nanoparticles and PLGA nanoparticles, have been widely employed. These nanocarriers can penetrate the skin barrier via the size effect, enabling targeted skin delivery and long-term controlled release of phytol. This review systematically summarizes the biological sources and metabolic fate of phytol, as well as its multi-mechanistic pharmacological effects on the skin. Furthermore, we outline the current application status and industrial development trends of phytol in mainstream cosmetics worldwide. This work aims to provide theoretical basis and forward-looking references for the development of high-efficiency, safe and stable phytol-derived skincare raw materials and topical formulations. Highlights: (1) Phytol, a natural acyclic diterpene alcohol, exerts multi-dimensional skincare effects including antioxidant, anti-inflammatory, whitening, anti-aging, and skin barrier repair activities via a defense–modulation–repair regulatory network. (2) Phytol may act as a mild, non-irritating functional alternative to retinoids, targeting PPAR/RXR pathways and avoiding TRPV1-mediated irritation, making it suitable for sensitive skin. (3) Poor water solubility and instability hinder phytol’s translation; nanodelivery systems effectively improve solubility, permeability, and sustained release. Full article

Background/Objectives: Type 2 diabetes mellitus (T2DM) is a multifactorial metabolic disorder characterized by chronic hyperglycemia, oxidative stress, protein glycation, low-grade inflammation, and dysregulation of signaling pathways, including those involving nuclear factor kappa B (NF-κB). Isoflavones from Fabaceae species exhibit diverse biological activities and may represent multifunctional candidates for addressing the interconnected mechanisms underlying T2DM. This study performed a consensus in silico pharmacodynamic screening of twelve representative Fabaceae isoflavones to prioritize compounds with predicted antiglycating, deglycating, antidiabetic, antioxidant, and NF-κB-related anti-inflammatory activities. Methods: Twelve representative Fabaceae isoflavones were evaluated using a multimethod computational workflow. Antiglycating and deglycating activities were predicted using IT Microcosm, antidiabetic and hypoglycemic activities were assessed using Prediction of Activity Spectra for Substances (PASS), antioxidant potential was evaluated using similarity-based prediction and quantum-chemical calculations, and NF-κB-related anti-inflammatory activity was investigated using similarity-based prediction combined with molecular docking. Molecular docking was applied specifically to the NF-κB-related anti-inflammatory analysis and did not contribute to the hypoglycemic/antidiabetic prediction. The individual predictions were integrated using a consensus scoring strategy to prioritize compounds across multiple pharmacodynamic domains. Results: The investigated isoflavones demonstrated moderate but broad predicted multifunctional activities. Malonylgenistin, biochanin A, and formononetin showed the most favorable predicted antiglycating profiles. Genistin, acetyldaidzin, acetylglycitin, and puerarin ranked highest in terms of predicted antidiabetic activity, whereas genistein exhibited the strongest predicted antioxidant profile. Daidzin and genistin received the highest integrated NF-κB-related computational rankings, although these predictions apply to intact glycosides rather than their expected in vivo hydrolysis products. Conclusions: This study presents a consensus computational framework for the comparative prioritization of Fabaceae isoflavones based on their predicted multifunctional pharmacodynamic profiles. The results provide a rational basis for selecting candidate compounds for future biochemical, cell-based, pharmacokinetic, and in vivo investigations, although it is emphasized that the present findings are based exclusively on computational predictions and require experimental validation. Full article

The use of different marine resources, including various colouring agents, is rooted in human history. They have been used as nutrients and medicaments and in luxury products. Readily available marine biological pigments (MBPs) are considered inexpensive materials, since they can be used as colourants. MBPs form part of the ‘’blue technology’’ approach for industrial colouring applications, including staining, textile dyeing, and uses to give fashionable colours to luxury products. They serve as nutrient additives, cosmetic ingredients, and as parts of beauty products. Today, they still are broadly used in the context of diverse new applications. MBPs can be concentrated differently on the bodies of marine creatures, providing each pigment its unique colour and properties. MBPs like carotenoids can complement important cell activities, like photosynthesis. They can function independently or support other micro- and macromolecules. MBPs are essential for their host’s survival. Outside their primary hosts (in the context of uses on and in consumers’ corpora), they either stay unmodified or can be changed by association with specific micro- and macromolecules. A few types of MBPs have been extracted, purified, identified, and formulated as drugs or pure chemicals. MBPs share common properties, such as functioning as antioxidants, can protect against sunlight and UV waves, and can improve vision. They take part in health protection and disease treatment, including anticancer, anti-inflammatory, anti-neurodegeneration, anti-ageing, and anti-wrinkle functions, and can function as an antimicrobial. With many undiscovered properties, MBPs represent a source for de novo applications with innumerable chances that might offer mastery of the fields of colour-based applications. This review summaries the importance of MBPs and addresses important applicable properties that make them attractive for nutraceutical, medicinal, pharmaceutical, and cosmeceutical uses, in addition to various industrial applications, and discusses historical and contemporary facts that have attracted human attention, both in the past and today, along with setting out expectations for the future of MBPs. Full article

Background/Objectives: Veterinary vaccine manufacturing is essential for controlling transboundary animal diseases, strengthening animal health systems, and supporting livestock development, particularly in Southeast Asia where livestock production continues to expand. However, modernization toward Pharmaceutical Inspection Co-operation Scheme (PIC/S) Good Manufacturing Practice (GMP) compliance remains challenging because it requires coordinated development across regulatory, technical, organizational, financial, and governance domains. This study aimed to develop an evidence-informed strategic framework for veterinary vaccine manufacturing modernization through a structured and systematically documented framework development methodology integrating multidisciplinary evidence and practice-informed implementation experience. Methods: An evidence-informed framework development study was conducted through the multidisciplinary integration of multiple evidence sources, including international regulatory guidance, peer-reviewed scientific literature, multidisciplinary feasibility assessment, and practice-informed implementation experience derived from a government-commissioned consultancy project. Framework development followed a structured methodology comprising evidence collection, evidence categorization, multidisciplinary evidence synthesis, framework development, iterative refinement, and expert-informed validation. Only generalized methodological principles and potentially transferable implementation knowledge were incorporated to ensure confidentiality of project-specific information. Results: Multidisciplinary evidence synthesis identified ten evidence domains encompassing regulatory, technical, organizational, economic, legal, environmental, governance, workforce, infrastructure, and implementation considerations. These domains were translated through a structured process into an evidence-informed strategic framework organized around four sequential modernization phases—Design, Construction, Validation, and Continuous Improvement—supported by cross-cutting strategic enablers. Iterative refinement through expert review, technical consultation, and implementation verification strengthened the framework’s scientific consistency, regulatory alignment, and practical applicability. Conclusions: Veterinary vaccine manufacturing modernization should be regarded as a multidisciplinary organizational transformation rather than solely as an infrastructure or engineering project. Beyond proposing a strategic framework, this study provides a transparent, systematically documented and traceable methodology for translating multidisciplinary evidence into strategic decision-support tools. The proposed framework may assist governments, regulatory authorities, vaccine manufacturers, and researchers in developing evidence-informed modernization strategies for veterinary biological manufacturing systems, particularly in low- and middle-income countries, subject to adaptation to specific regulatory, technical, institutional, and resource contexts. Full article

Collagen functional fragments retain selected activities of parent collagens while allowing greater flexibility in sequence design and precision fermentation. Although recent reviews have covered recombinant collagen production technologies, expression platforms, purification strategies, quality control, and biomedical applications, fragment selection, host–process matching, production, and characterization have received less integrated attention. This review focuses primarily on collagen-derived functional fragments, while collagen-mimetic peptides and collagen-like proteins are discussed as related design systems. The biological basis for fragmentation includes receptor-recognition motifs, matrikines and matricryptins, and basement membrane-derived fragments. The review further examines how motif context, Gly-X-Y organization, stabilizing sequence features, protease susceptibility, post-translational modification requirements, and host compatibility influence fragment stability, expression performance, production feasibility, and product integrity. Microbial production using Escherichia coli, Komagataella phaffii, and Saccharomyces cerevisiae is discussed from the perspectives of construct–host matching, secretory or intracellular production, prolyl 4-hydroxylase configuration, fermentation optimization and scale-up, and product characterization. Finally, we discuss AI-assisted, quality-guided design-build-test-learn workflows that integrate computational prediction, curated structural, extracellular-matrix, interaction, and protease resources, two-tier candidate evaluation, and format-appropriate experimental testing to support iterative sequence, host, and process optimization. The development of collagen functional fragments therefore depends on coordinated optimization of biological function, molecular design, microbial host performance, fermentation processes, and product characterization. Full article

Tetracycline (TC) is a widely used antibiotic that is frequently detected in rivers, lakes and wastewater. Because TC is poorly removed by conventional biological treatment, its residues can harm aquatic organisms and promote the spread of antibiotic resistance; efficient and low-cost technologies for removing TC from water are therefore needed. In this study, copper smelting slag (CSS), an abundant industrial solid waste, was converted into a catalyst composed of Fe₃O₄ particles loaded on mesoporous silica (denoted Fe₃O₄@MS) via an alkali fusion–hydrothermal method. The catalyst was used to activate peroxymonosulfate (PMS), forming the Fe₃O₄@MS/PMS treatment system for the degradation of TC in aqueous solution. The effects of the main operating parameters (catalyst dosage, PMS concentration, initial pH and reaction temperature) on TC degradation were systematically evaluated. Under the optimized conditions (catalyst 0.5 g/L, PMS 1.0 mmol/L, initial pH 6.5, 25 °C), the Fe₃O₄@MS/PMS system removed 98.70% of 50 mg/L TC within 60 min. Radical quenching experiments and electron paramagnetic resonance (EPR) analysis revealed that TC was degraded through both radical pathways (hydroxyl •OH, sulfate SO₄^•− and superoxide O₂^•− radicals) and a non-radical pathway involving singlet oxygen (¹O₂), with •OH being the dominant reactive species. Nine degradation intermediates were identified by liquid chromatography–mass spectrometry (LC-MS), based on which three degradation pathways were proposed. Toxicity estimation indicated that ring-opening and deamination reactions are the key steps for detoxification. In addition, a life cycle assessment (LCA) across five selected impact categories identified the main environmental burdens associated with catalyst production. Overall, this work demonstrates that CSS-derived Fe₃O₄@MS is an efficient, low-cost and sustainable catalyst for PMS-based antibiotic removal from water, offering a circular-economy approach that couples solid-waste valorization with clean water production. Full article

Filamentous fungi are ubiquitous eukaryotic microorganisms characterized by multicellular architectures and branched hyphal structures. They are used in industrial production for the manufacture of enzyme preparations, various organic acids, and secondary metabolites. Transcription factors serve as core regulatory molecules governing vital biological processes in filamentous fungi. This review systematically elaborates on the classification, structural features, and functional diversity of transcription factors in filamentous fungi, with a focus on their regulatory roles in hyphal growth, morphological differentiation, metabolite synthesis, and environmental stress responses. We further summarize the key transcription factors in three representative fungal genera, namely, Aspergillus, Trichoderma, and Penicillium. Additionally, the potential applications of genetically engineering transcription factors to increase the yield of metabolites are discussed. This review provides a comprehensive theoretical framework for the genetic modification and high-value utilization of filamentous fungi. Full article

Background/Objective: One of the missions of the United States FDA is “protecting the public health by ensuring the safety, efficacy, and security of human and veterinary drugs, biological products.” The current guidelines for OTC hearing aids impose an output limit of 111 dB SPL for linear hearing aids and 117 dB SPL for compression hearing aids when measured using 90 dB SPL pure tones in a 2 cc coupler. This means that the sound pressure level at the ear drum at a frequency would be 111 or 117 dB SPL plus the real-ear-to-coupler difference (RECD) of the 2 cc coupler at the frequency. Methods: The maximum aidable hearing thresholds by hypothetical OTC-compression hearing aids that are compliant with the current FDA guidelines were explored using NAL-NL2 and DSLv5 prescriptive methods. The theoretical maximum permissible overall output levels in the ear canal were also calculated. Additionally, the fitting range of OTC-compression hearing aids with a maximum of 117 dB SPL output level at the ear drum was explored. Results: The current FDA guidelines allow OTC-compression hearing aids to provide sufficient gain and headroom to fit individuals with severe to profound hearing loss. In the presence of a loud wideband signal, the maximum overall output level can reach 164.5 dB SPL in the ear canal for an OTC-compression hearing aid with a bandwidth of 5657 Hz. When the maximum output levels are defined at the ear drum (i.e., the RECD is already considered), however, the fitting range is lowered to moderate hearing loss and the maximum overall output level is lowered by 10 dB. Conclusions: These findings identified potential regulatory vulnerability in the current FDA guidelines and suggest the need to take the measurement coupler and its corresponding RECDs into account when setting the guidelines for OTC hearing aids. Other alternative strategies to prevent potential abuses of the guidelines are also discussed. Full article

Juniperus species are a rich source of pharmacologically important secondary metabolites, including terpenoids, lignans, flavonoids, and phenolic compounds. However, the commercial utilization of these metabolites is limited by the plant’s slow growth, poor natural regeneration, and environmental variability affecting metabolite accumulation. Integrating plant tissue culture with elicitation strategies is a promising approach for sustainably producing these valuable compounds. This review summarizes the diversity, biological activities, and biotechnological significance of secondary metabolites in Juniperus, focusing on tissue culture systems and enhancement of metabolite biosynthesis through elicitors. It critically evaluates the current knowledge of the effects of signaling compounds such as methyl jasmonate (MeJA), jasmonic acid (JA), and salicylic acid (SA), as well as other abiotic and biotic elicitors including silver nanoparticles (AgNPs), chitosan, and chito-oligosaccharides. Particular attention is given to their effects on podophyllotoxin, phenolic, flavonoid, and other bioactive metabolite accumulation. Elicitation responses appear to depend strongly on species, culture system, elicitor type, and treatment conditions. This review also highlights major knowledge gaps, particularly the limited understanding of the regulatory mechanisms controlling secondary-metabolite biosynthesis in Juniperus species. Overall, the available evidence suggests that integrating optimized elicitation strategies with transcriptomics, metabolomics, and metabolic engineering could improve our understanding of secondary metabolite regulation and facilitate the development of sustainable Juniperus tissue culture platforms for producing high-value natural products. Full article

Salinity constrains tropical fruit production, yet the physiological and metabolic responses of Passiflora edulis Sims f. flavicarpa to salt stress and glycine betaine (GB) remain insufficiently characterized. Passion fruit seedlings were evaluated in a completely randomized 2 × 3 factorial design with two GB treatments (0 and 100 mM), three salinity levels (0, 1.6, and 3.2 dS m⁻¹), and five biological replicates per combination. Two-way ANOVA followed by Tukey’s test at 5% was used where applicable. Leaf water potential, SPAD, detached-leaf water loss, and GC–MS-based metabolite profiles were assessed. At 3.2 dS m⁻¹, plants without GB exhibited the most negative leaf water potential, whereas GB maintained a significantly less negative value. SPAD remained relatively stable, while detached-leaf water-loss patterns varied among treatments. Metabolomic profiling revealed changes in sugars, amino acids, and organic acids. Under the higher salinity treatment, GB was associated with higher levels of glucose, sucrose, glycine, proline, citric acid, malic acid, and succinic acid, together with a distinct PCA profile. These findings indicate that GB partially alleviated effects of the higher salinity treatment by improving plant water status and modifying primary metabolism. Full article

Bisphenol A (BPA) is a non-persistent industrial chemical widely used in the production of polycarbonate plastics and epoxy resins. Due to its mass production and versatility, BPA is ubiquitous in environmental matrices, leading to human exposure through ingestion, dermal contact, and inhalation. As a known endocrine-disrupting chemical (EDC) with estrogenic activity, BPA exposure has been associated with reproductive, metabolic, immune, oncogenic, and developmental effects. Mechanistically, BPA is reported to exert its toxic effects via multiple pathways, including alterations in epigenetic microRNA (miRNA) expression. miRNAs are endogenous non-coding RNA molecules that regulate gene expression by targeting mRNAs, thereby influencing a wide range of cellular and metabolic pathways involved in development and disease. Importantly, this review consolidates evidence suggesting that the biological effects of BPA may, in part, be mediated through miRNA-driven epigenetic modifications, affecting numerous downstream proteins and signaling pathways. Altered miRNA expression induced by BPA exposure is implicated in diverse health outcomes, including reproductive dysfunction, oncogenesis, metabolic disorders, and neurodevelopmental abnormalities. Notably, BPA exposure predominantly results in the upregulation of specific miRNAs, such as miR-21 and miR-146a, although tissue-specific and sex-dependent variations are evident. In this review, we provide a comprehensive overview of human, in vivo, and in vitro studies investigating BPA-induced miRNA dysregulation and its associated biological effects. Full article

Ergothioneine (ESH) is one of the most abundant antioxidants in the human body, but its biological functions remain unclear. In this work, we elucidated the detailed mechanism of the deactivation of divalent copper ions Cu(II) by ESH using optical spectroscopy, NMR, and LC-MS. We found that the reduction of Cu(II) ions to Cu(I) by ESH occurs in a bimolecular reaction of Cu^II(ES⁻)₂ complexes, leading to the formation of the disulfide ESSE. Cu(I) ions remain bound in stable complexes with ESH, preventing their reactions with molecular oxygen. Over longer timescales, ESSE decomposes, regenerating reduced ESH and forming the final reaction product, hercynine (EH). The conversion of one ESH molecule to EH causes the reduction of four Cu(II) ions. We determined the rate constants of the bimolecular reaction between Cu^II(ES⁻)₂ complexes to be k₃ = (8 ± 4) × 10⁴ M⁻¹s⁻¹ and of ESSE hydrolysis to be k₄ = (1.7 ± 0.4) × 10⁻⁵ s⁻¹ and also estimated the formation constant K₁₂ ≈ 1.2 × 10¹⁷ M⁻² for the Cu^II(ES⁻)₂ complex. The high efficiency of Cu(II) reduction and the chelation of the resulting Cu(I) ions in a stable complex supports the hypothesis that protecting cells from oxidation by metal ions is likely one of the main functions of ESH. Full article

Drought is one of the major environmental constraints limiting tomato growth and productivity. Identifying regulators that enhance drought tolerance without compromising plant growth is therefore important for tomato production. Homeodomain-leucine zipper (HD-Zip) transcription factors (TFs) play essential roles in plant development and abiotic stress responses; however, the functions of most HD-Zip II members in tomato remain poorly understood. Here, we identified SlHZ07, a drought-responsive HD-Zip II TF, through transcriptome analysis and characterized its biological function in tomato. SlHZ07 was rapidly induced by drought stress and localized predominantly to the nucleus. Overexpression of SlHZ07 significantly enhanced drought tolerance, whereas RNAi-mediated suppression increased drought sensitivity. Physiological analyses showed that SlHZ07 overexpression reduced reactive oxygen species (ROS) accumulation, enhanced antioxidant enzyme activities, upregulated expression of ROS-scavenging genes, and alleviated membrane damage under drought stress. Hormone analyses revealed that SlHZ07 positively regulated jasmonic acid (JA) accumulation and the expression of JA biosynthetic genes, including OPR2, OPR3, JAR1, and AOC, but did not alter endogenous abscisic acid (ABA) levels under well-watered conditions. Furthermore, SlHZ07 promoted vegetative growth by increasing endogenous gibberellin (GA) levels and upregulating the expression of the GA biosynthetic genes GA20ox2 and GA20ox4. Together, our findings identify SlHZ07 as a previously uncharacterized positive regulator that coordinates plant growth and drought adaptation by integrating GA biosynthesis, JA homeostasis, and ROS detoxification. These results expand our understanding of HD-Zip II TFs and provide a promising genetic target for improving drought tolerance in tomato. Full article