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11 pages, 6980 KiB  
Communication
Pollination and Essential Oil Production of Lavandula angustifolia Mill. (Lamiaceae)
by Riley B. Jackson, Tyler M. Wilson, Joseph S. Wilson, Zabrina Ruggles, Lindsey Topham Wilson, Chris Packer, Jacob G. Young, Christopher R. Bowerbank and Richard E. Carlson
Int. J. Plant Biol. 2025, 16(3), 72; https://doi.org/10.3390/ijpb16030072 - 1 Jul 2025
Viewed by 3134
Abstract
Lavandula angustifolia Mill., lavender, is an aromatic plant in the Lamiaceae family. Lavender, which is native to the Mediterranean region but cultivated throughout the world, is an important economic plant. Several studies have investigated two aspects of this aromatic plant: (1) which pollinators, [...] Read more.
Lavandula angustifolia Mill., lavender, is an aromatic plant in the Lamiaceae family. Lavender, which is native to the Mediterranean region but cultivated throughout the world, is an important economic plant. Several studies have investigated two aspects of this aromatic plant: (1) which pollinators, particularly bees, pollinate lavender, and (2) the composition of lavender essential oil. However, little research has been conducted to investigate how pollination affects either the yield or phytochemistry of lavender. The current study, which was conducted in North America, investigates which bee species visit lavender and how pollination affects plant chemistry, specifically the essential oil produced by lavender. Over the course of the 5-week observational period, a total of 12 species (across 10 genera) of bees were identified visiting lavender. Compared to previous studies on cultivated lavender at the same site (Mt. Nebo Botanical Farm, Mona, UT), four bee species not previously observed on lavender were identified. These included Hoplitis producta, Nomada sp., Osmia trevoris, and Megachile snowi. Pollinated lavender, compared to lavender excluded from pollinators, produced more essential oil (yield (w/w) = 1.49% vs. 1.07%), lower relative amounts of linalool (35.4% vs. 39.9%), and higher relative amounts of linalyl acetate (21.3% vs. 16.8%). The findings of this study demonstrate the ecological interactions between pollinators and lavender, and how those interactions impact phytochemistry. Full article
(This article belongs to the Special Issue Plant Resistance to Insects)
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18 pages, 1558 KiB  
Article
The Beekeeping Practice of Transhumance Bee Colonies—Quantitative Study of Honey Production Characteristics Based on a Questionnaire Survey in Hungary
by Aliz Feketéné Ferenczi, Andrea Bauerné Gáthy, István Szűcs and Angéla Kovácsné Soltész
Agriculture 2025, 15(13), 1405; https://doi.org/10.3390/agriculture15131405 - 29 Jun 2025
Viewed by 416
Abstract
This study examined the transhumance of bee colonies from the perspective of Hungarian beekeepers. Hungarian scientific literature on this topic is minimal. Therefore, it is necessary to assess the sustainability of the Hungarian beekeeping sector using a research method based on a survey [...] Read more.
This study examined the transhumance of bee colonies from the perspective of Hungarian beekeepers. Hungarian scientific literature on this topic is minimal. Therefore, it is necessary to assess the sustainability of the Hungarian beekeeping sector using a research method based on a survey of beekeepers. This research was based on a questionnaire (N = 1067). Basic statistical tools were used to analyse the data. To evaluate the differences between the individual category variables, we used the chi-square test for cross-stability and non-parametric tests were used. In this study, we defined four farm (apiary) size categories based on the number of bee colonies. Our results revealed statistically significant correlations between apiary size and transhumance, payment for the use of bee pastures and transhumance, as well as between the type of hive used and transhumance. Transhumance is mainly characteristic of semi-professional and professional apiaries. Hungarian beekeepers (typically small-scale and professional apiary size) traditionally give honey as a “gift” to landowners for the use of bee pastures, which is related to transhumance. In terms of the type of hive used, a significant difference can be seen between transhumance (28 kg/colony) and stationary apiaries (21 kg/colony) in the case of vertical hives. Full article
(This article belongs to the Special Issue Productivity and Efficiency of Agricultural and Livestock Systems)
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15 pages, 338 KiB  
Article
How the Functional Constitution of Plant Communities in Field Margins Affects Wild Bee Community Composition and Functional Structure
by Jane Morrison, Jordi Izquierdo, Eva Hernández Plaza and José L. González-Andújar
Agronomy 2025, 15(6), 1354; https://doi.org/10.3390/agronomy15061354 - 31 May 2025
Viewed by 429
Abstract
Concerns about a global decline in pollinators have called for more knowledge about the drivers of wild pollinator abundance and diversity in agroecosystems. Maintaining flowering plants in agricultural field margins is often recommended as a cost-effective and efficient method of offering habitat for [...] Read more.
Concerns about a global decline in pollinators have called for more knowledge about the drivers of wild pollinator abundance and diversity in agroecosystems. Maintaining flowering plants in agricultural field margins is often recommended as a cost-effective and efficient method of offering habitat for wild pollinator conservation. This research involved a three-year, multi-farm study, examining Mediterranean cereal field margins in order to investigate which general and functional characteristics of margin plant communities were important for sustaining wild bee abundance, diversity, community evenness and functional diversity. Wild bees were collected and identified to genus, and a database was compiled listing the morpho-physiological features and behaviours of the observed genera. A database was also compiled of the flowering plant species observed and relevant trait values. General and generalized linear models indicated that margins with a higher percentage of trees and shrubs and higher floral richness displayed positive effects on wild bee diversity and visits to flowers in Mediterranean cereal agroecosystems. They also indicated that high plant functional diversity, in terms of flower colour and morphology, as well as high nectar accessibility, were important to encourage bee visits and community evenness within wild bee assemblages in these field margins. This study stresses the importance of maintaining protected field margins and, when necessary to restore their functionality, sowing floral mixtures with diverse native species, including trees and shrubs, and providing plenty of accessible nectar and a diverse assortment of colours and shapes. Full article
(This article belongs to the Section Farming Sustainability)
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5 pages, 192 KiB  
Proceeding Paper
Honey Cost: An Experimental Approach for Determining the Production Costs of Honey
by Concetta Cardillo, Antonio Giampaolo and Milena Verrascina
Proceedings 2025, 117(1), 2; https://doi.org/10.3390/proceedings2025117002 - 17 Apr 2025
Viewed by 520
Abstract
The Honey Cost project, conceived and developed by the CREA Policies and Bioeconomics research center (CREA PB), will develop a methodology and tools for ad hoc data collection and evaluation of the bee and honey sector and sets up a sample survey on [...] Read more.
The Honey Cost project, conceived and developed by the CREA Policies and Bioeconomics research center (CREA PB), will develop a methodology and tools for ad hoc data collection and evaluation of the bee and honey sector and sets up a sample survey on it. The determination of the cost of honey production is carried out through a questionnaire, submitted to a sample of 434 farms with an economic size of at least EUR 8,000 of Standard Output. In the methodology adopted, three cost levels are envisaged, with the first level referring only to current expenses, the second level adding other general expenses, and, finally, the third level also adding the cost of family labor. The first survey, carried out in 2023, covered the two-year period of 2021–2022. It emerged that among the variable costs, those incurred for packaging and marketing represent, in percentage, the most important item, followed by nutrition costs, and their incidence varies depending on the farm type and on the economic size. Even among the components of general costs (depreciation and other non-direct expenses), a strong variability was found, and this attenuates when moving from small to large farms. The total production cost stands, as a sample average, at around EUR 9 per kilogram of honey produced. For many farms, this value is well above the price that is recognized by the market for their product. Full article
19 pages, 3161 KiB  
Article
Antibiotic Use in Beekeeping: Implications for Health and Environment from a One-Health Perspective
by Michela Mosca, Andrea Gyorffy, Marcella Milito, Camilla Di Ruggiero, Alessandra De Carolis, Marco Pietropaoli, Luigi Giannetti, Francesco Necci, Francesca Marini, Daniele Smedile, Manuela Iurescia, Alessia Franco, Antonio Battisti, Pasquale Rombolà, Marcella Guarducci and Giovanni Formato
Antibiotics 2025, 14(4), 359; https://doi.org/10.3390/antibiotics14040359 - 1 Apr 2025
Viewed by 923
Abstract
Background: The use of antibiotics in beekeeping has potential implications for honeybee health and environmental contamination. Recent research indicates that extensive antibiotic use in beekeeping, especially oxytetracycline, promotes antimicrobial resistance in bee-related bacteria. Honeybees can transport oxytetracycline-resistance genes during foraging, potentially establishing reservoirs [...] Read more.
Background: The use of antibiotics in beekeeping has potential implications for honeybee health and environmental contamination. Recent research indicates that extensive antibiotic use in beekeeping, especially oxytetracycline, promotes antimicrobial resistance in bee-related bacteria. Honeybees can transport oxytetracycline-resistance genes during foraging, potentially establishing reservoirs of resistance in the colony and facilitating intergeneric gene transfer among various gut bacteria as well as in the microbiome of the flowers and the wider environment, where honeybees can spread antibiotic-resistance genes over a large distance. This study investigates the effects of oxytetracycline hydrochloride (OTC) treatment on honeybees from a One Health perspective, examining antibiotic residues in honey, environmental spread, and the presence of tetracycline-resistance genes (TET-RGs). Methods: In the spring of 2022, two groups of four honeybee hives were placed near an almond grove in Central Italy. One group was treated with 1.68 g of OTC, while the other remained untreated. Samples were collected from bees, honey, hive entrances, and flowers before treatment and at 3 as well as 9 days post-treatment. OTC residues and TET-RGs were analyzed to assess contamination and resistance gene dissemination. Results: OTC residues were detected in honey from both treated (day 3: 263,250.0 ± 100,854.3 µg/kg; day 9: 132,600 ± 146,753.9 µg/kg) and untreated hives (day 3: 20.5 ± 8.2 µg/kg; day 9: 135.8 ± 198.6 µg/kg), suggesting cross-contamination. Residues were also found in almond tree flowers (0.7 ± 0.1 µg/kg), with TET-RGs (tet(K), tet(L), tet(M), tet(B), tet(O), tet(D)) detected pre- and post-treatment. In honeybee gut bacteria, resistance genes (tet(M), tet(A), tet(D), tet(B)) appeared post-treatment in both groups. No significant correlation was observed between hive distance and resistance gene presence in flowers, although the presence of other farms located within the bees’ flight range, in which OTC might have been used in the past, could have influenced the results. Conclusions: These findings highlight the risk of OTC-induced antibiotic cross-contamination and the spread of TET-RG, raising concerns for bee health and environmental safety. Given honeybees’ social nature and the negative effects of antibiotics on their health, an antibiotic-free management approach is recommended for sustainable apiculture. Full article
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10 pages, 1125 KiB  
Article
Comparison of Gut Microbiota in Overwintering Bees: Apis cerana vs. Apis mellifera
by Heng Chen, Lijiao Gao, Jialin Liu, Conghui Ji, Xiaoqun Dang, Zeyang Zhou and Wenhua Luo
Microbiol. Res. 2024, 15(4), 2425-2434; https://doi.org/10.3390/microbiolres15040163 - 26 Nov 2024
Cited by 1 | Viewed by 1350
Abstract
Bees play important roles in socio-economic development, biodiversity conservation, and ecosystem stability. However, during the cold season, resources become limited, leading to significant losses in bee colonies. Although many studies have described the characteristics of winter bees and demonstrated that notable changes occur [...] Read more.
Bees play important roles in socio-economic development, biodiversity conservation, and ecosystem stability. However, during the cold season, resources become limited, leading to significant losses in bee colonies. Although many studies have described the characteristics of winter bees and demonstrated that notable changes occur in their gut microflora, the underlying mechanisms remain yet to be fully elucidated. Therefore, this study was conducted to compare the gut microbiota dynamics of overwintering bees. Sample acquisition involved randomly selecting ten colonies each from three bee farms containing Apis cerana (AC) and Apis mellifera (AM), followed by dissection for further analysis. DNA was extracted, and 16S rDNA sequencing, along with various bioinformatics tools, was used to assess microbial diversity, functional differences, and species comparisons between AC and AM gut microbiota. AC exhibited lower β diversity in the gut microbiota than AM during winter. Moreover, Gilliamella and Apibacter were relatively more abundant in AC. Regarding microbial functions, key pathways included the phosphotransferase system, galactose metabolism, the pentose phosphate pathway, and carbohydrate transport and metabolism. These results suggest the presence of microbial diversity differences between AC and AM, with the differential microbial functions mainly enriched in metabolic pathways that facilitate adaptation to cold environmental stress. Full article
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29 pages, 3414 KiB  
Article
The Calibrated Safety Constraints Optimal Power Flow for the Operation of Wind-Integrated Power Systems
by Kai-Hung Lu, Wenjun Qian, Yuesong Jiang and Yi-Shun Zhong
Processes 2024, 12(10), 2272; https://doi.org/10.3390/pr12102272 - 17 Oct 2024
Cited by 1 | Viewed by 897
Abstract
As the penetration of renewable energy sources (RESs), particularly wind power, continues to rise, the uncertainty in power systems increases. This challenges traditional optimal power flow (OPF) methods. This paper proposes a Calibrated Safety Constraints Optimal Power Flow (CSCOPF) model that uses the [...] Read more.
As the penetration of renewable energy sources (RESs), particularly wind power, continues to rise, the uncertainty in power systems increases. This challenges traditional optimal power flow (OPF) methods. This paper proposes a Calibrated Safety Constraints Optimal Power Flow (CSCOPF) model that uses the Improved Acceleration Coefficient-Based Bee Swarm algorithm (IACBS) in combination with the equivalent current injection (ECI) model. The proposed method addresses key challenges in wind-integrated power systems by ensuring preventive safety scheduling and enabling effective power incident safety analysis (PISA). This improves system reliability and stability. This method incorporates mixed-integer programming, with continuous and discrete variables representing power outputs and control mechanisms. Detailed numerical simulations were conducted on the IEEE 30-bus test system, and the feasibility of the proposed method was further validated on the IEEE 118-bus test system. The results show that the IACBS algorithm outperforms the existing methods in both computational efficiency and robustness. It achieves lower generation costs and faster convergence times. Additionally, the CSCOPF model effectively prevents power grid disruptions during critical incidents, ensuring that wind farms remain operational within predefined safety limits, even in fault scenarios. These findings suggest that the CSCOPF model provides a reliable solution for optimizing power flow in renewable energy-integrated systems, significantly contributing to grid stability and operational safety. Full article
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16 pages, 10032 KiB  
Article
Foraging of Honeybees from Different Ecological Areas Determined through Melissopalynological Analysis and DNA Metabarcoding
by Saule Daugaliyeva, Aida Daugaliyeva, Katira Amirova, Akmeiir Yelubayeva, Abylay Kapar, Aishat Abiti, Thomas Partipilo, Nurlan Toktarov and Simone Peletto
Insects 2024, 15(9), 674; https://doi.org/10.3390/insects15090674 - 5 Sep 2024
Viewed by 2383
Abstract
The environment significantly impacts the lives of bees and their feeding. This study aimed to investigate bee foraging using melissopalynological analysis and DNA metabarcoding in intensive farming, reserved, and urbanized areas. The highest alpha diversity was observed in the reserved and intensive farming [...] Read more.
The environment significantly impacts the lives of bees and their feeding. This study aimed to investigate bee foraging using melissopalynological analysis and DNA metabarcoding in intensive farming, reserved, and urbanized areas. The highest alpha diversity was observed in the reserved and intensive farming areas. The urbanized area had less diversity. In the intensive farming area, Sinapis, Helianthus, and Fagopyrum predominated; in the reserved area, Melilotus, Helianthus, and Brassica predominated. In the urbanized area, garden plants, namely radish (Raphanus sativus) and cucumber (Cucumis hystrix), and agricultural plants, namely soybean (Glycine max) and melon (Cucumis melo), were often found. The most significant agreement was between the rbcL and the melissopalynological analysis. The ITS2 revealed equal matches with both rbcL and melissopalynology, but this marker missed or underestimated some genera. Trifolium pretense and Brassica nigra were identified simultaneously by the melissopalinology method and two genetic markers in DNA metabarcoding. The species Convolvulus arvensis, Melilotus officinalis, Echium vulgare, Brassica rapa, Helianthus divaricatus, and Onobrychis viciifolia were found in all ecological areas. Imperfect databases impose some limits in the identification of some taxa using metabarcoding. The further research and expansion of plant databases is needed. Studying the food preferences of bees in different environmental conditions and landscapes is necessary to develop measures to preserve their populations. Full article
(This article belongs to the Section Insect Ecology, Diversity and Conservation)
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17 pages, 11599 KiB  
Article
Optimization of Fuzzy Control Parameters for Wind Farms and Battery Energy Storage Systems Based on an Enhanced Artificial Bee Colony Algorithm under Multi-Source Sensor Data
by Zejian Liu, Ping Yang, Peng Zhang, Xu Lin, Jiaxi Wei and Ning Li
Sensors 2024, 24(16), 5115; https://doi.org/10.3390/s24165115 - 7 Aug 2024
Cited by 2 | Viewed by 1230
Abstract
With the rapid development of sensors and other devices, precise control for the generation of new energy, especially in the context of highly stochastic wind power generation, has been strongly supported. However, large-scale wind farm grid connection can cause the power system to [...] Read more.
With the rapid development of sensors and other devices, precise control for the generation of new energy, especially in the context of highly stochastic wind power generation, has been strongly supported. However, large-scale wind farm grid connection can cause the power system to enter a low inertia state, leading to frequency instability. Battery energy storage systems (BESSs) have the advantages of a fast response speed and high flexibility, and can be applied to wind farm systems to improve the frequency fluctuation problem in the process of grid connection. To address the frequency fluctuation problem caused by the parameter error of the fuzzy membership function in the fuzzy control of a doubly fed induction generator (DFIG) and a BESS, this paper proposes an improved Artificial Bee Colony (ABC) algorithm based on multi-source sensor data for optimizing the fuzzy controller to improve the frequency control ability of BESSs and DFIGs. A Gaussian wandering mechanism was introduced to improve the ABC algorithm and enhance the convergence speed of the algorithm, and the improved ABC algorithm was optimized for the selection of fuzzy control affiliation function parameters to improve the frequency response performance. The effectiveness of the proposed control strategy was verified on the MATLAB/Simulink simulation platform. After optimization using the proposed control strategy, the oscillation amplitude was reduced by 0.15 Hz, the precision was increased by 40%, and the steady-state frequency deviation was reduced by 26%. The results show that the method proposed in this paper provides a great improvement in the frequency stability of coordinated systems of wind farms and BESSs. Full article
(This article belongs to the Section Sensor Networks)
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15 pages, 2160 KiB  
Review
Review: Nutritional Needs of Honeybees and Legislation on Apiculture By-Products in Animal Nutrition
by Patrick Gernt, Julia Dittes, Ingrid Vervuert and Ilka U. Emmerich
Animals 2024, 14(15), 2208; https://doi.org/10.3390/ani14152208 - 30 Jul 2024
Cited by 1 | Viewed by 2073
Abstract
Honeybees are some of the smallest farmed animals, and apiculture by-products, e.g., honey, beeswax, propolis, royal jelly, and pollen contribute to animal nutrition. For the effective production of these by-products, the optimal development and nutrient supply of the honeybee is required. Beginning with [...] Read more.
Honeybees are some of the smallest farmed animals, and apiculture by-products, e.g., honey, beeswax, propolis, royal jelly, and pollen contribute to animal nutrition. For the effective production of these by-products, the optimal development and nutrient supply of the honeybee is required. Beginning with the development of the mouth and anal pores on the second day of embryonic development, the digestive tract differentiates into the mouth and fore-, mid-, and hindgut during the pupal stage. The various glands within the oral cavity are particularly important, secreting enzymes and substances that are crucial for digestion and hive nutrition, e.g., invertase and royal jelly. Honeybees rely on a specialized caste system, with worker bees collecting nectar, pollen, water, and resin for the nutrition of the entire hive. Macronutrients, including proteins, carbohydrates, and lipids, obtained primarily from pollen and nectar, are essential for the growth and development of larvae and the overall health of the colony. Inadequate nutrient intake can lead to detrimental effects on larval development, prompting cannibalism within the hive. Apiculture by-products possess unique nutritional and therapeutic properties, leading to a growing interest in the use of honey, beeswax, propolis, and pollen as a feed additive. In recent years, the use of apicultural by-products in animal nutrition has been primarily limited to in vivo studies, which have demonstrated various positive impacts on the performance of farm animals. Honey, beeswax, propolis, royal jelly, and pollen are listed feed stuffs according to Regulation (EC) No. 68/2013. However, for animal nutrition there is not any specific legal definition for these products and no legal requirements regarding their ingredients as given for honey or beeswax in European food law. Full article
(This article belongs to the Section Public Policy, Politics and Law)
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13 pages, 2695 KiB  
Communication
Toxic and Environmental Effects of Neonicotinoid Based Insecticides
by Zarook Shareefdeen and Ali Elkamel
Appl. Sci. 2024, 14(8), 3310; https://doi.org/10.3390/app14083310 - 15 Apr 2024
Cited by 4 | Viewed by 4082
Abstract
The insecticide known as neonicotinoid has negative impacts on the ecosystem, human health, and the environment; specifically, its effects on the relationship between crop yields and the death rate of natural pollinators, such as bees, affect food security. The active ingredients in neonicotinoids [...] Read more.
The insecticide known as neonicotinoid has negative impacts on the ecosystem, human health, and the environment; specifically, its effects on the relationship between crop yields and the death rate of natural pollinators, such as bees, affect food security. The active ingredients in neonicotinoids include imidacloprid, clothianidin, thiamethoxam, acetamiprid, sulfoxaflor, and thiacloprid, which are sold under various trade names. For many of the components of these toxic insecticides, patents have been expired; however, farmers and consumers who continue to use these chemicals are unaware of the products’ toxicity and the environmental effects they have. Thus, agricultural industries are required to consider diverse methods to minimize neonicotinoid use in farming operations and move away from the current prevailing methods. In this short review, the negative effects of neonicotinoid use; the toxic components, health effects, and environmental regulations of neonicotinoids; and sustainable methods to minimize their use are examined. Full article
(This article belongs to the Section Environmental Sciences)
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15 pages, 3357 KiB  
Article
The Bee Communities of Young Living Lavender Farm, Mona, Utah, USA
by Joseph S. Wilson, Jacob G. Young and Lindsey Topham Wilson
Diversity 2024, 16(2), 119; https://doi.org/10.3390/d16020119 - 13 Feb 2024
Cited by 2 | Viewed by 9421
Abstract
It is now widely recognized that bees are among the most important pollinators worldwide, yet the bee faunas of many regions and habitats remain inadequately documented. The Great Basin Desert in North America is thought to host some of the richest bee communities [...] Read more.
It is now widely recognized that bees are among the most important pollinators worldwide, yet the bee faunas of many regions and habitats remain inadequately documented. The Great Basin Desert in North America is thought to host some of the richest bee communities in the world, as indicated by several studies documenting diverse bee faunas in the region’s natural habitats. However, limited attention has been given to the bee communities present on agricultural lands within the Great Basin Desert. Here, we describe a rich bee community housed at the Young Living Lavender Farm in Juab County, Utah, near the eastern edge of the Great Basin Desert. Our survey of bees on this farm identified 68 bee species across 22 genera. This represents 34% of the bee species known from the county, including 34 new county records. Among the numerous flower species cultivated at the farm, we found that lavender supported the richest bee community, with 32 species collected from cultivated lavender fields. While lavender is frequently recommended for homeowners to plant in support of pollinators, our study is among the first to provide a list of bee species that visit lavender in western North America. Furthermore, our results demonstrate that agricultural lands, particularly those implementing pollinator-friendly farming practices, can support rich bee communities in the Great Basin Desert. Full article
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20 pages, 4133 KiB  
Article
Designing Management Strategies for Sheep Production and Bees in Dryland Pastures
by Mia Caudillo, Andony Melathopoulos, David Eduardo Prado-Tarango, Mary Smallman, Sarah A. Taylor and Serkan Ates
Agronomy 2024, 14(1), 24; https://doi.org/10.3390/agronomy14010024 - 21 Dec 2023
Cited by 4 | Viewed by 1563
Abstract
Novel grazing management practices for livestock and bee health are becoming increasingly crucial in pasture-based farming systems. The effect of pasture type and spring closing dates on lamb liveweight gain, pasture production, botanical composition, bloom density and bee visitation was monitored over 2 [...] Read more.
Novel grazing management practices for livestock and bee health are becoming increasingly crucial in pasture-based farming systems. The effect of pasture type and spring closing dates on lamb liveweight gain, pasture production, botanical composition, bloom density and bee visitation was monitored over 2 years. Total annual dry matter yield (DMY) of diverse pastures in 2020/2021 was 8.8 t DM ha−1. This yield was greater than the DMY obtained from both simple (7.6 t DM ha−1) and legume pastures (6.6 t DM ha−1). In 2021/2022, the total annual DMY of simple (8.6 t DM ha−1) and diverse pastures (9.0 t DM ha−1) was similar. However, the legume pastures produced 27–30% less than simple and diverse pastures. In successive years, lambs grew faster in legume pastures (287, 215 g per head d−1) than diverse (207, 151 g per head d−1) and simple pastures (204, 132 g per head d−1). However, spring liveweight production (kg ha−1 day−1) from pastures did not differ due to the lower stocking density of legume pastures as compared to the other two pasture mixtures. Bloom density (flower/m2) and bee visitation (bees/min−1 m2) were 16 and 40 times greater with legume rather than simple pastures. Bloom density for diverse pastures was also relatively lower than for the legume pastures. Our findings indicated that the diversification of pastures greatly increased pasture productivity, while legume pastures provided the highest bee benefit without penalizing lamb liveweight production in spring. Full article
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16 pages, 2435 KiB  
Article
Chemical Profile of Essential Oils of Selected Lamiaceae Plants and In Vitro Activity for Varroosis Control in Honeybees (Apis mellifera)
by Roberto Bava, Fabio Castagna, Carmine Lupia, Stefano Ruga, Vincenzo Musella, Filomena Conforti, Mariangela Marrelli, Maria Pia Argentieri, Domenico Britti, Giancarlo Statti and Ernesto Palma
Vet. Sci. 2023, 10(12), 701; https://doi.org/10.3390/vetsci10120701 - 13 Dec 2023
Cited by 4 | Viewed by 2548
Abstract
The most significant ectoparasitic mite of honeybees, Varroa destructor, has a detrimental effect on bee health and honey output. The principal strategy used by the control programs is the application of synthetic acaricides. All of this has resulted in drug resistance, which [...] Read more.
The most significant ectoparasitic mite of honeybees, Varroa destructor, has a detrimental effect on bee health and honey output. The principal strategy used by the control programs is the application of synthetic acaricides. All of this has resulted in drug resistance, which is now a major worry for beekeeping. As a result, research on alternate products and techniques for mite management is now required. The aim of this study was to determine whether essential oils (EOs) extracted from botanical species of Lamiacae, typical of the Calabria region of Southern Italy, could reduce the population of the mite V. destructor. Among the best-known genera of the Lamiaceae family are oregano, rosemary and thyme, whose EOs were employed in this study. By steam distillation, the EOs were extracted from Origanum vulgare subsp. viridulum (Martrin-Donos) Nyman, Thymus capitatus Hoffmanns. and Link, Thymus longicaulis C.Presl and Salvia rosmarinus Schleid. plant species harvested directly on the Calabrian territory in their balsamic time. Each EO went to the test in vitro (contact toxicity) against V. destructor. Fifty adult female mites, five for each EO and the positive and negative control, were used in each experimental replicate. The positive controls comprised five individuals treated to Amitraz dilute in acetone, and the negative controls included five individuals exposed to acetone alone. To create the working solution to be tested (50 μL/tube), the EOs were diluted (0.5 mg/mL, 1 mg/mL, 2 mg/mL and 4 mg/mL) in HPLC-grade acetone. After 1 h of exposure, mite mortality was manually assessed. Origanum vulgare subsp. viridulum, Thymus capitatus and Thymus longicaulis were the EOs with the highest levels of efficiency at 2 mg/mL, neutralizing (dead + inactivated), 94%, 92% and 94% of parasites, respectively. Salvia rosmarinus EO gave a lower efficacy, resulting in a percentage of 38%. Interestingly, no adverse effects were highlighted in toxicity tests on honeybees. These results show that these OEs of the Lamiaceae family have antiparasitic action on V. destructor. Therefore, they could be used, individually or combined, to exploit the synergistic effect for a more sustainable control of this parasite mite in honeybee farms. Full article
(This article belongs to the Special Issue Pharmacokinetics and Pharmacodynamics in Animal Clinical Treatment)
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12 pages, 1332 KiB  
Article
Occurrence of Glyphosate and Other Polar Pesticides in Honey from Lombardy and Emilia-Romagna Regions in Italy: Three-Year Monitoring Results
by Elena Butovskaya, Mara Gasparini, Barbara Angelone, Gabriella Cancemi, Vito Tranquillo, Giovanni Prestini, Filippo Bosi and Simonetta Menotta
Foods 2023, 12(24), 4448; https://doi.org/10.3390/foods12244448 - 12 Dec 2023
Cited by 5 | Viewed by 1909
Abstract
Intensive agricultural practices, such as pesticides use, may negatively affect bee health and hive products. Glyphosate is one of the most widely used polar pesticides applied in crops for weed control. In this study, honey samples, collected from beekeeping farms located in the [...] Read more.
Intensive agricultural practices, such as pesticides use, may negatively affect bee health and hive products. Glyphosate is one of the most widely used polar pesticides applied in crops for weed control. In this study, honey samples, collected from beekeeping farms located in the Lombardy and Emilia-Romagna regions in Italy in the framework of regional monitoring plans activated from 2020 to 2022, were analyzed for the presence of residues of polar pesticides. The analytical method based on ion chromatography coupled to high-resolution mass spectrometry was applied to quantify glyphosate, glufosinate, ethephon, fosetyl aluminum, and their related metabolites. Residues of glyphosate were detected in around 28% of analyzed honey samples. Observations on the distribution of the honey-production-site locations suggest that honey samples originating from the provinces within the Lombardy region, where the agricultural sector is highly developed, were more affected by glyphosate contamination than the samples collected from the areas with low agricultural activity, where no glyphosate residues were detected over the three years of the monitoring program. Full article
(This article belongs to the Special Issue Analysis of the Pesticide Residues and/or Mycotoxins in Foods)
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