Search Results (1,450)

Background: Skin photoaging, primarily induced by chronic ultraviolet (UV) radiation exposure, is characterized by dryness, wrinkle formation, pigmentation abnormalities, and reduced skin elasticity, resulting from oxidative stress, inflammation, and degradation of the extracellular matrix. Codonopsis pilosula, a traditional food–medicine homologous plant, is recognized for its anti-aging properties. However, its lipophilic components (designated as CP-L) remain insufficiently explored. Methods: Herein, we developed a thermosensitive nanogel encapsulating CP-L-loaded transferosomes (CP-L nanogel) to enhance topical delivery and evaluated its effects in both a UV-induced photoaging mouse model and UVB-irradiated HaCaT keratinocytes. Results: In UV-induced mice, topical application of the nanogel markedly reduced skin wrinkling and epidermal hyperplasia, with epidermal thickness decreased by 83.2% compared to the model group (p < 0.01), and restored skin elasticity and collagen deposition, as evidenced by a 35.5% increase in collagen area fraction (p < 0.01). Correspondingly, in UVB-irradiated HaCaT cells, it significantly increased cell viability from 53.0 ± 9.6% to 89.4 ± 1.0% (p < 0.01) and suppressed apoptosis from 30.1 ± 0.48% to 12.4 ± 0.66% (p < 0.01). Furthermore, the CP-L nanogel consistently attenuated oxidative stress, with SOD, CAT, and GSH-Px activities increased by 73.1%, 188.1%, and 18.2%, respectively (p < 0.01), and MDA levels reduced by 71.0% (p < 0.01), while inflammatory responses were suppressed, as TNF-α, IL-1α, IL-1β, and IL-6 levels decreased by 28.3%, 22.6%, 12.8% and 31.9%, respectively (p < 0.01). Mechanistically, transcriptomic and molecular analyses revealed that the nanogel potently inhibited the UV-induced activation of the FGFR/PI3K/AKT/mTOR/p70S6K signaling cascade at both transcriptional and protein levels, with the phosphorylation levels of FGFR, PI3K, AKT, mTOR, and p70S6K significantly reduced by 43.9%, 30.9%, 38.8%, 34.9%, and 57.3%, respectively (p < 0.01). Molecular docking and dynamics simulations identified isofuranodienone and aromadendrene oxide-(2) as key constituents with high-affinity, stable binding to FGFR1 and AKT1. The cytoprotective effect of the nanogel was completely abolished by co-treatment with the FGFR inhibitor PD173074, confirming functional reliance on this pathway. Enhanced cellular delivery of the formulation was directly demonstrated by flow cytometry, showing an approximately 1.8-fold increase in cellular uptake compared to the free drug (p < 0.01). Conclusions: Collectively, these results demonstrated that the CP-L nanogel alleviated skin photoaging through a multi-faceted mechanism involving enhanced cellular delivery, potent antioxidant and anti-inflammatory activities, and specific inhibition of the FGFR/PI3K/AKT/mTOR signaling cascade, highlighting its potential as a multitargeted topical agent derived from an edible plant. Full article

Fungal-derived compounds are attracting increasing interest in cosmetic science because of their chemical diversity, multifunctional biological activity, and suitability for controlled biotechnological production. Macrofungi produce a wide range of bioactive metabolites, including polysaccharides, phenolic compounds, terpenoids, sterols, and pigments, many of which exhibit antioxidant, anti-inflammatory, moisturizing, photoprotective, and barrier-supporting properties relevant to skin health. However, current research is largely limited to isolated metabolites and simplified in vitro models, whereas comparatively little attention has been paid to the performance of fungal-derived compounds in finished cosmetic formulations. In practice, cosmetic efficacy depends not only on biological activity but also on extraction methods, physicochemical stability, formulation compatibility, and delivery efficiency. This review examines fungal-derived bioactive compounds from the perspective of cosmetic formulation and industrial applicability. It discusses how cultivation conditions, extraction procedures, and formulation design influence stability, bioavailability, and cosmetic performance, and critically evaluates extraction technologies, fermentation systems, encapsulation strategies, lipid carriers, hydrogels, and nanoscale delivery platforms. The review also addresses major limitations, including oxidative instability, variability in metabolite composition, limited penetration of high-molecular-weight compounds, and insufficient standardization and clinical validation. Although fungal-derived compounds show considerable promise for multifunctional cosmetic formulations, their broader industrial application will require improved standardization, optimized formulation strategies, and robust clinical evaluation of efficacy and safety. Full article

The olive oil industry generates large amounts of by-products, particularly olive pomace and olive mill wastewater, which represent valuable sources of polyphenolic compounds with well-recognized antioxidant, anti-inflammatory, antimicrobial, and health-promoting properties. Within the framework of the circular economy, the valorization of these olive-derived residues has emerged as a promising strategy for converting low-value waste into high-added-value ingredients for food, pharmaceutical, and cosmetic applications. This review provides a comprehensive overview of recent advances in sustainable extraction and sample preparation approaches for the recovery of olive polyphenols, including ultrasound-, microwave-, enzyme-, and membrane-assisted technologies, as well as the use of green solvents and environmentally friendly extraction systems. Furthermore, the review discusses advanced analytical strategies for the identification and quantification of olive-derived polyphenols, covering chromatographic, spectrometric, electrophoretic, and miniaturized analytical platforms. Special attention is given to biopolymer-based encapsulation systems developed to enhance stability, controlled release, bioaccessibility, and bioavailability of these bioactive compounds. Finally, current challenges and future perspectives regarding the integration of green extraction technologies, analytical methodologies, and advanced delivery systems are highlighted to promote the sustainable exploitation of olive by-products within a circular economy framework. Full article

Xanthohumol (XN), a prenylated chalcone isolated from Humulus lupulus L., exhibits a wide range of biological activities, including antioxidant, anti-inflammatory, and chemopreventive effects. However, its therapeutic application is limited by poor aqueous solubility, low chemical stability, and rapid clearance from the systemic circulation. The present study aimed to develop and characterize a novel nano-sized drug-delivery system for XN that combines favourable colloidal stability, efficient encapsulation, sustained release, and reduced recognition by macrophages (“stealth-like” properties). To achieve this, XN-loaded cationic liposomes were coated with two marine polysaccharides, iota-carrageenan (CAR) and fucoidan (FUC), followed by Ca²⁺-mediated cross-linking. Liposomes were prepared by the ethanol injection method, and formulation parameters were optimized using a 2³ + 1 full factorial design. Surface modification and cross-linking conditions were further optimized through polyelectrolyte titration and a Taguchi L9 orthogonal array. The resulting nanocarriers were evaluated for particle size, polydispersity, ζ-potential, encapsulation efficiency, release behavior, and cellular uptake. Both coatings significantly prolonged XN release compared with uncoated liposomes, with CAR-coated vesicles providing the most sustained release (≈55% over 48 h). In RAW264.7 macrophages, 50 µg/mL CAR-coated liposomes reduced cellular uptake by approximately 74% following 1-h incubation relative to uncoated controls and maintained this reduction over 2 h whereas FUC-coated vesicles afforded only transient early evasion. The cross-linked iota-carrageenan coating thus represents a promising strategy for conferring stable “stealth-like” characteristics to XN-loaded liposomes intended for prolonged drug delivery. Full article

This study investigated the potential of cellulose as a carrier for bioactive phenolic compounds from tart cherry juice. Using a freeze-drying method, encapsulates were prepared by varying cellulose content (2.5%, 5%, 7.5%, and 10%) and complexation times (15 and 60 min) while maintaining a constant juice volume. The prepared encapsulates were characterized by determining the concentrations of total phenols, monomeric anthocyanins, and proanthocyanidins, alongside antioxidant activity by spectrophotometric methods, individual polyphenols by HPLC, color parameters and structural changes via IR spectroscopy. Additionally, the stability of these parameters was evaluated after one year of storage at room temperature. Results indicated that the highest concentrations of phenolic compounds and the strongest antioxidant potential were achieved with 2.5% cellulose content (the lowest content) after 15 min of complexation (shorter time). Concentrations of polyphenols decreased as the cellulose contents increased. Although changes in chemical parameters occurred during storage, the results confirm that cellulose is a viable carrier for tart cherry phenolics, particularly at lower contents, offering a promising approach for formulating stable bioactive delivery systems. Full article

Kombucha is a fermented tea beverage and a rich source of bioactive compounds with potential health benefits. This study aimed to evaluate the effects of different tea substrates (green, oolong, and black tea) on fermentation characteristics, bioactive compound profiles, antioxidant activity, and cytotoxicity, and to develop a nanoemulsion system for the encapsulation of kombucha-derived bioactive compounds for food applications. Kombucha was fermented for 15 days and during microbial growth, pH, total acidity, and sugar consumption were monitored. The results showed that green tea kombucha exhibited the lowest pH and highest total acidity, indicating more active fermentation. High-performance liquid chromatography analysis revealed that concentrated green tea kombucha contained higher levels of catechin, caffeine, and selected organic acids, whereas black tea kombucha exhibited the highest epigallocatechin gallate (EGCG) concentration. Among the tested samples, green tea kombucha demonstrated superior antioxidant properties as determined by DPPH, ABTS, and FRAP assays, while exhibiting no significant cytotoxicity in RAW 264.7, Caco-2, and A549 cells. Based on these findings, a nanoemulsion system was developed using concentrated green tea kombucha. The nanoemulsion exhibited an average particle size of 110.03 ± 6.2 nm, a polydispersity index of 0.28 ± 0.01, and a zeta potential of +32.5 ± 0.5 mV. Furthermore, the nanoemulsion maintained acceptable physicochemical stability during 6 months of storage at 4 °C, remaining within the nanoscale range with only minor changes in PDI and zeta potential. The encapsulation efficiency reached 91.66 ± 2.29%, and the system demonstrated a biphasic release profile with an initial burst followed by sustained release. Cellular uptake studies confirmed efficient internalization of the nanoemulsion in all tested cell lines. These findings highlight the potential of green tea kombucha as a functional food ingredient and demonstrate the feasibility of nanoemulsion-based systems for the encapsulation and cellular internalization of kombucha-derived bioactive compounds. This study provides a promising strategy for the development of value-added fermented beverages and functional food products. Full article

Beetroot pomace is a valuable food-processing by-product that is rich in betalains and phenolic compounds, but the instability of these bioactives limits their direct use in functional foods. This study aimed to develop a pumpkin seed protein-based encapsulated ingredient from beetroot pomace extract and evaluate its preliminary application in yogurt fortification. Beetroot pomace contained 193.75 ± 3.83 mg GAE/100 g DW of total phenolics and 95.78 ± 1.27 mg/100 g DW of total betalains. Encapsulation was optimized using the response surface methodology, with the wall-to-core ratio, extract dilution, and mixing time as independent variables. The optimal encapsulate showed experimentally confirmed encapsulation efficiencies of 75.37% for phenolics and 84.02% for betalains, containing 196.62 ± 4.37 mg GAE/100 g total phenolics and 53.19 ± 0.90 mg/100 g total betalains. After simulated gastrointestinal digestion, betalains remained detectable at 43.15 ± 1.46 mg/100 g, while total phenolics increased to 726.56 ± 30.59 mg GAE/100 g and DPPH antioxidant activity reached 1472.76 ± 7.58 mg TE/100 g, indicating the improved extractability of phenolics from the protein matrix. The encapsulate showed low water activity and moisture content but high hygroscopicity and very poor flowability, indicating the need for further powder-handling optimization. Yogurt fortification with 3% encapsulate, selected as a preliminary technologically feasible level, improved the bioactive profile during storage at 4 °C for 7 days and −18 °C for 21 days. These results support pumpkin seed protein-encapsulated beetroot pomace bioactives as sustainable multifunctional ingredients for yogurt fortification, while further sensory validation and comparison with free extracts are required. Full article

Climate change and population growth are intensifying global food security challenges by reducing agricultural productivity and increasing reliance on fertilizers. In this context, developing sustainable and economically efficient agricultural solutions becomes essential. The study presents the synthesis of an interpenetrating polymer network (IPN) of hydrogels by combining bacterial cellulose (BC) with poly(acrylic acid) crosslinked with N, N-methylene-bis-acrylamide (PAA–co–MBA) via free radical copolymerization. To explore their potential as bioactive compound carriers, an ethanolic hydroalcoholic phytoextract (EHP) obtained from Hypericum perforatum L. and Melissa officinalis L. was directly encapsulated within the IPN hydrogels. The EHP is valued for its rich bioactive profile and antifungal, antimycobacterial, and antioxidant properties. The results of rheology measurements and thermal gravimetric analysis (TGA) revealed that incorporating BC into the IPN hydrogels significantly enhanced the mechanical stiffness, thermal resistance, and overall stability of the resulting IPN structures. Fourier Transform Infrared (FTIR) spectroscopy and Scanning Electron Microscopy (SEM) confirmed the structural organization and the porosity of the developed composite, as well as the successful fabrication of IPN hydrogels in the EHP medium. Under optimal conditions, the IPN hydrogels exhibited a reduced swelling capacity, thereby slowing the diffusion of the bioactive agents, reducing the application frequency, and enhancing the utilization efficiency. Taken together with the controlled-release performance, these findings demonstrate the potential of BC (PAA-co-MBA) IPN hydrogels as biodegradable and sustainable carrier systems for controlled delivery applications and suggest that they may be promising candidates for hydrogel-based agricultural delivery systems. Full article

The increasing demand for clean-label meat products has accelerated interest in natural bioactive compounds, including essential oils, plant polyphenols, and bacteriocins, as alternatives to synthetic preservatives. These compounds have the potential to enhance product safety and shelf life while meeting consumer expectations. Many natural bioactives exhibit antioxidant and antimicrobial activities, enabling them to reduce lipid oxidation and inhibit the growth of spoilage and pathogenic microorganisms in meat systems. Despite these benefits, their practical application remains limited by instability, volatility, poor solubility, and undesirable sensory effects. Encapsulation technologies have emerged as effective approaches to overcome these limitations by enhancing stability, controlling release behavior, and improving compatibility with complex meat matrices. This review synthesizes evidence from 154 studies published between 2010 and 2026 on the application of encapsulation technologies, including microencapsulation, nanoemulsions, liposomes, and cyclodextrin-based systems, for natural bioactives in meat systems. Encapsulated bioactive delivery systems are evaluated by integrating spoilage mechanisms, delivery system design, and application strategies. Encapsulation approaches are discussed in terms of structure–function relationships, release behavior, and interactions with meat components. Application strategies, including direct incorporation, edible coatings, and active packaging, are comparatively analyzed based on their functional performance in meat systems. Overall, nanoscale delivery systems are particularly effective in improving the dispersion, stability, and functional performance of hydrophobic bioactives, while controlled-release systems offer prolonged protection but often exhibit reduced predictability when translated from model systems to real meat matrices. Current challenges related to scalability, cost, regulatory constraints, sensory impact, industrial implementation, and the safe design of sustained-release antimicrobial systems are also addressed, thereby providing a framework for the rational development and implementation of effective clean-label preservation strategies in meat systems. Full article

Background: Artemisia absinthium (A. absinthium) is a perennial plant valued for its antibacterial, antioxidant, and anti-inflammatory properties, exhibiting broader therapeutic potential. Given the need to deliver low doses of A. absinthium extract, mesoporous silica nanoparticles have attracted considerable attention as promising nanocarriers due to their distinctive physical and chemical properties. Methods: Physicochemical characterization of the materials was performed and biological assays were conducted to investigate the ROS, antibacterial and antioxidant activity of A. absinthium extract encapsulated within cerium- and calcium-doped mesoporous silica nanoparticles (MNSiCaCe) against both aerobic and anaerobic bacteria. Results: FTIR, SEM, and BET analysis confirmed successful synthesis of the MNSiCaCe. Phytochemical profiling of Artemisia absinthium extract using HPLC revealed the presence of artemisinin and a rich composition of phenolic and flavonoid constituents, with a total phenolic content of 182 ± 3.6 mg GAE/100 g dry plant material and a total flavonoid content of 42.5 ± 0.6 mg QE/100 g. Quantitative drug loading profiling demonstrated that while plain MNSi nanocarriers achieved a loading capacity of 16.96%, the MNSiCaCe enhanced this threshold to 43.11%. The in vitro controlled-release kinetics exhibited a highly prolonged and slow-release profile of the MNSiCaCe. The materials demonstrated excellent hemocompatibility and high mitochondrial activity with human periodontal ligament cells (hPDLCs). Elevated ROS generation was observed under conditions where antibacterial activity was most pronounced. While the artemisinin-doped nanoparticles showed notable antibacterial effects, the complete Artemisia absinthium-loaded nanoparticles achieved a significantly greater reduction in bacterial viability probably due to the synergistic interaction between artemisinin and the extract’s rich polyphenol profile. Conclusions: These findings highlight MNSiCaCe as a promising and safe nanocarrier system for drug delivery, with strong antibacterial potential, offering valuable applications in antibacterial therapies. Full article

Grape pomace (GP) is the main residue of the winemaking process; it is rich in phenolics with great bioactive potential. This study applied subcritical water extraction (SWE) to GP at 120, 140, and 160 °C to fractionate it into value-added products. The extraction performed at 120 °C and 1.3 bar (E-120) was the most efficient, resulting in the highest soluble fraction yield (61 g/100 g GP) with high phenolic content (4.1 g GAE (gallic acid equivalent)/100 g extract solids) and strong antioxidant capacity (EC₅₀: 0.79 mg extract/mg DPPH (2,2-Diphenyl-1-picrylhydrazyl)), while it inhibited the growth of Listeria innocua at 325 mg/mL. The insoluble extraction residue (38 g/100 g GP) also retained substantial phenolic content (10 g GAE/100 g), with high antioxidant activity (EC₅₀: 1.5 mg residue/mg DPPH) and a fibre-rich composition, supporting its value as a functional ingredient. To improve handling and stability, the E-120 extract was encapsulated in maltodextrin or pectin. For both polymers, a 40% polymer ratio with respect to the extract solids yielded a stable, single-phase, glassy powder (T_g ~50 °C), with thermal stability extending up to 200 °C. Therefore, SWE constitutes an efficient and sustainable technique for obtaining functional ingredients from GP, allowing for its valorisation. Full article

Diabetes mellitus (DM) is a highly prevalent metabolic disorder and a major public health concern. Pioglitazone, a widely used antidiabetic agent, exhibits limited therapeutic efficiency due to poor water solubility and suboptimal pharmacokinetic properties. Similarly, curcumin (Cur), a natural polyphenol with pleiotropic biological activities, is hindered by low oral bioavailability. In this study, chitosan-based nanocarriers were developed for the delivery of pioglitazone (CS-Pio NPs), curcumin (CS-Cur NPs), and their co-encapsulation (CS-Pio-Cur NPs), aiming to enhance their biological performance and therapeutic efficacy. The co-loaded nanosystem (CS-Pio-Cur NPs) demonstrated significantly enhanced antioxidant activity, as evidenced by DPPH (71.29 ± 0.09%), ABTS (86.08 ± 0.04%), and hydroxyl radical scavenging (87.08 ± 0.06%) assays, along with a strong reducing capacity (IC₅₀ = 25.39 ± 0.23 μg/mL). In a diabetic rat model, CS-Pio-Cur NPs significantly reduced blood glucose level and HbA1c (6.60 ± 0.83%), while also improving liver and kidney function parameters and lipid profile. These findings suggest that co-delivery of Pio and Cur via CS-based nanocarriers provides a combined therapeutic effect by simultaneously targeting hyperglycemia, oxidative stress, and associated metabolic dysfunctions. This nanosystem represents a promising approach for improving the management of DM and its complications. Full article

The development of functional yogurt products enriched with plant-derived ingredients and biologically active compounds represents a promising strategy to improve the nutritional, probiotic, and antioxidant properties of fermented dairy foods. The aim of this study was to evaluate how plant-derived ingredients (whole-meal flour from sprouted wheat grain and a protein-rich functional ingredient derived from hemp press cake), used individually or in combination with curcumin- or ergosterol-enriched Saccharomyces cerevisiae biomass, influence the physicochemical, structural-mechanical, probiotic, antioxidant, and sensory characteristics of yogurt products. Two forms of yeast biomass were used as enrichment agents: one containing encapsulated curcumin and the other with a high ergosterol content. Milk mixtures were supplemented with yeast biomass containing 34.0 mg/g encapsulated curcumin or 10.55 mg/g ergosterol. Additionally, whole-meal flour from sprouted wheat grain or the hemp-derived protein ingredient was incorporated into the yogurt products at concentrations of 2–3%. These ingredients were tested both individually and in combination to identify optimal formulations that would confer novel properties to the final products. Based on the conducted studies, it was found that the addition of enriched yeast biomass and the protein ingredient resulted in a denser and more uniform structure in the yogurt products compared to those of the control. The titratable acidity of the experimental formulations ranged from 80.2 to 91.8 °T, while pH values ranged from 3.79 to 4.04. Compared with the control sample, these changes indicate enhanced lactic acid fermentation activity. The number of probiotic microorganisms in the experimental samples reached 1.6 × 10⁷–6.4 × 10⁷ MPN/g, exceeding those of the control by an order of magnitude. The type of plant ingredient used significantly determined the technological properties of the finished product. Compared with the control sample, yogurt products supplemented with the hemp press cake-derived protein ingredient exhibited higher protein content (33–34% on a dry matter basis), increased viscosity (2.5–2.6 Pa·s), and reduced syneresis (values of 16.1 mL). The whole-meal flour from sprouted wheat grain exhibited a more pronounced stimulating effect on the growth of probiotic microflora. Enrichment of yogurt products with yeast biomass also increased antioxidant activity: the AOA (DPPH) value increased to 69–84% compared to ~62% in the control. Biotesting using Paramecium caudatum, a sensitive protozoan model widely used for rapid assessment of biological compatibility, toxicity, and the relative biological value of food systems, demonstrated a statistically significant increase (p < 0.05) in protozoan growth to 104–106% compared with the control sample, suggesting the absence of toxic effects and the potential bioavailability of yogurt matrix components. This data confirm the potential of using enriched yeast biomass in combination with plant ingredients for creating probiotic yogurt products with improved structural and functional properties. Full article

Iron deficiency and associated anemia remain major public health concerns, requiring innovative food fortification systems with adequate technological and sensory performance. This study aimed to develop a multicomponent fortified gummy candy using heme iron from Cavia porcellus erythrocytes, non-heme iron from Feranin^®, and elderberry juice, integrated through microencapsulation and stabilized by vacuum foam drying. Erythrocytes were isolated, dehydrated, and microencapsulated in a tara gum–maltodextrin matrix, yielding a powder with 1.49 mg Fe/g dry matter. The microencapsulates exhibited compact morphology, lower polydispersity, and negative ζ potential, indicating suitable surface stability. Three gummy formulations (F1–F3) were prepared with different proportions of encapsulated erythrocytes, non-heme iron, and elderberry juice. Iron content increased significantly from 0.21 to 0.89 mg Fe/g of gummy candy. The formulations showed variations in water activity (0.84–0.88), moisture (33.79–40.22%), pH (4.64–6.15), soluble solids (41.00–46.67 °Brix), phenolic compounds (0.80–1.14 mg GAE/g), flavonoids (0.13–0.27 mg QE/g), and antioxidant capacity (1.53–3.75 µmol TE/g). FTIR and SEM confirmed structural preservation and matrix integration. Sensory evaluation showed comparable overall acceptability among formulations, with F3 showing higher mean ranks for flavor and texture. Overall, vacuum foam drying was a feasible strategy for valorizing animal-derived by-products in fortified gelled confectionery. Full article

Developing biodegradable and functional polymeric materials for active food packaging is essential to mitigate the environmental burden of petroleum-based plastics. In this context, gelatin/chitosan (G–Ch) nanofibrous mats were fabricated via solution blow spinning (SBS) and functionalized with snail slime (SS) and Pinus sylvestris essential oil (PSEO) to enhance their bioactivity and barrier performance. SS is rich in glycoproteins and natural bioactive compounds, while PSEO is characterized by terpene-based antimicrobial and antioxidant activities. SS and PSEO were incorporated into the G–Ch polymeric matrix to enhance the bioactivity, structural functionality and preservation performance of the nanofibrous mats. Three formulations (G–Ch, G–Ch–SS, and G–Ch–SS–10PSEO) were designed to elucidate the influence of snail slime and essential oil incorporation on the structure–property–function relationships of the nanofibrous mats. Morphological analysis revealed a smooth and bead-free fibrous structure across all formulations. The average fiber diameter (AFD) increased from 191.83 nm for G–Ch to 263.88 nm for G–Ch–SS and 295.83 nm for G–Ch–SS–10PSEO. FTIR and XRD analyses showed the physical encapsulation of the active compounds without significant chemical interactions. Furthermore, the incorporation of PSEO increased surface hydrophobicity and reduced air permeability, indicating the formation of a more compact fibrous structure with enhanced barrier properties. The functional performance of the nanofibrous mats was significantly improved by the addition of snail slime and PSEO. The G–Ch–SS–10PSEO formulation exhibited the highest antioxidant activity, reaching 36.8% for DPPH and 42.7% for ABTS, along with enhanced antibacterial efficacy against both Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Application tests on chicken wings demonstrated that the bioactive nanofibers effectively suppressed microbial growth, limited pH increases, and reduced lipid oxidation during 14 days of refrigerated storage. Overall, the results demonstrate that the synergistic integration of snail slime and essential oil within a biodegradable polymer matrix provides a promising strategy for designing active nanofibrous materials with enhanced structural and bioactive properties for sustainable food-packaging applications. Full article