Search Results (757)

Background/Objectives: Facial and intraoral parameters are important guidelines in prosthetic planning and rehabilitation. This study aimed to analyze and determine the relationship between facial parameters and measurements on the upper anterior teeth using digital photography of the participants. Methods: This cross-sectional observational study included 82 student participants. Digital images (front facial and dental view) were taken of each participant, and then standardized images were used to measure facial and dental parameters. Results: The width of the maxillary anterior teeth and facial parameters were greater in males than in females, except for the medial canthus of the eye, which was slightly larger in females. A significant positive correlation was found between all facial parameters and the widths of the central and lateral incisors, as well as their combined sum. The strongest correlation was observed between the lateral canthus of the eye and the total width of the maxillary anterior teeth (r = 0.546; p < 0.001). In regression analysis, it was shown that the bizygomatic width had a statistically significant contribution to the prediction of the central incisor width (p = 0.045). It was also shown that the intraoral parameters, such as the height of the interdental papilla and interpapillary angle, are shape-dependent. Interincisal angles between the central incisors in all participants are significantly lower (p < 0.05) than the angles between incisal edges in other anterior teeth. Conclusions: Facial parameters cannot be used independently to predict dental parameters; nevertheless, when integrated with basic esthetic principles, they provide complementary information relevant to analytical procedures in restorative and prosthetic dentistry. Full article

Background/Objectives: To evaluate the incidence, characteristics, and clinical outcomes of intraocular inflammation (IOI) associated with intravitreal faricimab (IVF) in patients with neovascular age-related macular degeneration (nAMD) and diabetic macular edema (DME). Methods: Following PRISMA guidelines, a comprehensive search of PubMed, Web of Science, Scopus, Embase, and CENTRAL databases was performed from their inception to February 2025. Using the random-effects model, weighted proportions, standardized mean differences, and weighted log odds ratios (OR) were pooled and calculated. A two-tailed p-value of <0.05 was considered statistically significant. The χ² (z) test and the Higgins I² test were used to assess studies heterogeneity. Results: We conducted a systematic review and meta-analysis of 24 studies (4761 patients; 5652 eyes). The most common diagnoses were nAMD (n = 4782, 94.6%) and DME (n = 845, 37.1%). The pooled proportion for IOI incidence in eyes receiving IVF was 3.0% (95% CI: 1.0–6.0). The odds of developing IOI did not differ significantly between the DME and nAMD groups (OR: 1.13, p = 0.78). Unspecified IOI was the most common sign (n = 210, 2.9% [95% CI: 1.2–7.3]), followed by anterior uveitis (n = 80, 1.9% [95% CI: 0.1–34.8]), vitritis (n = 63, 2.9% [95% CI: 0.2–32.1]), retinal hemorrhage (n = 27, 0.7% [95% CI: 0.0–15.3]), and endophthalmitis (n = 8, 0.5% [95% CI: 0.3–1.1]). Conclusions: While IVF demonstrates therapeutic efficacy, our findings highlight a clinically relevant risk of IOI. We, therefore, recommend vigilant clinical monitoring in patients receiving this therapy. Full article

Objectives: To evaluate whether preoperative anterior segment optical coherence tomography (AS-OCT) parameters differ according to Big Bubble (BB) formation during deep anterior lamellar keratoplasty (DALK) in patients with TGFBI-related corneal stromal dystrophies (CSD). Methods: This retrospective cohort study included 17 eyes from 12 patients undergoing DALK with an attempted BB technique. Stromal deposit depth was assessed by AS-OCT using both a categorical depth-based classification (anterior, mid-, and posterior stroma) and continuous measurements of stromal involvement (µm). The ratio between stromal involvement and the thinnest corneal point was calculated. Intraoperative data included BB success, BB type, and complications. Inter-eye correlation was accounted for in comparisons of continuous variables using linear mixed-effects models. Results: BB formation was achieved in 11 of 17 eyes (64.7%), with type 1 BB observed in all successful cases. BB success was observed in all eyes with anterior or mid-stromal involvement and in 33.3% of eyes with posterior stromal involvement. Greater stromal deposit depth and a higher stromal-depth-to-thinnest-point ratio were observed in eyes in which BB formation failed (p < 0.01). No intraoperative perforations or conversions to penetrating keratoplasty occurred. Inter-observer agreement for AS-OCT measurements was high. Conclusions: BB failure was more frequent in eyes with greater absolute and relative stromal deposit depth, as assessed by preoperative AS-OCT during DALK in TGFBI-related CSD. These AS-OCT-derived parameters may support surgical planning and improve patient selection for BB DALK in this clinical setting. Full article

Background and Objectives: Keratoconus is a bilateral but often asymmetric ectatic corneal disease characterized by progressive thinning, increased curvature, and conical shape of the cornea. Previous studies have reported that the use of swimming goggles in patients with keratoconus can lead to increased intraocular pressure (IOP) and a transient reduction in anterior chamber volume (ACV), potentially affecting anterior segment morphology. This study aimed to evaluate the short-term effects of periorbital pressure induced by swimming goggles on corneal parameters in keratoconic eyes. Materials and Methods: A total of 44 eyes of 44 patients (mean age: 26.1 ± 5.1 years) diagnosed with keratoconus Stage 1–4 according to the Amsler–Krumeich classification were included. Measurements were taken using a Pentacam^® Scheimpflug camera before swimming goggle application and immediately after 20 min of wear. The parameters assessed included keratometry values (K1, K2, Km, Kmax), central and thinnest corneal thickness, corneal volume within the 10 mm zone (CV10), ACV, anterior chamber depth (ACD), iridocorneal angle (ICA), and pupil diameter (PD). Results: No statistically significant changes were observed in keratometric values, central and thinnest corneal thickness, ACV, ACD, ICA, PD, or IOP (all p > 0.05). CV10 showed a small reduction following goggle wear (Δ = −0.18 mm³, corresponding to a 0.3% decrease), which was statistically significant in the unadjusted analysis (p = 0.008) but did not remain significant after correction for multiple comparisons (p for false discovery rate [FDR] = 0.10). Conclusions: Short-term swimming goggle use may induce subtle reductions in CV10 in keratoconic eyes, suggesting a potential biomechanical sensitivity to transient periocular pressure. Although the observed change in CV10 did not retain statistical significance after multiple-comparison correction, it may reflect an early physiological response in structurally compromised corneas. CV measurements could serve as exploratory indicators of mechanical responsiveness in keratoconus, warranting further investigation in larger controlled studies. Full article

Background/Objectives: Infectious keratitis remains a major cause of blindness worldwide, and many cases progress to therapeutic keratoplasty despite advances in antimicrobial therapy. This systematic review aims to evaluate the outcomes of therapeutic keratoplasty in microbial keratitis and examine factors influencing anatomical success, graft survival, and visual rehabilitation. Methods: A systematic review was conducted following PRISMA guidelines, including English-language studies, published between 2000 and 2025. Studies with ≥10 eyes and ≥6 months follow-up were included. Data on infection control, graft clarity, anatomical success, visual acuity, and complications were extracted. Results: Fourteen studies encompassing 1527 eyes were analyzed. TPK accounted for 89% of procedures; DALK was used selectively for anterior or mid-stromal infections. Overall infection control ranged from 69 to 100%, with globe preservation in 85–100% of cases. Bacterial keratitis had higher cure rates and graft clarity than fungal or Acanthamoeba keratitis. Larger grafts (>8 mm) and deep stromal involvement were associated with increased graft rejection and postoperative complications. DALK offered higher graft survival and lower immunologic risk when the endothelium was spared. Visual outcomes were generally limited, reflecting preoperative disease severity, timing of surgery, and postoperative immunomodulation constraints. Early surgical intervention improved anatomical outcomes in severe fungal keratitis. Conclusions: Therapeutic keratoplasty is an effective globe-preserving intervention in advanced microbial keratitis, but with limited functional outcomes. Further prospective studies are needed to refine surgical indications, postoperative management, and long-term functional results. Full article

Background/Objectives: To evaluate the predictive accuracy of intraocular lens (IOL) power calculation by ray tracing in eyes with previous radial keratotomy (RK). Methods: A consecutive series of eyes with previous RK was retrospectively analyzed. Preoperatively, all eyes underwent optical biometry to measure the axial length (AL) and anterior segment imaging by the MS-39 (CSO), which combines Placido disk corneal topography and anterior segment optical coherence tomography. The built-in ray tracing software was used to calculate the IOL power. For comparative purposes, the results of the Barrett True-K, EVO, Haigis total keratometry, and PEARL-DGS formulas were also investigated. The refractive outcomes were evaluated with Eyetemis. Results: Twenty-four eyes (24 patients) were investigated. The mean AL and keratometry were, respectively, 27.34 ± 2.88 mm and 35.53 ± 3.66 diopters (D). The mean prediction error (PE) was −0.03 ± 0.65 D (range: from −1.30 to +1.64 D). The mean and median absolute errors were 0.52 and 0.48 D, respectively. The percentages of eyes with a PE within ±0.25 D, ±0.50 D, and ±1.00 D were 29.17%, 62.50%, and 87.50%, respectively. A comparison with the other formulas was possible in 20 eyes and did not reveal any statistically significant differences; the percentage of eyes with a PE within ±0.50 D ranged from 50 to 65%. Conclusions: Ray tracing is a relatively accurate solution for calculating the IOL power in eyes with previous RK. Paraxial formulas provide similar outcomes and should be considered in these patients. The refractive outcomes of IOL power calculation in post-RK eyes are still below modern benchmarks for virgin eyes. Full article

Background: Juvenile idiopathic arthritis-associated uveitis (JIA-U) is a rare condition, and assessment of the efficacy of disease-modifying antirheumatic drugs, synthetic (sDMARD) or biological (bDMARD), in randomized trials is hindered by this fact. Methods: In this prospective longitudinal study, we observed 38 children aged 1.3 to 15.2 years, with 69 eyes affected with JIA-U for 1970 overall eye examinations (6–59, median 16) irregularly scattered across 4.4–87.6 months (median 21.6) of follow-up, with on- and off-periods of DMARD use and use of topical treatments. Results: With adjustment for several time-invariant and time-varying covariates, periods of exposure to sDMARD vs. no DMARD exposure were associated with peak benefits of 15–20% lower probability of having more severe anterior chamber (AC) inflammation and a similar relative reduction in the daily use of topical corticosteroids (TCS). Periods of bDMARD exposure or of bDMARD + sDMARD exposure vs. no DMARD use were associated with peak benefits of an around 50% reduction in the probability of having more severe AC inflammation, and peak benefits of an around 60–65% reduction in TCS use. Conclusions: The observations regarding bDMARD (only) or bDMARD + sDMARD exposure are in agreement with the extent of benefits suggested for adalimumab vs. placebo (+background sDMARD) in the only existing randomized trial in this setting evaluating AC inflammation and TCS use. Full article

Background/Objectives: Keratoconus, a condition in which the cornea becomes thinner and steeper, can cause visual problems, particularly when it is progressive. Early diagnosis is important for preserving visual acuity. Raw data, unlike preprocessed data, are unaffected by software modifications. They retain their native structure across versions, providing consistency for analytical purposes. The objective of this study was to design a deep learning-based graphical user interface for predicting the corneal ectasia score using raw optical coherence tomography data. Methods: The graphical user interface was developed using Tkinter, a Python library for building graphical user interfaces. The user is allowed to select raw data from the cornea/anterior segment optical coherence tomography Casia2, which is generated in the 3dv format, from the local system. To view the predicted corneal ectasia score, the user must determine whether the selected 3dv file corresponds to the left or right eye. Extracted optical coherence tomography images are cropped, resized to 224 × 224 pixels and processed by the modified EfficientNet-B0 convolutional neural network to predict the corneal ectasia score. The predicted corneal ectasia score value is displayed along with a diagnosis: ‘No detectable ectasia pattern’ or ‘Suspected ectasia’ or ‘Clinical ectasia’. Performance metric values were rounded to four decimal places, and the mean absolute error value was rounded to two decimal places. Results: The modified EfficientNet-B0 obtained a mean absolute error of 6.65 when evaluated on the test dataset. For the two-class classification, it achieved an accuracy of 87.96%, a sensitivity of 82.41%, a specificity of 96.69%, a positive predictive value of 97.52% and an F1 score of 89.33%. For the three-class classification, it attained a weighted-average F1 score of 84.95% and an overall accuracy of 84.75%. Conclusions: The graphical user interface outputs numerical ectasia scores, which improves other categorical labels. The graphical user interface enables consistent diagnostics, regardless of software updates, by using raw data from the Casia2. The successful use of raw optical coherence tomography data indicates the potential for raw optical coherence tomography data to be used, rather than preprocessed optical coherence tomography data, for diagnosing keratoconus. Full article

Background/Objectives: Surgery performed in the steep Trendelenburg position is considered potentially detrimental to ocular structures. This study aimed to evaluate morphological changes in the anterior segment during robot-assisted laparoscopic prostatectomy (RALP). Methods: This was a single-center, prospective observational study involving 60 eyes of 30 consecutive patients undergoing RALP between May and November 2021. Anterior segment images were obtained using a Smart Eye Camera before surgery (supine and awake), during surgery (supine and steep Trendelenburg under anesthesia), and after surgery (supine). Assessed parameters included the iridocorneal angle, the ratio of peripheral anterior chamber depth to peripheral corneal thickness based on Van Herick Plus grading, and pupil diameter. Each parameter in the steep Trendelenburg position under anesthesia was compared with measurements obtained in the supine position under anesthesia and in the awake condition. The primary outcome was the comparison of anterior segment morphological changes between the supine and steep Trendelenburg positions during RALP. Results: Upon transition to the steep Trendelenburg position, anterior chamber depth significantly decreased (p < 0.001), recovering after returning to the supine position. The proportion of eyes classified as having narrowed anterior chambers increased significantly (p < 0.001), with more than 60% showing shallower chambers relative to preoperative measurements. Extreme anterior chamber narrowing occurred in 3 out of 410 intraoperative assessments (1%). Pupils were uniformly constricted under anesthesia. Conclusions: Steep Trendelenburg positioning significantly reduces anterior chamber depth. This morphological alteration may contribute to the marked increase in intraocular pressure observed during RALP. Full article

Background/Objectives: Hemodialysis (HD) is the commonest life sustaining form of kidney replacement therapy in the world; however, this method of treatment have many adverse effects, and even a single HD session affects many organs, including the eyes. The aim of this study was to assess the effect of a single HD session on the ophthalmologic findings in patients with End-stage Renal Disease (ESRD). The second aim of the study was to examine the correlation of these changes with each other and between changes in systemic stressors related to the HD session. Methods: This was a single-center cross-sectional observational study conducted on 32 patients undergoing HD. Selected parameters of the anterior and posterior segment of the eye as well as systemic parameters were assessed before and after a single HD session. Results: Best corrected visual acuity (BCVA) improved, and lens thickness (LT), axial length (AXL), average macular thickness (MT), central MT and total vessel density (VD) of the deep capillary plexus DCP increased significantly after a single HD session. The Schirmer test results, tear break up time (TBUT), anterior chamber depth (ACD), central and average choroidal thickness (CT) decreased significantly after HD. Body weight loss was the only significant systemic change. Decrease in TBUT correlated positively with Schirmer’s test results decrease. Increase in CCT correlated positively with AXL increase. Decrease in central and average CT correlated positively with IOP decrease. Increase in central MT correlated positively with increase in average MT. Decrease in central CT correlated positively with average CT decrease. Change in VD of the SCP correlated positively with change in VD of DCP. Apart from the positive correlation between SBP change and Schirmer’s test results change, there were no correlations between systemic and ophthalmic parameters changes. Conclusions: Our study showed that HD affected the parameters of the anterior and posterior segments of the eye. Numerous correlations between these changes suggest that they are interrelated and represent the complex response of the eye to the HD process. Full article

Background: Descemet membrane endothelial keratoplasty (DMEK) is the most frequently performed keratoplasty procedure in many countries. One of the most common early complications is an elevation of intraocular pressure (IOP). The aim of this study was to characterize early postoperative IOP behavior following DMEK performed with 10% sulfur hexafluoride (SF₆) tamponade and to determine the frequency and timing of required IOP-lowering interventions within the first 48 h. Methods: We retrospectively reviewed postoperative outcomes of 116 consecutive DMEK procedures between May and December 2024 at the University Medical Center in Mainz, Germany. No specific exclusion criteria were applied. All surgeries included a surgical iridectomy at the 6 o’clock position, 10% (SF₆) tamponade, and maintaining a mid-normal IOP at the end of surgery. Postoperative assessments included IOP measured using Goldmann applanation tonometry, the percentage of gas fill in the anterior chamber evaluated at the slit lamp, and the need for IOP-lowering interventions as determined by the on-call resident at 3, 24, and 48 h after surgery. IOP-lowering interventions consisted of venting in cases of elevated IOP, gas fill > 90%, and/or suspected angle closure or pupillary block, as well as intravenous or oral acetazolamide in cases of moderate IOP elevation with a lower gas fill and a patent iridectomy. If a single intervention was insufficient, a combined approach was used. Results: A total of 116 eyes from 98 patients (62 female, mean age 73.0 ± 9.8 years) were analyzed. DMEK was combined with cataract surgery in 41 eyes, and 4 eyes underwent phakic DMEK. Postoperatively, all iridectomies remained patent, and no cases of pupillary block occurred. Mean IOP and gas fill were within normal limits and declined steadily during the first 48 h. IOP-lowering procedures were performed in 11 eyes (9.5%), including venting (n = 3), acetazolamide administration (n = 7), and a combination of both (n = 1). There was no difference between DMEK and triple-DMEK regarding postoperative gas fill, IOP, or the need for IOP-lowering interventions. Mean postoperative IOP was significantly higher, and IOP-lowering interventions were more frequent in glaucoma vs. non-glaucoma patients. Re-bubbling was performed in 12 eyes (10.3%). Two cases of primary graft failure (1.7%) were recorded. Conclusions: In our patient cohort, a standardized surgical approach incorporating a surgical iridectomy at the 6 o’clock position, 10% SF₆ tamponade, and maintaining a mid-normal IOP at the end of surgery effectively prevented pupillary block. We recommend early postoperative assessment of IOP and percent gas fill to promptly identify and manage impending IOP elevation, which is particularly important in patients with glaucoma. Full article

Establishing baseline ultrasound biomicroscopy (UBM) measurements of the feline anterior segment is essential for accurate evaluation of ocular diseases. This study aimed to establish normative UBM parameters in clinically normal cats and to assess the influence of sex, body weight, and age on these measurements. Twenty eyes from twenty healthy adult cats were examined using a standardized UBM protocol under general anesthesia to ensure consistent positioning and optimal visualization. Quantitative measurements were obtained for corneal, anterior chamber, iris, ciliary cleft, and ciliary body parameters. The mean perilimbal corneal thickness was 0.59 mm, anterior chamber depth was 4.11 mm, and peripheral anterior chamber depth was 3.11 mm. The angle-opening distance and iridocorneal angle measured 1.73 mm and 28.89°, respectively. Ciliary cleft parameters included a width of 1.22 mm, length of 2.07 mm, and area of 1.10 mm². Longitudinal and combined longitudinal–radial ciliary body thicknesses were 0.52 mm and 0.78 mm, respectively, while ciliary body axial length and ciliary process scleral angle averaged 2.01 mm and 58.98°. Iris base width and iris middle width measured 0.35 mm and 0.54 mm, respectively. Sex-based analysis revealed significant differences in peripheral anterior chamber depth and ciliary cleft width, whereas body weight showed positive correlations with iris thickness parameters. No significant associations were identified between age and any anterior segment measurements. These findings establish comprehensive normative UBM reference values for the feline anterior segment and provide a quantitative anatomical framework for future clinical and disease-oriented studies in feline ophthalmology. Full article

Purpose: The purpose of this study is to evaluate changes in corneal thickness and anterior and posterior corneal curvature after one year of scleral lens wear in keratoconus eyes and to determine their impact on visual performance. Methods: Sixty-five keratoconus subjects were divided into two groups: with intrastromal corneal ring segments (KC-ICRS) and without ICRS (KC). All participants wore 16.5 mm scleral lenses for 8 h daily over 1 year. Measurements included corneal thickness, anterior and posterior curvature, and high-contrast visual acuity assessed before and after lens wear. Results: Corneal thicknesses increased significantly in the superior region of the KC-ICRS group. In curvature analysis, the KC group showed inferior steepening and superior flattening, while the KC-ICRS group exhibited central and superior-nasal anterior flattening. Posterior curvature changes included central flattening and peripheral steepening. Visual acuity remained stable across all visits and groups. Conclusions: Long-term scleral lens wear induced measurable morphological changes, including increased superior corneal thickness and region-specific curvature alterations, which varied by ICRS presence. These changes did not compromise visual acuity, supporting scleral lenses as a safe and effective option for sustained vision correction in keratoconus. The findings highlight the importance of personalized fitting and monitoring strategies in clinical practice. Full article

Background: Active-fluidics phacoemulsification can maintain anterior chamber stability at lower intraoperative intraocular pressure (IOP) levels. However, whether reducing IOP alone—without additional stabilizing technologies such as the Active Sentry handpiece—can decrease surgical invasiveness during Eight-Chop phacoemulsification remains unclear. Methods: In this prospective fellow-eye comparative study, 56 non-diabetic patients (112 eyes) underwent Eight-Chop technique phacoemulsification using the Centurion Vision System with active fluidics. One eye was randomly assigned to a standard-IOP setting (55 mmHg; high-IOP group) and the fellow eye to a reduced-IOP setting (28 mmHg; low-IOP group). Intraoperative parameters—including operative time, phaco time, aspiration time, cumulative dissipated energy (CDE), and irrigation volume—were recorded. Postoperative outcomes included aqueous flare (laser flare photometry), corneal endothelial cell density (CECD) and CECD loss, corneal morphology (central corneal thickness [CCT], coefficient of variation [CV], percentage of hexagonal cells [PHC]), and IOP. Linear mixed-effects models with patient ID as a random effect were used for all paired-eye comparisons. Results: Lowering the intraoperative IOP did not reduce surgical invasiveness. Phaco time was significantly longer in the low-IOP group (16.2 ± 5.22 s vs. 13.9 ± 4.40 s; p = 0.001), and aspiration time was also longer (75.0 ± 18.3 s vs. 69.0 ± 17.9 s; p = 0.033). No significant differences were found in operative time (5.08 ± 1.10 min vs. 4.82 ± 1.13 min; p = 0.082), CDE (5.93 ± 1.87 vs. 5.56 ± 1.90; p = 0.099), or irrigation volume (26.6 ± 7.71 mL vs. 25.2 ± 7.35 mL; p = 0.214). Postoperative outcomes were similarly comparable. Aqueous flare showed no significant differences at any time point (e.g., day 1: 14.8 ± 5.10 vs. 14.5 ± 4.76 ph/ms; p = 0.655). Mean CECD loss remained small in both groups and did not differ significantly (7 weeks: −0.82 ± 1.05% vs. −0.98 ± 1.16%, p = 0.460; 19 weeks: −0.93 ± 1.38% vs. −1.28 ± 1.69%, p = 0.239). Corneal morphological parameters (CCT, CV, PHC) and postoperative IOP also showed no significant differences between settings. Conclusions: In this fellow-eye comparative study, lowering intraoperative intraocular pressure from conventional to near-physiologic levels under active-fluidics control did not reduce surgical invasiveness during Eight-chop phacoemulsification. No additional benefits were observed in terms of endothelial cell preservation, postoperative inflammation, or overall surgical performance. These findings indicate that, when chamber stability is already ensured by a low-invasive fragmentation strategy, further reduction in intraoperative IOP alone does not confer measurable short-term clinical advantages. Full article

Background/Objectives: Polymerase chain reaction (PCR) testing of ocular fluids is an essential diagnostic method for identifying infectious causes of uveitis. However, multiplex PCR kits specifically developed for ophthalmic use are not commercially available in many regions, including Korea. Given the biochemical similarity between cerebrospinal fluid (CSF) and aqueous humor, this study evaluated the diagnostic utility of a commercially available CSF multiplex PCR panel for detecting herpesviruses in patients with suspected viral uveitis. Methods: We retrospectively reviewed the medical records of patients whose aqueous humor samples were analyzed using a multiplex PCR assay originally designed for CSF testing (Seeplex Meningitis-V1 ACE Detection kit, Seegene, Seoul, Republic of Korea). The samples were obtained between May 2019 and June 2023 at two tertiary referral hospitals. The assay targeted herpes simplex virus types 1 and 2 (HSV-1, HSV-2), varicella-zoster virus (VZV), cytomegalovirus (CMV), Epstein–Barr virus (EBV), and human herpesvirus 6 (HHV-6). Patients were classified into three groups: (I) anterior uveitis with suspected herpesviral infection, (II) acute retinal necrosis (ARN), and (III) CMV retinitis. Baseline characteristics, PCR positivity rates, and virus prevalence were compared among the groups. Results: Among 149 eyes tested, 86 were included in the final analysis. The overall positivity rate was 38.4%. PCR positivity was 19.7% (12/61) in Group I, 93.8% (15/16) in Group II, and 66.7% (6/9) in Group III. CMV was the most common pathogen in Groups I (66.7%) and III (100%), while VZV was predominant in Group II (80%). No HHV-6 infection was detected. Conclusions: The positivity rate in anterior uveitis (Group I) was lower than previously reported, likely due to the limited sample volume relative to the assay’s requirement. Nevertheless, the assay demonstrated diagnostic reliability comparable to previous reports for ARN and CMV retinitis. Therefore, the CSF-based multiplex PCR panel serves as a feasible and cost-effective diagnostic option for sight-threatening posterior segment infections, facilitating prompt diagnosis and treatment, although further optimization is warranted for anterior uveitis. Full article