Search Results (277)

This study presents the scientific and methodological foundation of Spain’s first national framework for the ethical management of community cat populations: the Action Plan for the Management of Community Cat Colonies (PACF), launched in 2025 under the mandate of Law 7/2023. This pioneering legislation introduces a standardized, nationwide obligation for trap–neuter–return (TNR)-based management of free-roaming cats, defined as animals living freely, territorially attached, and with limited socialization toward humans. The PACF aims to support municipalities in implementing this mandate through evidence-based strategies that integrate animal welfare, biodiversity protection, and public health objectives. Using standardized data submitted by 1128 municipalities (13.9% of Spain’s total), we estimated a baseline population of 1.81 million community cats distributed across 125,000 colonies. These data were stratified by municipal population size and applied to national census figures to generate a model-ready demographic structure. We then implemented a stochastic simulation using Vortex software to project long-term population dynamics over a 25-year horizon. The model integrated eight demographic–environmental scenarios defined by a combination of urban–rural classification and ecological reproductive potential based on photoperiod and winter temperature. Parameters included reproductive output, mortality, sterilization coverage, abandonment and adoption rates, stochastic catastrophic events, and territorial carrying capacity. Under current sterilization rates (~20%), our projections indicate that Spain’s community cat population could surpass 5 million individuals by 2050, saturating ecological and social thresholds within a decade. In contrast, a differentiated sterilization strategy aligned with territorial reproductive intensity (50% in most areas, 60–70% in high-pressure zones) achieves population stabilization by 2030 at approximately 1.5 million cats, followed by a gradual long-term decline. This scenario prioritizes feasibility while substantially reducing reproductive output, particularly in rural and high-intensity contexts. The PACF combines stratified demographic modeling with spatial sensitivity, offering a flexible framework adaptable to local conditions. It incorporates One Health principles and introduces tools for adaptive management, including digital monitoring platforms and standardized welfare protocols. While ecological impacts were not directly assessed, the proposed demographic stabilization is designed to mitigate population-driven risks to biodiversity and public health without relying on lethal control. By integrating legal mandates, stratified modeling, and realistic intervention goals, this study outlines a replicable and scalable framework for coordinated action across administrative levels. It exemplifies how national policy can be operationalized through data-driven, territorially sensitive planning tools. The findings support the strategic deployment of TNR-based programs across diverse municipal contexts, providing a model for other countries seeking to align animal welfare policy with ecological planning under a multi-level governance perspective. Full article

Animal protein production represents a complex system of lives transformed into nutrition, with profound ethical and environmental implications. This study provides a quantitative analysis of animal lives required to produce human-consumable protein across major food production systems. Categorizing animal lives based on cognitive complexity and accounting for all lives involved in production, including direct harvests, reproductive animals, and feed species, reveals dramatic variations in protein efficiency. The analysis considers two categories of animal life: complex-cognitive lives (e.g., mammals, birds, cephalopods) and pain-capable lives (e.g., fish, crustaceans). Calculating protein yield per life demonstrates efficiency differences spanning more than five orders of magnitude, from 2 g per complex-cognitive life for baby octopus to 390,000 g per life for bovine dairy systems. Key findings expose disparities between terrestrial and marine protein production. Terrestrial systems involving mammals and birds show higher protein yields and exclusively involve complex-cognitive lives, while marine systems rely predominantly on pain-capable lives across complex food chains. Dairy production emerges as the most efficient system. Aquaculture systems reveal complex dynamics, with farmed carnivorous fish requiring hundreds of feed fish lives to produce protein, compared to omnivorous species that demonstrate improved efficiency. Beyond quantitative analysis, this research provides a framework for understanding the ethical and ecological dimensions of protein production, offering insights for potential systemic innovations. Full article

Introduction: Given the impact of aneurysmal subarachnoid hemorrhage (aSAH) on patients’ health, preclinical research is substantial to understand its pathophysiology and improve treatment strategies, which necessitates reliable and comprehensive animal models. Traditionally, aSAH models utilize iliac or subclavian access for angiography, requiring invasive procedures that are associated with significant risks and animal burden. This pilot study explores a less invasive method of digital subtraction angiography (DSA) by using the auricular artery (AA) as an alternative access point. Our aim was to demonstrate the feasibility of this refined technique, with the intention of reducing procedural risks, providing shorter operation times with enhanced neurological recovery, and simplifying the process for both researchers and animals. Materials and Methods: In this study, six female New Zealand white rabbits (3.2–4.1 kg body weight) underwent experimental induction of aSAH via a subclavian-cisternal shunt. The initial steps of this procedure followed traditional techniques, consisting of subclavian access through microsurgical preparation, followed by DSA to analyze retrograde filling of the basilar artery (BA). To evaluate the alternative method, on day 3 after induction of aSAH, DSA was performed via the AA instead of the traditional subclavian or femoral access. A catheter was placed in the AA to allow retrograde filling of the BA. This approach aimed to simplify the procedure while maintaining comparable imaging quality. Results: All rabbits survived until the study endpoint. Postoperatively, two rabbits showed signs of hemisyndrome, which significantly improved by the time of follow-up. No additional morbidities were observed. Upon euthanasia and necropsy, all animals showed clear subarachnoid bleeding patterns. DSA via the AA produced strong contrasting of the BA comparable to the traditional method. Conclusions: This technical note presents an initial evaluation of AA access as a feasible and potentially advantageous method for DSA in a rabbit model of blood shunt subarachnoid hemorrhage. The method shows promise in reducing invasiveness and procedural complexity, but further studies are required to fully establish its efficacy and safety. Future research should focus on expanding the sample size, refining the anatomical understanding of the AA, and continuing to align with ethical considerations regarding animal welfare. Full article

Ensuring sustainable food systems is an urgent global priority as populations grow and environmental pressures mount. Technological innovations such as genetic engineering (GE) and nanotechnology (nano) have been promoted as promising pathways for achieving greater sustainability in agriculture and food production. Yet, the sustainability of these technologies is not defined by technical performance alone; it hinges on how they are perceived by key stakeholders and how well they align with broader societal values. This study addresses the critical question of how expert stakeholders evaluate the sustainability of GE and nano-based food and agriculture (agrifood) products. Using a multi-method online platform, we engaged 42 experts across academia, government, industry, and NGOs in the United States to assess six real-world case studies—three using GE and three using nano—across ten different dimensions of sustainability. We show that nano-based products were consistently rated more favorably than their GE counterparts in terms of environmental, economic, and social sustainability, as well as across ethical and societal dimensions. Like prior studies, our results reveal that stakeholders see meaningful distinctions between nanotechnology and biotechnology, likely due to underlying value-based concerns about animal welfare, perceived naturalness, or corporate control of agrifood systems. The fruit coating and flu vaccine—both nano-enabled—received the most positive ratings, while GE mustard greens and salmon were the most polarizing. These results underscore the importance of incorporating stakeholder perspectives in technology assessment and innovation governance. These results also suggest that responsible innovation efforts in agrifood systems should prioritize communication, addressing meaningful societal needs, and the contextual understanding of societal values to build trust and legitimacy. Full article

Caring for the lives and welfare of animals is central to veterinary and animal sanctuary work, yet the meaning remains a subject of complex debates. Different stakeholders negotiate what constitutes appropriate care, leading to conflicting demands and expectations from internal and external sources. This article is based on two qualitative studies: Study I explores the multifaceted aspects of death work in farm animal medicine, emphasising the practical, emotional and ethical challenges involved. Study II examines human–animal interaction in sanctuaries, which reveal tensions between instrumental and relational care in animal-centred work. Relational care represents a subjectifying approach with individual attention to animals, while instrumental care is a more objectifying perspective based on species representation. These demands can often be contradictory, complicating day-to-day decision making under pressure. To analyse these complexities, this study employs Clarke’s situational analysis (social worlds/arenas mapping), providing a means of comparing care work across different fields. This approach highlights how actor constellations, institutional settings, and structural constraints influence the negotiation of care. Addressing these issues provides a more nuanced understanding of the professional challenges of animal-centred care and the necessary skills to navigate its inherent contradictions. Full article

The COVID-19 pandemic has significantly altered consumer habits, particularly in relation to food choices. In this context, plant-based diets have gained prominence, driven by health, environmental, and ethical considerations. This study investigates the primary motivational and inhibitory factors influencing the consumption of plant-based foods among residents of the Galicia–Northern Portugal Euroregion. Utilizing the Theory of Reasoned Action, an extended model was proposed and tested through a quantitative survey. A total of 214 valid responses were collected via an online questionnaire distributed in Portuguese and Spanish. Linear regression analysis revealed that health awareness, animal welfare, and environmental concern significantly shape positive attitudes, which subsequently affect the intention to consume plant-based foods. Additionally, perceived barriers—such as lack of taste and insufficient information—were found to negatively influence intention. These findings contribute to the consumer behavior literature and provide strategic insights for stakeholders aiming to promote more sustainable dietary patterns in culturally connected cross-border regions. Full article

The growing recognition of animal sentience has led to notable progress in European Union animal welfare legislation. However, a significant inconsistency remains: while mammals, birds, and cephalopods are legally protected as sentient beings, honeybees (Apis mellifera)—despite robust scientific evidence of their cognitive, emotional, and sensory complexity—are excluded from such protections. This manuscript examines, from an interdisciplinary perspective, the divergence between emerging evidence on invertebrate sentience and current EU legal frameworks. Honeybees and cephalopods serve as comparative case studies to assess inconsistencies in the criteria for legal recognition of sentience. Findings increasingly confirm that honeybees exhibit advanced cognitive functions, emotional states, and behavioral flexibility comparable to those of legally protected vertebrates. Their omission from welfare legislation lacks scientific justification and raises ethical and ecological concerns, especially given their central role in pollination and ecosystem stability. In general, we advocate for the inclusion of Apis mellifera in EU animal welfare policy. However, we are aware that there are also critical views on their introduction, which we address in a dedicated paragraph of the manuscript. For this reason, we advocate a gradual and evidence-based approach, guided by a permanent observatory, which could ensure that legislation evolves in parallel with scientific understanding, promoting ethical consistency, sustainable agriculture, and integrated health under the One Health framework. This approach would meet the concerns of consumers who consider well-being and respect for the environment as essential principles of breeding, and who carefully choose products from animals raised with systems that respect welfare, with indisputable economic advantages for the beekeeper. Full article

This paper explores responses to online coverage of an avian tracking project. Researchers attached novel trackers to a small group of wild magpies (Gymnorhina tibicen). These were subsequently removed by conspecifics, an example of ‘rescue behavior’ that was recounted in several media outlets. Online comments on three articles, from across the political spectrum (the Conversation, UK Guardian, and UK Daily Mail), were selected for thematic analysis. The resulting 680 comments were analyzed qualitatively and quantitatively to uncover predominant themes and the overall balance of positive and negative sentiments expressed about this tagging project or wildlife tagging generally. Topics occurring most frequently were themed into three interrelated areas: (1) sharing personal feelings and experiences, (2) comparing the merits of different species, and (3) sharing knowledge and opinion. Twenty-one percent (21%) of respondents expressed an opinion on the ethics of wildlife tagging. In the Daily Mail and Guardian, this opinion was more likely to be negative towards the use of tags. Opinion was more balanced for readers of the Conversation’s article. Willingness to comment on online news is low, and readers of this story were not asked directly for their opinion. Nevertheless, the data here illustrate some public perceptions of wildlife tagging, and there was a clear negative reaction from many responders. Widening the means through which people can engage with animal science has the potential to advance discussions around research ethics and animal welfare. Reactions to this story expose important questions for scientists seeking to engage with, and convince, the public of the merits of their work. Full article

Although consumption is a key engine of economic growth, it is also one of the main drivers of environmental degradation. In this context, a deeper understanding of consumers’ perceptions and behavior regarding sustainable products is essential for promoting a more sustainable future, with behavioral change being crucial to achieving it. This research study explores consumer behavior regarding sustainable egg consumption, a sector where production is frequently associated with environmental and ethical concerns such as animal welfare and intensive resource use. Based on a sample of 197 valid responses from Portuguese egg consumers collected through an online survey distributed between 27 February and 17 March, of the year 2025, and applying the Theory of Planned Behavior (TPB) framework through a structural equation modeling (SEM) approach, this study finds that consumers’ intention to consume sustainable eggs is influenced by their attitudes and subjective norms. Furthermore, actual consumption behavior is driven by both the intention to consume sustainable eggs and perceived behavioral control. By providing an in-depth analysis of consumer behavior related to sustainable egg consumption, this study contributes to the advancement of the scientific literature on the subject. It also aims to support policymakers and other stakeholders in designing and implementing more effective strategies to promote environmentally responsible consumption practices. Full article

The contemporary food environment is experiencing substantial transformations, and sustainability and the pursuit of novel ingredients have become pivotal factors in consumer decision-making and business strategy formulation. The emergence of alternative proteins has significant implications for sustainability, climate change mitigation and animal welfare, and can potentially promote the achievement of different United Nations Sustainable Development Goals. The spread of these novel foods requires significant attention, particularly concerning younger demographics, which are often considered to be more environmentally and ethically aware when making dietary choices. This study investigates the psychological and attitudinal factors that shape young consumers’ intention to consume plant-based, insect-based, and cultured meat alternative proteins, and verifies the existence of heterogeneous segments of consumers. To this purpose principal component analysis and k-means cluster analysis are employed using data from an online survey conducted in Italy, with a sample of 350 young consumers. The findings reveal that the intention to consume alternative protein sources is not particularly pronounced among the young consumers and differs according to the type of alternative protein. Three different profiles of young consumers were found: traditionalist (39% of the sample); enthusiastic (17%) and open-minded (44%). These segments differ significantly with respect to consumption intention, socio-demographic, lifestyle and psychological variables. The results provide useful insights for policymakers to design targeted interventions to encourage young consumers’ sustainable food choices. The findings could also be useful for marketers interested in investing in such innovations as they can benefit from an in-depth knowledge of this market segment. Full article

This study presents a bibliometric review aimed at mapping and analyzing the scientific literature related to the ethical implications of artificial intelligence (AI) in livestock farming, which is a rapidly emerging yet still underexplored field in international research. Based on the Scopus database, 151 documents published between 2015 and 2025 were identified and analyzed using the VOSviewer version 1.6.20 and Biblioshiny for Bibliometrix (RStudio version 2023.12.1) tools. The results show a significant increase in publications from 2021 onwards, reflecting the growing maturity of discussions around the integration of digital technologies in the agricultural sector. Keyword co-occurrence and bibliographic coupling analyses revealed the formation of four main thematic clusters, covering technical applications in precision livestock farming as well as reflections on governance, animal welfare, and algorithmic justice. The most influential authors, high-impact journals, and leading countries in the field were also identified. As a key contribution, this study highlights the lack of robust ethical guidelines and proposes future research directions for the development of regulatory frameworks, codes of conduct, and interdisciplinary approaches. The findings underscore the importance of aligning technological innovation with ethical responsibility and social inclusion in the transition to digital livestock farming. Full article

The recent birth of genetically modified canids phenotypically resembling the extinct dire wolf (Aenocyon dirus) was hailed as a landmark in synthetic biology. Using genome editing and cloning, the biotech company Colossal Biosciences created three such animals from gray wolf cells, describing the project as an effort in “functional de-extinction”. This case raises significant questions regarding animal welfare, moral justification, and regulatory governance. We used the five domains model framework to assess the welfare risks for the engineered animals, the surrogate mothers used in reproduction, and other animals potentially affected by future reintroduction or escape scenarios. Ethical implications are examined through utilitarian, deontological, virtue, relational, and environmental ethics. Our analysis suggests that the project suffers from ontological ambiguity: it is unclear whether the animals created are resurrected species, hybrids, or novel organisms. While the current welfare of the engineered animals may be manageable, their long-term well-being, particularly under rewilding scenarios, is likely to be compromised. The moral arguments for reviving long-extinct species are weak, particularly in cases where extinction was not anthropogenic. Legally, the current EU frameworks lack the clarity and scope to classify, regulate, or protect genetically engineered extinct animals. We recommend that functional de-extinction involving sentient beings be approached with caution, supported by revised welfare tools and regulatory mechanisms. Full article

This review critically analyzes recent advancements in dairy cow behavior recognition, highlighting novel methodological contributions through the integration of advanced artificial intelligence (AI) techniques such as transformer models and multi-view tracking with social network analysis (SNA). Such integration offers transformative opportunities for improving dairy cattle welfare, but current applications remain limited. We describe the transition from manual, observer-based assessments to automated, scalable methods using convolutional neural networks (CNNs), spatio-temporal models, and attention mechanisms. Although object detection models, including You Only Look Once (YOLO), EfficientDet, and sequence models, such as Bidirectional Long Short-Term Memory (BiLSTM) and Convolutional Long Short-Term Memory (convLSTM), have improved detection and classification, significant challenges remain, including occlusions, annotation bottlenecks, dataset diversity, and limited generalizability. Existing interaction inference methods rely heavily on distance-based approximations (i.e., assuming that proximity implies social interaction), lacking the semantic depth essential for comprehensive SNA. To address this, we propose innovative methodological intersections such as pose-aware SNA frameworks and multi-camera fusion techniques. Moreover, we explicitly discuss ethical challenges and data governance issues, emphasizing data transparency and animal welfare concerns within precision livestock contexts. We clarify how these methodological innovations directly impact practical farming by enhancing monitoring precision, herd management, and welfare outcomes. Ultimately, this synthesis advocates for strategic, empathetic, and ethically responsible precision dairy farming practices, significantly advancing both dairy cow welfare and operational effectiveness. Full article

Ritual slaughter—understood as the killing of animals without prior stunning for religious purposes—constitutes a legally and ethically intricate domain, situated at the intersection of animal welfare, freedom of religion, public health, and consumer protection. This review offers a critical examination of the influence exerted by international and supranational jurisprudence—most notably the case law of the Court of Justice of the European Union—on the regulatory landscape governing ritual slaughter. While the right to religious freedom enjoys robust protection under European constitutional and human rights frameworks, recent judicial decisions have affirmed the legitimacy of national legislative measures mandating pre-slaughter stunning, insofar as such measures pursue objectives of animal welfare and transparency in the public interest. Particular attention is devoted to seminal rulings originating in Belgium and within the broader EU context, with a focus on the application of the principle of proportionality as a legal mechanism for balancing colliding fundamental rights. The analysis further engages with the scientific and ethical discourse surrounding animal suffering and the legal obligations tied to consumer information and labeling. Taken together, these developments reveal an emergent trajectory within EU law toward the progressive tightening of regulatory standards governing ritual slaughter, shaped by an evolving jurisprudential understanding of animal welfare imperatives. Full article

Rural agricultural markets face unique challenges, yet neuromarketing applications in this sector are understudied. This systematic review investigates how neuroscience has been applied to shape marketing narratives for rural agricultural producers. The objectives were to catalog relevant studies, identify key themes using inductive thematic synthesis, and derive practical implications for rural marketing strategy and future research. We systematically searched English-language, peer-reviewed studies published between 2016 and 2024 across multiple academic databases, following PRISMA guidelines. Two independent reviewers screened the records, resulting in the inclusion of 20 studies. Key data from each study were extracted and synthesized using an inductive thematic analysis approach. The synthesis revealed several recurrent findings. First, in terms of social and community context, farmers showed greater trust and engagement with familiar local buyers than with distant corporations, indicating that local relationships strongly influence producer behavior. Second, regarding product and narrative attributes, marketing narratives that emphasized local provenance, organic or sustainable production, and ethical values such as animal welfare and environmental sustainability resonated strongly with rural consumers. Third, sensory and emotional cues particularly visual elements and storytelling techniques including color, imagery, and packaging design consistently enhanced consumer attention and engagement. Overall, these neuroscience-informed themes suggest that marketing narratives emphasizing authenticity, trust-building, and community values can effectively strengthen rural agricultural marketing. This review provides neuroscience-informed interpretations of key rural marketing challenges, drawing on dual-process theory and consumer decision models for applying neuromarketing insights in this context. Practically, rural producers can leverage these findings by designing marketing messages and packaging that highlight local identity and ethical values, thereby building consumer trust and loyalty. The review also highlights gaps such as the need for more field-based neuromarketing studies and suggests directions for future research, offering guidance for both scholars and practitioners working at the intersection of neuroscience and rural consumer behavior. Full article