Search Results (617)

Objectives: High-intensity interval training (HIIT) has become an increasingly popular conditioning strategy in women’s basketball due to its effectiveness in improving physical performance. The aim of this study was to examine the effects of a six-week HIIT on combined aerobic and anaerobic capacity, running speed, agility, repeated sprint ability, and explosive power in elite female basketball players. Methods: Thirty elite female basketball players (21.81 ± 2.12 years) were randomly assigned to either a HIIT group (n = 15) or a control group (n = 15). Results: Pre- and post-intervention assessments included running speed (0–20 m); the 5–0–5, Zig-zag, and Pro-agility tests; vertical jump performance (CMJ, CMJA, and SJ); repeated sprint ability (RSA); aerobic capacity assessed by the 30–15 Intermittent Fitness Test; and VO₂max. Statistically significant Group × Time interaction effects emerged for running speed (5 m, 10 m, and 20 m), aerobic capacity (30–15 IFT and VO₂max), and Zig-zag agility performance (p < 0.05), indicating greater improvements in the HIIT group compared with the control group. Significant main effects of time were observed for RSA and vertical jump performance, indicating improvements in both groups; however, no significant Group × Time interactions were found. Conclusions: These findings suggest that a six-week HIIT is an effective and time-efficient conditioning strategy for enhancing aerobic capacity, sprint performance, and change-of-direction ability in elite female basketball players. Full article

The vaginal microbiome is often interpreted through static taxonomic patterns. Yet microbial composition alone does not explain why some communities resist perturbation, recover after disruption, or transition toward dysbiosis. This narrative review synthesizes evidence that vaginal microbiome stability is shaped by endocrine phase, epithelial substrate availability, microbial functional capacity, mucosal tone and candidate host modifiers. High-estrogen states, particularly pregnancy, are associated with epithelial maturation, glycogen accumulation, low vaginal pH, and Lactobacillus-dominant communities, whereas postpartum, lactational, menopausal, and other hypoestrogenic states are associated with reduced epithelial support and increased vulnerability to diverse anaerobe-rich configurations. We review the linking of the estrogen–glycogen–Lactobacillus axis, focusing on microbial functions involved in glycogen degradation, lactate production and biofilm persistence, and host pathways that may modify mucosal responsiveness. Direct human genotype-to-vaginal-microbiome stability evidence remains limited; therefore, host genetic features are treated as candidate modifiers rather than validated clinical predictors. We propose a conceptual multi-omic hierarchy for organizing endocrine, epithelial, microbial, immune, temporal, and candidate host-modifier domains relevant to vaginal microbiome resilience. This framework is hypothesis-generating and requires longitudinal, phase-resolved human validation before quantitative prediction or clinical application. Full article

Object: Postoperative infection is a severe complication after pediatric cardiac surgery, which is closely associated with sepsis, multiple organ dysfunction, prolonged mechanical ventilation, extended ICU stay and increased mortality. Conventional microbiological tests (CMT) are limited by low sensitivity, long turnaround time, and poor capacity for detecting viruses and polymicrobial infections. This study aimed to compare the diagnostic efficacy of mNGS with that of CMT, and to explore the impact of polymicrobial infection on clinical outcomes in this high-risk pediatric population. Methods: A retrospective cohort study was conducted on 4889 pediatric patients admitted to the PICU after cardiac surgery from January 2025 to March 2026. A total of 510 patients were diagnosed with postoperative infections, including 879 CMT specimens and 86 mNGS specimens enrolled for analysis. Pathogen detection rates, pathogen spectrum and antimicrobial resistance profiles were compared between the two detection methods. Clinical prognostic indicators including mechanical ventilation duration, PICU length of stay and the requirement for continuous renal replacement therapy (CRRT) were further compared between patients with polymicrobial infection and monomicrobial infection. Results: Respiratory tract infection accounted for 87.8% of all postoperative infections, and Gram-negative bacteria were the predominant pathogens, accounting for 65.9%. The overall pathogen detection rate of mNGS was significantly higher than that of CMT (79.1% vs. 56.5%, p < 0.001). Notably, mNGS exhibited significantly better performance in detecting viruses (37.2% vs. 5.8%, p < 0.001), anaerobic pathogens and polymicrobial infections (38.2% vs. 5.4%, p < 0.001). Patients with polymicrobial infections had significantly longer mechanical ventilation time, longer PICU stay, and higher CRRT utilization rate (all p < 0.05), indicating a poorer clinical prognosis. Gram-negative bacteria showed high resistance to penicillins and early-generation cephalosporins, but remained susceptible to carbapenems and β-lactamase inhibitor combination agents. Gram-positive bacteria showed a high resistance rate to penicillin, while maintaining 100% susceptibility to vancomycin and linezolid. Conclusions: mNGS serves as a more sensitive and comprehensive tool for pathogen detection in children with post-cardiac surgery infections, especially for viral and polymicrobial infections. Polymicrobial infection is an independent risk factor for adverse clinical outcomes. Routine application of mNGS in critically ill children may help guide targeted antimicrobial therapy and improve prognosis. Full article

The growing demand for more sustainable energy alternatives has increased interest in microalgae-based fuels, promising options due to their high biomass productivity, carbon dioxide assimilation capacity, and potential for cultivation using wastewater. However, despite this strong theoretical basis, the industrial consolidation of these routes remains limited, revealing a persistent gap between scientific progress and the technological maturity achieved across specific biofuel pathways. This review examines the current state of the main microalgae-to-fuel conversion routes, emphasizing their technological readiness, proximity to industrial application, and the factors underlying their uneven progress. It also discusses the main opportunities and challenges associated with these biofuels, including cultivation performance, photobioreactor limitations, process intensification, scale-up, integration with waste streams, and downstream processing and product recovery requirements. Overall, this review identifies three main insights. First, the industrial viability of microalgae-based fuels depends less on maximizing individual conversion yields than on overcoming systemic bottlenecks across the production chain. Second, wet biomass conversion routes, particularly hydrothermal liquefaction and anaerobic digestion, appear more compatible with current industrial constraints. Third, lipid- and carbohydrate-based fuels remain more limited by biomass production costs and downstream processing requirements. Accordingly, further progress will depend on process intensification, integrated biorefineries, and technological innovations capable of simultaneously improving productivity, economic viability, and environmental performance. Full article

Objectives: Effective operational functioning within the tactical domain requires a high integration of physical and psychological capacities under stressful conditions. This study investigated the impact of physical exertion at varying intensities (anaerobic and aerobic running) on subsequent change-of-direction speed (CODS) performance among police students, while evaluating the role of mental toughness (MT) and biological sex. Methods: Thirty police students (36.7% female) completed running protocols at different intensities (300-yard shuttle run and 2.4 km Cooper test), immediately followed by the Illinois Agility Test (IAT) to assess CODS performance. Mental toughness was evaluated using the Mental Toughness Index (MTI). Repeated-measures ANOVA and ANCOVA were used to analyze the main and interaction effects. Results: Initial repeated-measures ANOVA revealed that increasing running intensities significantly degraded CODS performance, demonstrating large main effects for both the anaerobic (F = 56.70, p < 0.001, η²_p = 0.661) and aerobic (F = 47.50, p < 0.001, η²_p = 0.621) protocols. After introducing mean-centered MT as a covariate, the main effect of Running Speed remained highly significant in both the anaerobic (F = 57.52, p < 0.001, η²_p = 0.673) and aerobic (F = 46.01, p < 0.001, η²_p = 0.622) models, with no significant univariate interaction effects involving MT or sex on the rate of decline. Mixed ANCOVA showed a significant main effect of sex on absolute IAT times under both anaerobic and aerobic conditions, with males consistently outperforming females. No significant differences in baseline MT scores were observed between sexes (p = 0.507). Conclusions: The findings demonstrate that physical fatigue robustly impairs CODS performance at a parallel rate for both male and female police students. Mental toughness does not neutralize the physiological rate of performance decline under acute fatigue. Practical training programs should integrate physical and psychological conditioning scenarios, while workload management protocols can be uniformly applied across biological sexes. Full article

Storage of livestock effluents represents a major source of ammonia (NH₃) emissions into the atmosphere. Reducing ammonia volatilisation is essential to improve the efficiency of nutrient use, mitigate air pollution, and enhance the overall sustainability of livestock production systems aligned with the principles of the circular economy. Therefore, identifying efficient and sustainable mitigation strategies is crucial. Conventional floating covers are commonly used to reduce emissions; however, they present limitations in terms of management, durability, and cost. This study proposes a novel approach by comparing traditional floating cover materials like straw and light expanded clay with biochar as an innovative and sustainable mitigation strategy to reduce ammonia volatilisation from the liquid fraction of buffalo digestate obtained from an anaerobic digestion plant in southern Italy. All cover materials were applied at a uniform thickness of 2 cm under laboratory conditions using a dynamic chamber technique. Additionally, a cost analysis was performed considering the material purchase cost for an average storage tank of 700 m² and two hypothetical reduction efficiencies (50% and 70%). Results indicated that biochar was the most effective cover, achieving a 67% reduction in ammonia emissions compared with the uncovered control. Light expanded clay exhibited the lowest efficiency, likely due to its insufficient sealing capacity at the applied thickness. From an economic perspective, biochar becomes increasingly competitive when emission reduction efficiency is accounted for, owing to its favourable physical–chemical properties. The results highlight the potential of using biochar as a sustainable, circular strategy for mitigating atmospheric emissions and improving nitrogen conservation, while also creating opportunities for its subsequent reuse in agriculture as a soil amendment. Full article

The article presents an energy, economic and environmental (3-E) analysis of a reference wastewater treatment plant (WWTP) with a capacity of 200,000 population equivalent (PE). The analysis includes sewage sludge treatment, anaerobic digestion (AD), combined heat and power (CHP), and mono-incineration of solar-dried sludge. The specific investment cost for the reference WWTP is 435 €/PE. Annual costs for operation and maintenance are estimated at 26 €/(PE·y) and the energy costs are 5 €/(PE·y). The annual energy demands are 32 kWh_el/(PE·y) of electricity and 14 kWh_th/(PE·y) of thermal energy for digesters’ heating. For a specific sludge quantity of 20 kg_DS/(PE·year), the biogas production is 245 Nm³/t_DS or 5 m³/(PE·y). Biogas-driven CHP supplies 10.3 kWh/(PE·year) of electricity and 14.7 kWh/(PE·year) of thermal energy, which meets 30% of the electrical demand and 100% of the thermal energy demand. Total (capital and operation) costs of sludge mono-incineration are evaluated at 300 €/t_DM or 6 €/PE. The heating value of digested and solar-dried sludge is 2 kWh/kg_WM. The total cost of the solar drying system is 30 €/PE while the sludge solar drying rate is 370 kg_DM/(m²·y). The environmental analysis showed that the on-site carbon footprint of the reference WWTP is 50 kg_CO2eq/(PE·y), with the largest contributions arising from N₂O emissions during wastewater treatment, CO₂ from sludge mono-incineration, and CO₂ from biogas combustion in the CHP unit. Full article

Background: Artemisia absinthium (A. absinthium) is a perennial plant valued for its antibacterial, antioxidant, and anti-inflammatory properties, exhibiting broader therapeutic potential. Given the need to deliver low doses of A. absinthium extract, mesoporous silica nanoparticles have attracted considerable attention as promising nanocarriers due to their distinctive physical and chemical properties. Methods: Physicochemical characterization of the materials was performed and biological assays were conducted to investigate the ROS, antibacterial and antioxidant activity of A. absinthium extract encapsulated within cerium- and calcium-doped mesoporous silica nanoparticles (MNSiCaCe) against both aerobic and anaerobic bacteria. Results: FTIR, SEM, and BET analysis confirmed successful synthesis of the MNSiCaCe. Phytochemical profiling of Artemisia absinthium extract using HPLC revealed the presence of artemisinin and a rich composition of phenolic and flavonoid constituents, with a total phenolic content of 182 ± 3.6 mg GAE/100 g dry plant material and a total flavonoid content of 42.5 ± 0.6 mg QE/100 g. Quantitative drug loading profiling demonstrated that while plain MNSi nanocarriers achieved a loading capacity of 16.96%, the MNSiCaCe enhanced this threshold to 43.11%. The in vitro controlled-release kinetics exhibited a highly prolonged and slow-release profile of the MNSiCaCe. The materials demonstrated excellent hemocompatibility and high mitochondrial activity with human periodontal ligament cells (hPDLCs). Elevated ROS generation was observed under conditions where antibacterial activity was most pronounced. While the artemisinin-doped nanoparticles showed notable antibacterial effects, the complete Artemisia absinthium-loaded nanoparticles achieved a significantly greater reduction in bacterial viability probably due to the synergistic interaction between artemisinin and the extract’s rich polyphenol profile. Conclusions: These findings highlight MNSiCaCe as a promising and safe nanocarrier system for drug delivery, with strong antibacterial potential, offering valuable applications in antibacterial therapies. Full article

This study investigated possibilities to increase the efficiency of anaerobic digestion of sewage sludge using biochar produced by pyrolysis of digested sludge (sludgechar). Experiments were conducted in continuous laboratory bioreactors operated at the same loading rate, gradually increased from 3.2 to 4.5 g/(L·d) (COD) under mesophilic conditions (40 °C). Sludgechar (SCH) was dosed into the experimental bioreactor at a rate of 0.4–1.3 g/(L·d), corresponding to 12–28% of the added COD. Biogas production in the experimental bioreactor increased by 6.9–33% compared with the control bioreactor, while the CH₄ concentration remained comparable, averaging 62.8%. The COD removal efficiency remained high in the sludgechar-supplemented bioreactor as the loading rate increased, whereas it decreased in the control bioreactor, corresponding to lower biogas production. The adsorption capacity, alkalinity, and mineral buffering properties of sludgechar prevented pH decline and the accumulation of volatile fatty acids (VFAs) at higher substrate loading. The pH values were less affected by increasing organic loading in the experimental than in the control bioreactor and remained within 6.9–7.0. Continuous experiments confirmed that sludgechar can facilitate stable operation at loading rates that would otherwise cause process failure. However, the low carbon-to-inorganic ratio of sludgechar is its significant disadvantage. Full article

Objectives: Local positioning systems (LPSs) used in indoor team sports generate a large number of external load variables, often exceeding practical monitoring capacity. The redundancy and overlap among these variables make it difficult to identify the most informative metrics for performance analysis and load management. This study aimed to reduce the dimensionality of external load variables derived from LPS data and to identify data-driven external-load observation profiles using principal component analysis and clustering techniques. Methods: A total of 188 observations from indoor team sports (basketball, handball, and futsal) were analyzed. Continuous external load variables were standardized and subjected to principal component analysis (PCA), with component retention based on a ≥90% cumulative explained variance threshold. K-means clustering was applied in both the full standardized feature space and the PCA-reduced space. The optimal number of clusters was determined using silhouette analysis and the elbow method. Agreement between clustering solutions was assessed using Adjusted Rand Index (ARI) and Normalized Mutual Information (NMI). Cluster characteristics were further examined using descriptive statistics and variable separation analysis. Results: The first two principal components explained 53.7% of the total variance, representing high-intensity external load and neuromuscular load dimensions, while 12 components were required to exceed 90% cumulative explained variance. Clustering analysis consistently identified three moderately separated clusters in both the full and PCA-reduced spaces. The PCA-based solution demonstrated improved separation (silhouette = 0.362) compared to the full-space solution (silhouette = 0.319). Agreement between clustering approaches was high (ARI = 0.981; NMI = 0.971), indicating that dimensionality reduction largely preserved the main clustering structure within the analyzed dataset. The most discriminative variables included jump load, acceleration load, metabolic power, and anaerobic activity distance. Conclusions: A large set of external load variables can be reduced into interpretable latent dimensions that support exploratory external-load profile identification. The combination of PCA and clustering provides an exploratory and structure-preserving framework for summarizing complex external-load datasets and identifying latent load dimensions. These findings may assist future monitoring strategies; however, the practical utility of the identified profiles requires prospective validation before implementation in training-load management. Full article

The transition to renewable energy requires scalable and sustainable hydrogen production technologies. Dark fermentation (DF) can generate biohydrogen from diverse biomass feedstock, but its efficiency remains limited. Immobilising anaerobic consortia offers a route to improve performance. This study reports on the immobilisation of whole cells in hybrid biochar–alginate beads (BAB) compared with control alginate beads (CAB) during DF. Biochar from oakwood and water hyacinth, pyrolysed at 450 and 600/650 °C, were incorporated into BAB. BAB increased biohydrogen production rates by 1.4–2.6-fold relative to CAB, driven by enhanced microbial attachment, synergistic interactions, and improved mass transfer. High-temperature biochar generated the strongest effects, raising hydrogen yield by up to 23% and shortening the lag phase by 94%. Biochar properties, including porosity, surface area, inorganic content, electrical conductivity and buffering capacity, likely support these effects. These results establish hybrid biochar-alginate support as a promising platform to accelerate DF and advance biohydrogen as a sustainable biofuel. Full article

Anaerobic co-digestion (AcoD) is increasingly applied in sewage-sludge management and organic-waste treatment because it can improve methane recovery, stabilize mixed substrates, and reduce life-cycle greenhouse-gas emissions under appropriate feedstock and operating conditions. However, existing reviews still focus mainly on feedstock types or isolated enhancement measures and less often connect synergistic mechanisms with engineering implementation and carbon outcomes. The specific novelty of this review is to connect functional feedstock classification, mechanism boundaries, engineering controls, and carbon-performance evaluation within one sludge-centered AcoD framework. This review synthesizes recent progress in AcoD of sewage sludge, food waste, livestock manure, crop residues, and industrial organic streams through a chain from feedstock traits to substrate interactions, microbial responses, engineering performance, and carbon benefits. Feedstocks are reorganized by function rather than by waste name, highlighting how carbon-to-nitrogen contrast, buffering capacity, hydrolysis recalcitrance, and inhibitor profiles jointly define synergy potential. Key mechanisms, including C/N balancing, hydrolysis complementarity, inhibitor mitigation, and direct interspecies electron transfer (DIET), are discussed together with their applicability limits. Representative evidence shows methane-yield or methane-production increases of about 41–55% for selected food-waste–manure blends, approximately 45% for rice–straw–pig manure systems after cellulolytic pretreatment, and approximately 16–55% for selected additive strategies; these values are illustrative rather than directly comparable because the underlying studies differ in substrates, baselines, reactor configurations, pretreatment conditions, and operating parameters. The review then translates mechanism into practice through pretreatment, reactor-selection templates, operating windows, additive reinforcement, and artificial-intelligence-assisted monitoring. Representative cases and life-cycle evidence indicate that AcoD can improve methane productivity while lowering greenhouse-gas emissions relative to landfill or mono-digestion pathways when energy substitution and nutrient recycling are credibly counted. Remaining bottlenecks include incomplete kinetic integration, limited DIET quantification, insufficient reporting of quantitative operating ranges and additive dosages, and weak coupling of carbon, economics, and regional feedstock dynamics. The revised review therefore treats AcoD as a sludge-centered mechanism-to-engineering framework and highlights two transferability gaps that require stronger standardization: biodegradation/toxicity testing and local co-substrate logistics. Full article

Background: New Zealand blackcurrant (NZBC) is an anthocyanin-rich supplement with reported ergogenic effects in endurance exercise; however, its effects in resistance-trained adults remain largely unexplored. Objective: This study aimed to examine whether seven days of low- or high-dose NZBC supplementation improves resistance exercise performance, anaerobic capacity, and cognitive function in resistance-trained adults. Methods: Twenty resistance-trained adults completed a randomized, double-blind, placebo-controlled crossover trial with four conditions: no-capsule control (CON), placebo (PL), low-dose blackcurrant (LDBC; 250 mg·day⁻¹), and high-dose blackcurrant (HDBC; 600 mg·day⁻¹), each for seven days. Outcomes included bench press and leg press 1RM, total lifting volume, Tendo-derived bench press power, 30 s Wingate performance, Stroop Color–Word Test scores, readiness, perceived exertion, hemodynamic responses, and adverse events. Results: LDBC and HDBC increased bench press 1RM versus CON and PL, with increases versus CON of +3.33 kg (ES = 0.72; p = 0.005) and +2.34 kg (ES = 0.49; p = 0.041), respectively. Leg press 1RM was higher in PL, LDBC, and HDBC versus CON, with the largest effects observed for LDBC (+37.2 kg, ES = 1.33; p < 0.001) and HDBC (+25.8 kg, ES = 1.11; p < 0.001). Leg press total lifting volume was substantially higher with LDBC (+2627 kg, ES = 1.56; p < 0.001) and HDBC (+1025 kg, ES = 0.74; p = 0.004) versus CON. Bench press volume showed no significant overall treatment effect (p > 0.05). For Tendo-derived power, HDBC exceeded PL for peak (+79.5 W; p = 0.006) and mean power (+46.2 W; p = 0.026). Wingate outcomes did not differ across conditions (all p > 0.05). LDBC exceeded PL on Stroop Color, Color–Word, and total scores (all p < 0.05); HDBC exceeded PL on Color–Word only. Hemodynamic responses and adverse events were comparable across all conditions. Conclusions: Short-term NZBC supplementation improved selected resistance-exercise and cognitive outcomes, with the strongest evidence observed for outcomes that exceeded both CON and PL. The PL response relative to CON suggests that non-specific capsule, expectancy, repeated testing, or period effects may have contributed to some of the lower-body improvements; therefore, placebo-controlled contrasts should be emphasized when interpreting NZBC-specific efficacy. Wingate performance was unaffected, and both doses were well tolerated over the short-term supplementation period. Full article

Circular agriculture has emerged as a promising framework for addressing the inefficiencies and environmental pressures associated with conventional food production systems. Within this context, livestock systems can play a transformative role by enabling waste valorisation, enhancing nutrient recycling, and improving overall resource-use efficiency. This review critically examines the multifunctional role of livestock in circular agriculture, with a particular focus on their capacity to convert non-human-edible biomass, such as crop residues, agro-industrial by-products, and food waste, into high-value animal-sourced foods. Drawing on the recent literature, the analysis explores how livestock systems can be reconfigured to utilise non-human-edible biomass, including crop residues, agro-industrial by-products, and food waste, thereby reducing competition between feed and food while enhancing sustainability outcomes. The findings highlight that livestock can function as biological upcycles, converting low-value materials into high-quality animal products, while also contributing to closed nutrient loops through manure management and integration with crop production. Additional benefits include the generation of renewable energy through anaerobic digestion and improved economic resilience through diversified outputs. However, the extent of these benefits depends on system design, management practices, and regional context. Despite their potential, circular livestock systems face challenges related to greenhouse gas emissions, regulatory constraints, economic feasibility, and knowledge gaps. These challenges highlight the need for a systems-based evaluation that accounts for environmental, economic, and social dimensions. The study concludes that livestock can contribute meaningfully to sustainable food system transitions when aligned with circular principles, but their role must be critically assessed to avoid burden-shifting and unintended environmental impacts. Full article

Background: Optimizing physical performance in youth football requires a comprehensive understanding of the interplay among behavioural factors, structural body composition, and functional cardiorespiratory capacity. While sports nutrition knowledge is hypothesized to influence athletic development, its concurrent relationships with regional body compartments and objective cardiopulmonary exercise testing (CPET) metrics remain poorly characterized in adolescent athletes. Methods: A cross-sectional study approach analysed body composition via bioelectrical impedance analysis (BIA), maximal cardiorespiratory testing, and sports nutrition knowledge evaluation using the Nutrition for Sport Knowledge Questionnaire (NSKQ). Structural associations and functional predictive capacities were analysed. Results: The cohort demonstrated an average VO₂max of 51.18 ± 16.67 mL/kg/min and a mean total nutrition knowledge score of 43.56 ± 18.06 out of 81 (53.8%). Total and domain-specific nutrition knowledge scores were not associated with body mass index (BMI), fat-free mass (FFM), or fat-free mass percentage (FFM%). Higher nutrition knowledge scores were independently associated with superior VO₂max and anaerobic threshold (AT) metrics. Exploratory geographic analyses revealed that rural-residing participants possessed significantly higher cardiorespiratory performance values and greater baseline nutrition knowledge profiles than their urban peers. Conclusions: In adolescent male football players, sports nutrition knowledge was not associated with static body composition measures but showed exploratory positive associations with selected cardiorespiratory fitness markers. These findings should be interpreted as cross-sectional and hypothesis-generating, as some potential confounding mediators were not assessed. These findings suggest that higher sports nutrition literacy may serve as a starting point for performance-supportive behaviours and metabolic conditioning, to some degree, warranting future interventional studies. Full article