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Keywords = agricultural application

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28 pages, 2057 KiB  
Article
Design and Fabrication of a Cost-Effective, Remote-Controlled, Variable-Rate Sprayer Mounted on an Autonomous Tractor, Specifically Integrating Multiple Advanced Technologies for Application in Sugarcane Fields
by Pongpith Tuenpusa, Kiattisak Sangpradit, Mano Suwannakam, Jaturong Langkapin, Alongklod Tanomtong and Grianggai Samseemoung
AgriEngineering 2025, 7(8), 249; https://doi.org/10.3390/agriengineering7080249 - 5 Aug 2025
Abstract
The integration of a real-time image processing system using multiple webcams with a variable rate spraying system mounted on the back of an unmanned tractor presents an effective solution to the labor shortage in agriculture. This research aims to design and fabricate a [...] Read more.
The integration of a real-time image processing system using multiple webcams with a variable rate spraying system mounted on the back of an unmanned tractor presents an effective solution to the labor shortage in agriculture. This research aims to design and fabricate a low-cost, variable-rate, remote-controlled sprayer specifically for use in sugarcane fields. The primary method involves the modification of a 15-horsepower tractor, which will be equipped with a remote-control system to manage both the driving and steering functions. A foldable remote-controlled spraying arm is installed at the rear of the unmanned tractor. The system operates by using a webcam mounted on the spraying arm to capture high-angle images above the sugarcane canopy. These images are recorded and processed, and the data is relayed to the spraying control system. As a result, chemicals can be sprayed on the sugarcane accurately and efficiently based on the insights gained from image processing. Tests were conducted at various nozzle heights of 0.25 m, 0.5 m, and 0.75 m. The average system efficiency was found to be 85.30% at a pressure of 1 bar, with a chemical spraying rate of 36 L per hour and a working capacity of 0.975 hectares per hour. The energy consumption recorded was 0.161 kWh, while fuel consumption was measured at 6.807 L per hour. In conclusion, the development of the remote-controlled variable rate sprayer mounted on an unmanned tractor enables immediate and precise chemical application through remote control. This results in high-precision spraying and uniform distribution, ultimately leading to cost savings, particularly by allowing for adjustments in nozzle height from a minimum of 0.25 m to a maximum of 0.75 m from the target. Full article
(This article belongs to the Special Issue Implementation of Artificial Intelligence in Agriculture)
27 pages, 37457 KiB  
Article
Multi-Sensor Flood Mapping in Urban and Agricultural Landscapes of the Netherlands Using SAR and Optical Data with Random Forest Classifier
by Omer Gokberk Narin, Aliihsan Sekertekin, Caglar Bayik, Filiz Bektas Balcik, Mahmut Arıkan, Fusun Balik Sanli and Saygin Abdikan
Remote Sens. 2025, 17(15), 2712; https://doi.org/10.3390/rs17152712 - 5 Aug 2025
Abstract
Floods stand as one of the most harmful natural disasters, which have become more dangerous because of climate change effects on urban structures and agricultural fields. This research presents a comprehensive flood mapping approach that combines multi-sensor satellite data with a machine learning [...] Read more.
Floods stand as one of the most harmful natural disasters, which have become more dangerous because of climate change effects on urban structures and agricultural fields. This research presents a comprehensive flood mapping approach that combines multi-sensor satellite data with a machine learning method to evaluate the July 2021 flood in the Netherlands. The research developed 25 different feature scenarios through the combination of Sentinel-1, Landsat-8, and Radarsat-2 imagery data by using backscattering coefficients together with optical Normalized Difference Water Index (NDWI) and Hue, Saturation, and Value (HSV) images and Synthetic Aperture Radar (SAR)-derived Grey Level Co-occurrence Matrix (GLCM) texture features. The Random Forest (RF) classifier was optimized before its application based on two different flood-prone regions, which included Zutphen’s urban area and Heijen’s agricultural land. Results demonstrated that the multi-sensor fusion scenarios (S18, S20, and S25) achieved the highest classification performance, with overall accuracy reaching 96.4% (Kappa = 0.906–0.949) in Zutphen and 87.5% (Kappa = 0.754–0.833) in Heijen. For the flood class F1 scores of all scenarios, they varied from 0.742 to 0.969 in Zutphen and from 0.626 to 0.969 in Heijen. Eventually, the addition of SAR texture metrics enhanced flood boundary identification throughout both urban and agricultural settings. Radarsat-2 provided limited benefits to the overall results, since Sentinel-1 and Landsat-8 data proved more effective despite being freely available. This study demonstrates that using SAR and optical features together with texture information creates a powerful and expandable flood mapping system, and RF classification performs well in diverse landscape settings. Full article
(This article belongs to the Special Issue Remote Sensing Applications in Flood Forecasting and Monitoring)
17 pages, 1416 KiB  
Article
Humic Substances Promote the Activity of Enzymes Related to Plant Resistance
by Rakiely M. Silva, Fábio L. Olivares, Lázaro E. P. Peres, Etelvino H. Novotny and Luciano P. Canellas
Agriculture 2025, 15(15), 1688; https://doi.org/10.3390/agriculture15151688 - 5 Aug 2025
Abstract
The extensive use of pesticides has significant implications for public health and the environment. Breeding crop plants is the most effective and environmentally friendly approach to improve the plants’ resistance. However, it is time-consuming and costly, and it is sometimes difficult to achieve [...] Read more.
The extensive use of pesticides has significant implications for public health and the environment. Breeding crop plants is the most effective and environmentally friendly approach to improve the plants’ resistance. However, it is time-consuming and costly, and it is sometimes difficult to achieve satisfactory results. Plants induce defense responses to natural elicitors by interpreting multiple genes that encode proteins, including enzymes, secondary metabolites, and pathogenesis-related (PR) proteins. These responses characterize systemic acquired resistance. Humic substances trigger positive local and systemic physiological responses through a complex network of hormone-like signaling pathways and can be used to induce biotic and abiotic stress resistance. This study aimed to assess the effect of humic substances on the activity of phenylalanine ammonia-lyase (PAL), peroxidase (POX), and β-1,3-glucanase (GLU) used as a resistance marker in various plant species, including orange, coffee, sugarcane, soybeans, maize, and tomato. Seedlings were treated with a dilute aqueous suspension of humic substances (4 mM C L−1) as a foliar spray or left untreated (control). Leaf tissues were collected for enzyme assessment two days later. Humic substances significantly promoted the systemic acquired resistance marker activities compared to the control in all independent assays. Overall, all enzymes studied in this work, PAL, GLUC, and POX, showed an increase in activity by 133%, 181%, and 149%, respectively. Among the crops studied, citrus and coffee achieved the highest activity increase in all enzymes, except for POX in coffee, which showed a decrease of 29% compared to the control. GLUC exhibited the highest response to HS treatment, the enzyme most prominently involved in increasing enzymatic activity in all crops. Plants can improve their resistance to pathogens through the exogenous application of HSs as this promotes the activity of enzymes related to plant resistance. Finally, we consider the potential use of humic substances as a natural chemical priming agent to boost plant resistance in agriculture Full article
(This article belongs to the Special Issue Biocontrol Agents for Plant Pest Management)
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18 pages, 2357 KiB  
Article
Nitrogen Fertilizer Reduction in Rice–Eel Co-Culture System Improves the Soil Microbial Diversity and Its Functional Stability
by Mengqian Ma, Weiguang Lv, Yu Huang, Juanqin Zhang, Shuangxi Li, Naling Bai, Haiyun Zhang, Xianpu Zhu, Chenglong Xu and Hanlin Zhang
Plants 2025, 14(15), 2425; https://doi.org/10.3390/plants14152425 - 5 Aug 2025
Abstract
The ecological rice–eel co-culture system is not only beneficial for enhancing productivity and sustainability in agriculture but also plays a crucial role in promoting environmental health. In the present study, based on the long-term positioning trial of the rice–eel co-culture system that began [...] Read more.
The ecological rice–eel co-culture system is not only beneficial for enhancing productivity and sustainability in agriculture but also plays a crucial role in promoting environmental health. In the present study, based on the long-term positioning trial of the rice–eel co-culture system that began in 2016 and was sampled in 2023, the effects of reduced nitrogen fertilizer application on soil physico-chemical properties and the bacterial community were investigated. Treatments included a conventional regular fertilization treatment (RT), rice–eel co-culture system regular fertilization (IT), and nitrogen-reduction 10%, 30%, and 50% fertilization treatments (IT90, IT70, and IT50). Our research demonstrated the following: (1) Compared to RT, IT significantly increased soil water-stable macroaggregates (R0.25), mean weight diameter (MWD), geometric mean diameter (GMD), and available phosphorus content, with the increases of 15.66%, 25.49%, 36.00%, and 18.42%, respectively. Among the nitrogen-reduction fertilization treatments, IT90 showed the most significant effect. Compared to IT, IT90 significantly increased R0.25, MWD, GMD, and available nitrogen content, with increases of 4.4%, 7.81%, 8.82%, and 28.89%, respectively. (2) Compared to RT, at the phylum level, the diversity of Chloroflexi was significantly increased under IT and IT50, and the diversity of Gemmatimonadota was significantly increased under IT90, IT70, and IT50. The diversity of Acidobacteriota was significantly higher in IT90 and IT70 compared to IT. It was shown that the rice–eel co-culture system and nitrogen fertilizer reduction could effectively improve the degradation capacity of organic matter and promote soil nitrogen cycling. In addition, redundancy analysis (RDA) identified total phosphorus, total nitrogen, and available nitrogen (p = 0.007) as the three most important environmental factors driving changes in the bacterial community. (3) The functional prediction analysis of soil microbiota showed that, compared to RT, the diversity of pathways related to biosynthesis (carbohydrate biosynthesis and cell structure biosynthesis) and metabolism (L-glutamate and L-glutamine biosynthesis) was significantly higher under IT70, IT90, IT, and IT50 (in descending order). However, the diversity of pathways associated with degradation/utilization/assimilation (secondary metabolite degradation and amine and polyamine degradation) was significantly lower under all the rice–eel co-culture treatments. In conclusion, the rice–eel co-culture system improved soil physicochemical properties and the soil microbial environment compared with conventional planting, and the best soil improvement was achieved with 10% less N fertilizer application. Full article
(This article belongs to the Special Issue Chemical Properties of Soils and its Impact on Plant Growth)
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21 pages, 3832 KiB  
Article
Effects of Water Use Efficiency Combined with Advancements in Nitrogen and Soil Water Management for Sustainable Agriculture in the Loess Plateau, China
by Hafeez Noor, Fida Noor, Zhiqiang Gao, Majed Alotaibi and Mahmoud F. Seleiman
Water 2025, 17(15), 2329; https://doi.org/10.3390/w17152329 - 5 Aug 2025
Abstract
In China’s Loess Plateau, sustainable agricultural end products are affected by an insufficiency of water resources. Rising crop water use efficiency (WUE) through field management pattern improvement is a crucial plan of action to address this issue. However, there is no agreement among [...] Read more.
In China’s Loess Plateau, sustainable agricultural end products are affected by an insufficiency of water resources. Rising crop water use efficiency (WUE) through field management pattern improvement is a crucial plan of action to address this issue. However, there is no agreement among researchers on the most appropriate field management practices regarding WUE, which requires further integrated quantitative analysis. We conducted a meta-analysis by quantifying the effect of agricultural practices surrounding nitrogen (N) fertilizer management. The two experimental cultivars were Yunhan–20410 and Yunhan–618. The subplots included nitrogen 0 kg·ha−1 (N0), 90 kg·ha−1 (N90), 180 kg·ha−1 (N180), 210 kg·ha−1 (N210), and 240 kg·ha−1 (N240). Our results show that higher N rates (up to N210) enhanced water consumption during the node-flowering and flowering-maturity time periods. YH–618 showed higher water use during the sowing–greening and node-flowering periods but decreased use during the greening-node and flowering-maturity periods compared to YH–20410. The N210 treatment under YH–618 maximized water use efficiency (WUE). Increased N rates (N180–N210) decreased covering temperatures (Tmax, Tmin, Taver) during flowering, increasing the level of grain filling. Spike numbers rose with N application, with an off-peak at N210 for strong-gluten wheat. The 1000-grain weight was at first enhanced but decreased at the far end of N180–N210. YH–618 with N210 achieved a harvest index (HI) similar to that of YH–20410 with N180, while excessive N (N240) or water reduced the HI. Dry matter accumulation increased up to N210, resulting in earlier stabilization. Soil water consumption from wintering to jointing was strongly correlated with pre-flowering dry matter biological process and yield, while jointing–flowering water use was linked to post-flowering dry matter and spike numbers. Post-flowering dry matter accumulation was critical for yield, whereas spike numbers positively impacted yield but negatively affected 1000-grain weight. In conclusion, our results provide evidence for determining suitable integrated agricultural establishment strategies to ensure efficient water use and sustainable production in the Loess Plateau region. Full article
(This article belongs to the Special Issue Soil–Water Interaction and Management)
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21 pages, 9265 KiB  
Article
Towards a Sustainable Process of Conservation/Reuse of Built Cultural Heritage: A “Coevolutionary” Approach to Circular Economy in the Case of the Decommissioned Industrial Agricultural Consortium in the Corbetta, Metropolitan Area of Milan, Italy
by Mehrnaz Rajabi, Stefano Della Torre and Arian Heidari Afshari
Land 2025, 14(8), 1595; https://doi.org/10.3390/land14081595 - 5 Aug 2025
Abstract
This paper aims to explore the potentialities and systemic relationships between the ‘regenerative’ process and ‘circular economy’ concept within the conservation and reuse of a built cultural heritage framework through contextualizing the concept of ‘process programming’ of the Preventive and Planned Conservation methodology. [...] Read more.
This paper aims to explore the potentialities and systemic relationships between the ‘regenerative’ process and ‘circular economy’ concept within the conservation and reuse of a built cultural heritage framework through contextualizing the concept of ‘process programming’ of the Preventive and Planned Conservation methodology. As a case study, it depicts a decommissioned industrial agricultural silo in Corbetta—a small historic city with its hinterland located in the protected Southern Milan Regional Agricultural Park. The context includes the industrial agricultural lands of the 20th century, together with historical water infrastructure, farmhouses, and the typical flora of the Lombardy region, all evidences of Corbetta’s rural archaeological values and the sophisticated material culture of its past collective production/economy system—the locus in which the silo was once one of the main productive symbols of Corbetta’s agricultural identity. Within such a complex and challenging context, this paper argues in favor of the constructive role of such a methodology in upholding circular economy principles within the process of conservation and reuse of the silo, highlighting its broader application of the ‘coevolution’ concept from a multidisciplinary long-term perspective. Full article
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13 pages, 223 KiB  
Article
Preliminary Research on the Efficacy of Selected Herbicides Approved for Use in Sustainable Agriculture Using Spring Cereals as an Example
by Piotr Szulc, Justyna Bauza-Kaszewska, Marek Selwet and Katarzyna Ambroży-Deręgowska
Sustainability 2025, 17(15), 7090; https://doi.org/10.3390/su17157090 (registering DOI) - 5 Aug 2025
Abstract
The objective of this study was to evaluate the efficacy of selected herbicides permitted for use in sustainable agriculture, specifically targeting spring rye and spring barley in a no-till farming system. The application of chemical herbicide protection in the cultivation of spring rye [...] Read more.
The objective of this study was to evaluate the efficacy of selected herbicides permitted for use in sustainable agriculture, specifically targeting spring rye and spring barley in a no-till farming system. The application of chemical herbicide protection in the cultivation of spring rye and barley significantly increased the yield and improved the quality parameters of the harvested grain, with the most pronounced effect observed in spring barley. The effectiveness of the herbicide treatment in reducing the number of weeds was 99.4% for spring rye and 82.39% for spring barley. The study demonstrated that the application of chemical herbicide protection had a positive impact on the quality parameters of spring barley grain. Both the thousand-grain weight and protein content were significantly higher in the grain collected from protected plots compared to the control plots. By utilizing herbicides permitted for use in integrated production (IP) in a sustainable manner, we protect the environment while minimizing the impact on crop yields and maintaining the quality of the harvested produce. Full article
21 pages, 12523 KiB  
Article
Essential Oils as an Antifungal Alternative for the Control of Various Species of Fungi Isolated from Musa paradisiaca: Part I
by Maritza D. Ruiz Medina and Jenny Ruales
Microorganisms 2025, 13(8), 1827; https://doi.org/10.3390/microorganisms13081827 - 5 Aug 2025
Abstract
This study evaluated the antifungal potential of essential oils (EOs): oregano (Origanum vulgare), rosemary (Salvia rosmarinus), clove (Syzygium aromaticum), thyme (Thymus vulgaris), cinnamon (Cinnamomum verum), and basil (Ocimum basilicum). These oils [...] Read more.
This study evaluated the antifungal potential of essential oils (EOs): oregano (Origanum vulgare), rosemary (Salvia rosmarinus), clove (Syzygium aromaticum), thyme (Thymus vulgaris), cinnamon (Cinnamomum verum), and basil (Ocimum basilicum). These oils were tested against fungi isolated from banana peels (Musa paradisiaca). The fungi tested were identified through macroscopic and microscopic analyses and DNA sequencing, after being isolated in potato dextrose agar (PDA) medium modified with 0.05% chloramphenicol. Subsequently, the antifungal properties of the tested essential oils were evaluated in vitro at concentrations of 200, 400, 600, 800, and 1000 ppm prepared in a 0.05% Tween 80 solution. Cinnamon EOs showed the highest antifungal activity, significantly inhibiting the growth of pathogens at a concentration of 400 ppm. Other EOs showed moderate effects at higher concentrations: rosemary inhibited fungal growth at 600 ppm, oregano at 800 ppm, and clove at 1000 ppm. These findings highlight the potential of EOs as eco-friendly alternatives to synthetic fungicides, contributing to the development of sustainable agricultural practices and the post-harvest management of bananas. It is recommended to conduct future research to assess the economic viability and practical impacts of large-scale applications. Full article
(This article belongs to the Special Issue Current Pattern in Epidemiology and Antifungal Resistance)
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26 pages, 3287 KiB  
Review
Endophytic Species of the Genus Colletotrichum as a Source of Bioactive Metabolites: A Review of Their Biotechnological Potential
by Manuela Vitoria Nascimento da Silva, Andrei da Silva Alexandre and Cecilia Veronica Nunez
Microorganisms 2025, 13(8), 1826; https://doi.org/10.3390/microorganisms13081826 - 5 Aug 2025
Abstract
The genus Colletotrichum is widely known for its phytopathological significance, especially as the causative agent of anthracnose in diverse agricultural crops. However, recent studies have unveiled its ecological versatility and biotechnological potential, particularly among endophytic species. These fungi, which asymptomatically colonize plant tissues, [...] Read more.
The genus Colletotrichum is widely known for its phytopathological significance, especially as the causative agent of anthracnose in diverse agricultural crops. However, recent studies have unveiled its ecological versatility and biotechnological potential, particularly among endophytic species. These fungi, which asymptomatically colonize plant tissues, stand out as high-yielding producers of bioactive secondary metabolites. Given their scientific and economic relevance, this review critically examines endophytic Colletotrichum species, focusing on the chemical diversity and biological activities of the metabolites they produce, including antibacterial, antifungal, and cytotoxic activity against cancer cells, and antioxidant properties. This integrative review was conducted through a structured search of scientific databases, from which 39 relevant studies were selected, highlighting the chemical and functional diversity of these compounds. The analyzed literature emphasizes their potential applications in pharmaceutical, agricultural, and industrial sectors. Collectively, these findings reinforce the promising biotechnological potential of Colletotrichum endophytes not only as sources of bioactive metabolites but also as agents involved in ecological regulation, plant health promotion, and sustainable production systems. Full article
(This article belongs to the Special Issue Endophytic Fungus as Producers of New and/or Bioactive Substances)
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20 pages, 3741 KiB  
Article
Use of Amino Acids and Organic Waste Extracts to Improve the Quality of Liquid Nitrogen–Calcium–Magnesium Fertilizers
by Eglė Didžiulytė and Rasa Šlinkšienė
Sustainability 2025, 17(15), 7081; https://doi.org/10.3390/su17157081 (registering DOI) - 5 Aug 2025
Abstract
Agriculture is one of the most important sectors of the global economy, but it increasingly faces sustainability challenges in meeting rising food demands. The intensive use of mineral fertilizers not only improves yields, but also causes negative environmental impacts such as increasing greenhouse [...] Read more.
Agriculture is one of the most important sectors of the global economy, but it increasingly faces sustainability challenges in meeting rising food demands. The intensive use of mineral fertilizers not only improves yields, but also causes negative environmental impacts such as increasing greenhouse gas emissions, water eutrophication, and soil degradation. To develop more sustainable solutions, the focus is on organic fertilizers, which are produced using waste and biostimulants such as amino acids. The aim of this study was to develop and characterize liquid nitrogen–calcium–magnesium fertilizers produced by decomposing dolomite with nitric acid followed by further processing and to enrich them with a powdered amino acid concentrate Naturamin-WSP and liquid extracts from digestate, a by-product of biogas production. Nutrient-rich extracts were obtained using water and potassium hydroxide solutions, with the latter proving more effective by yielding a higher organic carbon content (4495 ± 0.52 mg/L) and humic substances, which can improve soil structure. The produced fertilizers demonstrated favourable physical properties, including appropriate viscosity and density, as well as low crystallization temperatures (eutectic points from –3 to –34 °C), which are essential for storage and application in cold climates. These properties were achieved by adjusting the content of nitrogenous compounds and bioactive extracts. The results of the study show that liquid fertilizers enriched with organic matter can be an effective and more environmentally friendly alternative to mineral fertilizers, contributing to the development of the circular economy and sustainable agriculture. Full article
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13 pages, 1859 KiB  
Article
Suspension Fertilizers Based on Waste Organic Matter from Peanut Oil Extraction By-Products
by Sainan Xiang, Baoshen Li and Yang Lyu
Agronomy 2025, 15(8), 1885; https://doi.org/10.3390/agronomy15081885 - 5 Aug 2025
Abstract
The use of chemical fertilizers has significantly increased crop yields but has also led to soil problems such as nutrient imbalance and salinization. In response, organic fertilizers have emerged as a crucial component for sustainable agricultural development. This study was designed to develop [...] Read more.
The use of chemical fertilizers has significantly increased crop yields but has also led to soil problems such as nutrient imbalance and salinization. In response, organic fertilizers have emerged as a crucial component for sustainable agricultural development. This study was designed to develop an easily applicable organic suspension fertilizer using peanut bran, the primary by-product of peanut oil extraction, as the main raw material. Fourier-transform infrared (FTIR) analysis revealed that 80 °C is the optimal heating temperature for forming a stable peanut-bran suspension. A comprehensive experimental investigation was conducted to evaluate the effects of different peanut bran addition levels, stabilizers, emulsifiers, and suspending agents on the stability of suspension fertilizers. The results identified the optimal suspension fertilizer formulation as comprising 20% peanut bran, 0.5% sodium bentonite, 0.1% monoglyceride, 0.2% sucrose ester, 0.02% carrageenan, and 0.3% xanthan gum. This formulation ensures good stability and fluidity of the suspension fertilizer while maintaining a low cost of 0.134 USD·kg−1. The findings provide a scalable technological framework for valorizing agro-industrial waste into high-performance organic fertilizers. Full article
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21 pages, 4939 KiB  
Article
Nitrogen-Fixing Bacterium GXGL-4A Promotes the Growth of Cucumber Plant Under Nitrogen Stress by Altering the Rhizosphere Microbial Structure
by Ying-Ying Han, Yu-Qing Bao, Er-Xing Wang, Ya-Ting Zhang, Bao-Lin Liu and Yun-Peng Chen
Microorganisms 2025, 13(8), 1824; https://doi.org/10.3390/microorganisms13081824 - 5 Aug 2025
Abstract
The rhizosphere microbiome plays an important role in carbon- and nitrogen-cycling in soil and in the stress response of plants. It also affects the function of the ammonium transporter (AmtB) that senses nitrogen levels inside and outside the cells of the associative nitrogen-fixing [...] Read more.
The rhizosphere microbiome plays an important role in carbon- and nitrogen-cycling in soil and in the stress response of plants. It also affects the function of the ammonium transporter (AmtB) that senses nitrogen levels inside and outside the cells of the associative nitrogen-fixing bacterium GXGL-4A. However, the potential mechanism of the interaction between the AmtB deletion mutant of GXGL-4A (∆amtB) and microorganisms in the rhizosphere of plants under low-nitrogen stress is still unclear. As revealed by transcriptome analyses, mutation of the amtB gene in GXGL-4A resulted in a significant up-regulation of many functional genes associated with nitrogen fixation and transportation at transcription level. The application of ∆amtB changed the nitrogen level in the rhizosphere of cucumber seedlings and reshaped the microbial community structure in the rhizosphere, enriching the relative abundance of Actinobacteriota and Gemmatimonadota. Based on bacterial functional prediction analyses, the metabolic capacities of rhizobacteria were improved after inoculation of cucumber seedlings with the original strain GXGL-4A or the ∆amtB mutant, resulting in the enhancement of amino acids, lipids, and carbohydrates in the cucumber rhizosphere, which promoted the growth of cucumber plants under a low-nitrogen stress condition. The results contribute to understanding the biological function of gene amtB, revealing the regulatory role of the strain GXGL-4A on cucumber rhizosphere nitrogen metabolism and laying a theoretical foundation for the development of efficient nitrogen-fixing bacterial agents for sustainable agricultural production. Full article
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180 KiB  
Proceeding Paper
Design of Automatic Generation Platform for Agricultural Robot
by Zhaowei Wang, Yurong Wang and Fangji Zhang
Eng. Proc. 2025, 98(1), 45; https://doi.org/10.3390/engproc2025098045 - 4 Aug 2025
Abstract
The design of robots is highly dependent on their applications. For agricultural robots, terrain, weather, and crop diversity need to be considered, and work efficiency, cost, and reliability must be evaluated. These factors are important to determine the design of agricultural robots. In [...] Read more.
The design of robots is highly dependent on their applications. For agricultural robots, terrain, weather, and crop diversity need to be considered, and work efficiency, cost, and reliability must be evaluated. These factors are important to determine the design of agricultural robots. In this study, we identified the constraint factors of agricultural robots from the perspectives of navigation, movement, control, cost, and reliability. The orthogonal defect classification (ODC) method was used to classify and grade these factors and explore the relationships among these factors. Based on the results, the design rules of agricultural robots were created, and an automatic production knowledge base of agricultural robot design was constructed. The results contribute to the automatic generation of the design framework of agricultural robots under specific environments to effectively improve the design level and quality of agricultural robots and popularize agricultural robots. Full article
34 pages, 9516 KiB  
Article
Proteus sp. Strain JHY1 Synergizes with Exogenous Dopamine to Enhance Rice Growth Performance Under Salt Stress
by Jing Ji, Baoying Ma, Runzhong Wang and Tiange Li
Microorganisms 2025, 13(8), 1820; https://doi.org/10.3390/microorganisms13081820 - 4 Aug 2025
Abstract
Soil salinization severely restricts crop growth and presents a major challenge to global agriculture. In this study, a plant-growth-promoting rhizobacterium (PGPR) was isolated and identified as Proteus sp. through 16S rDNA analysis and was subsequently named Proteus sp. JHY1. Under salt stress, exogenous [...] Read more.
Soil salinization severely restricts crop growth and presents a major challenge to global agriculture. In this study, a plant-growth-promoting rhizobacterium (PGPR) was isolated and identified as Proteus sp. through 16S rDNA analysis and was subsequently named Proteus sp. JHY1. Under salt stress, exogenous dopamine (DA) significantly enhanced the production of indole-3-acetic acid and ammonia by strain JHY1. Pot experiments revealed that both DA and JHY1 treatments effectively alleviated the adverse effects of 225 mM NaCl on rice, promoting biomass, plant height, and root length. More importantly, the combined application of DA-JHY1 showed a significant synergistic effect in mitigating salt stress. The treatment increased the chlorophyll content, net photosynthetic rate, osmotic regulators (proline, soluble sugars, and protein), and reduced lipid peroxidation. The treatment also increased soil nutrients (ammoniacal nitrogen and available phosphorus), enhanced soil enzyme activities (sucrase and alkaline phosphatase), stabilized the ion balance (K+/Na+), and modulated the soil rhizosphere microbial community by increasing beneficial bacteria, such as Actinobacteria and Firmicutes. This study provides the first evidence that the synergistic effect of DA and PGPR contributes to enhanced salt tolerance in rice, offering a novel strategy for alleviating the adverse effects of salt stress on plant growth. Full article
(This article belongs to the Section Plant Microbe Interactions)
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24 pages, 4519 KiB  
Article
Aerial Autonomy Under Adversity: Advances in Obstacle and Aircraft Detection Techniques for Unmanned Aerial Vehicles
by Cristian Randieri, Sai Venkata Ganesh, Rayappa David Amar Raj, Rama Muni Reddy Yanamala, Archana Pallakonda and Christian Napoli
Drones 2025, 9(8), 549; https://doi.org/10.3390/drones9080549 - 4 Aug 2025
Abstract
Unmanned Aerial Vehicles (UAVs) have rapidly grown into different essential applications, including surveillance, disaster response, agriculture, and urban monitoring. However, for UAVS to steer safely and autonomously, the ability to detect obstacles and nearby aircraft remains crucial, especially under hard environmental conditions. This [...] Read more.
Unmanned Aerial Vehicles (UAVs) have rapidly grown into different essential applications, including surveillance, disaster response, agriculture, and urban monitoring. However, for UAVS to steer safely and autonomously, the ability to detect obstacles and nearby aircraft remains crucial, especially under hard environmental conditions. This study comprehensively analyzes the recent landscape of obstacle and aircraft detection techniques tailored for UAVs acting in difficult scenarios such as fog, rain, smoke, low light, motion blur, and disorderly environments. It starts with a detailed discussion of key detection challenges and continues with an evaluation of different sensor types, from RGB and infrared cameras to LiDAR, radar, sonar, and event-based vision sensors. Both classical computer vision methods and deep learning-based detection techniques are examined in particular, highlighting their performance strengths and limitations under degraded sensing conditions. The paper additionally offers an overview of suitable UAV-specific datasets and the evaluation metrics generally used to evaluate detection systems. Finally, the paper examines open problems and coming research directions, emphasising the demand for lightweight, adaptive, and weather-resilient detection systems appropriate for real-time onboard processing. This study aims to guide students and engineers towards developing stronger and intelligent detection systems for next-generation UAV operations. Full article
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