Search Results (102)

Environmental DNA (eDNA) and environmental RNA (eRNA) are increasingly used in aquatic biomonitoring, but they serve different ecological purposes. eDNA can persist in biofilms, reflecting accumulated environmental signals, while eRNA is short-lived and indicates metabolically active communities. To assess their suitability for evaluating stream health, we compared benthic diatom assemblages derived from both eDNA and eRNA. Alpha diversity indices revealed negligible differences between the two nucleic acid types, although total read abundance showed a small effect size (Cliff’s δ = 0.207). Taxonomic overlap was significant, with 70% of species shared between the two sources, though several genera displayed source-specific patterns. Community analyses indicated that eRNA-based assemblages had greater internal consistency and clearer differentiation between groups. The Trophic Diatom Index application revealed that eDNA captured cumulative water quality conditions, while eRNA provided a more sensitive reflection of current ecological states. These findings highlight that eDNA offers broad taxonomic coverage, whereas eRNA delivers a higher resolution of active ecological responses. By integrating both markers, we can achieve a more comprehensive assessment of long-term environmental history and current stream health, enhancing the accuracy of diatom-based bioassessment frameworks. Full article

We compare the publication performance of open-access (OA) and subscription-based (SB) journals in Engineering using journal-level indicators from Scopus (CiteScore 2023 view; data collected on 2 December 2024). We analysed 3013 active Engineering journals with an assigned CiteScore quartile (Q1–Q4, where Q1 denotes the highest CiteScore quartile), of which 770 are labelled OA in Scopus; the remaining journals in each stratum were classified as SB. We stratified journals by CiteScore quartile and by the top 10% CiteScore percentile. We examined four indicators for 2020–2023: CiteScore 2023, total citations, number of published documents, and the percentage of cited articles. Because citation and publication counts are strongly right-skewed, we report medians and use Mann–Whitney tests with effect sizes (Cliff’s delta) and false discovery rate correction; Welch tests on log-transformed counts are used as sensitivity analyses. SB journals exhibit substantially higher citation and document medians across all quartiles and in the top 10% stratum, whereas CiteScore medians are very similar between access models. OA journals represent about one quarter of Engineering journals in Scopus, but remain underrepresented in the top 10% segment (125 of 484). Overall, OA provides a competitive level of impact, while SB titles still dominate accumulated visibility and editorial scale in Engineering. Full article

Background/Objectives: Systemic sclerosis-associated interstitial lung disease (SSc-ILD) and chronic obstructive pulmonary disease (COPD) are linked to multi-organ vulnerability involving the lungs, heart, and kidneys. This study aimed to compare the annual changes in pulmonary, cardiac, and renal parameters in patients with SSc-ILD and COPD across three consecutive years, using both individual biomarkers and integrated composite profiles. Methods: This observational longitudinal study included repeated assessments in 2023, 2024, and 2025. Functional, laboratory, and imaging parameters were collected: 6-min walk test (6MWT), SpO₂ (pre-/post-exercise), spirometry/CT lung volumes, gas exchange (pO₂/pCO₂/lactate), echocardiography [left ventricular ejection fraction (LVEF), estimated systolic pulmonary artery pressure (sPAP)], cardiac biomarkers (NT-proBNP, MR-proANP, hsTnT), renal markers [eGFR, creatinine, albuminuria, albumin-to-creatinine ratio (ACR)], heart rate variability (HRV), and renal CT densitometry. All markers were standardized (z-scores, higher values = worse). Subprofiles were generated and aggregated into three integrated profiles (cardiac, renal, pulmonary). Within-group dynamics were analyzed using the Wilcoxon signed-rank test (year-to-year deltas), between-group comparisons with the Mann–Whitney U test, effect sizes via Cliff’s delta, and multiple testing correction with the Benjamini–Hochberg false discovery rate (FDR). Results: Exercise tolerance declined in both groups: by 2025, 6MWT distance decreased by −10 m in SSc-ILD (p = 0.006; q = 0.010) and −20 m in COPD (p = 0.002; q = 0.004); post-exercise SpO₂ fell in both cohorts (both p < 0.001; q < 0.001). MR-proANP remained consistently higher in SSc-ILD across all years (p ≤ 0.005; q ≤ 0.028). sPAP increased in both groups, reaching higher values in COPD by 2025 (p = 0.007; q = 0.033). NT-proBNP and hsTnT increased over time, while eGFR declined, and ACR rose in both cohorts (both p < 0.001; q < 0.001). HRV (HF/total power) decreased by 2025. Composite profiles showed: in 2023, the cardiac profile was worse in SSc-ILD (δ ≈ 0.27; p = 0.011; q = 0.048), but differences diminished by 2025; the renal profile was initially worse in SSc-ILD but later shifted unfavorably in COPD; the pulmonary profile showed no consistent between-group differences. Conclusions: Over three years, patients with SSc-ILD and COPD exhibited concordant deterioration in pulmonary, cardiac, and renal function. Distinct leading markers emerged: desaturation during exercise and neurohormonal activation (MR-proANP) in SSc-ILD, versus reduced 6MWT and higher sPAP in COPD. These findings support the need for integrated monitoring of the cardio–pulmo–renal continuum. Limitations include the observational design, multiple comparisons, and absence of advanced repeated-measures modeling. Full article

Selenium-enriched Dendrobium huoshanense C. Z. Tang et S. J. Cheng is a precious medicinal herb that combines traditional therapeutic value with modern nutritional benefits. However, its wild populations primarily inhabit special habitats like cliffs and rock crevices, resulting in limited yield and low selenium content. This study optimized an in vitro selenium-enriched cultivation system for D. huoshanense, investigating the regulatory mechanisms of selenium on physiological metabolism by modulating exogenous selenium concentrations, and determining the spatiotemporal distribution and speciation of selenium in plantlets. The results showed the optimal medium composition was as follows: MS + IBA (0.1 mg/L) + NAA (0.6 mg/L) + 7% agar + 30% sucrose + 100 g/L banana homogenate + 3 mg/L sodium selenite (pH 5.8). Under these conditions, roots served as the primary selenium accumulation sites at 30 and 60 days of cultivation. After 90 days, selenium redistribution occurred from storage organs (roots) to metabolically active organs (leaves). Organic selenium constituted 83.70% of total selenium, comprising 44.90% selenoproteins, 29.20% selenopolysaccharides, and 9.60% other organic forms. The contents of selenomethionine (SeMet), methylselenocysteine (MeSeCys), and selenocysteine (SeCys₂) were 0.63 ± 0.04, 0.20 ± 0.11, and 0.28 ± 0.06 mg/kg, respectively. Using plant tissue culture technology, we successfully cultivated selenium-enriched D. huoshanense, and investigated its growth metabolism, selenium translocation mechanisms, and selenium speciation. These findings provide theoretical foundations for developing selenium-enriched medicinal materials and have significant implications for enhancing the medicinal value of D. huoshanense. Full article

Urban heat island conditions increase heat exposure and constrain safe outdoor activities. Urban forests can mitigate thermal loads; however, stand morphology can produce divergent microclimates. We aimed to quantify how stand type (open vs. closed), season (spring, summer, fall), and activity intensity (MET 1.0–6.0) jointly modulate thermal comfort and to identify season-specific optimal MET levels in an urban forest in Daejeon, Republic of Korea. We combined site-specific 3D canopy modeling with hourly Predicted Mean Vote (PMV) simulations driven by AMOS tower data (2023–2024). Comfort was defined as |PMV| ≤ 0.5. Analyses included seasonal means, Cliff’s delta, and generalized estimating equation logistic models to estimate the SITE × SEASON × MET interactions and predict comfort probabilities. Across most seasons and MET levels, C1 was more comfortable than C2. However, at MET 1.0 in summer, the pattern was reversed, which may reflect the canopy shading and associated decreases in mean radiant temperature (MRT) of C2. Comfort peaked at MET 2.0–3.0 and declined sharply at ≥4.5 MET. The three-way SITE × SEASON × MET interaction was significant (p < 0.001). The season-specific optimal MET values under our boundary conditions were 3.0 (spring), 2.0–2.5 (summer), and 3.0 (fall). These simulation-based PMV-centered findings represent model-informed tendencies. Nevertheless, they support actionable guidance: prioritize high-closure stands for low-intensity summer use, leverage open stands for low-to-moderate activities in spring and fall, and avoid high-intensity programs during warm periods. These results inform the programming and design of urban-forest healing and recreation by matching stand type and activity intensity to season to maximize comfortable hours. Full article

With the impact of fossil energy on the climate environment and the development of energy technologies, new energy vehicles, represented by electric cars, have begun to receive increasing attention and emphasis. The rapid proliferation of public charging infrastructure for NEVs has concurrently influenced traditional petrol station networks, creating measurable disparities in their spatial distributions that warrant systematic investigation. This research examines Wenzhou City, China, as a representative case area, employing multi-source Point of Interest (POI) data and spatial analysis models to analyse differential characteristics in spatial layout accessibility, service equity, and underlying driving mechanisms between public electric vehicle charging stations (EV) and traditional gas stations (GS). The findings reveal that public electric vehicle charging stations exhibit a pronounced “single-centre concentration with weak multi-centre linkage” spatial configuration, heavily reliant on dual-core drivers of population density and economic activity. This results in marked service accessibility declines in peripheral areas, resembling a cliff-like drop, and a relatively low spatial equity index. In contrast, traditional gas stations demonstrate a “core-axis linkage” diffusion pattern with strong coupling to urban road networks, showing gradient attenuation in service coverage efficiency along transportation arteries, fewer suburban service gaps, and more gradual accessibility reductions. Location entropy analysis further indicates that charging station deployment shows significant capital-oriented tendencies, with certain areas exhibiting paradoxical “excess facilities” phenomena, while gas station distribution aligns more closely with road network topology and transportation demand dynamics. Furthermore, the layout characteristics of public charging stations feature a more complex and diverse range of land use types, while traditional gas stations have a strong dependence on industrial land. This research elucidates the spatial distribution patterns of emerging and legacy energy infrastructure in the survey regions, providing critical empirical evidence for optimising energy infrastructure allocation and facilitating coordinated transportation system transitions. The findings also offer practical insights for the construction of energy supply facilities in urban development frameworks, holding substantial reference value for achieving sustainable urban spatial governance. Full article

Erosion represents a significant global threat to coastal zones, especially beaches, which are among the most valuable coastal landforms. This study evaluates the vulnerability to coastal erosion along the Brazilian Amazon coast, focusing on eight recreational beaches. The research is based on an assessment of geological, physical, ecological, and anthropogenic indicators. Some of these indicators were proposed in this study to enhance the evaluation of vulnerability in Amazonian beaches. The analysis reveals that most of the beaches studied are highly vulnerable to erosion due to a combination of natural factors and human activities. The barrier–beach ridge, composed of unconsolidated sediments, exhibits the highest vulnerability, while low cliffs present a moderate level of risk. The study highlights that semi-urban beaches with significant infrastructure development are particularly susceptible to erosion, a problem exacerbated by unplanned land use. Conversely, rural beaches, especially those located in protected areas, show lower vulnerability due to reduced human impact and better conservation of natural ecosystems. Furthermore, the study underscores the effects of extreme climatic events, such as prolonged rainfall and high-energy waves, which can intensify erosion risks. The findings suggest that anthropogenic changes, combined with extreme climate events, significantly influence the dynamics of coastal erosion. This research emphasizes the importance of targeted management strategies that address both natural and human-induced vulnerabilities, aiming to enhance coastal resilience and sustainability for Amazonian beaches. Full article

The demand for maritime–coastal tourism has been intensifying, but its offerings are sometimes limited to a few activities. Some of these activities do not require specific skills or certifications, while others do. This study aimed to investigate what type of activities are carried out by tourism and recreational vessels in the coastal area of the central Algarve (Portugal). To this end, data from the automatic identification system (AIS) of recreational vessels was used to monitor and categorise these activities in a non-intrusive manner. A model (TORMA) was defined to facilitate the analysis of AIS data and relate them to five independent variables (distance from the coast, boat speed, bathymetry, seabed type, and number of pings). The results of the analysis of more than 11 thousand hourly AIS records for passenger, sailing, and charter vessels showed that the 14 most regular ones had strong seasonal patterns, greater intensity in summer, and spatial patterns with more records near some coastal cliffs. This study provides valuable information on the management of motorised nautical activities near the coast and at sea, contributing to more informed and effective tourism regulation and planning. Full article

The geoheritage present on the “Tindari Cape and Marinello Lakes” site (TCML, Messina Province, NE Sicily, Italy) drew our attention due to the acquired contrasting information. Indeed, the TCML geoheritage was classified in the geosite national catalogue as a geosite (albeit under a non-evaluated status and with regional scientific interest), whereas it was classified in the geosite regional catalogue as a site of attention. The coastal geoheritage included in this site was analyzed by means of a literature review, field work, and a sedimentological and petrographic investigation. Moreover, the reconstruction of the historical to modern evolution of the lagoon and spit’s shapes was carried out across a time span of 85 years. The investigation results were used for the inventorying and quantitative assessment of the TCML geoheritage with the Brilha method. The primary and preeminent scientific interest was geomorphological, the lagoon and spit being an active geomorphosite. This system showed elements of rarity, representativeness, and exemplariness. Secondary-type geomorphological, structural, and palaeontological aspects were also evidenced in geological elements found on the cliffs of the Tindari Cape. For the quantitative assessment of the geodiversity, the scientific value (SV), potential educational use (PEU), potential touristic use (PTU), and degradation risk (DR) were evaluated. The obtained weighted scores were 320 (SV), 250 (PEU), 290 (PTU), and 285 (DR). The high SV suggested that the TCML, due to its geodiversity, could be classified as a geosite. Notwithstanding, the moderate PEU, PTU, and DR (fragility and vulnerability depending on natural climate and anthropogenic factors) values indicated that it was not fully compatible with educational and touristic purposes. The results of the inventorying and quantitative assessment of the TCML site provide scientific data that are useful in establishing the TCML as a global geosite, placing it in the national catalog of geosites. Full article

The Muong La–Bac Yen–Cho Bo fault is one of the seismic faults in the northwest region of Vietnam. Neo-tectonic activities and exogenous processes have influenced the drainage system and topographic–geomorphologic features on both walls of the fault. The results of topographic analysis and geomorphological indices have confirmed the active tectonics of the fault during the Neo-tectonic period (Pliocene–Quaternary, about 5 million years). The valley floor width-to-height ratio (V_f) of less than 0.5 indicates the “rejuvenation” of the streams and the obvious influence of tectonic activities on the two walls of the studied fault. The Hypsometric curve (HC) in the study area has a straight–convex shape and the basins on the northeast wall have hypsometric integral index values ranging from 0.46 to 0.481, which are lower and more convex than those of the southwest wall. The Mountain-front sinuosity index (S_mf) from 1.92 to 3.28 along the foot slope of the Hoang Lien Son range (the segment from Than Uyen to Bac Yen and Phu Yen) and the highly variable stream-length gradient index (SL) value on the northeast wall signify the relative tectonic uplift on the northeast wall of the fault. The deformed geomorphological indications (steep cliffs, slip surfaces, etc.) in the field confirm the active tectonics of the Muong La–Bac Yen–Cho Bo fault during the Neo-tectonic period. Full article

Background/Objectives: Hair loss affects self-esteem, confidence, and psychological well-being. Exosomes, as molecular carriers of growth factors and active compounds, offer a promising treatment. This study evaluates the efficacy of an exosome formulation containing extracts from two known hair-regenerating plants, Ecklonia cava and Thuja orientalis (ECPE), for male pattern alopecia. Methods: A randomized controlled trial included 20 male participants with Norwood grade 2–3 androgenetic alopecia who were randomly assigned into two groups, placebo (0.9% sodium chloride) and ECPE, administered bi-weekly across four sessions. Evaluations included hair density measurements, adverse effect tracking, and self-assessments. Results: Most participants (55%) were aged 18 to 35, with 75% reporting hair loss for over a year and 80% noting scalp thinning. The hair counts showed no significant change in the placebo group from baseline to week 16 (Wilcoxon signed-rank test: V = 13.5, p = 0.163), while a significant increase was observed in the ECPE group (V = 0, p = 0.002). Between-group analysis revealed a significant difference in the hair count changes (Wilcoxon rank-sum test: W = 86.5, p = 0.006) with a large effect size (Cliff’s Delta: & = 0.73, 95% CI: 0.41–0.89), with the ECPE group showing higher median hair growth (9.5, IQR = 16.88) compared to the placebo group (1.5, IQR = 3.00). A Bayesian ANCOVA, adjusted for covariates (the father’s scalp hair condition, baseline hair count, and Norwood classification), showed no significant effect of these factors on the outcomes. Conclusions: These findings suggest that ECPE significantly improves hair regrowth compared to the placebo, with no notable adverse effects. Full article

Maternal immune activation (MIA)—infection with an immunogen during pregnancy—is linked to an increased risk of neurodevelopmental disorders (NDDs) in offspring. Both MIA and NDDs are associated with developmental delays in offsprings’ motor behavior. Therefore, the current study examined the effects of MIA on neonatal reflex development in male and female offspring. Sprague Dawley rats were administered lipopolysaccharide (LPS; 50 μg/mL/kg, i.p.) or saline on embryonic day (E)15 of gestation. The offspring were then tested daily from postnatal day (P)3–P21 to determine their neonatal reflex abilities. The maternal care behaviors of the dam were also quantified on P1–P5, P10, and P15. We found that, regardless of sex, the E15 LPS offspring were able to forelimb grasp, cliff avoid, and right with a correct posture at an earlier postnatal age than the E15 saline offspring did. The E15 LPS offspring also showed better performance of forelimb grasping, hindlimb grasping, righting with correct posture, and walking with correct posture than the E15 saline offspring did. There were no significant differences in maternal licking/grooming, arched-back nursing, non-arched-back nursing, or total nursing across the E15 groups. Overall, these findings suggest that MIA with LPS on E15 accelerates reflex development in offspring without affecting maternal care. This may be explained by the stress acceleration hypothesis, whereby early-life stress accelerates development to promote survival. Full article

We conducted a helicopter survey in 2010 for cliff-nesting raptors along the Dalton Highway in northern Alaska. The study area extended from the Yukon River northward ~395 km through the Brooks Range to the Arctic Plain. We documented 55 occupied raptor nesting territories, including 25 Golden Eagle (Aquila chrysaetos), 11 Gyrfalcon (Falco rusticolus), and 10 Peregrine Falcon (Falco peregrinus) territories. We also recorded vacant stick nests and raptor perch sites on cliffs. We identified more occupied eagle territories and hundreds more vacant eagle stick nests than were previously known in the study area. The average number of Golden Eagle nestlings/nesting pairs at the time of the survey was 1.5 ± 0.6. The most productive Golden Eagle pairs were located in the northern part of the study area. The number of occupied eagle territories in 2010 is far fewer than the number of eagle stick nests we recorded. Our data indicate that the distribution of nesting eagles in the 2010 study area is different than in the past. Whether this reflects changes in eagle density; a response to availability of prey, climate change, human activities, or a combination of these; or some other factor warrants further investigation. Intensive surveys along the Dalton Highway in the 1970s found no occupied nests for Peregrine Falcons or Gyrfalcons but we found 10 and 11, respectively. Therefore, the Peregrine Falcon and Gyrfalcon nests we recorded in 2010 reflect an increase in the number of known nesting pairs there. Full article

Overcrowding in national parks and protected areas can cause irreversible damage to the environment, compromising the quality of soil, water, wildlife, and vegetation. Thus, it is critical for park managers to have detailed information on visitor activities and spatial dynamics in order to prioritise actions capable of mitigating undesirable impacts in the most frequently visited areas. In this article, we use georeferenced trails and photographs from the Wikiloc and Flickr web platforms to determine the spatial visitation patterns in the Atlantic Islands of Galicia National Park (AINP) from 2008 to 2023. Maps showing trail usage intensity and the distribution of photographs according to land use allowed us to identify the most frequented land uses by visitors and the areas of highest tourist pressure within the AINP. The results show that distribution patterns vary between platforms. Shrubland (37%) and marine cliffs (27%) were the most photographed land uses by visitors, while artificial areas (14%) were the most frequented by Wikiloc users. Cíes island emerges as the most popular tourist destination, as evidenced by the greater number of trails and photographs compared to Ons, Sálvora, and Cortegada. This study shows how social media data, specifically trails and geotagged photographs from Wikiloc and Flickr, can support and complement the monitoring of visitor use and impact in protected areas. Full article

The Lake Faro brackish basin (Sicily, Italy) was established as a Global Geosite as a key locality of tectonic coastal lakes, but little research has been devoted to this rare geological and ecological framework. To fill this gap, the main stratigraphical, sedimentological, ecological, morpho-bathymetric, and structural features were reported, linking geodiversity with biodiversity. In Lake Faro, a shallow platform develops alongside a deep funnel-shaped basin, reaching a maximum depth of 29 m. A NNW-SSE trending steep cliff, representing the abrupt transition from the platform to the basin, was interpreted as a dextral transtensional fault (Lake Faro Fault), presumably active since the middle-late(?) Pleistocene. The switches of the steep cliff NW-wards, acquiring an E-W trend, was interpreted as being due to the occurrence of the normal Mortelle Fault, cut by the Faro Lake Fault. Bottom terrigenous deposits consisted of coarse- to fine-grained quartzo-lithic rich sediments deriving from high-grade metamorphic and igneous rocks, whereas bioclasts mainly derived from clam farming actives for several centuries up until today. The Quaternary shallow platform, from top to base, includes the following: (i) soft cover composed of coarse terrigenous and prevalent bioclastic deposits; (ii) hard conglomerates cemented by carbonates; and (iii) siliciclastic coarse deposits of the Messina Fm. In the deep basin, siliciclastic silty loams with minor amounts of bioclastic deposits prevailed in the soft cover. Substrate heterogeneity coupled with brackish-marine gradients are the main factors responsible for an articulate patchiness of different lagoon habitats and related benthic associations, which, according to the Barcelona Convention classification, can be summarized as follows: (i) MB1.541 (marine angiosperms or other halophytes), (ii) MB1.542 (Fucales), (iii) MB5.543 (photophilic algae, except Fucales), (iv) MB5.544 (Facies with Polychaeta), and (v) MB5.545 (Facies with Bivalvia). Typical marine associations, such as rhodolite beds, also occur. Finally, the lake, which has been exploited since the prehistoric age because of its high biodiversity and productivity, maintains some evidence of millennial relationships with the resident human cultures, attracted there by the favorable geomorphological and ecological peculiarities. Full article