Search Results (1,297)

Although harbour seals have often bred successfully in captivity, there is little information on the behaviour and welfare of captive mothers and their pups. This study used video of a mother with her newborn pup (P1) during the first 16 days post-partum and further video of the same mother with a second pup (P2) during days 19–22. The most frequent behaviours in the water were body nosing and close body contact. The observations with pup P2 allowed for expanded descriptions of contact and following behaviours in the water. With P1, the mother displayed most responsibility for following and approaching, i.e., for maintaining contact. Suckling usually occurred immediately after the mother led the pup from the water to an onshore suckling location. P1 often rested alone while its mother checked on it continuously from the water and signalled it to return to the water by nudging, nosing and splashing at it. This mother engaged in close contact with both her pups, with both the mother and both pups displaying body language suggestive of positive emotion and, therefore, indicative of good welfare. These observations offer an argument in favour of allowing female harbour seals in captivity to give birth and wean their pups naturally. Full article

Itraconazole is an antifungal drug used to treat chytridiomycosis, one of the leading causes of global amphibian decline in species such as the critically endangered Panamanian golden frog (Atelopus zeteki). Despite its widespread use for prophylaxis and treatment in both assurance colonies and free-ranging amphibians, there is minimal pharmacologic information to guide dosing. In experiment A, frogs were exposed to 0.01% itraconazole for 10 min and tissue samples were analyzed at various time points from 1 to 84 h. In experiment B, frogs were divided into six groups and exposed to itraconazole in different combinations of concentration (0.01% or 0.001%) and time (5, 10, or 15 min) over 10 days of treatment. Tissue concentrations were quantified via high-performance liquid chromatography. In experiment A, following a one-time dose, itraconazole concentrations remained high until the end of the experiment at 84 h. In experiment B, at 0.01% itraconazole daily for 10 days, skin and liver concentrations were high and increased substantially over the 10-day treatment course. Frogs exposed at the lower concentration (0.001%) had tissue concentrations that appeared to remain steady. At the reported doses over 10 days, there was no histologic evidence of hepatic toxicity, although one frog was found dead in the low-dose bath at 84 h and could not be further analyzed. This experiment is an excellent example of assurance colonies providing evidence-based information for the improved care and welfare of amphibians in order to prepare for future free-ranging populations. Full article

Real-world time series forecasting is often challenging due to non-stationarity and distribution shifts, where the optimal forecasting model varies across different temporal regimes and horizons. In this work, we introduce a method called Adaptive Future-Guided Ensemble Learning (AFG-EL), a two-stage framework that performs drift-aware, sample-level routing over a heterogeneous model zoo. AFG-EL learns dynamic fusion weights from meta-features of the historical window and incorporates a future-guided training signal from a relative-future teacher or scorer, emphasizing learning on regime transitions and drift-sensitive segments. Crucially, the inference process remains strictly causal, requiring only historical data and extracted meta-features. We further use sparse routing with an entropy-based fallback mechanism to enhance stability when routing confidence is low. Our experiments on several commonly used forecasting datasets demonstrate that AFG-EL consistently outperforms strong single-model baselines, uniform averaging, and adaptive fusion baselines. Full article

Monitoring both captive animals and wild populations is necessary to ensure adequate animal welfare and wildlife conservation. Existing monitoring tools, e.g., camera traps, enable surveillance, yet analysis can prove time-consuming and labor-intensive if handled manually. The automated nature of machine learning (ML) reduces observer bias and manual workload and improves assessment capacity of behavioral monitoring tools that are often used by staff at zoological institutions. This study investigated the activity and space use of three captive jaguars (Panthera onca) through automated individual recognition, activity tracking, and heatmap visualization using an ML model trained on video footage. In total, 123.8 h of video footage was recorded of the jaguar enclosure in Randers Regnskov, Tropical Zoo. The ML model analyzed all videos containing jaguars from one day. The model achieved satisfactory performance based on its evaluation metrics (mean average precision, recall, precision, and F1-score). The ML model showed repeated movement tracks within specific enclosure areas. The jaguars exhibited significantly more inactive than active behavior and did not seem to exhibit natural bimodal nocturnal or crepuscular hunter activity patterns. It should be stated that, due to the small sample size of only three jaguars and 24 analyzed hours, this study is a proof-of-concept to demonstrate the potential of ML methods as valuable tools for individual recognition, activity tracking, and monitoring of space use to aid in future animal welfare monitoring. Full article

Hypoderma diana Brauer, 1858, poses a significant threat to species of deer, yet records of this species in China remain fragmented and lack comprehensive documentation. Following high mortality in wapiti populations in Inner Mongolia during 2023–2024, surveys were conducted in the Gaogesitai Hanwula Nature Reserve in March 2025. Species identity was established through rigorous morphological analysis of third-instar larvae and an emerged adult female using light and scanning electron microscopy, supplemented by a 689 bp COI fragment. Results confirmed H. diana as the causative agent, with larvae localized along the host’s vertebral column. Comparative SEM analysis of the antennomaxillary sensory complex in H. diana, H. bovis (Linnaeus, 1758), and H. lineatum (Villers, 1789) revealed undifferentiated, volcano-like warts, contrasting sharply with the highly differentiated antennae and sensilla of Oestrus ovis Linnaeus, 1758. This study represents an inaugural record of H. diana in the Chifeng region and provides the first atlas of diagnostic features for both third-instar larvae and adults. These findings document H. diana as a critical agent of myiasis in the Manchurian wapiti, refining its known epidemiological profile in China and establishing an essential morphological reference for future wildlife health surveillance. Full article

Grotta-Riparo di Uluzzo C (Uluzzo Bay, Apulia, southern Italy) preserves a long and complex stratigraphic sequence spanning from the Middle Palaeolithic to the Bronze Age, offering a valuable context for investigating depositional dynamics and human–environment interactions during the Late Pleistocene. Although recent multidisciplinary research has substantially advanced knowledge of the Uluzzian occupations, the Mousterian faunal record of the site has remained largely unexplored from zooarchaeological and taphonomic perspectives. This study examines the faunal assemblages from the Mousterian layers (E, F, and G), integrating material from historical excavations with those recovered during recent fieldwork. Zooarchaeological, taphonomic, and Zooarchaeology by Mass Spectrometry (ZooMS) analyses are combined to reconstruct local environmental conditions, evaluate the relative contribution of human and non-human agents to bone accumulation, and assess patterns of site use and deposit formation. The faunal spectrum indicates an ecologically heterogeneous landscape, consistent with a Mediterranean refugial setting during the Late Pleistocene. Taphonomic evidence points to complex and cumulative formation processes resulting from repeated, short-term human occupations interspersed with carnivore activity and natural depositional processes. The Mousterian deposits are therefore best interpreted as brief palimpsests rather than the result of continuous or intensive occupation. Placed within a regional framework, the Uluzzo C assemblages contribute to broader discussions on site formation processes and environmental variability in southern Italy and provide an important comparative baseline for the Middle to Upper Palaeolithic period. Full article

Although canine parvovirus (CPV) vaccination has been widely implemented, CPV continues to circulate in dog populations and poses a potential cross-species transmission risk to wildlife, including giant pandas. Recent increases in CPV-2c detection in China highlight the need for molecular surveillance and standardized immunoreagents for diagnosis and epitope mapping. This study aimed to isolate a representative CPV-2c strain from China and develop VP2-targeted nanobody-based recognition molecules to support antigen monitoring and detection optimization. Canine and giant panda samples were collected in Sichuan Province, and CPV was isolated in F81 cells, followed by VP2 gene sequencing and phylogenetic analysis. A secretion expression system in Bacillus subtilis was established to produce VP2-targeting nanobodies, and a canine Fc-fused format of Nb10 (Nb10-Fc) was constructed. Immunoreactivity was evaluated via immunoassays, and structural modeling and molecular docking were performed to predict binding interfaces. The results showed that CPV-2c was the dominant genotype in Sichuan, with CPV L4 being a representative strain that exhibited 100% identity in VP2 with a giant panda-derived CPV-2c strain. Nb10 and Nb10-Fc demonstrated strong reactivity in Western blotting and immunofluorescence assays. The Fc-fusion improved detection sensitivity, offering potential in vivo application benefits. This study provides a standardized VP2-specific nanobody and molecular system for CPV-2c surveillance, antigenic studies, and diagnostic optimization. Full article

Galaxy morphology combines measurable structural properties with subjective visual interpretation, limiting strictly hard-label classifications. This study proposes a framework designed to compare physically derived and human-based galaxy classifications while explicitly accounting for uncertainty and interpretability. Using photometric and structural features from the Sloan Digital Sky Survey (SDSS), physical groupings are obtained through Fuzzy C-Means clustering, enabling gradual transitions via soft memberships. Human clusters are constructed from Galaxy Zoo 2 debiased vote fractions, capturing aggregated perceptual judgments. Supervised models are trained to predict both physical and human cluster assignments from the same set of physical variables, providing a quantitative assessment of structural coherence and perceptual–physical alignment. SHAP-based explainability identifies the relative influence of color and concentration parameters in each scheme. Results show that physical clustering is driven by structural concentration and bulge dominance, while human classification exhibits smoother decision boundaries and greater sensitivity to photometric appearance. Discrepancies concentrate in transitional and orientation-sensitive systems. An interactive visualization layer supports traceable qualitative inspection. The framework provides a reproducible methodology for analyzing classification consistency, uncertainty, and human–model alignment. Full article

Effective wolf monitoring is more critical than ever for supporting robust population estimates, identifying breeding packs, and anticipating and mitigating attacks on livestock. This study evaluates bioacoustic monitoring to estimate wolf population size and detect packs and pups based on howling activity, using recordings collected from free-ranging wolves in four different study areas in Denmark. It assesses whether howl structure can be used to identify wild individuals and discriminate between current-year pups (aged between four and nine months) and adults. At wild Location 1, we identified two free-ranging individuals from 40 adult howls by quantifying fundamental frequency features and applying linear discriminant and multivariate variance analyses; the individual classification accuracy was 92%. We examined howls recorded from late August 2021 to February 2022 using maximum fundamental frequency. Across months, mixture modelling and principal component analysis consistently resolved two groups in the wild data, and multivariate tests indicated significant separation each month (p < 0.001), consistent with a pup–adult contrast and the expected autumnal decline in pup frequencies as they mature. A focused analysis restricted to adult-range howls also resolved two groups with very strong multivariate separation (p < 0.001), but this pattern is considered exploratory and does not constitute confirmed sex discrimination. Overall, passive bioacoustics is an effective, non-invasive approach for wide-area monitoring, individual-level acoustic identification, and detecting the presence of pups within wolf packs; however, inference about pack composition or sex based solely on acoustic clustering requires further validation. Full article

Zoological parks increasingly operate as sustainability-oriented institutions that integrate biodiversity conservation, environmental education, animal welfare, and community engagement. In parallel with these evolving roles, digital and interactive technologies have emerged as key instruments for supporting sustainable visitor engagement and conservation communication. This study provides a systematic review and conceptual mapping of the literature by combining a PRISMA-based systematic literature review with bibliometric co-word analysis. The bibliometric results reveal four thematic clusters: (1) mobile and visitor-oriented digital technologies, (2) immersive augmented reality (AR) or virtual reality (VR) based solutions, (3) animal–computer interaction and welfare-focused technologies, and (4) traditional conservation and education research. While digital technologies demonstrate measurable short-term effects on engagement, empathy, and knowledge retention, their long-term sustainability impact and systemic integration remain limited. To address this gap, the study introduces three theoretical contributions: the concept of the zoo-based digital learning ecology, the identification of the digital fragmentation problem, and the Integrated Digital Zoo Ecosystem (IDZE) model. The proposed framework conceptualizes digital visitor experience, animal welfare technologies, and conservation communication as interdependent subsystems within a unified sustainability-oriented ecosystem. This study provides a conceptual foundation for future sustainability-driven digital innovation in zoological parks. Full article

Misiones, Argentina, holds one of the largest remnants of the Atlantic Forest, with almost 1.4 million hectares of native forest, representing a critical landscape for sustainable biodiversity conservation. However, connectivity across this ecoregion is increasingly threatened by habitat conversion, landscape fragmentation, and poaching pressures that extend beyond protected area boundaries, undermining long-term sustainability of wildlife populations. Using conservation detection dogs, we located, collected, and genetically confirmed 198 scats belonging to four game species: 20 lowland tapir (Tapirus terrestris), 72 white-lipped peccary (Tayassu pecari), 55 collared peccary (Pecari tajacu), and 51 Azara’s agouti (Dasyprocta azarae). Analyses examining species-specific habitat associations emphasized the importance of extending inference beyond point locations to encompass species’ home ranges, with native forest consistently identified as a key component of habitat use. The high prevalence of scats in mosaics of human-modified habitats outside protected areas, especially along their borders, underscores the importance of managing these areas as part of a broader sustainable landscape matrix. While native forest fragments outside of protected areas may serve as important refugia supporting species persistence, their contribution to sustainable management depends on reducing poaching pressure across these landscapes. There is an urgent need to expand antipoaching efforts beyond protected areas and across the Atlantic Forest in the Green Corridor of Misiones while preventing ongoing deforestation and the expansion of monoculture plantations. Achieving sustainable wildlife management in this region will require integrated strategies that promote sustainable land use, conservation planning, and rural development. Full article

The aim of this study is to conduct a comprehensive assessment of the water quality of the Sidi Mohammed Ben Taiba (SMBT), one of the largest drinking water reservoirs in northwestern Algeria, by integrating chemical and biological indicators. The assessment combines the Drinking Water Quality Index (DWQI), the Irrigation Water Quality Index (IWQI), the Organic Pollution Index (OPI) and zooplankton-based biological indicators (Zoo-IQ). A total of 23 physicochemical parameters were analyzed and interpreted using multivariate statistical approaches. This study fills an important knowledge gap by evaluating long-term temporal variability (January 2018–May 2025) and recent spatial heterogeneity (June 2023–May 2025), aiming to support sustainable water management. The results indicate that the reservoir water quality is generally suitable for drinking purposes (22.3 < DWQI < 54.0), is deemed excellent for agricultural irrigation (65 < IWQI < 69) and that the reservoir surface waters are slightly polluted to unpolluted (0.3 < OPI < 1.1). However, a deterioration in water quality has been detected in recent years, linked to increasing nutrient concentrations, as confirmed by the TSI–SD index. Despite the early signs of nutrient enrichment, the Zoo-IQ index remained within the moderate to good range, suggesting a certain degree of resilience in the zooplankton community. However, pronounced seasonal fluctuations observed in the Zoo-IQ and species diversity (H′) during periods of environmental stress serve as an early warning signal of emerging problems that may negatively affect water quality indices (WQI, IWQI, OPI). Station S4, located at the confluence of Wadi Belhassen and Wadi Farhat, descending from the Dahra mountain range in Algeria, has been identified as the most sensitive area and a potential hotspot for future pollution. The study provides robust data on the quality of reservoir water, offering a valuable decision-making tool for artificial reservoir managers and contributing to sustainable water management by identifying risk areas and supporting the implementation of preventive measures. Full article

This narrative review examines the clinical application of tiletamine–zolazepam (TZ) in exotic pet and wildlife anesthesia, addressing the complexities inherent in managing a broad taxonomic spectrum with diverse physiological profiles and temperaments. As a fixed-dose combination, TZ is a cornerstone of multimodal protocols designed to achieve balanced anesthesia. Its lyophilized formulation permits reconstitution with various sedative solutions, facilitating low-volume administration, a critical requirement for the immobilization of wildlife and small exotic patients. Given the significant variability in species-specific responses and environmental influences, selecting and adapting appropriate TZ-based protocols remain a challenge for practitioners. By synthesizing heterogeneous data into expert-validated guidance, this review provides specialized and general veterinarians with practical considerations for the judicious use of TZ. Emphasis is placed on integrating TZ within multimodal protocols to mitigate arousal risks, ensure consistent immobilization, and facilitate rapid recovery. This approach seeks to optimize anesthetic outcomes and promote animal welfare across these physiologically diverse populations. Full article

Air quality management in zoological gardens plays a crucial role in their sustainable development. However, air quality in these settings remains understudied. In addition, previous research has largely focused on airborne microbial contamination merely in animal enclosures. This exploratory study provides preliminary insights into airborne fungal contamination alongside microclimate conditions in the visitor and worker areas of animal premises in the Zagreb Zoo. The study was performed in the Monkey House, Tropical House, Rainy Africa, and Bird House, as well as outdoors in fall. Fungi were identified based on macroscopic and microscopic examinations. Total culturable fungal concentration in indoor air ranged between 50 and 4.25 × 10³ CFU/m³, and in outdoor air between 1.00 × 10² and 1.50 × 10³ CFU/m³. Molds of eight genera and yeasts were isolated from the air. Both indoors and outdoors, the predominant genera were Cladosporium and Penicillium, and also genus Aspergillus indoors. Cladosporium spp. and Penicillium spp. concentrations, as well as total fungal concentration in the air, were on average, highest in Rainy Africa and Bird House, while the highest average Aspergillus spp. concentration was found in the Tropical House. Levels of Cladosporium spp., Penicillium spp., and Aspergillus spp. concentrations were associated with microclimate conditions. Study results suggest that the airborne fungal contamination may depend on the animals housed in the premises, and the design and management of the premises. Although total fungal concentration determined may not necessarily pose a health risk for exposed people, the qualitative composition of fungi signifies the importance of implementing good practices in zoo premises, including optimal microclimate conditions and effective ventilation. The results obtained also indicate the need for air quality monitoring, which concurs with zoo sustainability goals. Full article