Search Results (604)

The present study analyzes individual differences in the facilitative processing of grammatical gender by heritage speakers of Spanish, asking whether these differences correlate with lexical proficiency. Results from an eye-tracking study in the Visual World Paradigm replicate prior findings that, as a group, heritage speakers of Spanish show facilitative processing of gender. Importantly, in a follow-up within-group analysis, we test whether three measures of lexical proficiency—oral picture-naming, verbal fluency, and LexTALE—predict individual performance. We find that lexical proficiency, as measured by LexTALE, predicts overall word recognition; however, we observe no effects of the other measures and no evidence that lexical proficiency modulates the strength of the facilitative effect. Our results highlight the importance of carefully selecting tools for proficiency assessment in experimental studies involving heritage speakers, underscoring that the absence of evidence for an effect of proficiency based on a single measure should not be taken as evidence of absence. Full article

Effective species identification is crucial for the conservation and management of marine mammals, particularly in regions such as the Mediterranean Sea, where several cetacean populations are endangered or vulnerable. In this study, we developed and validated a High-Resolution Melting (HRM) analysis protocol for the rapid, cost-effective, and reliable identification of the four representative marine cetacean species that occur in the Mediterranean Sea: the bottlenose dolphin (Tursiops truncatus), the striped dolphin (Stenella coeruleoalba), the sperm whale (Physeter macrocephalus), and the fin whale (Balaenoptera physalus). Species-specific primers targeting mitochondrial DNA regions (cytochrome b and D-loop) were designed to generate distinct melting profiles. The protocol was tested on both tissue and fecal samples, demonstrating high sensitivity, reproducibility, and discrimination power. The results confirmed the robustness of the method, with melting curve profiles clearly distinguishing the target species and achieving a success rate > 95% in identifying unknown samples. The use of HRM offers several advantages over traditional sequencing methods, including reduced cost, speed, portability, and suitability for degraded samples, such as those from the stranded individuals. This approach provides a valuable tool for non-invasive genetic surveys and real-time species monitoring, contributing to more effective conservation strategies for cetaceans and enforcement of regulations against illegal trade. Full article

tRNA modifications are crucial for efficient protein synthesis, impacting codon recognition, tRNA stability, and translation rates. RNA viruses hijack the host’s translational machinery, including the pool of modified tRNA, to translate their own genomes. However, the mismatch between viral and host codon usage can lead to a limited availability of specific tRNA leading to ribosome stalling, posing a significant challenge for efficient protein translation. While some viruses address this challenge through codon optimization, we show here that SARS-CoV-2 (Coronavirus) and the Zika virus (ZIKV; Flavivirus) adopt a different approach, manipulating the host tRNA epitranscriptome. Analysis of codon bias indices confirmed a substantial divergence between viral and host codon usage, revealing a strong preference in viral genes for codons decoded by tRNAs requiring U34 wobble modification. Monitoring tRNA modification dynamics in infected cells showed that both SARS-CoV2 and ZIKV enhance U34 tRNA modifications during infection. Strikingly, impairing U34 tRNAs profoundly impacted viral replication, underscoring the strict reliance of SARS-CoV-2 and ZIKV on manipulating the host tRNA epitranscriptome to support the efficient translation of their genome. Full article

Background/Objectives： Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a prevalent Mendelian disorder caused by mutations in the NOTCH3 gene, primarily impacting cerebral small blood vessels. This review aims to explore the involvement of large intracranial arteries in CADASIL, particularly focusing on the association with RNF213 polymorphisms, especially in Asian populations. Methods： A comprehensive literature review was conducted to gather data on the morphological features of both small and large intracranial arteries in CADASIL, examining clinical manifestations, imaging findings, and genetic associations. Results： The findings indicate that while CADASIL is predominantly characterized by small vessel disease, a significant number of patients also exhibit large artery involvement, particularly Asian populations where RNF213 polymorphisms may play a critical role. The review highlights the evidence of intracranial stenosis and the potential implications of traditional vascular risk factors, such as hypertension and diabetes mellitus, which are prevalent in these populations. Conclusions： The involvement of larger intracranial arteries in CADASIL underscores the complexity of the disease, suggesting that both genetic predispositions and environmental factors contribute to vascular abnormalities. Further research is needed to clarify these relationships and improve diagnostic and therapeutic strategies for CADASIL patients. Full article

Two of the renowned figures of Edo-era Kokugaku (National Learning), Motoori Norinaga and Ueda Akinari, famously debated the merits of their scholarly approaches to Japanese antiquity during the latter half of the eighteenth century. Their intellectual dispute was the result of the radical conclusions reached by Tō Teikan in his Shōkōhatsu (An Outburst of Provocations; 1781) in which he argued that the Korean peninsula and China influenced ancient Japan, and that Japan’s first emperor, Jimmu, was from Ryukyu. While Akinari supported the notion of continental influence on ancient Japan, Norinaga did not, and while the former was mostly agnostic about Jimmu’s Ryukyuan roots, the latter opposed that as well. Norinaga, however, was not opposed to the idea of ancient ties between Ryukyu and Japan, an issue with which Akinari’s silence seemed to signify some degree of agreement. This commonality between these two intellectual giants demonstrated the extent to which Japanese intellectuals of the Edo period viewed the Ryukyu Kingdom (now Okinawa Prefecture) as occupying an ambivalent geopolitical space, in which it was neither fully foreign nor fully native. At the same time, Akinari’s historiographical approach to Japanese antiquity, which emerged in his debate with Norinaga, exerted an influence on nineteenth-century depictions of Ryukyu’s historical and cultural ties to Japan, chiefly Kyokutei Bakin’s Chinsetsu yumiharizuki (Fantastic Tales of the Moon Bow; 1811). Full article

Dissolved oxygen (DO) is widely recognized as a fundamental parameter in assessing water quality, given its critical role in supporting aquatic ecosystems. Accurate estimation of DO levels is crucial for effective management of riverine environments, especially in anthropogenically stressed regions. In this study, a hybrid machine learning (ML) framework is introduced to predict DO concentrations, where optimization is performed through Genetic Algorithm Search with Cross-Validation (GASearchCV). The methodology was applied to a dataset collected from the Sitnica River in Kosovo, comprising more than 18,000 observations of temperature, conductivity, pH, and dissolved oxygen. The ML models Elastic Net (EN), Support Vector Regression (SVR), and Light Gradient Boosting Machine (LGBM) were fine-tuned using cross-validation and assessed using five performance metrics: coefficient of determination (${\mathrm{R}}^2$), root mean square error (RMSE), mean absolute error (MAE), mean absolute relative error MARE, and mean square error (MSE). Among them, the LGBM model yielded the best predictive results, achieving an ${\mathrm{R}}^2$ of 0.944 and RMSE of 8.430 mg/L on average. A Monte Carlo Simulation-based uncertainty analysis further confirmed the model’s robustness, enabling comparison of the trade-off between uncertainty and predictive precision. Comparison with recent studies confirms the proposed framework’s competitive performance, demonstrating the effectiveness of automated tuning and ensemble learning in achieving reliable and real-time water quality forecasting. The methodology offers a scalable and reliable solution for advancing data-driven water quality forecasting, with direct applicability to real-time environmental monitoring and sustainable resource management. Full article

Dietary choices and patterns have enormous consequences along the lines of individual, community, and planetary health. Excess meat consumption has been linked to chronic disease risk, and at large scales, the underlying industries maintain a massive environmental footprint. For these reasons, public and planetary health experts are unified in emphasizing a whole or minimally processed plant-based diet. In response, the purveyors of ultra-processed foods have added “meat alternatives” to their ultra-processed commercial portfolios; multinational corporations have been joined by “start-ups” with new ultra-processed meat analogues. Here, in our Viewpoint, we revisit the 1990s food industry rhetoric and product innovation, a time in which multinational corporations pushed a great “low-fat transition.” We focus on the McLean Deluxe burger, a carrageenan-rich product introduced by the McDonald’s Corporation in 1991. Propelled by a marketing and media-driven fear of dietary fats, the lower-fat burger was presented with great fanfare. We reflect this history off the current “great protein transition,” a period once again rich in rhetoric, with similar displays of industry detachment from concerns about the health consequences of innovation. We scrutinize the safety of carrageenan and argue that the McLean burger should serve as a cautionary tale for planetary health and 21st century food innovation. Full article

Cardiac magnetic resonance (CMR) imaging provides significant advantages in the non-invasive diagnosis of cardiac diseases. An emerging phenotype is increasingly being described in CMR reports, the LGE “ring-like” pattern, which resembles a circumferential/semi-circumferential LV scar. Different conditions exhibit this fibrosis distribution, the majority of them being genetically determined and mostly involving cardiomyopathy-causative genes (desmosomal but also other non-desmosomal related genes). Furthermore, inflammatory diseases, such as myocarditis or sarcoidosis, could be responsible for LV fibrosis, potentially exhibiting an RL distribution. Given the heterogeneity of such conditions, effective patient management requires a stepwise and multiparametric diagnostic work-up that integrates clinical, instrumental, and genetic data to identify the specific aetiology and guide personalised treatments. Full article

We report two cases of prolonged “unexpected” asystoles in patients with a wearable cardioverter-defibrillator (WCD) and a subcutaneous implantable cardioverter-defibrillator (ICD), respectively, which were promptly recognized and successfully managed. As these devices are designed to recognize and treat malignant tachyarrhythmias but do not provide pacing capabilities, it is crucial to identify patients with paroxysmal conduction disorders who might require backup pacing. For this reason, it is also important to leverage the monitoring features of both devices and their ability to detect the occurrence of bradyarrhythmias. Full article

In the realm of click-type reactions and their application to bioorthogonal chemistry in living organisms, metal-free [3+2] cycloadditions involving mesoionic rings and strained cycloalkynes have gained increasing attention and potentiality in recent years. While there has been a significant accretion of experimental data, biological assays, and assessments of reaction mechanisms, some pieces of the tale are still missing. For instance, which structural and/or stereoelectronic effects are actually interlocked and which remain unplugged. With the advent of data-driven methods, including machine learning simulations, quantitative estimations of relevant observables and their correlations will explore better the chemical space of these transformations. Here we unveil a series of linear relationships, such as Hammett-type correlations, as well as deviations of linearity, using the case study of phenylsydnone (and its 4-aryl-substituted derivatives) with a highly reactive bicyclo[6.1.0]nonyne carbinol. Through accurate estimation of activation barriers and prediction of rate constants, our findings further increase the significance of integrating strain release and electronic effects in organic reactivity. Moreover, such results could pave the way to use mesoionics cycloadditions as probes for measuring the extent of delocalization-assisted strain release, which can be applied to related reactions involving dipoles and strained rings. Full article

This study aims to photograph, design, and digitally document the surviving residential buildings on the island of Halki (Heybeliada), within the Princes’ Islands. This documentation focuses on the architectural, urban, and historical aspects of Halki, highlighting the significant material evidence of the Greek social and economic presence. It also examines the urban cultural heritage as depicted in Turkish literature of that period to understand how Turkish writers perceived and presented Halki, referencing the Princes’ Islands only for background context. The methodology includes the collection of material from residents through bibliographic and field research conducted on Halki. Based on these findings, a mobile augmented reality (AR) application was developed using the TaleBlazer platform, designed specifically for use on Halki. The application provides a virtual tour with multimedia-supported thematic layers of architectural and historical information. Its usability and learnability were evaluated using a questionnaire completed by students. The results showed high usability, user satisfaction, and perceived value of learning, with the majority of results close to a median score of 4 out of 5. The students identified the occurrence of immersive experience, ease of use, and the emotional stimulation created by the integration of spatial storytelling and multimedia. This paper also shows how the convergence of cultural content (history, architecture, and literature) can enhance interpretations and experiences with mobile AR technologies. Full article

On 11 March 2020, the World Health Organisation (WHO) declared a global pandemic caused by the SARS-CoV-2 coronavirus that earlier in February 2020 the WHO had named COVID-19 (coronavirus disease 2019). There were neither drugs nor vaccines that were known to be effective against the virus, stimulating an urgent worldwide search for treatments. An evaluation of existing drugs by ‘repurposing’ was initiated followed by a transition to de novo drug discovery. Repurposing of an already approved drug may accelerate the introduction of that drug into clinical use by circumventing early, including preclinical studies otherwise essential for a de novo drug and reducing development costs. Early in the pandemic three drugs were identified as repurposing candidates for the treatment of COVID-19: (i) hydroxychloroquine, an anti-malarial also used to treat rheumatoid arthritis and lupus; (ii) ivermectin, an antiparasitic approved for both human and veterinary use; (iii) remdesivir, an anti-viral originally developed to treat hepatitis C. The scientific evidence, both for and against the efficacy of these three drugs as treatments for COVID-19, vied with public demand and politicization as unqualified opinions clashed with evidence-based medicine. To quote Hippocrates, “There are in fact two things, science and opinion; the former begets knowledge, the latter ignorance”. Full article

The transcription factor known as TALE (three-amino acid loop extension) is essential for plant growth, cell differentiation and responses to environmental stresses. Although the TALE gene family has been identified in various plants, there has been a lack of comprehensive whole-genome identification and analysis in Gramineae species. In this study, 123 TALE family genes were identified in five Gramineae species, which can be categorized into two main subgroups: KONX and BELL. Most of the TALE genes in the same subgroup displayed analogous gene structures and conserved motifs. Furthermore, whole genome duplication (WGD) significantly contributes to the expansion of the TALE gene family in Gramineae. The promoter region of TALE genes in Gramineae contains a large number of cis-elements associated with abiotic stress and hormone response. Tissue-specific expression analysis indicated that most OsTALE, ZmTALE and AtTALE genes were highly expressed in stems and leaves. Additionally, RNA-seq data revealed that OsTALE, ZmTALE and AtTALE genes were found to respond to abiotic stress treatments. Furthermore, we found that the expression levels of SbTALE11/19 were up-regulated in response to PEG and NaCl treatment, respectively. This study provides a significant reference for further research on the biological function of TALE transcription factors in Gramineae plants. Full article

This essay explores themes in monk–underworld stories from the early medieval to Song periods, shedding light on evolving monastic–lay relations. These tales typically follow an individual who visits the afterlife and returns to share their experience. Monks frequently appear in these narratives, revealing shifting perceptions of the clergy. In earlier stories, monks often serve as underworld guardians or bodhisattvas, but by the Song period they are more commonly depicted as recipients of punishment, often for neglecting their obligations to lay patrons. This shift suggests that Song society increasingly viewed monks through a commercial lens, expecting them to provide specific services. However, these underworld tales do not indicate a decline in respect for the clergy. A broader look at Song literature confirms that monks remained highly regarded, despite changing expectations of their role within society. Full article

Creole writer Sidonie de la Houssaye (1820–1894) registered the threat of anglophone dominance after the Civil War on behalf of a host of characters drawn from the geographies and ideologies in and around her home in Louisiana. Her little-known literary tales depict the period as a cultural and linguistic border zone. In addition to the texture of Louisiana French and Creole heritage, the tales depict the vexed social dynamics of prejudice and fragility. In the context of this special issue on good and evil, the poorly known children’s tales offer insight into these pernicious tensions that persisted under the surface of moral victory after the Civil War. La Houssaye’s lessons for children take up the moral panic of a Louisiana reckoning with its legacies of racial violence and cultural erasure. This article argues that morality in these tales takes shape in interpersonal practices that can be learned to heal social ills. What I have called La Houssaye’s “ethics of social life” relies on education rather than condemnation to redefine human bonds. If a broader lesson emerges from the stories taken together, it suggests that structural change is slow to heal cultural wounds. We must ourselves be the agents of a healthier community. Full article