Search Results (626)

Chronic and diabetic wounds are characterized by persistent inflammation, excessive oxidative stress, and impaired cellular responses, collectively leading to delayed tissue repair. This study aimed to evaluate a thermoresponsive gel incorporating Aloe vera (AV) extracts and silk fibroin (SF), focusing on its biocompatibility, anti-inflammatory, antioxidant, and pro-regenerative potential. The AV: SF mixture and gel formulation were evaluated in macrophage-like THP-1 cells, and human diabetic dermal fibroblasts (HDdF) under non-induced and LPS/IL-6/TNF-α-induced inflammatory conditions. Both formulations maintained high cell viability across all cell types, indicating good biocompatibility. Under inflammatory conditions, both the AV: SF mixture and gel significantly reduced intracellular ROS levels and suppressed TNF-α and IL-1β secretion. In addition, NF-κB p65 activation was attenuated in both macrophage-like THP-1 cells and HDdF. Macrophage-like THP-1 cells displayed the M2 (CD80^low/CD206^high) phenotype, indicating modulation of macrophage polarization toward wound remodeling and repair. Overall, the AV: SF-based gel system demonstrated coordinated antioxidant, anti-inflammatory, and pro-regenerative effects in vitro. These findings support its potential as a multifunctional biomaterial for modulating inflammation-associated cellular dysfunction in chronic and diabetic wound environments. Full article

Rubber aggregate concrete (RAC) offers excellent frost resistance and impact toughness, but the incorporation of rubber particles substantially reduces its compressive strength, limiting structural applications. Existing improvement strategies have mainly relied on a single modification route, whereas the dynamic compressive response of RAC containing both ceramic balls and steel fibers remains insufficiently clarified. Here, a ceramic ball–steel fiber rubber aggregate concrete (CBSFRC) was investigated using quasi-static compression, a Φ120 mm split Hopkinson pressure bar system, and high-speed photography. Three steel-fiber volume fractions (1.0%, 1.5%, and 2.0%) were tested. The CBSFRC waveform displayed a characteristic ‘low-first, high-second’ double-peak pattern, in contrast to the ‘high-first, low-second’ pattern of the reference rubber aggregate concrete (CRC). At the common interpolated strain rates of 40, 45, and 50 s⁻¹, the dynamic compressive strengths of CBSFRC were 35.9–53.6% higher than those of CRC; no extrapolation was used. The largest quasi-static strength increase among the tested mixtures was 51.7%. The observed CBSFRC ultimate strains and strain energy densities ranged from 11.1–17.5 × 10⁻³ to 8.1–14.1 × 10⁵ J/m³, respectively. Matched-rate analysis showed that the largest DIF increment was 18.8% for SF-1CBRC at 40 s⁻¹, whereas the largest strain-energy-density increment was 70.2% for SF-1.5CBRC at 50 s⁻¹. These results describe the tested range and do not establish a universal optimum steel-fiber content. Full article

Boar sperm freezability (SF) is an economically important trait that influences reproductive efficiency and genetic improvement in pigs. However, its genetic basis remains poorly understood. In this study, semen samples from 382 Duroc and 151 Yorkshire boars were evaluated for sperm motility and recovery rate, and all individuals were genotyped using an 80K SNP array. Within-breed GWAS were first conducted, followed by a meta-analysis integrating the GWAS results from both boars. Individuals were classified into GSF and PSF groups based on sperm recovery rate for subsequent selection signature analysis. The results showed that Duroc boars exhibited significantly higher SF than Yorkshire boars. Heritability estimates for SF were moderate, with values of 0.35 in Duroc and 0.30 in Yorkshire. GWAS identified 10 significant SNPs in Duroc and 33 in Yorkshire associated with SF. Meta-analysis further detected 12 significant SNPs, annotated to candidate genes such as CCDC181, PARN, SOX9, and NCKAP5L. Association analysis identified ten representative variants significantly correlated with sperm recovery rate, with variants in PARN and MAP2K6 showing strong additive effects. Selection signature analysis revealed multiple genomic regions under differential selection between GSF and PSF groups, identifying several candidate genes associated with SF. Functional enrichment analysis indicated that these genes are mainly involved in spermatogenesis, flagellar motility, cellular stress response, and cold adaptation pathways. Overall, this study provides novel insights into the genetic architecture of boar SF and identifies potential molecular markers for genetic improvement and functional genomic studies in pigs. Full article

Programmable hydrogel actuators represent an innovative group of adaptive soft matter systems, which are able to respond to external stimuli with controllable mechanical movements for biomedical and bioengineering purposes. Natural silk fibroin (SF) is known to be a peculiar biomaterial, since it can exhibit controllable β-sheet-induced structural transitions, hierarchical self-assemblies, high biocompatibility, and mechanical adaptability, thus representing an ideal candidate for the development of dynamic hydrogels. In contrast to earlier reviews which focused more on SF hydrogel synthesis or biomedical applications, this review presents a mechanism-based understanding of programmable bioactuation by carefully correlating molecular design, network formation, stimuli responsiveness, and macroscopic deformation. Recent developments in SF hydrogel actuators are critically compared in terms of actuation principles, deformation behaviors, response dynamics, mechanical robustness, and functionalization, noting the natural compromise between fast response, strength generation, and durability in such materials. Novel concepts like nanocomposite materials, bioinspired designs, shape memory systems, and 4D printing are described as efficient ways to improve programmable deformation and functionality in soft materials. In addition, the biomedical opportunities of responsive SF hydrogels in wound healing, drug delivery, tissue engineering, wearable biosensors, and soft robots are critically discussed in relation to existing barriers for translation into practice. Combining mechanistic understanding with the comparative assessment of the performance of hydrogels is a basis for developing a complete rationale for the design of the next generation of SF hydrogel actuators and smart shape deformations. Full article

Lettuce (Lactuca sativa L.) is a widely consumed leafy vegetable cultivated globally, particularly under soilless systems to enable intensive and high-efficiency production. In these systems, precise nutrient solution management is essential, especially regarding calcium, an indispensable macronutrient involved in cell wall stabilization, membrane integrity, root development, and physiological responses to stress. This study evaluated the morphological and nutritional performance of crisp lettuce cultivars Mônica SF 31 and Veneranda grown using a nutrient film technique (NFT) system, using Calcário, Lithocal^®, and LT Supra^® as alternative calcium sources, alongside a control treatment. The experiment followed a randomized block design in a 4 × 2 factorial arrangement. Measured variables included root, leaf, and stem dry mass; stem length and diameter; and nutrient accumulation and gas exchange parameters. Data were subjected to analysis of variance and means were compared using Scott-knott test. Calcium supplementation significantly increased plant growth, with leaf fresh mass rising by 37% and root fresh mass by 56% compared to the control. Foliar calcium and magnesium concentrations were effectively enhanced by the treatments. Calcário, Lithocal^®, and LT Supra^® demonstrated effectiveness as calcium sources in soilless lettuce cultivation, promoting improved nutrient accumulation—particularly calcium and magnesium—and substantial gains in fresh biomass and root system development. Calcium supplementation is therefore recommended as a strategy to enhance productivity and crop quality in intensive soilless systems. Full article

Background/Objectives: Natural clinoptilolite–amoxicillin hybrids (CNZ@AMOX) were incorporated into alginate microcapsules via ionic gelation to develop a hybrid mineral–polymer delivery system for the controlled release of amoxicillin. Methods: A face-centered central composite design combined with response surface methodology (FCCD-RSM) was utilized to assess the effects of the zeolite/sodium alginate ratio, alginate concentration, calcium chloride concentration and curing time on the encapsulation efficiency (EE), sphericity factor (SF), and roundness (Rn). Results: The EE ranged from 5.9% to 91.3%, depending on the formulation composition. Numerical optimization identified the optimal conditions as 70.962% EE, 0.05 SF and 1.00 Rn, with a desirability score of 0.873. The incorporation of natural clinoptilolite improved microcapsule structural integrity and reduced the initial burst release by modulating diffusion pathways within the hybrid matrix. The optimized CNZ@AMOX exhibited pH-dependent release behavior, with minimal drug release in simulated gastric fluid (SGF) and diffusion-controlled release in simulated intestinal fluid (SIF), which was best described by the Korsmeyer–Peppas model. Conclusions: These findings demonstrate that zeolite–alginate hybrid microcapsules represent promising inorganic–organic composite carriers for the pH-responsive and controlled delivery of AMOX. Full article

The alkaline hydrolysis process is a promising method for treating SF-3 double-base propellant waste. This study aimed to validate the SF-3 waste alkaline hydrolysis reaction model and optimize its reaction conditions, thereby promoting the industrial application of alkaline hydrolysis treatment of double-base propellant wastes. Thus, the SF-3 alkaline hydrolysis reaction model was developed based on theoretical assumptions, derivations, and experimental validation. A central composite design coupled with response surface methodology was used to optimize the operational conditions, including reaction temperature, liquid–solid ratio, and reaction time. The results showed that the SF-3 alkaline hydrolysis conformed to a shrinking particle reaction model. The relationship between the x_SF and the t is x_SF = [(α + 2)t/(αt_e′) − 2t²/(αt_e′²)] × 100, where the limiting step is the alkaline hydrolysis process on the particle surface. In addition to reaction temperature and reaction time, the liquid–solid ratio influenced the SF-3 alkaline hydrolysis process. Furthermore, the results of Pareto and analysis of variance analyses revealed that the regression model equation was obtained as x_SF = 48.91 + 8.32₁ + 13.11X₃ − 3.49X₁² − 2.162X₂² − 4.179X₃² + 2.78X₁X₂. Meanwhile, the optimal reaction temperature, time, and liquid–solid ratio were 86.5 °C, 70.8 min, and 6.0, respectively. Under the optimal reaction conditions, the predicted x_SF using the regression model was 99.80%, while the experimental value was 95.01 ± 1.87%, demonstrating the accuracy of the regression model. Therefore, this study provides theoretical and technical support for the industrial-scale safe disposal and resource utilization of waste double-base propellants. Full article

Background: Lumbar foraminal canal stenosis (LFS) is typically multifactorial: disk height loss and bulging, facet hypertrophy/osteophytes, ligamentous thickening, and post-inflammatory or post-interventional scarring. Methods: To develop and implement a structured system for surgical care in chronic vertebrogenic pain with radicular features attributable to LFS, integrating population-based MRI morphometrics and interventional therapy response profiling, we conducted a single-center, multi-cohort observational study to develop, operationalize, and internally evaluate a quantitative, volume-integrated diagnostic and treatment stratification framework for LFS. Results: A retrospective evaluation of 351 surgically treated patients (2017–2023) was performed to identify structural and clinical drivers of persistent or recurrent pain. Following selective blockades and radiofrequency denervation, radiculopathy regressed in 44.7% of patients, facet-mediated pain improved in 44.0%, and the median pain relief duration was 3–6 months. Oswestry Disability Index (ODI) dynamics after interventional therapy showed significant improvement at 6 months (all p < 0.001). Both groups demonstrated significant improvement in VAS, ODI, and SF-36 scores over time. Endoscopic decompression achieved faster early leg pain relief (VAS leg, p < 0.001 at 6 and 12 months). ALIF resulted in superior long-term back pain control (VAS back, p < 0.001 at 12 and 24 months). At 24 months, pain levels were clinically equivalent between groups, indicating that procedure selection influences pain profile rather than absolute outcome. Conclusions: By integrating quantitative foraminal volume, nerve occupancy, and segmental stability, we demonstrated that treatment success in LFS is not determined by the magnitude of decompression alone, but by the precision of phenotypic matching. Indirect decompression, endoscopic foraminotomy, microsurgical decompression, and fusion-based stabilization each have a rational role when aligned with the biomechanical context rather than applied reflexively. Full article

Polylactic acid (PLA)-curcumin (CCM) composites, incorporating various contents of surface-functionalized dual-metal-doped copper oxide (SF-M-CuO), were prepared by the solution casting method. Synthesized composite films were evaluated for their antioxidant, biocompatible, photothermal, and antibacterial properties. The 4% CCM exhibits excellent compatibility based on total color difference, antioxidant activity, and controlled curcumin release behavior. In addition, different contents of SF-M-CuO (1–4%) were added to the PLA-4%-CCM polymer matrix. Synthesized composite films were characterized through functional, structural, and topographical analyses. FTIR and XRD analyses confirmed the successful incorporation of CCM and SF-M-CuO into the PLA matrix, which enhanced interfacial interactions and increased the crystallinity index by acting as effective nucleating agents. ABTS and DPPH radical scavenging assays revealed dose-dependent antioxidant activity due to the synergistic effects of CCM and SF-M-CuO. Biocompatibility evaluation using RAW 264.7 macrophage cells demonstrated non-toxic responses and enhanced cell proliferation in PLA-4%-CCM composite films containing up to 3%-SF-M-CuO. Among the fabricated films, PLA-4%-CCM-3%-SF-M-CuO exhibited superior photothermal performance and excellent antibacterial activity against Staphylococcus aureus and Escherichia coli, reducing bacterial counts to below the limit of detection. These findings demonstrate the potential of PLA-4%-CCM-3%-SF-M-CuO composite films as sustainable multifunctional materials for food safety and biomedical applications. Full article

Background: Adolescents exposed to large-scale natural disasters are at increased risk for psychological distress, yet individual psychological characteristics associated with stress and trauma responses remain insufficiently explored. Self-compassion has been suggested as a relevant factor in stress regulation and emotional adjustment. This study aimed to examine the associations between self-compassion, perceived stress, and trauma-related reactions in adolescents exposed to an earthquake. Methods: The sample consisted of 3362 adolescents (57.3% female) aged 14–17 years (mean age = 15.01 ± 0.97 years) who were exposed to the 2020 Elazığ–Sivrice earthquake. Participants completed the Perceived Stress Scale (PSS), the Self-Compassion Scale–Short Form (SCS-SF), and the Child Post-Traumatic Stress Reaction Index (CPTS-RI). Group comparisons and correlation analyses were conducted to examine relationships among the study variables and earthquake-related characteristics. Results: Adolescents residing in severely and moderately damaged areas reported significantly higher perceived stress and lower self-compassion compared to those in mildly damaged areas. Self-compassion was negatively correlated with trauma-related symptoms, whereas perceived stress showed a positive association with trauma reactions. Female adolescents reported higher perceived stress and trauma-related symptoms and lower self-compassion than males. Conclusions: The findings underscore the relevance of self-compassion in understanding adolescents’ psychological responses to earthquake-related stress and trauma and suggest that self-compassion may be considered in post-disaster psychological support efforts. Full article

The present study compares two scaffold cross-linking strategies, EDC/NHS and pentasodium triphosphate (TPP), applied to nanohydroxyapatite/silk fibroin/chitosan (nHA/SF/CTS) scaffolds. The effects of the cross-linking strategy on scaffold morphology, ATR-FTIR profile, swelling, in vitro degradation, and compressive properties were evaluated. In addition, the biological response of selected 20:40:40 and 15:70:15 (w/w) scaffold variants was assessed using SaOS-2 cells. The IR spectra of TPP- and EDC/NHS-cross-linked materials were qualitatively similar, and no distinct bands attributable to residual EDC or NHS were observed after washing. SEM observations indicated that the TPP-cross-linked scaffolds tended to have smaller and more spherical pores, whereas the EDC/NHS-cross-linked materials showed a more elongated pore morphology. These qualitative morphological differences were accompanied by differences in swelling, degradation, and low-strain mechanical response. The 20:40:40 formulation cross-linked with EDC/NHS showed the most favorable low-strain response, whereas PBS swelling markedly reduced the early compressive response of all scaffolds. Biological assessment showed that the 20:40:40 scaffolds supported viable SaOS-2 populations and maintained osteogenic activity, although cell colonization remained lower than on the control surfaces. The 15:70:15 variant performed less favorably, particularly after TPP cross-linking. These findings indicate that both cross-linking strategies can be used to obtain nHA/SF/CTS scaffolds, but their effects depend strongly on scaffold composition and on the property being considered. Full article

Feed intake and diet quality are key factors influencing enteric methane (CH₄) emissions in ruminants. Low-quality C₄ grasses typically limit intake and are associated with high CH₄ yield. Nitrogen supplementation may improve rumen function and reduce CH₄ emissions per unit of feed intake, although responses under low-quality forage conditions remain insufficiently characterized. The goal of the study was to evaluate the effects of nitrogen supplementation (urea- or nitrate-containing supplements) on the utilization of low-quality weeping lovegrass hay (Eragrostis curvula) and CH₄ yield in beef steers. Twenty-four Aberdeen Angus steers (326 ± 27 kg body weight) were assigned to three treatments: (1) weeping lovegrass hay alone; (2) weeping lovegrass hay + sunflower expeller + urea; and (3) weeping lovegrass hay + sunflower expeller + potassium nitrate (KNO₃). The proportion of non-protein nitrogen (NPN; urea and KNO₃) included in the supplements was set according to the maximum tolerated threshold. Methane emissions were measured using the SF₆ tracer technique. Compared with the hay-only treatment, supplemented animals increased dry matter intake (DMI) by 35% and 38% in the urea and nitrate treatments, respectively (p < 0.01). Total CH₄ emissions (g/d) were not affected by treatment (p = 0.16). However, CH₄ yield (g CH₄/kg DMI) decreased by 27% and 38% in the urea and nitrate treatments, respectively (p < 0.01). The methane conversion factor (Ym) was also reduced in supplemented animals. Under the conditions of this study, supplementation of low-quality weeping lovegrass hay with nitrogen-containing supplements increased feed intake and reduced CH₄ yield without affecting total CH₄ emissions. These findings highlight the importance of considering CH₄ emission intensity, in addition to absolute emissions, when evaluating mitigation opportunities in forage-based beef production systems. Full article

As augmented reality head-up display (AR-HUD) becomes increasingly integrated into intelligent vehicles, inappropriate interface designs may increase drivers’ cognitive workload and delay hazard responses. This study investigates how cognitive style, driving task type, and AR-HUD navigation design jointly influence drivers’ behavioral performance and visual attention. A total of 55 participants were recruited and screened using the Group Embedded Figures Test, with 38 drivers finally selected for a 2 × 4 × 2 driving-simulation experiment comparing world-fixed (WF) and screen-fixed (SF) interfaces across goal-directed and stimulus-driven tasks. Reaction times and eye-tracking indicators were analyzed using generalized linear models. Results show that stimulus-driven tasks significantly increased reaction times, with rear-vehicle scenarios producing the longest responses (mean = 1.420). During lane-change tasks, WF displays significantly reduced fixation duration (p < 0.001) and fixation counts (p < 0.001), whereas SF displays improved attentional efficiency during pedestrian-warning tasks. In addition, field-dependent drivers exhibited significantly larger pupil diameters, indicating higher cognitive workload. These findings provide sensor-based evidence for AR-HUD systems that dynamically optimize interface presentation according to task context and workload conditions. Full article

Neurodegenerative disorders, including Parkinson’s and Alzheimer’s diseases, are hallmarked by the progressive degeneration of neuronal networks. Given the lack of disease-modifying cures, current therapies are limited to symptomatic relief. Here, we investigated the neurotrophic potential of the skin secretion (SS) from Rhinella schneideri, its polar fraction (PF) and nonpolar (NPF) fraction, and respective subfractions on the morphology of neuron-like cells. Following initial H₂O-CH₂CL₂ partitioning, PF and NPF subfractions were isolated via RP-HPLC. Chemical characterization using LC-MS-IT-TOF identified eight distinct molecules, notably bufotenine and marinobufagin. Cytotoxicity screening established safe working concentrations (100–250 ng/mL for SS/PF; 250–500 ng/mL for NPF and subfractions) for downstream morphological evaluations using High Content Screening (HCS). The subfraction polar 5 (SfP5) elicited a robust neurotrophic response, significantly enhancing all assessed morphometric parameters: total neurite outgrowth (+72%), branching points (+120%), maximum process length (+60%), and total number of processes (+35%). Our data show that Rhinella schneideri SS contains molecules that improve in vitro neuronal networks, serving as a promising source for preliminary screening of neuroprotective effects. Full article

LGBTQ+ individuals experience disparities in cardiovascular health, but little is known about how daily minority and general stress affect physiological and psychological responses in real-world settings. Twenty LGBTQ+ young adults aged 18–27 completed a 14-day exploratory pilot study using ecological momentary assessment (EMA) with four daily smartphone surveys and continuous smartwatch-based sensor monitoring. This study is among the first to combine EMA with wearable sensor data to capture autonomic stress responses to minority stressors in naturalistic settings. Outcomes included a physiological stress score derived from heart rate variability during the 60 min before each EMA completion, as well as positive and negative affect (PA and NA). Four stress measures, Everyday Discrimination Scale (EDS), Sexual Orientation Microaggression Inventory Short Form (SOMI-SF), EMA of stressful events (EMA-SE), and current perceived stress (CPS), and a combined variable (COMB) were examined. In mixed-effects within-person models, all stress measures showed trends in the expected direction, with higher physiological stress scores, lower PA, and higher NA, though these varied in magnitude and statistical significance. SOMI-SF showed the strongest association with physiological stress, while general stress measures showed stronger associations with affect. These preliminary findings suggest that LGBTQ+-specific and general stressors may differentially engage physiological and psychological response systems; however, caution is warranted given the small sample size. Full article