Search Results (5,913)

Osteoarthritis is a common cause of equine lameness and can involve periarticular osteophytes and subchondral bone (SB) changes. This exploratory ex vivo study evaluated the diagnostic performance of digital tomosynthesis (DT) for detecting these findings in the distal interphalangeal and metacarpophalangeal joints, using computed tomography (CT) classifications as the reference. Sixteen distal forelimbs from nine adult horses underwent imaging with both modalities under simulated weight-bearing conditions. Three independent observers graded osteophytes and SB changes, and the scores were dichotomised as absent or present for analysis. The primary analysis used a majority-based CT reference, defined by agreement of at least two observers. Observer-specific comparisons and within-observer DT–CT agreement were evaluated in a secondary analysis. In the primary analysis, sensitivity ranged from 23.1% (95% CI 9.5–39.3%) to 34.6% (95% CI 13.8–56.0%), specificity from 94.1% to 96.1%, Youden’s index from 19.2% to 30.7%, and Cohen’s κ from 0.249 to 0.377. Sensitivity was numerically higher for osteophytes than for SB changes for all three observers, without formal comparison. DT missed many CT-positive assessments but produced few false-positive classifications. Studies in live horses comparing DT with conventional radiography are needed to determine its additional diagnostic value. Full article

Soft actuators capable of flexible motion have been actively studied and developed. Soft actuators that can extend and bend have been developed; however, soft rotary actuators have rarely been reported. In our previous studies, a soft rotary actuator (SRA) that generates rotational motion by constraining extension-type soft actuators (EFPAs) in a spiral shape was developed. However, the SRA had the limitation of rotating only in one direction, either clockwise or counterclockwise. In this paper, we aim to develop a symmetric bidirectional soft rotary actuator (SB-SRA) that achieves comparable rotational performance in both clockwise and counterclockwise directions while maintaining the flexibility of polymeric materials, such as rubber, and allowing static hysteresis adjustment. In particular, arranging several EFPAs so that they do not interfere with each other during extension enables the SRA to generate both clockwise and counterclockwise rotational motion. Unlike previously reported unidirectional soft rotary actuators, and unlike rigid antagonistic bidirectional joints that require bearings and a dedicated brake, the proposed SB-SRA achieves symmetric bidirectional rotation with a bendable rotational axis and enables static hysteresis compensation using only the opposing actuators. The results show that the maximum rotation angle and torque of the SB-SRA are approximately 105° and 0.51 Nm, respectively, under an input pressure of 500 kPa. The maximum directional differences between clockwise and counterclockwise operation were 3.3% for the rotation angle and 2.4% for the torque, demonstrating approximately symmetric output characteristics. In addition, static hysteresis adjustment can be achieved by varying the supply pressures applied to the opposing EFPAs. Full article

Effective bonding between zirconia ceramic restorations and resin remains a clinical challenge, particularly in the context of intraoral repair and cementation. This study aimed to investigate the micro-shear bond strength (μSBS) of stereolithography (SLA)- and digital light processing (DLP)-fabricated zirconia–resin cement-composite resin bonding specimens. Rectangular zirconia specimens (10 × 5 × 5 mm³) were fabricated by SLA, DLP, and computer numerical control (CNC) milling technologies. The surface roughness (Ra) of zirconia was measured after air abrasion. A composite resin cylinder (5 mm in height and 1 mm in diameter) was added to each zirconia substrate for the μSBS test. The failure mode images were obtained using a scanning electron microscope. The adhesive, cohesive, and mixed failure patterns were analyzed. The Ra and μSBS values were statistically analyzed with one-way ANOVA followed by S-N-K post hoc comparisons (a = 0.05). The Ra of SLA (1.05 ± 0.03 μm) and DLP (1.02 ± 0.03 μm) zirconia after air-abrasion were significantly higher than that of CNC (0.91 ± 0.02 μm) (p < 0.001). The SLA (23.37 ± 4.66 MPa) and DLP (23.24 ± 3.7 MPa) groups exhibited similar μSBS values for the bonding specimens to those of the CNC group (21.13 ± 3.48 MPa) (p = 0.237), indicating that the bond strength of the zirconia–resin cement-composite resin assembly for SLA and DLP zirconia was comparable to that for CNC zirconia. Full article

Objective: Esophageal cancer (ESCA) has poor prognosis and lacks reliable biomarkers. This study aimed to construct a mitochondrial energy metabolism-related prognostic model and identify key regulatory genes. Methods: TCGA-ESCA and GSE53625 data were used as training and validation cohorts, respectively. Mitochondrial energy metabolism scores were calculated by ssGSEA. Differential expression analysis and WGCNA identified key genes, followed by consensus clustering, Cox/LASSO regression, enrichment analysis, immune infiltration, TIDE evaluation, and nomogram development. INHBA knockdown or overexpression was performed in KYSE-150 and TE-1 cells to assess malignant phenotypes, mitochondrial function, EMT markers, and Smad2/3 signaling, with SB431542 used for pathway blockade. Results: High mitochondrial energy metabolism scores predicted poorer overall survival. A total of 124 key genes were identified, and three molecular subtypes were established, with Cluster 2 showing the worst prognosis. A four-gene signature comprising COL11A1, INHBA, TNFAIP6, and POSTN stratified patients into high- and low-risk groups in both cohorts. High-risk tumors were enriched in extracellular matrix remodeling, cell adhesion, inflammatory response, hypoxia, angiogenesis, and PI3K-Akt signaling, whereas oxidative phosphorylation was more active in low-risk tumors. The high-risk group had higher TIDE scores, suggesting poorer immunotherapy response. The nomogram incorporating risk group and M stage showed limited discrimination after bootstrap correction. In vitro, INHBA promoted proliferation, migration, invasion, EMT, mitochondrial membrane potential, ATP production, and oxidative phosphorylation. SB431542 partially reversed these effects. Conclusions: This MEMRG-based model predicts ESCA prognosis and immune features. INHBA promotes ESCA progression by activating Smad2/3-mediated EMT and mitochondrial metabolic reprogramming, suggesting its potential as a prognostic biomarker and therapeutic target. Full article

Background/Objectives: Although polyetheretherketone (PEEK) has been widely used for its excellent properties, its chemical inertness remains an ongoing challenge. This study aims to evaluate the effects of corona discharge (CD) treatment on the adhesion of dental PEEK to resin cement. Methods: Mirror-polished (MP) or sandblasted (SB) PEEK specimens were divided into CD-treated [CD(+)] and -untreated [CD(−)] groups. Following primer application, the specimens were bonded with resin cement and subjected to either 0 (TC0) or 5000 (TC5000) thermal cycles. The tensile bond strength (TBS) was measured and statistically analyzed using one-way ANOVA and t-tests (α = 0.05). Failure modes were analyzed and classified as adhesive, mixed, or cohesive. The surface roughness and contact angle were also evaluated. Results: CD treatment increased the surface roughness of the MP specimens while slightly decreasing that of the SB specimens. The wettability of both surfaces was significantly improved after CD treatment. However, no significant differences in TBS between the CD(−) and CD(+) groups were observed for either the MP or SB specimens. No significant differences in TBS were observed under different TC durations, regardless of CD treatment. Conclusions: Although CD treatment significantly improved the surface roughness and wettability of dental PEEK, it did not enhance the TBS of either the MP or SB surfaces. Full article

Background: Short bowel syndrome (SBS) secondary to intestinal resection is the most common cause of intestinal failure (IF) in children and is associated with prolonged parenteral support (PS), impaired growth, and substantial morbidity. SBS outcomes have improved dramatically over the past ten years due to advancements in intestinal rehabilitation programs, nutritional approaches, pharmaceutical treatments, and surgical restoration. Objective: With an emphasis on surgical decision-making, intestinal rehabilitation, nutritional optimization, pharmacologic advancements and predictors of enteral autonomy, the goal is to thoroughly review and synthesize the most recent data on managing pediatric SBS after intestinal resection. Methods: This systematic review was conducted in accordance with the PRISMA 2020 statement using PubMed and Embase. Eligible primary clinical studies evaluated management strategies or clinically relevant outcomes in pediatric patients with SBS secondary to intestinal resection. Secondary evidence sources, including review articles and evidence-based position papers, were considered separately and were used to contextualize the primary findings and contemporary clinical recommendations. The methodological quality and risk of bias of primary studies were assessed using design-appropriate critical appraisal tools. Owing to substantial clinical and methodological heterogeneity, the evidence was synthesized qualitatively. Results: Multidisciplinary intestinal rehabilitation programs have become the mainstay of therapy, increasing the rates of enteral autonomy and improving survival. While optimal PS administration reduces intestinal failure–associated liver disease (IFALD) and catheter-related bloodstream infections (CRBSIs), early, organized enteral feeding encourages intestinal adaptation. Significant advances in decreasing PS requirements have been achieved by pharmacological therapies such as glucagon-like peptide-2 (GLP-2) analogs. Advances in multidisciplinary intestinal rehabilitation have reduced the need for intestinal transplantation and have refined patient selection for autologous bowel reconstruction. Consequently, procedures such as Serial Transverse Enteroplasty (STEP) and Longitudinal Intestinal Lengthening and Tailoring (LILT) are now reserved for highly selected patients with persistent bowel dilatation, dysmotility, and inadequate intestinal adaptation despite optimized nutritional and medical therapy. Conclusions: Following intestinal resection, pediatric SBS is now managed through multidisciplinary, protocol-driven intestinal rehabilitation. Outcomes have improved through the integration of nutritional, pharmacological, and surgical strategies tailored to each patient’s residual anatomy and adaptive potential. Future prospective pediatric studies are needed to standardize outcome measures and clarify the optimal timing and sequencing of nutritional, pharmacological, and surgical interventions during intestinal rehabilitation. Full article

Transposon integration is influenced by genomic context, but the contribution of three-dimensional genome organization to transposon mobility remains poorly understood. Here, we developed T7-Mediated Unique-barcode Recovery sequencing (TMUR-seq), combining T7-based junction enrichment with uniquely barcoded Sleeping Beauty (SB) transposons to track secondary transposition events and their relationships to individual donor sites. Using this approach, we characterized genome-wide secondary SB transposition in human cells and analyzed the effects of genomic distance, donor-specific directionality, and chromatin organization on target selection. Secondary transposition was strongly dependent on linear genomic distance but showed pronounced donor-specific directional asymmetries, including recurrent mirror-image patterns at neighboring donor sites. Secondary integratiotes also showed enrichment for Hi-C contacts with their donor loci within approximately 50 kb after accounting for genomic distance, consistent with a contribution of local three-dimensional chromatin organization to target selection. An extended chromosome 2 donor cluster further illustrated how donor distribution, chromatin state, domain organization, and local three-dimensional connectivity can coincide with highly asymmetric integration patterns and regions of pronounced integration depletion. Together, these findings indicate that secondary SB transposition is not entirely random and is shaped by the combined effects of genomic distance and local genomic context. TMUR-seq provides a framework for tracking donor–recipient relationships and investigating the genomic determinants of transposon mobility. Full article

Asphalt binders are complex viscoelastic polymeric materials whose rheological behavior is governed by interactions among their chemical constituents and, in polymer-modified systems, the morphology and stability of the dispersed polymer network. This study developed a performance-based framework for selecting the type and dosage of bio-based soybean recycling agents (RAs) for styrene-butadiene-styrene (SBS)-modified asphalt mixtures containing 30% reclaimed asphalt pavement (RAP). Ten dense-graded asphalt mixtures with a nominal maximum aggregate size of 12.5 mm were evaluated, including a control mixture produced with PG 76-22 (PG 67-22 asphalt binder modified with 3.5% SBS) and no RAP, and nine mixtures containing 30% RAP and three soybean-based recycling agents (RA1, RA2, and RA3) at dosages of 0.5%, 2.0%, and 4.0% by weight of binder. Binder characterization included Superpave performance grading, while mixture performance was evaluated using the Hamburg Wheel Tracking (HWT) test, freeze–thaw-conditioned HWT, Semi-Circular Bend (SCB), IDEAL-CT, IDEAL-RT, and Cantabro abrasion loss tests. Increasing recycling-agent dosage improved cracking resistance but progressively reduced rutting resistance, consistent with the measured decrease in binder viscosity. The magnitude of the softening effect followed the order RA3 > RA2 > RA1, consistent with measured viscosity reductions and HWT rut-depth response. Integrating SCB fracture resistance with the HWT rutting criterion identified acceptable dosage ranges of 1.4–4.0%, 0.5–2.8%, and 0.5–0.7% for RA1, RA2, and RA3, respectively. These results demonstrate that the effectiveness of the evaluated recycling agents, as reflected by binder rheology and asphalt mixture performance, influences the balance between fracture resistance and permanent deformation, providing a performance-based framework for optimizing bio-based recycling-agent selection in high-RAP polymer-modified asphalt mixtures. Full article

Purslane (Portulaca oleracea L.) is a nutritious leafy vegetable rich in phenolic compounds, vitamins, minerals, and dietary fiber. However, pesticide residues remaining after cultivation may pose risks to human health, making effective post-harvest decontamination treatments necessary. This study investigated the effects of different washing treatments 5% sodium bicarbonate (SB), 5% acetic acid (AA), 5% citric acid (CA), a mixture containing 2.5% acetic acid and 2.5% citric acid (AA-CA), tap water washing (TW), and ultrasound-assisted washing for 5, 10, and 15 min (US-5′, US-10′, and US-15′, respectively) on pesticide residue reduction and quality characteristics of purslane. This study used an artificial surface contamination model with a 4 h holding period before washing. Residue analyses of acetamiprid, cypermethrin, imidacloprid, and propargite were performed by LC-MS/MS and GC-MS/MS using the QuEChERS extraction method. The antioxidant capacity and total phenolic content of the SB sample were significantly lower than those of the other treatments. Color analysis showed significantly higher ΔE* values in the SB sample. Acidic treatments generally resulted in higher TA% and lower pH and chlorophyll contents than the other treatments. Depending on the pesticide and washing treatment, residue reduction efficiencies ranged from 6.33% to 72.22%. Ultrasound-assisted washing (UAW) provided a favorable balance between residue reduction and preservation of several quality attributes, including total phenolic content (TPC), antioxidant capacity, total flavonoid content (TFC), color, and chlorophyll contents in purslane. These results indicate that ultrasound-assisted washing, particularly the 5 min treatment, provided a favorable balance between pesticide residue reduction and preservation of several quality attributes; however, longer ultrasound exposure was associated with deterioration of some quality characteristics, particularly firmness and weight loss. Full article

Highly integrated photonic devices have attracted considerable attention for data-transmission systems. However, conventional metal-based photonic devices provide limited tunability and control over electromagnetic surface waves. Therefore, dynamically tunable integrated photonic devices are needed. This paper presents a theoretical model of an indium antimonide–uniaxial chiral–indium antimonide (InSb-UAC-InSb) structure operating in the near-infrared frequency regime. Electromagnetic wave theory is used for numerical analysis, and the characteristic equation is obtained by applying the appropriate boundary conditions. The propagation constant is examined for different values of chirality, core-width, InSb temperature, and incident-wave frequency for two types of uniaxial chiral media. Variation in temperature and chirality demonstrate the tunability of the interface under different operating conditions, enabling enhanced light confinement and low-loss propagation modes in the near-infrared region. The results show that the propagation constant in Case II exhibits greater sensitivity than that in Case I and shows a high value even at lower frequencies. This study provides a promising platform for thermally reconfigurable photonic components, temperature-sensitive optical devices, and near-infrared optical communication applications. Full article

Background/Objectives: Juvenile myelomonocytic leukemia (JMML) is a rare and very aggressive pediatric myelodysplastic/myeloproliferative neoplasm with molecular heterogeneity and constitutive activation of the RAS signaling pathway. The aim of this study was to identify the mutational landscape, driver genes, mutational signatures, functional pathways, and therapeutic targets of mutations that affect receptor tyrosine kinases (RTKs) and RAS pathways in JMML. Methods: We collected tumor and matched buccal swab samples from 35 patients with JMML and performed whole-exome sequencing. Variants were called using GATK-Mutect2 and annotated with ANNOVAR. Mutational profiling, co-occurrence analysis, protein domain mapping, and driver gene identification were performed using maftools and OncodriveCLUST. DGIdb was used to explore drug–gene interactions, MutationalPatterns was used for the characterization of mutational signatures, and the clusterProfiler package was used for the functional enrichment analyses. Results: Alteration of the RTK-RAS pathway was found in 77.1% of patients. PTPN11 (23%), NRAS (20%), and KRAS (14%) were the most frequently altered genes, followed by PTEN (11%) and FLT3, ROS1, FGFR4, ERBB2, and EGFR (9% each). Domain mapping of protein sequences identified the grouping of mutations within the conserved functional domains of the proteins. The alterations in receptor tyrosine kinase genes demonstrated a significant degree of co-occurrence, while the alterations in canonical JMML genes showed mutual exclusivity. PTPN11, NRAS, ROS1, and FGFR4 were top driver genes based on driver gene analysis. Functional enrichment analysis revealed significant enrichment of receptor tyrosine kinase signaling, Ras/MAPK and PI3K-AKT pathways. The mutational signatures were mostly SBS5-like, with enrichment of C>T transitions, indicative of endogenous mutational processes. Drug–gene interaction analysis identified EGFR, ALK, ROS1, ERBB2, FLT3, FGFR4, and PDGFRA as highly interconnected and potentially actionable therapeutic targets. Conclusions: These findings expand the molecular landscape of JMML and underscore the importance of aberrant RTK-RAS signaling in disease pathogenesis. The identification of recurrent and potentially druggable RTK alterations provides a rationale for future precision medicine approaches in JMML. Full article

The burgeoning global demand for rare earth elements (REEs), driven by rapid technological advancements, has elevated their strategic significance. As a ubiquitous metallic mineral that crystallizes throughout the magmatic and hydrothermal stages, pyrite can potentially record the history of REE enrichment within magmatic–hydrothermal systems. The Bachu carbonatite-hosted REE deposit in Xinjiang represents a significant rare earth resource base in China. Elucidating its ore-forming mechanism is therefore crucial for understanding regional metallogenesis and guiding future exploration efforts. In this study, pyrite from this deposit is investigated in detail by employing an integrated analytical approach that includes scanning electron microscopy (SEM), electron probe microanalysis (EPMA), laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) trace-element analysis, and in situ sulfur isotope analysis. Based on textural and petrogenetic relationships observed under microscopy, three distinct types of pyrite are identified: (1) pyrite coexisting with hydrothermal minerals such as barite and celestite; (2) pyrite showing evident replacement textures, despite the absence of direct intergrowth with sulfate minerals; and (3) pyrite displaying neither intergrowth nor replacement textures with sulfates. These textural distinctions, combined with variations in Co/Ni ratios, Co-Ni-As ternary plots, and Co/Sb vs. Se/As systematics, enable a clear discrimination between pyrite formed during the magmatic (Py1) and hydrothermal (Py2) stages. Pyrite from the magmatic stage (Py1) is characterized by positive δ³⁴S values ranging from 0.03‰ to 4.67‰, with a pronounced peak at 1.30‰, which is higher than the mantle δ³⁴S value (~0‰). This sulfur isotope signature suggests that crustal material was involved in the petrogenesis of the Bachu carbonatite. In contrast, pyrite from the hydrothermal stage (Py2) exhibits a bimodal distribution of δ³⁴S values. One group, peaking at approximately +0.93‰, indicates a magmatic sulfur source genetically related to Py1. The other group, however, displays distinctly negative values, with a peak around −4.93‰, which may be attributed to sulfur isotope fractionation processes during mineralization. Notably, barite from the hydrothermal stage exhibits consistently positive and elevated δ³⁴S values, ranging from 10.46‰ to 15.94‰ (average 12.82‰). The contrasting δ³⁴S values compared to the negative Py2 values manifest a clear “Tower Effect,” strongly suggesting that the extensive precipitation of barite was a primary driver of the negative sulfur isotope values observed in the composition of coexisting hydrothermal pyrite (Py2). The pyrite generations also establish a stage-specific link to REE mineralization: Py1 records the reduced magmatic interval of carbonatite differentiation and primary REE preconcentration, whereas Py2 is associated with the barite–celestine–fluorapatite–monazite assemblage of the REE-rich hydrothermal veins and records the later oxidized fluid overprint. By integrating detailed mineralogical and geochemical evidence, this study successfully distinguishes between magmatic and hydrothermal pyrite populations and provides critical constraints on the sources of ore-forming materials in the Bachu carbonatite-type REE deposit. Full article

Crude antimony containing 8–9 wt.% sodium and, in some cases, up to 25–26 wt.% sodium is obtained during the carbothermic reduction of sodium antimonate through crucible smelting. The boundaries of vapour–liquid equilibrium on the Sb–Na phase diagram were constructed to assess the possibility of using distillation processes to refine crude antimony from sodium. The boundaries of the liquid–vapour phase transitions were calculated at atmospheric pressure and under vacuum conditions (133 Pa). The presence of the incongruently evaporating compound Na₃Sb makes it possible to divide the binary Sb–Na system into two quasi-binary systems: Sb–Na₃Sb and Na₃Sb–Na. In this study, we established that the separation of antimony and sodium (the Sb–Na₃Sb system) using distillation at atmospheric pressure is technically difficult because of the high boiling temperatures of the melts in the Sb–Na₃Sb system (1635–2617 °C) and the very narrow temperature range of the vapour–liquid equilibrium field under vacuum. Molten sodium and trisodium antimonide (the Na₃Sb–Na system) can be separated both at atmospheric pressure and under vacuum when the sodium concentration in antimony exceeds 90 at.% Na (approximately 63 wt.%). However, the first case requires temperatures of up to 1570 °C, whereas vacuum conditions require a temperature of approximately 600 °C. Under these conditions, the vapour phase will consist of almost pure sodium, while antimony will accumulate in the still residue in the form of Na₃Sb. In this paper, we also present calculated thermodynamic functions, namely, the entropies and enthalpies of mixing and evaporation of antimony–sodium melts, which will supplement the existing database of physicochemical data. Full article

This in vitro study evaluated the shear bond strength (SBS) of resin cement to diabetic and non-diabetic dentin following immediate dentin sealing (IDS) or conventional bonding without IDS, under two cementation protocols: immediate cementation (IC) and one-week delayed cementation (DC) after provisionalization. From a biomimetic perspective, IDS represents a tissue-preserving adhesive approach aimed at maintaining a stable resin–dentin interface; however, systemic conditions such as diabetes mellitus may alter the dentin substrate and affect adhesive performance. A total of 120 extracted human molars were divided into diabetic (n = 60) and non-diabetic (n = 60) groups, each further categorized by adhesive strategy (IDS or without IDS) and cementation protocol (IC or DC). IDS was performed using a three-step etch-and-rinse adhesive system, and cementation was completed with a dual-cure resin cement. Differences in SBS were observed between diabetic and non-diabetic dentin, adhesive strategies, and cementation protocols. Across all conditions, IDS-treated specimens exhibited higher bond strength than those without IDS, and DC resulted in lower SBS than IC in both adhesive strategies. In all subgroups, non-diabetic dentin showed greater SBS than diabetic dentin. The highest SBS was recorded in the non-diabetic IDS-IC group, whereas the lowest was observed in the diabetic without IDS–DC group. Under the tested laboratory conditions, IDS-treated groups showed higher SBS than the corresponding groups without IDS, while the immediate cementation protocol resulted in higher SBS than the delayed cementation protocol. Full article

Skeletal muscle ageing is accompanied by impaired regeneration and remodelling of the multicellular niche, yet how age-related immune changes relate to altered muscle stem cell (MuSC) states in humans remains unclear. Here, we integrated bulk RNA-sequencing, scRNA-seq and an independent older adult cohort analysis to characterize age-associated changes in human skeletal muscle and identify candidate macrophage–MuSC signalling interactions. Bulk transcriptomic analysis revealed inflammatory activation, reduced mitochondrial and metabolic programmes, and increased macrophage-associated signatures in aged muscle. Single-cell analysis resolved distinct myeloid and MuSC states. Among aged myeloid populations, inflammatory macrophages exhibited the highest SenMayo score, whereas donor-level pseudobulk analysis identified age-related early-primed MuSCs (epMuSC) remodelling involving inflammatory, interferon-related, and extracellular matrix-associated programmes. Ligand–target modelling prioritized inhibin subunit beta A (INHBA)/Activin A as a candidate signal linking inflammatory macrophages to the aged epMuSC programme. This prediction was further supported by an independent older adult human muscle scRNA-seq cohort showing concordant INHBA expression in inflammatory macrophages and Activin receptor expression in epMuSCs. In primary human MuSCs, Activin A induced Activin-responsive genes and suppressed myogenic regulators, and these effects were attenuated by SB431542, a small-molecule inhibitor of ALK4/5/7 signalling. Together, these findings support a candidate INHBA-associated, cell state-resolved communication framework between inflammatory macrophages and epMuSC remodelling in aged human muscle, providing a potential link between immune remodelling and altered regenerative cell states. Full article