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24 pages, 2162 KiB  
Article
African Small Mammals (Macroscelidea and Rodentia) Housed at the National Museum of Natural History and Science (University of Lisbon, Portugal)
by Maria da Luz Mathias and Rita I. Monarca
Diversity 2025, 17(7), 485; https://doi.org/10.3390/d17070485 - 15 Jul 2025
Viewed by 208
Abstract
The National Museum of Natural History and Science holds a historical collection of 279 small African mammal specimens (Macroscelidea and Rodentia), representing 32 species, gathered during the Portuguese colonial period in Mozambique, Angola, and Guinea-Bissau. This study examines the collection, updates the small [...] Read more.
The National Museum of Natural History and Science holds a historical collection of 279 small African mammal specimens (Macroscelidea and Rodentia), representing 32 species, gathered during the Portuguese colonial period in Mozambique, Angola, and Guinea-Bissau. This study examines the collection, updates the small mammal species lists for each country, and highlights its importance as a historical baseline for biodiversity research. Rodents dominate the collection, reflecting their natural abundance and diversity, while Macroscelidea are less represented. The Angolan subset of the collection has the highest number of both specimens and species represented. Mozambique is underrepresented, and the Guinea-Bissau subset offers an extensive rodent representation of the country’s inventory. The most well-represented species are Gerbilliscus leucogaster, Lemniscomys striatus, Lemniscomys griselda (from Angola), and Heliosciurus gambianus (from Guinea-Bissau). Notably, the collection includes the neo-paratype of Dasymys nudipes (from Angola). Most species are common and not currently threatened, with geographic origin corresponding to savanna and forest habitats. These findings underscore the importance of integrating historical data and current biodiversity assessments to support multidisciplinary studies on target species, regions, or countries. In this context, the collection remains a valuable key resource for advanced research on African small mammals. Full article
(This article belongs to the Section Animal Diversity)
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15 pages, 2052 KiB  
Article
Assessment of Potential Environmental Risks Posed by Soils of a Deactivated Coal Mining Area in Northern Portugal—Impact of Arsenic and Antimony
by Marcus Monteiro, Patrícia Santos, Jorge Espinha Marques, Deolinda Flores, Manuel Azenha and José A. Ribeiro
Pollutants 2025, 5(2), 15; https://doi.org/10.3390/pollutants5020015 - 18 Jun 2025
Viewed by 843
Abstract
Active and abandoned mining sites are significant sources of heavy metals and metalloid pollution, leading to serious environmental issues. This study assessed the environmental risks posed by potentially toxic elements (PTEs), specifically arsenic (As) and antimony (Sb), in the Technosols (mining residues) of [...] Read more.
Active and abandoned mining sites are significant sources of heavy metals and metalloid pollution, leading to serious environmental issues. This study assessed the environmental risks posed by potentially toxic elements (PTEs), specifically arsenic (As) and antimony (Sb), in the Technosols (mining residues) of the former Pejão coal mine complex in Northern Portugal, a site impacted by forest wildfires in October 2017 that triggered underground combustion within the waste heaps. Our methodology involved determining the “pseudo-total” concentrations of As and Sb in the collected heap samples using microwave digestion with aqua regia (ISO 12914), followed by analysis using hydride generation-atomic absorption spectroscopy (HG-AAS). The concentrations of As an Sb ranging from 31.0 to 68.6 mg kg−1 and 4.8 to 8.3 mg kg−1, respectively, were found to be above the European background values reported in project FOREGS (11.6 mg kg−1 for As and 1.04 mg kg−1 for Sb) and Portuguese Environment Agency (APA) reference values for agricultural soils (11 mg kg−1 for As and 7.5 mg kg−1 for Sb), indicating significant enrichment of these PTEs. Based on average Igeo values, As contamination overall was classified as “unpolluted to moderately polluted” while Sb contamination was classified as “moderately polluted” in the waste pile samples and “unpolluted to moderately polluted” in the downhill soil samples. However, total PTE content alone is insufficient for a comprehensive environmental risk assessment. Therefore, further studies on As and Sb fractionation and speciation were conducted using the Shiowatana sequential extraction procedure (SEP). The results showed that As and Sb levels in the more mobile fractions were not significant. This suggests that the enrichment in the burned (BCW) and unburned (UCW) coal waste areas of the mine is likely due to the stockpiling of lithic fragments, primarily coals hosting arsenian pyrites and stibnite which largely traps these elements within its crystalline structure. The observed enrichment in downhill soils (DS) is attributed to mechanical weathering, rock fragment erosion, and transport processes. Given the strong association of these elements with solid phases, the risk of leaching into surface waters and aquifers is considered low. This work underscores the importance of a holistic approach to environmental risk assessment at former mining sites, contributing to the development of sustainable remediation strategies for long-term environmental protection. Full article
(This article belongs to the Section Soil Pollution)
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16 pages, 3358 KiB  
Article
The Influence of Forest Fires on Ecological, Economic, and Social Trends in Landscape Dynamics in Portugal
by Vasco Lopes, Luis Carreira dos Santos and Juan-M. Trillo-Santamaría
Land 2025, 14(6), 1273; https://doi.org/10.3390/land14061273 - 13 Jun 2025
Viewed by 415
Abstract
The Portuguese forest plays a crucial role in maintaining ecological balance and fostering socio-economic sustainability within rural areas. Nonetheless, it is currently facing significant challenges due to the increasing intensity and frequency of forest fires observed in recent decades. The deterioration of traditional [...] Read more.
The Portuguese forest plays a crucial role in maintaining ecological balance and fostering socio-economic sustainability within rural areas. Nonetheless, it is currently facing significant challenges due to the increasing intensity and frequency of forest fires observed in recent decades. The deterioration of traditional agricultural practices, the proliferation of monocultures, and alterations in land use patterns have significantly exacerbated these challenges. Consequently, the landscape has undergone considerable transformations, resulting in a decline in biodiversity and a weakening of local economies. This study examines land use in mainland Portugal from 1995 to 2018, utilising data on land occupation, land cover, and burnt areas from the Institute for Nature Conservation and Forests. The cartographic analysis of three periods—1995, 2007, and 2018—along with the fire data recorded between 1996 and 2018, enabled the observation of changes in the predominant land use and land cover (LULC) classes, particularly among forests, scrubland, and agricultural areas. The results highlight a significant increase in forested areas, especially eucalyptus, as well as urbanisation, while scrubland and agricultural areas have decreased. Using specific LULC level 4, and burnt (BA) and unburnt (NB) areas, temporary crops decreased substantially (−14% NB/−4% BA 1995–2007; −23% NB by 2018). Eucalyptus showed strong continuous growth (16% NB/35% BA 1995–2007; 23% NB/47% BA 2007–2018). Maritime pine suffered severe losses, especially in burnt areas (−42%/−28%). Cork oak remained stable (1–4% growth). Other oaks showed minimal changes. Bushes (scrubland) declined sharply post-2007 (−31% BA/−6% NB). The most significant transformation occurred between 1995 and 2007, particularly in the south of Portugal, where wildfires promoted the replacement of maritime pine with eucalyptus, a species that offers greater profitability, leading to agricultural abandonment in burned areas. Full article
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17 pages, 3126 KiB  
Article
Investigating the Sensitivity of Modelled Ozone Levels in the Mediterranean to Dry Deposition Parameters
by André Barreirinha, Sabine Banzhaf, Markus Thürkow, Carla Gama, Michael Russo, Enrico Dammers, Martijn Schaap and Alexandra Monteiro
Atmosphere 2025, 16(5), 620; https://doi.org/10.3390/atmos16050620 - 19 May 2025
Viewed by 439
Abstract
The exposure to elevated levels of ozone contributes to respiratory diseases and ecosystem degradation. Mediterranean countries are among those most affected by high ozone concentrations, which are generally overestimated by chemistry transport models underscoring the importance of improving the accuracy of air quality [...] Read more.
The exposure to elevated levels of ozone contributes to respiratory diseases and ecosystem degradation. Mediterranean countries are among those most affected by high ozone concentrations, which are generally overestimated by chemistry transport models underscoring the importance of improving the accuracy of air quality modelling. This study introduces an enhanced Mediterranean dry deposition description within the LOTOS-EUROS model framework, focusing on refining key vegetation parameters for the Mediterranean climate zone, with the goal to better estimate deposition and connected concentration values. Adjustments were made to the vegetation type dependent Jarvis functions for temperature and vapour pressure deficit, as well as to the maximum stomatal conductance across four land use types: arable land, crops, deciduous broadleaf forest, and coniferous evergreen forest. The model’s baseline run showed a widespread overestimation of ozone. Adjustments to the dry deposition routines reduced this overestimation, but the model simulation incorporating all changes still showed elevated ozone levels. Both runs displayed moderate spatial correlation with observations from 117 rural background monitoring stations, and most stations exhibited a temporal correlation between 0.5 and 0.8. An improved RMSE and bias were noted at the majority of the stations (114 out of 117) for the model simulation incorporating all changes. The monthly analysis indicated consistent overestimation at two Portuguese sites beginning in March. The model effectively tracked temporal changes overall. However, the diurnal analysis revealed site-specific differences: an overestimation at the station closest to highly populated areas at night, while rural stations aligned better with observed values. These results highlight the benefits of region-specific model adaptations and lay the groundwork for further advancements, such as incorporating detailed vegetation classifications and seasonal variations. Full article
(This article belongs to the Section Atmospheric Techniques, Instruments, and Modeling)
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32 pages, 1924 KiB  
Review
A Comprehensive Comparison of Insulation Materials for Timber Building Systems
by Bernardino M. Rocha, Marina Tenório, Jorge M. Branco and Sandra M. Silva
Energies 2025, 18(10), 2420; https://doi.org/10.3390/en18102420 - 8 May 2025
Viewed by 1341
Abstract
The key objectives of both European Union and Portuguese policies are energy efficiency and carbon neutrality in the building sector. Timber construction offers unique advantages in achieving these goals, such as increased productivity through faster and more efficient building processes, using renewable resources [...] Read more.
The key objectives of both European Union and Portuguese policies are energy efficiency and carbon neutrality in the building sector. Timber construction offers unique advantages in achieving these goals, such as increased productivity through faster and more efficient building processes, using renewable resources with lower carbon emissions during production and throughout the lifecycle, and contributions to forest conservation. However, in many countries, timber construction remains underutilised due to concerns about its thermal and acoustic performance, fire safety, and limited availability of raw materials. This study addresses these challenges by evaluating the potential of various insulation materials, including polystyrenes, mineral wools, natural fibres, composites, and acoustic mats, for incorporation into prefabricated timber components. Key performance criteria included thermal insulation, sound absorption, fire reaction, environmental impact, and local availability. Among the materials analysed, glass wool, rock wool, and cork emerged as the most favourable options, offering excellent thermal and acoustic performance and presenting strong results in other key parameters. These findings underscore the potential of incorporating these materials into timber construction systems, contributing to developing sustainable and high-performance building solutions. Full article
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20 pages, 9095 KiB  
Article
Applying a Fire Exposure Metric in the Artificial Territories of Portugal: Mafra Municipality Case Study
by Sidra Ijaz Khan, Jennifer L. Beverly, Maria Conceição Colaço, Francisco Castro Rego and Ana Catarina Sequeira
Fire 2025, 8(5), 179; https://doi.org/10.3390/fire8050179 - 30 Apr 2025
Cited by 1 | Viewed by 1351
Abstract
Portugal’s increasing wildfire frequency has led to home destruction, large areas burned, ecological damage, and economic loss, emphasizing the need for effective fire exposure assessments. This study builds on a Canadian approach to wildfire exposure and evaluates wildfire exposure in the Portuguese municipality [...] Read more.
Portugal’s increasing wildfire frequency has led to home destruction, large areas burned, ecological damage, and economic loss, emphasizing the need for effective fire exposure assessments. This study builds on a Canadian approach to wildfire exposure and evaluates wildfire exposure in the Portuguese municipality of Mafra, using artificial territories (AT) as a proxy for the wildland–urban interface (WUI) and integrates land use land cover (LULC) data with a neighborhood analysis to map exposure at the municipal scale. Fire exposure was assessed for three fire transmission distances: radiant heat (RH, <30 m), short-range spotting (SRS, <100 m), and longer-range spotting (LRS, 100–500 m) using fine resolution (5 m) LULC data. Results revealed that while AT generally exhibited lower exposure (<16% “very high” exposure), adjacent hazardous LULC subtypes significantly increase wildfire hazard, with up to 51% of LULC subtypes classified as “very high exposure”. Field validation confirmed the accuracy of exposure maps, supporting their use in wildfire risk reduction strategies. This cost-effective, scalable approach offers actionable insights for forest and land managers, civil protection agencies, and policymakers, aiding in fuel management prioritization, community preparedness, and the design of evacuation planning. The methodology is adaptable to other fire-prone regions, particularly mediterranean landscapes. Full article
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17 pages, 2908 KiB  
Article
Valorisation of Forest Waste into Natural Textile Dyes—Case Study of Pine Cones
by Anna Barreto, Jorge M. Martins, Nuno Ferreira, Isabel Brás and Luisa H. Carvalho
Forests 2025, 16(5), 769; https://doi.org/10.3390/f16050769 - 30 Apr 2025
Viewed by 510
Abstract
The pine cone is an important forest product for the Portuguese economy. However, it is associated with environmental impacts, such as the generation of waste and the increased risk of forest fires. The objective of this research is to valorise waste from the [...] Read more.
The pine cone is an important forest product for the Portuguese economy. However, it is associated with environmental impacts, such as the generation of waste and the increased risk of forest fires. The objective of this research is to valorise waste from the production of Pinus pinaster Aiton in the form of natural dyes. The pine cone extracts were characterised in different alkaline solutions (1%, 5% and 10% NaOH) in order to evaluate the dyeing process on cotton knitwear, using the CIELab coordinates. The dyed samples were also subjected to light and water fastness tests. The extracts showed an increase in solids content with increasing alkalinity and a reduction in antioxidant content. The phenol content increased in the extract with 5% but decreased with the 10% concentration. All the dyes expressed a pink colour but with different shades. About the L* coordinate (luminosity), the colours became lighter as the NaOH increased. In the a* coordinate, all the samples had a reddish colour, and, in the b* coordinate, all the samples had a yellowish colour. About light and water fastness, all the samples lost colour, but in the water test, it was not noticeable. Full article
(This article belongs to the Section Wood Science and Forest Products)
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28 pages, 18628 KiB  
Article
Coupled Atmosphere–Fire Modelling of Pyroconvective Activity in Portugal
by Ricardo Vaz, Rui Silva, Susana Cardoso Pereira, Ana Cristina Carvalho, David Carvalho and Alfredo Rocha
Fire 2025, 8(4), 153; https://doi.org/10.3390/fire8040153 - 10 Apr 2025
Viewed by 585
Abstract
This study investigates the physical interactions and between forest fires and the atmosphere, which often lead to conditions favourable to instability and the formation of pyrocumulus (PyCu). Using the coupled atmosphere–fire spread modelling framework, WRF-SFIRE, the Portuguese October 2017 Quiaios wildfire, in association [...] Read more.
This study investigates the physical interactions and between forest fires and the atmosphere, which often lead to conditions favourable to instability and the formation of pyrocumulus (PyCu). Using the coupled atmosphere–fire spread modelling framework, WRF-SFIRE, the Portuguese October 2017 Quiaios wildfire, in association with tropical cyclone Ophelia, was simulated. Fire spread was imposed via burnt area data, and the fire’s influence on the vertical and surface atmosphere was analysed. Simulated local atmospheric conditions were influenced by warm and dry air advection near the surface, and moist air in mid to high levels, displaying an inverted “V” profile in thermodynamic diagrams. These conditions created a near-neutrally unstable atmospheric layer in the first 3000 m, associated with a low-level jet above 1000 m. Results showed that vertical wind shear tilted the plume, resulting in an intermittent, high-based, shallow pyroconvection, in a zero convective available potential energy environment (CAPE). Lifted parcels from the fire lost their buoyancy shortly after condensation, and the presence of PyCu was governed by the energy output from the fire and its updrafts. Clouds formed above the lifted condensation level (LCL) as moisture fluxes from the surface and released from combustion were lifted along the fire plume. Clouds were primarily composed of liquid water (1 g/kg) with smaller traces of ice, graupel, and snow (up to 0.15 g/kg). The representation of pyroconvective dynamics via coupled models is the cornerstone of understanding the phenomena and field applications as the computation capability increases and provides firefighters with real time extreme fire conditions or predicting ahead of time. Full article
(This article belongs to the Special Issue Fire Numerical Simulation, Second Volume)
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21 pages, 578 KiB  
Article
Burnout Risk Profiles in Psychology Students: An Exploratory Study with Machine Learning
by M. Graça Pereira, Martim Santos, Renata Magalhães, Cláudia Rodrigues, Odete Araújo and Dalila Durães
Behav. Sci. 2025, 15(4), 505; https://doi.org/10.3390/bs15040505 - 9 Apr 2025
Viewed by 1486
Abstract
University students are at increased risk of developing burnout and psychological distress from high academic workloads and performance expectations. The purpose of this study is to analyze the relationship between psychological and lifestyle variables and academic burnout, as well as to identify burnout [...] Read more.
University students are at increased risk of developing burnout and psychological distress from high academic workloads and performance expectations. The purpose of this study is to analyze the relationship between psychological and lifestyle variables and academic burnout, as well as to identify burnout risk profiles in psychology students. This study used a cross-sectional design and included 274 Portuguese psychology students, the majority being undergraduates (72.6%). Participants were assessed on psychological well-being, psychological distress, difficulties in emotional regulation, type of diet, physical activity, sleep quality, and burnout. The results showed that psychological distress, difficulties in emotional regulation, and sleep quality were positively associated with burnout, while psychological well-being was negatively associated. Using machine learning algorithms, two distinct profiles were found: “Burnout Risk” and “No Risk”. A total of 62 participants were identified as belonging to the burnout risk profile, showing higher levels of distress, emotional regulation difficulties, poor psychological well-being and sleep quality, pro-inflammatory diet, and less physical activity. The accuracy of the three machine learning models—Random Forest, XGBoost, and Support Vector Machine—was 95.06%, 93.82%, and 97.53%, respectively. These results suggest the importance of health promotion within university settings, together with mental health strategies focused on adaptive psychological functioning, to prevent the risk of burnout. Full article
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22 pages, 4712 KiB  
Article
Assessing Forestry Needs and Challenges in Portugal: Insights from the Sector Interested Parties
by Sofia Corticeiro, Helena Vieira, Mariana Almeida, Dionísia Laranjeiro, Ana Lillebø and Bruna R. F. Oliveira
Forests 2025, 16(3), 501; https://doi.org/10.3390/f16030501 - 12 Mar 2025
Viewed by 640
Abstract
Forests are one of the most predominant types of land usage in Portugal and are highly relevant in terms of environmental, economic, social, and political factors. Increasing the value and the resilience of the Portuguese forest, defining adequate policies, and aligning forest research [...] Read more.
Forests are one of the most predominant types of land usage in Portugal and are highly relevant in terms of environmental, economic, social, and political factors. Increasing the value and the resilience of the Portuguese forest, defining adequate policies, and aligning forest research with society needs requires a truthful comprehension of the most relevant challenges in this sector. This study identifies and analyzes the most relevant needs and challenges impacting the Portuguese forestry sector, both currently and over a five-year period, from the stakeholder’s perspective. A participatory approach was employed, engaging national and regional forest stakeholders, to ensure a realistic vision of the forest sector in Portugal. A total of 116 topics were identified, with a predominance of immediate challenges over future information needs, underscoring the urgent pressures on the sector. Environmental/ecological and policy issues dominated the identified needs and challenges, reflecting the urgency for strategic interventions in these areas. A significant emphasis was placed on the mitigation of climate change impacts, mainly associated with biotic and abiotic risks, promoting technological advanced forest management, and the sector valorization. Policy and legal issues, such as fragmented ownership and adequate economic and fiscal incentives, were also identified as major concerns. The findings highlight the interconnected nature of forestry challenges and the need for integrated, multidisciplinary, and transdisciplinary approaches, prioritizing research on climate impacts, developing adaptive management strategies, promoting stakeholder engagement, and enhancing capacity-building initiatives. The results of this study make it a relevant case study for other forest stakeholders in similar regions in Europe with comparative forest management models and can inspire new solutions for common challenges opening new research avenues for other forest related academics. Full article
(This article belongs to the Section Forest Economics, Policy, and Social Science)
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20 pages, 6234 KiB  
Review
Exploring the Multifaceted Aspects of Strawberry Tree (Arbutus unedo L.) Forests in Portugal
by Maria Nazaré Coelho Pinheiro, Filomena Gomes, Goreti Botelho, Ivo Rodrigues, Ruslan Mariychuk and Lyudmyla Symochko
Land 2025, 14(3), 468; https://doi.org/10.3390/land14030468 - 24 Feb 2025
Viewed by 1033
Abstract
This article explores the ecological role of strawberry tree forests (Arbutus unedo L.) in the resilience of Portuguese forest ecosystems and their relationship with plant production as a source of food. It discusses the importance of the strawberry tree in fire combat [...] Read more.
This article explores the ecological role of strawberry tree forests (Arbutus unedo L.) in the resilience of Portuguese forest ecosystems and their relationship with plant production as a source of food. It discusses the importance of the strawberry tree in fire combat and the improvement of agroforestry areas by mitigating erosion and augmenting soil organic matter. The multifunctionality of their fruits, emphasizing their utilization in food and beverage production, is also addressed. Moreover, the socio-economic and cultural significance of fruit production, emphasizing its role in sustainable development, is analyzed. The diversity of beverages (spirits, liquors, and gin) and food products (jams, jellies, etc.) effectively contribute to have a positive social and economic impact on the local populations and tradition maintenance. Moreover, the bioactive compounds in different parts of the plants and fruits have applications in pharmacology and cosmetics. Finally, the valorization of strawberry tree spirits mush waste as a source of natural dyes for textiles is discussed as a promising research topic to be explored in the future. Full article
(This article belongs to the Special Issue Agroforestry Systems for Biodiversity and Landscape Conservation)
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23 pages, 10921 KiB  
Article
A Weakly Supervised and Self-Supervised Learning Approach for Semantic Segmentation of Land Cover in Satellite Images with National Forest Inventory Data
by Daniel Moraes, Manuel L. Campagnolo and Mário Caetano
Remote Sens. 2025, 17(4), 711; https://doi.org/10.3390/rs17040711 - 19 Feb 2025
Cited by 1 | Viewed by 1219
Abstract
National Forest Inventories (NFIs) provide valuable land cover (LC) information but often lack spatial continuity and an adequate update frequency. Satellite-based remote sensing offers a viable alternative, employing machine learning to extract thematic data. State-of-the-art methods such as convolutional neural networks rely on [...] Read more.
National Forest Inventories (NFIs) provide valuable land cover (LC) information but often lack spatial continuity and an adequate update frequency. Satellite-based remote sensing offers a viable alternative, employing machine learning to extract thematic data. State-of-the-art methods such as convolutional neural networks rely on fully pixel-level annotated images, which are difficult to obtain. Although reference LC datasets have been widely used to derive annotations, NFIs consist of point-based data, providing only sparse annotations. Weakly supervised and self-supervised learning approaches help address this issue by reducing dependence on fully annotated images and leveraging unlabeled data. However, their potential for large-scale LC mapping needs further investigation. This study explored the use of NFI data with deep learning and weakly supervised and self-supervised methods. Using Sentinel-2 images and the Portuguese NFI, which covers other LC types beyond forest, as sparse labels, we performed weakly supervised semantic segmentation with a convolutional neural network to create an updated and spatially continuous national LC map. Additionally, we investigated the potential of self-supervised learning by pretraining a masked autoencoder on 65,000 Sentinel-2 image chips and then fine-tuning the model with NFI-derived sparse labels. The weakly supervised baseline achieved a validation accuracy of 69.60%, surpassing Random Forest (67.90%). The self-supervised model achieved 71.29%, performing on par with the baseline using half the training data. The results demonstrated that integrating both learning approaches enabled successful countrywide LC mapping with limited training data. Full article
(This article belongs to the Section Earth Observation Data)
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20 pages, 7537 KiB  
Article
Diversity and Distribution of Phytophthora Species Along an Elevation Gradient in Natural and Semi-Natural Forest Ecosystems in Portugal
by Carlo Bregant, Eduardo Batista, Sandra Hilário, Benedetto Teodoro Linaldeddu and Artur Alves
Pathogens 2025, 14(1), 103; https://doi.org/10.3390/pathogens14010103 - 20 Jan 2025
Cited by 1 | Viewed by 1280
Abstract
Globally, forests are constantly threatened by a plethora of disturbances of natural and anthropogenic origin, such as climate change, forest fires, urbanization, and pollution. Besides the most common stressors, during the last few years, Portuguese forests have been impacted by severe decline phenomena [...] Read more.
Globally, forests are constantly threatened by a plethora of disturbances of natural and anthropogenic origin, such as climate change, forest fires, urbanization, and pollution. Besides the most common stressors, during the last few years, Portuguese forests have been impacted by severe decline phenomena caused by invasive pathogens, many of which belong to the genus Phytophthora. The genus Phytophthora includes a large number of species that are invading forest ecosystems worldwide, chiefly as a consequence of global trade and human activities. This paper reports the results of a survey of Phytophthora diversity in natural and semi-natural forest ecosystems in Portugal along an elevation gradient. Isolations performed from 138 symptomatic plant tissues and rhizosphere samples collected from 26 plant species yielded a total of 19 Phytophthora species belonging to 6 phylogenetic clades, including P. cinnamomi (36 isolates), P. multivora (20), P. plurivora (9), P. cactorum (8), P. lacustris (8), P. pseudocryptogea (8), P. amnicola (6), P. hedraiandra (6), P. pseudosyringae (5), P. thermophila (5), P. bilorbang (4), P. inundata (4), P. asparagi (3), P. citricola (3), P. gonapodyides (3), P. rosacearum (3), P. chlamydospora (2), P. pachypleura (2), and P. syringae (1). Overall, the data obtained highlight the widespread occurrence of P. cinnamomi in natural ecosystems from sea level to mountain habitats. The results of the pathogenicity tests carried out on 2-year-old chestnut plants confirmed the key role of P. cinnamomi in the recrudescence of chestnut ink disease and the additional risk posed by P. pachypleura, P. plurivora, and P. multivora to Portuguese chestnut forests. Finally, three species, P. citricola, P. hedraiandra, and P. pachypleura, are reported for the first time in the natural ecosystems of Portugal. Full article
(This article belongs to the Special Issue Microbial Pathogenesis and Emerging Infections)
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15 pages, 3362 KiB  
Article
Assessing Atlantic Kelp Forest Restoration Efforts in Southern Europe
by Alexandre F. S. Marques, Álvaro Sanchéz-Gallego, Rodrigo R. Correia, Isabel Sousa-Pinto, Silvia Chemello, Inês Louro, Marco F. L. Lemos and João N. Franco
Sustainability 2024, 16(21), 9176; https://doi.org/10.3390/su16219176 - 23 Oct 2024
Cited by 2 | Viewed by 2092
Abstract
Kelp forests are essential marine ecosystems increasingly compromised by human activities. Effective reforestation strategies are urgently needed, and the “green gravel” method is a viable tool already used in some European regions. This study aimed to assess the success of this method using [...] Read more.
Kelp forests are essential marine ecosystems increasingly compromised by human activities. Effective reforestation strategies are urgently needed, and the “green gravel” method is a viable tool already used in some European regions. This study aimed to assess the success of this method using the native Kelp species Laminaria ochroleuca on the Portuguese coastline. Cultures of green gravel were reared until the specimens reached a size of approximately 3 cm. The gravel was then deployed at selected sites in Peniche, Berlengas, and Cascais. Over an eight-month period, scientific scuba divers monitored the integration of Kelp, along with associated fish, invertebrate, and algae communities. Nutrient availability, temperature, water movement, substrate type, and Rugosity Index (RI) were also measured. The highest success rate was 12% in Consolação, with Elefante and Galos (Berlengas) reaching 7% and 4%, respectively. By the end of the monitoring period, Cascais had no remaining Kelp on green gravel. Present data suggest that higher success is dependent on less rugged and higher RI topography. Higher grazing pressure, rougher terrain, and unexpected sedimentation appear to be the main obstacles to deployment success. Solid knowledge (biologic and topographic) on the restoration site, starting restoration actions near already established Kelp forests, and significantly scaling up restoration efforts could substantially improve the success of the green gravel method in future reforestation campaigns. Full article
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13 pages, 1880 KiB  
Article
Screening Biogenic Volatile Organic Compounds from Common Portuguese Shrubs Using Headspace–Bar Adsorptive Microextraction (HS-BAµE)
by Jéssica S. R. F. Cerqueira and José M. F. Nogueira
Separations 2024, 11(9), 264; https://doi.org/10.3390/separations11090264 - 9 Sep 2024
Viewed by 1298
Abstract
In this study, headspace–bar adsorptive microextraction (HS-BAµE) combined with gas chromatography–mass spectrometry (GC-MS) was employed to screen the major biogenic volatile organic compounds (BVOCs) emitted by six different Portuguese shrub species (Erica scoparia L., Cistus ladanifer L., Cistus monspeliensis L., Lavandula stoechas [...] Read more.
In this study, headspace–bar adsorptive microextraction (HS-BAµE) combined with gas chromatography–mass spectrometry (GC-MS) was employed to screen the major biogenic volatile organic compounds (BVOCs) emitted by six different Portuguese shrub species (Erica scoparia L., Cistus ladanifer L., Cistus monspeliensis L., Lavandula stoechas L., Thymus villosus L., and Thymus camphoratus). The HS-BAµE/GC-MS methodology was developed, optimized, and validated using five common monoterpenoids (α-pinene, β-pinene, limonene, 1,8-cineole, and thymol) and one sesquiterpenoid (caryophyllene oxide). Under optimized experimental conditions (microextraction-sorbent phase: activated carbon (CN1), 3 h (35 °C); back-extraction: n-C6 (1 h)), good efficiencies (>45%), low analytical thresholds (5.0–15.0 µg/L) and suitable linear dynamic ranges (20.0–120.0 µg/L, r2 > 0.9872) were achieved, as well as acceptable intra and inter-day precisions (RSD ≤ 30.1%). Benchmarking the proposed methodology, HS-BAµE(CN1), against the reference methodology, HS-SPME(PDMS/DVB), revealed comparable analytical responses and demonstrated excellent reproducibility. Among the six shrub species studied, Thymus camphoratus exhibited the highest emissions of BVOCs from its leaves, notably, 1,8-cineole (4136.9 ± 6.3 µg/g), α-pinene (763.9 ± 0.5 µg/g), and β-pinene (259.3 ± 0.5 µg/g). It was also the only species found to release caryophyllene oxide (411.4 ± 0.3 µg/g). The observed levels suggest that these shrub species could potentially serve as fuel sources in the event of forest fires occurring under extreme conditions. In summary, the proposed methodology proved to be a favorable analytical alternative for screening BVOCs in plants. It not only exhibited remarkable performance but also demonstrated user- and eco-friendliness, cost-effectiveness, and ease of implementation. Full article
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