Search Results (16,320)

Due to their low permeability, the location of natural fractures is key to the successful development of buried hill reservoirs. Due to the high degree of rock fragmentation and strong absorption of seismic waves at the top of buried hill formations, it is hard to identify the distribution of natural fractures inside a buried hill using conventional seismic methods. To overcome this difficulty, this study proposes a natural fracture identification technology for buried hill reservoirs that combines a continuum damage mechanics model with finite element numerical simulation. A 3D numerical solution workflow is established to determine the natural fracture distribution in target buried hill reservoirs. By constructing a geological model of a block, reconstructing the orogenic history, developing a 3D finite element model, and performing numerical simulations, the multi-stage orogenic processes experienced by buried hill reservoirs and the resultant natural fracture formation are replicated. This approach yields 3D numerical results of natural fracture distribution. Using the G-Block in the Zhongyuan Oilfield as a case study, the natural fracture distribution in a buried hill reservoir composed of mixed lithologies, including marble and Carboniferous formations, within the faulted G6-well group is analyzed. The results include plane views of the contour of damage variable SDEG, which represents the fracture distribution within the subsurface layer at 600 m intervals below the buried hill surface, as well as a vertical sectional view of the contour of SDEG’s distribution along specified well trajectories. By comparison with the results of the fracture distribution obtained with logging data, a consistency of 87.5% is achieved. This indicates the reliability of the numerical results for natural fractures obtained using the technology presented here. Full article

The gas reservoir of the Sinian Dengying Formation (Member 4) in Sichuan Basin exhibits extensive development of inter-clast dissolution pores and vugs within its carbonate reservoirs, characterized by low porosity (average 3.21%) and low permeability (average 2.19 mD). With the progressive development of the Moxi (MX)structure, the existing stimulation techniques require further optimization based on the specific geological characteristics of these reservoirs. Through large-scale true tri-axial physical simulation experiments, this study systematically evaluated the performance of three principal acid systems in reservoir stimulation: (1) Self-generating acid systems, which enhance etching through the thermal decomposition of ester precursors to provide sustained reactive capabilities. (2) Gelled acid systems, characterized by high viscosity and effectiveness in reducing breakdown pressure (18%~35% lower than conventional systems), are ideal for generating complex fracture networks. (3) Diverting acid systems, designed to improve fracture branching density by managing fluid flow heterogeneity. This study emphasizes hybrid acid combinations, particularly self-generating acid prepad coupled with gelled acid systems, to leverage their synergistic advantages. Field trials implementing these optimized systems revealed that conventional guar-based fracturing fluids demonstrated 40% higher breakdown pressures compared to acid systems, rendering hydraulic fracturing unsuitable for MX reservoirs. Comparative analysis confirmed gelled acid’s superiority over diverting acid in tensile strength reduction and fracture network complexity. Field implementations using reservoir-quality-adaptive strategies—gelled acid fracturing for main reservoir sections and integrated self-generating acid prepad + gelled acid systems for marginal zones—demonstrated the technical superiority of the hybrid system under MX reservoir conditions. This optimized protocol enhanced fracture length by 28% and stimulated reservoir volume by 36%, achieving a 36% single-well production increase. The technical framework provides an engineered solution for productivity enhancement in deep carbonate gas reservoirs within the G-M structural domain, with particular efficacy for reservoirs featuring dual low-porosity and low-permeability characteristics. Full article

Blood–brain barrier (BBB) deterioration with increasing age is an important factor contributing to vascular dementia. Previous studies show that endothelial nitric oxide synthase (eNOS) facilitates vascular endothelial growth factor-mediated angiogenesis and increased vascular permeability. In contrast, recent work has shown that aged hemi-deficient hemizygous eNOS^+/− mice manifest BBB disruption in association with increased incidence of thromboembolic events in the brain. To unravel whether eNOS contributes to or protects against hypoxia-induced cerebrovascular damage, we compared chronic mild hypoxia (CMH)-induced cerebrovascular angiogenic remodeling and BBB breakdown in aged (20 months old) eNOS^+/− and wild-type (WT) mice. This revealed that CMH strongly enhanced eNOS expression in cerebral blood vessels with much lower levels in eNOS^+/− mice. eNOS hemi-deficiency resulted in greater CMH-induced BBB disruption, but unexpectedly, had no effect on endothelial proliferation. eNOS^+/− mice also displayed enhanced endothelial expression of the endothelial activation markers MECA-32, VCAM-1, and β3 integrin in cerebral blood vessels, indicating greater vascular inflammation, and this correlated with increased levels of microglial activation and demyelination. Taken together, our results support the concept that eNOS plays an important protective function in the aged brain by suppressing endothelial activation and maintaining cerebrovascular health. Full article

Ozone pollution is a significant public health problem due to its association with chronic diseases. This study examines the effects of repeated exposure to low doses of ozone on intestinal barrier function in rats. Seventy-two male Wistar rats were divided into six groups. The control group was exposed to normal air, while the ozone groups received a dose of 0.25 ppm for four hours daily for periods of 7, 15, 30, 60, and 90 days, respectively. After treatment, the duodenum, jejunum, and colon were removed and analyzed by biochemical assays, Western blot, immunohistochemistry, and histological techniques. The results indicated an increase in oxidized lipids and structural alterations in the duodenum and jejunum after 7 days of ozone exposure. The result showed changes in haptoglobin, IL-1β, and IL-6. In addition, increased immunoreactivity varied according to intestinal structure and the duration of ozone exposure in the duodenum, jejunum, and colon. In conclusion: Ozone exposure causes an increase in proinflammatory cytokines that leads to a loss of regulation of the immune response in the duodenum, jejunum, and colon of rats, as well as structural changes that alter the intestinal barrier and perpetuate a state of chronic inflammation characteristic of inflammatory bowel diseases. Full article

The lithological dichotomy in the Hammam Faraun Member (Gulf of Suez, Egypt) reveals a stable western flank with Nullipore carbonate deposits, contrasting with the clastic-prone eastern margin influenced by tectonic activity. This study aims to decipher multifactorial controls on spatial lithological variability and reservoir implications through (1) foraminiferal-based paleoenvironmental reconstruction; (2) integrated sequence stratigraphic–petrophysical analysis for sweet spot identification; and (3) synthesis of lateral facies controls. This study uniquely integrates foraminiferal paleoenvironmental proxies, sequence stratigraphy, and petrophysical analyses to understand the multifactorial controls on spatial variability and its implications for reservoir characterization. Middle Miocene sea surface temperatures, reconstructed between 19.2 and 21.2 °C, align with warm conditions favorable for carbonate production across the basin. Foraminiferal data indicate consistent bathyal depths (611–1238 m) in the eastern region, further inhibited in photic depths by clastic influx from the nearby Nubian Shield, increasing turbidity and limiting carbonate factory growth. Conversely, the western shelf, at depths of less than 100 m, supports thriving carbonate platforms. In the sequence stratigraphy analysis, we identify two primary sequences: LA.SQ1 (15.12–14.99 Ma), characterized by evaporitic Feiran Member deposits, and LA.SQ2 (14.99–14.78 Ma), dominated by clastic deposits. The primary reservoir comprises highstand systems tract (HST) sandstones with effective porosity ranging from 17% to 22% (calculated via shale-corrected neutron density cross-plots) and hydrocarbon saturation of 33%–55% (computed using Archie’s equation). These values, validated in Wells 112-58 (ϕe = 19%, S_hc = 55%) and 113M-81 (ϕe = 17%, S_hc = 33%), demonstrate the primary reservoir potential. Authigenic dolomite cement and clay content reduce permeability in argillaceous intervals, while quartz dissolution in clean sands enhances porosity. This research emphasizes that bathymetry, sediment availability, and syn-sedimentary tectonics, rather than climate, govern carbonate depletion in the eastern region, providing predictive parameters for identifying reservoir sweet spots in clastic-dominated rift basins. Full article

The dynamic process of water depletion plays a critical role in both surface coalbed methane (CBM) development and underground gas extraction, reshaping water–rock interactions and inducing complex permeability responses. Addressing the limited understanding of the coupling mechanism between heterogeneous pore water evolution and permeability during dynamic processes, this study simulates reservoir transitions across four zones (prospective planning, production preparation, active production, and mining-affected zones) via centrifugal experiments. The results reveal a pronounced scale dependence in pore water distribution. During low-pressure stages (0–0.54 MPa), rapid drainage from fractures and seepage pores leads to a ~12% reduction in total water content. In contrast, high-pressure stages (0.54–3.83 MPa) promote water retention in adsorption pores, with their relative contribution rising to 95.8%, forming a dual-structure of macropore drainage and micropore retention. Multifractal analysis indicates a dual-mode evolution of movable pore space. Under low centrifugal pressure, D₋₁₀ and Δα decrease by approximately 34% and 36%, respectively, reflecting improved connectivity within large-pore networks. At high centrifugal pressure, an ~8% increase in D₀−D₂ suggests that pore-scale heterogeneity in adsorption pores inhibits further seepage. A quantitative coupling model establishes a quadratic relationship between fractal parameters and permeability, illustrating that permeability enhancement results from the combined effects of pore volume expansion and structural homogenization. As water saturation decreases from 1.0 to 0.64, permeability increases by more than 3.5 times. These findings offer theoretical insights into optimizing seepage pathways and improving gas recovery efficiency in dynamically evolving reservoirs. Full article

The thermo-hydrological (TH) coupling model constitutes the foundational framework for investigating the temperature distribution of surrounding rock in cold region tunnels. In this study, a fully coupled TH model is proposed that takes into account multiple physical phenomena during the freezing process of surrounding rock. Firstly, the model was established based on thermodynamics, seepage theory, and ice–water phase change theory, which accounted for unfrozen water, latent heat of phase change, ice impedance, and convective heat transfer. The model was successfully verified by comparing its results to field data. Next, the sensitivity of surrounding rock temperature to environmental, thermodynamic, seepage, and coupling parameters in the fully coupled TH model was systematically studied using a numerical analysis method. The results show that the annual temperature amplitude and thermal conductivity represent the main factors affecting the surrounding rock temperature at a radial depth of 0 m, while the initial temperature and porosity are the key factors at a radial depth of 5 m. Permeability has the least influence on the surrounding rock temperature, but the temperature field will experience sudden changes if its value exceeds its value exceeds 1 × 10⁻¹² m². Finally, using the proposed numerical model, the thickness of insulation layer was simulated, and the degree of influence of the parameters on the thickness of insulation layer was analyzed. This study reveals that the annual temperature amplitude has the greatest influence on the calculation of insulation layer thickness, with its normalized sensitivity factor being approximately 50%. These findings not only expand the methodology for exploring the laws of TH coupling but also provide a theoretical foundation for improving the parameter calibration efficiency and calculation accuracy of the fully coupled TH model, and they have significant reference value. Full article

This study introduces an innovative strategy for active, biodegradable food packaging through the incorporation of gamma-decalactone (GDL), a natural aromatic compound with antimicrobial properties, into apple-pectin-based edible films. The addition of GDL significantly modified the film structure, resulting in enhanced light barrier properties (the opacity increased from 1.10 to 8.64 a.u./mm), a more porous microstructure (confirmed by SEM), and reduced tensile strength (from 13.84 to 5.68 MPa). The films also exhibited lower water vapour sorption (from 1.45 to 0.80 g/g dry matter (d.m.) and increased gas permeability. FTIR analysis confirmed interactions between GDL and the polymer matrix. The films with GDL added exhibited antimicrobial properties against various microbial species, such as bacteria, yeasts, and moulds. A 5% addition of GDL to the coating completely inhibited the growth of Bacillus subtilis bacteria and Yarrowia lipolytica, reducing the number of yeast cells by 3 log units (after 48 h of culture, from 7.11 ± 0.09 to 4.09 ± 0.27 log CFU/mL) and limiting Monilinia fructicola mycelium growth by 70%. These results highlight GDL’s dual function as a natural aromatic and antimicrobial agent, supporting its potential application in sustainable packaging for perishable foods. Full article

Electrochemical sensors have been developed based on polyethylene terephthalate track-etched membranes (PET TeMs) modified by photograft copolymerization of N-vinylformamide (N-VFA) and trimethylolpropane trimethacrylate (TMPTMA). The modification, structure and properties of the modified PET TeMs were thoroughly characterized using scanning electron microscopy (SEM) and atomic force microscopy (AFM), thermogravimetric analysis (TGA), Fourier-transform infrared (FTIR) spectroscopy, gas permeability measurements and contact angle analysis. Optimal membrane modification was achieved using C = 10% (N-VFA), 60 min of UV irradiation and a UV lamp distance of 10 cm. Furthermore, the modified membranes were implemented in a two-electrode configuration for the determination of Eu³⁺, Gd³⁺, La³⁺ and Ce³⁺ ions via square-wave anodic stripping voltammetry (SW-ASV). The sensors exhibited a linear detection range from 10⁻⁷ M to 10⁻³ M, with limits of detection of 1.0 × 10⁻⁶ M (Eu³⁺), 6.0 × 10⁻⁶ M (Gd³⁺), 2.0 × 10⁻⁴ M (La³⁺) and 2.5 × 10⁻⁵ M (Ce³⁺). The results demonstrated a significant enhancement in electrochemical response due to the grafted PET TeMs-g-N-PVFA-TMPTMA structure, and the sensor showed practical applicability and consistent performance in detecting rare earth ions in tap water. Full article

Background/Objectives: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is an urgent public health threat due to its rapid dissemination and resistance to last-line antibiotics. Cefiderocol (FDC), a novel siderophore cephalosporin, targets resistant Gram-negative pathogens by exploiting bacterial iron uptake mechanisms. However, resistance to FDC is emerging among Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae strains. This study characterizes a spontaneous FDC-resistant subpopulation (IHC216) derived from a KPC-producing strain (KPNMA216) using comprehensive genomic, transcriptional, and phenotypic analyses. Methods: Given the whole-genome sequencing results, where mutations were identified in genes involved in transcriptional regulation and membrane permeability (ompC) among others, in the present work we further explore their potential implications and conduct a more detailed analysis of the IHC216 genome. A qRT-PCR analysis highlighted significant downregulation of classical siderophore-mediated iron acquisition systems (fepA, cirA, iroN) and upregulation of alternative iron uptake pathways (iucA, fiU), reflecting a switch in iron acquisition strategies. Results: A notable downregulation of bla_KPC-163 correlated with restored susceptibility to carbapenems, indicating collateral susceptibility. Altered expressions of pbp2 and pbp3 implicated adaptive changes in cell wall synthesis, potentially affecting FDC resistance mechanisms. Furthermore, enhanced oxidative stress responses via upregulated sodC expression and increased capsule production were observed. Conclusions: These findings underscore the complex interplay of genetic and transcriptional adaptations underlying FDC resistance, highlighting potential therapeutic vulnerabilities. Full article

Background/Objectives: Normal-weight obesity describes those with a normal body mass index (BMI) and high body fat percent. Older adults with normal-weight obesity (NWO-O) are at increased risk for cardiovascular disease (CVD), but underlying mechanisms remain unclear. This pilot study examined whether NWO-O had an unfavorable cardiometabolic response to acute high-fat meal intake compared to normal BMI, low body fat percent individuals that were both older (NWL-O) and younger (NWL-Y). Methods: Participants (N = 29) with a normal BMI were grouped as follows: NWL-Y (18–35 years, low body fat percent; n = 12), NWL-O (≥60 years, low body fat percent; n = 9), and NWO-O (≥60 years, high body fat percent; n = 8). All participants completed an abbreviated fat tolerance test (75 g fat). Fasting and 4 h blood samples were collected to measure lipids (triglycerides and high-density lipoprotein cholesterol [HDL-C]), biomarkers of intestinal permeability (lipopolysaccharide binding protein [LBP] and soluble cluster of differentiation [sCD14]), and the inflammatory marker interleukin (IL)-6. Results: NWO-O had higher percent, absolute, and trunk fat compared to NWL-Y and NWL-O (p’s ≤ 0.01). Conversely, percent lean mass was lower in NWO-O versus both NWL groups (p’s ≤ 0.01). NWO-O had higher fasting triglycerides than NWL-Y (p < 0.05), but all groups were in the clinically normal range on average (≤107 mg/dL). However, NWO-O had higher 4 h triglycerides (239.4 ± 101.0 mg/dL) compared to NWL-Y and NWL-O (p < 0.01), consistent with an adverse response. The absolute change in triglycerides was higher in NWO-O relative to NWL-Y (p < 0.01), but not compared to NWL-O (p = 0.06). Fasting IL-6 was higher in NWO-O relative to NWL-Y (p < 0.05). Fasting and 4 h sCD14 were similarly higher in NWL-O and NWO-O versus NWL-Y (p’s < 0.01). Conclusions: NWO-O had an exaggerated postprandial triglyceride response compared to younger and similar-aged NWL individuals, which could reflect hepatic very low-density lipoprotein overproduction or impaired triglyceride clearance. Future work should continue to investigate the role of postprandial dyslipidemia in NWO-O’s reported CVD risk. Full article

The Jiaoshiba area, as an important production capacity contribution block for the Fuling shale gas field, is of great significance for its long-term stable production. This study is based on continuous coring, and uses methods such as whole-rock mineral analysis, porosity and permeability analysis, gas content analysis, and organic geochemistry to systematically analyze the influencing factors of reservoir properties and gas content in the studied interval. Combined with the variation law of TOC and other parameters with depth, the target reservoir is comprehensively evaluated, and the evaluation results are verified based on actual production data. The results show that the influence of minerals on permeability is very weak, and cracks can greatly improve permeability, but their contribution to porosity is not significant. Porosity has a certain impact on gas content, but it is not the main controlling factor. The pores related to quartz (organic silicon) are mostly organic pores, which host a large amount of shale gas, while clay minerals are not conducive to the occurrence of shale gas. Organic matter (OM) maturity contributes more to porosity than OM abundance, but OM abundance has a stronger impact on gas content than its maturity. The research intervals can be divided into four categories: Class I (①–③) is the best, followed by Class II (⑦–⑨); Class III (④–⑥) is poor, and Class IV (top, non-gas-bearing layer) is the worst. Full article

Background/Objectives: Gut microbial dysbiosis, leaky gut, and increased transepithelial translocation of commensal bacteria have been documented in multiple sclerosis (MS). Intrathecal IgGs specific for Akkermansia muciniphila, a gut bacterium, are increased in patients with MS and associated with clinical disability. Our objective here was to explore the putative involvement of intrathecal anti-A. muciniphila IgG in MS pathogenesis by characterizing patients with different anti-A. muciniphila IgG indices. Methods: Serum and intrathecal IgG specific for A. muciniphila and other gut bacteria, as well as routine cerebrospinal fluid (CSF) parameters, were measured in 61 patients with MS. Examination of these patients included immunophenotyping of CSF-infiltrating and paired circulating lymphocytes, intrathecal markers of neurodegeneration and inflammation, and a detailed characterization of demographic, clinical, and magnetic resonance imaging (MRI) features. Results: Plasma blasts (p < 0.01), B cells (p < 0.01), and Th2 cells (p < 0.01), which might be involved in antibody production, were increased in the CSF of these patients, as well as blood pro-inflammatory Th17 cells (p < 0.05). Anti-A. muciniphila IgG indices were negatively associated with blood-brain barrier (BBB) permeability and circulating monocytes (p < 0.001), and positively with brain lesion load (p < 0.01). Conclusions: The differences between patients with low and high anti-A. muciniphila IgG indexes regarding BBB permeability, CSF cell infiltrates, and pro-inflammatory peripheral immune cells, as well as imaging features, support a role of anti-A. muciniphila immune response in MS pathogenesis. Full article

To address the critical challenge of ensuring bottom water-inrush stability during the excavation of ultra-deep foundation pits for riverside suspension-bridge anchorages under complex geological conditions involving high-pressure confined groundwater, we investigate the application of D-RJP high-pressure rotary jet grouting pile technology for ground improvement. Its effectiveness is systematically validated through a case study of the South Anchorage Foundation Pit for the North Channel Bridge of the Zhangjinggao Yangtze River Bridge. The D-RJP method led to the successful construction of a composite foundation within the soft soil that satisfies the permeability coefficient, interface friction coefficient, bearing capacity, and shear strength requirements, significantly improving the geotechnical performance of the anchorage foundation. A series of field experiments were conducted to optimize the critical construction parameters, including the lifting speed, water–cement ratio, and stroke spacing. Core sampling and laboratory testing revealed the grout columns to have good structural integrity. The unconfined compressive strength of the high-pressure jet grout columns reached 5.45 MPa in silty clay layers and 8.21 MPa in silty sand layers. The average permeability coefficient ranged from 1.67 × 10⁻⁷ to 2.52 × 10⁻⁷ cm/s. The average density of the columns was 1.66 g/cm³ in the silty clay layer and 2.08 g/cm³ in the silty sand layer. The cement content in the return slurry varied between 18% and 27%, with no significant soil squeezing effect observed. The foundation interface friction coefficient ranged from 0.44 to 0.52. After excavation, the composite foundation formed by D-RJP columns was subjected to static load and direct shear testing. The results showed a characteristic bearing capacity value of 1200 kPa, the internal friction angle exceeded 24.23°, and the cohesion exceeded 180 kPa. This study successfully verifies the feasibility of applying D-RJP technology to construct high-performance artificial composite foundations in complex strata characterized by deep soft soils and high-pressure confined groundwater, providing valuable technical references and practical insights for similar ultra-deep foundation pit projects involving suspension bridge anchorages. Full article

This study focuses on the complex pore structure and pronounced heterogeneity of tight sandstone reservoirs in the Linxing area of the Ordos Basin and develops a multi-scale quantitative characterization approach to investigate the coupling mechanism between pore structure and reservoir properties. Six core samples were selected from the Shiqianfeng Formation (depth interval: 1326–1421 m) for detailed analysis. Cast thin sections and scanning electron microscopy (SEM) experiments were employed to characterize pore types and structural features. Nuclear magnetic resonance (NMR) experiments were conducted to obtain T₂ spectra, which were used to classify bound and movable pores, and their corresponding fractal dimensions were calculated separately. In addition, NMR logging data from the corresponding well intervals were integrated to assess the applicability and consistency of the fractal characteristics at the logging scale. The results reveal that the fractal dimension of bound pores shows a positive correlation with porosity, whereas that of movable pores is negatively correlated with permeability, indicating that different scales of pore structural complexity exert distinct influences on reservoir performance. Mineral composition affects the evolution of pore structures through mechanisms such as framework support, dissolution, and pore-filling, thereby further enhancing reservoir heterogeneity. The consistency between logging responses and experimental observations verifies the regional applicability of fractal analysis. Bound pores dominate within the studied interval, and the vertical variation of the PMF/BVI ratio aligns closely with both the NMR T₂ spectra and fractal results. This study demonstrates that fractal dimension is an effective descriptor of structural characteristics across different pore types and provides a theoretical foundation and methodological support for the evaluation of pore complexity and heterogeneity in tight sandstone reservoirs. Full article