Search Results (375,872)

This study investigates the influence of PLA flowability and talc content on the performance of compostable thermoplastic starch/poly(butylene succinate) (TPS/PBS)-based systems for rigid applications. Different PLA grades with varying melt flow index (PLA23, PLA8 and PLA70) and talc contents (0, 5 and 10 wt%) were incorporated. Twelve formulations were compounded by twin-screw extrusion and processed by injection moulding. FTIR confirmed the coexistence of TPS, PBS and PLA phases without evidence of chemical interactions. Morphological analysis showed that PLA flowability plays a key role in phase distribution, with higher-flow PLA promoting improved dispersion and interfacial adhesion, while talc addition (5 and 10 wt%) increased structural heterogeneity; at higher loadings, particularly, DSC analysis revealed that talc acted as a nucleating agent for the PBS phase, increasing crystallisation temperatures from approximately 73 °C to 81 °C depending on formulation. Mechanical results showed that Young’s modulus increased from approximately 1.4 GPa to 2.7 GPa with decreasing PLA flowability and increasing talc content. Formulations containing low-flow PLA reached tensile strengths close to 32 MPa, although elongation at break decreased to values near 2%. In contrast, high-flow PLA formulations exhibited a more balanced mechanical response, with elongation values up to approximately 8%, associated with improved phase dispersion. Hybrid PLA systems showed intermediate behaviour, reaching elongations up to 22% while maintaining modulus values around 1.8 GPa. Talc provided additional reinforcement but reduced deformation capacity. HDT values remained relatively constant, indicating limited improvement in thermomechanical resistance despite increased stiffness. These results demonstrate that the combined control of PLA molecular characteristics and talc content enables tuning of the mechanical and thermomechanical performance of TPS/PBS/PLA/talc systems for rigid packaging applications. Full article

Background/Objectives: White–Sutton syndrome (WHSUS; OMIM 616364) is a rare neurodevelopmental disorder caused by pathogenic variants in the POGZ gene and characterized by developmental delay, intellectual disability, speech impairment, autism spectrum features, and dysmorphic traits. Although most reported cases are sporadic, inherited forms are exceptionally rare. We describe a familial case of WHSUS involving an affected mother and two children carrying a heterozygous POGZ nonsense variant, highlighting marked intra-familial phenotypic variability and expanding the clinical spectrum of the disorder. Methods: Clinical evaluation included multidisciplinary assessments. Genetic testing was performed using clinical exome sequencing (CES) with a virtual neurodevelopmental disorder (NDD) gene panel, followed by Sanger confirmation and segregation analysis in family members. The POGZ transcript reference NM_015100.3 was used for variant nomenclature and verified with the Mutalyzer tool. CNV detection from NGS data was performed using the Alissa CNV caller (Agilent) and visualized via IGV; the Xp11.22 microduplication was confirmed by chromosomal microarray (aCGH) and parental segregation analyses. Results: CES identified the heterozygous pathogenic POGZ variant c.1522C>T (p.Arg508*) in the female proband (III₆), an infant presenting with global developmental delay, hypotonia, speech impairment, gait abnormalities, and characteristic dysmorphic features. Segregation analysis demonstrated maternal inheritance and confirmed the presence of the variant in her affected brother (III₄), who also carries a de novo 1.79 kb microduplication at Xp11.22, while the maternal grandparents tested negative, indicating a de novo origin in the mother. The mother exhibited an attenuated phenotype, including mild neuropsychiatric and gastrointestinal manifestations. The variant is predicted to undergo nonsense-mediated decay (NMD), consistent with a moderate clinical presentation; however, experimental validation was not performed. Conclusions: This report documents a rare familial occurrence of WHSUS with highly variable expressivity. Our findings broaden the phenotypic and molecular characterization of POGZ-related disorders and emphasize the importance of comprehensive segregation studies and early genomic diagnosis. While experimental data link POGZ deficiency to DNA repair defects, no longitudinal clinical studies have demonstrated increased cancer risk in WHSUS; therefore, formal malignancy screening guidelines cannot be established at present, and this issue deserves future study in larger cohorts or registries. Full article

It is well-known that activation functions are crucial in determining spectral expressiveness, training dynamics, and reconstruction accuracy in implicit neural representations (INRs), which employ coordinate-based multilayer perceptrons to represent continuous signals. Despite showing excellent performance, sinusoidal activations, for example SIREN, are limited in their adaptability to diverse signal types due to their fixed harmonic structure. In this paper, we propose two novel periodic activation functions for INRs. (1) Harmonic generalizes sinusoidal activations by combining the fundamental frequency with learned second and third harmonics through per-neuron trainable amplitude coefficients, resulting in a richer spectral basis within the SIREN initialization framework. (2) PM-FINER (Phase-Modulated FINER) extends the variable-periodic FINER activation by embedding frequency modulation synthesis directly into the instantaneous phase, enabling data-driven phase distortion via a learnable modulation index and carrier ratio. We conducted comprehensive experiments spanning nine architectural configurations (including SIREN, WIRE, FINER, Gaussian, Harmonic, PM-FINER, and an additional direct comparison against the Subtractive Modulative Network (SMN)), using six natural images, three learning rate schedulers, and three random seeds, totaling 486 main training runs (534 runs total including an ${\omega }_0$ sensitivity sweep). Our evaluation combined peak signal-to-noise ratio (PSNR), structural similarity index (SSIM), and rigorous statistical analysis, such as paired t-tests, Wilcoxon signed-rank tests, Cohen’s d effect sizes, and Friedman rank tests. Under cosine annealing, Harmonic achieves a mean PSNR gain of 6.08 dB over SIREN and 2.57 dB over FINER (both $p<0.001$, Cohen’s $d>3.7$), while PM-FINER ranks statistically on par with Harmonic (mean difference $0.17$ dB, $p=0.36$), outperforming all of the other baselines. Compared with SMN, Harmonic outperforms it by $+7.94$ dB under cosine annealing (Bonferroni-adjusted $p<{10}^{-5}$, Cohen’s $d=12.3$), winning on all six images. Additionally, the Friedman ranking across the six images confirmed Harmonic (with mean rank $=1.33$) and PM-FINER (with mean rank $=1.67$), being the top two methods under cosine annealing. Our results establish interpretable multi-harmonic and phase-modulated activations as real alternatives to the existing INR activation functions. Full article

Background/Objectives: Lipid nanoparticles (LNPs) have emerged as crucial vehicles for messenger RNA (mRNA) applications in antitumor therapy. Combining LNPs with stimulator of interferon genes (STING) activation holds promise for treating “cold” tumors such as pancreatic cancer. However, two major challenges remain: inefficient mRNA escape from endosomes and STING pathway suppression in immunosuppressive tumor microenvironments. Methods: To improve endosomal escape, we developed a novel pH-responsive PEGylated lipid (Ben-mPEG₂₀₀₀) for mRNA-LNP preparation while using commercial Man-mPEG₂₀₀₀ for dendritic cell (DC)-targeted delivery of LNPs; to alleviate suppression of the STING pathway in the tumor microenvironment and activate immune responses, STING-R283S mRNA was encapsulated into LNPs, ultimately resulting in DC-targeted/pH-responsive LNPs loaded with STING-R283S mRNA for pancreatic cancer immunotherapy research. Results: After pH-responsive cleavage, Ben-mPEG₂₀₀₀ not only enhanced the positive charge of LNPs through the exposed protonated amino groups but also eliminated the PEG-induced steric hindrance effect. The combination of these two effects promoted membrane fusion between LNPs and the endosome, thereby enhancing mRNA translation. As a payload, STING-R283S could further amplify STING signaling in DCs without cytotoxicity to counteract immunosuppression in pancreatic cancer. Conclusions: This engineered LNP platform enhanced mRNA expression and STING activation in DCs, improving immunotherapy outcomes in pancreatic cancer. Full article

Aqueous precipitation and crystallization are central to impurity removal, product formation, and resource recovery in mineral and chemical processing, but robust inline monitoring remains challenging because supersaturation is not measured directly and conductivity signals are affected by temperature, composition drift, bubbles, solids, polarization, and fouling. Electrical conductivity (EC) is attractive as a low-cost, rugged process analytical tool, yet its usefulness depends on mechanistic interpretation: EC reflects charge-carrier concentration and mobility rather than supersaturation itself. This review organizes the literature into a layered framework covering (i) measurement integrity and deployment, (ii) bulk-signal extraction in multiphase media, (iii) estimation of latent variables such as dissolved concentration or supersaturation proxies, and (iv) control readiness based on conductivity-derived targets. Frequency-aware conductivity extraction, event-anchored verification, and observer-based estimation are treated as optional, complementary modules. A Ca-carbonate/CaCO₃ system is used as an illustrative case because its coupling among conductivity, pH/speciation, supersaturation, and precipitation is especially transparent, although the framework is intended for broader processing systems, including complex liquors and slurries. Opportunities are also highlighted for nanomaterials to improve both precipitation control and EC information content. Full article

Accurate prediction of overpressured formations in deepwater is important for drilling safety and reservoir evaluation. However, conventional two-step inversion workflows are affected by cumulative errors and parameter crosstalk, which limits their ability to characterize the sharp pressure-transition interfaces at the top of overpressured zones. In this study, we propose a direct prestack nonlinear inversion method for the formation pressure coefficient (λ), a dimensionless and drilling-relevant indicator of overpressure intensity. Unlike previous exact-Zoeppritz direct inversions that target effective stress or elastic moduli, here a single formation pressure coefficient drives the pressure-sensitive rock-physics chain—linking pore pressure, effective stress, and pore-space stiffness to the seismic response—thereby reducing the number of free inversion variables. This single-parameter mapping is then coupled with the exact Zoeppritz equation to build a nonlinear prestack forward operator, helping to reduce the parameter coupling and error propagation associated with conventional multiparameter inversion workflows. To describe the typical blocky structural features of overpressured strata, a nonconvex Lp-norm (0 < p < 1) regularization is introduced as a structural prior, and a decoupled optimization strategy combining the alternating direction method of multipliers (ADMM) and iteratively reweighted least squares (IRLS) is developed for a stable solution. In a single pseudo-well synthetic test, the proposed method achieved a higher correlation coefficient and lower root mean square error (RMSE) than the indirect workflow, indicating improved agreement with the reference formation-pressure-coefficient profile. Application to field seismic data from the Yinggehai Basin, South China Sea, shows that the method produces clearer pressure-transition boundaries and pressure-coefficient profiles more consistent with the available well constraints. These results suggest that, under the tested conditions, the proposed method can provide useful geophysical support for pressure prediction and the characterization of deepwater overpressured reservoirs. Full article

Environmental sustainability has become a critical priority for higher education institutions, which play a key role in promoting awareness and shaping students’ perceptions of sustainable practices. Understanding students’ awareness and perceptions is essential for evaluating the effectiveness of institutional sustainability initiatives. This study aimed to assess students’ awareness and perceptions of environmental sustainability at Prince Sattam bin Abdulaziz University and to examine the influence of demographic factors and the relationship between awareness and perception. A quantitative cross-sectional survey was conducted among 323 university students. Data were collected using a structured questionnaire measuring environmental awareness (18 items) and perception of sustainability practices (14 items) on a 5-point Likert scale. Composite scores were computed as the means of item responses. Descriptive statistics, independent t-tests, one-way ANOVA, and multivariable linear regression analyses were performed. Students demonstrated a moderate level of environmental awareness (mean = 3.116 ± 0.403) and moderate perceptions of sustainability practices (mean = 2.887 ± 0.199). Environmental awareness was significantly higher among female students and those in science-related disciplines (p < 0.001). Perception of sustainability was significantly associated with field of study and level of study (p < 0.001). In multivariable analysis, gender and field of study remained significant predictors of awareness, while gender, field of study, and level of study predicted perception. A significant but negative association was observed between awareness and perception of environmental sustainability (B = −0.496, p < 0.001). While students demonstrated a moderate level of environmental awareness, perceptions of sustainability practices were inconsistent. The findings highlight the need for enhanced sustainability education and engagement initiatives within universities. Future research should explore how awareness and perception translate into meaningful engagement with sustainability practices. Full article

This study evaluated the effects of monolaurin intake per finishing feedlot cattle on growth performance, metabolic status, ruminal environment, and meat fatty acid profile. Twenty-four castrated Holstein males (379 ± 8.5 kg; 12 months old) were randomly assigned to two treatments: basal diet (control) or basal diet with α-monolaurin (treated: 0.762 g/kg dry matter intake; ≈6.63 g/animal/day) for 79 days. Feed intake, body weight, and feed efficiency were recorded, and blood and ruminal samples were collected during the trial. Ruminal fermentation parameters, protozoa counts, hematological and biochemical variables, oxidative status biomarkers, ruminal microbiota composition (16S rRNA sequencing), and Longissimus dorsi fatty acid profile were analyzed. Monolaurin feed did not affect dry matter intake or final body weight, but increased total weight gain, average daily gain, and feed efficiency (p ≤ 0.05), indicating improved nutrient utilization. Hematological and serum biochemical variables were largely unchanged, although total leukocyte counts were lower in treated cattle. Animals receiving monolaurin showed reduced reactive oxygen species and lower superoxide dismutase activity, suggesting improved oxidative balance without changes in lipid peroxidation. During the adaptation phase (day 14), treated cattle exhibited lower acetate, propionate, valerate, and total volatile fatty acid concentrations and higher protozoa counts, but these differences disappeared by day 79, indicating ruminal adaptation. Microbiota diversity was not altered overall, although specific genera differed in relative abundance between treatments. In meat, monolaurin increased lauric, linoleic, and arachidonic acids, reduced palmitic and heptadecanoic acids, decreased total saturated fatty acids, and increased polyunsaturated fatty acids (p ≤ 0.05). Overall, dietary monolaurin improved feed efficiency, modulated oxidative status, induced transient ruminal microbial adjustments, and enhanced the nutritional quality of beef lipids without compromising metabolic health. Full article

Background and Objectives: Quality of life (QoL) is an important issue for breast cancer (BC) survivors. The objective of this study was to assess health-related QoL (HRQoL) of BC patients and investigate the impact of different demographic and clinical factors on physical and social functioning and BC-related symptoms. Materials and Methods: In this cross-sectional study, 107 BC patients undergoing chemotherapy in Greece completed a questionnaire collecting sociodemographic and clinical information and the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire–Core 30 (EORTC QLQ-C30) in order to assess HRQoL. Descriptive statistics and multiple linear regression analyses were used to identify factors linked to HRQoL outcomes. Results: Overall, participants reported moderate HRQoL, with high physical and social functioning and moderate emotional, cognitive, and role functioning. Fatigue was the most common symptom, whereas other symptoms were generally uncommon. Multiple regression analyses showed that marital status, place of residence, time since diagnosis, and type of surgery were significantly associated with the global QLQ-C30 score (R² = 0.337, p < 0.001). Physical functioning was associated with comorbidity burden, time since diagnosis, and employment status (R² = 0.155, p = 0.035), and social functioning with marital status and type of surgery (R² = 0.171, p = 0.011). Emotional functioning showed exploratory associations with place of residence and type of surgery; however, the overall regression model for emotional functioning did not reach statistical significance. No symptom model reached overall significance, but time since diagnosis, treatment type, and surgery were linked to distinct symptoms. Conclusions: BC patients undergoing chemotherapy in Greece report an overall moderate level of HRQoL, which is significantly influenced by a combination of demographic and clinical factors; physical and social functioning were high, with moderate emotional, cognitive, and role functioning. These findings highlight the importance of individualized supportive care strategies in order to improve QoL of BC patients. Full article

Background/Objectives: The objective was to describe the one-year outcomes of salvage trabeculectomy (TLE) in eyes with persistent elevation of intraocular pressure (IOP) requiring early surgical intervention after failed minimally invasive glaucoma surgery (MIGS). Methods: This retrospective observational study included 38 eyes of 38 consecutive Japanese patients who underwent TLE within 100 days after Trabectome (TOM) or microhook ab interno trabeculotomy (μTLO) because of uncontrolled IOP despite maximally tolerated medical therapy. Surgical success was defined as (1) IOP reduction ≥30% from baseline, (2) 5 < IOP < 18 mmHg, (3) no additional glaucoma surgery, and (4) no loss of light perception. The Kaplan–Meier method was used to estimate the one-year success rate. Changes in IOP, medication use, best-corrected visual acuity (BCVA), and mean deviation (MD) were analyzed using the Wilcoxon matched-pairs signed-rank test and a linear mixed-effects model. Results: The median interval between MIGS and TLE was 41.5 days (interquartile range, 28–70 days). The one-year surgical success rate was 86.8% (Kaplan–Meier estimate). IOP and medication use were significantly reduced after TLE (p < 0.0001) and remained stable throughout the 12-month follow-up. BCVA did not differ significantly between baseline and 12 months after TLE, whereas a small but statistically significant difference in MD was observed. No serious vision-threatening complications were encountered. Conclusions: TLE performed shortly after failed MIGS achieved substantial IOP reduction with acceptable safety over a one-year follow-up period. TLE may be considered as one of the surgical options in cases where sufficient IOP reduction cannot be achieved after failed MIGS, and no effective alternative treatments are available. Full article

Phytochemical analysis of the tooth fungus Hydnellum aurantiacum resulted in the isolation of twenty-two compounds, including two new kavalactone derivatives—methylkavain (1) and aurapyrone (2); seven new p-terphenyl derivatives—ethylatromentin (3), 2-O-benzoylatromentin (4), aurantin (5), leucohydnelin (6), leucoaurantin (7), hydroxyleucoaurantiacin (8), benzoyltelephantin M (9); and thirteen known p-terphenyls—atromentin (10), aurantiacin (11), telephantin K (12), leucoatromentin (13), telephantin J (14), curtisian A (15), concrescenin B (16), telephantin L (17), dihydroaurantiacin dibenzoate (18), telephantin M (19), sarcodonin α (20), sarcodonin δ (21) and phellodonin (22). The structures were elucidated using spectroscopic methods (UV, NMR, HR-ESI-MS) along with comparison to literature data. All isolated substances, except 8 and 10, affected thrombin-induced human platelet activation at 90 μM: the seven compounds (9, 12, 16, 17, 20, 21, 22) potentiated it, while the remaining ones exhibited inhibitory activity with the strongest antiplatelet effects observed for 4, 5, and 7 (10.6 ± 4.0, 4.3 ± 3.2, 9.6 ± 2.9% of positive control, respectively). These and other p-terphenyl derivatives with antiplatelet activity identified in this study represent promising structures for further investigation into the mechanism of their action. Full article

Background: Obstructive sleep apnea (OSA) is increasingly recognized as a systemic disorder associated with insulin resistance and elevated risk of type 2 diabetes. While continuous positive airway pressure (CPAP) is the standard therapy, its long-term metabolic benefits remain inconsistent. The metabolic impact of upper airway surgery is less well defined. Methods: In this retrospective study, 49 patients with polysomnography-confirmed OSA who underwent upper airway surgery were evaluated. Respiratory and metabolic parameters—including apnea–hypopnea index (AHI), fasting plasma glucose, fasting insulin, glycated hemoglobin (HbA1c), and homeostatic model assessment for insulin resistance (HOMA-IR)—were assessed preoperatively and at 6 months postoperatively. Associations between changes in AHI (ΔAHI) and insulin resistance (ΔHOMA-IR) were analyzed using correlation and receiver operating characteristic (ROC) analyses. Results: Significant improvements were observed in both respiratory and metabolic parameters. AHI decreased from 46.6 ± 25.8 to 20.7 ± 14.1 events/h (p < 0.001). Fasting plasma glucose, insulin levels, and HOMA-IR were significantly reduced postoperatively (all p < 0.05), while HbA1c showed a downward trend. Reduction in AHI was moderately correlated with improvement in insulin resistance (r = 0.527, p < 0.001). ROC analysis demonstrated modest discriminative ability of ΔAHI for identifying normalization of insulin resistance (AUC = 0.62). Conclusions: Upper airway surgery was associated with significant improvements in insulin resistance and glycemic parameters in patients with OSA. The correlation between airway improvement and metabolic change supports a physiological link between upper airway obstruction and insulin sensitivity. These findings suggest that upper airway surgery may represent a clinically relevant adjunct within multimodal strategies for metabolic risk reduction, particularly in patients unable to tolerate CPAP therapy. Full article