Search Results (77)

Zero-Touch Network (ZTN) represents a cornerstone approach of Next Generation Networks (NGNs), enabling fully automated and AI-driven network and service management. However, their distributed and multi-domain nature introduces critical security challenges, particularly regarding service identity and data integrity. This paper proposes a novel blockchain-based framework to enhance the security of ZTN through two complementary mechanisms: decentralized digital identity management and oracle-assisted network monitoring. First, a Decentralized Identity Management framework aligned with Zero-Trust Architecture principles is introduced to ensure tamper-proof authentication and authorization in a trustless environment among network components. By leveraging decentralized identifiers, verifiable credentials, and zero-knowledge proofs, the proposed Decentralized Authentication and Authorization component eliminates reliance on centralized authorities, while preserving privacy and interoperability across domains. Second, the paper investigates blockchain oracle mechanisms as a means to extend data integrity guarantees beyond the blockchain, enabling secure monitoring of Network Services and validation of Service-Level Agreements. We propose a four-dimensional framework for oracle design, based on qualitative comparison of oracle types—decentralized, compute-enabled, and consensus-based—to identify their suitability for NGN scenarios. This work proposes an architectural and design framework for Zero-Touch Networks, focusing on system integration and security-aware orchestration rather than large-scale experimental evaluation. The outcome of our study highlights the potential of integrating blockchain-based identity and oracle solutions to achieve resilient, transparent, and self-managed network ecosystems. This research bridges the gap between theory and implementation by offering a holistic approach that unifies identity security and data integrity in ZTNs, paving the way towards trustworthy and autonomous 6G infrastructures. Full article

Background: The transition from student to registered nurse is a vulnerable period characterised by emotional strain, role ambiguity, and transition shock. Although Graduate Nurse Transition Programs (GNTPs) aim to strengthen early practice readiness, few evaluations use longitudinal, theory-informed approaches or validated tools. Aim: To examine the professional role development of new graduate nurses (NGNs) across three transition stages within a major Australian health service. Design and Methods: A longitudinal quantitative study guided by Duchscher’s Stages of Transition Theory and the Transition Shock Model. A customised 75-item questionnaire—adapted from the Professional Role Transition Risk Assessment Instrument and the Professional and Graduate Capability Framework—was administered at three transition points (March 2020–March 2021). Four domains were assessed: Responsibilities, Role Orientation, Relationships, and Knowledge and Confidence. Descriptive statistics, Principal Component Analysis (PCA), chi-square tests, and multinomial logistic regression identified developmental patterns and predictors of transition stage. Results: PCA supported a four-factor structure consistent with the theoretical domains, explaining 62% of variance. Significant stage-based improvements were found in clinical decision-making (RS6, p = 0.005), managing pressure (RS11, p = 0.003), leadership perception (RO5, p = 0.001), and emotional regulation (RL20, p < 0.001). Regression analysis identified role confusion (RS7, χ² = 18.112, p = 0.001), leadership potential (RL1, χ² = 25.590, p < 0.001), workplace support (RL16, χ² = 12.760, p = 0.013), and critical thinking confidence (KN13, χ² = 10.858, p = 0.028) as strong predictors of transition stage. By Stage 3, most NGNs demonstrated increased autonomy, confidence, and professional integration. A coordinator-to-graduate ratio of 1:12 facilitated personalised mentorship. Conclusions: Findings provide robust evidence for theoretically grounded GNTPs. Tailored interventions—such as early mentorship, mid-stage stress support, and late-stage leadership development—can enhance role clarity, confidence, and workforce sustainability. Full article

Background/Objectives: Flavonoids have been extensively investigated as reducing and stabilizing agents in the green synthesis of metallic nanoparticles. However, studies specifically employing pure naringin (NG) and naringenin (NGN) remain relatively scarce. Methods: In the present work, silver nanoparticles (AgNPs) were synthesized under controlled laboratory conditions using NG and NGN as bioreductants, and critical parameters governing nanoparticle formation were optimized. The synthesized AgNPs were comprehensively characterized using ultraviolet–visible (UV–Vis) spectroscopy, dynamic light scattering (DLS), scanning transmission electron microscopy (STEM), energy-dispersive X-ray spectroscopy (EDX), and Fourier-transform infrared spectroscopy (FTIR). Results: The characterization analyses confirmed the successful formation of predominantly spherical AgNPs with average particle sizes of 17 nm (AgNG) and 20.4 nm (AgNGN). DLS analysis indicated zeta potentials of approximately −30 mV and PDIs of 0.45 (AgNG) and 0.29 (AgNGN), consistent with stable colloidal dispersions. Biological evaluations revealed that both AgNP systems exhibited notable antioxidant and antimicrobial activities. Furthermore, cytogenetic assessment using the Allium cepa assay demonstrated concentration-dependent alterations in mitotic index and chromosomal integrity, indicating biological activity at cellular level. Conclusions: Collectively, these results underscore the potential of flavonoid-mediated synthesis as an eco-friendly and effective approach for generating stable, bioactive nanomaterials with promising biological applications. Full article

Airway obstruction may lead to dentofacial dysmorphogenesis, with severity influenced by age, duration, and extent of obstruction. Aims: to evaluate long-term craniofacial changes in children with a history of mouth breathing, comparing outcomes between those treated with lymphoid tissue removal or with medication, and considering treatment age. Materials and Methods: Fifty-seven patients with a mean age of 19.09 years (range: 15.1–25.2 years) who had been evaluated in an earlier study (T1) were recalled at an average of 13 years follow-up (T2) and classified into a surgical group (n = 34), who had an adenoidectomy, and a non-surgical group (n = 23) treated with medication. Lateral cephalograms were obtained and compared with the original pre-treatment records. Control groups were included, matching the subjects in both groups for age and sex. Statistical analyses included group comparisons and associations among variables. Results: Significant improvement in both treatment groups were observed for the gonial angle (Ar-Go-Me), facial convexity (S-N-Me) and facial height (N-Gn), but T2-T1 changes in the surgical group were statistically significantly greater than in the medical therapy group. The palatal plane inclination to the horizontal (PP/H) and the mandibular plane inclination (MP/H) and to cranial base (MP/SN) were significantly improved in both groups (0.001 < p < 0.01). Conclusions: Both surgical and medical treatment of airway obstruction resulted in the reversal of the harmful effect of the obstruction. However, adenoidectomy was associated with greater improvements, possibly because the original obstruction was more severe and longer-standing. The results underline the importance of early recognition and management of airway obstruction to mitigate developmental orofacial dysmorphology. Full article

Sodium metal batteries (SMBs) with ceramic solid electrolytes offer a promising route to improve the energy density of conventional Na-ion batteries (SIBs). Silicate-based ceramics have recently gained attention for their favourable properties, including better ionic conduction and wider stability windows. In this study, 10% Pr³⁺ and Nd³⁺ were doped into sodium gadolinium silicate ceramics to examine the effects on phase purity, ionic conductivity, and interfacial compatibility with sodium metal anodes. The materials were synthesized via solid-state methods and sintered at 950–1075 °C to study the impact of sintering temperature on densification and microstructure. Na₅Gd_0.9Pr_0.1Si₄O₁₂ (NGPS) and Na₅Gd_0.9Nd_0.1Si₄O₁₂ (NGNS) sintered at 1075 °C showed the highest room temperature total ionic conductivities of 1.64 and 1.74 mS cm⁻¹, respectively. The highest critical current density of 0.5 mA cm⁻² is achieved with a low interfacial area-specific resistance of 29.47 Ω cm² for NGPS and 22.88 Ω cm² for NGNS after Na plating/stripping experiments. These results highlight how doping enhances phase purity, ionic conductivity, and interfacial stability of silicates with Na metal anodes. Full article

With the increasingly frequent exploitation and transportation of offshore oil, the threat of oil spill accidents to the marine ecological environment has become increasingly serious. It is urgent to develop efficient and reliable oil film monitoring technology. Based on the marine radar oil spill data, an innovative OAT-NGN hybrid strategy segmentation algorithm was proposed. By integrating the local feature learning ability of a Neural Gas Network (NGN) and the global search strategy of the Oat optimization algorithm (OAT), the proposed method effectively meets the challenges of traditional oil film segmentation methods in complex sea conditions. Firstly, the raw data of marine radar were preprocessed by using co-frequency interference and speckle noise suppression. Then, the OAT algorithm guided the updating of neural weights in the NGN on a global scale for the exploration of a more optimal solution space during the optimization process. Finally, the oil spill segmentation results were projected to the polar coordinate system through post-processing technology. The experimental results showed that this method effectively balanced the problem of false detection and missing detection. Compared with existing methods, OAT-NGN shown stronger adaptability in complex scenarios. In order to improve the segmentation performance, its innovative dynamic weight adjustment mechanism and spatial constraint design provide a new technical path. Full article

Background/Objectives: This study protocol outlines the implementation of a professional development programme (ProDeveloP) designed to support newly graduated nurses (NGNs). NGNs often experience inadequacy and face challenges in developing their professional competence. Healthcare organisations can ease this transition through introduction programmes. However, effective implementation strategies in local healthcare settings remain unclear. This study aims to develop, implement, and evaluate a programme that enhances role clarity, task mastery, social acceptance, stress reduction, and recovery, contributing to increased job satisfaction and retention. Methods: ProDeveloP will be implemented at a regional hospital in mid-Sweden. This study will include a convenience sample of 110 NGNs from both the previous and the new introduction programmes, 20 dialogue facilitators involved in the programme, and 10 managers responsible for NGNs participating in the programme. Data will be collected throughout the ProDeveloP using questionnaires, individual interviews, and focus groups. The data will be analysed using both qualitative and quantitative analyses. Conclusions: This study has the potential to improve NGNs’ work-related health, job satisfaction, and retention while contributing to competence development. By offering structured reflection, mentorship, and organisational support, this research project addresses knowledge gaps in understanding the long-term impacts of introduction programmes and structured reflection, contributing to evidence-based improvements in nursing practice and healthcare leadership. Clinical trial number: NCT06742047. Registered in ClinicalTrials.gov, 19 December 2024. Full article

The presence of pesticides in surface waters has been widely reported worldwide and represents a significant problem that raises concerns on local, regional, national, and international scales. Among these, metolachlor is one of the most widely used herbicides to control annual grasses and broadleaf weeds in various crops. Despite the existing research, data on the effects of metolachlor on the nervous system of fishes, remain limited. The present study aims to investigate the impact of metolachlor during embryonic development on the formation of the nervous system and the subsequent inflammatory response in zebrafish (Danio rerio), focusing specifically on larvae at 24 h post-fertilization (hpf). To achieve this, transgenic zebrafish lines marking neuronal populations Tg(Hu:GFP), glial cells Tg(gfap:GFP), and circulating macrophages Tg(mpeg:GFP) were employed. Following exposure to sub-lethal doses of metolachlor, we observed a significant decrease in GPF-positive cells marking the neuronal population, accompanied by an increase in apoptotic cells within the brain region. Additionally, treated embryos exhibited a marked neuroinflammatory response, characterized by astrogliosis and the specific accumulation of microglia/macrophage-positive cells in the head region. In situ hybridization and real-time PCR analyses revealed a significant downregulation of the neurogenin-1 (ngn1) transcript and a noticeable upregulation of the pro-inflammatory cytokine interleukin-1 beta (il1b). Our findings contribute to the growing body of evidence suggesting that metolachlor, even at early developmental stages, can have detrimental effects on both the formation of the nervous system and the regulation of immune responses. Full article

Bisphenol A diglycidyl ether (BADGE) is the main component of epoxy resin and is used for the inner coating of canned foods and plastic food containers. BADGE can easily migrate from containers and result in food contamination; the compound is known as an endocrine-disrupting chemical. We previously reported that maternal exposure to bisphenol A bis (2,3-dihydroxypropyl) ether (BADGE·2H₂O), which is the most detected BADGE derivative not only in canned foods but also in human specimens, during gestation and lactation, could accelerate neuronal differentiation in the cortex of fetuses and induce anxiety-like behavior in juvenile mice. In this study, we investigated the effects of low-dose BADGE·2H₂O (1–100 pM) treatment on neurites and the mechanism of neurite outgrowth in cortical neurons. BADGE·2H₂O exposure significantly increased the number of dendrites and neurite length in cortical neurons; these accelerating effects were inhibited by estrogen receptor (ER) antagonist ICI 182,780 and G-protein-coupled estrogen receptor (GPER) antagonist G15. BADGE·2H₂O down-regulated Hes1 expression, which is a transcriptional repressor, and increased levels of neuritogenic factor neurogenin-3 (Ngn3) in the cortical neurons; the changes were significantly blocked by G15. These data suggest that direct BADGE·2H₂O exposure can accelerate neuritogenesis and outgrowth in cortical neurons through down-regulation of Hes1 and by increasing Ngn3 levels through ERs, particularly GPER. Full article

The neuronal progenitor NG108-15 neuroblastoma x glioma cell line proliferates indefinitely in vitro and is capable of directed differentiation into cholinergic neurons. The cell line is a robust model for investigating neuronal differentiation and function in vitro. The lineage-specific transcription factor-mediated differentiation of pluripotent stem cell lines (PSCs) leads to more rapid, efficient, and functional neurons. In this study, we tested the hypothesis that transcription factors could also drive the fate of an immortalised cell line. We first established a stable NG108-15 cell line, by piggyBac (pBac) transposition, that conditionally expresses neurogenin-2 (Ngn2), a common transcription factor for specifying neuronal fate. Following doxycycline-induction of Ngn2, we observed more rapid and efficient differentiation, and improved neurite outgrowth and viability compared with the WT cell line. Moreover, when co-cultured with C2C12 mouse myotubes, the modified NG108-15 cells resulted in significantly larger acetylcholine receptor (AChR) aggregates, suggesting enhanced neuromuscular junction (NMJ) formation. These findings describe a novel methodology for differentiating NG108-15 cells more efficiently, to enhance the usefulness of the cell line as a motor neuron model. Full article

Intensified complementary metal-oxide semiconductor (ICMOS) sensors involve multiple steps, including photoelectric conversion and photoelectric multiplication, each of which introduces noise that significantly impacts image quality. To address the issues of insufficient denoising performance and poor model generalization in ICMOS image denoising, this paper proposes a systematic solution. First, we established an experimental platform to collect real ICMOS images and introduced a novel noise generation network (LD-NGN) that accurately simulates the strong sparsity and spatial clustering of ICMOS noise, generating a multi-scene paired dataset. Additionally, we proposed a new noise evaluation metric, KL-Noise, which allows a more precise quantification of noise distribution. Based on this, we designed a denoising network specifically for ICMOS images, MAST-Net, and trained it using the multi-scene paired dataset generated by LD-NGN. By capturing multi-scale features of image pixels, MAST-Net effectively removes complex noise. The experimental results show that, compared to traditional methods and denoisers trained with other noise generators, our method outperforms both qualitatively and quantitatively. The denoised images achieve a peak signal-to-noise ratio (PSNR) of 35.38 dB and a structural similarity index (SSIM) of 0.93. This optimization provides support for tasks such as image preprocessing, target recognition, and feature extraction. Full article

New graduate nurse (NGN) turnover is emerging as one of the foremost issues in healthcare systems, primarily due to the implications for patient care and the need to secure the human resource future of the nursing profession. The initial months of transitioning into the professional role are crucial for cultivating and developing clinical practice patterns, professional values and a connection to the profession. However, the initial transition period for new nurses is associated with numerous challenges that can interrupt a healthy introduction into practice, justifying the critical prioritization of these issues. In light of these challenges to NGN entry to practice, this paper aims to conceptualize the contemporary professional role transition experiences of new graduate nurses and highlight the potential leverage that transition theories offer in managing this experience. Eleven transition theories relevant to this discourse were identified to enhance the understanding and comprehension of the new graduate nurses to inform future initiatives, directives, interventions and policies. Full article

Introduction: The variants rs10517086 and rs1534422 are predictive of type 1 diabetes mellitus (T1DM) development and poor residual β cell function within the first year of diagnosis. However, the mechanism by which risk is conferred is unknown. We explored the impact of both variants on β cell function in vitro and assessed their relationship with C-peptide in people with T1DM and type 2 diabetes mellitus (T2DM). Methods: Using CRISPR/Cas9, the variants were introduced into a β cell line (BRIN-BD11) and a T cell line (Jurkat cells) from which the conditioned media was applied to otherwise healthy β cells to model the inflammatory environment associated with these variants. Results: Both variants significantly reduced glucose-stimulated insulin secretion, increased production of pro-inflammatory cytokines and reduced expression of several β cell markers and transcription factors (KCNJ11, KCNQ1, SCL2A2, GCK, NKX6.1, Pdx1 NGN3). However, HNF1A was significantly upregulated in the presence of both variants. We subsequently silenced HNF1A in variant expressing BRIN-BD11 cells using siRNA and found that gene expression profiles were normalised. Induction of each variant significantly increased expression of the lncRNAs they encode, which was normalised upon HNF1A silencing. Analysis of the DARE (Diabetes Alliance for Research in England) study revealed an association of rs10517086_A genotype with C-peptide in 153 individuals with T1DM, but not in 417 people with T2DM. Conclusions: These data suggest that rs1534422 and rs10517086 exert multiple insults on the β cell through excessive upregulation of HNF1A and induction of pro-inflammatory cytokines, and highlight their utility as prognostic markers of β cell function. Full article

Background/Objectives: The literature suggests that the cranial base angle is considered one of the contributing factors to sagittal jaw malpositions when its relationship with the viscerocranium is examined. Our study aims to compare and evaluate the outcomes of fixed functional orthopedic treatment in patients with mandibular retrognathia across different cranial base groups. Methods: Participants were treated at Erciyes University with fixed functional appliances and categorized by CBA into low (<130°), medium (130°–134°), and high (>134°) groups. A total of 39 patients were included: 13 in the low CBA group (7 males, 6 females; mean age 14.62 ± 1.12 years), 13 in the medium CBA group (3 males, 9 females; mean age 14.38 ± 0.96 years), and 13 in the high CBA group (4 males, 9 females; mean age 14.08 ± 1.04 years). Results: In the low CBA group, Ar-Go-N (p = 0.005) and SNA (p = 0.023) angles significantly decreased, while the ANB angle and Wits appraisal significantly decreased across all groups (p < 0.05). The high CBA group showed increases in ANS-Me, N-Me, N-ANS, and N-Gn lengths (p < 0.05). The medium and high CBA groups had significant increases in S-Go and ANS-Gn lengths, while Co-Gn length increased significantly in the low and high CBA groups (p < 0.05). Incisor measurements (IMPA, L1-APog, L1/NB, L1-NB) increased in all groups, with overjet and overbite reduced (p < 0.05). U1/PP (p = 0.039), U1/SN (p = 0.043), U1-NA (p = 0.030), and U1/NA (p = 0.025) parameters increased in the low CBA group, with the Upper Lip–E distance decreasing significantly in the low and high CBA groups (p < 0.05). A comparison between groups showed significant differences in U1/PP, U1-NA, and U1/NA parameters, which increased in the low and medium CBA groups but decreased in the high CBA group. Conclusions: CBA influences treatment outcomes. The low CBA group experienced decreases in Ar-Go-N and SNA angles, while the high CBA group showed increases in certain vertical facial dimensions. Incisor parameters rose in the low and medium CBA groups but decreased in the high CBA group, suggesting limited CBA effects on treatment results. Full article