Search Results (9)

A topological analysis was performed by taking ESEEM measurements of site-specifically labeled E protein from SARS-CoV-2. The intensity of deuterium modulation arising from either deuterated solvent or deuterated lipid acyl chains revealed exposure to solvent or the bilayer hydrophobic region. Spin-labeled lipids and soluble spin labels were used as points of comparison. The data indicate that spin labels placed along the transmembrane helix of the E protein showed close contact with lipid acyl chains, but also substantial contact with solvent, while those placed on the C-terminal domain showed substantial but lower exposure to lipid acyl chains, with comparable solvent exposure. The results support the view that the C-terminal domain is in contact with the bilayer surface. Full article

Epigallocatechin gallate (EGCg), an abundant phytochemical in green tea, is an antioxidant that also binds proteins and complex metals. After gastrointestinal absorption, EGCg binds to serum albumin in the hydrophobic pocket between domains IIA and IIIA and overlaps with the Sudlow I site. Serum albumin also has two metal binding sites, a high-affinity N-terminal site (NTS) site that selectively binds Cu(II), and a low-affinity, less selective multi-metal binding site (MBS). We proposed to determine whether EGCg binds or reacts with Cu(II)-serum albumin using fluorescence, UV–Visible and electron paramagnetic resonance (EPR) spectroscopy. Our results suggest that when serum albumin is loaded with Cu(II) in both sites, EGCg binds to the MBS-Cu(II) and reduces the copper to Cu(I). EGCg does not bind to or react with Cu(II) in the high-affinity NTS site. Potential consequences include changes in copper homeostasis and damage from pro-oxidative Fenton reactions. Full article

Zambia’s adult HIV prevalence is high at 11% and faces challenges in achieving UNAIDS 95-95-95 targets for HIV, with a national viral load suppression of 86.2% falling short of the required 95%. North-Western Province has the lowest viral load suppression at 77.5%. Our study investigated the role of an integrated sample referral system in optimizing HIV viral load coverage and Early Infant Diagnosis turnaround time in the province. Using electronic data from the DISA Laboratory Information System and Smartcare, a retrospective cross-sectional analysis was conducted, involving 160,922 viral load and Early Infant Diagnosis results. The chi-square test and multiple linear regression were used for analysis. Following the implementation of the integrated sample referral system, viral load coverage consistently increased monthly (p < 0.001), Early Infant Diagnosis turnaround time improved by 47.7%, and sample volume increased by 25%. The study identifies associations between various factors and testing outcomes. These findings demonstrate improvements in viral load coverage and the Early Infant Diagnosis turnaround time and suggest targeting modifiable factors to further optimize the referral system. We recommend continued strengthening of the referral system and more deliberate demand-creation implementation strategies. Full article

Social Network Analysis (SNA) has shed light on cultures where the influence of patronage, preferment, and reciprocal obligations are traditionally important. We argue here that episcopal appointments, culture, and governance within the Catholic Church are ideal topics for SNA interrogation. This paper presents preliminary findings, using original network data for the Catholic Bishops’ Conference of England and Wales and the United States Conference of Catholic Bishops. These show how a network-informed approach may help with the urgent task of understanding the ecclesiastical cultures in which sexual abuse occurs, and/or is enabled, ignored, and covered up. Particular reference is made to Theodore McCarrick, the former DC Archbishop recently “dismissed from the clerical state”, and Michael Bransfield, Bishop Emeritus of Wheeling-Charleston. Commentators naturally use terms such as “protégé”, “clique”, “network”, and “kingmaker” when discussing both the McCarrick and Bransfield affairs, and church politics more generally: precisely such folk-descriptions of social and political life that SNA is designed to quantify and explain. Full article

Rumination and worry, collectively referred to as perseverative cognition, have been implicated in the increased engagement of several health risk behaviours. The current study aimed to investigate the potential influencing role of these repetitive negative thought cognitions in an online snack paradigm. Participants were randomly assigned to either an even condition (a 3:3 ratio of ≤101 kcal and >201 kcal snacks) or an uneven condition (a 4:2 ratio in favour of ≤101 kcal snacks). Upon the presentation of six images of sweet treats, participants were asked to choose the snack they most wanted to consume “right now”, before completing the Ultra-Brief Penn State Worry Questionnaire (UB-PSWQ) and the brief (5-item) Ruminative Response Scale (RRS). The results showed that the reduced availability of higher calorie snacks significantly improved both snack choice and total calorie consumption. However, despite rumination and worry having no influence on the snack type chosen, higher levels of rumination still led to significantly higher overall calorie consumption. Although, contrary to predictions, higher levels of worry conversely led to significantly lower overall calorie consumption. This study adds to the growing work in the role of perseverative cognition and food consumption, which may aid in informing public health strategies. Further exploration is needed to assess whether rumination directly induces unhealthy eating behaviours or simply exacerbates them. Full article

We discuss results on plasma density profile modifications in the F-region ionosphere that are caused by HF heating with the frequency f₀ in the range [(−150 kHz)–(+75 kHz)] around the fourth electron gyroharmonic 4f_c. The experiments were conducted at the HAARP facility in June 2014. A multi-frequency Doppler sounder (MDS), which measures the phase and amplitude of reflected sounding radio waves, complemented by the observations of the stimulated electromagnetic emission (SEE) were used for the diagnostics of the plasma perturbations. We detected noticeable plasma expulsion from the reflection region of the pumping wave and from the upper hybrid region, where the expulsion from the latter was strongly suppressed for f₀ ≈ 4f_c. The plasma expulsion from the upper hybrid region was accompanied by the sounding wave’s anomalous absorption (AA) slower development for f₀ ≈ 4f_c. Furthermore, slower development and weaker expulsion were detected for the height region between the pump wave reflection and upper hybrid altitudes. The combined MDS and SEE allowed for establishing an interconnection between different manifestations of the HF-induced ionospheric turbulence and determining the altitude of the most effective pump wave energy input to ionospheric plasma by using the dependence on the offset between f₀ and 4f_c. Full article

The Catholic Church is becoming a waning influence in global civil society. This is due, in part, to demographic changes that show an increasing loss of adherents within the Church’s traditional strongholds. Coupled with the growth of liberal social policies and continuing revelations about the crimes of sexual abuse by its clergy, the Church is being forced to reconsider how to continue as a moral advocate in civil society. It has sought to do this by recalibrating its position in global church-state relations, moving toward a non-ideological or “third way” of politics, and seeking non-partisan solutions to social justice needs. However, even this shift has not been sufficient to address the erosion of the Church’s positive, political influence globally. For the Church to be successful in this goal, it will be necessary to totally re-set its social agenda as well as its religious priorities. Such tasks, however, will be difficult at best and almost impossible to accomplish where the primary obstacle for successful political efficacy and internally meaningful change is the Church’s own mismanagement of its two-millennia-old ecclesiastical structure. Full article

Perseverative cognition (PC), consisting of worry and rumination, has been consistently linked to a variety of poorer health outcomes, namely via the worsening of stress-induced health risk behaviours. However, research into PC and unhealthy food choice, a key health behaviour, still remains relatively unexplored. In the current pilot investigation, 284 participants were recruited to take part in an online food choice paradigm before completing the Perseverative Thinking Questionnaire (PTQ) and the Brief State Rumination Inventory (BSRI). As a reduced availability of unhealthy snacks has been shown to improve snack choice, participants were randomly allocated to either an even condition (a 3:3 ratio of ≤99 kcal and ≥199 kcal snacks) or an uneven condition (a 4:2 ratio in favour of ≤99 kcal snacks). It was hypothesized that higher levels of PC may predict greater instances of poorer snack choices across, or even within, this paradigm. Despite an increase availability of lower calorie snacks leading to a healthier snack choice, both state and trait PC measures did not significantly influence snack choice irrespective of this varying availability. Although, marginal trends were found for higher state PC and higher calorie crisp selections. The current pilot therefore adds to the growing literature advocating for the use of behavioural economic tactics to engender healthier food choices, yet further work is needed to unpick the mediating role of PC (and its components) in snack consumption paradigms. Full article

The increased use of nanoparticles (NPs) requires efficient testing of their potential toxic effects. A promising approach is to use reporter cell lines to quickly assess the activation of cellular stress response pathways. This study aimed to use the ToxTracker reporter cell lines to investigate (geno)toxicity of various metal- or metal oxide NPs and draw general conclusions on NP-induced effects, in combination with our previous findings. The NPs tested in this study (n = 18) also included quantum dots (QDs) in different sizes. The results showed a large variation in cytotoxicity of the NPs tested. Furthermore, whereas many induced oxidative stress only few activated reporters related to DNA damage. NPs of manganese (Mn and Mn₃O₄) induced the most remarkable ToxTracker response with activation of reporters for oxidative stress, DNA damage, protein unfolding and p53-related stress. The QDs (CdTe) were highly toxic showing clearly size-dependent effects and calculations suggest surface area as the most relevant dose metric. Of all NPs investigated in this and previous studies the following induce the DNA damage reporter; CuO, Co, CoO, CdTe QDs, Mn, Mn₃O₄, V₂O₅, and welding NPs. We suggest that these NPs are of particular concern when considering genotoxicity induced by metal- and metal oxide NPs. Full article