Search Results (54)

Prolonged drought and rapid land-use transformation are reshaping peasant farming systems worldwide, particularly in regions exposed to extractive agribusiness expansion. This study examines how socio-ecological resilience varies across farming systems differentiated by ethno-cultural background under Chile’s megadrought (2009–2019) in the Araucanía Region. We conducted a longitudinal assessment of 78 smallholder farms (30 Mapuche, 30 Chilean, 18 European descent) using a resilience index integrating vulnerability (water access, proximity to exotic forest plantations, cultivated homogeneity) and response capacity (drought-resistant crops, knowledge and preventive practices for dealing with water deficit, social networks). The results show that Mapuche farming systems consistently exhibited higher resilience, associated with greater cultivated diversity, a lower presence of neighboring forest plantations, and greater knowledge of how to deal with drought events. In contrast, non-Mapuche systems displayed higher vulnerability indicators linked to increased cultivated homogeneity. Over the 10-year period, 32% of the farms included in this study collapsed, primarily due to conversion to exotic forest plantations, disproportionately affecting European-descent and Chilean farms. The higher permanence of Mapuche farms demonstrates that resilience is not solely determined by climatic exposure but is strongly mediated by ethno-cultural land-use practices and socio-ecological memory. The interaction between the megadrought and exotic forest plantations-driven landscape homogenization accelerates differential system persistence. Strengthening agroecological diversity and recognizing culturally embedded agricultural management practices are critical for sustaining resilient farm systems under climate change. Full article

This study examines how Mapuche educational values are incorporated and reinterpreted within Early Childhood Education in Lafkenche contexts. Using a qualitative intercultural approach, research was conducted in three kindergartens in Saavedra, involving children, families, kimches (wise community members), and Indigenous Language and Culture Educators (ELCI). The findings reveal that values such as kümeche (good person), norche (upright person), kimche (wise person), and newenche (strong person) are transmitted through play, storytelling, autonomy, songs, and rituals. Despite these advances, structural gaps remain, such as limited intercultural teacher training, lack of curricular guidance, and weak school–family articulation. The study concludes that moving toward truly intercultural early education demands recognizing kimün (Mapuche wisdom) as a valid source of knowledge and strengthening pedagogical connections between schools, families, and local communities. Full article

The forestry extractivist model has systematically transgressed and violated Mapuche territories, thereby generating tensions, crises, and socioenvironmental injustices. The following objectives were proposed: (a) Unveil the implications of forestry extractivism on bodies of water in rural Mapuche territories. (b) Investigate Mapuche knowledge, sociocultural activities, and their relationship with preservation and sustainability of water in Mapuche and non-Mapuche territories. A qualitative methodology was employed, framed within constructivist grounded theory. To collect the information, in-depth interviews and participant observation were used. The study subjects corresponded to 51 kimeltuchefes (People with knowledge, experience and ancestral wisdom). Regarding objective (a), the findings reveal that pine and eucalyptus forestry extractivism has considerably deteriorated natural (sacred) spaces and the soil. Along with this, it has caused water scarcity, which in turn has reduced medicinal plant and food production and, in general, has deteriorated the ixofil mogen (a concept similar to biodiversity). It was concluded that the forestry extractivist model threatens the existence of all forms of life that cohabit in nature (material and immaterial); it deteriorates Mapuche culture; likewise, it poses a considerable risk to the health and survival of the Mapuche population. Regarding objective (b), the findings reveal that the knowledge of az mapu, ngülam, pepilkantün, rakizuam, llellipun and kümelkawün, and the sociocultural activities, trawün and kelluwün, constitute essential contributions for the preservation and sustainability of water. These forms of knowledge and activities are founded on ethical and moral principles that underlie the normative, legal, social, and educational frameworks of the Mapuche people. It was concluded that sociocultural knowledge and activities are essential for conserving and ensuring water in a sustainable, equitable, and efficient manner for both the Mapuche and non-Mapuche populations and for all life; likewise, they safeguard and promote Mapuche culture. Indeed, these forms of knowledge and sociocultural activities must be incorporated into environmental public policies. Full article

This study aimed to adapt and psychometrically validate the Socio-Educational and Cultural Ambivalence Scale (EASC) in the context of Chilean intercultural education, considering teachers, students, and parents/caregivers. Socio-educational ambivalence is defined as the coexistence of contradictory beliefs, emotions, and practices in the relationship between dominant school knowledge and Mapuche educational knowledge. Using a sequential mixed qualitative–quantitative design, we conceptually reviewed the original instrument and administered the adapted version to a sample of 739 participants (266 teachers, 286 students, and 183 parents/caregivers) from the regions of Biobío, La Araucanía, and Los Lagos. We proposed two six-factor scales: one shared by adults (teachers and parents/caregivers) and another with the same structure but fewer indicators for students. Confirmatory factor analysis (CFA) showed good model fit for both teachers and parents/caregivers (χ² = 1100.85, df = 311, p < 0.001; RMSEA = 0.075; SRMR = 0.058; CFI = 0.934; TLI = 0.926) and students (χ² = 378.546, df = 146, p < 0.001; RMSEA = 0.074; SRMR = 0.033; CFI = 0.978; TLI = 0.974). Composite reliability coefficients were ω = 0.702–0.974 for adults and ω = 0.749–0.948 for students. The results support factorial validity, internal consistency, and scalar invariance for the adult category of the instrument (teachers and parents/caregivers), confirming its usefulness for assessing epistemic and cultural tensions in intercultural educational contexts. The EASC contributes to the development of tools that foster a more plural, reflective, and context-sensitive understanding of education in Indigenous territories. Full article

Berries are an excellent source of bioactive compounds, particularly polyphenols, and have been widely used in folk medicine by the Mapuche people of southern Chile. In this study, a hydroalcoholic extract of Berberis congestiflora Gay (BE) was analyzed to determine its phytochemical composition and to evaluate its antioxidant capacity, vasorelaxant effects in rat aortas, and inhibitory activity on enzymes related to chronic non-communicable diseases, including exploration of a possible vasodilatory mechanism in isolated rat aortas. Antioxidant activity was assessed using Oxygen Radical Absorbance Capacity (ORAC), DPPH (2,2-diphenyl-1-picrylhydrazyl), and ABTS (2,2-azinobis-(3-ethylbenzothiazolin-6-sulfonic acid)) radical scavenging assays, as well as ferric reducing antioxidant power (FRAP). Vascular responses to the Berberis extract were studied using isometric tension recordings in an ex vivo rat thoracic aortic ring model, and the chemical constituents of BE were identified for the first time by HPLC-DAD-MS. The extract itself produced a dose-dependent contraction at 100 and 1000 µg/mL and induced relaxation in phenylephrine-precontracted aortas at the same concentrations, with a maximum contraction of 71% and maximum relaxation of 70% at 1000 µg/mL. Mechanistically, the extract triggered calcium-mediated contraction primarily through calcium release from the sarcoplasmic reticulum and, to a lesser degree, via extracellular Ca²⁺ influx, while its relaxant effect depended on an intact endothelium and activation of the NO/cGMP pathway. In addition, the extract showed inhibitory activity against cholinesterase, glucosidase, and amylase, with IC₅₀ values of 7.33 ± 0.32, 243.23 ± 0.3, and 27.21 ± 0.03 µg/mL, respectively, and docking studies were carried out for selected berry compounds. Overall, these findings indicate that these berries are a rich source of bioactive constituents with antioxidant properties and endothelium-dependent vasodilator effects, supporting their traditional use and highlighting their potential as enzyme inhibitors and as promising candidates for the development of phytotherapeutic products, particularly as supplements for chronic disease management. Full article

Schools situated in indigenous territories have historically replicated Western Eurocentric spatial models, often excluding local cultural knowledge and practices. This exclusion has impacted students’ emotional well-being, learning quality, and the contextual relevance of pedagogical approaches. This study aims to explores the socio-ecological context of school spaces in Mapuche territories in La Araucanía, Chile, and examines how teachers perceive these spaces and their influence on emotional regulation and learning. Using a qualitative multi-case study design, data were collected from three schools in Cholchol, Nueva Imperial, and Toltén through land cover/use mapping within a 3 km radius and semi-structured interviews with 15 teachers. Analysis was conducted using constructivist grounded theory. The findings reveal that schools are embedded in landscapes comprising agricultural zones, water bodies, monoculture plantations, and nearby Mapuche communities. Teachers conceptualize school spaces beyond physical infrastructure, recognizing socio-ecological and cultural dimensions. However, school design remains predominantly Western and monocultural, with limited integration of Mapuche spiritual and territorial elements. The main contribution of this research is to provide empirical evidence that strengthening the connection between school spaces and their biocultural context can enhance students’ sense of belonging, emotional regulation, and learning. This study advances the topic by highlighting the critical role of teachers’ perceptions and the socio-ecological context in the design of intercultural and sustainable schools, offering a concrete framework for overcoming regulatory and architectural limitations that continue to impede the implementation of inclusive public policies in indigenous educational settings. Full article

Quitral (Tristerix corymbosus), a Chilean and Argentine parasitic mistletoe, is traditionally used by Mapuche natives to treat stomach ulcers, nervous disorders, and cholesterol reduction, although scientific support is scarce. Methanolic and chloroform extracts from its leaves and stems were prepared. Chemical analysis included antioxidant capacity assays (ORAC-FL and DPPH) and chromatographic determinations. The antimicrobial activity was tested against nine bacteria and two yeast strains. Additionally, cytotoxicity (hemolysis) and toxicity (against Caenorhabditis elegans) assays were performed. The results revealed that the methanolic leaf extracts had the highest ORAC-FL value, with DPPH assays showing solvent-dependent differences. Thirty-one compounds were tentatively identified, of which 61% were phenolic compounds, primarily flavonoids like quercetin and its derivatives. Antimicrobial results showed activity against Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, and Enterococcus faecalis), but not against yeast Candida guillermondii and Candida tropicalis. Methanolic extracts induced dose-dependent erythrocyte hemolysis, while chloroform extracts showed no relevant cytotoxicity. Toxicity against Caenorhabditis elegans was also dose-dependent for methanolic extracts; leaf extract reduced survival at 50 mg mL⁻¹ after 24 h. These findings partially validate some traditional uses, highlight the importance of solvent polarity in extraction and biological effects, and establish quitral as a flavonoid source. Full article

Skin cancer is increasing worldwide, with melanoma being its most aggressive and lethal form due to its high metastatic potential. Despite therapeutic advances, drug resistance remains a challenge, highlighting the need to explore new anticancer agents. Leptocarpha rivularis is a native plant of Chile, locally called “Palo negro”, and is traditionally used in medicine by the Mapuche people. L. rivularis produces bioactive germacrene sesquiterpenoids with cytotoxic, antioxidant, anti-inflammatory and anti-angiogenic properties. This study reports for the first time the isolation of ovatifolin from aerial parts of L. rivularis and its identification by NMR and X-ray diffraction, together with its antiproliferative activity against two melanoma cell lines. The results show that ovatifolin has cytotoxic activity against the cell lines A2058 and A375, with an IC₅₀ of 27.6 (90.2 µM) and 18.4 µg/mL (60.1 µM), respectively, evaluated by live-cell IncuCyte^® analysis. Moreover, ovatifolin arrests colony formation in a clonogenic assay, with an IC₅₀ of 3.26 (10.6 μM) and 3.65 µg/mL (11.9 μM) in these same cell lines. Therefore, ovatifolin increased intracellular ROS and decreased the mitochondrial membrane potential (ΔΨ m). Cell death studies using Annexin V showed that its cytotoxic activity is partially caused by non-specific apoptosis, which was corroborated by the caspase inhibitor Z-VAD with an incomplete recovery of the cell death process. Full article

Introduction: Engaging social actors dedicated to education has facilitated dialogue, emotional connection, action, and the accumulation of knowledge to create insights that address community needs and promote its development. This study aims to understand the relationship between education, culture, and community as described by teachers working in Mapuche contexts in the Araucanía region of Chile. Method: This research was based on social science research, using a qualitative approach and a non-experimental emergent design. The participants interviewed for the study consisted of 15 in-service teachers in three schools in Mapuche contexts in the Araucanía region of Chile. The study used a semi-structured interview script to collect data, and in the analysis plan the Grounded Theory (GT) was used, to conceptualize the emerging patterns in categorized concepts and theories. Results: The analysis revealed three distinct categories: culture and diversity; education; and community. Discussion: There is a connection between culture and diversity, education, and community, with learning as the central theme. However, each of these dimensions tends to operate mainly in isolation, lacking effective connections that would facilitate consistent development in the learning and teaching processes. Conclusions: It is essential to incorporate inclusive strategies that recognize and value social and cultural diversity within school classrooms and promoting the dialogue of knowledge and wisdom through intercultural education fosters the active participation of all actors involved in the teaching and learning processes. Full article

The aim of this study was to characterize the vegetative growth development of hop plants grown in the subtropics under a two-crop-a-year system with artificial supplementation lighting. The development of ‘Mapuche’ and ‘Spalter’ hops was compared during the summer 2022–2023, fall 2023, summer 2023–2024 and fall 2024 harvest seasons, considering the effects of the air temperature on the vegetative growth of plants from thermal sums in a subtropical climate region. The experiment was conducted in Palotina, Paraná, Brazil (24°17′40.05″ S, 55°50′23.16″ W, at 332 m elevation). The hops were trained on a 5.5 m high vertical trellis, using a ‘V’-shaped training system. Vegetative growth was evaluated based on the plant height development (m), hop growth rate (HGR), and classification of four growth stages based on the HGR. The phenology of the hop cultivars was determined visually according to the duration in days of the phenological stages. The development of the plant height and HGR was analyzed by nonlinear regressions of the Gompertz model and Gaussian function, respectively. ‘Mapuche’ and ‘Spalter’ hops had complete vegetative growth and phenological phases in the summer and fall seasons, with greater precocity in plant development in the summer season. The growth model based on the air temperature demonstrated that under subtropical conditions, the growth was maximized in seasons with higher temperatures. The duration of the phenological phases and the complete cycle of the plants was influenced by the vegetative growth of each cultivar in each harvest season. Therefore, double annual crop production of the hop cultivars ‘Mapuche’ and ‘Spalter’ is possible in a subtropical climate with artificial light supplementation. Full article

Background/Objectives: Chronic Obstructive Pulmonary Disease (COPD) is one of the most common chronic non-communicable diseases in adults. The most critical risk factors are tobacco and air pollution. The familial aggregation of this disease and the fact that only 15–20% of smokers develop COPD demonstrate the existence of an individual susceptibility that would depend on genetic factors. The already-known susceptibility genomic variants explain only about 38% of the heritability of COPD. The present work analyzes the relationship between the percentage of Amerindian genomic ancestry of Chileans with morbidity and mortality of Chronic Obstructive Pulmonary Disease (COPD), adjusting for socioeconomic and environmental variables. Methods: We rely on the estimates of genomic ancestry percentages obtained in the Chilegenomico project in urban Chileans from 39 communes along eight regions of the country from north to south. From the public databases of the Departamento de Estadísticas e Información en Salud (DEIS) of the Chilean Ministry of Health, we obtained mortality rates and hospital discharge rates. We incorporated adjustment variables (communal data) obtained from other public databases. We performed correlation analyses and fitted negative binomial regression models to examine the association between Amerindian ancestries and COPD statistics. Results: A positive and significant association between Mapuche ancestry and hospital discharge and mortality rates for COPD was found in both simple and multiple analyses. In contrast, we found a negative and significant association between the percentage of Aymara genomic ancestry and COPD mortality rates. Conclusions: The levels of Mapuche and Aymara genomic ancestries have different and contrasting significant associations with COPD susceptibility and mortality in the Chilean mixed population. Full article

Inflammation contributes to the onset and development of many diseases, including neurodegenerative diseases, caused by the activation of microglia, leading to neurological deterioration. Nuclear factor-κB (NF-κB) is one of the most relevant pathways for identifying anti-inflammatory molecules. In this study, polygodial and isotadeonal, two drimane sesquiterpene dialdehydes, were isolated from Drimys winteri, a medicinal tree of the Mapuche people in Chile. Isotadeonal, or epi-polygodial, was obtained from polygodial by epimerization in basic media (60% yield, Na₂CO₃, r/t, 24 h). Both sesquiterpenoids were evaluated on the NF-κB pathway, with the result that isotadeonal inhibited the phosphorylation of IκB-α at 10 μM with higher potency by Western blotting. The final inhibition of the pathway was evaluated using a SEAP reporter (secreted alkaline phosphatase) on THP-1 cells. Isotadeonal inhibited SEAP with higher potency than polygodial, quercetin, and CAPE (phenethyl ester of caffeic acid). In silico analysis suggests that the α-aldehyde of isotadeonal adopts a more stable conformation in the active pocket of IκB-α than polygodial. Full article

Food security remains a critical challenge for older adults in rural Indigenous communities, particularly among the Mapuche people. This study presents an analysis of public policies that address the food security of Mapuche older adults in rural Chile. Using an interpretative qualitative approach, we explore the alignment between government programs and the lived experiences of this population. Findings indicate that existing policies lack cultural pertinence, focusing primarily on market-driven agricultural production rather than self-sufficiency and traditional food systems. Participants highlight the loss of community farming practices, environmental degradation, and the imposition of external production models as key factors exacerbating food insecurity. In addition, health and education policies do not integrate Indigenous knowledge and food habits, which further limits their effectiveness. This study highlights the need for culturally inclusive public policies that support local food sovereignty, sustainable agricultural practices, and the empowerment of Indigenous communities. Full article

Germacranes are a type of sesquiterpene lactones with anti-inflammatory and cytotoxic properties against cancer cell lines. In this in vitro study, erioflorin and erioflorin acetate were isolated and purified from the leaves of Podanthus mitiqui Lindl (Mitique or Mitriu), a shrub endemic to Chile and traditionally used in Mapuche medicine to treat urinary and digestive disorders. Their effects on advanced prostate cancer cell lines (DU-145 and 22Rv1) were evaluated. Cytotoxicity was assessed using real-time cell death and clonogenic assays. Apoptosis was determined by measuring reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm), and apoptotic cell percentage through flow cytometry. Gene expression of BAX and BCL-2 was analyzed via RT-qPCR, while NF-κB activation was studied in DU-145 cells and human monocytic NF-κB reporter assays using LPS stimulation and alkaline phosphatase activity quantification. Erioflorin acetate exhibited the highest cytotoxicity, with IC₅₀ values of 35.1 µM (22Rv1) and 27.3 µM (DU-145), compared to erioflorin, which had IC₅₀ values of 50.3 µM and 56.5 µM, respectively. Both compounds increased ROS levels, reduced ΔΨm, and induced apoptosis. RT-qPCR analysis revealed that erioflorin elevated the BAX/BCL-2 ratio, and both compounds inhibited NF-κB activation by preventing IκBα phosphorylation. In conclusion, the findings demonstrate that erioflorin and erioflorin acetate exert significant in vitro cytotoxic and cytostatic effects on prostate cancer cells, supporting their potential as natural candidates for prostate cancer therapy. Full article

Neurodegenerative diseases are characterized by a progressive process of degeneration and neuronal death in the nervous system, with neuroinflammation being one of the main factors contributing to the progression of these diseases. Aristotelia chilensis (Maqui) is a native tree of Chile used in the Mapuche folk medicine for wounds and digestive treatment. It produces edible black berries with the highest antioxidant capacity among berries, and the Mapuche people used it for producing an alcoholic beverage. The leaves of Maqui contain indole alkaloids with different pharmacological properties that suggest neuroprotective effects. Here, the isolation and chemical characterization of a new alkaloid, named 9-hydroxyaristoquinolone, and the evaluation of its anti-inflammatory activity in the microglial cell line HMC-3, treated with LPS, are reported. 9-Hydroxyaristoquinolone protects microglia from LPS-induced morphological changes at concentrations as low as 1 µM, with a reduction in IKBα-P levels and inhibition of the NF-κB pathway, which was assessed by THP-1 NF-κB dual cell reporter and Western blot in HMC-3 cells. In silico studies suggest that 9-hydroxyaristoquinolone does not induce hepatotoxicity or genotoxicity and exhibits BBB permeability. Full article