Search Results (24)

Geological storage of CO₂ in offshore deep saline aquifers is widely recognized as an effective strategy for large-scale carbon emission reduction. This study aims to assess the mechanical integrity and storage efficiency of reservoirs using a multi-layer CO₂ injection method in the Enping 15-1 Oilfield CO₂ storage project which is the China’s first offshore carbon capture, utilization, and storage (CCUS) demonstration. A coupled Hydro–Mechanical (H–M) model is constructed using the TOUGH-FLAC simulator to simulate a 10-year CO₂ injection scenario, incorporating six vertically distributed reservoir layers. A sensitivity analysis of 14 key geological and geomechanical parameters is performed to identify the dominant factors influencing injection safety and storage capacity. The results show that a total injection rate of 30 kg/s can be sustained over a 10-year period without exceeding mechanical failure thresholds. Reservoirs 3 and 4 exhibit the greatest lateral CO₂ migration distances over the 10-year injection period, indicating that they are the most suitable target layers for CO₂ storage. The sensitivity analysis further reveals that the permeability of the reservoirs and the friction angle of the reservoirs and caprocks are the most critical parameters governing injection performance and mechanical stability. Full article

Hippophae rhamnoides L. is an ecologically and medicinally significant species widely distributed across Eurasia, the suitable habitat of H. rhamnoides subsp. sinensis (is hereinafter referred to as sinensis) is concentrated in Northwest and Southwest China (approximately 34–40° N, 100–115° E). H. rhamnoides subsp. yunnanensis (hereinafter referred to as yunnanensis) is mainly distributed in the Hengduan Mountains and surrounding areas (approximately 25–30° N, 98–103° E). H. rhamnoides subsp. mongolica (hereinafter referred to as mongolica) is native to Central Asia to Siberia and is mainly distributed in Northern Xinjiang and Western Inner Mongolia in China (approximately 40–50° N, 100–120° E). H. rhamnoides subsp. turkestanica (hereinafter referred to as turkestanica) is mainly distributed in Western Xinjiang (approximately 40–45° N, 70–85° E). Climate change poses a considerable challenge, affecting its distribution and leading to shifts in its habitat ranges. This study applies the MaxEnt model to assess climate-driven distribution patterns of Hippophae species in China, and predicts current and future suitable habitats under climate change scenarios. This study employs the MaxEnt model and ArcGIS to simulate the potential distribution of four subspecies of H. rhamnoides during the current period and future projections under scenarios SSP1–2.6 and SSP5–8.5. The analysis reveals that the distributions of sinensis, mongolica, yunnanensis, and turkestanica are influenced primarily by climate variables such as temperature and precipitation, while yunnanensis is predominantly restricted by altitude. Future projections indicate that under the extreme climate of SSP5–8.5, centroid migration will be disrupted; specifically, sinensis is expected to migrate northeast or oscillate, mongolica will expand southwest but be limited by desert steppe zones, and turkestanica may face risks associated with groundwater depletion. This study advocates for integrating climate, ecological, and genetic data into conservation planning, with an emphasis on groundwater restoration and exploring genetic resources for stress resilience. The insights offered here contribute significantly to understanding climate adaptation mechanisms in arid and mountainous ecosystems and guide biodiversity conservation efforts. Full article

In order to conserve valuable soil and water resources and avoid problems related to frozen soil, it is important to study the migration of frozen soil water. A greater understanding of frozen soil–water migration can assist with sustainable development and utilization of soil and water resources in frozen areas. This study used an indoor soil column test device to conduct a one-way indoor freezing test of unsaturated soil and the response of soil sample water migration to different freezing temperatures, initial moisture contents, soil densities, freezing times, solute concentrations, and solute types. The experimental and analytical results showed that the temperature field of the soil sample could be divided into three stages: sharp cooling, slow cooling, and stability. After the soil sample had been frozen for 100 h, the temperature field stabilized. The freezing temperature, initial water content, soil density, and freezing time affected water migration in the soil sample. Lower freezing temperatures and greater initial water content resulted in higher levels of water migration. By contrast, greater soil density led to lower water migration levels. In addition, longer freezing times produced smoother soil–water migration curves. The solute concentration and solute type also affected water migration in frozen soil; the higher the solute concentration, the greater the water migration. Compared with CaCl₂, NaCl had a stronger effect, causing more water migration and leading to a higher water content. The research findings will aid further studies on soil and water utilization, environmental maintenance, and restoration in areas with seasonally frozen soil, as well as promote the sustainable development of agriculture, water conservancy project development, and the social economy. Full article

Digitization has become involved in every aspect of life, including the healthcare sector with its healthcare professionals (HCPs), citizens (patients and their families), and services. This complex process is supported by policies: however, to date, no policy analysis on healthcare digitalization has been conducted in European countries to identify the main goals of digital transformation and its practical implementation. This research aimed to describe and compare the digital health policies across four European countries; namely, their priorities, their implementation in practice, and the digital competencies expected by HCPs. A multiple-case study was performed. Participants were the members of the Digital EducationaL programme invoLVing hEalth profEssionals (DELIVER), a project funded by the European Union under the Erasmus+ programme, involving three countries (Denmark, Italy, and Slovenia) and one autonomous region (Catalonia—Spain). Data were collected using two approaches: (a) a written interview with open-ended questions involving the members of the DELIVER project as key informants; and (b) a policy-document analysis. Interviews were analysed using the textual narrative synthesis and the word cloud policy analysis was conducted according to the Ready, Extract, Analyse and Distil approach. Results showed that all countries had established recent policies at the national level to address the development of digital health and specific governmental bodies were addressing the implementation of the digital transformation with specific ramifications at the regional and local levels. The words “health” and “care” characterized the policy documents of Denmark and Italy (309 and 56 times, 114 and 24 times, respectively), while “development” and “digital” (497 and 478 times, respectively) were common in the Slovenia document. The most used words in the Catalonia policy document were “data” and “system” (570 and 523 times, respectively). The HCP competencies expected are not clearly delineated among countries, and there is no formal plan for their development at the undergraduate, postgraduate, and continuing educational levels. Mutual understanding and exchange of good practices between countries may facilitate the digitalization processes; moreover, concrete actions in the context of HCP migration across Europe for employment purposes, as well as in the context of citizens’ migration for healthcare-seeking purposes are needed to consider the differences emerged across the countries. Full article

(1) Background: The latest research illustrates that microglia phenotype is not the binary ‘resting’ and ‘activated’ profiles. Instead, there is wide diversity in microglia states. Similarly, when testing different stimulation protocols for BV2 microglia, we discovered differences in the response of the cells in terms of the production of intracellular ROS (iROS), nitric oxide (NO), CD40 expression, and migratory capacity. (2) Methods: BV2 microglia were treated with single interferon gamma (IFN-γ) stimulation, LPS/IFN-γ co-stimulation, and priming with IFN-γ followed by stimulation with LPS for 24 h. The responses of BV2 microglia were then assessed using the H₂DCFDA test for iROS, the Griess assay for NO, immunophenotyping for CD40/CD11b/MHC II, and migration using a transwell apparatus. (3) Results: Single stimulation with IFN-γ induced NO but not ROS in BV2 microglia. Co-stimulation with LPS₂₀₀IFN-γ_2.5 induced a higher iROS production (a 9.2-fold increase) and CD40 expression (28031 ± 8810.2 MFI), compared to priming with _primedIFN-γ₅₀LPS₁₀₀ (a 4.0-fold increase in ROS and 16764 ± 1210.8 MFI of CD40). Co-stimulation also induced cell migration. On the other hand, priming BV2 microglia (_primedIFN-γ₅₀LPS₁₀₀) resulted in a higher NO production (64 ± 1.4 µM) compared to LPS₂₀₀IFN-γ_2.5 co-stimulation (44 ± 1.7 µM). Unexpectedly, priming inhibited BV2 migration. (4) Conclusions: Taken together, the findings from this project reveal the ability of co-stimulation and priming in stimulating microglia into an inflammatory phenotype, and the heterogeneity of microglia responses towards different stimulating approaches. Full article

The aim of this study was to evaluate the population characteristics of spirlin, Alburnoides bipunctatus, in Serbia, since this small fish species is facing a severe decline in its abundance and its natural habitats in Europe. We investigated the spirlin population dynamics, including size, age structure, growth pattern, mortality, and exploitation rate. Additionally, we used the Uniform Manifold Approximation and Projection approach with the Decision Tree algorithm to investigate the influence of different environmental parameters on the population parameters to unveil which factors shape the abundance and distribution of spirlin. The results showed that the highest values of production, abundance, and biomass were estimated in sites with low temperature, optimal pH, and well-oxygenated water, even though we found them in heavily polluted waters with extremely high values of conductivity. Moreover, we observed a pattern of migratory behavior, in which spirlin migrate upstream to sites at a higher altitude in early summer and autumn. Despite the putative vulnerability and high sensitivity of spirlin populations, our results showed that the species was abundant, occurring in altered habitats (due to pollution, climate change, anthropogenic pressure, etc.). Full article

Clogging is one of the most important factors that restricts the development and popularization of artificial groundwater recharge technology. Gas clogging is an important but often overlooked form of clogging. In this study, a high-speed image acquisition system was used to obtain high-resolution images of the migration of water and gas in the pore. The bypass flow, trapped bubbles in the H-shaped pore channel, blind end, and corner of the pore were directly observed and their clogging mechanisms were analyzed. The influences of the pore structure and gas content on the degree of gas clogging were quantified. The pore–throat size has a certain controlling effect on the movement of the gas and liquid phases. As the diameter of the pore–throat increases, the clogging effect of the gas decreases, and the relative permeability of the water (k_rw) increases. The pore–throat ratio exhibits a negative correlation with the relative permeability of the liquid phase, and the pore–throat sorting coefficient exhibits a positive correlation with k_rw. As the gas content increases, the degree of gas clogging increases, and the effect is more significant at low gas-to-liquid ratios (<1:2). These results provide theoretical support for the scientific quantitative evaluation and prediction of the occurrence of gas clogging in groundwater recharge projects. Full article

The purpose of this research is to demonstrate the strong potential of Earth-observation (EO) data and techniques in support of migration policies, and to propose actions to fill the existing structural gaps. The work was carried out within the “Smart URBan Solutions for air quality, disasters and city growth” (SMURBS, ERA-PLANET/H2020) project. The novelties introduced by the implemented solutions are based on the exploitation and synergy of data from different EO platforms (satellite, aerial, and in situ). The migration theme is approached from different perspectives. Among these, this study focuses on the design process of an EO-based solution for tailoring and monitoring the SDG 11 indicators in support of those stakeholders involved in migration issues, evaluating the consistency of the obtained results by their compliance with the pursued objective and the current policy framework. Considering the city of Bari (southern Italy) as a case study, significant conclusions were derived with respect to good practices and obstacles during the implementation and application phases. These were considered to deliver an EO-based proposal to address migrants’ inclusion in urban areas, and to unfold the steps needed for replicating the solution in other cities within and outside Europe in a standardized manner. Full article

A ground natural gas leaking event at the Fuling shale gas field is reported in this paper. Thirteen leakage spots were discovered in two places near the SW-1 and SW-2 drilling wells. The biggest leak rate was above 1000 m³ per day, and no H₂S was identified in any of the 13 leaking spots, according to the field study. The chemical components, carbon isotope properties of the leaking gases, and the geological context of the leaking location were researched in order to determine the reason for the leaking incident. From the geological conditions, the Shimen 1# fault belt cuts the whole strata from bottom to top, according to seismic reverse time migration (RTM), and the fault and leaking spots are located in the range of the surface projection of the horizontal section of the adjacent shale gas well. The fracture development evaluation shows that the Wufeng–Longmaxi Formation, which is also the producing layer of the Fuling shale gas field, has a very high possibility of fracture development. From the geochemical view, the carbon isotope of the leaking gases lies in the range of the Wufeng–Longmaxi Formation, showing that the Wufeng–Longmaxi Formation is the gas source of the leaking gas. Full article

Xenoestrogenic effects have been reported in thicklip grey mullet, Chelon labrosus, used as pollution sentinel organisms in estuaries in the Southeast Bay of Biscay with intersex gonads described in populations from some contaminated estuaries. Despite evidence of reproductive stress in this catadromous fish species, knowledge of mullet reproductive movements and connectivity between estuaries is lacking. This study investigates the population structure of C. labrosus using otolith shape and elemental signatures of 60 adult individuals collected from two estuaries found in the Southeast Bay of Biscay (Gernika and Plentzia). All samples were collected in June–July 2020. Otolith shape analysis was determined using elliptical Fourier descriptors, while elemental signatures (Sr:Ca, Li:Ca, Mg:Ca, Mn:Ca, Co:Ca, Ni:Ca, Cu:Ca and Ba:Ca) of whole sagittae were determined by inductively coupled plasma mass spectrophotometry. Both natural tags were analyzed with univariate and multivariate statistics to determine whether these signatures are geographically distinct and can be used to assess the degree of separation between individuals. The data showed significant differences in the otolith shape and elemental analyses, with canonical analysis of principal coordinates plots identifying two different groups, each one belonging to each estuary of origin. Differences in whole otolith elemental signatures between locations were driven by Sr:Ca, Li:Ca, and Ba:Ca. Sr:Ca and Li:Ca ratios were higher in Plentzia than in Gernika, while Ba:Ca was higher in Gernika. The high re-classification success rate using both tools obtained from stepwise linear discriminant function analysis supports these findings and suggests that Gernika and Plentzia individuals passed enough time in separated water compartments and should be regarded as two different population units. This could suggest that the intersex condition in mullets from Gernika is due to life-long exposure to xenoestrogens after homing during early larval development in that estuary, without migrations to other estuaries. Acknowledgements: Project funded by Basque Gov. (IT1302-19), Spanish MCIN and EU-FEDER/ERDF (PGC2018-101442-B-100) and EU H2020 (Assemble+ 730984). Full article

Equid alphaherpesvirus 1 (EHV-1) causes respiratory diseases, abortion, and neurological disorders in horses. Recently, the oncolytic potential of this virus and its possible use in anticancer therapy has been reported, but its influence on cytoskeleton was not evaluated yet. In the following study, we have examined disruptions in actin cytoskeleton of glioblastoma multiforme in vitro model—A172 cell line, caused by EHV-1 infection. We used three EHV-1 strains: two non-neuropathogenic (Jan-E and Rac-H) and one neuropathogenic (EHV-1 26). Immunofluorescent labelling, confocal microscopy, real-time cell growth analysis and Oris^TM cell migration assay revealed disturbed migration of A172 cells infected with the EHV-1, probably due to rearrangement of actin cytoskeleton and the absence of cell projections. All tested strains caused disruption of the actin network and general depolymerization of microfilaments. The qPCR results confirmed the effective replication of EHV-1. Thus, we have demonstrated, for the first time, that EHV-1 infection leads to inhibition of proliferation and migration in A172 cells, which might be promising for new immunotherapy treatment. Full article

The Chagan Lake Catchment is located in the midwest of Songnen Plain, which is a typical high fluoride groundwater area. High fluoride water has an important impact on the economic development and ecosystem stability of Chagan Lake. In this study, the spatial distribution characteristics and influencing factors of fluorine in Chagan Lake Catchment are discussed by using hydrochemistry and mathematical statistical analysis. The groundwater in the study area was characterized as Na⁺-rich and Ca²⁺-poor, with a high pH value and high HCO₃^– content. The average concentration of F^– was 3.02 mg/L, which was the highest in Qian’an County. The dissolution of fluorine-containing minerals and the desorption of F^– in soil provided the source of F^– in groundwater, while calcite and dolomite precipitation, cation exchange, and evaporation concentration provided favorable conditions for F^– dissolving, migration, and enrichment in water. In addition, the concentration of F^– in surface water was 4.56 mg/L, and the highest concentration was found in Hongzi Pool and Hua’ao Pool. The elevated concentrations of F^– in both surface water and groundwater in the study were affected by human factors, such as rice planting and water conservancy project construction. Full article

Cardiotonic steroids (CTS) are specific inhibitors and endogenous ligands of a key enzyme in the CNS—the Na⁺, K⁺-ATPase, which maintains and creates an ion gradient on the plasma membrane of neurons. CTS cause the activation of various signaling cascades and changes in gene expression in neurons and other cell types. It is known that intracerebroventricular injection of cardiotonic steroid ouabain causes mania-like behavior in rodents, in part due to activation of dopamine-related signaling cascades in the dopamine and cAMP-regulated phosphoprotein 32 (DARPP-32) expressing medium spiny neurons in the striatum. Dopaminergic projections in the striatum innervate these GABAergic medium spiny neurons. The objective of this study was to assess changes in the expression of all genes in human iPSC-derived expressing DARPP-32 and GABA receptors neurons under the influence of ouabain. We noted a large number of statistically significant upregulated and downregulated genes after a 16-h incubation with non-toxic concentration (30 nM) of ouabain. These changes in the transcriptional activity were accomplished with activation of MAP-kinase ERK1/2 and transcriptional factor cAMP response element-binding protein (CREB). Thus, it can be concluded that 30 nM ouabain incubated for 16 h with human iPSC-derived expressing DARPP-32 and GABA receptors neurons activates genes associated with neuronal maturation and synapse formation, by increasing the expression of genes associated with translation, vesicular transport, and increased electron transport chain function. At the same time, the expression of genes associated with proliferation, migration, and early development of neurons decreases. These data indicate that non-toxic concentrations of ouabain may induce neuronal maturation, neurite growth, and increased synaptogenesis in dopamine-receptive GABAergic neurons, suggesting formation of plasticity and the establishment of new neuronal junctions. Full article

In the present study, we choose the Weishan Lake, one of important water transfer and storage lakes on the eastern route of the South-to-North Water Diversion Project (SNWD) in China, to clarify how the community structure and assemblage-environment relationships of macroinvertebrates varied across three typical habitats (the River Mouth, Canal and Lake regions) over the four seasons in 2012. A total of 72 taxa belonging to 3 phyla, 9 classes and 24 families were recorded, with tolerant oligochaetes and chironomids as the dominant taxa. The environmental conditions and macroinvertebrate assemblages were clearly separated at spatial and temporal scales. Assemblage structure showed both significant but larger spatial than seasonal variations, with a clear separation of sites from three regions in an ordination plot. Compared to the temporal scale, more indicator species were retained to be responsible for the regional differences according to the two-way cluster analysis. Different environmental variables were significant for distinguishing macroinvertebrate assemblages among four seasons, and among them, pH was the only variable which was retained in all models. Our study provided useful background information of environmental characteristics and macroinvertebrate communities in a typical water transfer and storage lake before the water transfer of the SNWD. After the operation of SNWD, we envisage inter-basin water transfer (IBWT), which is usually accompanied by water level rise, nutrient pattern change and biota succession, will seriously affect recipient basins. Therefore, we propose several management strategies for SNWD: (1) target and detailed data should be collected on a timely basis; (2) government should prevent water pollution and adopt effective measures to protect the water environment; (3) the environmental assessments and other aspects of IBWT planning should be coordinated; (4) an overall consideration of different basins should be given to achieve a greater range of water resources planning, scheduling, and allocation; and (5) the migration and invasion of species should be of concern during the operation of the project. Full article

During the last few years, electric power systems have undergone a widespread shift from conventional fossil-based generation toward renewable energy-based generation. Variable speed wind generators utilizing full-scale power electronics converters are becoming the preferred technology among other types of renewable-based generation, due to the high flexibility to implement different control functions that can support the stabilization of electrical power systems. This paper presents a fundamental study on the enhancement of transient stability in electrical power systems with increasing high share (i.e., above 50%) of power electronic interfaced generation. The wind generator type IV is taken as a representative form of power electronic interfaced generation, and the goal is to investigate how to mitigate the magnitude of the first swing while enhancing the damping of rotor angle oscillations triggered by major electrical disturbances. To perform such mitigation, this paper proposes a power-angle modulation (PAM) controller to adjust the post-fault active power response of the wind generator type IV, after a large disturbance occurs in the system. Based on a small size system, the PAM concept is introduced. The study is performed upon time-domain simulations and analytical formulations of the power transfer equations. Additionally, the IEEE 9 BUS system and the test model of Great Britain’s system are used to further investigate the performance of the PAM controller in a multi-machine context, as well as to perform a comparative assessment of the effect of different fault locations, and the necessary wind generators that should be equipped with PAM controllers. Full article