Search Results (956)

The Western Songnen Plain, a critical yet ecologically fragile grain-producing area, is facing sustainability risks arising from rapid land use changes, which demand scientific assessment and regulation. From an ecological security standpoint, this study synthesizes multiple data sources, including GlobeLand30 data, climate, topography, and soil data. Based on the assessment of water conservation, soil conservation and biodiversity maintenance, combined with minimum cumulative resistance model (MCR) and the CLUMondo model, this study comprehensively reveals the dynamic evolutionary patterns of land use in the Western Songnen Plain over the past two decades, concurrently analyzed the spatial heterogeneity pattern of ecosystem services, and further simulated land use changes under natural growth, farmland protection, and ecological security scenarios. According to the results, the grassland area decreased significantly, while cropland and construction land continued to expand. Water conservation, soil conservation, and habitat quality displayed remarkable regional differences, with high values predominantly situated in wetlands, grasslands, and mountainous regions. In contrast, low values exhibited strong spatial correspondence with regions of heightened anthropogenic disturbance. Although the cropland protection scenario promoted agricultural intensification, it reduced ecological heterogeneity. In contrast, the ecological security scenario achieved a higher patch density (0.408) and landscape diversity (1.142) compared to the natural growth scenario, with moderate increases in aggregation. This study identified 27 ecological pinch points, 24 ecological barrier points, and 97 ecological corridors, which provide direct support for regional water and soil resource protection and further underpin the constructed ecological security pattern of “two belts, three zones, and multiple nodes”. These findings have important reference significance for optimizing regional land use structure and maintaining the stability of terrestrial ecosystems in the Western Songnen Plain. Full article

Salmonella is a major cause of foodborne illness worldwide, posing significant risks to public health and food safety. This study investigated the prevalence, serovar distribution, genotypic characteristics, and antimicrobial resistance (AMR) profiles of Salmonella. A total of 2515 food samples were collected from retail markets, supermarkets, and food processing facilities, and 13,670 stool samples were obtained from sentinel hospitals across 14 cities in Liaoning. The Kruskal–Wallis test was used to compare genetic features among serovars, followed by Dunn’s post hoc test for pairwise comparisons. A total of 314 Salmonella strains were identified, with raw poultry showing the highest detection rate (28.88%) among food sources and children aged 0–6 years (3.47%) the highest among the clinical age groups. Among food samples, S. Enteritidis was the most prevalent serovar (42.6%), and it was also the most common in clinical samples (35.8%); in contrast, S. 4,[5],12:i:- was dominant in pediatric clinical cases. According to AMR analysis, 90.13% of strains were resistant to at least one antibiotic and 67.83% were multidrug-resistant (MDR), with the highest resistance to ampicillin (68.47%). Analysis revealed that S. 4,[5],12:i:- harbored the ASSuT resistance module (blaTEM-1B, aph(3″)-Ib/aph(6)-Id, sul2, tet(B)). Extensive MDR phenotypes were observed in S. Indiana and S. Kentucky, associated with abundant insertion sequences (IS) and resistance genes (ARGs), including clinically critical determinants (blaNDM-9, mcr-1.1, rmtB). The highest mean virulence factor (VF) count (111.17) was observed in S. Enteritidis, contributing to its epidemiological success. Conversely, S. Indiana and S. Kentucky, predominantly food-associated serovars, exhibited reduced virulence but served as critical AMR reservoirs. These findings highlight the epidemiological characteristics and AMR risks of Salmonella in food and clinical settings, providing critical data for food safety and clinical antimicrobial stewardship. Full article

Background: Multidrug-resistant (MDR) Escherichia coli in intensive pig production represents a persistent animal health and One Health concern. Here, we integrated quantitative phenotypic susceptibility data with whole-genome sequencing (WGS) to characterize the resistome and its inferred genomic context (chromosomal vs. plasmid-predicted contigs and mobile genetic element (MGE)-proximal regions) in swine-associated MDR E. coli from Hungary. Methods: A total of 203 E. coli isolates from large-scale pig farms were tested by broth microdilution. Based on resistance-oriented screening from an extended-spectrum β-lactamase (ESBL)-screen-positive pool, 116 isolates were subjected to whole-genome sequencing (WGS) as a resistance-enriched subset. Resistance determinants were annotated using the Comprehensive Antibiotic Resistance Database (CARD). Results: Resistance-oriented screening indicated frequent β-lactamase activity and ESBL screening positivity (110/203 and 127/203 isolates, respectively), consistent with strong antimicrobial selection pressure in the source population. Across the full phenotypic panel, 78/203 isolates (38.4%) met the MDR definition (non-susceptible to ≥3 antimicrobial classes), with marked between-farm variation (p < 0.001) but no age-group effect (p = 0.75). Non-β-lactam minimum inhibitory concentration (MIC) distributions showed pronounced, site-dependent high-MIC “tails”, most notably for tetracyclines, trimethoprim–sulfamethoxazole, fluoroquinolones, and colistin. In the WGS cohort (n = 116), we detected 82 distinct resistance determinants (5433 total occurrences), featuring a conserved chromosomal backbone enriched for intrinsic multidrug resistance components and lipid A modification pathways, alongside common plasmid- and MGE-associated acquired ARG modules involving tetracycline (tetA/tetB), sulfonamide/trimethoprim (sul/dfrA), aminoglycoside-modifying enzymes, and phenicol determinants (floR/cat). High-priority mobile determinants were rare but present, including mcr-1 (3/116; plasmid-associated) and plasmid-mediated quinolone resistance qnrB5 (2/116). Conclusions: Importantly, mobility/context inferences are restricted to this ESBL-screen-enriched WGS subset. Swine-associated E. coli from Hungarian large-scale farms harbors complex resistance architectures shaped by co-selection of mobile ARG modules on top of a pervasive chromosomal resistance backbone. Mobility-aware surveillance and stewardship are warranted to mitigate dissemination risks at the animal–environment–human interface. Full article

This study examines operating-point alignment during full-scale sea trials of a controllable pitch propeller (CPP)-equipped vessel by reconstructing engine load from measured shaft power and relating it to engine performance, fuel-consumption behavior, and combustion indicators. Engine-side performance and fuel-oil consumption records were integrated with shaft measurement data for a MAN 5S35ME-B9.5 low-speed two-stroke marine diesel engine to establish a common propulsion-based operating-point framework. The average shaft power at the 100% speed-trial point was 3471.1 kW, differing from the rated power by only −0.11%, and was adopted as the reference for shaft-load reconstruction. The reconstructed speed-trial operating points were aligned at 24.91%, 49.04%, 80.85%, and 100.00%, while the endurance points corresponded to 76.99% at NCR and 95.29% at MCR. Relative to the corresponding speed-trial references, the endurance points showed about 4.7% lower delivered shaft power, indicating that they should not be interpreted as identical to nominal speed-trial load labels. Fuel flow and combustion-related indicators showed physically consistent variation with increasing reconstructed load. These results demonstrate that measured shaft power provides a practical basis for harmonizing sea-trial datasets and for distinguishing propulsion-side operating conditions more consistently than nominal load labels alone. The proposed framework is particularly applicable to representative operating-point alignment in full-scale sea trials of CPP-equipped low-speed two-stroke marine diesel engines under comparable test conditions. Full article

Antibiotic resistance among bacteria represents a major challenge in modern medicine. The absence of antibiotics effective against multidrug-resistant pathogens triggers interest in reviving older antibiotics. This review aims to provide a focused and updated perspective on the reintroduction of polymyxin antibiotics, with a particular emphasis on colistin, a cyclic oligopeptide initially used in the 1950s and 1960s. We analyze colistin from multiple perspectives, including (i) its historical and contemporary clinical applications, (ii) pharmacokinetic and pharmacodynamic properties, and (iii) use in veterinary medicine and animal husbandry. Key unresolved issues are highlighted, such as colistin toxicity, challenges in susceptibility testing, the emergence of resistance, including the mcr gene variants, and inconsistent clinical evidence supporting combination therapy. By integrating historical background with current data, this review provides a comprehensive overview of the therapeutic relevance, limitations, and ongoing challenges associated with colistin in the era of multidrug-resistant Gram-negative infections. Full article

Background: Uropathogenic Escherichia coli (UPEC) is the primary etiological agent of urinary tract infections (UTIs) worldwide. The emergence of strains combining high virulence with multidrug resistance (MDR) poses a significant challenge to public health. This study aimed to characterize the phylogenetic distribution, virulence profiles, and antimicrobial susceptibility of UPEC isolates recovered from patients in the metropolitan area of Buenos Aires (AMBA), Argentina. Methodology: Phylogenetic groups, the ST131 lineage, and virulence-associated genes were identified using PCR-based assays. Antimicrobial susceptibility testing (AST) was performed using automated methods and extended-spectrum beta-lactamase (ESBL) production was confirmed using the double-disk synergy test. Colistin (COL) resistance was evaluated by Colistin Drop Test and PCR screening for the mcr-1 (mobile colistin resistance gene 1). Biofilm formation was detected by the Tissue Culture Plate (TCP) method, whereas phenotypic virulence factors (VF) were assessed with Congo Red agar, hemagglutination, and hemolysis assays. Results: Phylogenetic groups B2 (43.8%) and D (26.7%), typically associated with extraintestinal infections, were the most frequent. The high-risk clone B2-ST131 was detected in 6.7% of isolates. Biofilm production was observed in 92.4% of the isolates, with curli fimbriae (87.6%) being the most frequently expressed VF. The highest resistance rates were observed for ampicillin (62.1%), ampicillin-sulbactam (39.8%), and trimethoprim-sulfamethoxazole (25.2%). Interestingly, 3.8% of isolates exhibited colistin resistance, despite the absence of the mcr-1 gene. Conclusions: This study highlights the detection of MDR-UPEC isolates that showed strong resistance to fluoroquinolones and were ESBL producers with high virulence in Argentina, justifying future research encompassing genomic and epidemiological monitoring of local UPEC, which is essential for managing infections and developing new therapeutic and preventive measures. Full article

The watershed boundaries in arid and semi-arid regions are critical zones where ecological vulnerability and socio-economic development are in severe conflict. The upper and middle reaches of the Yellow River basin are a typical example of this dilemma. Intensive land use and human developmental interventions in this region have severely disrupted the integrity and balance of the ecosystem. While spatially designated, networked conservation areas can effectively promote the integrity and balance of regional ecosystems, these areas may fail to capture dynamic changes in vulnerability. This study develops a “functional diagnosis-structural diagnosis-integrated optimization” framework. It integrates various scenarios to diagnose vulnerability under uncertainty and identifies bottlenecks in ecological networks. For functional diagnosis, the coupling of the sensitivity–resilience–pressure (SRP) model and the Ordered Weighted Averaging (OWA) algorithm accurately locates vulnerable areas within the regional ecosystem. In terms of structural diagnosis, the Morphological Spatial Pattern Analysis (MSPA), Minimum Cumulative Resistance model (MCR), and Circuit Theory are integrated to identify structural bottlenecks. The main findings of this study are as follows: (1) Functional Diagnosis: The coupling of SRP and OWA reveals the non-linear vulnerability responses to policy preferences and identifies areas that consistently exhibit functional vulnerability across different scenarios. (2) Structural Diagnosis: The circuit theory combined with MSPA and MCR analysis identifies 72 ecological pinch points. These bottlenecks represent the weakest structural nodes crucial for maintaining regional ecological robustness. (3) Coupled Delineation and Differentiated Restoration Strategies: High vulnerability areas identified by SRP and consistently vulnerable areas identified by OWA are combined to delineate four distinct ecological restoration units: Alpine Fragile Matrix Unit, Loess Hilly Soil Conservation Unit, Anthropogenic Pressure Pinch Point Unit, Key Structural Stepping Stone Unit. Differentiated ecological restoration strategies are proposed based on the varying sensitivity, resilience, and pressure characteristics of these units. The “functional-structural” coupled ecological vulnerability evaluation framework can precisely identify vulnerable areas. The delineated restoration units and their corresponding restoration strategies provide reference and supplementation for the protected areas system, offering transferable tools for enhancing regional ecological efficiency. Full article

As a novel approach to address the lack of systematic studies on spatial Gross Ecosystem Product (GEP) accounting and Ecological Security Pattern construction, this study integrates GEP thresholds with Morphological Spatial Pattern Analysis (MSPA) to identify ecological sources. A resistance surface is constructed using five representative influencing factors, and the Minimum Cumulative Resistance (MCR) model is applied to extract ecological corridors, thereby establishing the Ecological Security Pattern for the Yellow River-Fronting Region of Henan in 2020. The results indicate the following: (1) GEP in the study area exhibits a spatial distribution of “high in the northwest, low in the southeast,” with regulating services accounting for more than 90% of the GEP. (2) A total of 11 ecological sources, 13 ecological corridors, and 7 ecological nodes were identified, primarily distributed in mountainous regions. (3) The Ecological Security Pattern exhibits spatial imbalance, with dense corridors in the western mountains and sparse distribution in the eastern plains. These findings provide scientific support for formulating ecological conservation measures and optimizing ecosystem management in the Yellow River Basin. Full article

This study uses Shenyang as a case to integrate multi-source dynamic data with spatial modeling. A comprehensive resistance surface was planned using 12 indicators across the natural, built, and socio-economic dimensions, with objective weighting via the CRITIC method. A hierarchical corridor network was generated based on the MCR model and circuit theory, validated by chi-square goodness-of-fit tests and network structural analysis. The results indicate that socio-economic factors, particularly path activity frequency, dominate the spatial patterns of the corridors, confirming that the network captures connectivity rooted in human activity rather than simply replicating transportation infrastructure. The distribution of national, provincial, and municipal heritage sites across the three higher-importance tiers (L1–L3) shows no significant deviation from the regional baseline, validating the network’s inherent de-hierarchization capacity. Network structure analysis further confirms that this equitable network simultaneously exhibits robust connectivity. The resultant network displays a distinct core–periphery structure with a monocentric-multinuclear radial pattern, forming a four-tier corridor system (core, primary, secondary, and local) that provides an actionable framework for graded protection and targeted interventions. This study advances cultural heritage conservation from passive isolation towards proactive systemic network governance, offering a transferable pathway for the sustainable preservation of heritage in high-density urban environments. Full article

Functionally graded nickel-based coatings represent an advanced surface engineering approach designed to enhance the performance of components operating in high-temperature and harsh environments. Unlike conventional coatings with uniform composition, functionally graded coatings exhibit gradual variations in composition and microstructure across their thickness, enabling improved adhesion, reduced residual stresses, and enhanced multifunctional performance. This review provides a comprehensive overview of recent developments in nickel-based functionally graded coatings, focusing on substrate materials, coating compositions, and manufacturing technologies. Particular attention is given to coatings designed for high-temperature applications and harsh service conditions, including carbide-reinforced composite coatings and MCrAlY-type systems used for oxidation and corrosion protection. Various fabrication methods, including laser cladding, additive manufacturing, electrodeposition, and thermal spraying, are critically discussed in terms of their advantages and limitations. The current state of the art is analyzed with emphasis on coating performance in high-temperature and aggressive environments. Finally, key challenges and future research directions are identified, highlighting the need for improved long-term performance evaluation, advanced manufacturing approaches, and the development of multifunctional gradient coating architectures. Full article

Background/Objectives: Stroke is a leading cause of mortality and disability in Saudi Arabia; however, national estimates of stroke-related rehabilitation needs remain limited. This study quantified temporal trends in stroke incidence, prevalence, premature mortality, and disability from 1990 to 2021. It also examined disparities in stroke-related disability by subtype, sex, and age in 2021 and projected rehabilitation demand to 2030 to inform health system planning under Vision 2030. Methods: We conducted a secondary analysis of Global Burden of Disease (GBD) 2021 estimates for Saudi Arabia. Age-standardized rates for incidence, prevalence, years of life lost (YLLs), and years lived with disability (YLDs) were extracted for overall stroke and three subtypes: ischemic stroke, intracerebral hemorrhage (ICH), and subarachnoid hemorrhage (SAH). Temporal trends were evaluated using log-linear regression to estimate the average annual percentage change (AAPC). YLDs were mapped to severity levels and four rehabilitation modalities, physiotherapy (PT), occupational therapy (OT), speech–language therapy (SLT), and multidisciplinary comprehensive rehabilitation (MCR), using utilization probabilities informed by the literature. Projections to 2030 incorporated national population forecasts and included 95% prediction intervals and sensitivity analyses. Results: From 1990 to 2021, age-standardized stroke incidence declined from 166.3 to 130.7 per 100,000 (−21.4%; AAPC, −0.86%, p = 0.004), prevalence from 982.4 to 965.2 per 100,000 (−1.8%; AAPC, −0.10%, p = 0.056), and YLL rates from 3209.0 to 1893.4 per 100,000 (−41.0%; AAPC, −1.76%, p < 0.001). In contrast, YLD rates declined modestly from 133.5 to 129.9 per 100,000 (−2.7%; AAPC, −0.13%; p = 0.032). Despite these reductions in age-standardized rates, absolute stroke-related YLDs more than tripled, increasing from approximately 10,900 (95% UI: 8100–13,900) in 1990 to 36,245 (95% UI: 26,600–46,100) in 2021, largely driven by population growth and aging. In 2021, ischemic stroke accounted for 71.1% of total YLDs, followed by ICH (20.3%) and SAH (8.5%). Among adults aged 15–49 years, females had higher hemorrhagic YLD rates than males, with particularly pronounced differences for SAH (female-to-male ratio, 1.5–1.7). By 2030, the projected YLD-equivalent workload, a standardized proxy measure of relative service demand rather than a direct headcount of required therapists, is expected to increase to 29,758 for PT, 21,809 for OT, 14,879 for SLT, and 15,083 for MCR. Sensitivity analyses showed that rehabilitation demand estimates were sensitive to assumptions regarding severity distribution, with a hemorrhagic-weighted scenario increasing projected MCR demand by 6.8%. Conclusions: The increasing absolute burden of stroke-related disability in Saudi Arabia, despite declining age-standardized rates and substantial reductions in premature mortality, highlights the necessity to expand rehabilitation capacity. Scaling community-based, outpatient, and telerehabilitation services in alignment with the Health Sector Transformation Program and integrating disability-informed planning into Vision 2030 should be prioritized. Full article

Nanoparticle-catalysed microwave-aided multicomponent reactions (MCRs) have been demonstrated to be competent and environmentally benign tools for the quick synthesis of a wide spectrum of fused heterocyclic systems. The distinctive physicochemical properties of nanoparticles, including a substantial surface area, readily modifiable surface functionality, and heightened catalytic activities, when coupled with microwave irradiation, have enabled a marked improvement in reaction rates, product yields, and selectivity compared to conventional heating methods. This review highlights recent advancements in microwave-assisted MCRs facilitated by diverse nanomaterials, such as magnetic nanocatalysts, metal and metal oxide nanoparticles, mesoporous silica systems, and nanohybrids. It emphasises catalyst design, catalytic efficacy, scope, recyclability, and alignment with green chemistry principles in both solvent-free and aqueous environments, as well as the utilisation of recyclable catalysts. In summary, microwave-assisted multi-component reactions catalysed by nanoparticles are ecofriendly and versatile methods for the sustainable synthesis of such fused heterocycles containing bioactive pyridine, pyrazole, phenazine, pyrimidine, pyran, imidazole, and relevant pyridine derivatives, possessing potential in medicinal and material chemistry. Full article

Brucella anthropi is an emerging opportunistic pathogen characterized by intrinsic resistance to most $\beta$-lactams. However, the acquisition of carbapenem resistance in this species has rarely been documented in environmental, animal, or clinical settings. In this study, a multidrug-resistant strain, SBA01, was isolated from wastewater-irrigated soil. SBA01 exhibited phenotypic resistance to carbapenems and colistin, the latter being independent of mcr genes. Genomic analysis localized bla_IMP-1 on a stable 21 kb plasmid maintained by a Type II toxin–antitoxin system. While non-self-transmissible, this plasmid was mobilized to Escherichia coli and Klebsiella pneumoniae via an unclassified 50 kb helper plasmid. Additionally, a 217 kb prophage-bearing megaplasmid was identified, enhancing genomic plasticity. Genomic screening identified 32 putative virulence determinants, including markers associated with host interaction. Risk profiling indicated an elevated hazard index for SBA01, driven by the convergence of multidrug resistance, cryptic mobilization capacity, and opportunistic survival traits. These findings position B. anthropi as a resilient environmental reservoir for clinically relevant carbapenemases. Expanding surveillance frameworks to include such adaptive hosts is necessary to better evaluate potential occupational exposures at the wastewater–soil interface. Full article

This study employs bibliometric analysis, utilizing the visualization tools CiteSpace 6.3.R1 and VOSviewer 1.6.18, to systematically examine 8727 documents from the Web of Science Core Collection (2000–2024) related to “resilient cities” and “urban resilience.” It explores the evolution of resilient city research, current international trends, practical developments in China, and future directions. The study addresses key questions concerning the theoretical foundations of resilient cities, research advances in the security field, China’s implementation pathways, and emerging trends. Findings indicate that resilient city discourse has evolved from a narrow focus on engineering-based disaster prevention toward a multidimensional, socio-ecological–economic adaptive system. This progression can be divided into three phases: the theoretical foundation period (2000–2008), the technological integration period (2009–2018), and the complex crisis response period (2019–present). Internationally, practices are increasingly centered on climate change adaptation, supported by multi-level governance frameworks such as the MCR2030 initiative. China demonstrates a “dual-track” approach that combines policy-driven initiatives with localized innovations, advancing through international pilot projects, domestic policy experimentation, and grassroots exploration. The study also highlights differences between Chinese and Western research in perspectives, methodologies, and theoretical frameworks. Future resilient city development is expected to emphasize systematization, digitalization, and equity, leveraging technologies such as digital twins and artificial intelligence while fostering community participation and multi-scale collaborative governance. By systematically outlining the theoretical evolution and practical logic of resilient cities, this study offers insights for urban resilience building in developing countries and provides a methodological reference for enhancing resilience capabilities across different administrative levels. Full article

Against the backdrop of a gradual shift in the focus of cultural heritage (CH) conservation and utilization toward the integrated system formed by CH and its surrounding environment as well as regional systems, research on the coordinated protection of nature and culture to promote regional high-quality development has become a new trend. However, systematic summaries of the spatial–temporal distribution of CH in cross-regional typical geomorphic units at the river basin scale and their correlation with the natural environment remain insufficient. This study takes 387 Cultural Relics Protection Units in the Three Gorges of the Yangtze River (the Three Gorges region) as the research objects, utilizing GIS spatial analysis technology to examine the impact of the natural environment on CH across different periods and types. The theory of time-depth is introduced to reveal the layering mechanisms and underlying cultural logics. Coupled with the Minimum Cumulative Resistance (MCR) model, this study constructs a cultural corridor network and proposes spatial planning strategies. The findings are as follows: (1) The absolute core area for the distribution of CH across all periods remains the gentle slope zone near the river, characterized by elevations below 500 m, slopes within 25°, and distances from water systems within 1 km. However, the adaptive scope exhibits a diachronic evolution from core accumulation to peripheral expansion. (2) Different types of CH exhibited distinct natural adaptation strategies and vertical accumulation. Settlement Sites in the Before Qin Dynasty Period formed the foundational layer of survival rationality, while Ordinary Tombs in the Qin–Yuan Dynasty Period reinforced sedentism. Ancient Architecture in the Ming–Qing Dynasty Period underwent a transformation from “adapting to nature” to “reconstructing nature” as a product of environmental construction. Modern and Contemporary Significant Historical Sites and Representative Buildings in the After Qing Dynasty Period are characterized by a ruptured insertion on steep slopes, inscribing revolutionary memory onto space. The main stream of the Yangtze River serves as the core area of continuous deposition, while the extremely steep slopes form a distinctive stratigraphic accumulation of precipitous terrain. (3) Based on these distribution patterns, the study further proposes a spatial framework for CH called “One Corridor, Three Wings.” This framework uses the main stream of the Yangtze River as the spatial–temporal axis, linking the four core overlapping nodes of Fengjie, Wushan, Badong, and Xiling, supplemented by three secondary cultural clusters of the red heritage sites in southern Badong, the ancient town along the Daning River in Wushan, and the fortress sites in the Xiling–Yiling area. This research not only reveals the evolutionary path of CH in the Three Gorges region, but also provides a scientific basis for the systematic conservation and differentiated utilization of regional CH. Furthermore, it serves as a planning foundation and strategic reference for planning the Yangtze River National Cultural Park, as well as for the integrated preservation and utilization of river basin CH and linear CH with the aim of coordinated natural and cultural conservation. Full article