Search Results (11,084)

The transport sector is among the largest final energy consumers and greenhouse gas (GHG) emitters in the European Union. Consequently, reducing energy-related externalities has become a central objective in the EU’s sustainability and decarbonisation policies. This study quantifies the disutility costs associated with energy consumption and emissions across major passenger transport modes—cars, buses, and trains—using a harmonised dataset encompassing 28 EU countries. To do so, a comprehensive disutility cost framework is established, integrating time losses, monetary costs, infrastructure requirements, noise, local air pollutants, and GHG emissions, and combining correlation, regression, and clustering analyses. The results indicate that car transport incurs the highest transport disutility costs, primarily due to congestion-related energy inefficiencies and GHG emissions. In contrast, rail transport demonstrates the lowest cost, energy- and emission-related disutilities across most EU countries. Bus transport represents an intermediate solution, providing lower emission intensity compared to cars but exhibiting higher energy-related disutilities than rail systems. The findings highlight that a modal shift toward rail- and bus-based transport systems can substantially reduce transport-related energy demand, emissions, and income expenses with transport cost at the EU level. While transport innovations and digitalisation may improve system efficiency, their benefits are unevenly distributed, suggesting that energy-focused transport policies should be complemented by measures to ensure inclusive access to low-emission mobility solutions. Full article

The well-being of healthcare professionals (HCPs) is widely recognised as a critical construct related to workforce sustainability, patient safety, and healthcare system performance; however, research in this area remains conceptually fragmented. This systematic review identifies and critically analyses conceptual models, assessment instruments, and factors associated with HCPs’ subjective well-being. A comprehensive literature search was conducted across six databases covering 2014 to 2024, focusing on quantitative empirical studies published in English in peer-reviewed journals. The review adhered to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Study quality was assessed using the Joanna Briggs Institute criteria, and the methodological quality of measurement instruments was evaluated with the Consensus-based Standards for the Selection of Health Measurement Instruments (COSMIN) checklist in validation-focused studies. Of the 7838 records initially identified, 48 studies met the inclusion criteria. Three primary thematic areas emerged: (1) conceptual models framing subjective well-being, (2) measurement instruments assessing subjective well-being, and (3) factors associated with subjective well-being among HCPs. Frequently applied conceptual frameworks included the job demands–resources model, Maslach burnout theory, and WHOQOL-related approaches. Commonly used instruments comprised the WHO-5, Maslach Burnout Inventory, and Mini-Z. In validation-focused studies assessed using COSMIN criteria, internal consistency and aspects of construct validity were generally reported as acceptable; however, reporting across measurement property domains was variable. Factors examined in relation to subjective well-being included workload, emotional exhaustion, social support, autonomy, and work–life balance. Overall, the reviewed literature demonstrates substantial variability in conceptual and methodological approaches and frequently focuses on single dimensions of well-being. These findings highlight the potential value of developing integrated, sector-specific frameworks to inform future measurement development and research in this field. Full article

Most polyphenols (and tannins in their composition), secondary plant metabolites with positive effects on the human body, are soluble in water, which makes them environmentally friendly and the most accessible solvent in everyday life. The aim of this study was to examine the effects of water extraction methods, hot water extraction and maceration, on the amounts of these compounds isolated from plants, compared with ultrasonic extraction, which is not readily available. Seven Ericaceae and four Rosaceae species were selected for study, whose leaves are used in folk and/or official medicine to make herbal teas. Total polyphenolics were determined by the Folin–Ciocalteu method spectrophotometrically and total tannins by calculating the difference between the total and remaining polyphenolic content after tannin precipitation. The results demonstrated that ultrasound was not the most effective method for extracting polyphenols: it yielded the highest polyphenol amounts only from two Rosaceae species, Potentilla anserina and Alchemilla vulgaris. The hot water extraction of polyphenols was more effective than maceration. Hot water was more effective in extracting polyphenols from evergreen plants. Regardless of the extraction method, most of the polyphenols were extracted with water from Arctostaphylos uva-ursi and tannins from Rhododendron tomentosum leaves. The studied Ericaceae species accumulate higher-polarity tannins than the studied Rosaceae representatives. Full article

Background and Objectives: Depression has emerged in recent years as a significant global health issue, drawing considerable research interest and attention. The development of depression could be impacted by a range of environmental factors. Aim: To investigate the relationship between depressive symptoms and various indoor environmental factors, such as microclimate, odors, mold, and room ventilation, in association with some sociodemographic and lifestyle factors. Materials and Methods: This epidemiological health survey of the study “Chronic diseases and their risk factors in the adult population” was performed during 2023–2024 in Kaunas city (Lithuania) following the methodology of the WHO MONICA study. A random sample of Kaunas inhabitants aged 25–69 years, stratified by sex and age, was randomly selected from the Lithuanian population register. The 3426 individuals were screened. The associations of various indoor environmental factors with depressive symptoms were investigated using binary logistic regression analysis. Results: Depressive symptoms were associated with sociodemographic, lifestyle, and indoor environmental factors. Poor microclimate conditions, unpleasant household odors, mold exposure, and insufficient room ventilation were associated with increased odds of depressive symptoms. The significance of these associations varied across sex, age, marital status, socioeconomic status, and physical activity of responders. Additional multivariable logistic regression analyses, including interaction terms between each indoor environmental factor and the stratification variables (sex, age groups, marital status, family economic situation, and physical activity), were performed. Significant interaction was found only between family status and room ventilation (p = 0.007). This indicates that the association between ventilation and depressive symptoms differed by family status. Conclusions: This study contributes to the cross-disciplinary understanding of the role of indoor environmental quality, sociodemographic, and lifestyle factors in the development of depression, adding to the evidence on the role of other factors in depression inequalities. Full article

This study presents the design, implementation, and validation of a thermoelectric energy harvesting system that exploits waste heat from an industrial electric motor to power an autonomous wireless sensor device. The proposed prototype integrates a single thermoelectric generator directly onto the motor housing and leverages the built-in cooling fan to maintain a stable thermal gradient of approximately 4–5 °C. Under real factory conditions, the system harvested 6.17 J of energy over 9612 s, sustaining continuous operation and 41 successful Long Range (LoRa) data transmissions with a positive energy balance. Compared with related works, the prototype achieved competitive or superior performance while operating at a lower motor rating of 0.25 kW, highlighting its efficiency relative to system scale. Key innovations include a hybrid DC/DC conversion chain bridging ultra-low input voltages to modern microcontrollers, and an adaptive transmission strategy that ensures predictable energy management and reliable wireless communication. These results demonstrate the feasibility of battery-free sensing in industrial environments and underline the potential of thermoelectric harvesting as a cost-effective, maintenance-free, and environmentally responsible solution for predictive maintenance and Industry 4.0 applications. Full article

Background/Objectives: Posterior reversible encephalopathy syndrome (PRES) is a neurological condition characterized by acute neurological symptoms and vasogenic edema, usually affecting the posterior circulation. It is described in end-stage renal disease (ESRD), but its presentation in peritoneal dialysis (PD) is not well defined. We aimed to describe the clinical, radiological, and dialysis-related features of PRES in PD patients and highlight factors relevant for diagnosis and management. Materials and Methods: We conducted a retrospective descriptive case series of four ESRD patients on PD or recently transitioned from PD to hemodialysis (HD) who developed PRES at a single center. Clinical data, laboratory results, dialysis characteristics, and neuroimaging findings were obtained from medical records. PRES was diagnosed based on acute neurological symptoms in the setting of severe hypertension and uremia, with CT and/or MRI findings supportive of PRES when present and exclusion of alternative diagnoses. Results: All patients presented with acute neurological manifestations, including headache, visual disturbances, seizures, and/or altered consciousness, in the context of marked hypertension and uremia. Neuroimaging findings ranged from normal CT/MRI to subtle bilateral occipital hypodensities and, in one case, extensive supra- and infratentorial vasogenic edema with internal hydrocephalus and subependymal edema. In three patients, inadequate volume or solute control on PD prompted temporary or permanent transition to HD to improve blood pressure and fluid management. With antihypertensive therapy, seizure control when required, correction of metabolic disturbances, and optimization of dialysis, all patients recovered clinically, with time to PRES resolution ranging from 7 to 43 days. Conclusions: PRES should be considered in PD patients with new-onset seizures, visual symptoms, or unexplained changes in mental status, particularly during hypertensive crises and uremia. Early CT/MRI, prompt blood pressure control, and careful adjustment of dialysis modality appear important for achieving favorable neurological outcomes. Full article

Background/Objectives: Among vat polymerization technologies, liquid-crystal display (LCD) 3D printing has gained popularity in dentistry because of its affordability and acceptable resolution. However, the factors influencing the dimensional accuracy of LCD-printed surgical splints, particularly build platform position, remain insufficiently investigated. This study aimed to evaluate the influence of build platform position on the trueness and precision of orthognathic surgical splints fabricated using LCD 3D printing technology. Methods: Thirty-six surgical splints were printed from a master digital file using an LCD 3D printer. All surgical splints were printed with a 90-degree layer orientation to the building platform. The layer thickness was set at 100 μm. The surgical splints were divided into three groups according to their printing position on the building platform: middle (M), left (Lt), and right (Rt). Each 3D-printed surgical splint was sprayed with an opaque scanning spray and then rescanned to create digital files for testing. A surface-based superimposition and deviation analysis was performed using specialized 3D software to evaluate accuracy of surgical splints. Root mean square error (RMSE) values were statistically analyzed. Results: There were no statistically significant differences in trueness among the middle, left, and right printing positions on the build platform (p > 0.05). In contrast, printing position significantly affected precision, with surgical splints printed at the center of the build platform demonstrating significantly lower RMSE values compared with those printed at the left and right positions (p < 0.001). In addition, no significant difference in precision was detected between the left and right positions. Conclusions: The printing position on the build platform significantly influences the precision of orthognathic surgical splints fabricated using LCD 3D printing technology. Splints printed at the central region of the build platform exhibited the highest precision, whereas trueness was not significantly affected by printing position. These findings suggest that preferential placement of surgical splints at the center of the build platform may improve fabrication consistency and predictability in digital orthognathic surgery workflows. Full article

Proliferating cell nuclear antigen (PCNA) is a critical regulator of DNA replication and repair, and its cancer-associated isoforms represent promising therapeutic targets. The small molecule AOH1996 has been previously reported as a PCNA inhibitor with potent antiproliferative activity. Here, a series of novel AOH1996-based structural analogs were synthesized using structure–activity relationship (SAR) and scaffold-hopping strategies, including 1,2,3-triazole, glycine, and amide derivatives with diverse aromatic and polar substituents. The antiproliferative activity of these compounds was evaluated in MCF-7 (breast cancer) and U87 (glioblastoma) cell lines using the MTT assay. The parent compound AOH1996 exhibited the strongest cytotoxicity, reducing cell viability below 30% at 10 μM. Among the analogs, compounds 1f, 2b, 3b, 3c, and 3d demonstrated significant activity, reducing MCF-7 viability by 60–70% and U87 viability to 30–40% at 10 μM. SAR analysis revealed that electron-withdrawing or moderately lipophilic substituents on the amide side chain and aromatic extensions on the triazole ring enhanced potency, while bulky or strongly electron-donating groups diminished activity. ADMET predictions indicated that most derivatives possessed favorable drug likeness and absorption potential, but high plasma protein binding, short predicted half-lives, and potential cardiotoxicity represent limitations that will require further optimization. Several active compounds were predicted to inhibit P-glycoprotein, suggesting their potential to overcome multidrug resistance. Overall, compounds 2b and 3b showed relatively favorable predicted profiles and can serve as useful lead scaffolds for further optimization and experimental validation. Full article

The bovine uterus, once considered sterile, is now recognized as a dynamic microbial ecosystem that undergoes substantial changes during the postpartum period and plays a critical role in reproductive health. This study investigated the composition and temporal dynamics of the uterine bacterial and fungal microbiota in postpartum Angus cows and showed that dietary grape pomace bolus supplementation was associated with temporal shifts in microbial community composition, including changes in selected bacterial taxa and increased fungal community stability. A total of 19 cows were allocated to control (n = 10) or treatment groups (n = 9), with treated animals receiving slow-release grape pomace boluses administered every three weeks from 21 days prepartum until 60–70 days postpartum. Uterine lavage samples were collected during the first (days 4–12) and ninth (days 63–70) postpartum weeks and analyzed using amplicon-based sequencing targeting the bacterial 16S rRNA (V3–V4) region and the fungal ITS2 region. The uterine microbiota exhibited pronounced temporal changes, with Firmicutes predominating in the early postpartum period and an increased abundance of potentially pathogenic Fusobacteria and Bacteroidetes observed by week nine postpartum, particularly in control cows. Grape pomace supplementation was associated with a more favorable bacterial profile, including a reduced relative abundance of key uterine pathogens such as Fusobacterium necrophorum and Trueperella pyogenes. The uterine mycobiome was dominated by Ascomycota across all groups; however, supplemented cows showed greater fungal community stability and a lower prevalence of opportunistic genera, including Candida spp. In conclusion, dietary grape pomace supplementation may beneficially modulate both bacterial and fungal uterine microbiota during the postpartum period, suggesting its potential as a natural strategy to support uterine microbial homeostasis and reproductive health in beef cows. Full article

Legumes are among the most important plants capable of biological nitrogen fixation. However, there is a significant knowledge gap regarding the specifics of cultivating legumes in hydroponic systems under controlled environment conditions, particularly nitrogen metabolism at different growth stages, which this study addressed. Chickpeas, faba beans, lentils, soybeans, and sugar peas were cultivated in deep-water hydroponics without rhizobia, with a nutrient solution as the nitrogen source. The legumes displayed significant variations in growth patterns and nitrogen dynamics. Among them, soybeans had the longest growth cycle, characterised by extended vegetative and early reproductive phases, while sugar peas developed the fastest. In all species, nitrate was the dominant form of nitrogen found in the roots, stems, and leaves, followed by ammonium (NH₃–N) and nitrite (NO₂⁻). The levels of NH₃–N varied among species, peaking early in faba beans and later in chickpeas. NO₂⁻ concentrations were low and decreased with development. The activities of nitrate reductase and nitrite reductase also varied across species, plant organs, and growth stages. The highest enzyme activity was consistently observed in the leaves. Notably, peas exhibited high enzyme activity across all organs, while the leaves of soybeans showed the highest activity in the studied legumes. Full article

The growing demand for sustainable protein alternatives has increased interest in underutilized plant biomasses with high nutritional potential. This study investigated the conversion efficiency of alfalfa (Medicago sativa L.) and duckweed (Lemna minor L.) proteins through multienzyme hydrolysis, with the aim of evaluating how carbohydrate–protein matrix interactions influence enzymatic accessibility and apparent protein digestibility. Three biotechnological hydrolysis schemes were applied, involving combinations of α-amylase, amyloglucosidase, protease, pepsin, pancreatin, and bile salts, including an in vitro gastrointestinal digestion simulation. The first hydrolysis scheme demonstrated that starch-rich matrices formed a viscous medium that reduced protease mobility and limited protein cleavage. Improved substrate accessibility was achieved when plant material was pre-treated with amylolytic and proteolytic enzymes, which resulted in a noticeably higher release of free amino acids. Amino acid profiling revealed that this enzymatic sequence was the most effective for disrupting carbohydrate-associated protein fractions in both species. In vitro digestion assays indicated higher apparent protein conversion for duckweed compared to alfalfa under standardized laboratory conditions. Overall, the results confirm that appropriate multienzyme strategies can enhance amino acid liberation from complex plant matrices and highlight duckweed biomass as a promising candidate for sustainable protein valorization. Full article

Pro-environmental behaviour (PEB) and its factors are widely analysed in the scientific literature to better understand individuals’ decisions and behavioural patterns in the environmental context. PEB is essential for achieving the European Union’s (EU) climate and sustainability objectives; however, significant differences persist between Western and Eastern member states. The article aims to assess factors and identify differences that determine pro-environmental behaviour in the Western and Eastern European Union countries. Particular attention is paid to the role of subjective well-being (SWB) and demographic and structural factors in shaping different dimensions of PEB at the regional level. This study examines the factors of PEB across these two regions using data from the European Social Survey (ESS). The empirical analysis is based on the application of mixed methods, including regression analysis and cluster analysis, which allows for the identification of regional patterns of behaviour and country profiles. Grounded on mixed-method empirical analyses, the findings demonstrate that pro-environmental behaviour and climate responsibility are shaped by distinct motivational mechanisms that vary systematically across European regions. SWB plays a stronger internal motivational role in Eastern Europe, while education and gender dominate in Western Europe. This study contributes to sustainability research by providing a comparative, interregional perspective and emphasising the need for differentiated policy strategies that take into account regional differences in behavioural motivations and structural conditions. The results of this study can be applied in developing regionally adaptable environmental and climate policy measures focused on different behavioural motivation, which will increase policy effectiveness and public engagement in both Eastern and Western EU countries. Full article

The rapid growth of renewable electricity generation introduces technical challenges that were previously uncommon. These include, for example, problems with exceeding the permissible voltage values in network nodes, overloading of transformers and line sections located behind the transformer, as well as balance problems. This article proposes an original methodology for eliminating these problems. Four objective functions reflecting different operator priorities were used. Attention is drawn to the increasing importance of the development of electricity storage. The results confirm that coordinated optimisation of voltage regulation, energy storage, and flexible load management enables increased renewable energy connection capacity while reducing power losses and improving the grid voltage profile. The case study results demonstrate the effectiveness of the proposed approach under the considered operating scenarios. The proposed tool can support network operators in managing MV grid operation under the considered scenarios. The ongoing energy transition requires network operators to react quickly to emerging problems. Therefore, advanced computational methods are needed to mitigate operational risks and respond to emerging constraints. Full article

Climate change is reshaping Antarctic ecosystems, where the resilience of Deschampsia antarctica and Colobanthus quitensis is mediated by endophytic microbial communities assembled under strong abiotic drivers. This study explores the temperature-dependent biofilm development in two Antarctic endophytic microbial communities (ALS and LS). Multivariate analysis revealed a fundamental trade-off between planktonic expansion and biofilm matrix investment as a function of thermal cues. While moderate warming (15–25 °C) optimized cell viability and turbidity, extreme thermal stress at 37–42 °C in nutrient-rich conditions triggered a significant shift toward a matrix-rich signature, characterized by a synergistic increase in total DNA and cellulose. Crucially, at the thermal extreme of 42 °C, we observed a decoupling of optical density from culturable biomass, where high turbidity did not translate into viable cells, signaling a state of severe environmental stress. These results identify 25 °C as the quantitative threshold for optimal growth, while temperatures of 37–42 °C act as a specific trigger for protective matrix production. Such thermal plasticity suggests that Antarctic endophytes are evolutionarily primed for persistence not only in cold native niches but also during bird-mediated dispersal at endothermic host temperatures. Full article